RLG00000005773

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0001217
Physical Location & Seq
Forward (+)
1621354 .. 1623777
2424 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005773

Sequence Viewer

Length: 1902 bp
ATGGGTCTCATTCCGTACCCGGTGGGACTTCATTCCCGAGTTGAAGAGGTGATGAAGCTGTTAGATATCGGATCCAAAACCGTTCAAGTTGTGGGAGTTCATGGTATGGGCGGGATTGGCAAAACAACCCTTGCCAAGGCTCTTTTCAATAGACTTGTTGGTCATTTTGAGCGTCACAGACACAGCTTCATCTCAAATATTAGAGAAATTTCAGCAGGACGTGATGAGAAGGGCTTAGTTACTCTTCAAAACAAACTTATCAGTGATCTTTCCAATGGGACGGTGGCTAAAATTAATCAGACTAGTGCTGGTATCGCTGCCATCAAAGCGGCTGTGAATGAGAAGCGAGTCCTTGTTGTTCTGGATGATGTTGATAACATAGATGTACTAAGTGCTTTAGTTGGCAACGGACAATGCAGAGAACTCTTATCTAATCATCGTGTAAATCATAAGTTGTATGAGGTCAGGGAGTTGGATGGATCCGATGCTCTAGAACTTTTCAGTCACCATGCACTGGGAATTAGAGAAATACCGGCAGATACCTTTCTGAAACTATCTAAGGAGATTGTGGATCTTACTGGTGGGCTACCATTGGCGCTTGAAGTATTTGGCTCTAGCCTCTATGATAAGAGGACAGAAAAGGTATGGATAGATGCTCTGCACAAACAGAGACAGATTCGTCCACGGGATCTTCAGGATGTCCTGATGATAAGCTACAATGCTCTAGATGACCAGGAGAAGTGCATCTTTCTAGACATTGCATGTTTATTTGTTAAAATGAAGACCAAAAGAGAAGACGCAATTGTCATATTGAAGGGTTGTGGTTTTGATGGTGAGATAGTACTTACTGTCCTCACAGCAAGATCACTCATTAAGATCACTGAAGACACTACATTATGGATGCATGATCAAGTCAGAGACATGGGAAGACAAATCAGTAGTAGACTGTGGGATCGCGATGAAATCATGAATGTTTTGAAGTATGATAAGGGAACACCATCTATACAAGGGATTGTCCTAGACTTTGAAATGAAAAGGATGGTGAGTGATATAGGAGGTGACAGAATTTCTTGGGACAACTTTCGGAGAAGCCCCAATTGTACCACTGCATGGACATACCTAAAGGAAAGATACAAAGCACATCGTAAAGGCCAAGCAGAGAAAAAGGGAGAGGTTAACATTTCCTCTAAAGCTTTTGGAGCAATGGTCAATCTTAGGCTGCTTCAAATGAATAATGTGAATTTAGAAGGGAACTCAAAGTTTCTTCCTGCTGGTGTCAAGTGGTTACAATGGAAAGGTTGTCCTTTGAGATCTCTCCCTTCTGATTTCTTGCCTCCGCAACTCGCTGTCCTTGATCTTTCAGACAGCAATATCACAAGTTTGTGCGGAGGGTGCAGTATCATGGTATGCTTCAATTGTCTGATCTGCAGTGGAGACGGAAACAAGGTGGCTGAGAAATTGATGTTCTTGAATCTAAGCGACTGCATTTACCTAACTGCTATTCCTGATTTATCTGGGAATCGAACGCTACAACAGCTTAATCTTGAGCACTGTATTTCACTGATTAAGCTTCATGGCTCGATTGGGAACCTGAATACATTAGTGCACTTGAACCTTCGAGAGTGTTCAAAGCTTGTTGAACTTCCAAGTGATGTCTCCGGCCTGAAAAAGCTTGAGTACCTAATCCTACCTGGTTGCTCACAGTTCAAAAAACTGCCAAACATGGATAGCTTGGTGTCACTGAAAGAACTTCTCCTTGATGACACTGCTATACAAAGTCTTCCCCAATCTATCTTCCGGCTTACAAAACTTGAAAAGCTCAGCTTAAACCGGTGCAGTGAATTAAAAGAGCTACCTGTGGAGATTAGTCAGAAAGAACGAGAAGATTGGGAGATTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

