FvH4_4g35411

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001217
Physical Location & Seq
Reverse (-)
32733673 .. 32740625
6953 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g35411.t2

Sequence Viewer

Length: 4296 bp
ATGCCGTATGACGGCGCCGTCGGCGGTTTCCGGCTCCGGTGGGACGTGTTCCTGAGCTTCAGAGGCGAAGACACCCGCCACACCATCACCAACTCACTCTACGACGCGCTTGTGCAGCGCGGCGTGCGCGTCTTTCGCGACGACGACGCCCTCAACCGCGGCGACGAGATCGCGCCGAGTCTTCTGGAAGCGATCGACGACTCCGCGGCCGCCGTCGTCGTGCTCTCGCCGAGGTACGCCGACTCCAGGTGGTGCCTTGAGGAGCTCGCGAAGATCTGCCACGGCGGCCGGAGACTGATTCTGCCGGTGTTTTACCAGGTGGACCCCTCCGACGTCCGGCGACAGAGCGGGCCGTTTGAGAAGGACTTCAGGTCTCATAAGGAGAGATTTGAAAGCTCCAAGGTCTGTGAATGGAGAAAGGCCATGGCCAAAGTTGGTGGCATAGCTGGGTTTGTTTACGACAGCAGCAAAGAAGCAGAGTTGATTCAATCTTTAGTCAAAACGGTGTTGGCTGAAATAAACAGAACCCCGGTGGGTCTTGCAGCATACACAGTGGGTCTTGATTCCCGAATTGAAGACATGATGAGGTTGTTAGATGTTAGATCAAGTGACGTTCGGGTTGTGGGAGTTCATGGCATGGGTGGTGTTGGCAAGACAACACTTGCCACGGCTATTTTTAATAGACTTGTTGGTCAATTTGATGGACACAGTTTCATCTCAAATGTTAGAGAAGTCTCCGCAGGAGATAAGGGCTTGGTATCTCTTCAAAACAAACTTATCAACAATCTTTCCCCTGGTAAGATGCCTGTGACTGACATTGAGACCGGTATTTCTGCGATCAAGGCACTAGTATATGACAAGCGAGTTCTTGTTGTTCTGGATGATGTTGATAATGTTAACCAGCTAAGTGCTTCAGTTGGCAAAGGAGAATGGTTCAATGAAGGAAGCCGAATTATCATTACCACGAGGGATAAAGGACTATTACCCAGTTATCTTGTGAATTATAAGTCGTATGAGGTCAGGGAGTTGCATGAGTCCCAGGCACTACAACTGTTTAGTTACCATGCACTGAGAAGAGATAAACCCACAGGAAATTTCTTGGATCTCTCTAAAGAAATTGTGTCTCTTACAGGAGGGCTACCGTTAGCTCTTGAAGTATTTGGTTCTTTTCTATTTGATAAGAGGAGAATAGAGGAATGGACAGATGCTCTGCACAAACTGAAAAAGATTCGGCCACAACATCTTCAGGATGTGTTGAAGATAAGTTATGACGCGCTAGACGAGCAAGAAAAGTGTATCTTCTTGGATATTGCATGCTTACTTGTAAAAATGAATCCCAAGAGAGAAGATGCAATTGACATATTGAGGAGCTGTGGTTTTGATGGTGAGATAGCAATTGCTGACCTCACTGCAAAATCACTTGTTAAGATTACAGAAGACACTAAATTGTGGATGCACGATCAAGTTAGGGACATGGGAAGACAAATCGTGATACACGAAAACCTTTTGGATCCTGGCATGCGTAGTAGACTGTGGGAACATGATAAAATTATTAATGTCTTAAAGGATGATAAGGGGACAAGATGTATACAAGGCATAGTCCTAGAGGACCTAGACACTGAATGGAAATTGAAGGGTCCAAGGGATCTAACCGGCGACAAAATTTCTTGGAACAACTTTCAAAGTAGCCCCAATTTTACCTCTGCTACTACATACTTAAAGGAAAGATACAAAGCATATCTTCAAAAGCAAGCAGAGAAAAAGAGTCGGATTACAATTCACTCTAAACCCTTTGGAGCTATGGTCAATCTTAGGCTACTTCAGATGAATTATGTGAATTTGGAAGGGAGCTTCAAATTTCTTCCTTCTGAATTGAAGTGGTTACAATGGAAAGGTTGTCCTCTGAAATCTCTTCCTTCTGTTCTTTTCCTTCAGCAACTTGCTGGCCTTGATCTTTCAGAGAGCAAAGTGGAAAGATTGTGCAGTGGTAACAAGAATAAGGTGGCTGAGAAATTGATGTTCCTGAATCTAAGTGGCTGCAGTTCTCTAACTGCTATTCCTGATTTATCTGGGAATCATGCCTTGGAAAAGCTTATTCTTAAGTATTGTGTTGGACTGATTAAGCTCCATGATTCGATTGGGAATTTGAATACATTAGTCTACTTGAACTTGCAAGGGTGTATAAACCTGGTTGAACTTCCAAGTGATGTCTCCGGCCTGAGAAAACTTGAGAACCTAAACCTTTATGGTTGCTCACAATTGAAAAGACTGCCAAAAAACATCGGAAGCATGGTATCACTAAAAGAATTTGTCCTTGATGGAACTTCTATTGAAAGTCTACCCGAAACTATATTCCATCTCACAAAACTTGAAAAGCTCATCTTGAACCGGTGCGGAGCCTTGAAAGGGTTACCTGAGGAGATTGGTAAGCTGTGTTCTTTGAAGGAGATCTCCCTTAATGCATCCGGTTTGGAAAAGCTACCTGATTCCATTGGCTCTTTGGCAAACCTCGAGATACTAAGTTTGTTCTGGTGTAGTTCACTGACCACTATTCCTAACTCCCTTGGTAACCTCAATAACTTAATGGAATTTTTTACTTACGGTACTCCAATTGAAGAACTGCCATTGAGTTTTGGTTTATTATCAAACTTGAAGGAATTATCAGTAGGACATGGTCATTTTCTGCAAGCACTACCAGATTCCATTGGAGGACTAAAATCACTGGTTGTGTTAAAGATAGATGAGACATCAATCACAGGTCTTCCACAAGAGATTAGTGCCTTGAAAACACTTGAGAAGCTTGAGCTAAGGAAATGTAAATTTCTTAGATCATTACCAGAATCAATTGGAAGCCTGAGGGCACTAACTTCTATAATCATTACTGCAGCAGATATCACTGAGTTGCCTGAATCCATAGGGATGCTGGAAAATCTTACCATGTTACAGTTGAACGGGTGTAAACAGTTCCGCAAGCTCCCAACATCAATAGGACAGTTAAAGTCCTTGCACCGATTGCAGATGAGGGAAACAGCAGTGACAGAACTACCTGAAAGCTTCGGAATGCTTTCTAGTTTGATGGTTTTGAGTATGGGGAAGAAGCCTCAAAATGGGAGACATGTGGAAGAGAAGTTTATACTTCCAGCTTCCTTCTCAAATCTGTCCCTGCTGTATGAACTGGATGCTCGTGCTTGTAATATATCTGGTGAAATTTCTGATGATTTCGAGAAGTTATCATCACTTGAAACTTTGAATCTCAGCCGTAATAGTTTCTGCCGCCTTCCAGCCAGCCTGAGTGCCATGTCCGTGCTCCGAGAACTTTTATTGCCTCATTGTAGAAAGCTCAAGTCTCTGCCTCCGCTTCCCTCAAGTTTGAAAAAGGTGGATATTGCAAACTGTATTGCATTAGAAAGTATCTCTGATGTTTCAAACCTGGAGAACTTGACTGAACTGAACCTTACAAATTGTGAGAAAGTTGAGGATATTCCAGGGCTGGAATGCTTGAATTCCTTGGTAAGGTTGTACATGAGTGGCTGCAAGGCTTGCTCATCAGCTGTTAAGAGAAGACTTGCGAAAAAGAGCTACCTGAGGAAAACATGCAATCTTAGCATACCTGGAAGCAAGATACCAGACTGGTTTTCTCAAGAAATGGTTACATTCTCAAAACGAGGAAACCGCCCTCTCAAAAGTGTAATTCTATGTGTTGTGGTTTCTCTCAACCATCAAATACCTGATGACCTAAGAGAAGAACTCCCTGCTGTGGTAGATATTCAAGCACAGATCCTCATACTGGATTCTCCTACTTTCACTACAACATTGATCTTATCGGGAGTACCCAATACAAATGAGGATCAATTTCACTTGTGCCGGTATCCAATTGGTCATCCTTTGGTGTCCCAGTTGAAAGATGGTTATAGGATACATGTAATGAGGAGAGAGCCGCCATATGTGAAAGGGGTGGAGCTGAAGAAGTGGGGGATTCATTTGGTGTATGAAGGTGATGATGATTACGAGGGGGACGAAGAGTCACTGAAAGAAAGCCATCAATCCCTTTCAGAAAAACTGGCCAAGTTCTTCGGCTCTTTTGATGATGAAGAAGCTGACTTCACCTCCGGTATTGAACAAGATGATTGCATTTCTGCAAATAGAGATGGTGAAGATGAAGGAATGCCAATGGCACAAGAAATTAGAGAAAGTGAACAAAGAATATGGTTACATGATCGCCATTACTTCGTTTCTTTCTTCCTAGTGCTTTCTCTCGTCTTTCTATCTTTGAGTTGGTTGTATTTGTGGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1432

Amino Acids

160.34

Weight (kDa)

6.25

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 14 - 174 1.5e-41 TIR domain
TIR_2 PF13676 16 - 110 1.1e-15 TIR domain
NB-ARC PF00931 201 - 359 1.8e-20 NB-ARC domain
NACHT PF05729 206 - 353 6.6e-08 NACHT domain
WHD_ROQ1 PF23282 428 - 497 3.2e-13 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 675 - 769 7.4e-07 Leucine-rich repeat region
