Belongs to the disease resistance NB-LRR family
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| TIR | PF01582 | 23 - 188 | 2.3e-40 | TIR domain |
| TIR_2 | PF13676 | 25 - 118 | 9.8e-17 | TIR domain |
| NB-ARC | PF00931 | 201 - 370 | 4.9e-20 | NB-ARC domain |
| ATPase_2 | PF01637 | 201 - 400 | 1.9e-07 | ATPase domain predominantly from Archaea |
| NACHT | PF05729 | 218 - 369 | 1.5e-06 | NACHT domain |
| WHD_ROQ1 | PF23282 | 443 - 513 | 3e-13 | Disease resistance protein Roq1-like, winged-helix domain |
| LRR_14 | PF23598 | 760 - 856 | 1.4e-11 | Leucine-rich repeat region |
| LRR_14 | PF23598 | 857 - 923 | 1.1e-07 | Leucine-rich repeat region |
| LRR_14 | PF23598 | 921 - 995 | 7.4e-11 | Leucine-rich repeat region |
| LRR_14 | PF23598 | 998 - 1225 | 2.2e-13 | Leucine-rich repeat region |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 3942 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 1275, 1400 |
| Acc16I | TGCGCA | 1 cut(s) | 2999 |
| AccB1I | GGYRCC | 1 cut(s) | 2807 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1111 |
| AccI | GTMKAC | 2 cut(s) | 1573, 2989 |
| AccII | CGCG | 3 cut(s) | 147, 186, 1587 |
| AcoI | YGGCCR | 1 cut(s) | 237 |
| AcsI | RAATTY | 4 cut(s) | 1695, 1870, 3776, 4096 |
| AcuI | CTGAAG | 6 cut(s) | 70, 469, 1274, 1500, 4013, 4087 |
| AfeI | AGCGCT | 1 cut(s) | 1194 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 47, 363, 700, 1111, 2949, 3137, 3543, 3817 |
| AflIII | ACRYGT | 2 cut(s) | 2984, 3949 |
| AgeI | ACCGGT | 1 cut(s) | 2418 |
| AjnI | CCWGG | 4 cut(s) | 669, 1329, 1558, 3640 |
| Alw21I | GWGCWC | 2 cut(s) | 393, 2720 |
| Ama87I | CYCGRG | 3 cut(s) | 102, 600, 2261 |
| Aor51HI | AGCGCT | 1 cut(s) | 1194 |
| AoxI | GGCC | 6 cut(s) | 199, 237, 667, 2248, 3286, 3517 |
| ApeKI | GCWGC | 6 cut(s) | 61, 489, 875, 1849, 2915, 3561 |
| ApoI | RAATTY | 4 cut(s) | 1695, 1870, 3776, 4096 |
| AseI | ATTAAT | 1 cut(s) | 852 |
| AsiGI | ACCGGT | 1 cut(s) | 2418 |
| Asp700I | GAANNNNTTC | 4 cut(s) | 1604, 3094, 3346, 3777 |
| AspLEI | GCGC | 7 cut(s) | 66, 136, 425, 738, 1195, 2116, 3000 |
| AspS9I | GGNCC | 4 cut(s) | 181, 200, 377, 3286 |
| AsuC2I | CCSGG | 4 cut(s) | 103, 104, 584, 3516 |
| AsuHPI | GGTGA | 9 cut(s) | 106, 625, 1094, 1442, 1684, 1700, 2917, 3245, 4037 |
| AvaI | CYCGRG | 3 cut(s) | 102, 600, 2261 |
| AvaII | GGWCC | 2 cut(s) | 181, 377 |
| AxyI | CCTNAGG | 1 cut(s) | 3614 |
| BaeGI | GKGCMC | 1 cut(s) | 2703 |
| BamHI | GGATCC | 2 cut(s) | 349, 635 |
