Rorug04G0358000

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001217
Physical Location & Seq
Forward (+)
53109999 .. 53113170
3172 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0358000.1

Sequence Viewer

Length: 2019 bp
ATGGCAGCCAAGGCCTTCTTCTTCTTTCTCTCTCTACTATTTCTCATTATCTCTCTAATCCACGCAACTTCAAACCCAGATTCCGAGCCTCTCTTAGCGTTCAAGGCCACGTCGGACGCTTCCAATGCTCTCGCCACGTGGAACTCATCCTCCGACCCGTGCACCACCTGGGTCGGAGTCTCCTGCACCCGGAGCCGGGTCTCGCGGCTCGTTCTCGAGAACCTCGGCCTTAGAGGCTCGTTCGCGGCGCTGACTGGGTTGACTCAGCTCCGAGTTCTCAGCCTGAAGCGGAACCGCCTCTCCGGCCCCATTCCCGACCTCTCTAACCTCAATTCCCTCAAGCTCCTCTTCCTCTCCTACAACGACTTCTCCGGCGACTTTCCCGCTTCCGTAACGTCCCTTTTCCGGCTTTACCGCCTCGATCTCGCTTACAACAACTTGTCCGGTGAAATTCCCGAGACGGTCAACCATTTGACTCATCTCCTCACTCTCCGCCTCGAAGCGAACCGATTTTCCGGCTCGATTTCCGGCCTCAACATCCCTAACCTCCAGGAGTTCAACGTCTCGGCGAACCGTTTGTCCGGCGAAATTCCCAAGTCGTTGTCTATTTTCTCCGAGTCTGCATTTGCGCAAAACCCGGGTCTGTGCGGGTCTCCGGTTCAGACCTGTAAAACAGAAACCGACCCTACTCGACCCGGAGGAGCTATTGCGTCTCCTGTAATACCCGCCGGAAACCCAACCGTAGTGGCGTCCTCGCCGACTTCATTGCCATCAAGCTCTACGCCCACCAAATCGGAAAAGTCGACGTCCCACCACAACGGAGGTTCCAGAATTAGTCCGGCGGCTCTGATCGCCATAATAGTGGGCGACGTGTTGGTCCTCTTTTTGGTCTCGCTGCTGCTCTACTGCTACTTCTGGCGGAACTTCGCGGCCAAAATGCGGCAGGGCAAGAGCAGCTCCAAGCTTCTGGAGACAGAGAAGATAGTCTACTCGTCGAGCCCATACTCGGCCCAGCCCTCTTTCGAGAGGGGACAGATGGTGTTTTTCGAAGGCGTGAAGCGTTTCGAGCTGGAGGACTTGCTGAGAGCCTCGGCGGAGATGTTGGGGAAAGGTGGGTTCGGGACGGCGTACAAGGCGGTTTTGGACGACGGCAATGTTGTGGCGGTGAAGAGGCTCAAGGATGCTCAAATTGGTGGGAAGAATCAGTTTGAGCAGCACATGGCGGTGCTGGGCAAGCTTAACCATCTCAACGTTGTGAGCTTGCGGGCTTATTATTTCGCTAGGGAGGAGAAGTTGCTGGTCTACGATTACATGCCAAATGGAAGCTTGTTTTGGCTTCTTCATGGTAACAGAGGACCTGGAAGAACCCCACTGGACTGGACCACCAGACTGAAAATTGCAGCCGGAGCTGCACGAGGGCTAGGTTTCATTCACAGCTCCTGCGCTCCCCTTAAGCTCACCCACGGCAACATCAAATCCACCAACATTCTCTTGGACAAGACCGGCAATGCTCGTGTCTCCGACTTCGGACTTTCGGCTTTTTCGCCTTCCTCTTCCGCCGCGTCGGCGCCGCGGTCGTGCGGGTACCGAGCTCCGGAGTTGCTGGAGGGTCGGAAAGTAACGCAGAAGTCGGACGTGTATGCTTTCGGTGTTCTTTTGCTGGAGATATTGACCGGGAAATGCCCGTCCGCGGTCGTGGATGGCGGGTCCGGCGGTGGCTACGGCGGACTGGTGGACCTGCCGAGGTGGGTCCAGTCTGTGGTGAGAGAGGAGTGGACTGCCGAGGTGTTTGACTTGGAGTTGATGAGGTACAAGGACATTGAGGAGGAGATGGTGGGGCTTTTGCAGGTTGCAATGGCTTGCACAACCGCAGTACCTGATCAACGGCCGAGGATGAGCCAGGTGGTGAAGATGATCGACGAGATTCGAGGCGTGGCAGGGTCACCGCCTCATGAGGCGTGTGATTCCATGACCGAGTCGCCGGCTTTGTCCGAGGACACTTGCGGAGCAAGTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