LRR_14 PF23598 750 - 844 2.2e-11 Leucine-rich repeat region
LRR_14 PF23598 801 - 863 1.4e-07 Leucine-rich repeat region
LRR_14 PF23598 868 - 938 1.8e-07 Leucine-rich repeat region
LRR_14 PF23598 911 - 984 2.9e-10 Leucine-rich repeat region
LRR_14 PF23598 988 - 1182 5.7e-14 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1005
AasI GACNNNNNNGTC 2 cut(s) 17, 690
AatII GACGTC 1 cut(s) 336
AccB1I GGYRCC 2 cut(s) 14, 252
AccBSI CCGCTC 1 cut(s) 348
AccI GTMKAC 4 cut(s) 1528, 1588, 2160, 2338
AccII CGCG 9 cut(s) 107, 120, 129, 138, 159, 173, 206, 269, 1274
AclWI GGATC 6 cut(s) 1110, 1505, 1518, 1653, 3772, 3855
AcoI YGGCCR 5 cut(s) 207, 286, 426, 1232, 4062
AcuI CTGAAG 7 cut(s) 43, 352, 897, 1229, 1805, 1916, 3983
AcyI GRCGYC 3 cut(s) 15, 147, 333
AfaI GTAC 4 cut(s) 236, 2605, 3521, 3831
AfiI CCNNNNNNNGG 8 cut(s) 11, 246, 336, 2406, 3107, 3513, 3757, 3787
AflII CTTAAG 1 cut(s) 2099
AflIII ACRYGT 3 cut(s) 45, 3115, 3919
AgeI ACCGGT 2 cut(s) 824, 2388
AhdI GACNNNNNGTC 2 cut(s) 370, 4021
AhlI ACTAGT 1 cut(s) 847
AjiI CACGTC 1 cut(s) 46
AjnI CCWGG 9 cut(s) 245, 315, 793, 1038, 1513, 2187, 3429, 3484, 3610
AjuI GAANNNNNNNTTGG 2 cut(s) 2066, 2098
Alw21I GWGCWC 3 cut(s) 225, 267, 3309
Alw26I GTCTC 9 cut(s) 286, 378, 739, 815, 1128, 2215, 2738, 3106, 3351
AlwI GGATC 6 cut(s) 1110, 1505, 1518, 1653, 3772, 3855
Ama87I CYCGRG 1 cut(s) 2510
AoxI GGCC 9 cut(s) 207, 286, 350, 420, 426, 1232, 1945, 2215, 4062
ApeKI GCWGC 6 cut(s) 115, 465, 542, 2037, 2885, 3531
AseI ATTAAT 1 cut(s) 1554
AsiGI ACCGGT 2 cut(s) 824, 2388
Asp700I GAANNNNTTC 2 cut(s) 365, 3064
AspLEI GCGC 6 cut(s) 17, 109, 120, 129, 175, 1276
AspS9I GGNCC 4 cut(s) 322, 350, 1609, 1637
AsuC2I CCSGG 1 cut(s) 530
AsuHPI GGTGA 6 cut(s) 79, 1397, 3215, 4007, 4096, 4163
AvaI CYCGRG 1 cut(s) 2510
AvaII GGWCC 3 cut(s) 322, 1609, 1637
AxyI CCTNAGG 3 cut(s) 2415, 2855, 3584
BaeGI GKGCMC 1 cut(s) 2863
BalI TGGCCA 2 cut(s) 428, 4064
BamHI GGATCC 1 cut(s) 1510
BanI GGYRCC 2 cut(s) 14, 252
BanII GRGCYC 1 cut(s) 267
BauI CACGAG 2 cut(s) 964, 3183
BbsI GAAGAC 7 cut(s) 75, 173, 582, 1443, 1486, 2753, 3568
Bbv12I GWGCWC 3 cut(s) 225, 267, 3309
BbvI GCAGC 6 cut(s) 127, 477, 554, 2024, 2897, 3518
BceAI ACGGC 7 cut(s) 2, 28, 197, 298, 337, 684, 3243
BciT130I CCWGG 9 cut(s) 247, 317, 795, 1040, 1515, 2189, 3431, 3486, 3612
BciVI GTATCC 2 cut(s) 3879, 3909
BcnI CCSGG 1 cut(s) 530
BcoDI GTCTC 9 cut(s) 286, 378, 739, 815, 1128, 2215, 2738, 3106, 3351
BcuI ACTAGT 1 cut(s) 847
