RchiOBHm_Chr4g0445061

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001217
Physical Location & Seq
Forward (+)
65634710 .. 65640304
5595 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41266

Sequence Viewer

Length: 3846 bp
ATGGCCAAAGTAGGTGGCAAAGCTGGTTTTGTTGTCAACAGCAGCAACGAAGCAGAGAGGATTCAATCTCTAGTCGAAACGGTTTTGGCTGAAATAAACAAAATCCCGGTGGGTCTTGCAGCATACACAGTGGGTCTTGATTCCCGAATTGAAGACGTGATGAAGTTGTTAGATGTTAGATCAAGTGACGTTAGAGTTGTGGGAGTTCATGGCATGGGTGGTGTTGGCAAGACAACACTTGCCAAGGCTATTTTTAATAAACTTGTTGGTCATTTTGGGGGACACAGCTTCGTCCCAAATGTTAGAGAAGTCTCTGCAGGACCTAATGGCTTGGTATCTCTTCAAAACAAACTCATCACCGATCTTTCCCCTGGTAAGGTGCCTGTGAATGACCTTGAGACTGGTATTTCTGCGATTAAGGCACTTGTATATGATAAGCGAGTTCTTGTTGTTCTGGATGATGTTGATAATGTAAACCAACTAAGTGCTTTAGTTGGCAAAGGACAATGGTTTAATGAAGGTAGCCGAATTATCATTACCACACGAGATAGAGGACTATTACCCAGTTATCTTGTGAATTATAAGTTGTATGAGGTCAGGGAGTTGGATGTGTCCCAGGCACTACAACTGTTTAGTTACCATGCACTGAGAAGAGATAGACCCACAGATAATTTCTTGAGTCTGTCCGAGGAAATTGTGTCTCTTACAGGTGGGCTACCGTTAGCTCTTGAAGTATTTGGTTCTTTTCTATTTGATAAGAGGAGAATAGAGGAATGGACAGATGCTCTGCACAAACTGACTAAGATTCGACCGCAACATCTTCAGGATGTGTTGAAGATAAGTTATGATGCACTAGACGAGCAAGAGAAGTGTATCTTCCTAGACATTGCATGCTTATTTGTAAAAATGAATCCCAAGAGAGAAGATGCAATTGACATATTGAGGAGTTGTGGTTTTGATGGTGAGATAGCAATTGCTGACCTCACAACAAAATCACTTATTAAGATTACAGAAGACAATACACTGTGGATGCACGATCAGGTCAGAGACATGGGAAGACAAATCGTGACACACGAAAGCCTTTTGGATCCGGGCATGCGTAGTAGACTGTGGGAACATGATAAAATCATGAATGTCTTCAAGGATGATACGGGGACAAGATGTATACAAGGGATAGTCCTAGAGGACCTAGACACTGAAAGGAAAATGAATTTGCCAAGGGATCTAACCGGTGACAAAATTTCTTGGAACAATTTTAAAAATAGCCCCAATCTTACCTCTGCAGTTACATATGTAAGGGAAAGATACAAAGCATATCTTCAAAATCAAGCAGAGAAAAAGAGTTGGGTTACACTTCGCTCTAAACCCTTTGGAGCTATGGTCAATCTTAGACTGCTTCAGATGAATTATGTGAATTTGGAAGGGAGATTTGAATTTCTTCCTTCTGAATTGAAGTGGTTACAATGGAAAGGTTGTCCTTTGAAATCTCTTCCTTCTGTTCTTTTCCTTCGGCGACTTGCTGGCCTTGATCTTTCATACAGCAAAGTTGAAAGATTGTGTAGTGGTAACACGAATAAGGTGGCTGAGAAATTGATGTTCCTGAATCTAAGCGGCTGCAGTTTCCTCACTACTATTCCTGATTTATCTGGGAATCATGCCTTGGAAAAGCTTATTCTTAAGAATTGCGTTGGACTGATCAAGCTCCATGATTCGATTGGGAATTTGAATACATTAGTCCACTTGAACTTGCAAGACTGTGAAAACCTCGTTGAACTTCCAAGTGATGTCTCTGGCCTGAGAAAACTTGAGAACCTAATCCTCTCTGGTTGCTCAAAATTGAAAAGACTGCCAAAAAACATCGAAACCTTGGTATCACTGAAAGAACTTCTCCTTGATAAAACTGCTATTGAAAGTCTTCCCGAAGCTATATTCCATCTTACAAAACTTGAAAAGCTCAGCTTGAACCGATGCAGAGTTTTGAAAGGGCTACCTGAGGAGATTGGTAAGCTGTGTTCTTTGAAAGAAATCTCCCTTAATGGATCTGGTTTGGAAAAACTACCTGATTCCATTGGCTCTTTGGCAAACCTTGAGATACTAAGTTTGTTCTGGTGTGAGTCACTGACTACTATTCCTAACTCCCTTGGTGACCTTAATAACTTGATGGAATTTTTTACTTACGGTACTCCAATTGAAGAACTGCCATTGAGTTTTGGTTCATTATCAAACTTGAAGGAATTATCAGTGGGACGTGGTCAATTTCTGCGAACACTACCAGATTCCATTGGAGGACTGAAATCATTGGTTGTGTTAAAGATAGATGAGACATCAATCACAGGTCTTCCACAAGAGATAAGTGCCTTGAAAACACTTGAGAAACTTGAGCTGAGGAAATGTGAATCTCTTGGATCATTACCAGAGTCAATTGGAAGCATGTGGGCACTAACTTCTATAATCATAACTGCAGCAAATATCACCGAGTTGCCTGAATCCATAGGGAGGCTGGAAAATCTTACCATGTTACGGTTGAACACATGTAAACAGTTCCGCAAGCTTCCAGCATCAATAGGACAGTTAAAATCCTTGCACCGATTGCAGATGGTGGAAACTGCAGTGACAGAACTACCTGAAAGCTTCGGAATGCTGTCTAATTTGATGGTTTTGAGCATGGGAAAGAAGCCTCAAAATGGGAGACATACAGAAGAGAAGTTTATACTTCCAGCTTCCTTCTCAAATCTGTCCCTGCTGTACGAACTGGATGCCCGTGCTTGTAATATATCTGGTGAAATTGCTGATGATTTCGAGAAGTTGTCATCACTAGAAACTTTGAATCTTGGCCGTAATAGTTTCTGCCGCCTTCCAGCCAGCCTGAGTGGTCTGTCCACTCTCGGAAAACTTTCATTGCCTCACTGTAAGAAGCTCAAGTCTTTGCCTCCACTTCCCTCAAGTTTGAAACAGGTAGATGTTGCAAACTGTGTTGCATTAGAAAATATATCTGATGTTTCAAACCTGGAGAACTTGACAGATTTCAACCTTACAAATTGTGAGAAAGTGTTGGATATTCCCGGTATTGAATGCTTGGATTCCTTGACAAGGCTGTTTATGAGTGGCTGCAACAGTTGCTCATCAGCAGTCAAGAAAAGACTAGCAAAAAAGAGTTACCCAAGGAGAATACGGACTCTTAGCATTCCTGGAAGCAAGATACCAGACTGGTTTTCTCAAGATGTGATTACATTCTCAGAACGGAAAAACCATGTTCTCAAAGATGTGATTATAGCTGTTGTAGTTTCTCTCAACCATCAAATACCAGATGACCTACGAGAAGAGCTCCCAGCTGTGGTAGATATTGTAGCAAAGATCCTCATACTGGATTCTCCTACCTTCACTTCAACGTTGGTCTTATCAGGAGTACCAAATACAAATGAAGATCAATTTTTCTTGTGCCGGTATCCAATTTCTCATCCTTTGGTATTTCAACTGAAAGATGGTTATAAGATACATGTAGAGAGGAGAGAGCCGCCGTATGTGAAAGGAGTGGAGCTGAAGAAGTGGGGGATTCATTTGGTGTATGAAGGTGATGATGATTACGAGGGGGACGAAGAGTCATTGAAGGAAAGCCAGCAGTCCCTTTCAGAAAAATTGGCCAAGTTCTTTGGCTCCTTTGATGAAGAAGCTGACTCCACCTCCATTGTTGAACAAGTAGATTGCACCCCTGAATCTAGTCATAATGAAGATGAAGGAATGGCACAAGAGATGAGAGAAAGTGACCGCGGTTACTTCATTTCTTTCTTCCTAAAGCTCTTGTCTTTCTGTGGTTCAGTTGGTTGTGTTTCGGGGTCTGAAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1281

Amino Acids

142.64

Weight (kDa)

5.86

Isoelectric Point (pI)

38.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 49 - 217 1.3e-19 NB-ARC domain
NACHT PF05729 65 - 212 5.6e-07 NACHT domain
WHD_ROQ1 PF23282 287 - 359 1.1e-14 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 546 - 629 8.2e-06 Leucine-rich repeat region
