Rorug04G0357900

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001217
Physical Location & Seq
Forward (+)
53102593 .. 53105518
2926 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0357900.1

Sequence Viewer

Length: 1965 bp
ATGAAACATCAAAACATGTTCTTCTTCCCCCCATTCATAAACCTCTATGTTCTCATTGTCTTTCTCTTATCAGAAACCTCATTTGCTGTGGACTCTCACTACCAAAACTGCAGTGTGCCCAAGTCTTGTGGCGGCCAAAACATAAGCTTTCCGTTCTTCATCCCAGGCCTGCAAGAAGACTACTGCGGCTTTCCGGGGTTTCAGCTCTCCTGCAATGATAATGAAGAAGGTGGATACCCTACTCTCCAATTGGCTGGAAATGAGTACTTAGTCCACAATATCAATTACCAAAACCAAACTCTTGTGGTTTCAAACGCTGCTCTTTCAAACTCAAACACAGATGTTTGTATTCCATTAGTCCATAACTCAACCTTTCCTACTATTCAGTATGCCCTTGTTCCGAATCAAAAAGAGATCGTTTTGCTCTACTGCAACTCTTCTCTGGTTGATGAGTCGTTTTTAGAGTACAAGATTGGCTGCTTTGAGCCAAGCAGTAGGACTAATACTTCAATTTTGGCGCTGCCTCGAGATGATGACCAACTATTTGATGATGTGTCTGACAAGTGCGGACGCAAAGTGGTGGTGGTGGCTCCGGTTGAAGAATCAGTAAGTAATGTTAGGAATGAGTTGGGGGTAGCAGACGCGTTGAGAAGAGGGTTTATGCTGAAATGGCTTGCAAGCGACTGCAGTCGCTGCAGAGACAGTGGAGGGAAGTGTGGTTTCGATTTCAAGACGTATCTTTTCAGCTGTTTCTGCCCAGATAGGCCTCATGCTGTCAGCTGTAAAGGTAAAGGAGTTGCATTGGGATTGAAGCTAGGACTAGGCCTAGGACTAGCAGGTGCAGGTGGTCTTATGATTCTAGTACTAGTTGTTGTTTGCTGCTTCAGGAGAAAGTTGTCTGATAAATCTACTTTCTTTTGGATGAACAAAAATCAAAGTCATCAAATTGTTCAGGAATTTCTAATGGACTATGGACCACTTCAAGTTCAGAGATACAGCTACTTGGATGTGAAGAAAATGACAAACTCCTTCGAGGAAAAATTAGGAGAAGGGGGCTACGGTGGTGTATACAAAGGAAAACTAAACGACGGCTTTCTTGTAGCAGTGAAGATCTTGAACAGATCAAAAGGTAATGGAGAAGAGTTTATGAATGAGGTTGCAGCCATTAGTAGAACTTCTCATGTCAACATTGTCAACTTGCTGGGATTTTGTTTTGAGGGTTCAGAAAGAGCTCTCATCTATGAATTCATGCCTAATGGATCTTTGGAGAAGTTCATATTTGATGCAAATAACCCACGAAAAGATCATCACTTGGGGTGGGAAGCATTGGATCGAATTGCACTTGGCATTGCTCGAGGACTAGAGTATTTACATCGCGGTTGTAACACAAGAATTTTGCATTTTGATATCAAGCCTCATAACATTCTTCTTAATGAGGACTTCACACCGAAAATCTCAGATTTCGGCCTGGCTAAAATATGCAACGGAAAAGAGAGTATTGTGTCAATGATGGGTGCAAGAGGAACTGCTGGGTACATTGCTCCTGAAGTGTTTTGTAGAAATTTTGGTGGTATCTCGCACAAATCTGATGTGTATAGCTATGGAATGATGCTTTCGGAGATGGTTGGGGGAAGAAGGAACATCGGTGTTGAAGTTGAAGTTGAAGATACAAGTGAGATATACTTTCCACAATGGATTTACAAGCGTCTTGAATTGGATCAAGAACTTGGCCTACAGAGGATCATGAATGAGGAAGACAAAGGCAGAGCAAGGAAGATGATAATCGTGAGCTTATGGTGCATACAAACTGATCCCTCAAACCGGCCAGCGATGAAACAAGTCATAGAAATGTTGGAAGGAAGTATTGGATCTTTGCAGATACCACCAAAGCCTTACTTGTATTCTCCTCCAAAATCCCTAGCAGAACCAGCACATCCTTCTTCTACTTTGGTATCACTACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

