RchiOBHm_Chr4g0445031

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001217
Physical Location & Seq
Reverse (-)
65628036 .. 65633286
5251 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41265

Sequence Viewer

Length: 3735 bp
ATGACCAAAGTAGGTAATAAAGCTGGTTTGATCTCAAGAAAAGAAGCAGAGCTAATTCCAGATGAGGAAAAAAAGAAAGAAGCAGAGTTAATTGAAAGATTGGTAAGAAGGGTTATGACTGAAATAAACAAAACTCCGATGGGTCTCACTCCGTACCCGGTGGGACTTCATTCCCGAGTTGAAGAGGTGATGAAGCTGTTAGATATCGGATCCAAAACCGTTCAAGTTGTCGGAGTTCATGGCCTGGGCGGTATTGGCAAAACAACCCTTGCCAAGGCGCTTTTTAATAGACTTGTTGGTTATTTTGAGCGCCACAGTTTCATCTCAAATGTTAGAGAAATCTCAGCAGGACATGATGAGAATGGTTTAGTAACTCTTCAAAACATACTGGTTAGTGATCTTTCCAATAGGAAGATGGATAAAATTAATCAGACTACCGCTGGTATCGCTGCCATCAAAGTGGTTGTGAATGAGAAGCGAGTACTTGTTGTTCTGGATGATGTTGATAGCATAGACCAACTAAGTGCTTTAGTTGGCAACGGACAATGGTTTTATGAAGGAAGCCGAATTATTGTTACGACGAGAGATAGAGAACTCTTAACGAATCATCGTGTCAATCATAAATTGTATGAGGTCAGGGAGTTGGACGGGTCCGATGCTCTAGAACTTTTCAGTCACCATGCACTGGGAATTAGAGATATACCGGCAGATACCTTTCTGAAACTATCTAAGGAGATTGTGGATCTTACTGGTGGGCTACCGTTAGCGCTTGAAGTATTTGGCTCTAGCCTCTATGATAAGAGGACAGAAAAGGTATGGATAGATGCTCTTCATAAACTGAGACAGATTCGTCCACGGGATCTTCAGGATGTCCTGATGATAAGCTACAATGCTCTAGATGACCAGGAGAAGTGCATCTTTCTAGACATTGCATGTTTATTTGTTAAAATGAAGACCAAAAGAGAAGACGCAATTGTCATATTGAAGGGTTGTGGTTTTGATGGTGAGATAGTACTTACTGTCCTCACAGCAAGATCACTCATTAAGATCACTGAAGACACTACATTATGGATGCATGATCAAGTCAGAGACATGGGAAGACAAATCGTTACACAAGAAAACATTTCACATCCTGGCATGCGTAGTAGACTGTGGGATCGCGATGAAATCATGAATGTTTTCAAGTATGATAAGGGAACACCATCTATACAAGGGATTGTCCTAGACTTTGAAATGAAAAGGATGGTGAGTGATATAGGAGGTGACAGAATTTCTTGGGACAACTTTCGGAGAAGCCCCAATTGTACCACTGCGTTGACATACCTAAAGGAAAGATACAAAGCACATCGTAAAAGCCAAGAAGAGAGAAAGGGAGAGGTTAACATTTCCTCTACAGCTTTTGGAGCAATGGTCAATCTTAGGCTGCTTCAAATGAATAATGTGAATTTAGAAGGGAACTCAAAGTTTCTTCCTGCTGGAGTCAAGTGGTTACAATGGAAAGGTTGTCCTTTGAGATCTCTCCCTTCTGATTTCTTGCCTCCGCAACTCGCTGTCCTTGATCTTTCAGACAGCAATATCACAAGTTTGTGGGGAGGGTGCAGTATCATGGTATGCTTCAATTGTCTGACCTGCAGTGGAGACGGAAACAAGGTGGCTGAGAAATTGATGTTCTTGAATCTAAGCAACTGCATTTACCTAACTGCTATTCCTGATTTATCTGGGAATCGGGCGCTACAACAGCTTAATCTTGAGCATTGCATTTCACTGATTAAGCTTCATGGCTCGATTGGGAACCTGAACACATTAGTCCACTTGAACCTTCGAGAGTGTTCAAAGCTTGTTGAACTTCCAAGTGATGTCTCCGGCCTGAAAAAGCTCGAGTACCTAATCCTATCTGGTTGCTCACAGTTCAAAAAACTGCCAAACATGGACAGCTTGGTGTCACTGAAAGAACTTCTCCTTGATGACACTGCTATACAGAGTCTTCCCCAATCTATCTTCCGGCTTACAAAACTCGAAAAGCTCATCTTAAACCGGTGCAGTGAATTAAAAGAGCTACCTGTGGAGATTGGAAAGCTGTCTTCTTTGAAAGAAATCTCGCTTAATGGATCTGGTTTGGAAAAACTACCTAATTCCATTGGCTCTTTGGCAAACCTTGAGAAATTAAGTTTGTTCTGGTGTAAATCACTGACCACTCTTCCCAATTCCATTGGGAAGCTCAATTTCTTAGTGGAAGTTAGTACTTATGGTACTCCACTCACAGAACTGCCTGTGACCATTGGATCGTTGTCAAGCTTGAAGGACTTATCAGTTGGGCACGGTCAATTTCTGAGCTCATTGCCTGATTCAGTTGGAAGACTGCGCTCTTTAGTTGTGCTAAAGATAGAGCAGACATCAATCACAGACCTCCCACAGGAGATTGGTGAATTGAAAACACTTGAGAAACTTGAGCTGAGGAAATGTGAATCTCTTAGATCATTACCAGAGTCAATTGGAAGCATGAGGGCACTAACTTCTATAATCATAACTGCAGCAAATATCACCGAGTTGCCTGAATCCATAGGGAGGCTGGAGAATCTTACCATATTACAGCTGAACACATGTAGACAGTTCCGCAAGCTTCCAGCATCAATAGAACACTTAAAATCCTTGCACCGATTGCAGATGGTGAAAACTGCAGTGACAGAACTACCTGAAACCTTCGGAATGCTTTCTAGTTTGATGGTTTTGAGCATGGGAAAGAAGCCTCAAAATGGGAGACATGCAGAAGAGAAGTTTATACTTCCAGCTTCCTTCTCAAATCTGTCCCTGCTGTACGAACTGGATGCCTGTGCTTGTAATATATCTGGTGAAATTGCTGATGATTTCGAGAAGTTGTCATCGCTAGAAACTTTGAATCTGGGCCATAATAGTTTCTGCCGCCTTCCAGCCAGCCTGAGTGGTCTGTCCACTCTCGGAAAACTTTCATTGCCTCACTGTAAGAAGCTCAAATCTTTGCCACCGCTACCCTCAAGTTTGAAAGAGGTGGATATTGCAAACTGTACTGCATTAGAAAGTATATCTGATATATCAAACCTAGAGAACTTGACAGATTTCAACCTTACAAGCTGTGAGAAAGTGATGGATATTCCCGGCCTTGAATGCTTGGATTCCTTGGTAAGGTTGTATATGAGTGGCTGCAATGCTTGCACATCAGCTGTTAAGAGAAGACTTGCGAAAAAGAGCTACCTGAGGAAAATACGCAATCTTAGCATGCCTGGAAGCAAGATACCCGACTGGTTTTCTCAAGAAATGGTTACATTCTCAAAACGAGAAAACCGCCCCCTCAAAAGTGTAATTCTCTGTGTTGTAGTCTCGCTCAACCATCAAACACCAGACGACACAAGAGAAGAATTTCCTGCCGTGGTAGATATTCAAGCACAGATCCTCATACTGGATTCTCCTACTTTCACTACAACATTGGTCTTATCGGGAGTACCAAATACAAATGAAGATCAATTTCACTTGTGCCGGTATCCAACTGATCATCCTTTGGTATCCCAATTGAAAGATGGTTATAAAATACACGTAATGAGGAGAGAGCCGCCACATAGGAAAGGGGTGGAGCTGAAGAAGTGGGGGCTTTATTTGATTTACGAAGGTGATGATGACTATGAAGGAGATGAAGAATCATTAAATGAAAGCCAGCAATCCCTTTCAGAACAACTGGCAAATTTCTTCAGCTCTTTTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1244

Amino Acids

138.96

Weight (kDa)

6.63

Isoelectric Point (pI)

41.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 59 - 228 1.4e-19 NB-ARC domain
ATPase_2 PF01637 59 - 257 7.8e-07 ATPase domain predominantly from Archaea
NACHT PF05729 76 - 227 1.4e-06 NACHT domain
WHD_ROQ1 PF23282 301 - 371 5.1e-14 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 630 - 728 1.1e-11 Leucine-rich repeat region