| BanI | GGYRCC | 1 cut(s) | 2807 |
| BanII | GRGCYC | 1 cut(s) | 2720 |
| BauI | CACGAG | 1 cut(s) | 2796 |
| BbsI | GAAGAC | 6 cut(s) | 102, 1385, 1398, 1488, 1531, 3598 |
| Bbv12I | GWGCWC | 2 cut(s) | 393, 2720 |
| BbvCI | CCTCAGC | 1 cut(s) | 2837 |
| BbvI | GCAGC | 6 cut(s) | 48, 501, 862, 1836, 2927, 3548 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 3191, 3225 |
| BciT130I | CCWGG | 4 cut(s) | 671, 1331, 1560, 3642 |
| BciVI | GTATCC | 2 cut(s) | 3909, 3931 |
| BclI | TGATCA | 2 cut(s) | 1504, 3907 |
| BcnI | CCSGG | 4 cut(s) | 103, 104, 584, 3516 |
| BfaI | CTAG | 8 cut(s) | 164, 1088, 1212, 1322, 1349, 1649, 3099, 3461 |
| BfmI | CTRYAG | 3 cut(s) | 1818, 2913, 3060 |
| BfoI | RGCGCY | 5 cut(s) | 137, 426, 739, 1196, 2117 |
| BfuI | GTATCC | 2 cut(s) | 3909, 3931 |
| BglII | AGATCT | 1 cut(s) | 1940 |
| BmcAI | AGTACT | 3 cut(s) | 909, 1440, 2626 |
| Bme1390I | CCNGG | 8 cut(s) | 103, 104, 584, 671, 1331, 1560, 3516, 3642 |
| Bme18I | GGWCC | 2 cut(s) | 181, 377 |
| BmeT110I | CYCGRG | 3 cut(s) | 102, 600, 2261 |
| BmgT120I | GGNCC | 4 cut(s) | 181, 200, 377, 3286 |
| BmiI | GGNNCC | 5 cut(s) | 351, 637, 2177, 2600, 2809 |
| BmrFI | CCNGG | 8 cut(s) | 103, 104, 584, 671, 1331, 1560, 3516, 3642 |
| BmrI | ACTGGG | 1 cut(s) | 1121 |
| BmsI | GCATC | 7 cut(s) | 1072, 1240, 1350, 1488, 3020, 3199, 3635 |
| BmuI | ACTGGG | 1 cut(s) | 1121 |
| BoxI | GACNNNNGTC | 1 cut(s) | 2746 |
| BpiI | GAAGAC | 6 cut(s) | 102, 1385, 1398, 1488, 1531, 3598 |
| BpmI | CTGGAG | 2 cut(s) | 1923, 2975 |
| Bpu10I | CCTNAGC | 1 cut(s) | 2837 |
| BpuEI | CTTGAG | 8 cut(s) | 256, 478, 2153, 2561, 2843, 2852, 3379, 3654 |
| BpuMI | CCSGG | 4 cut(s) | 103, 104, 584, 3516 |
| BsaAI | YACGTR | 1 cut(s) | 3952 |
| BsaI | GGTCTC | 2 cut(s) | 436, 575 |
| BsaJI | CCNNGG | 8 cut(s) | 102, 184, 257, 426, 670, 699, 1280, 3537 |
| BsaWI | WCCGGW | 2 cut(s) | 374, 2418 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1821, 1851, 2352, 2382 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 47, 363, 700, 1111, 2949, 3137, 3543, 3817 |
| Bse118I | RCCGGY | 3 cut(s) | 1129, 2418, 3894 |
| Bse1I | ACTGG | 9 cut(s) | 216, 819, 1116, 1180, 2654, 3210, 3665, 3822, 4095 |
| Bse21I | CCTNAGG | 1 cut(s) | 3614 |
| Bse3DI | GCAATG | 6 cut(s) | 1353, 1839, 2137, 2720, 3350, 3571 |
| BseBI | CCWGG | 4 cut(s) | 671, 1331, 1560, 3642 |
| BseDI | CCNNGG | 8 cut(s) | 102, 184, 257, 426, 670, 699, 1280, 3537 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 47, 363, 700, 1111, 2949, 3137, 3543, 3817 |
| BseMI | GCAATG | 6 cut(s) | 1353, 1839, 2137, 2720, 3350, 3571 |
| BseNI | ACTGG | 9 cut(s) | 216, 819, 1116, 1180, 2654, 3210, 3665, 3822, 4095 |
| BseRI | GAGGAG | 1 cut(s) | 401 |
| BseSI | GKGCMC | 1 cut(s) | 2703 |
| BseXI | GCAGC | 6 cut(s) | 48, 501, 862, 1836, 2927, 3548 |
| BseYI | CCCAGC | 1 cut(s) | 66 |
| BsgI | GTGCAG | 2 cut(s) | 2044, 2443 |
| Bsh1236I | CGCG | 3 cut(s) | 147, 186, 1587 |
| BshFI | GGCC | 6 cut(s) | 201, 239, 669, 2250, 3288, 3519 |
| BshNI | GGYRCC | 1 cut(s) | 2807 |
| BshTI | ACCGGT | 1 cut(s) | 2418 |
| BsiHKAI | GWGCWC | 2 cut(s) | 393, 2720 |
| BsiHKCI | CYCGRG | 3 cut(s) | 102, 600, 2261 |
| BsiWI | CGTACG | 1 cut(s) | 151 |
| BslFI | GGGAC | 6 cut(s) | 24, 83, 203, 603, 1718, 3175 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 47, 363, 700, 1111, 2949, 3137, 3543, 3817 |
| BsmFI | GGGAC | 6 cut(s) | 24, 83, 203, 603, 1718, 3175 |
| BsmI | GAATGC | 2 cut(s) | 3096, 3530 |
| BsnI | GGCC | 6 cut(s) | 201, 239, 669, 2250, 3288, 3519 |
| Bso31I | GGTCTC | 2 cut(s) | 436, 575 |
| BsoBI | CYCGRG | 3 cut(s) | 102, 600, 2261 |
| Bsp1286I | GDGCHC | 3 cut(s) | 393, 2703, 2720 |
| Bsp68I | TCGCGA | 1 cut(s) | 1587 |
| BspANI | GGCC | 6 cut(s) | 201, 239, 669, 2250, 3288, 3519 |
| BspFNI | CGCG | 3 cut(s) | 147, 186, 1587 |
| BspHI | TCATGA | 1 cut(s) | 1596 |
| BspLI | GGNNCC | 5 cut(s) | 351, 637, 2177, 2600, 2809 |
| BspMAI | CTGCAG | 2 cut(s) | 2917, 3064 |
| BspT107I | GGYRCC | 1 cut(s) | 2807 |
| BspTNI | GGTCTC | 2 cut(s) | 436, 575 |
| BsrDI | GCAATG | 6 cut(s) | 1353, 1839, 2137, 2720, 3350, 3571 |
| BsrFI | RCCGGY | 3 cut(s) | 1129, 2418, 3894 |
| BsrI | ACTGG | 9 cut(s) | 216, 819, 1116, 1180, 2654, 3210, 3665, 3822, 4095 |
| BssAI | RCCGGY | 3 cut(s) | 1129, 2418, 3894 |
| BssECI | CCNNGG | 8 cut(s) | 102, 184, 257, 426, 670, 699, 1280, 3537 |
| BssSI | CACGAG | 1 cut(s) | 2796 |
| BssT1I | CCWWGG | 2 cut(s) | 699, 3537 |
| Bst2BI | CACGAG | 1 cut(s) | 2796 |
| Bst2UI | CCWGG | 4 cut(s) | 671, 1331, 1560, 3642 |
| Bst6I | CTCTTC | 4 cut(s) | 603, 807, 2586, 3147 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 3043, 3570 |
| BstBAI | YACGTR | 1 cut(s) | 3952 |
| BstC8I | GCNNGC | 7 cut(s) | 294, 1565, 3002, 3316, 3571, 3638, 4070 |
| BstDSI | CCRYGG | 3 cut(s) | 184, 426, 1280 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 698, 3541 |
| BstFNI | CGCG | 3 cut(s) | 147, 186, 1587 |