634

Amino Acids

70.89

Weight (kDa)

7.82

Isoelectric Point (pI)

35.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_16 PF13191 12 - 131 5.1e-07 AAA ATPase domain
NB-ARC PF00931 13 - 127 9.4e-13 NB-ARC domain
ATPase_2 PF01637 13 - 128 3.2e-06 ATPase domain predominantly from Archaea
WHD_ROQ1 PF23282 244 - 312 2.1e-13 Disease resistance protein Roq1-like, winged-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 159, 678, 803
AccB7I CCANNNNNTGG 2 cut(s) 514, 1110
AccI GTMKAC 1 cut(s) 943
AccII CGCG 1 cut(s) 957
AciI CCGC 4 cut(s) 111, 329, 1337, 1386
AclWI GGATC 7 cut(s) 66, 79, 474, 487, 579, 696, 960
AcsI RAATTY 3 cut(s) 207, 1065, 1240
AcuI CTGAAG 2 cut(s) 677, 903
AfaI GTAC 5 cut(s) 17, 387, 843, 1102, 1679
AfiI CCNNNNNNNGG 3 cut(s) 136, 514, 1110
AgeI ACCGGT 1 cut(s) 1830
AhlI ACTAGT 1 cut(s) 302
AjiI CACGTC 1 cut(s) 221
AjnI CCWGG 2 cut(s) 732, 1690
AjuI GAANNNNNNNTTGG 2 cut(s) 128, 160
Alw21I GWGCWC 2 cut(s) 1551, 1608
Alw26I GTCTC 5 cut(s) 11, 664, 912, 1428, 1660
Alw44I GTGCAC 1 cut(s) 1604
AlwI GGATC 7 cut(s) 66, 79, 474, 487, 579, 696, 960
Ama87I CYCGRG 1 cut(s) 36
AoxI GGCC 2 cut(s) 1150, 1660
ApaLI GTGCAC 1 cut(s) 1604
ApeKI GCWGC 2 cut(s) 317, 1219
ApoI RAATTY 3 cut(s) 207, 1065, 1240
AseI ATTAAT 1 cut(s) 294
AsiGI ACCGGT 1 cut(s) 1830
AspLEI GCGC 1 cut(s) 598
AsuC2I CCSGG 1 cut(s) 20
AsuHPI GGTGA 5 cut(s) 61, 497, 845, 1054, 1070
AvaI CYCGRG 1 cut(s) 36
BaeGI GKGCMC 1 cut(s) 1608
BamHI GGATCC 2 cut(s) 71, 479
BbsI GAAGAC 5 cut(s) 788, 801, 891, 934, 1772
Bbv12I GWGCWC 2 cut(s) 1551, 1608
BbvI GCAGC 2 cut(s) 304, 1206
BccI CCATC 5 cut(s) 329, 470, 824, 1006, 1033
BciT130I CCWGG 2 cut(s) 734, 1692
BclI TGATCA 1 cut(s) 907
BcnI CCSGG 1 cut(s) 20
BcoDI GTCTC 5 cut(s) 11, 664, 912, 1428, 1660
BcuI ACTAGT 1 cut(s) 302
BfaI CTAG 6 cut(s) 303, 491, 615, 725, 752, 1019
BfmI CTRYAG 1 cut(s) 1426
BfoI RGCGCY 1 cut(s) 599
BglII AGATCT 1 cut(s) 1310
