MD16G1082200.v1.1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001217
Physical Location & Seq
Reverse (-)
5772871 .. 5774450
1580 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1082200.v1.1.491

Sequence Viewer

Length: 1425 bp
ATGGGGCGCGGCGAGTTTATTAGAGAATTGCCTGATTCAGTTGGAGGTTTAAATTCTCTAGTTGTGCTACAAATAAATGAGACATCAATCACAATTCTCCCACATGAAATCGGTGCCTTGAAAACACTTGAGAAGCTTGAGATGAGGAAATGTGGATTTCTTAGATCATTACCGCAGTCAATCGGAAACATGAGGGGACTAACTGCTATAGTCATAACCGATGCTACTATCACAGAGTTGCCAGAATCAGTAGGGATGTTGGAAAATCTTACCGTGTTGCACTTGAACGGATGTAAACAGTTACGCGAGCTTCCAGCTTCAATAGGGCAACTCAAGTCCTTGCACAGATTGCAAATGGCGAATACTGCAGTAACAGAACTACCTGAGAGCTTTGGAATGCTTTCTAGTTTGATGGTTTTAAAAATGGGAAAGACGCTTCAAAAGAGGGAACATACAGAAGAGATCAATTTTATACTCCCAACTTCTTTCTCAAATCTGTCCTTCCTATATGATCTGGTTGCTCATGCTTGTAATATATCTGGCAAAATTGTTGATGATTTGGAGAGGTTTGTGTCACTCGAAACCTTGAACTTAAGCCGCAATAGTTTTTGCAGCCTTCCAGCCAGTCTCAGGGGACTGTCCGTTCTTAAGAAGCTTTTGTTGTCCCATTGTAAAAAGCTCAAGTCTCTGCCTCCGCTTCCCTCGAGTTTGGATGAAGTGAATATTGCAAACTGCACCTCATTAGAAAGTATCTCTGATATTTCAAAGCTGCAAAACTTGGTGGAGCTGAACCTAGCAAGTTGTGATAAAGTTGTCGATATTCCAGGCCTTGAATGCTTGAATTCCTTGGTGAGGTTGTATGCGAGTGGTTGCAATGCTTGCTCATCAGTTGTTAAGAAAAGACTTGCTAAGAGCTACTTGAGGAAAATACGCAATCTTAGCATGCCAGGAAGCAAGATACCAGACTGGTTTTCTCAAGGTGTGGTTACATTCTCGGAGAGAAAAAACTGCGTTCTCAAAAGCGTGATCATAGGCGTTGTAGTCTCTCTCAACCAACAAATACCAGATGAAATGAGAGAAGAACTCCCTGCAATAGTGGACATTCAAGCAAAGATCCTTGTAGTGGATTTTCCTACCTTCACTTCAGCATTGAACTTACAGGGAGTACCAAACACAAATGAAGACCAATTTCACTTGTGCCGATACCCGACTGGGCATCCCTTGGTTTCTCAGTTAAAAGAAGGTTATAAGATACATGTAACGAGGAGAGATCCGCCATTGATGACAGGCGTGGAGCTGAAGAAGTGGGGGATTCGTTTTGTCTACGAAGGTGATGATGAATATGAAGGAGATGAGGAGTCTTTAAATGAAAGCCAGCAATCCTTGTCAGAAAAACTGGCCAAGTTTTTCGACTCGTTTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

475

Amino Acids

52.63

Weight (kDa)

6.2

Isoelectric Point (pI)