| BstH2I | RGCGCY | 5 cut(s) | 137, 426, 739, 1196, 2117 |
| BstHHI | GCGC | 7 cut(s) | 66, 136, 425, 738, 1195, 2116, 3000 |
| BstNI | CCWGG | 4 cut(s) | 671, 1331, 1560, 3642 |
| BstNSI | RCATGY | 5 cut(s) | 1362, 1567, 2988, 3149, 3640 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 2746 |
| BstSCI | CCNGG | 8 cut(s) | 101, 102, 582, 669, 1329, 1558, 3514, 3640 |
| BstSFI | CTRYAG | 3 cut(s) | 1818, 2913, 3060 |
| BstSLI | GKGCMC | 1 cut(s) | 2703 |
| BstUI | CGCG | 3 cut(s) | 147, 186, 1587 |
| BstV1I | GCAGC | 6 cut(s) | 48, 501, 862, 1836, 2927, 3548 |
| BstV2I | GAAGAC | 6 cut(s) | 102, 1385, 1398, 1488, 1531, 3598 |
| BstX2I | RGATCY | 7 cut(s) | 349, 635, 1168, 1285, 1940, 2492, 3807 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 886 |
| BstYI | RGATCY | 7 cut(s) | 349, 635, 1168, 1285, 1940, 2492, 3807 |
| Bsu36I | CCTNAGG | 1 cut(s) | 3614 |
| BsuI | GTATCC | 2 cut(s) | 3909, 3931 |
| BsuRI | GGCC | 6 cut(s) | 201, 239, 669, 2250, 3288, 3519 |
| BtgI | CCRYGG | 3 cut(s) | 184, 426, 1280 |
| BtgZI | GCGATG | 2 cut(s) | 1602, 3249 |
| BtsI | GCAGTG | 4 cut(s) | 1734, 2352, 2431, 3069 |
| BtuMI | TCGCGA | 1 cut(s) | 1587 |
| Cac8I | GCNNGC | 7 cut(s) | 294, 1565, 3002, 3316, 3571, 3638, 4070 |
| CciI | TCATGA | 1 cut(s) | 1596 |
| CfoI | GCGC | 7 cut(s) | 66, 136, 425, 738, 1195, 2116, 3000 |
| Cfr10I | RCCGGY | 3 cut(s) | 1129, 2418, 3894 |
| Cfr13I | GGNCC | 4 cut(s) | 181, 200, 377, 3286 |
| Cfr42I | CCGCGG | 1 cut(s) | 187 |
| Cfr9I | CCCGGG | 1 cut(s) | 102 |
| CpoI | CGGWCCG | 1 cut(s) | 377 |
| CseI | GACGC | 2 cut(s) | 153, 1403 |
| CspAI | ACCGGT | 1 cut(s) | 2418 |
| CspI | CGGWCCG | 1 cut(s) | 377 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 1275, 1400 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 1275, 1400 |
| EaeI | YGGCCR | 1 cut(s) | 237 |
| Eam1104I | CTCTTC | 4 cut(s) | 603, 807, 2586, 3147 |
| EarI | CTCTTC | 4 cut(s) | 603, 807, 2586, 3147 |
| EciI | GGCGGA | 1 cut(s) | 20 |
| Ecl136II | GAGCTC | 1 cut(s) | 2718 |
| Eco130I | CCWWGG | 2 cut(s) | 699, 3537 |
| Eco24I | GRGCYC | 1 cut(s) | 2720 |
| Eco31I | GGTCTC | 2 cut(s) | 436, 575 |
| Eco32I | GATATC | 1 cut(s) | 631 |
| Eco47I | GGWCC | 2 cut(s) | 181, 377 |
| Eco47III | AGCGCT | 1 cut(s) | 1194 |
| Eco53kI | GAGCTC | 1 cut(s) | 2718 |
| Eco57I | CTGAAG | 6 cut(s) | 70, 469, 1274, 1500, 4013, 4087 |
| Eco81I | CCTNAGG | 1 cut(s) | 3614 |
| Eco88I | CYCGRG | 3 cut(s) | 102, 600, 2261 |
