Rorug04G0358200

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001217
Physical Location & Seq
Reverse (-)
53115054 .. 53117188
2135 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0358200.1

Sequence Viewer

Length: 1251 bp
ATGAAGGATCATAATCAAAACCCTTCAAACTTCTTCACAAAACTAATCAATGTCCAAGGCCACCTCCTCAATCTCCTCTCTTATTTCCTCATCTTTGGTGGTGGTTTAGTTCTCGGAATCGTACTTGCCTCTTCTTATCTCAAAGACTCTTCCTTTAGTCTACAAATAGCCCAACTATCATTCTCCTCTTCTTCTTCTTCATCATCTCCGTCCAATAGTTCCAATATTTCACATATGACAATTAGTCCAAATGACACAGTCACGATGTCTAACATGACCGAAGTTCATATAGGAGAATTAGAGGCATTTCTAAAGCCGCCTAAGGTTCGGCACGACATGGACGATGAGGAATTGTTCTGGAGAGCTTCCATGGCCCCTCGGATACCTGAATATCCGTTCCACCGAGTTCCGAAAGTTGCTTTCATGTTCTTGACAAGGGGACCTGTTCATTTGGCTCCATTGTGGGAGAAGTTCTTCCAAGGGCACCAAGGCTTGTTCTCAATCTATGTCCACTCAGATCCGTCTTATAATGAATCGTCTCACCCCGAAAGCCCAGTTTTTCATGGCCGAAGAATTCCAAGTCAGAGAGCAGAATGGGGGCAAGTGAACATGATTGAAGCCGAGAGCCGCGTAATTGCGAATGCTCTTCTCGACATCTCAAACCAACGCTTTGTTCTGCTTTCAGAATCATGCATCCCCCTCTTCAATTTCTCCACAGTCTACTCTTACCTCATAAACACCAACAAGACTTTTGTGCAGGTCTATGATGATCCAAGCGGAGTTGGGCGTGGCCGATATGATGAAAGCTACTACCCTCAAATCACATTGGACCAGTGGAGGAAAGGGTCGCAGTGGGTTGAAATAGATAGAGACCTTGCCATTGAAGTTGTTTCAGATCAAAAATACTACACAACTTTCCAGAAACACTGCAAAGGCTATTGCTTTGCAGATGAGCATTACTTGCCAACATTTATGAACATAAGATTTCCGAAAAAGAATTCAAACAGGACTGTGACCTGGGTTGATTGGTCTAGGGGCGGACCTCATCCTGTCGAGTACGCGAGCACAGATGTCACGGTTGAGCTTCTGAATACTCTGAGGAAGAGTAACGGCTGGTGTGATTACAATGGAAGGGGCACAGATGTTTGTTTCTTGTTCGCCCGGAAGTTCTCGCCAAATTCTTTGGATAACCTGCTAAGGATTGCACCACAGTTAATGCAATTCAACAGAGACAGTGATAAAGTGTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

416

Amino Acids

47.7

Weight (kDa)

6.53

Isoelectric Point (pI)