LRR_14 PF23598 729 - 794 2e-07 Leucine-rich repeat region
LRR_14 PF23598 783 - 868 1.1e-12 Leucine-rich repeat region
LRR_13 PF23286 810 - 880 7.1e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 870 - 1097 4.4e-14 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3558
AasI GACNNNNNNGTC 2 cut(s) 849, 974
Acc36I ACCTGC 1 cut(s) 1637
AccB7I CCANNNNNTGG 1 cut(s) 685
AccI GTMKAC 2 cut(s) 1147, 2605
AccII CGCG 1 cut(s) 1161
AciI CCGC 8 cut(s) 249, 438, 1541, 2614, 2920, 3002, 3319, 3584
AclWI GGATC 8 cut(s) 204, 217, 750, 867, 1164, 2116, 2290, 3418
AcsI RAATTY 4 cut(s) 1269, 1444, 3392, 3712
AcuI CTGAAG 4 cut(s) 848, 1074, 3629, 3703
AfeI AGCGCT 1 cut(s) 768
AfiI CCNNNNNNNGG 7 cut(s) 274, 685, 2413, 2565, 2753, 3159, 3433
AflIII ACRYGT 2 cut(s) 2600, 3565
AgeI ACCGGT 1 cut(s) 2034
AjnI CCWGG 4 cut(s) 243, 903, 1132, 3256
Alw21I GWGCWC 1 cut(s) 2336
Alw26I GTCTC 7 cut(s) 149, 835, 1083, 1632, 1864, 2752, 3358
AlwI GGATC 8 cut(s) 204, 217, 750, 867, 1164, 2116, 2290, 3418
Ama87I CYCGRG 2 cut(s) 174, 1877
Aor51HI AGCGCT 1 cut(s) 768
AoxI GGCC 4 cut(s) 241, 1864, 2902, 3133
ApeKI GCWGC 4 cut(s) 449, 1423, 2531, 3177
ApoI RAATTY 4 cut(s) 1269, 1444, 3392, 3712
AseI ATTAAT 1 cut(s) 426
AsiGI ACCGGT 1 cut(s) 2034
Asp700I GAANNNNTTC 4 cut(s) 1178, 2710, 2962, 3393
AspLEI GCGC 5 cut(s) 280, 312, 769, 1732, 2364
AspS9I GGNCC 2 cut(s) 651, 2902
AsuC2I CCSGG 2 cut(s) 158, 3132
AvaI CYCGRG 2 cut(s) 174, 1877
AvaII GGWCC 1 cut(s) 651
AxyI CCTNAGG 1 cut(s) 3230
BaeGI GKGCMC 2 cut(s) 2319, 2509
BamHI GGATCC 1 cut(s) 209
BanII GRGCYC 1 cut(s) 2336
BbsI GAAGAC 8 cut(s) 959, 972, 1062, 1105, 1976, 2073, 2362, 3214
Bbv12I GWGCWC 1 cut(s) 2336
BbvCI CCTCAGC 1 cut(s) 2453
BbvI GCAGC 4 cut(s) 436, 1410, 2543, 3164
BceAI ACGGC 1 cut(s) 3386
BcgI CGANNNNNNTGC 2 cut(s) 2807, 2841
BciT130I CCWGG 4 cut(s) 245, 905, 1134, 3258
BciVI GTATCC 2 cut(s) 3525, 3547
BclI TGATCA 2 cut(s) 1078, 3523
BcnI CCSGG 2 cut(s) 158, 3132
BcoDI GTCTC 7 cut(s) 149, 835, 1083, 1632, 1864, 2752, 3358
BfaI CTAG 8 cut(s) 662, 786, 896, 923, 1223, 2715, 2885, 3077
BfmI CTRYAG 4 cut(s) 1392, 1630, 2529, 2676
BfoI RGCGCY 4 cut(s) 281, 313, 770, 1733