LRR_14 PF23598 653 - 722 1.6e-07 Leucine-rich repeat region
LRR_14 PF23598 727 - 798 4.4e-08 Leucine-rich repeat region
LRR_14 PF23598 847 - 922 1.5e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 582, 3489
AccB1I GGYRCC 1 cut(s) 379
AccI GTMKAC 2 cut(s) 1105, 1165
AccII CGCG 1 cut(s) 3768
AciI CCGC 7 cut(s) 812, 1611, 2545, 2851, 3515, 3766, 3768
AclI AACGTT 1 cut(s) 3389
AclWI GGATC 6 cut(s) 1082, 1095, 1230, 2047, 2413, 3349
AcoI YGGCCR 3 cut(s) 3, 2833, 3639
AcsI RAATTY 6 cut(s) 1210, 1239, 1414, 1433, 1720, 2165
AcuI CTGAAG 3 cut(s) 806, 1382, 3560
AfaI GTAC 3 cut(s) 2182, 2747, 3408
AfiI CCNNNNNNNGG 7 cut(s) 376, 2496, 2520, 2684, 3090, 3334, 3364
AflII CTTAAG 1 cut(s) 1676
AflIII ACRYGT 2 cut(s) 2531, 3496
AgeI ACCGGT 1 cut(s) 1229
AhdI GACNNNNNGTC 1 cut(s) 3598
AjiI CACGTC 2 cut(s) 157, 2249
AjnI CCWGG 4 cut(s) 370, 615, 3006, 3187
AjuI GAANNNNNNNTTGG 2 cut(s) 1643, 1675
Alw21I GWGCWC 1 cut(s) 3327
Alw26I GTCTC 7 cut(s) 316, 392, 705, 1041, 1792, 2315, 2683
AlwI GGATC 6 cut(s) 1082, 1095, 1230, 2047, 2413, 3349
AoxI GGCC 5 cut(s) 3, 1522, 1792, 2833, 3639
ApeKI GCWGC 5 cut(s) 42, 119, 1614, 2462, 3108
ApoI RAATTY 6 cut(s) 1210, 1239, 1414, 1433, 1720, 2165
AsiGI ACCGGT 1 cut(s) 1229
Asp700I GAANNNNTTC 4 cut(s) 1136, 1437, 1914, 2893
AspS9I GGNCC 2 cut(s) 320, 1186
AsuC2I CCSGG 3 cut(s) 107, 1092, 3063
AsuHPI GGTGA 7 cut(s) 349, 974, 1244, 2156, 2464, 2792, 3584
AvaII GGWCC 2 cut(s) 320, 1186
AxyI CCTNAGG 1 cut(s) 1992
BaeGI GKGCMC 1 cut(s) 2440
BaeI ACNNNNGTAYC 2 cut(s) 1854, 1887
BalI TGGCCA 2 cut(s) 5, 3641
BamHI GGATCC 1 cut(s) 1087
BanI GGYRCC 1 cut(s) 379
BanII GRGCYC 1 cut(s) 3327
BauI CACGAG 1 cut(s) 543
BbsI GAAGAC 6 cut(s) 159, 1020, 1063, 1129, 1907, 2330
Bbv12I GWGCWC 1 cut(s) 3327
BbvCI CCTCAGC 1 cut(s) 2384
BbvI GCAGC 5 cut(s) 54, 131, 1601, 2474, 3095
BccI CCATC 7 cut(s) 953, 1941, 2155, 2590, 2647, 3303, 3476
BceAI ACGGC 2 cut(s) 2820, 3502
BcgI CGANNNNNNTGC 2 cut(s) 2738, 2772
BciT130I CCWGG 4 cut(s) 372, 617, 3008, 3189
BciVI GTATCC 1 cut(s) 3456
BclI TGATCA 1 cut(s) 1695
BcnI CCSGG 3 cut(s) 107, 1092, 3063
BcoDI GTCTC 7 cut(s) 316, 392, 705, 1041, 1792, 2315, 2683
BfaI CTAG 8 cut(s) 71, 854, 881, 1181, 1190, 2816, 3143, 3717