42.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 139 - 366 3.2e-78 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 528
Acc36I ACCTGC 2 cut(s) 748, 1200
AccB1I GGYRCC 1 cut(s) 483
AccI GTMKAC 2 cut(s) 160, 720
AccII CGCG 2 cut(s) 630, 1061
AciI CCGC 4 cut(s) 317, 628, 777, 1038
AclWI GGATC 3 cut(s) 15, 512, 764
AcoI YGGCCR 2 cut(s) 565, 790
AcsI RAATTY 3 cut(s) 573, 997, 1177
AfaI GTAC 2 cut(s) 123, 1058
AgsI TTSAA 7 cut(s) 27, 617, 706, 860, 884, 1002, 1225
AhdI GACNNNNNGTC 1 cut(s) 243
AjnI CCWGG 1 cut(s) 1016
AjuI GAANNNNNNNTTGG 2 cut(s) 657, 689
AluBI AGCT 3 cut(s) 365, 807, 1084
AluI AGCT 3 cut(s) 365, 807, 1084
Alw21I GWGCWC 1 cut(s) 1067
Alw26I GTCTC 3 cut(s) 543, 864, 1224
AlwI GGATC 3 cut(s) 15, 512, 764
AoxI GGCC 4 cut(s) 58, 372, 565, 790
ApoI RAATTY 3 cut(s) 573, 997, 1177
Asp700I GAANNNNTTC 1 cut(s) 473
AspS9I GGNCC 4 cut(s) 373, 440, 829, 1040
AsuC2I CCSGG 1 cut(s) 1162
AsuHPI GGTGA 1 cut(s) 533
AvaII GGWCC 3 cut(s) 440, 829, 1040
AxyI CCTNAGG 1 cut(s) 321
BaeGI GKGCMC 2 cut(s) 486, 1139
BanI GGYRCC 1 cut(s) 483
Bbv12I GWGCWC 1 cut(s) 1067
BceAI ACGGC 1 cut(s) 1126
BciT130I CCWGG 1 cut(s) 1018
BciVI GTATCC 1 cut(s) 375
BcnI CCSGG 1 cut(s) 1162
BcoDI GTCTC 3 cut(s) 543, 864, 1224
BfaI CTAG 1 cut(s) 1032
BfuAI ACCTGC 2 cut(s) 748, 1200
BfuI GTATCC 1 cut(s) 375
BisI GCNGC 2 cut(s) 317, 628
BlsI GCNGC 2 cut(s) 318, 629
Bme1390I CCNGG 2 cut(s) 1018, 1162
Bme18I GGWCC 3 cut(s) 440, 829, 1040
BmeRI GACNNNNNGTC 1 cut(s) 243
BmgT120I GGNCC 4 cut(s) 373, 440, 829, 1040
BmiI GGNNCC 4 cut(s) 375, 441, 456, 485
BmrFI CCNGG 2 cut(s) 1018, 1162
BmrI ACTGGG 1 cut(s) 548
BmsI GCATC 1 cut(s) 702
BmuI ACTGGG 1 cut(s) 548
BpmI CTGGAG 1 cut(s) 379
Bpu10I CCTNAGC 1 cut(s) 1196
BpuMI CCSGG 1 cut(s) 1162
BsaBI GATNNNNATC 1 cut(s) 12
BsaI GGTCTC 1 cut(s) 864
BsaJI CCNNGG 6 cut(s) 55, 369, 377, 478, 487, 1017
Bse1I ACTGG 2 cut(s) 554, 832
Bse21I CCTNAGG 1 cut(s) 321
Bse8I GATNNNNATC 1 cut(s) 12
BseBI CCWGG 1 cut(s) 1018
BseDI CCNNGG 6 cut(s) 55, 369, 377, 478, 487, 1017
BseGI GGATG 2 cut(s) 693, 1045
BseJI GATNNNNATC 1 cut(s) 12
BseMII CTCAG 2 cut(s) 528, 1088
BseNI ACTGG 2 cut(s) 554, 832
BseRI GAGGAG 3 cut(s) 56, 65, 175
BseSI GKGCMC 2 cut(s) 486, 1139
BsgI GTGCAG 1 cut(s) 776