BfuAI ACCTGC 1 cut(s) 1637
BfuI GTATCC 2 cut(s) 3525, 3547
BglII AGATCT 1 cut(s) 1514
BisI GCNGC 6 cut(s) 450, 1424, 2532, 2920, 3178, 3584
BlsI GCNGC 6 cut(s) 451, 1425, 2533, 2921, 3179, 3585
BmcAI AGTACT 3 cut(s) 483, 1014, 2242
Bme1390I CCNGG 6 cut(s) 158, 245, 905, 1134, 3132, 3258
Bme18I GGWCC 1 cut(s) 651
BmeT110I CYCGRG 2 cut(s) 174, 1877
BmgT120I GGNCC 2 cut(s) 651, 2902
BmiI GGNNCC 3 cut(s) 211, 652, 1793
BmrFI CCNGG 6 cut(s) 158, 245, 905, 1134, 3132, 3258
BmrI ACTGGG 1 cut(s) 695
BmsI GCATC 6 cut(s) 646, 814, 924, 1062, 2636, 2815
BmuI ACTGGG 1 cut(s) 695
BpiI GAAGAC 8 cut(s) 959, 972, 1062, 1105, 1976, 2073, 2362, 3214
BpmI CTGGAG 2 cut(s) 1497, 2591
Bpu10I CCTNAGC 1 cut(s) 2453
BpuEI CTTGAG 7 cut(s) 19, 1769, 2177, 2459, 2468, 2995, 3270
BpuMI CCSGG 2 cut(s) 158, 3132
BsaAI YACGTR 1 cut(s) 3568
BsaI GGTCTC 1 cut(s) 149
BsaJI CCNNGG 5 cut(s) 244, 273, 854, 3153, 3402
BsaWI WCCGGW 1 cut(s) 2034
BsaXI ACNNNNNCTCC 2 cut(s) 1395, 1425
Bsc4I CCNNNNNNNGG 7 cut(s) 274, 685, 2413, 2565, 2753, 3159, 3433
Bse118I RCCGGY 3 cut(s) 703, 2034, 3510
Bse1I ACTGG 7 cut(s) 393, 690, 754, 2826, 3281, 3438, 3711
Bse21I CCTNAGG 1 cut(s) 3230
Bse3DI GCAATG 6 cut(s) 927, 1413, 1753, 2336, 2966, 3187
BseBI CCWGG 4 cut(s) 245, 905, 1134, 3258
BseDI CCNNGG 5 cut(s) 244, 273, 854, 3153, 3402
BseGI GGATG 7 cut(s) 502, 874, 1077, 1129, 1248, 2830, 3526
BseLI CCNNNNNNNGG 7 cut(s) 274, 685, 2413, 2565, 2753, 3159, 3433
BseMI GCAATG 6 cut(s) 927, 1413, 1753, 2336, 2966, 3187
BseMII CTCAG 7 cut(s) 357, 830, 1647, 2321, 2444, 2927, 3221
BseNI ACTGG 7 cut(s) 393, 690, 754, 2826, 3281, 3438, 3711
BseRI GAGGAG 1 cut(s) 3589
BseSI GKGCMC 2 cut(s) 2319, 2509
BseXI GCAGC 4 cut(s) 436, 1410, 2543, 3164
BsgI GTGCAG 2 cut(s) 1618, 2059
Bsh1236I CGCG 1 cut(s) 1161
BshFI GGCC 4 cut(s) 243, 1866, 2904, 3135
BshTI ACCGGT 1 cut(s) 2034
BsiHKAI GWGCWC 1 cut(s) 2336
BsiHKCI CYCGRG 2 cut(s) 174, 1877
BsiSI CCGG 7 cut(s) 158, 704, 1863, 2002, 2035, 3132, 3511
BslFI GGGAC 3 cut(s) 177, 1292, 2791
BslI CCNNNNNNNGG 7 cut(s) 274, 685, 2413, 2565, 2753, 3159, 3433
BsmAI GTCTC 7 cut(s) 149, 835, 1083, 1632, 1864, 2752, 3358
BsmBI CGTCTC 1 cut(s) 1632
BsmFI GGGAC 3 cut(s) 177, 1292, 2791
BsmI GAATGC 2 cut(s) 2712, 3146