BfaI CTAG 6 cut(s) 848, 1277, 1604, 1613, 3069, 4244
BfmI CTRYAG 2 cut(s) 2038, 2883
BfoI RGCGCY 1 cut(s) 18
BfrI CTTAAG 1 cut(s) 2099
BfuI GTATCC 2 cut(s) 3879, 3909
BglI GCCNNNNNGGC 1 cut(s) 285
BglII AGATCT 2 cut(s) 273, 2448
Bme18I GGWCC 3 cut(s) 322, 1609, 1637
BmeRI GACNNNNNGTC 2 cut(s) 370, 4021
BmeT110I CYCGRG 1 cut(s) 2510
BmgBI CACGTC 1 cut(s) 46
BmgT120I GGNCC 4 cut(s) 322, 350, 1609, 1637
BmiI GGNNCC 7 cut(s) 16, 35, 254, 324, 1512, 1638, 2398
BmrI ACTGGG 2 cut(s) 981, 3889
BmsI GCATC 7 cut(s) 792, 1195, 1339, 1443, 2471, 2910, 3169
BmuI ACTGGG 2 cut(s) 981, 3889
BpiI GAAGAC 7 cut(s) 75, 173, 582, 1443, 1486, 2753, 3568
BplI GAGNNNNNCTC 2 cut(s) 3732, 3764
BpmI CTGGAG 2 cut(s) 229, 3452
Bpu10I CCTNAGC 2 cut(s) 53, 2807
BpuEI CTTGAG 7 cut(s) 278, 2249, 2813, 2822, 3326, 3349, 3624
BpuMI CCSGG 1 cut(s) 530
BsaHI GRCGYC 3 cut(s) 15, 147, 333
BsaI GGTCTC 2 cut(s) 378, 815
BsaWI WCCGGW 5 cut(s) 36, 824, 2388, 2465, 4109
BsaXI ACNNNNNCTCC 8 cut(s) 227, 257, 1360, 1390, 1788, 1818, 4091, 4121
Bsc4I CCNNNNNNNGG 8 cut(s) 11, 246, 336, 2406, 3107, 3513, 3757, 3787
Bse118I RCCGGY 5 cut(s) 304, 824, 1652, 2388, 3864
Bse1I ACTGG 7 cut(s) 987, 2727, 3180, 3635, 3792, 3895, 4065
Bse21I CCTNAGG 3 cut(s) 2415, 2855, 3584
BseBI CCWGG 9 cut(s) 247, 317, 795, 1040, 1515, 2189, 3431, 3486, 3612
BseGI GGATG 8 cut(s) 886, 1255, 1458, 1573, 2462, 2925, 3184, 3880
BseLI CCNNNNNNNGG 8 cut(s) 11, 246, 336, 2406, 3107, 3513, 3757, 3787
BseNI ACTGG 7 cut(s) 987, 2727, 3180, 3635, 3792, 3895, 4065
BseRI GAGGAG 5 cut(s) 275, 1198, 1381, 2432, 3943
BseSI GKGCMC 1 cut(s) 2863
BseX3I CGGCCG 2 cut(s) 207, 286
BseXI GCAGC 6 cut(s) 127, 477, 554, 2024, 2897, 3518
BseYI CCCAGC 1 cut(s) 446
BsgI GTGCAG 3 cut(s) 134, 1196, 2002
Bsh1236I CGCG 9 cut(s) 107, 120, 129, 138, 159, 173, 206, 269, 1274
Bsh1285I CGRYCG 3 cut(s) 195, 210, 289
BshFI GGCC 9 cut(s) 209, 288, 352, 422, 428, 1234, 1947, 2217, 4064
BshNI GGYRCC 2 cut(s) 14, 252
BshTI ACCGGT 2 cut(s) 824, 2388
BsiEI CGRYCG 3 cut(s) 195, 210, 289
BsiHKAI GWGCWC 3 cut(s) 225, 267, 3309
BsiHKCI CYCGRG 1 cut(s) 2510
BslFI GGGAC 7 cut(s) 56, 1021, 1484, 1591, 3145, 3877, 4028
BslI CCNNNNNNNGG 8 cut(s) 11, 246, 336, 2406, 3107, 3513, 3757, 3787
BsmAI GTCTC 9 cut(s) 286, 378, 739, 815, 1128, 2215, 2738, 3106, 3351
BsmFI GGGAC 7 cut(s) 56, 1021, 1484, 1591, 3145, 3877, 4028
BsmI GAATGC 3 cut(s) 3066, 3500, 4170
BsnI GGCC 9 cut(s) 209, 288, 352, 422, 428, 1234, 1947, 2217, 4064
Bso31I GGTCTC 2 cut(s) 378, 815
BsoBI CYCGRG 1 cut(s) 2510