BfmI CTRYAG 5 cut(s) 315, 1281, 1615, 2460, 2607
BfrI CTTAAG 1 cut(s) 1676
BfuI GTATCC 1 cut(s) 3456
BisI GCNGC 8 cut(s) 43, 120, 1612, 1615, 2463, 2851, 3109, 3515
BlpI GCTNAGC 1 cut(s) 1955
BlsI GCNGC 8 cut(s) 44, 121, 1613, 1616, 2464, 2852, 3110, 3516
Bme1390I CCNGG 7 cut(s) 107, 372, 617, 1092, 3008, 3063, 3189
Bme18I GGWCC 2 cut(s) 320, 1186
BmeRI GACNNNNNGTC 1 cut(s) 3598
BmgBI CACGTC 2 cut(s) 157, 2249
BmgT120I GGNCC 2 cut(s) 320, 1186
BmiI GGNNCC 3 cut(s) 381, 1089, 3655
BmrFI CCNGG 7 cut(s) 107, 372, 617, 1092, 3008, 3063, 3189
BmrI ACTGGG 1 cut(s) 558
BmsI GCATC 7 cut(s) 772, 838, 916, 1020, 1958, 2567, 2746
BmuI ACTGGG 1 cut(s) 558
BpiI GAAGAC 6 cut(s) 159, 1020, 1063, 1129, 1907, 2330
BplI GAGNNNNNCTC 2 cut(s) 3309, 3341
BpmI CTGGAG 1 cut(s) 3029
Bpu10I CCTNAGC 1 cut(s) 2384
Bpu1102I GCTNAGC 1 cut(s) 1955
BpuEI CTTGAG 9 cut(s) 416, 697, 1826, 2108, 2390, 2399, 2903, 2926, 3201
BpuMI CCSGG 3 cut(s) 107, 1092, 3063
BsaWI WCCGGW 1 cut(s) 1229
BsaXI ACNNNNNCTCC 6 cut(s) 937, 967, 1365, 1395, 3665, 3695
Bsc4I CCNNNNNNNGG 7 cut(s) 376, 2496, 2520, 2684, 3090, 3334, 3364
Bse118I RCCGGY 2 cut(s) 1229, 3441
Bse1I ACTGG 5 cut(s) 406, 564, 2757, 3212, 3369
Bse21I CCTNAGG 1 cut(s) 1992
Bse3DI GCAATG 2 cut(s) 885, 2897
BseBI CCWGG 4 cut(s) 372, 617, 3008, 3189
BseGI GGATG 7 cut(s) 463, 613, 832, 1035, 1150, 2761, 3457
BseLI CCNNNNNNNGG 7 cut(s) 376, 2496, 2520, 2684, 3090, 3334, 3364
BseMI GCAATG 2 cut(s) 885, 2897
BseMII CTCAG 8 cut(s) 638, 1575, 1787, 1969, 1983, 2375, 2858, 3249
BseNI ACTGG 5 cut(s) 406, 564, 2757, 3212, 3369
BseRI GAGGAG 4 cut(s) 775, 958, 2009, 3520
BseSI GKGCMC 1 cut(s) 2440
BseXI GCAGC 5 cut(s) 54, 131, 1601, 2474, 3095
BseYI CCCAGC 1 cut(s) 3328
BsgI GTGCAG 1 cut(s) 773
Bsh1236I CGCG 1 cut(s) 3768
Bsh1285I CGRYCG 1 cut(s) 812
BshFI GGCC 5 cut(s) 5, 1524, 1794, 2835, 3641
BshNI GGYRCC 1 cut(s) 379
BshTI ACCGGT 1 cut(s) 1229
BsiEI CGRYCG 1 cut(s) 812
BsiHKAI GWGCWC 1 cut(s) 3327
BsiSI CCGG 5 cut(s) 107, 1091, 1230, 3063, 3442
BslFI GGGAC 8 cut(s) 278, 294, 598, 1168, 2259, 2722, 3605, 3607
BslI CCNNNNNNNGG 7 cut(s) 376, 2496, 2520, 2684, 3090, 3334, 3364
BsmAI GTCTC 7 cut(s) 316, 392, 705, 1041, 1792, 2315, 2683
BsmFI GGGAC 8 cut(s) 278, 294, 598, 1168, 2259, 2722, 3605, 3607