672

Amino Acids

72.58

Weight (kDa)

8.5

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 25 - 63 1.4e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 84 - 176 2.1e-06 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 366 - 638 2.8e-36 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 366 - 565 9.7e-36 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 875
AatII GACGTC 1 cut(s) 809
Acc16I TGCGCA 1 cut(s) 630
Acc36I ACCTGC 2 cut(s) 1746, 1837
Acc65I GGTACC 1 cut(s) 1584
AccB1I GGYRCC 2 cut(s) 1567, 1584
AccB7I CCANNNNNTGG 1 cut(s) 1759
AccI GTMKAC 3 cut(s) 803, 987, 1302
AccII CGCG 6 cut(s) 205, 245, 929, 1562, 1573, 1691
AccIII TCCGGA 1 cut(s) 1594
AclI AACGTT 1 cut(s) 1251
AcoI YGGCCR 2 cut(s) 930, 1886
AcsI RAATTY 2 cut(s) 450, 588
AcuI CTGAAG 1 cut(s) 305
AcvI CACGTG 1 cut(s) 138
AcyI GRCGYC 3 cut(s) 749, 806, 1568
AfaI GTAC 4 cut(s) 1130, 1586, 1811, 1875
AflII CTTAAG 1 cut(s) 1451
AflIII ACRYGT 2 cut(s) 870, 1635
AgsI TTSAA 3 cut(s) 72, 103, 559
AjiI CACGTC 3 cut(s) 111, 871, 1636
AjnI CCWGG 4 cut(s) 167, 549, 1357, 1899
AjuI GAANNNNNNNTTGG 3 cut(s) 34, 1315, 1347
AloI GAACNNNNNNTCC 8 cut(s) 134, 166, 284, 316, 497, 529, 563, 595
Alw21I GWGCWC 2 cut(s) 164, 1594
Alw26I GTCTC 9 cut(s) 184, 205, 452, 568, 657, 717, 895, 965, 1522
Alw44I GTGCAC 1 cut(s) 160
AlwNI CAGNNNCTG 2 cut(s) 1440, 1877
Ama87I CYCGRG 3 cut(s) 215, 455, 637
Aor13HI TCCGGA 1 cut(s) 1594
AoxI GGCC 8 cut(s) 12, 105, 226, 304, 529, 930, 1008, 1886
ApaLI GTGCAC 1 cut(s) 160
ApeKI GCWGC 7 cut(s) 5, 895, 898, 954, 1213, 1400, 1409
ApoI RAATTY 2 cut(s) 450, 588
ArsI GACNNNNNNTTYG 4 cut(s) 563, 595, 625, 657
Asp700I GAANNNNTTC 1 cut(s) 1061
Asp718I GGTACC 1 cut(s) 1584
AspLEI GCGC 4 cut(s) 250, 631, 1445, 1570
AspS9I GGNCC 8 cut(s) 305, 877, 1009, 1355, 1380, 1707, 1735, 1750
AsuC2I CCSGG 6 cut(s) 190, 197, 638, 639, 696, 1675
AsuHPI GGTGA 6 cut(s) 458, 1177, 1450, 1774, 1918, 1935
AsuII TTCGAA 1 cut(s) 1047
AvaI CYCGRG 3 cut(s) 215, 455, 637
AvaII GGWCC 6 cut(s) 877, 1355, 1380, 1707, 1735, 1750
BaeGI GKGCMC 1 cut(s) 164
BaeI ACNNNNGTAYC 2 cut(s) 1857, 1890
BanI GGYRCC 2 cut(s) 1567, 1584
BanII GRGCYC 2 cut(s) 1001, 1594
BarI GAAGNNNNNNTAC 2 cut(s) 971, 1003