| EcoICRI | GAGCTC | 1 cut(s) | 2718 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 698, 3541 |
| EcoRII | CCWGG | 4 cut(s) | 669, 1329, 1558, 3640 |
| EcoRV | GATATC | 1 cut(s) | 631 |
| EcoT14I | CCWWGG | 2 cut(s) | 699, 3537 |
| EcoT22I | ATGCAT | 1 cut(s) | 1503 |
| EcoT38I | GRGCYC | 1 cut(s) | 2720 |
| ErhI | CCWWGG | 2 cut(s) | 699, 3537 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1328, 1360, 2396, 2428 |
| FaqI | GGGAC | 6 cut(s) | 24, 83, 203, 603, 1718, 3175 |
| FauI | CCCGC | 1 cut(s) | 179 |
| FbaI | TGATCA | 2 cut(s) | 1504, 3907 |
| FblI | GTMKAC | 2 cut(s) | 1573, 2989 |
| FriOI | GRGCYC | 1 cut(s) | 2720 |
| FspBI | CTAG | 8 cut(s) | 164, 1088, 1212, 1322, 1349, 1649, 3099, 3461 |
| FspI | TGCGCA | 1 cut(s) | 2999 |
| GlaI | GCGC | 7 cut(s) | 65, 135, 424, 737, 1194, 2115, 2999 |
| GsaI | CCCAGC | 1 cut(s) | 70 |
| GsuI | CTGGAG | 2 cut(s) | 1923, 2975 |
| HaeII | RGCGCY | 5 cut(s) | 137, 426, 739, 1196, 2117 |
| HaeIII | GGCC | 6 cut(s) | 201, 239, 669, 2250, 3288, 3519 |
| HgaI | GACGC | 2 cut(s) | 153, 1403 |
| HhaI | GCGC | 7 cut(s) | 66, 136, 425, 738, 1195, 2116, 3000 |
| Hin6I | GCGC | 7 cut(s) | 64, 134, 423, 736, 1193, 2114, 2998 |
| HinP1I | GCGC | 7 cut(s) | 64, 134, 423, 736, 1193, 2114, 2998 |
| HincII | GTYRAC | 3 cut(s) | 1743, 1807, 3261 |
| HindII | GTYRAC | 3 cut(s) | 1743, 1807, 3261 |
| HindIII | AAGCTT | 4 cut(s) | 2156, 2219, 2677, 3002 |
| HpaI | GTTAAC | 1 cut(s) | 1807 |
| HphI | GGTGA | 9 cut(s) | 106, 625, 1094, 1442, 1684, 1700, 2917, 3245, 4037 |
| Hpy166II | GTNNAC | 9 cut(s) | 33, 1280, 1574, 1743, 1807, 2194, 2990, 3261, 3333 |
| Hpy8I | GTNNAC | 9 cut(s) | 33, 1280, 1574, 1743, 1807, 2194, 2990, 3261, 3333 |
| Hpy99I | CGWCG | 3 cut(s) | 147, 362, 1009 |
| HpyCH4IV | ACGT | 3 cut(s) | 72, 360, 3951 |
| HpySE526I | ACGT | 3 cut(s) | 72, 360, 3951 |
| HspAI | GCGC | 7 cut(s) | 64, 134, 423, 736, 1193, 2114, 2998 |
| Ksp22I | TGATCA | 2 cut(s) | 1504, 3907 |
| KspAI | GTTAAC | 1 cut(s) | 1807 |
| KspI | CCGCGG | 1 cut(s) | 187 |
| LmnI | GCTCC | 4 cut(s) | 141, 388, 1829, 3988 |
| Lsp1109I | GCAGC | 6 cut(s) | 48, 501, 862, 1836, 2927, 3548 |
| LweI | GCATC | 7 cut(s) | 1072, 1240, 1350, 1488, 3020, 3199, 3635 |
| MaeI | CTAG | 8 cut(s) | 164, 1088, 1212, 1322, 1349, 1649, 3099, 3461 |
| MaeII | ACGT | 3 cut(s) | 72, 360, 3951 |
| MfeI | CAATTG | 5 cut(s) | 1398, 1726, 2733, 2874, 3903 |
| MflI | RGATCY | 7 cut(s) | 349, 635, 1168, 1285, 1940, 2492, 3807 |
| MhlI | GDGCHC | 3 cut(s) | 393, 2703, 2720 |