BsmI GAATGC 3 cut(s) 2643, 3077, 3183
BsnI GGCC 5 cut(s) 5, 1524, 1794, 2835, 3641
Bsp1286I GDGCHC 2 cut(s) 2440, 3327
Bsp1720I GCTNAGC 1 cut(s) 1955
BspACI CCGC 7 cut(s) 812, 1611, 2545, 2851, 3515, 3766, 3768
BspANI GGCC 5 cut(s) 5, 1524, 1794, 2835, 3641
BspCNI CTCAG 8 cut(s) 639, 1576, 1788, 1968, 1984, 2376, 2859, 3248
BspFNI CGCG 1 cut(s) 3768
BspHI TCATGA 1 cut(s) 1128
BspLI GGNNCC 3 cut(s) 381, 1089, 3655
BspMAI CTGCAG 5 cut(s) 319, 1285, 1619, 2464, 2611
BspPI GGATC 6 cut(s) 1082, 1095, 1230, 2047, 2413, 3349
BspQI GCTCTTC 1 cut(s) 3315
BspT107I GGYRCC 1 cut(s) 379
BspTI CTTAAG 1 cut(s) 1676
BsrDI GCAATG 2 cut(s) 885, 2897
BsrFI RCCGGY 2 cut(s) 1229, 3441
BsrI ACTGG 5 cut(s) 406, 564, 2757, 3212, 3369
BssAI RCCGGY 2 cut(s) 1229, 3441
BssNAI GTATAC 1 cut(s) 1166
BssSI CACGAG 1 cut(s) 543
BssT1I CCWWGG 6 cut(s) 243, 1217, 1659, 1866, 2140, 3161
Bst1107I GTATAC 1 cut(s) 1166
Bst2BI CACGAG 1 cut(s) 543
Bst2UI CCWGG 4 cut(s) 372, 617, 3008, 3189
Bst6I CTCTTC 6 cut(s) 345, 646, 1494, 2694, 3315, 3591
BstAFI CTTAAG 1 cut(s) 1676
BstAPI GCANNNNNTGC 2 cut(s) 2590, 3117
BstC8I GCNNGC 6 cut(s) 892, 1097, 1522, 2549, 2863, 3617
BstDSI CCRYGG 1 cut(s) 3766
BstEII GGTNACC 1 cut(s) 2144
BstENI CCTNNNNNAGG 1 cut(s) 3088
BstF5I GGATG 7 cut(s) 463, 613, 832, 1035, 1150, 2761, 3457
BstFNI CGCG 1 cut(s) 3768
BstMAI GTCTC 7 cut(s) 316, 392, 705, 1041, 1792, 2315, 2683
BstMCI CGRYCG 1 cut(s) 812
BstMWI GCNNNNNNNGC 3 cut(s) 419, 2590, 3117
BstNI CCWGG 4 cut(s) 372, 617, 3008, 3189
BstNSI RCATGY 5 cut(s) 894, 1099, 2434, 2535, 3500
BstPI GGTNACC 1 cut(s) 2144
BstSCI CCNGG 7 cut(s) 105, 370, 615, 1090, 3006, 3061, 3187
BstSFI CTRYAG 5 cut(s) 315, 1281, 1615, 2460, 2607
BstSLI GKGCMC 1 cut(s) 2440
BstUI CGCG 1 cut(s) 3768
BstV1I GCAGC 5 cut(s) 54, 131, 1601, 2474, 3095
BstV2I GAAGAC 6 cut(s) 159, 1020, 1063, 1129, 1907, 2330
BstX2I RGATCY 4 cut(s) 1087, 1222, 2039, 3354
BstYI RGATCY 4 cut(s) 1087, 1222, 2039, 3354
BstZ17I GTATAC 1 cut(s) 1166
Bsu36I CCTNAGG 1 cut(s) 1992
BsuI GTATCC 1 cut(s) 3456
BsuRI GGCC 5 cut(s) 5, 1524, 1794, 2835, 3641
BtgI CCRYGG 1 cut(s) 3766
BtrI CACGTC 2 cut(s) 157, 2249
BtsCI GGATG 7 cut(s) 463, 613, 832, 1035, 1150, 2761, 3457