BauI CACGAG 2 cut(s) 1413, 1512
BbrPI CACGTG 1 cut(s) 138
Bbv12I GWGCWC 2 cut(s) 164, 1594
BbvI GCAGC 7 cut(s) 17, 882, 885, 966, 1225, 1396, 1412
BccI CCATC 5 cut(s) 778, 1030, 1251, 1694, 1825
BceAI ACGGC 5 cut(s) 1140, 1165, 1480, 1738, 1901
BcgI CGANNNNNNTGC 2 cut(s) 748, 782
BciT130I CCWGG 4 cut(s) 169, 551, 1359, 1901
BclI TGATCA 1 cut(s) 1879
BcnI CCSGG 6 cut(s) 190, 197, 638, 639, 696, 1675
BcoDI GTCTC 9 cut(s) 184, 205, 452, 568, 657, 717, 895, 965, 1522
BfaI CTAG 2 cut(s) 1281, 1421
BfoI RGCGCY 2 cut(s) 251, 1571
BfrI CTTAAG 1 cut(s) 1451
BfuAI ACCTGC 2 cut(s) 1746, 1837
BglI GCCNNNNNGGC 3 cut(s) 234, 303, 1565
Bme18I GGWCC 6 cut(s) 877, 1355, 1380, 1707, 1735, 1750
BmeT110I CYCGRG 3 cut(s) 215, 455, 637
BmgBI CACGTC 3 cut(s) 111, 871, 1636
BmgT120I GGNCC 8 cut(s) 305, 877, 1009, 1355, 1380, 1707, 1735, 1750
BmiI GGNNCC 8 cut(s) 194, 293, 307, 826, 1569, 1586, 1708, 1751
BmrI ACTGGG 1 cut(s) 264
BmsI GCATC 1 cut(s) 1171
BmuI ACTGGG 1 cut(s) 264
BpmI CTGGAG 5 cut(s) 533, 989, 1091, 1625, 1682
Bpu14I TTCGAA 1 cut(s) 1047
BpuEI CTTGAG 2 cut(s) 323, 1160
BpuMI CCSGG 6 cut(s) 190, 197, 638, 639, 696, 1675
BsaAI YACGTR 1 cut(s) 138
BsaHI GRCGYC 3 cut(s) 749, 806, 1568
BsaI GGTCTC 3 cut(s) 205, 657, 895
BsaWI WCCGGW 3 cut(s) 443, 655, 1594
BsaXI ACNNNNNCTCC 8 cut(s) 134, 164, 284, 314, 353, 383, 1277, 1307
Bse118I RCCGGY 2 cut(s) 1502, 1981
Bse1I ACTGG 5 cut(s) 259, 1377, 1382, 1734, 1753
Bse3DI GCAATG 4 cut(s) 764, 1159, 1513, 1860
BseAI TCCGGA 1 cut(s) 1594
BseBI CCWGG 4 cut(s) 169, 551, 1359, 1901
BseGI GGATG 5 cut(s) 146, 537, 1186, 1705, 1899
BseMI GCAATG 4 cut(s) 764, 1159, 1513, 1860
BseMII CTCAG 3 cut(s) 278, 292, 1073
BseNI ACTGG 5 cut(s) 259, 1377, 1382, 1734, 1753
BseRI GAGGAG 7 cut(s) 335, 473, 714, 1301, 1784, 1838, 1841
BseSI GKGCMC 1 cut(s) 164
BseX3I CGGCCG 1 cut(s) 1886
BseXI GCAGC 7 cut(s) 17, 882, 885, 966, 1225, 1396, 1412
BseYI CCCAGC 2 cut(s) 1011, 1228
BsgI GTGCAG 2 cut(s) 169, 1395
Bsh1236I CGCG 6 cut(s) 205, 245, 929, 1562, 1573, 1691
Bsh1285I CGRYCG 3 cut(s) 1577, 1695, 1889
BshFI GGCC 8 cut(s) 14, 107, 228, 306, 531, 932, 1010, 1888
BshNI GGYRCC 2 cut(s) 1567, 1584
BsiEI CGRYCG 3 cut(s) 1577, 1695, 1889
BsiHKAI GWGCWC 2 cut(s) 164, 1594
BsiHKCI CYCGRG 3 cut(s) 215, 455, 637