Bsh1236I CGCG 2 cut(s) 630, 1061
BshFI GGCC 4 cut(s) 60, 374, 567, 792
BshNI GGYRCC 1 cut(s) 483
BsiHKAI GWGCWC 1 cut(s) 1067
BsiSI CCGG 1 cut(s) 1162
BslFI GGGAC 1 cut(s) 453
BsmAI GTCTC 3 cut(s) 543, 864, 1224
BsmBI CGTCTC 1 cut(s) 543
BsmFI GGGAC 1 cut(s) 453
BsmI GAATGC 1 cut(s) 646
BsnI GGCC 4 cut(s) 60, 374, 567, 792
Bso31I GGTCTC 1 cut(s) 864
Bsp1286I GDGCHC 3 cut(s) 486, 1067, 1139
Bsp143I GATC 4 cut(s) 7, 517, 769, 895
Bsp19I CCATGG 1 cut(s) 369
BspACI CCGC 4 cut(s) 317, 628, 777, 1038
BspANI GGCC 4 cut(s) 60, 374, 567, 792
BspCNI CTCAG 2 cut(s) 527, 1089
BspFNI CGCG 2 cut(s) 630, 1061
BspLI GGNNCC 4 cut(s) 375, 441, 456, 485
BspMI ACCTGC 2 cut(s) 748, 1200
BspPI GGATC 3 cut(s) 15, 512, 764
BspQI GCTCTTC 1 cut(s) 651
BspT107I GGYRCC 1 cut(s) 483
BspTNI GGTCTC 1 cut(s) 864
BsrI ACTGG 2 cut(s) 554, 832
BssECI CCNNGG 6 cut(s) 55, 369, 377, 478, 487, 1017
BssMI GATC 4 cut(s) 7, 517, 769, 895
BssT1I CCWWGG 4 cut(s) 55, 369, 478, 487
Bst2UI CCWGG 1 cut(s) 1018
Bst4CI ACNGT 6 cut(s) 259, 718, 1012, 1078, 1212, 1235
Bst6I CTCTTC 6 cut(s) 136, 154, 193, 651, 707, 1097
BstAPI GCANNNNNTGC 1 cut(s) 961
BstC8I GCNNGC 1 cut(s) 1063
BstDEI CTNAG 4 cut(s) 321, 514, 1097, 1196
BstDSI CCRYGG 1 cut(s) 369
BstF5I GGATG 2 cut(s) 693, 1045
BstFNI CGCG 2 cut(s) 630, 1061
BstKTI GATC 4 cut(s) 10, 520, 772, 898
BstMAI GTCTC 3 cut(s) 543, 864, 1224
BstMBI GATC 4 cut(s) 7, 517, 769, 895
BstMWI GCNNNNNNNGC 2 cut(s) 371, 961
BstNI CCWGG 1 cut(s) 1018
BstSCI CCNGG 2 cut(s) 1016, 1160
BstSLI GKGCMC 2 cut(s) 486, 1139
BstUI CGCG 2 cut(s) 630, 1061
BstX2I RGATCY 1 cut(s) 517
BstYI RGATCY 1 cut(s) 517
Bsu36I CCTNAGG 1 cut(s) 321
BsuI GTATCC 1 cut(s) 375
BsuRI GGCC 4 cut(s) 60, 374, 567, 792
BtgI CCRYGG 1 cut(s) 369
BtsCI GGATG 2 cut(s) 693, 1045
BtsI GCAGTG 2 cut(s) 857, 925
BtsIMutI CAGTG 4 cut(s) 839, 857, 925, 1240
BveI ACCTGC 2 cut(s) 748, 1200
Cac8I GCNNGC 1 cut(s) 1063
Cfr13I GGNCC 4 cut(s) 373, 440, 829, 1040
Csp6I GTAC 2 cut(s) 122, 1057
CviAII CATG 7 cut(s) 274, 337, 370, 424, 563, 610, 690
CviQI GTAC 2 cut(s) 122, 1057
DdeI CTNAG 4 cut(s) 321, 514, 1097, 1196
DpnI GATC 4 cut(s) 9, 519, 771, 897
DpnII GATC 4 cut(s) 7, 517, 769, 895
DriI GACNNNNNGTC 1 cut(s) 243
EaeI YGGCCR 2 cut(s) 565, 790