41.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 7 - 68 6.1e-08 Leucine-rich repeat region
LRR_14 PF23598 54 - 101 9.5e-06 Leucine-rich repeat region
LRR_14 PF23598 100 - 196 2.2e-10 Leucine-rich repeat region
LRR_13 PF23286 102 - 257 2.5e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 188 - 325 8.6e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1248
AccB1I GGYRCC 1 cut(s) 113
AccI GTMKAC 1 cut(s) 1323
AccII CGCG 2 cut(s) 9, 306
AciI CCGC 5 cut(s) 9, 173, 598, 695, 1274
AclWI GGATC 2 cut(s) 1108, 1265
AcoI YGGCCR 1 cut(s) 1398
AcsI RAATTY 2 cut(s) 52, 841
AcuI CTGAAG 2 cut(s) 1128, 1319
AfaI GTAC 1 cut(s) 1167
AfiI CCNNNNNNNGG 3 cut(s) 630, 852, 1123
AflII CTTAAG 2 cut(s) 592, 647
AflIII ACRYGT 1 cut(s) 1255
AjnI CCWGG 2 cut(s) 823, 946
AloI GAACNNNNNNTCC 2 cut(s) 627, 659
Alw26I GTCTC 4 cut(s) 74, 632, 690, 1048
AlwI GGATC 2 cut(s) 1108, 1265
Ama87I CYCGRG 1 cut(s) 703
AoxI GGCC 2 cut(s) 826, 1398
ApeKI GCWGC 2 cut(s) 612, 769
ApoI RAATTY 2 cut(s) 52, 841
Asp700I GAANNNNTTC 1 cut(s) 400
AspLEI GCGC 1 cut(s) 9
AsuHPI GGTGA 2 cut(s) 862, 1343
AvaI CYCGRG 1 cut(s) 703
BalI TGGCCA 1 cut(s) 1400
BanI GGYRCC 1 cut(s) 113
BbsI GAAGAC 1 cut(s) 1188
BbvI GCAGC 2 cut(s) 624, 756
BccI CCATC 1 cut(s) 406
BciT130I CCWGG 2 cut(s) 825, 948
BclI TGATCA 1 cut(s) 1026
BcoDI GTCTC 4 cut(s) 74, 632, 690, 1048
BfaI CTAG 3 cut(s) 59, 405, 794
BfmI CTRYAG 2 cut(s) 207, 366
BfrI CTTAAG 2 cut(s) 592, 647
BisI GCNGC 4 cut(s) 10, 598, 613, 770
BlsI GCNGC 4 cut(s) 11, 599, 614, 771
Bme1390I CCNGG 2 cut(s) 825, 948
BmeT110I CYCGRG 1 cut(s) 703
BmiI GGNNCC 1 cut(s) 115
BmrFI CCNGG 2 cut(s) 825, 948
BmrI ACTGGG 1 cut(s) 1221
BmsI GCATC 2 cut(s) 211, 1225
BmuI ACTGGG 1 cut(s) 1221
BpiI GAAGAC 1 cut(s) 1188
BplI GAGNNNNNCTC 2 cut(s) 1068, 1100
BpuEI CTTGAG 6 cut(s) 149, 158, 317, 665, 940, 960
BsaJI CCNNGG 2 cut(s) 846, 1221
Bsc4I CCNNNNNNNGG 3 cut(s) 630, 852, 1123
Bse1I ACTGG 4 cut(s) 624, 971, 1216, 1401
Bse3DI GCAATG 1 cut(s) 880
BseBI CCWGG 2 cut(s) 825, 948
BseDI CCNNGG 2 cut(s) 846, 1221
BseGI GGATG 4 cut(s) 261, 296, 718, 1216
BseLI CCNNNNNNNGG 3 cut(s) 630, 852, 1123
BseMI GCAATG 1 cut(s) 880
BseMII CTCAG 3 cut(s) 375, 643, 1244
BseNI ACTGG 4 cut(s) 624, 971, 1216, 1401
BseRI GAGGAG 2 cut(s) 1279, 1370
BseXI GCAGC 2 cut(s) 624, 756
BsgI GTGCAG 1 cut(s) 718