654

Amino Acids

73.06

Weight (kDa)

6.1

Isoelectric Point (pI)

40.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 37 - 104 2.1e-17 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 180 - 255 4.8e-16 Wall-associated receptor kinase C-terminal
PK_Tyr_Ser-Thr PF07714 344 - 618 8.3e-43 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 345 - 616 2.3e-45 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 827, 833
Acc36I ACCTGC 2 cut(s) 827, 833
AccI GTMKAC 1 cut(s) 1068
AccII CGCG 2 cut(s) 644, 1377
AciI CCGC 4 cut(s) 132, 186, 567, 1377
AclWI GGATC 6 cut(s) 1267, 1338, 1725, 1748, 1805, 1876
AcoI YGGCCR 2 cut(s) 133, 1823
AcsI RAATTY 4 cut(s) 956, 1244, 1392, 1561
AcuI CTGAAG 2 cut(s) 868, 1566
AfaI GTAC 4 cut(s) 266, 467, 864, 1535
AfiI CCNNNNNNNGG 1 cut(s) 1821
AflIII ACRYGT 2 cut(s) 15, 642
AhlI ACTAGT 1 cut(s) 865
AjnI CCWGG 2 cut(s) 163, 1467
AjuI GAANNNNNNNTTGG 2 cut(s) 1848, 1880
AluBI AGCT 9 cut(s) 147, 205, 747, 780, 814, 999, 1232, 1599, 1791
AluI AGCT 9 cut(s) 147, 205, 747, 780, 814, 999, 1232, 1599, 1791
Alw21I GWGCWC 1 cut(s) 1234
Alw26I GTCTC 1 cut(s) 693
AlwI GGATC 6 cut(s) 1267, 1338, 1725, 1748, 1805, 1876
AlwNI CAGNNNCTG 1 cut(s) 693
Ama87I CYCGRG 2 cut(s) 525, 1353
AoxI GGCC 7 cut(s) 133, 166, 764, 823, 1465, 1729, 1823
ApeKI GCWGC 6 cut(s) 317, 477, 520, 693, 879, 1160
ApoI RAATTY 4 cut(s) 956, 1244, 1392, 1561
ArsI GACNNNNNNTTYG 2 cut(s) 527, 559
AspA2I CCTAGG 1 cut(s) 826
AspLEI GCGC 1 cut(s) 520
AspS9I GGNCC 1 cut(s) 974
AsuC2I CCSGG 1 cut(s) 195
AvaI CYCGRG 2 cut(s) 525, 1353
AvaII GGWCC 1 cut(s) 974
AvrII CCTAGG 1 cut(s) 826
BaeGI GKGCMC 1 cut(s) 120
BaeI ACNNNNGTAYC 1 cut(s) 1935
BanII GRGCYC 1 cut(s) 1234
BbsI GAAGAC 2 cut(s) 183, 1761
Bbv12I GWGCWC 1 cut(s) 1234
BbvI GCAGC 6 cut(s) 304, 464, 507, 680, 866, 1172
BccI CCATC 2 cut(s) 1504, 1615
BceAI ACGGC 1 cut(s) 1105
BciT130I CCWGG 2 cut(s) 165, 1469
BciVI GTATCC 1 cut(s) 227
BcnI CCSGG 1 cut(s) 195
BcoDI GTCTC 1 cut(s) 693
BcuI ACTAGT 1 cut(s) 865
BfaI CTAG 8 cut(s) 815, 821, 827, 833, 860, 866, 1361, 1919
BfmI CTRYAG 4 cut(s) 109, 685, 694, 1733
BfoI RGCGCY 1 cut(s) 521
BfuAI ACCTGC 2 cut(s) 827, 833
BfuI GTATCC 1 cut(s) 227