Bsp1286I GDGCHC 4 cut(s) 225, 267, 2863, 3309
Bsp1407I TGTACA 1 cut(s) 3519
Bsp19I CCATGG 1 cut(s) 423
Bsp68I TCGCGA 2 cut(s) 138, 269
BspANI GGCC 9 cut(s) 209, 288, 352, 422, 428, 1234, 1947, 2217, 4064
BspFNI CGCG 9 cut(s) 107, 120, 129, 138, 159, 173, 206, 269, 1274
BspLI GGNNCC 7 cut(s) 16, 35, 254, 324, 1512, 1638, 2398
BspMAI CTGCAG 2 cut(s) 2042, 2887
BspPI GGATC 6 cut(s) 1110, 1505, 1518, 1653, 3772, 3855
BspT107I GGYRCC 2 cut(s) 14, 252
BspTI CTTAAG 1 cut(s) 2099
BspTNI GGTCTC 2 cut(s) 378, 815
BsrBI CCGCTC 1 cut(s) 348
BsrFI RCCGGY 5 cut(s) 304, 824, 1652, 2388, 3864
BsrGI TGTACA 1 cut(s) 3519
BsrI ACTGG 7 cut(s) 987, 2727, 3180, 3635, 3792, 3895, 4065
BssAI RCCGGY 5 cut(s) 304, 824, 1652, 2388, 3864
BssNAI GTATAC 1 cut(s) 1589
BssNI GRCGYC 3 cut(s) 15, 147, 333
BssSI CACGAG 2 cut(s) 964, 3183
BssT1I CCWWGG 6 cut(s) 399, 423, 1640, 2082, 2563, 3507
Bst1107I GTATAC 1 cut(s) 1589
Bst2BI CACGAG 2 cut(s) 964, 3183
Bst2UI CCWGG 9 cut(s) 247, 317, 795, 1040, 1515, 2189, 3431, 3486, 3612
Bst6I CTCTTC 5 cut(s) 768, 1069, 1917, 3117, 4014
BstACI GRCGYC 3 cut(s) 15, 147, 333
BstAFI CTTAAG 1 cut(s) 2099
BstAPI GCANNNNNTGC 2 cut(s) 3013, 3540
BstAUI TGTACA 1 cut(s) 3519
BstDSI CCRYGG 5 cut(s) 157, 204, 280, 423, 666
BstEII GGTNACC 2 cut(s) 2409, 2567
BstENI CCTNNNNNAGG 1 cut(s) 3511
BstF5I GGATG 8 cut(s) 886, 1255, 1458, 1573, 2462, 2925, 3184, 3880
BstFNI CGCG 9 cut(s) 107, 120, 129, 138, 159, 173, 206, 269, 1274
BstH2I RGCGCY 1 cut(s) 18
BstHHI GCGC 6 cut(s) 17, 109, 120, 129, 175, 1276
BstMAI GTCTC 9 cut(s) 286, 378, 739, 815, 1128, 2215, 2738, 3106, 3351
BstMCI CGRYCG 3 cut(s) 195, 210, 289
BstNI CCWGG 9 cut(s) 247, 317, 795, 1040, 1515, 2189, 3431, 3486, 3612
BstNSI RCATGY 5 cut(s) 1317, 1522, 3119, 3597, 3923
BstPI GGTNACC 2 cut(s) 2409, 2567
BstSFI CTRYAG 2 cut(s) 2038, 2883
BstSLI GKGCMC 1 cut(s) 2863
BstUI CGCG 9 cut(s) 107, 120, 129, 138, 159, 173, 206, 269, 1274
BstV1I GCAGC 6 cut(s) 127, 477, 554, 2024, 2897, 3518
BstV2I GAAGAC 7 cut(s) 75, 173, 582, 1443, 1486, 2753, 3568
BstX2I RGATCY 6 cut(s) 273, 1102, 1510, 1645, 2448, 3777
BstYI RGATCY 6 cut(s) 273, 1102, 1510, 1645, 2448, 3777
BstZ17I GTATAC 1 cut(s) 1589
BstZI CGGCCG 2 cut(s) 207, 286
Bsu36I CCTNAGG 3 cut(s) 2415, 2855, 3584
BsuI GTATCC 2 cut(s) 3879, 3909
BsuRI GGCC 9 cut(s) 209, 288, 352, 422, 428, 1234, 1947, 2217, 4064
BtgI CCRYGG 5 cut(s) 157, 204, 280, 423, 666
BtrI CACGTC 1 cut(s) 46
BtsCI GGATG 8 cut(s) 886, 1255, 1458, 1573, 2462, 2925, 3184, 3880