BslFI GGGAC 4 cut(s) 382, 793, 1044, 1135
BsmAI GTCTC 9 cut(s) 184, 205, 452, 568, 657, 717, 895, 965, 1522
BsmBI CGTCTC 3 cut(s) 452, 568, 717
BsmFI GGGAC 4 cut(s) 382, 793, 1044, 1135
BsnI GGCC 8 cut(s) 14, 107, 228, 306, 531, 932, 1010, 1888
Bso31I GGTCTC 3 cut(s) 205, 657, 895
BsoBI CYCGRG 3 cut(s) 215, 455, 637
Bsp119I TTCGAA 1 cut(s) 1047
Bsp1286I GDGCHC 3 cut(s) 164, 1001, 1594
Bsp13I TCCGGA 1 cut(s) 1594
Bsp143I GATC 4 cut(s) 421, 849, 1879, 1914
BspANI GGCC 8 cut(s) 14, 107, 228, 306, 531, 932, 1010, 1888
BspCNI CTCAG 3 cut(s) 277, 291, 1074
BspEI TCCGGA 1 cut(s) 1594
BspFNI CGCG 6 cut(s) 205, 245, 929, 1562, 1573, 1691
BspHI TCATGA 1 cut(s) 1951
BspLI GGNNCC 8 cut(s) 194, 293, 307, 826, 1569, 1586, 1708, 1751
BspMI ACCTGC 2 cut(s) 1746, 1837
BspT104I TTCGAA 1 cut(s) 1047
BspT107I GGYRCC 2 cut(s) 1567, 1584
BspTI CTTAAG 1 cut(s) 1451
BspTNI GGTCTC 3 cut(s) 205, 657, 895
BsrDI GCAATG 4 cut(s) 764, 1159, 1513, 1860
BsrFI RCCGGY 2 cut(s) 1502, 1981
BsrI ACTGG 5 cut(s) 259, 1377, 1382, 1734, 1753
BssAI RCCGGY 2 cut(s) 1502, 1981
BssMI GATC 4 cut(s) 421, 849, 1879, 1914
BssNI GRCGYC 3 cut(s) 749, 806, 1568
BssSI CACGAG 2 cut(s) 1413, 1512
BssT1I CCWWGG 1 cut(s) 9
Bst2BI CACGAG 2 cut(s) 1413, 1512
Bst2UI CCWGG 4 cut(s) 169, 551, 1359, 1901
Bst4CI ACNGT 3 cut(s) 463, 575, 742
Bst6I CTCTTC 3 cut(s) 353, 1163, 1558
BstACI GRCGYC 3 cut(s) 749, 806, 1568
BstAFI CTTAAG 1 cut(s) 1451
BstBAI YACGTR 1 cut(s) 138
BstBI TTCGAA 1 cut(s) 1047
BstC8I GCNNGC 5 cut(s) 1235, 1262, 1266, 1861, 1983
BstDEI CTNAG 5 cut(s) 94, 230, 264, 278, 1082
BstDSI CCRYGG 3 cut(s) 1462, 1571, 1689
BstEII GGTNACC 1 cut(s) 1941
BstF5I GGATG 5 cut(s) 146, 537, 1186, 1705, 1899
BstFNI CGCG 6 cut(s) 205, 245, 929, 1562, 1573, 1691
BstH2I RGCGCY 2 cut(s) 251, 1571
BstHHI GCGC 4 cut(s) 250, 631, 1445, 1570
BstKTI GATC 4 cut(s) 424, 852, 1882, 1917
BstMAI GTCTC 9 cut(s) 184, 205, 452, 568, 657, 717, 895, 965, 1522
BstMBI GATC 4 cut(s) 421, 849, 1879, 1914
BstMCI CGRYCG 3 cut(s) 1577, 1695, 1889
BstNI CCWGG 4 cut(s) 169, 551, 1359, 1901
BstNSI RCATGY 1 cut(s) 1315
BstPI GGTNACC 1 cut(s) 1941
BstSLI GKGCMC 1 cut(s) 164
BstUI CGCG 6 cut(s) 205, 245, 929, 1562, 1573, 1691
BstV1I GCAGC 7 cut(s) 17, 882, 885, 966, 1225, 1396, 1412