BglII AGATCT 1 cut(s) 1110
BisI GCNGC 8 cut(s) 133, 187, 318, 478, 521, 694, 880, 1161
BlnI CCTAGG 1 cut(s) 826
BlsI GCNGC 8 cut(s) 134, 188, 319, 479, 522, 695, 881, 1162
BmcAI AGTACT 2 cut(s) 266, 864
Bme1390I CCNGG 3 cut(s) 165, 195, 1469
Bme18I GGWCC 1 cut(s) 974
BmeT110I CYCGRG 2 cut(s) 525, 1353
BmgT120I GGNCC 1 cut(s) 974
BmiI GGNNCC 1 cut(s) 591
BmrFI CCNGG 3 cut(s) 165, 195, 1469
BmsI GCATC 2 cut(s) 1273, 1599
BoxI GACNNNNGTC 1 cut(s) 687
BpiI GAAGAC 2 cut(s) 183, 1761
BpuMI CCSGG 1 cut(s) 195
BsaBI GATNNNNATC 1 cut(s) 1781
BsaJI CCNNGG 3 cut(s) 163, 194, 826
BsaWI WCCGGW 1 cut(s) 592
Bsc4I CCNNNNNNNGG 1 cut(s) 1821
Bse118I RCCGGY 1 cut(s) 1821
Bse3DI GCAATG 3 cut(s) 220, 1347, 1536
Bse8I GATNNNNATC 1 cut(s) 1781
BseBI CCWGG 2 cut(s) 165, 1469
BseDI CCNNGG 3 cut(s) 163, 194, 826
BseGI GGATG 4 cut(s) 159, 927, 1012, 1933
BseJI GATNNNNATC 1 cut(s) 1781
BseLI CCNNNNNNNGG 1 cut(s) 1821
BseMI GCAATG 3 cut(s) 220, 1347, 1536
BseMII CTCAG 1 cut(s) 1470
BseRI GAGGAG 1 cut(s) 1896
BseSI GKGCMC 1 cut(s) 120
BseXI GCAGC 6 cut(s) 304, 464, 507, 680, 866, 1172
BseYI CCCAGC 2 cut(s) 1201, 1529
BsgI GTGCAG 1 cut(s) 861
Bsh1236I CGCG 2 cut(s) 644, 1377
BshFI GGCC 7 cut(s) 135, 168, 766, 825, 1467, 1731, 1825
BsiHKAI GWGCWC 1 cut(s) 1234
BsiHKCI CYCGRG 2 cut(s) 525, 1353
BsiSI CCGG 3 cut(s) 194, 593, 1822
BslI CCNNNNNNNGG 1 cut(s) 1821
BsmAI GTCTC 1 cut(s) 693
BsnI GGCC 7 cut(s) 135, 168, 766, 825, 1467, 1731, 1825
BsoBI CYCGRG 2 cut(s) 525, 1353
Bsp1286I GDGCHC 2 cut(s) 120, 1234
BspACI CCGC 4 cut(s) 132, 186, 567, 1377
BspANI GGCC 7 cut(s) 135, 168, 766, 825, 1467, 1731, 1825
BspCNI CTCAG 1 cut(s) 1469
BspFNI CGCG 2 cut(s) 644, 1377
BspHI TCATGA 1 cut(s) 1743
BspLI GGNNCC 1 cut(s) 591
BspMAI CTGCAG 3 cut(s) 113, 689, 698
BspMI ACCTGC 2 cut(s) 827, 833
BspPI GGATC 6 cut(s) 1267, 1338, 1725, 1748, 1805, 1876
BsrDI GCAATG 3 cut(s) 220, 1347, 1536
BsrFI RCCGGY 1 cut(s) 1821
BssAI RCCGGY 1 cut(s) 1821
BssECI CCNNGG 3 cut(s) 163, 194, 826
BssNAI GTATAC 1 cut(s) 1069
BssT1I CCWWGG 1 cut(s) 826
Bst1107I GTATAC 1 cut(s) 1069
Bst2UI CCWGG 2 cut(s) 165, 1469
Bst4CI ACNGT 2 cut(s) 704, 1061
Bst6I CTCTTC 3 cut(s) 442, 646, 1134
BstAPI GCANNNNNTGC 1 cut(s) 693