BisI GCNGC 3 cut(s) 318, 330, 1220
BlpI GCTNAGC 1 cut(s) 1820
BlsI GCNGC 3 cut(s) 319, 331, 1221
BmcAI AGTACT 1 cut(s) 843
Bme1390I CCNGG 3 cut(s) 20, 734, 1692
BmeT110I CYCGRG 1 cut(s) 36
BmgBI CACGTC 1 cut(s) 221
BmiI GGNNCC 3 cut(s) 73, 481, 1589
BmrFI CCNGG 3 cut(s) 20, 734, 1692
BmrI ACTGGG 1 cut(s) 524
BmsI GCATC 4 cut(s) 475, 643, 753, 891
BmuI ACTGGG 1 cut(s) 524
BpiI GAAGAC 5 cut(s) 788, 801, 891, 934, 1772
Bpu1102I GCTNAGC 1 cut(s) 1820
BpuEI CTTGAG 2 cut(s) 1565, 1694
BpuMI CCSGG 1 cut(s) 20
BsaI GGTCTC 1 cut(s) 11
BsaJI CCNNGG 2 cut(s) 135, 683
BsaWI WCCGGW 1 cut(s) 1830
BsaXI ACNNNNNCTCC 2 cut(s) 1191, 1221
Bsc4I CCNNNNNNNGG 3 cut(s) 136, 514, 1110
Bse118I RCCGGY 2 cut(s) 532, 1830
Bse1I ACTGG 2 cut(s) 519, 583
Bse3DI GCAATG 2 cut(s) 756, 1209
BseBI CCWGG 2 cut(s) 734, 1692
BseDI CCNNGG 2 cut(s) 135, 683
BseGI GGATG 5 cut(s) 370, 481, 703, 906, 1044
BseLI CCNNNNNNNGG 3 cut(s) 136, 514, 1110
BseMI GCAATG 2 cut(s) 756, 1209
BseMII CTCAG 2 cut(s) 1443, 1834
BseNI ACTGG 2 cut(s) 519, 583
BseSI GKGCMC 1 cut(s) 1608
BseXI GCAGC 2 cut(s) 304, 1206
BsgI GTGCAG 3 cut(s) 644, 1414, 1855
Bsh1236I CGCG 1 cut(s) 957
BshFI GGCC 2 cut(s) 1152, 1662
BshTI ACCGGT 1 cut(s) 1830
BsiHKAI GWGCWC 2 cut(s) 1551, 1608
BsiHKCI CYCGRG 1 cut(s) 36
BsiSI CCGG 5 cut(s) 20, 533, 1659, 1798, 1831
BslFI GGGAC 3 cut(s) 39, 292, 1088
BslI CCNNNNNNNGG 3 cut(s) 136, 514, 1110
BsmAI GTCTC 5 cut(s) 11, 664, 912, 1428, 1660
BsmBI CGTCTC 1 cut(s) 1428
BsmFI GGGAC 3 cut(s) 39, 292, 1088
BsnI GGCC 2 cut(s) 1152, 1662
Bso31I GGTCTC 1 cut(s) 11
BsoBI CYCGRG 1 cut(s) 36
Bsp1286I GDGCHC 2 cut(s) 1551, 1608
Bsp1720I GCTNAGC 1 cut(s) 1820
Bsp68I TCGCGA 1 cut(s) 957
BspACI CCGC 4 cut(s) 111, 329, 1337, 1386
BspANI GGCC 2 cut(s) 1152, 1662
BspCNI CTCAG 2 cut(s) 1444, 1833
BspFNI CGCG 1 cut(s) 957
BspHI TCATGA 1 cut(s) 966
BspLI GGNNCC 3 cut(s) 73, 481, 1589
BspMAI CTGCAG 1 cut(s) 1430
BspPI GGATC 7 cut(s) 66, 79, 474, 487, 579, 696, 960
BspTNI GGTCTC 1 cut(s) 11
BsrDI GCAATG 2 cut(s) 756, 1209
BsrFI RCCGGY 2 cut(s) 532, 1830
BsrI ACTGG 2 cut(s) 519, 583