Eam1104I CTCTTC 6 cut(s) 136, 154, 193, 651, 707, 1097
Eam1105I GACNNNNNGTC 1 cut(s) 243
EarI CTCTTC 6 cut(s) 136, 154, 193, 651, 707, 1097
EciI GGCGGA 1 cut(s) 1053
Eco130I CCWWGG 4 cut(s) 55, 369, 478, 487
Eco31I GGTCTC 1 cut(s) 864
Eco47I GGWCC 3 cut(s) 440, 829, 1040
Eco81I CCTNAGG 1 cut(s) 321
EcoO109I RGGNCCY 1 cut(s) 440
EcoRI GAATTC 2 cut(s) 573, 997
EcoRII CCWGG 1 cut(s) 1016
EcoT14I CCWWGG 4 cut(s) 55, 369, 478, 487
EcoT22I ATGCAT 1 cut(s) 695
ErhI CCWWGG 4 cut(s) 55, 369, 478, 487
Esp3I CGTCTC 1 cut(s) 543
FaeI CATG 7 cut(s) 277, 340, 373, 427, 566, 613, 693
FaqI GGGAC 1 cut(s) 453
FatI CATG 7 cut(s) 273, 336, 369, 423, 562, 609, 689
FauNDI CATATG 1 cut(s) 234
FblI GTMKAC 2 cut(s) 160, 720
Fnu4HI GCNGC 2 cut(s) 317, 628
FokI GGATG 2 cut(s) 680, 1032
Fsp4HI GCNGC 2 cut(s) 317, 628
FspBI CTAG 1 cut(s) 1032
GluI GCNGC 2 cut(s) 317, 628
GsuI CTGGAG 1 cut(s) 379
HaeIII GGCC 4 cut(s) 60, 374, 567, 792
HapII CCGG 1 cut(s) 1162
Hin1II CATG 7 cut(s) 277, 340, 373, 427, 566, 613, 693
HinfI GANTC 4 cut(s) 117, 146, 533, 686
HpaII CCGG 1 cut(s) 1162
HphI GGTGA 1 cut(s) 533
Hpy166II GTNNAC 4 cut(s) 161, 511, 607, 721
Hpy188III TCNNGA 5 cut(s) 262, 358, 430, 650, 919
Hpy8I GTNNAC 4 cut(s) 161, 511, 607, 721
HpyAV CCTTC 2 cut(s) 33, 1125
HpyCH4III ACNGT 6 cut(s) 259, 718, 1012, 1078, 1212, 1235
HpyCH4V TGCA 6 cut(s) 693, 757, 930, 947, 1205, 1219
HpyF10VI GCNNNNNNNGC 2 cut(s) 371, 961
HpyF3I CTNAG 4 cut(s) 321, 514, 1097, 1196
Hsp92II CATG 7 cut(s) 277, 340, 373, 427, 566, 613, 693
Kzo9I GATC 4 cut(s) 7, 517, 769, 895
LguI GCTCTTC 1 cut(s) 651
LmnI GCTCC 1 cut(s) 460
LweI GCATC 1 cut(s) 702
MaeI CTAG 1 cut(s) 1032
MaeIII GTNAC 4 cut(s) 259, 1012, 1072, 1106
MalI GATC 4 cut(s) 9, 519, 771, 897
MboI GATC 4 cut(s) 7, 517, 769, 895
MflI RGATCY 1 cut(s) 517
MhlI GDGCHC 3 cut(s) 486, 1067, 1139
MluCI AATT 9 cut(s) 240, 296, 350, 573, 633, 706, 997, 1177, 1220
MlyI GAGTC 1 cut(s) 140
Mph1103I ATGCAT 1 cut(s) 695
MroXI GAANNNNTTC 1 cut(s) 473
MseI TTAA 1 cut(s) 1214
MspI CCGG 1 cut(s) 1162
MspR9I CCNGG 2 cut(s) 1018, 1162
Mva1269I GAATGC 1 cut(s) 646
MvaI CCWGG 1 cut(s) 1018
MvnI CGCG 2 cut(s) 630, 1061
MwoI GCNNNNNNNGC 2 cut(s) 371, 961
NciI CCSGG 1 cut(s) 1162
NcoI CCATGG 1 cut(s) 369