BstC8I GCNNGC 4 cut(s) 170, 675, 679, 1827
BstDEI CTNAG 2 cut(s) 268, 1456
BstF5I GGATG 4 cut(s) 159, 927, 1012, 1933
BstFNI CGCG 2 cut(s) 644, 1377
BstH2I RGCGCY 1 cut(s) 521
BstHHI GCGC 1 cut(s) 520
BstMAI GTCTC 1 cut(s) 693
BstMWI GCNNNNNNNGC 5 cut(s) 670, 693, 753, 1797, 1928
BstNI CCWGG 2 cut(s) 165, 1469
BstNSI RCATGY 1 cut(s) 19
BstPAI GACNNNNGTC 1 cut(s) 687
BstSCI CCNGG 3 cut(s) 163, 193, 1467
BstSFI CTRYAG 4 cut(s) 109, 685, 694, 1733
BstSLI GKGCMC 1 cut(s) 120
BstUI CGCG 2 cut(s) 644, 1377
BstV1I GCAGC 6 cut(s) 304, 464, 507, 680, 866, 1172
BstV2I GAAGAC 2 cut(s) 183, 1761
BstX2I RGATCY 3 cut(s) 1110, 1259, 1868
BstXI CCANNNNNNTGG 1 cut(s) 254
BstYI RGATCY 3 cut(s) 1110, 1259, 1868
BstZ17I GTATAC 1 cut(s) 1069
BsuI GTATCC 1 cut(s) 227
BsuRI GGCC 7 cut(s) 135, 168, 766, 825, 1467, 1731, 1825
BtgZI GCGATG 2 cut(s) 1358, 1844
BtsCI GGATG 4 cut(s) 159, 927, 1012, 1933
BtsI GCAGTG 2 cut(s) 118, 1110
BtsIMutI CAGTG 3 cut(s) 118, 709, 1110
BveI ACCTGC 2 cut(s) 827, 833
Cac8I GCNNGC 4 cut(s) 170, 675, 679, 1827
CaiI CAGNNNCTG 1 cut(s) 693
CciI TCATGA 1 cut(s) 1743
CfoI GCGC 1 cut(s) 520
Cfr10I RCCGGY 1 cut(s) 1821
Cfr13I GGNCC 1 cut(s) 974
CseI GACGC 3 cut(s) 579, 650, 1694
Csp6I GTAC 4 cut(s) 265, 466, 863, 1534
CspCI CAANNNNNGTGG 2 cut(s) 109, 144
CviAII CATG 5 cut(s) 16, 770, 1181, 1249, 1744
CviQI GTAC 4 cut(s) 265, 466, 863, 1534
DdeI CTNAG 2 cut(s) 268, 1456
EaeI YGGCCR 2 cut(s) 133, 1823
Eam1104I CTCTTC 3 cut(s) 442, 646, 1134
EarI CTCTTC 3 cut(s) 442, 646, 1134
Ecl136II GAGCTC 1 cut(s) 1232
Eco130I CCWWGG 1 cut(s) 826
Eco147I AGGCCT 3 cut(s) 168, 766, 825
Eco24I GRGCYC 1 cut(s) 1234
Eco32I GATATC 1 cut(s) 1408
Eco47I GGWCC 1 cut(s) 974
Eco53kI GAGCTC 1 cut(s) 1232
Eco57I CTGAAG 2 cut(s) 868, 1566
Eco88I CYCGRG 2 cut(s) 525, 1353
EcoICRI GAGCTC 1 cut(s) 1232
EcoRI GAATTC 1 cut(s) 1244
EcoRII CCWGG 2 cut(s) 163, 1467
EcoRV GATATC 1 cut(s) 1408
EcoT14I CCWWGG 1 cut(s) 826
EcoT38I GRGCYC 1 cut(s) 1234
ErhI CCWWGG 1 cut(s) 826
FaeI CATG 5 cut(s) 19, 773, 1184, 1252, 1747
FalI AAGNNNNNCTT 2 cut(s) 1880, 1912
FatI CATG 5 cut(s) 15, 769, 1180, 1248, 1743
FblI GTMKAC 1 cut(s) 1068
Fnu4HI GCNGC 8 cut(s) 133, 187, 318, 478, 521, 694, 880, 1161
FokI GGATG 4 cut(s) 146, 934, 1019, 1920