| MlyI | GAGTC | 6 cut(s) | 199, 221, 263, 711, 1914, 2374 |
| MmeI | TCCRAC | 5 cut(s) | 28, 163, 381, 637, 1050 |
| Mph1103I | ATGCAT | 1 cut(s) | 1503 |
| MroXI | GAANNNNTTC | 4 cut(s) | 1604, 3094, 3346, 3777 |
| MslI | CAYNNNNRTG | 1 cut(s) | 884 |
| MspA1I | CMGCKG | 4 cut(s) | 186, 866, 2977, 3581 |
| MspR9I | CCNGG | 8 cut(s) | 103, 104, 584, 671, 1331, 1560, 3516, 3642 |
| MunI | CAATTG | 5 cut(s) | 1398, 1726, 2733, 2874, 3903 |
| Mva1269I | GAATGC | 2 cut(s) | 3096, 3530 |
| MvaI | CCWGG | 4 cut(s) | 671, 1331, 1560, 3642 |
| MvnI | CGCG | 3 cut(s) | 147, 186, 1587 |
| NciI | CCSGG | 4 cut(s) | 103, 104, 584, 3516 |
| NlaIV | GGNNCC | 5 cut(s) | 351, 637, 2177, 2600, 2809 |
| NmeAIII | GCCGAG | 2 cut(s) | 209, 282 |
| NmuCI | GTSAC | 6 cut(s) | 1100, 1676, 1688, 2325, 2656, 3064 |
| NruI | TCGCGA | 1 cut(s) | 1587 |
| NsbI | TGCGCA | 1 cut(s) | 2999 |
| NsiI | ATGCAT | 1 cut(s) | 1503 |
| NspI | RCATGY | 5 cut(s) | 1362, 1567, 2988, 3149, 3640 |
| PaeI | GCATGC | 2 cut(s) | 1567, 3640 |
| PaeR7I | CTCGAG | 1 cut(s) | 2261 |
| PagI | TCATGA | 1 cut(s) | 1596 |
| PciI | ACATGT | 1 cut(s) | 2984 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 151 |
| PctI | GAATGC | 2 cut(s) | 3096, 3530 |
| PdmI | GAANNNNTTC | 4 cut(s) | 1604, 3094, 3346, 3777 |
| Pfl23II | CGTACG | 1 cut(s) | 151 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1111 |
| PinAI | ACCGGT | 1 cut(s) | 2418 |
| PleI | GAGTC | 6 cut(s) | 199, 221, 263, 711, 1913, 2373 |
| PpsI | GAGTC | 6 cut(s) | 199, 221, 263, 711, 1913, 2373 |
| Ppu21I | YACGTR | 1 cut(s) | 3952 |
| PscI | ACATGT | 1 cut(s) | 2984 |
| PshAI | GACNNNNGTC | 1 cut(s) | 2746 |
| PshBI | ATTAAT | 1 cut(s) | 852 |
| PsiI | TTATAA | 1 cut(s) | 3942 |
| Psp124BI | GAGCTC | 1 cut(s) | 2720 |
| Psp6I | CCWGG | 4 cut(s) | 669, 1329, 1558, 3640 |
| PspFI | CCCAGC | 1 cut(s) | 66 |
| PspGI | CCWGG | 4 cut(s) | 669, 1329, 1558, 3640 |
| PspLI | CGTACG | 1 cut(s) | 151 |
| PspN4I | GGNNCC | 5 cut(s) | 351, 637, 2177, 2600, 2809 |
| PspPI | GGNCC | 4 cut(s) | 181, 200, 377, 3286 |
| PspXI | VCTCGAGB | 1 cut(s) | 2261 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 900, 932 |
| PstI | CTGCAG | 2 cut(s) | 2917, 3064 |
| PsuI | RGATCY | 7 cut(s) | 349, 635, 1168, 1285, 1940, 2492, 3807 |
| PvuII | CAGCTG | 2 cut(s) | 2977, 3581 |
| RruI | TCGCGA | 1 cut(s) | 1587 |
| RseI | CAYNNNNRTG | 1 cut(s) | 884 |
| Rsr2I | CGGWCCG | 1 cut(s) | 377 |
| RsrII | CGGWCCG | 1 cut(s) | 377 |