BsnI GGCC 4 cut(s) 243, 1866, 2904, 3135
Bso31I GGTCTC 1 cut(s) 149
BsoBI CYCGRG 2 cut(s) 174, 1877
Bsp1286I GDGCHC 3 cut(s) 2319, 2336, 2509
Bsp68I TCGCGA 1 cut(s) 1161
BspACI CCGC 8 cut(s) 249, 438, 1541, 2614, 2920, 3002, 3319, 3584
BspANI GGCC 4 cut(s) 243, 1866, 2904, 3135
BspCNI CTCAG 7 cut(s) 356, 831, 1648, 2322, 2445, 2928, 3222
BspFNI CGCG 1 cut(s) 1161
BspHI TCATGA 1 cut(s) 1170
BspLI GGNNCC 3 cut(s) 211, 652, 1793
BspMAI CTGCAG 3 cut(s) 1634, 2533, 2680
BspMI ACCTGC 1 cut(s) 1637
BspPI GGATC 8 cut(s) 204, 217, 750, 867, 1164, 2116, 2290, 3418
BspQI GCTCTTC 1 cut(s) 834
BspTNI GGTCTC 1 cut(s) 149
BsrDI GCAATG 6 cut(s) 927, 1413, 1753, 2336, 2966, 3187
BsrFI RCCGGY 3 cut(s) 703, 2034, 3510
BsrI ACTGG 7 cut(s) 393, 690, 754, 2826, 3281, 3438, 3711
BssAI RCCGGY 3 cut(s) 703, 2034, 3510
BssECI CCNNGG 5 cut(s) 244, 273, 854, 3153, 3402
BssT1I CCWWGG 2 cut(s) 273, 3153
Bst2UI CCWGG 4 cut(s) 245, 905, 1134, 3258
Bst6I CTCTTC 6 cut(s) 177, 381, 834, 1356, 2202, 2763
BstAPI GCANNNNNTGC 2 cut(s) 2659, 3186
BstBAI YACGTR 1 cut(s) 3568
BstC8I GCNNGC 6 cut(s) 1139, 2618, 2932, 3187, 3254, 3686
BstDSI CCRYGG 2 cut(s) 854, 3402
BstENI CCTNNNNNAGG 3 cut(s) 272, 2411, 3157
BstF5I GGATG 7 cut(s) 502, 874, 1077, 1129, 1248, 2830, 3526
BstFNI CGCG 1 cut(s) 1161
BstH2I RGCGCY 4 cut(s) 281, 313, 770, 1733
BstHHI GCGC 5 cut(s) 280, 312, 769, 1732, 2364
BstMAI GTCTC 7 cut(s) 149, 835, 1083, 1632, 1864, 2752, 3358
BstMWI GCNNNNNNNGC 8 cut(s) 255, 446, 1403, 1738, 2659, 3141, 3186, 3249
BstNI CCWGG 4 cut(s) 245, 905, 1134, 3258
BstNSI RCATGY 5 cut(s) 936, 1141, 2604, 2765, 3256
BstSCI CCNGG 6 cut(s) 156, 243, 903, 1132, 3130, 3256
BstSFI CTRYAG 4 cut(s) 1392, 1630, 2529, 2676
BstSLI GKGCMC 2 cut(s) 2319, 2509
BstUI CGCG 1 cut(s) 1161
BstV1I GCAGC 4 cut(s) 436, 1410, 2543, 3164
BstV2I GAAGAC 8 cut(s) 959, 972, 1062, 1105, 1976, 2073, 2362, 3214
BstX2I RGATCY 6 cut(s) 209, 742, 859, 1514, 2108, 3423
BstXI CCANNNNNNTGG 1 cut(s) 460
BstYI RGATCY 6 cut(s) 209, 742, 859, 1514, 2108, 3423
Bsu36I CCTNAGG 1 cut(s) 3230
BsuI GTATCC 2 cut(s) 3525, 3547
BsuRI GGCC 4 cut(s) 243, 1866, 2904, 3135
BtgI CCRYGG 2 cut(s) 854, 3402
BtgZI GCGATG 2 cut(s) 1176, 2865