Bsh1236I CGCG 2 cut(s) 9, 306
BshFI GGCC 2 cut(s) 828, 1400
BshNI GGYRCC 1 cut(s) 113
BsiHKCI CYCGRG 1 cut(s) 703
BslFI GGGAC 3 cut(s) 210, 648, 649
BslI CCNNNNNNNGG 3 cut(s) 630, 852, 1123
BsmAI GTCTC 4 cut(s) 74, 632, 690, 1048
BsmFI GGGAC 3 cut(s) 210, 648, 649
BsmI GAATGC 2 cut(s) 402, 839
BsnI GGCC 2 cut(s) 828, 1400
BsoBI CYCGRG 1 cut(s) 703
Bsp143I GATC 6 cut(s) 164, 462, 511, 1026, 1113, 1270
BspACI CCGC 5 cut(s) 9, 173, 598, 695, 1274
BspANI GGCC 2 cut(s) 828, 1400
BspCNI CTCAG 3 cut(s) 376, 642, 1243
BspFNI CGCG 2 cut(s) 9, 306
BspLI GGNNCC 1 cut(s) 115
BspMAI CTGCAG 1 cut(s) 370
BspPI GGATC 2 cut(s) 1108, 1265
BspT107I GGYRCC 1 cut(s) 113
BspTI CTTAAG 2 cut(s) 592, 647
BsrDI GCAATG 1 cut(s) 880
BsrI ACTGG 4 cut(s) 624, 971, 1216, 1401
BssECI CCNNGG 2 cut(s) 846, 1221
BssMI GATC 6 cut(s) 164, 462, 511, 1026, 1113, 1270
BssT1I CCWWGG 2 cut(s) 846, 1221
Bst2UI CCWGG 2 cut(s) 825, 948
Bst4CI ACNGT 3 cut(s) 274, 300, 639
Bst6I CTCTTC 1 cut(s) 453
BstAFI CTTAAG 2 cut(s) 592, 647
BstAPI GCANNNNNTGC 2 cut(s) 349, 879
BstC8I GCNNGC 4 cut(s) 308, 880, 944, 1376
BstDEI CTNAG 6 cut(s) 161, 384, 629, 909, 938, 1230
BstENI CCTNNNNNAGG 1 cut(s) 850
BstF5I GGATG 4 cut(s) 261, 296, 718, 1216
BstFNI CGCG 2 cut(s) 9, 306
BstHHI GCGC 1 cut(s) 9
BstKTI GATC 6 cut(s) 167, 465, 514, 1029, 1116, 1273
BstMAI GTCTC 4 cut(s) 74, 632, 690, 1048
BstMBI GATC 6 cut(s) 164, 462, 511, 1026, 1113, 1270
BstMWI GCNNNNNNNGC 5 cut(s) 349, 365, 834, 879, 939
BstNI CCWGG 2 cut(s) 825, 948
BstNSI RCATGY 2 cut(s) 946, 1259
BstSCI CCNGG 2 cut(s) 823, 946
BstSFI CTRYAG 2 cut(s) 207, 366
BstUI CGCG 2 cut(s) 9, 306
BstV1I GCAGC 2 cut(s) 624, 756
BstV2I GAAGAC 1 cut(s) 1188
BstX2I RGATCY 2 cut(s) 1113, 1270
BstYI RGATCY 2 cut(s) 1113, 1270
BsuRI GGCC 2 cut(s) 828, 1400
BtsCI GGATG 4 cut(s) 261, 296, 718, 1216
Cac8I GCNNGC 4 cut(s) 308, 880, 944, 1376
CfoI GCGC 1 cut(s) 9
CseI GACGC 1 cut(s) 442
Csp6I GTAC 1 cut(s) 1166
CviAII CATG 5 cut(s) 104, 190, 524, 943, 1256
CviQI GTAC 1 cut(s) 1166
DdeI CTNAG 6 cut(s) 161, 384, 629, 909, 938, 1230
DpnI GATC 6 cut(s) 166, 464, 513, 1028, 1115, 1272
DpnII GATC 6 cut(s) 164, 462, 511, 1026, 1113, 1270
DraI TTTAAA 3 cut(s) 51, 420, 1365
EaeI YGGCCR 1 cut(s) 1398
Eam1104I CTCTTC 1 cut(s) 453
EarI CTCTTC 1 cut(s) 453
EciI GGCGGA 1 cut(s) 1263