BtsI GCAGTG 1 cut(s) 2616
BtsIMutI CAGTG 9 cut(s) 135, 644, 1022, 1194, 1874, 2117, 2247, 2616, 2905
Cac8I GCNNGC 6 cut(s) 892, 1097, 1522, 2549, 2863, 3617
CciI TCATGA 1 cut(s) 1128
Cfr10I RCCGGY 2 cut(s) 1229, 3441
Cfr13I GGNCC 2 cut(s) 320, 1186
Cfr42I CCGCGG 1 cut(s) 3769
Csp6I GTAC 3 cut(s) 2181, 2746, 3407
CspAI ACCGGT 1 cut(s) 1229
CspCI CAANNNNNGTGG 3 cut(s) 30, 3667, 3702
CviQI GTAC 3 cut(s) 2181, 2746, 3407
DraI TTTAAA 1 cut(s) 1258
DriI GACNNNNNGTC 1 cut(s) 3598
EaeI YGGCCR 3 cut(s) 3, 2833, 3639
Eam1104I CTCTTC 6 cut(s) 345, 646, 1494, 2694, 3315, 3591
Eam1105I GACNNNNNGTC 1 cut(s) 3598
EarI CTCTTC 6 cut(s) 345, 646, 1494, 2694, 3315, 3591
Ecl136II GAGCTC 1 cut(s) 3325
Eco130I CCWWGG 6 cut(s) 243, 1217, 1659, 1866, 2140, 3161
Eco24I GRGCYC 1 cut(s) 3327
Eco47I GGWCC 2 cut(s) 320, 1186
Eco53kI GAGCTC 1 cut(s) 3325
Eco57I CTGAAG 3 cut(s) 806, 1382, 3560
Eco81I CCTNAGG 1 cut(s) 1992
Eco91I GGTNACC 1 cut(s) 2144
EcoICRI GAGCTC 1 cut(s) 3325
EcoNI CCTNNNNNAGG 1 cut(s) 3088
EcoO109I RGGNCCY 2 cut(s) 320, 1186
EcoO65I GGTNACC 1 cut(s) 2144
EcoRII CCWGG 4 cut(s) 370, 615, 3006, 3187
EcoT14I CCWWGG 6 cut(s) 243, 1217, 1659, 1866, 2140, 3161
EcoT38I GRGCYC 1 cut(s) 3327
ErhI CCWWGG 6 cut(s) 243, 1217, 1659, 1866, 2140, 3161
FalI AAGNNNNNCTT 6 cut(s) 860, 892, 1302, 1334, 1943, 1975
FaqI GGGAC 8 cut(s) 278, 294, 598, 1168, 2259, 2722, 3605, 3607
FauNDI CATATG 1 cut(s) 1291
FbaI TGATCA 1 cut(s) 1695
FblI GTMKAC 2 cut(s) 1105, 1165
Fnu4HI GCNGC 8 cut(s) 43, 120, 1612, 1615, 2463, 2851, 3109, 3515
FokI GGATG 7 cut(s) 470, 620, 839, 1042, 1157, 2768, 3444
FriOI GRGCYC 1 cut(s) 3327
Fsp4HI GCNGC 8 cut(s) 43, 120, 1612, 1615, 2463, 2851, 3109, 3515
FspBI CTAG 8 cut(s) 71, 854, 881, 1181, 1190, 2816, 3143, 3717
GluI GCNGC 8 cut(s) 43, 120, 1612, 1615, 2463, 2851, 3109, 3515
GsaI CCCAGC 1 cut(s) 3332
GsuI CTGGAG 1 cut(s) 3029
HaeIII GGCC 5 cut(s) 5, 1524, 1794, 2835, 3641
HapII CCGG 5 cut(s) 107, 1091, 1230, 3063, 3442
HincII GTYRAC 1 cut(s) 37
HindII GTYRAC 1 cut(s) 37
HindIII AAGCTT 3 cut(s) 1667, 2549, 2629
HpaII CCGG 5 cut(s) 107, 1091, 1230, 3063, 3442
HphI GGTGA 7 cut(s) 349, 974, 1244, 2156, 2464, 2792, 3584
Hpy166II GTNNAC 7 cut(s) 37, 475, 1106, 1166, 1738, 2537, 2880