BtsCI GGATG 7 cut(s) 502, 874, 1077, 1129, 1248, 2830, 3526
BtsI GCAGTG 5 cut(s) 1308, 1639, 1968, 2047, 2685
BtuMI TCGCGA 1 cut(s) 1161
BveI ACCTGC 1 cut(s) 1637
Cac8I GCNNGC 6 cut(s) 1139, 2618, 2932, 3187, 3254, 3686
CciI TCATGA 1 cut(s) 1170
CfoI GCGC 5 cut(s) 280, 312, 769, 1732, 2364
Cfr10I RCCGGY 3 cut(s) 703, 2034, 3510
Cfr13I GGNCC 2 cut(s) 651, 2902
CseI GACGC 1 cut(s) 977
CspAI ACCGGT 1 cut(s) 2034
DrdI GACNNNNNNGTC 2 cut(s) 849, 974
DseDI GACNNNNNNGTC 2 cut(s) 849, 974
Eam1104I CTCTTC 6 cut(s) 177, 381, 834, 1356, 2202, 2763
EarI CTCTTC 6 cut(s) 177, 381, 834, 1356, 2202, 2763
Ecl136II GAGCTC 1 cut(s) 2334
Eco130I CCWWGG 2 cut(s) 273, 3153
Eco24I GRGCYC 1 cut(s) 2336
Eco31I GGTCTC 1 cut(s) 149
Eco32I GATATC 1 cut(s) 205
Eco47I GGWCC 1 cut(s) 651
Eco47III AGCGCT 1 cut(s) 768
Eco53kI GAGCTC 1 cut(s) 2334
Eco57I CTGAAG 4 cut(s) 848, 1074, 3629, 3703
Eco81I CCTNAGG 1 cut(s) 3230
Eco88I CYCGRG 2 cut(s) 174, 1877
EcoICRI GAGCTC 1 cut(s) 2334
EcoNI CCTNNNNNAGG 3 cut(s) 272, 2411, 3157
EcoRII CCWGG 4 cut(s) 243, 903, 1132, 3256
EcoRV GATATC 1 cut(s) 205
EcoT14I CCWWGG 2 cut(s) 273, 3153
EcoT22I ATGCAT 1 cut(s) 1077
EcoT38I GRGCYC 1 cut(s) 2336
ErhI CCWWGG 2 cut(s) 273, 3153
Esp3I CGTCTC 1 cut(s) 1632
FalI AAGNNNNNCTT 4 cut(s) 902, 934, 2012, 2044
FaqI GGGAC 3 cut(s) 177, 1292, 2791
FbaI TGATCA 2 cut(s) 1078, 3523
FblI GTMKAC 2 cut(s) 1147, 2605
Fnu4HI GCNGC 6 cut(s) 450, 1424, 2532, 2920, 3178, 3584
FokI GGATG 7 cut(s) 509, 881, 1084, 1116, 1255, 2837, 3513
FriOI GRGCYC 1 cut(s) 2336
Fsp4HI GCNGC 6 cut(s) 450, 1424, 2532, 2920, 3178, 3584
FspBI CTAG 8 cut(s) 662, 786, 896, 923, 1223, 2715, 2885, 3077
GlaI GCGC 5 cut(s) 279, 311, 768, 1731, 2363
GluI GCNGC 6 cut(s) 450, 1424, 2532, 2920, 3178, 3584
GsuI CTGGAG 2 cut(s) 1497, 2591
HaeII RGCGCY 4 cut(s) 281, 313, 770, 1733
HaeIII GGCC 4 cut(s) 243, 1866, 2904, 3135
HapII CCGG 7 cut(s) 158, 704, 1863, 2002, 2035, 3132, 3511
HgaI GACGC 1 cut(s) 977
HhaI GCGC 5 cut(s) 280, 312, 769, 1732, 2364
Hin6I GCGC 5 cut(s) 278, 310, 767, 1730, 2362
HinP1I GCGC 5 cut(s) 278, 310, 767, 1730, 2362
HincII GTYRAC 2 cut(s) 1317, 1381
HindII GTYRAC 2 cut(s) 1317, 1381
HindIII AAGCTT 4 cut(s) 1772, 1835, 2293, 2618
HpaI GTTAAC 1 cut(s) 1381