Eco130I CCWWGG 2 cut(s) 846, 1221
Eco147I AGGCCT 1 cut(s) 828
Eco57I CTGAAG 2 cut(s) 1128, 1319
Eco88I CYCGRG 1 cut(s) 703
EcoNI CCTNNNNNAGG 1 cut(s) 850
EcoRI GAATTC 1 cut(s) 841
EcoRII CCWGG 2 cut(s) 823, 946
EcoT14I CCWWGG 2 cut(s) 846, 1221
ErhI CCWWGG 2 cut(s) 846, 1221
FaeI CATG 5 cut(s) 107, 193, 527, 946, 1259
FalI AAGNNNNNCTT 2 cut(s) 902, 934
FaqI GGGAC 3 cut(s) 210, 648, 649
FatI CATG 5 cut(s) 103, 189, 523, 942, 1255
FbaI TGATCA 1 cut(s) 1026
FblI GTMKAC 1 cut(s) 1323
Fnu4HI GCNGC 4 cut(s) 10, 598, 613, 770
FokI GGATG 4 cut(s) 268, 303, 725, 1203
Fsp4HI GCNGC 4 cut(s) 10, 598, 613, 770
FspBI CTAG 3 cut(s) 59, 405, 794
GlaI GCGC 1 cut(s) 8
GluI GCNGC 4 cut(s) 10, 598, 613, 770
HaeIII GGCC 2 cut(s) 828, 1400
HgaI GACGC 1 cut(s) 442
HhaI GCGC 1 cut(s) 9
Hin1II CATG 5 cut(s) 107, 193, 527, 946, 1259
Hin6I GCGC 1 cut(s) 7
HinP1I GCGC 1 cut(s) 7
HindIII AAGCTT 2 cut(s) 134, 653
HinfI GANTC 5 cut(s) 35, 245, 1312, 1358, 1412
HphI GGTGA 2 cut(s) 862, 1343
Hpy166II GTNNAC 3 cut(s) 296, 1099, 1324
Hpy188I TCNGA 4 cut(s) 185, 757, 997, 1390
Hpy8I GTNNAC 3 cut(s) 296, 1099, 1324
HpyAV CCTTC 6 cut(s) 511, 626, 1147, 1235, 1322, 1340
HpyCH4III ACNGT 3 cut(s) 274, 300, 639
HpyF10VI GCNNNNNNNGC 5 cut(s) 349, 365, 834, 879, 939
HpyF3I CTNAG 6 cut(s) 161, 384, 629, 909, 938, 1230
Hsp92II CATG 5 cut(s) 107, 193, 527, 946, 1259
HspAI GCGC 1 cut(s) 7
Ksp22I TGATCA 1 cut(s) 1026
Kzo9I GATC 6 cut(s) 164, 462, 511, 1026, 1113, 1270
LmnI GCTCC 2 cut(s) 784, 1294
Lsp1109I GCAGC 2 cut(s) 624, 756
LweI GCATC 2 cut(s) 211, 1225
MaeI CTAG 3 cut(s) 59, 405, 794
MaeIII GTNAC 5 cut(s) 300, 370, 573, 985, 1258
MalI GATC 6 cut(s) 166, 464, 513, 1028, 1115, 1272
MboI GATC 6 cut(s) 164, 462, 511, 1026, 1113, 1270
MboII GAAGA 4 cut(s) 470, 1091, 1193, 1312
MflI RGATCY 2 cut(s) 1113, 1270
MlsI TGGCCA 1 cut(s) 1400
MluCI AATT 7 cut(s) 26, 52, 93, 466, 546, 841, 1187
MluNI TGGCCA 1 cut(s) 1400
MlyI GAGTC 2 cut(s) 1367, 1406
MmeI TCCRAC 2 cut(s) 22, 240
Mox20I TGGCCA 1 cut(s) 1400
MroXI GAANNNNTTC 1 cut(s) 400
MscI TGGCCA 1 cut(s) 1400
MseI TTAA 7 cut(s) 50, 419, 593, 648, 894, 1235, 1364
Msp20I TGGCCA 1 cut(s) 1400
MspCI CTTAAG 2 cut(s) 592, 647
MspR9I CCNGG 2 cut(s) 825, 948
Mva1269I GAATGC 2 cut(s) 402, 839
MvaI CCWGG 2 cut(s) 825, 948
MvnI CGCG 2 cut(s) 9, 306