BstXI CCANNNNNNTGG 3 cut(s) 862, 967, 1377
BstZI CGGCCG 1 cut(s) 1886
BsuRI GGCC 8 cut(s) 14, 107, 228, 306, 531, 932, 1010, 1888
BtgI CCRYGG 3 cut(s) 1462, 1571, 1689
BtrI CACGTC 3 cut(s) 111, 871, 1636
BtsCI GGATG 5 cut(s) 146, 537, 1186, 1705, 1899
BtsIMutI CAGTG 1 cut(s) 1370
BveI ACCTGC 2 cut(s) 1746, 1837
Cac8I GCNNGC 5 cut(s) 1235, 1262, 1266, 1861, 1983
CaiI CAGNNNCTG 2 cut(s) 1440, 1877
CciI TCATGA 1 cut(s) 1951
CfoI GCGC 4 cut(s) 250, 631, 1445, 1570
Cfr10I RCCGGY 2 cut(s) 1502, 1981
Cfr13I GGNCC 8 cut(s) 305, 877, 1009, 1355, 1380, 1707, 1735, 1750
Cfr42I CCGCGG 2 cut(s) 1574, 1692
Cfr9I CCCGGG 1 cut(s) 637
CseI GACGC 4 cut(s) 125, 699, 738, 1551
Csp6I GTAC 4 cut(s) 1129, 1585, 1810, 1874
CspCI CAANNNNNGTGG 2 cut(s) 726, 761
CviAII CATG 5 cut(s) 1219, 1312, 1343, 1952, 1969
CviQI GTAC 4 cut(s) 1129, 1585, 1810, 1874
DdeI CTNAG 5 cut(s) 94, 230, 264, 278, 1082
DinI GGCGCC 1 cut(s) 1569
DpnI GATC 4 cut(s) 423, 851, 1881, 1916
DpnII GATC 4 cut(s) 421, 849, 1879, 1914
DrdI GACNNNNNNGTC 1 cut(s) 875
DseDI GACNNNNNNGTC 1 cut(s) 875
EaeI YGGCCR 2 cut(s) 930, 1886
EagI CGGCCG 1 cut(s) 1886
Eam1104I CTCTTC 3 cut(s) 353, 1163, 1558
EarI CTCTTC 3 cut(s) 353, 1163, 1558
EciI GGCGGA 5 cut(s) 482, 934, 1109, 1546, 1740
Ecl136II GAGCTC 1 cut(s) 1592
EclXI CGGCCG 1 cut(s) 1886
Eco130I CCWWGG 1 cut(s) 9
Eco147I AGGCCT 1 cut(s) 14
Eco24I GRGCYC 2 cut(s) 1001, 1594
Eco31I GGTCTC 3 cut(s) 205, 657, 895
Eco47I GGWCC 6 cut(s) 877, 1355, 1380, 1707, 1735, 1750
Eco52I CGGCCG 1 cut(s) 1886
Eco53kI GAGCTC 1 cut(s) 1592
Eco57I CTGAAG 1 cut(s) 305
Eco72I CACGTG 1 cut(s) 138
Eco88I CYCGRG 3 cut(s) 215, 455, 637
Eco91I GGTNACC 1 cut(s) 1941
EcoICRI GAGCTC 1 cut(s) 1592
EcoO109I RGGNCCY 1 cut(s) 1355
EcoO65I GGTNACC 1 cut(s) 1941
EcoRII CCWGG 4 cut(s) 167, 549, 1357, 1899
EcoT14I CCWWGG 1 cut(s) 9
EcoT38I GRGCYC 2 cut(s) 1001, 1594
EgeI GGCGCC 1 cut(s) 1569
EheI GGCGCC 1 cut(s) 1569
ErhI CCWWGG 1 cut(s) 9
Esp3I CGTCTC 3 cut(s) 452, 568, 717
FaeI CATG 5 cut(s) 1222, 1315, 1346, 1955, 1972
FaiI YATR 8 cut(s) 857, 1003, 1220, 1313, 1344, 1641, 1953, 1970
FalI AAGNNNNNCTT 3 cut(s) 34, 332, 364
FaqI GGGAC 4 cut(s) 382, 793, 1044, 1135
FatI CATG 5 cut(s) 1218, 1311, 1342, 1951, 1968
FauI CCCGC 6 cut(s) 391, 641, 733, 1257, 1574, 1697