FriOI GRGCYC 1 cut(s) 1234
Fsp4HI GCNGC 8 cut(s) 133, 187, 318, 478, 521, 694, 880, 1161
FspBI CTAG 8 cut(s) 815, 821, 827, 833, 860, 866, 1361, 1919
GlaI GCGC 1 cut(s) 519
GluI GCNGC 8 cut(s) 133, 187, 318, 478, 521, 694, 880, 1161
GsaI CCCAGC 2 cut(s) 1205, 1533
HaeII RGCGCY 1 cut(s) 521
HaeIII GGCC 7 cut(s) 135, 168, 766, 825, 1467, 1731, 1825
HapII CCGG 3 cut(s) 194, 593, 1822
HgaI GACGC 3 cut(s) 579, 650, 1694
HhaI GCGC 1 cut(s) 520
Hin1II CATG 5 cut(s) 19, 773, 1184, 1252, 1747
Hin6I GCGC 1 cut(s) 518
HinP1I GCGC 1 cut(s) 518
HincII GTYRAC 2 cut(s) 1186, 1195
HindII GTYRAC 2 cut(s) 1186, 1195
HindIII AAGCTT 1 cut(s) 145
HinfI GANTC 5 cut(s) 92, 403, 452, 602, 856
HpaII CCGG 3 cut(s) 194, 593, 1822
Hpy166II GTNNAC 5 cut(s) 91, 274, 1069, 1186, 1195
Hpy188I TCNGA 9 cut(s) 73, 402, 559, 901, 990, 1225, 1459, 1588, 1618
Hpy8I GTNNAC 5 cut(s) 91, 274, 1069, 1186, 1195
Hpy99I CGWCG 1 cut(s) 1091
HpyAV CCTTC 6 cut(s) 221, 1039, 1043, 1629, 1850, 1947
HpyCH4III ACNGT 2 cut(s) 704, 1061
HpyCH4IV ACGT 1 cut(s) 734
HpyF10VI GCNNNNNNNGC 5 cut(s) 670, 693, 753, 1797, 1928
HpyF3I CTNAG 2 cut(s) 268, 1456
HpySE526I ACGT 1 cut(s) 734
Hsp92II CATG 5 cut(s) 19, 773, 1184, 1252, 1747
HspAI GCGC 1 cut(s) 518
LmnI GCTCC 2 cut(s) 595, 1546
Lsp1109I GCAGC 6 cut(s) 304, 464, 507, 680, 866, 1172
LweI GCATC 2 cut(s) 1273, 1599
MaeI CTAG 8 cut(s) 815, 821, 827, 833, 860, 866, 1361, 1919
MaeII ACGT 1 cut(s) 734
MaeIII GTNAC 1 cut(s) 1382
MfeI CAATTG 1 cut(s) 248
MflI RGATCY 3 cut(s) 1110, 1259, 1868
MhlI GDGCHC 2 cut(s) 120, 1234
MluI ACGCGT 1 cut(s) 642
MlyI GAGTC 2 cut(s) 86, 461
MmeI TCCRAC 1 cut(s) 1833
MseI TTAA 1 cut(s) 1431
MslI CAYNNNNRTG 1 cut(s) 1847
MspA1I CMGCKG 2 cut(s) 747, 780
MspI CCGG 3 cut(s) 194, 593, 1822
MspR9I CCNGG 3 cut(s) 165, 195, 1469
MunI CAATTG 1 cut(s) 248
MvaI CCWGG 2 cut(s) 165, 1469
MvnI CGCG 2 cut(s) 644, 1377
MwoI GCNNNNNNNGC 5 cut(s) 670, 693, 753, 1797, 1928
NciI CCSGG 1 cut(s) 195
NlaIII CATG 5 cut(s) 19, 773, 1184, 1252, 1747
NlaIV GGNNCC 1 cut(s) 591
NspI RCATGY 1 cut(s) 19
PaeR7I CTCGAG 2 cut(s) 525, 1353
PagI TCATGA 1 cut(s) 1743
PaqCI CACCTGC 2 cut(s) 827, 833
PceI AGGCCT 3 cut(s) 168, 766, 825
PciI ACATGT 1 cut(s) 15