Hpy8I GTNNAC 7 cut(s) 37, 475, 1106, 1166, 1738, 2537, 2880
HpyCH4IV ACGT 4 cut(s) 156, 189, 2248, 3389
HpyF10VI GCNNNNNNNGC 3 cut(s) 419, 2590, 3117
HpySE526I ACGT 4 cut(s) 156, 189, 2248, 3389
Ksp22I TGATCA 1 cut(s) 1695
KspI CCGCGG 1 cut(s) 3769
LguI GCTCTTC 1 cut(s) 3315
LmnI GCTCC 5 cut(s) 1373, 1707, 3330, 3535, 3659
Lsp1109I GCAGC 5 cut(s) 54, 131, 1601, 2474, 3095
LweI GCATC 7 cut(s) 772, 838, 916, 1020, 1958, 2567, 2746
MaeI CTAG 8 cut(s) 71, 854, 881, 1181, 1190, 2816, 3143, 3717
MaeII ACGT 4 cut(s) 156, 189, 2248, 3389
MfeI CAATTG 4 cut(s) 930, 972, 2187, 2421
MflI RGATCY 4 cut(s) 1087, 1222, 2039, 3354
MhlI GDGCHC 2 cut(s) 2440, 3327
MlsI TGGCCA 2 cut(s) 5, 3641
MluNI TGGCCA 2 cut(s) 5, 3641
MlyI GAGTC 6 cut(s) 688, 2123, 2426, 3169, 3608, 3668
MmeI TCCRAC 3 cut(s) 585, 1669, 3033
Mox20I TGGCCA 2 cut(s) 5, 3641
MroXI GAANNNNTTC 4 cut(s) 1136, 1437, 1914, 2893
MscI TGGCCA 2 cut(s) 5, 3641
Msp20I TGGCCA 2 cut(s) 5, 3641
MspA1I CMGCKG 2 cut(s) 3332, 3768
MspCI CTTAAG 1 cut(s) 1676
MspI CCGG 5 cut(s) 107, 1091, 1230, 3063, 3442
MspR9I CCNGG 7 cut(s) 107, 372, 617, 1092, 3008, 3063, 3189
MunI CAATTG 4 cut(s) 930, 972, 2187, 2421
Mva1269I GAATGC 3 cut(s) 2643, 3077, 3183
MvaI CCWGG 4 cut(s) 372, 617, 3008, 3189
MvnI CGCG 1 cut(s) 3768
MwoI GCNNNNNNNGC 3 cut(s) 419, 2590, 3117
NciI CCSGG 3 cut(s) 107, 1092, 3063
NdeI CATATG 1 cut(s) 1291
NlaIV GGNNCC 3 cut(s) 381, 1089, 3655
NmuCI GTSAC 7 cut(s) 185, 1066, 1232, 2115, 2144, 2611, 3761
NspI RCATGY 5 cut(s) 894, 1099, 2434, 2535, 3500
PaeI GCATGC 2 cut(s) 894, 1099
PagI TCATGA 1 cut(s) 1128
PciI ACATGT 2 cut(s) 2531, 3496
PciSI GCTCTTC 1 cut(s) 3315
PcsI WCGNNNNNNNCGW 2 cut(s) 1071, 3591
PctI GAATGC 3 cut(s) 2643, 3077, 3183
PdmI GAANNNNTTC 4 cut(s) 1136, 1437, 1914, 2893
PflFI GACNNNGTC 1 cut(s) 2250
PfoI TCCNGGA 1 cut(s) 3187
PinAI ACCGGT 1 cut(s) 1229
PkrI GCNGC 8 cut(s) 44, 121, 1613, 1616, 2464, 2852, 3110, 3516
PleI GAGTC 6 cut(s) 687, 2122, 2425, 3169, 3607, 3668
PpsI GAGTC 6 cut(s) 687, 2122, 2425, 3169, 3607, 3668
PpuMI RGGWCCY 2 cut(s) 320, 1186
PscI ACATGT 2 cut(s) 2531, 3496
PsiI TTATAA 2 cut(s) 582, 3489
Psp124BI GAGCTC 1 cut(s) 3327
Psp1406I AACGTT 1 cut(s) 3389
Psp5II RGGWCCY 2 cut(s) 320, 1186
Psp6I CCWGG 4 cut(s) 370, 615, 3006, 3187