NdeI CATATG 1 cut(s) 234
NdeII GATC 4 cut(s) 7, 517, 769, 895
NlaIII CATG 7 cut(s) 277, 340, 373, 427, 566, 613, 693
NlaIV GGNNCC 4 cut(s) 375, 441, 456, 485
NmeAIII GCCGAG 1 cut(s) 646
NmuCI GTSAC 3 cut(s) 259, 1012, 1072
NsiI ATGCAT 1 cut(s) 695
PciSI GCTCTTC 1 cut(s) 651
PcsI WCGNNNNNNNCGW 1 cut(s) 339
PctI GAATGC 1 cut(s) 646
PdmI GAANNNNTTC 1 cut(s) 473
PfeI GAWTC 3 cut(s) 117, 533, 686
PflFI GACNNNGTC 1 cut(s) 257
PkrI GCNGC 2 cut(s) 318, 629
PleI GAGTC 1 cut(s) 140
PpsI GAGTC 1 cut(s) 140
PpuMI RGGWCCY 1 cut(s) 440
PsiI TTATAA 1 cut(s) 528
Psp5II RGGWCCY 1 cut(s) 440
Psp6I CCWGG 1 cut(s) 1016
PspGI CCWGG 1 cut(s) 1016
PspN4I GGNNCC 4 cut(s) 375, 441, 456, 485
PspPI GGNCC 4 cut(s) 373, 440, 829, 1040
PspPPI RGGWCCY 1 cut(s) 440
PsuI RGATCY 1 cut(s) 517
PsyI GACNNNGTC 1 cut(s) 257
RsaI GTAC 2 cut(s) 123, 1058
RsaNI GTAC 2 cut(s) 122, 1057
SapI GCTCTTC 1 cut(s) 651
SaqAI TTAA 1 cut(s) 1214
SatI GCNGC 2 cut(s) 317, 628
Sau3AI GATC 4 cut(s) 7, 517, 769, 895
Sau96I GGNCC 4 cut(s) 373, 440, 829, 1040
SchI GAGTC 1 cut(s) 140
ScrFI CCNGG 2 cut(s) 1018, 1162
SduI GDGCHC 3 cut(s) 486, 1067, 1139
SfaNI GCATC 1 cut(s) 702
SinI GGWCC 3 cut(s) 440, 829, 1040
Sse9I AATT 9 cut(s) 240, 296, 350, 573, 633, 706, 997, 1177, 1220
SsiI CCGC 4 cut(s) 317, 628, 777, 1038
SspI AATATT 1 cut(s) 226
SspMI CTAG 1 cut(s) 1032
StyD4I CCNGG 2 cut(s) 1016, 1160
StyI CCWWGG 4 cut(s) 55, 369, 478, 487
TaaI ACNGT 6 cut(s) 259, 718, 1012, 1078, 1212, 1235
TaqI TCGA 2 cut(s) 651, 1053
TaqII GACCGA 1 cut(s) 293
TasI AATT 9 cut(s) 240, 296, 350, 573, 633, 706, 997, 1177, 1220
TauI GCSGC 2 cut(s) 319, 630
TfiI GAWTC 3 cut(s) 117, 533, 686
Tru1I TTAA 1 cut(s) 1214
Tru9I TTAA 1 cut(s) 1214
TscAI CASTG 4 cut(s) 839, 857, 932, 1240
TseFI GTSAC 3 cut(s) 259, 1012, 1072
Tsp45I GTSAC 3 cut(s) 259, 1012, 1072
TspDTI ATGAA 9 cut(s) 17, 189, 275, 412, 437, 546, 551, 816, 989
TspGWI ACGGA 3 cut(s) 198, 384, 510
TspRI CASTG 4 cut(s) 839, 857, 932, 1240
Tth111I GACNNNGTC 1 cut(s) 257
VpaK11BI GGWCC 3 cut(s) 440, 829, 1040
XapI RAATTY 3 cut(s) 573, 997, 1177
XmiI GTMKAC 2 cut(s) 160, 720
XmnI GAANNNNTTC 1 cut(s) 473
XspI CTAG 1 cut(s) 1032
Zsp2I ATGCAT 1 cut(s) 695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.