PfeI GAWTC 3 cut(s) 403, 602, 856
PkrI GCNGC 8 cut(s) 134, 188, 319, 479, 522, 695, 881, 1162
PleI GAGTC 2 cut(s) 86, 460
PpsI GAGTC 2 cut(s) 86, 460
PscI ACATGT 1 cut(s) 15
PshAI GACNNNNGTC 1 cut(s) 687
Psp124BI GAGCTC 1 cut(s) 1234
Psp6I CCWGG 2 cut(s) 163, 1467
PspFI CCCAGC 2 cut(s) 1201, 1529
PspGI CCWGG 2 cut(s) 163, 1467
PspN4I GGNNCC 1 cut(s) 591
PspPI GGNCC 1 cut(s) 974
PspXI VCTCGAGB 1 cut(s) 1353
PsrI GAACNNNNNNTAC 2 cut(s) 1716, 1748
PstI CTGCAG 3 cut(s) 113, 689, 698
PstNI CAGNNNCTG 1 cut(s) 693
PsuI RGATCY 3 cut(s) 1110, 1259, 1868
PvuII CAGCTG 2 cut(s) 747, 780
RsaI GTAC 4 cut(s) 266, 467, 864, 1535
RsaNI GTAC 4 cut(s) 265, 466, 863, 1534
RseI CAYNNNNRTG 1 cut(s) 1847
SacI GAGCTC 1 cut(s) 1234
SaqAI TTAA 1 cut(s) 1431
SatI GCNGC 8 cut(s) 133, 187, 318, 478, 521, 694, 880, 1161
Sau96I GGNCC 1 cut(s) 974
ScaI AGTACT 2 cut(s) 266, 864
SchI GAGTC 2 cut(s) 86, 461
ScrFI CCNGG 3 cut(s) 165, 195, 1469
SduI GDGCHC 2 cut(s) 120, 1234
SfaNI GCATC 2 cut(s) 1273, 1599
SfcI CTRYAG 4 cut(s) 109, 685, 694, 1733
Sfr274I CTCGAG 2 cut(s) 525, 1353
SinI GGWCC 1 cut(s) 974
SlaI CTCGAG 2 cut(s) 525, 1353
SmiMI CAYNNNNRTG 1 cut(s) 1847
SmlI CTYRAG 2 cut(s) 525, 1353
SmoI CTYRAG 2 cut(s) 525, 1353
SpeI ACTAGT 1 cut(s) 865
SseBI AGGCCT 3 cut(s) 168, 766, 825
SsiI CCGC 4 cut(s) 132, 186, 567, 1377
SspMI CTAG 8 cut(s) 815, 821, 827, 833, 860, 866, 1361, 1919
SstI GAGCTC 1 cut(s) 1234
StuI AGGCCT 3 cut(s) 168, 766, 825
StyD4I CCNGG 3 cut(s) 163, 193, 1467
StyI CCWWGG 1 cut(s) 826
TaaI ACNGT 2 cut(s) 704, 1061
TaiI ACGT 1 cut(s) 737
TaqI TCGA 5 cut(s) 526, 723, 1032, 1333, 1354
TatI WGTACW 3 cut(s) 264, 465, 862
TauI GCSGC 2 cut(s) 135, 189
TfiI GAWTC 3 cut(s) 403, 602, 856
Tru1I TTAA 1 cut(s) 1431
Tru9I TTAA 1 cut(s) 1431
TscAI CASTG 3 cut(s) 118, 709, 1110
TseI GCWGC 6 cut(s) 317, 477, 520, 693, 879, 1160
TspGWI ACGGA 2 cut(s) 141, 1500
TspRI CASTG 3 cut(s) 118, 709, 1110
VpaK11BI GGWCC 1 cut(s) 974
XapI RAATTY 4 cut(s) 956, 1244, 1392, 1561
XceI RCATGY 1 cut(s) 19
XhoI CTCGAG 2 cut(s) 525, 1353
XmaJI CCTAGG 1 cut(s) 826
XmiI GTMKAC 1 cut(s) 1068
XspI CTAG 8 cut(s) 815, 821, 827, 833, 860, 866, 1361, 1919
ZrmI AGTACT 2 cut(s) 266, 864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.