BtsI GCAGTG 3 cut(s) 1407, 1990, 3039
BtuMI TCGCGA 2 cut(s) 138, 269
CciNI GCGGCCGC 1 cut(s) 207
CfoI GCGC 6 cut(s) 17, 109, 120, 129, 175, 1276
Cfr10I RCCGGY 5 cut(s) 304, 824, 1652, 2388, 3864
Cfr13I GGNCC 4 cut(s) 322, 350, 1609, 1637
Cfr42I CCGCGG 2 cut(s) 160, 207
CseI GACGC 4 cut(s) 113, 118, 155, 1280
CsiI ACCWGGT 2 cut(s) 315, 2187
Csp6I GTAC 4 cut(s) 235, 2604, 3520, 3830
CspAI ACCGGT 2 cut(s) 824, 2388
CspCI CAANNNNNGTGG 2 cut(s) 418, 453
CviQI GTAC 4 cut(s) 235, 2604, 3520, 3830
DinI GGCGCC 1 cut(s) 16
DrdI GACNNNNNNGTC 2 cut(s) 17, 690
DriI GACNNNNNGTC 2 cut(s) 370, 4021
DseDI GACNNNNNNGTC 2 cut(s) 17, 690
EaeI YGGCCR 5 cut(s) 207, 286, 426, 1232, 4062
EagI CGGCCG 2 cut(s) 207, 286
Eam1104I CTCTTC 5 cut(s) 768, 1069, 1917, 3117, 4014
Eam1105I GACNNNNNGTC 2 cut(s) 370, 4021
EarI CTCTTC 5 cut(s) 768, 1069, 1917, 3117, 4014
Ecl136II GAGCTC 1 cut(s) 265
EclXI CGGCCG 2 cut(s) 207, 286
Eco130I CCWWGG 6 cut(s) 399, 423, 1640, 2082, 2563, 3507
Eco24I GRGCYC 1 cut(s) 267
Eco31I GGTCTC 2 cut(s) 378, 815
Eco32I GATATC 1 cut(s) 2893
Eco47I GGWCC 3 cut(s) 322, 1609, 1637
Eco52I CGGCCG 2 cut(s) 207, 286
Eco53kI GAGCTC 1 cut(s) 265
Eco57I CTGAAG 7 cut(s) 43, 352, 897, 1229, 1805, 1916, 3983
Eco81I CCTNAGG 3 cut(s) 2415, 2855, 3584
Eco88I CYCGRG 1 cut(s) 2510
Eco91I GGTNACC 2 cut(s) 2409, 2567
EcoICRI GAGCTC 1 cut(s) 265
EcoNI CCTNNNNNAGG 1 cut(s) 3511
EcoO109I RGGNCCY 1 cut(s) 1609
EcoO65I GGTNACC 2 cut(s) 2409, 2567
EcoRI GAATTC 1 cut(s) 3502
EcoRII CCWGG 9 cut(s) 245, 315, 793, 1038, 1513, 2187, 3429, 3484, 3610
EcoRV GATATC 1 cut(s) 2893
EcoT14I CCWWGG 6 cut(s) 399, 423, 1640, 2082, 2563, 3507
EcoT22I ATGCAT 1 cut(s) 2464
EcoT38I GRGCYC 1 cut(s) 267
EgeI GGCGCC 1 cut(s) 16
EheI GGCGCC 1 cut(s) 16
ErhI CCWWGG 6 cut(s) 399, 423, 1640, 2082, 2563, 3507
FalI AAGNNNNNCTT 6 cut(s) 1283, 1315, 1725, 1757, 2366, 2398
FaqI GGGAC 7 cut(s) 56, 1021, 1484, 1591, 3145, 3877, 4028
FauI CCCGC 2 cut(s) 83, 341
FauNDI CATATG 1 cut(s) 3943
FblI GTMKAC 4 cut(s) 1528, 1588, 2160, 2338
FokI GGATG 8 cut(s) 893, 1262, 1465, 1580, 2449, 2932, 3191, 3867
FriOI GRGCYC 1 cut(s) 267
FspBI CTAG 6 cut(s) 848, 1277, 1604, 1613, 3069, 4244
GlaI GCGC 6 cut(s) 16, 108, 119, 128, 174, 1275
GsaI CCCAGC 1 cut(s) 450
GsuI CTGGAG 2 cut(s) 229, 3452
HaeII RGCGCY 1 cut(s) 18
HaeIII GGCC 9 cut(s) 209, 288, 352, 422, 428, 1234, 1947, 2217, 4064
HgaI GACGC 4 cut(s) 113, 118, 155, 1280
HhaI GCGC 6 cut(s) 17, 109, 120, 129, 175, 1276
Hin1I GRCGYC 3 cut(s) 15, 147, 333
Hin6I GCGC 6 cut(s) 15, 107, 118, 127, 173, 1274