BssAI RCCGGY 2 cut(s) 532, 1830
BssECI CCNNGG 2 cut(s) 135, 683
BssT1I CCWWGG 1 cut(s) 135
Bst2UI CCWGG 2 cut(s) 734, 1692
Bst4CI ACNGT 6 cut(s) 82, 283, 850, 948, 1553, 1704
Bst6I CTCTTC 2 cut(s) 39, 249
BstDEI CTNAG 7 cut(s) 235, 389, 558, 1214, 1452, 1475, 1820
BstDSI CCRYGG 1 cut(s) 683
BstENI CCTNNNNNAGG 1 cut(s) 134
BstF5I GGATG 5 cut(s) 370, 481, 703, 906, 1044
BstFNI CGCG 1 cut(s) 957
BstH2I RGCGCY 1 cut(s) 599
BstHHI GCGC 1 cut(s) 598
BstMAI GTCTC 5 cut(s) 11, 664, 912, 1428, 1660
BstMWI GCNNNNNNNGC 6 cut(s) 117, 314, 326, 1199, 1392, 1534
BstNI CCWGG 2 cut(s) 734, 1692
BstNSI RCATGY 1 cut(s) 765
BstSCI CCNGG 3 cut(s) 18, 732, 1690
BstSFI CTRYAG 1 cut(s) 1426
BstSLI GKGCMC 1 cut(s) 1608
BstUI CGCG 1 cut(s) 957
BstV1I GCAGC 2 cut(s) 304, 1206
BstV2I GAAGAC 5 cut(s) 788, 801, 891, 934, 1772
BstX2I RGATCY 5 cut(s) 71, 479, 571, 688, 1310
BstYI RGATCY 5 cut(s) 71, 479, 571, 688, 1310
BsuRI GGCC 2 cut(s) 1152, 1662
BtgI CCRYGG 1 cut(s) 683
BtgZI GCGATG 1 cut(s) 972
BtrI CACGTC 1 cut(s) 221
BtsCI GGATG 5 cut(s) 370, 481, 703, 906, 1044
BtsI GCAGTG 4 cut(s) 1104, 1435, 1764, 1843
BtuMI TCGCGA 1 cut(s) 957
CciI TCATGA 1 cut(s) 966
CfoI GCGC 1 cut(s) 598
Cfr10I RCCGGY 2 cut(s) 532, 1830
CseI GACGC 2 cut(s) 161, 806
CsiI ACCWGGT 1 cut(s) 1690
Csp6I GTAC 5 cut(s) 16, 386, 842, 1101, 1678
CspAI ACCGGT 1 cut(s) 1830
CviQI GTAC 5 cut(s) 16, 386, 842, 1101, 1678
DdeI CTNAG 7 cut(s) 235, 389, 558, 1214, 1452, 1475, 1820
DrdI GACNNNNNNGTC 3 cut(s) 159, 678, 803
DseDI GACNNNNNNGTC 3 cut(s) 159, 678, 803
Eam1104I CTCTTC 2 cut(s) 39, 249
EarI CTCTTC 2 cut(s) 39, 249
Eco130I CCWWGG 1 cut(s) 135
Eco31I GGTCTC 1 cut(s) 11
Eco32I GATATC 1 cut(s) 67
Eco57I CTGAAG 2 cut(s) 677, 903
Eco88I CYCGRG 1 cut(s) 36
EcoNI CCTNNNNNAGG 1 cut(s) 134
EcoRII CCWGG 2 cut(s) 732, 1690
EcoRV GATATC 1 cut(s) 67
EcoT14I CCWWGG 1 cut(s) 135
EcoT22I ATGCAT 1 cut(s) 906
ErhI CCWWGG 1 cut(s) 135
Esp3I CGTCTC 1 cut(s) 1428
FalI AAGNNNNNCTT 4 cut(s) 731, 763, 1808, 1840
FaqI GGGAC 3 cut(s) 39, 292, 1088
FauI CCCGC 1 cut(s) 104
FbaI TGATCA 1 cut(s) 907