MwoI GCNNNNNNNGC 5 cut(s) 349, 365, 834, 879, 939
NdeII GATC 6 cut(s) 164, 462, 511, 1026, 1113, 1270
NlaIII CATG 5 cut(s) 107, 193, 527, 946, 1259
NlaIV GGNNCC 1 cut(s) 115
NmuCI GTSAC 1 cut(s) 573
NspI RCATGY 2 cut(s) 946, 1259
PaeI GCATGC 1 cut(s) 946
PaeR7I CTCGAG 1 cut(s) 703
PceI AGGCCT 1 cut(s) 828
PciI ACATGT 1 cut(s) 1255
PctI GAATGC 2 cut(s) 402, 839
PdmI GAANNNNTTC 1 cut(s) 400
PfeI GAWTC 3 cut(s) 35, 245, 1312
PkrI GCNGC 4 cut(s) 11, 599, 614, 771
PleI GAGTC 2 cut(s) 1366, 1406
PpsI GAGTC 2 cut(s) 1366, 1406
PscI ACATGT 1 cut(s) 1255
PsiI TTATAA 1 cut(s) 1248
Psp6I CCWGG 2 cut(s) 823, 946
PspGI CCWGG 2 cut(s) 823, 946
PspN4I GGNNCC 1 cut(s) 115
PspXI VCTCGAGB 1 cut(s) 703
PstI CTGCAG 1 cut(s) 370
PsuI RGATCY 2 cut(s) 1113, 1270
RsaI GTAC 1 cut(s) 1167
RsaNI GTAC 1 cut(s) 1166
SaqAI TTAA 7 cut(s) 50, 419, 593, 648, 894, 1235, 1364
SatI GCNGC 4 cut(s) 10, 598, 613, 770
Sau3AI GATC 6 cut(s) 164, 462, 511, 1026, 1113, 1270
SchI GAGTC 2 cut(s) 1367, 1406
ScrFI CCNGG 2 cut(s) 825, 948
SfaNI GCATC 2 cut(s) 211, 1225
SfcI CTRYAG 2 cut(s) 207, 366
Sfr274I CTCGAG 1 cut(s) 703
SlaI CTCGAG 1 cut(s) 703
SmlI CTYRAG 9 cut(s) 128, 137, 332, 592, 647, 680, 703, 919, 975
SmoI CTYRAG 9 cut(s) 128, 137, 332, 592, 647, 680, 703, 919, 975
SphI GCATGC 1 cut(s) 946
Sse9I AATT 7 cut(s) 26, 52, 93, 466, 546, 841, 1187
SseBI AGGCCT 1 cut(s) 828
SsiI CCGC 5 cut(s) 9, 173, 598, 695, 1274
SspI AATATT 1 cut(s) 724
SspMI CTAG 3 cut(s) 59, 405, 794
StuI AGGCCT 1 cut(s) 828
StyD4I CCNGG 2 cut(s) 823, 946
StyI CCWWGG 2 cut(s) 846, 1221
TaaI ACNGT 3 cut(s) 274, 300, 639
TaqI TCGA 4 cut(s) 579, 704, 816, 1410
TasI AATT 7 cut(s) 26, 52, 93, 466, 546, 841, 1187
TauI GCSGC 2 cut(s) 12, 600
TfiI GAWTC 3 cut(s) 35, 245, 1312
Tru1I TTAA 7 cut(s) 50, 419, 593, 648, 894, 1235, 1364
Tru9I TTAA 7 cut(s) 50, 419, 593, 648, 894, 1235, 1364
TseFI GTSAC 1 cut(s) 573
TseI GCWGC 2 cut(s) 612, 769
Tsp45I GTSAC 1 cut(s) 573
TspDTI ATGAA 7 cut(s) 120, 729, 1083, 1194, 1353, 1359, 1383
TspGWI ACGGA 2 cut(s) 303, 631
Vha464I CTTAAG 2 cut(s) 592, 647
XagI CCTNNNNNAGG 1 cut(s) 850
XapI RAATTY 2 cut(s) 52, 841
XceI RCATGY 2 cut(s) 946, 1259
XhoI CTCGAG 1 cut(s) 703
XmiI GTMKAC 1 cut(s) 1323
XmnI GAANNNNTTC 1 cut(s) 400
XspI CTAG 3 cut(s) 59, 405, 794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.