HinP1I GCGC 6 cut(s) 15, 107, 118, 127, 173, 1274
HincII GTYRAC 1 cut(s) 898
HindII GTYRAC 1 cut(s) 898
HindIII AAGCTT 3 cut(s) 2090, 2798, 3052
HpaI GTTAAC 1 cut(s) 898
HphI GGTGA 6 cut(s) 79, 1397, 3215, 4007, 4096, 4163
HpyCH4IV ACGT 3 cut(s) 45, 333, 612
HpySE526I ACGT 3 cut(s) 45, 333, 612
Hsp92I GRCGYC 3 cut(s) 15, 147, 333
HspAI GCGC 6 cut(s) 15, 107, 118, 127, 173, 1274
KasI GGCGCC 1 cut(s) 14
KspAI GTTAAC 1 cut(s) 898
KspI CCGCGG 2 cut(s) 160, 207
Lsp1109I GCAGC 6 cut(s) 127, 477, 554, 2024, 2897, 3518
LweI GCATC 7 cut(s) 792, 1195, 1339, 1443, 2471, 2910, 3169
MabI ACCWGGT 2 cut(s) 315, 2187
MaeI CTAG 6 cut(s) 848, 1277, 1604, 1613, 3069, 4244
MaeII ACGT 3 cut(s) 45, 333, 612
MbiI CCGCTC 1 cut(s) 348
MfeI CAATTG 6 cut(s) 1353, 1395, 2258, 2610, 2844, 3873
MflI RGATCY 6 cut(s) 273, 1102, 1510, 1645, 2448, 3777
MhlI GDGCHC 4 cut(s) 225, 267, 2863, 3309
MlsI TGGCCA 2 cut(s) 428, 4064
MluNI TGGCCA 2 cut(s) 428, 4064
Mly113I GGCGCC 1 cut(s) 15
MlyI GAGTC 6 cut(s) 187, 194, 236, 1043, 1774, 4031
MmeI TCCRAC 3 cut(s) 354, 1748, 2092
Mox20I TGGCCA 2 cut(s) 428, 4064
Mph1103I ATGCAT 1 cut(s) 2464
MroXI GAANNNNTTC 2 cut(s) 365, 3064
MscI TGGCCA 2 cut(s) 428, 4064
MslI CAYNNNNRTG 2 cut(s) 2918, 3302
Msp20I TGGCCA 2 cut(s) 428, 4064
MspA1I CMGCKG 3 cut(s) 159, 206, 3551
MspCI CTTAAG 1 cut(s) 2099
MunI CAATTG 6 cut(s) 1353, 1395, 2258, 2610, 2844, 3873
Mva1269I GAATGC 3 cut(s) 3066, 3500, 4170
MvaI CCWGG 9 cut(s) 247, 317, 795, 1040, 1515, 2189, 3431, 3486, 3612
MvnI CGCG 9 cut(s) 107, 120, 129, 138, 159, 173, 206, 269, 1274
NarI GGCGCC 1 cut(s) 15
NciI CCSGG 1 cut(s) 530
NcoI CCATGG 1 cut(s) 423
NdeI CATATG 1 cut(s) 3943
NlaIV GGNNCC 7 cut(s) 16, 35, 254, 324, 1512, 1638, 2398
NmeAIII GCCGAG 2 cut(s) 201, 255
NmuCI GTSAC 4 cut(s) 608, 808, 3034, 4023
NotI GCGGCCGC 1 cut(s) 207
NruI TCGCGA 2 cut(s) 138, 269
NsiI ATGCAT 1 cut(s) 2464
NspI RCATGY 5 cut(s) 1317, 1522, 3119, 3597, 3923
PaeI GCATGC 2 cut(s) 1317, 1522
PaeR7I CTCGAG 1 cut(s) 2510
PciI ACATGT 2 cut(s) 3115, 3919
PcsI WCGNNNNNNNCGW 3 cut(s) 1278, 1494, 4014
PctI GAATGC 3 cut(s) 3066, 3500, 4170
PdmI GAANNNNTTC 2 cut(s) 365, 3064
PflFI GACNNNGTC 1 cut(s) 2673
PinAI ACCGGT 2 cut(s) 824, 2388
Ple19I CGATCG 1 cut(s) 195
PleI GAGTC 6 cut(s) 186, 194, 236, 1042, 1773, 4030
PluTI GGCGCC 1 cut(s) 18
PpsI GAGTC 6 cut(s) 186, 194, 236, 1042, 1773, 4030
PpuMI RGGWCCY 1 cut(s) 1609
PscI ACATGT 2 cut(s) 3115, 3919
PshBI ATTAAT 1 cut(s) 1554
PsiI TTATAA 1 cut(s) 1005