PspEI GGTNACC 1 cut(s) 2144
PspFI CCCAGC 1 cut(s) 3328
PspGI CCWGG 4 cut(s) 370, 615, 3006, 3187
PspN4I GGNNCC 3 cut(s) 381, 1089, 3655
PspPI GGNCC 2 cut(s) 320, 1186
PspPPI RGGWCCY 2 cut(s) 320, 1186
PsrI GAACNNNNNNTAC 2 cut(s) 1996, 2028
PstI CTGCAG 5 cut(s) 319, 1285, 1619, 2464, 2611
PsuI RGATCY 4 cut(s) 1087, 1222, 2039, 3354
PsyI GACNNNGTC 1 cut(s) 2250
PvuII CAGCTG 1 cut(s) 3332
RsaI GTAC 3 cut(s) 2182, 2747, 3408
RsaNI GTAC 3 cut(s) 2181, 2746, 3407
SacI GAGCTC 1 cut(s) 3327
SacII CCGCGG 1 cut(s) 3769
SapI GCTCTTC 1 cut(s) 3315
SatI GCNGC 8 cut(s) 43, 120, 1612, 1615, 2463, 2851, 3109, 3515
Sau96I GGNCC 2 cut(s) 320, 1186
SchI GAGTC 6 cut(s) 688, 2123, 2426, 3169, 3608, 3668
ScrFI CCNGG 7 cut(s) 107, 372, 617, 1092, 3008, 3063, 3189
SduI GDGCHC 2 cut(s) 2440, 3327
SfaNI GCATC 7 cut(s) 772, 838, 916, 1020, 1958, 2567, 2746
SfcI CTRYAG 5 cut(s) 315, 1281, 1615, 2460, 2607
Sfr303I CCGCGG 1 cut(s) 3769
SgrBI CCGCGG 1 cut(s) 3769
SinI GGWCC 2 cut(s) 320, 1186
SphI GCATGC 2 cut(s) 894, 1099
SsiI CCGC 7 cut(s) 812, 1611, 2545, 2851, 3515, 3766, 3768
SspMI CTAG 8 cut(s) 71, 854, 881, 1181, 1190, 2816, 3143, 3717
SstI GAGCTC 1 cut(s) 3327
StyD4I CCNGG 7 cut(s) 105, 370, 615, 1090, 3006, 3061, 3187
StyI CCWWGG 6 cut(s) 243, 1217, 1659, 1866, 2140, 3161
TaiI ACGT 4 cut(s) 159, 192, 2251, 3392
TaqI TCGA 5 cut(s) 75, 808, 1712, 1860, 2799
TauI GCSGC 3 cut(s) 1614, 2853, 3517
TscAI CASTG 9 cut(s) 135, 651, 1029, 1201, 1881, 2124, 2247, 2616, 2912
TseFI GTSAC 7 cut(s) 185, 1066, 1232, 2115, 2144, 2611, 3761
TseI GCWGC 5 cut(s) 42, 119, 1614, 2462, 3108
Tsp45I GTSAC 7 cut(s) 185, 1066, 1232, 2115, 2144, 2611, 3761
TspGWI ACGGA 2 cut(s) 3187, 3256
TspRI CASTG 9 cut(s) 135, 651, 1029, 1201, 1881, 2124, 2247, 2616, 2912
Tth111I GACNNNGTC 1 cut(s) 2250
Vha464I CTTAAG 1 cut(s) 1676
VpaK11BI GGWCC 2 cut(s) 320, 1186
XagI CCTNNNNNAGG 1 cut(s) 3088
XapI RAATTY 6 cut(s) 1210, 1239, 1414, 1433, 1720, 2165
XceI RCATGY 5 cut(s) 894, 1099, 2434, 2535, 3500
XcmI CCANNNNNNNNNTGG 3 cut(s) 1712, 2074, 2497
XmiI GTMKAC 2 cut(s) 1105, 1165
XmnI GAANNNNTTC 4 cut(s) 1136, 1437, 1914, 2893
XspI CTAG 8 cut(s) 71, 854, 881, 1181, 1190, 2816, 3143, 3717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.