HpaII CCGG 7 cut(s) 158, 704, 1863, 2002, 2035, 3132, 3511
Hpy166II GTNNAC 7 cut(s) 854, 1148, 1317, 1381, 1810, 2606, 2949
Hpy8I GTNNAC 7 cut(s) 854, 1148, 1317, 1381, 1810, 2606, 2949
Hpy99I CGWCG 1 cut(s) 583
HpyCH4IV ACGT 1 cut(s) 3567
HpyF10VI GCNNNNNNNGC 8 cut(s) 255, 446, 1403, 1738, 2659, 3141, 3186, 3249
HpySE526I ACGT 1 cut(s) 3567
HspAI GCGC 5 cut(s) 278, 310, 767, 1730, 2362
Ksp22I TGATCA 2 cut(s) 1078, 3523
KspAI GTTAAC 1 cut(s) 1381
LguI GCTCTTC 1 cut(s) 834
LmnI GCTCC 2 cut(s) 1403, 3604
Lsp1109I GCAGC 4 cut(s) 436, 1410, 2543, 3164
LweI GCATC 6 cut(s) 646, 814, 924, 1062, 2636, 2815
MaeI CTAG 8 cut(s) 662, 786, 896, 923, 1223, 2715, 2885, 3077
MaeII ACGT 1 cut(s) 3567
MfeI CAATTG 5 cut(s) 972, 1300, 1618, 2490, 3542
MflI RGATCY 6 cut(s) 209, 742, 859, 1514, 2108, 3423
MhlI GDGCHC 3 cut(s) 2319, 2336, 2509
MlyI GAGTC 3 cut(s) 1488, 1990, 2495
MmeI TCCRAC 4 cut(s) 211, 624, 2332, 3542
Mph1103I ATGCAT 1 cut(s) 1077
MroXI GAANNNNTTC 4 cut(s) 1178, 2710, 2962, 3393
MslI CAYNNNNRTG 1 cut(s) 458
MspA1I CMGCKG 3 cut(s) 440, 2593, 3197
MspI CCGG 7 cut(s) 158, 704, 1863, 2002, 2035, 3132, 3511
MspR9I CCNGG 6 cut(s) 158, 245, 905, 1134, 3132, 3258
MunI CAATTG 5 cut(s) 972, 1300, 1618, 2490, 3542
Mva1269I GAATGC 2 cut(s) 2712, 3146
MvaI CCWGG 4 cut(s) 245, 905, 1134, 3258
MvnI CGCG 1 cut(s) 1161
MwoI GCNNNNNNNGC 8 cut(s) 255, 446, 1403, 1738, 2659, 3141, 3186, 3249
NciI CCSGG 2 cut(s) 158, 3132
NlaIV GGNNCC 3 cut(s) 211, 652, 1793
NmuCI GTSAC 5 cut(s) 674, 1262, 1941, 2272, 2680
NruI TCGCGA 1 cut(s) 1161
NsiI ATGCAT 1 cut(s) 1077
NspI RCATGY 5 cut(s) 936, 1141, 2604, 2765, 3256
PaeI GCATGC 2 cut(s) 1141, 3256
PaeR7I CTCGAG 1 cut(s) 1877
PagI TCATGA 1 cut(s) 1170
PciI ACATGT 1 cut(s) 2600
PciSI GCTCTTC 1 cut(s) 834
PctI GAATGC 2 cut(s) 2712, 3146
PdmI GAANNNNTTC 4 cut(s) 1178, 2710, 2962, 3393
PflMI CCANNNNNTGG 1 cut(s) 685
PinAI ACCGGT 1 cut(s) 2034
PkrI GCNGC 6 cut(s) 451, 1425, 2533, 2921, 3179, 3585
PleI GAGTC 3 cut(s) 1487, 1989, 2494
PpsI GAGTC 3 cut(s) 1487, 1989, 2494
Ppu21I YACGTR 1 cut(s) 3568
PscI ACATGT 1 cut(s) 2600
PshBI ATTAAT 1 cut(s) 426
PsiI TTATAA 1 cut(s) 3558
Psp124BI GAGCTC 1 cut(s) 2336
Psp6I CCWGG 4 cut(s) 243, 903, 1132, 3256