FblI GTMKAC 1 cut(s) 943
Fnu4HI GCNGC 3 cut(s) 318, 330, 1220
FokI GGATG 5 cut(s) 377, 488, 710, 913, 1051
Fsp4HI GCNGC 3 cut(s) 318, 330, 1220
FspBI CTAG 6 cut(s) 303, 491, 615, 725, 752, 1019
GlaI GCGC 1 cut(s) 597
GluI GCNGC 3 cut(s) 318, 330, 1220
HaeII RGCGCY 1 cut(s) 599
HaeIII GGCC 2 cut(s) 1152, 1662
HapII CCGG 5 cut(s) 20, 533, 1659, 1798, 1831
HgaI GACGC 2 cut(s) 161, 806
HhaI GCGC 1 cut(s) 598
Hin6I GCGC 1 cut(s) 596
HinP1I GCGC 1 cut(s) 596
HincII GTYRAC 1 cut(s) 1177
HindII GTYRAC 1 cut(s) 1177
HindIII AAGCTT 4 cut(s) 1191, 1568, 1631, 1670
HinfI GANTC 4 cut(s) 348, 676, 1471, 1519
HpaI GTTAAC 1 cut(s) 1177
HpaII CCGG 5 cut(s) 20, 533, 1659, 1798, 1831
HphI GGTGA 5 cut(s) 61, 497, 845, 1054, 1070
Hpy166II GTNNAC 4 cut(s) 683, 944, 1177, 1606
Hpy8I GTNNAC 4 cut(s) 683, 944, 1177, 1606
HpyAV CCTTC 5 cut(s) 223, 808, 1241, 1329, 1625
HpyCH4III ACNGT 6 cut(s) 82, 283, 850, 948, 1553, 1704
HpyCH4IV ACGT 1 cut(s) 220
HpyF10VI GCNNNNNNNGC 6 cut(s) 117, 314, 326, 1199, 1392, 1534
HpyF3I CTNAG 7 cut(s) 235, 389, 558, 1214, 1452, 1475, 1820
HpySE526I ACGT 1 cut(s) 220
HspAI GCGC 1 cut(s) 596
Ksp22I TGATCA 1 cut(s) 907
KspAI GTTAAC 1 cut(s) 1177
LmnI GCTCC 1 cut(s) 1199
Lsp1109I GCAGC 2 cut(s) 304, 1206
LweI GCATC 4 cut(s) 475, 643, 753, 891
MabI ACCWGGT 1 cut(s) 1690
MaeI CTAG 6 cut(s) 303, 491, 615, 725, 752, 1019
MaeII ACGT 1 cut(s) 220
MaeIII GTNAC 6 cut(s) 173, 238, 503, 1058, 1284, 1737
MfeI CAATTG 3 cut(s) 801, 1096, 1414
MflI RGATCY 5 cut(s) 71, 479, 571, 688, 1310
MhlI GDGCHC 2 cut(s) 1551, 1608
MlyI GAGTC 1 cut(s) 357
MmeI TCCRAC 1 cut(s) 453
Mph1103I ATGCAT 1 cut(s) 906
MseI TTAA 8 cut(s) 294, 774, 873, 1176, 1539, 1566, 1826, 1844
MspI CCGG 5 cut(s) 20, 533, 1659, 1798, 1831
MspR9I CCNGG 3 cut(s) 20, 734, 1692
MunI CAATTG 3 cut(s) 801, 1096, 1414
MvaI CCWGG 2 cut(s) 734, 1692
MvnI CGCG 1 cut(s) 957
MwoI GCNNNNNNNGC 6 cut(s) 117, 314, 326, 1199, 1392, 1534
NciI CCSGG 1 cut(s) 20
NlaIV GGNNCC 3 cut(s) 73, 481, 1589
NmuCI GTSAC 4 cut(s) 173, 503, 1058, 1737
NruI TCGCGA 1 cut(s) 957