Psp124BI GAGCTC 1 cut(s) 267
Psp5II RGGWCCY 1 cut(s) 1609
Psp6I CCWGG 9 cut(s) 245, 315, 793, 1038, 1513, 2187, 3429, 3484, 3610
PspEI GGTNACC 2 cut(s) 2409, 2567
PspFI CCCAGC 1 cut(s) 446
PspGI CCWGG 9 cut(s) 245, 315, 793, 1038, 1513, 2187, 3429, 3484, 3610
PspN4I GGNNCC 7 cut(s) 16, 35, 254, 324, 1512, 1638, 2398
PspPI GGNCC 4 cut(s) 322, 350, 1609, 1637
PspPPI RGGWCCY 1 cut(s) 1609
PsrI GAACNNNNNNTAC 2 cut(s) 2419, 2451
PstI CTGCAG 2 cut(s) 2042, 2887
PsuI RGATCY 6 cut(s) 273, 1102, 1510, 1645, 2448, 3777
PsyI GACNNNGTC 1 cut(s) 2673
PvuI CGATCG 1 cut(s) 195
PvuII CAGCTG 1 cut(s) 3551
RruI TCGCGA 2 cut(s) 138, 269
RsaI GTAC 4 cut(s) 236, 2605, 3521, 3831
RsaNI GTAC 4 cut(s) 235, 2604, 3520, 3830
RseI CAYNNNNRTG 2 cut(s) 2918, 3302
SacI GAGCTC 1 cut(s) 267
SacII CCGCGG 2 cut(s) 160, 207
Sau96I GGNCC 4 cut(s) 322, 350, 1609, 1637
SchI GAGTC 6 cut(s) 187, 194, 236, 1043, 1774, 4031
SduI GDGCHC 4 cut(s) 225, 267, 2863, 3309
SexAI ACCWGGT 2 cut(s) 315, 2187
SfaNI GCATC 7 cut(s) 792, 1195, 1339, 1443, 2471, 2910, 3169
SfcI CTRYAG 2 cut(s) 2038, 2883
SfoI GGCGCC 1 cut(s) 16
Sfr274I CTCGAG 1 cut(s) 2510
Sfr303I CCGCGG 2 cut(s) 160, 207
SgrBI CCGCGG 2 cut(s) 160, 207
SinI GGWCC 3 cut(s) 322, 1609, 1637
SlaI CTCGAG 1 cut(s) 2510
SmiMI CAYNNNNRTG 2 cut(s) 2918, 3302
SmlI CTYRAG 9 cut(s) 257, 2099, 2228, 2510, 2792, 2801, 3341, 3364, 3639
SmoI CTYRAG 9 cut(s) 257, 2099, 2228, 2510, 2792, 2801, 3341, 3364, 3639
SpeI ACTAGT 1 cut(s) 847
SphI GCATGC 2 cut(s) 1317, 1522
SspDI GGCGCC 1 cut(s) 14
SspMI CTAG 6 cut(s) 848, 1277, 1604, 1613, 3069, 4244
SstI GAGCTC 1 cut(s) 267
StyI CCWWGG 6 cut(s) 399, 423, 1640, 2082, 2563, 3507
TaiI ACGT 3 cut(s) 48, 336, 615
TaqI TCGA 4 cut(s) 195, 2135, 2511, 3222
TatI WGTACW 1 cut(s) 3519
TauI GCSGC 7 cut(s) 123, 162, 209, 212, 288, 3276, 3940
TseFI GTSAC 4 cut(s) 608, 808, 3034, 4023
TseI GCWGC 6 cut(s) 115, 465, 542, 2037, 2885, 3531
Tsp45I GTSAC 4 cut(s) 608, 808, 3034, 4023
TspGWI ACGGA 1 cut(s) 3292
Tth111I GACNNNGTC 1 cut(s) 2673
Vha464I CTTAAG 1 cut(s) 2099
VpaK11BI GGWCC 3 cut(s) 322, 1609, 1637
VspI ATTAAT 1 cut(s) 1554
XagI CCTNNNNNAGG 1 cut(s) 3511
XceI RCATGY 5 cut(s) 1317, 1522, 3119, 3597, 3923
XcmI CCANNNNNNNNNTGG 4 cut(s) 2135, 2497, 2920, 3902
XhoI CTCGAG 1 cut(s) 2510
XmiI GTMKAC 4 cut(s) 1528, 1588, 2160, 2338
XmnI GAANNNNTTC 2 cut(s) 365, 3064
XspI CTAG 6 cut(s) 848, 1277, 1604, 1613, 3069, 4244
ZraI GACGTC 1 cut(s) 334
Zsp2I ATGCAT 1 cut(s) 2464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.