PspGI CCWGG 4 cut(s) 243, 903, 1132, 3256
PspN4I GGNNCC 3 cut(s) 211, 652, 1793
PspPI GGNCC 2 cut(s) 651, 2902
PspXI VCTCGAGB 1 cut(s) 1877
PsrI GAACNNNNNNTAC 2 cut(s) 474, 506
PstI CTGCAG 3 cut(s) 1634, 2533, 2680
PsuI RGATCY 6 cut(s) 209, 742, 859, 1514, 2108, 3423
PvuII CAGCTG 2 cut(s) 2593, 3197
RruI TCGCGA 1 cut(s) 1161
RseI CAYNNNNRTG 1 cut(s) 458
SacI GAGCTC 1 cut(s) 2336
SapI GCTCTTC 1 cut(s) 834
SatI GCNGC 6 cut(s) 450, 1424, 2532, 2920, 3178, 3584
Sau96I GGNCC 2 cut(s) 651, 2902
ScaI AGTACT 3 cut(s) 483, 1014, 2242
SchI GAGTC 3 cut(s) 1488, 1990, 2495
ScrFI CCNGG 6 cut(s) 158, 245, 905, 1134, 3132, 3258
SduI GDGCHC 3 cut(s) 2319, 2336, 2509
SfaNI GCATC 6 cut(s) 646, 814, 924, 1062, 2636, 2815
SfcI CTRYAG 4 cut(s) 1392, 1630, 2529, 2676
Sfr274I CTCGAG 1 cut(s) 1877
SinI GGWCC 1 cut(s) 651
SlaI CTCGAG 1 cut(s) 1877
SmiMI CAYNNNNRTG 1 cut(s) 458
SmlI CTYRAG 8 cut(s) 34, 1748, 1877, 2156, 2438, 2447, 3010, 3285
SmoI CTYRAG 8 cut(s) 34, 1748, 1877, 2156, 2438, 2447, 3010, 3285
SphI GCATGC 2 cut(s) 1141, 3256
SsiI CCGC 8 cut(s) 249, 438, 1541, 2614, 2920, 3002, 3319, 3584
SspMI CTAG 8 cut(s) 662, 786, 896, 923, 1223, 2715, 2885, 3077
SstI GAGCTC 1 cut(s) 2336
StyD4I CCNGG 6 cut(s) 156, 243, 903, 1132, 3130, 3256
StyI CCWWGG 2 cut(s) 273, 3153
TaiI ACGT 1 cut(s) 3570
TaqI TCGA 5 cut(s) 1784, 1822, 1878, 2016, 2868
TatI WGTACW 4 cut(s) 481, 1012, 2240, 3041
TauI GCSGC 2 cut(s) 2922, 3586
TseFI GTSAC 5 cut(s) 674, 1262, 1941, 2272, 2680
TseI GCWGC 4 cut(s) 449, 1423, 2531, 3177
Tsp45I GTSAC 5 cut(s) 674, 1262, 1941, 2272, 2680
TspGWI ACGGA 3 cut(s) 141, 555, 1656
Van91I CCANNNNNTGG 1 cut(s) 685
VpaK11BI GGWCC 1 cut(s) 651
VspI ATTAAT 1 cut(s) 426
XagI CCTNNNNNAGG 3 cut(s) 272, 2411, 3157
XapI RAATTY 4 cut(s) 1269, 1444, 3392, 3712
XbaI TCTAGA 3 cut(s) 661, 895, 922
XceI RCATGY 5 cut(s) 936, 1141, 2604, 2765, 3256
XcmI CCANNNNNNNNNTGG 4 cut(s) 412, 2143, 2566, 3548
XhoI CTCGAG 1 cut(s) 1877
XmiI GTMKAC 2 cut(s) 1147, 2605
XmnI GAANNNNTTC 4 cut(s) 1178, 2710, 2962, 3393
XspI CTAG 8 cut(s) 662, 786, 896, 923, 1223, 2715, 2885, 3077
ZrmI AGTACT 3 cut(s) 483, 1014, 2242
Zsp2I ATGCAT 1 cut(s) 1077
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.