| SacI | GAGCTC | 1 cut(s) | 2720 |
| SacII | CCGCGG | 1 cut(s) | 187 |
| Sau96I | GGNCC | 4 cut(s) | 181, 200, 377, 3286 |
| ScaI | AGTACT | 3 cut(s) | 909, 1440, 2626 |
| SchI | GAGTC | 6 cut(s) | 199, 221, 263, 711, 1914, 2374 |
| ScrFI | CCNGG | 8 cut(s) | 103, 104, 584, 671, 1331, 1560, 3516, 3642 |
| SduI | GDGCHC | 3 cut(s) | 393, 2703, 2720 |
| SfaNI | GCATC | 7 cut(s) | 1072, 1240, 1350, 1488, 3020, 3199, 3635 |
| SfcI | CTRYAG | 3 cut(s) | 1818, 2913, 3060 |
| Sfr274I | CTCGAG | 1 cut(s) | 2261 |
| Sfr303I | CCGCGG | 1 cut(s) | 187 |
| SgrBI | CCGCGG | 1 cut(s) | 187 |
| SinI | GGWCC | 2 cut(s) | 181, 377 |
| SlaI | CTCGAG | 1 cut(s) | 2261 |
| SmaI | CCCGGG | 1 cut(s) | 104 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 884 |
| SmlI | CTYRAG | 9 cut(s) | 271, 493, 2132, 2261, 2540, 2822, 2831, 3394, 3669 |
| SmoI | CTYRAG | 9 cut(s) | 271, 493, 2132, 2261, 2540, 2822, 2831, 3394, 3669 |
| SphI | GCATGC | 2 cut(s) | 1567, 3640 |
| SspMI | CTAG | 8 cut(s) | 164, 1088, 1212, 1322, 1349, 1649, 3099, 3461 |
| SstI | GAGCTC | 1 cut(s) | 2720 |
| StyD4I | CCNGG | 8 cut(s) | 101, 102, 582, 669, 1329, 1558, 3514, 3640 |
| StyI | CCWWGG | 2 cut(s) | 699, 3537 |
| TaiI | ACGT | 3 cut(s) | 75, 363, 3954 |
| TaqI | TCGA | 7 cut(s) | 222, 1028, 2168, 2206, 2262, 2400, 3252 |
| TatI | WGTACW | 4 cut(s) | 907, 1438, 2624, 3425 |
| TauI | GCSGC | 6 cut(s) | 150, 236, 239, 370, 3306, 3970 |
| TseFI | GTSAC | 6 cut(s) | 1100, 1676, 1688, 2325, 2656, 3064 |
| TseI | GCWGC | 6 cut(s) | 61, 489, 875, 1849, 2915, 3561 |
| Tsp45I | GTSAC | 6 cut(s) | 1100, 1676, 1688, 2325, 2656, 3064 |
| TspGWI | ACGGA | 2 cut(s) | 567, 981 |
| TspMI | CCCGGG | 1 cut(s) | 102 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1111 |
| VpaK11BI | GGWCC | 2 cut(s) | 181, 377 |
| VspI | ATTAAT | 1 cut(s) | 852 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 698, 3541 |
| XapI | RAATTY | 4 cut(s) | 1695, 1870, 3776, 4096 |
| XbaI | TCTAGA | 3 cut(s) | 1087, 1321, 1348 |
| XceI | RCATGY | 5 cut(s) | 1362, 1567, 2988, 3149, 3640 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 838, 2527, 2950 |
| XhoI | CTCGAG | 1 cut(s) | 2261 |
| XmaI | CCCGGG | 1 cut(s) | 102 |
| XmiI | GTMKAC | 2 cut(s) | 1573, 2989 |
| XmnI | GAANNNNTTC | 4 cut(s) | 1604, 3094, 3346, 3777 |
| XspI | CTAG | 8 cut(s) | 164, 1088, 1212, 1322, 1349, 1649, 3099, 3461 |
| ZrmI | AGTACT | 3 cut(s) | 909, 1440, 2626 |
| Zsp2I | ATGCAT | 1 cut(s) | 1503 |