NsiI ATGCAT 1 cut(s) 906
NspI RCATGY 1 cut(s) 765
PagI TCATGA 1 cut(s) 966
PfeI GAWTC 3 cut(s) 676, 1471, 1519
PflMI CCANNNNNTGG 2 cut(s) 514, 1110
PinAI ACCGGT 1 cut(s) 1830
PkrI GCNGC 3 cut(s) 319, 331, 1221
PleI GAGTC 1 cut(s) 356
PpsI GAGTC 1 cut(s) 356
PshBI ATTAAT 1 cut(s) 294
Psp6I CCWGG 2 cut(s) 732, 1690
PspGI CCWGG 2 cut(s) 732, 1690
PspN4I GGNNCC 3 cut(s) 73, 481, 1589
PstI CTGCAG 1 cut(s) 1430
PsuI RGATCY 5 cut(s) 71, 479, 571, 688, 1310
RruI TCGCGA 1 cut(s) 957
RsaI GTAC 5 cut(s) 17, 387, 843, 1102, 1679
RsaNI GTAC 5 cut(s) 16, 386, 842, 1101, 1678
SaqAI TTAA 8 cut(s) 294, 774, 873, 1176, 1539, 1566, 1826, 1844
SatI GCNGC 3 cut(s) 318, 330, 1220
ScaI AGTACT 1 cut(s) 843
SchI GAGTC 1 cut(s) 357
ScrFI CCNGG 3 cut(s) 20, 734, 1692
SduI GDGCHC 2 cut(s) 1551, 1608
SexAI ACCWGGT 1 cut(s) 1690
SfaNI GCATC 4 cut(s) 475, 643, 753, 891
SfcI CTRYAG 1 cut(s) 1426
SmlI CTYRAG 2 cut(s) 1544, 1673
SmoI CTYRAG 2 cut(s) 1544, 1673
SpeI ACTAGT 1 cut(s) 302
SsiI CCGC 4 cut(s) 111, 329, 1337, 1386
SspI AATATT 1 cut(s) 199
SspMI CTAG 6 cut(s) 303, 491, 615, 725, 752, 1019
StyD4I CCNGG 3 cut(s) 18, 732, 1690
StyI CCWWGG 1 cut(s) 135
TaaI ACNGT 6 cut(s) 82, 283, 850, 948, 1553, 1704
TaiI ACGT 1 cut(s) 223
TaqI TCGA 3 cut(s) 1522, 1580, 1618
TatI WGTACW 2 cut(s) 385, 841
TauI GCSGC 1 cut(s) 332
TfiI GAWTC 3 cut(s) 676, 1471, 1519
Tru1I TTAA 8 cut(s) 294, 774, 873, 1176, 1539, 1566, 1826, 1844
Tru9I TTAA 8 cut(s) 294, 774, 873, 1176, 1539, 1566, 1826, 1844
TseFI GTSAC 4 cut(s) 173, 503, 1058, 1737
TseI GCWGC 2 cut(s) 317, 1219
Tsp45I GTSAC 4 cut(s) 173, 503, 1058, 1737
TspGWI ACGGA 2 cut(s) 423, 1452
Van91I CCANNNNNTGG 2 cut(s) 514, 1110
VneI GTGCAC 1 cut(s) 1604
VspI ATTAAT 1 cut(s) 294
XagI CCTNNNNNAGG 1 cut(s) 134
XapI RAATTY 3 cut(s) 207, 1065, 1240
XbaI TCTAGA 3 cut(s) 490, 724, 751
XceI RCATGY 1 cut(s) 765
XcmI CCANNNNNNNNNTGG 1 cut(s) 280
XmiI GTMKAC 1 cut(s) 943
XspI CTAG 6 cut(s) 303, 491, 615, 725, 752, 1019
ZrmI AGTACT 1 cut(s) 843
Zsp2I ATGCAT 1 cut(s) 906
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.