FbaI TGATCA 1 cut(s) 1879
FblI GTMKAC 3 cut(s) 803, 987, 1302
FokI GGATG 5 cut(s) 133, 524, 1193, 1712, 1906
FriOI GRGCYC 2 cut(s) 1001, 1594
FspBI CTAG 2 cut(s) 1281, 1421
FspI TGCGCA 1 cut(s) 630
GlaI GCGC 4 cut(s) 249, 630, 1444, 1569
GsaI CCCAGC 2 cut(s) 1015, 1232
GsuI CTGGAG 5 cut(s) 533, 989, 1091, 1625, 1682
HaeII RGCGCY 2 cut(s) 251, 1571
HaeIII GGCC 8 cut(s) 14, 107, 228, 306, 531, 932, 1010, 1888
HgaI GACGC 4 cut(s) 125, 699, 738, 1551
HhaI GCGC 4 cut(s) 250, 631, 1445, 1570
Hin1I GRCGYC 3 cut(s) 749, 806, 1568
Hin1II CATG 5 cut(s) 1222, 1315, 1346, 1955, 1972
Hin6I GCGC 4 cut(s) 248, 629, 1443, 1568
HinP1I GCGC 4 cut(s) 248, 629, 1443, 1568
HincII GTYRAC 3 cut(s) 261, 466, 804
HindII GTYRAC 3 cut(s) 261, 466, 804
HindIII AAGCTT 3 cut(s) 962, 1235, 1324
HinfI GANTC 9 cut(s) 80, 177, 262, 475, 617, 1201, 1924, 1964, 1976
HphI GGTGA 6 cut(s) 458, 1177, 1450, 1774, 1918, 1935
Hpy166II GTNNAC 8 cut(s) 162, 261, 466, 804, 988, 1303, 1735, 1776
Hpy8I GTNNAC 8 cut(s) 162, 261, 466, 804, 988, 1303, 1735, 1776
Hpy99I CGWCG 7 cut(s) 115, 808, 872, 997, 1151, 1567, 1922
HpyAV CCTTC 3 cut(s) 25, 1043, 1557
HpyCH4III ACNGT 3 cut(s) 463, 575, 742
HpyCH4IV ACGT 8 cut(s) 110, 137, 395, 561, 806, 870, 1251, 1635
HpyCH4V TGCA 8 cut(s) 162, 186, 623, 1400, 1412, 1846, 1853, 1863
HpyF3I CTNAG 5 cut(s) 94, 230, 264, 278, 1082
HpySE526I ACGT 8 cut(s) 110, 137, 395, 561, 806, 870, 1251, 1635
Hsp92I GRCGYC 3 cut(s) 749, 806, 1568
Hsp92II CATG 5 cut(s) 1222, 1315, 1346, 1955, 1972
HspAI GCGC 4 cut(s) 248, 629, 1443, 1568
KasI GGCGCC 1 cut(s) 1567
Kpn2I TCCGGA 1 cut(s) 1594
KpnI GGTACC 1 cut(s) 1588
KroI GCCGGC 1 cut(s) 1981
KroNI GCCGGC 1 cut(s) 1983
Ksp22I TGATCA 1 cut(s) 1879
KspI CCGCGG 2 cut(s) 1574, 1692
Kzo9I GATC 4 cut(s) 421, 849, 1879, 1914
Lsp1109I GCAGC 7 cut(s) 17, 882, 885, 966, 1225, 1396, 1412
LweI GCATC 1 cut(s) 1171
MaeI CTAG 2 cut(s) 1281, 1421
MaeII ACGT 8 cut(s) 110, 137, 395, 561, 806, 870, 1251, 1635
MaeIII GTNAC 4 cut(s) 391, 1346, 1618, 1941
MalI GATC 4 cut(s) 423, 851, 1881, 1916
MboI GATC 4 cut(s) 421, 849, 1879, 1914
MhlI GDGCHC 3 cut(s) 164, 1001, 1594
MluCI AATT 6 cut(s) 331, 450, 588, 831, 1188, 1395
Mly113I GGCGCC 1 cut(s) 1568
MlyI GAGTC 5 cut(s) 186, 256, 469, 626, 1985
MmeI TCCRAC 6 cut(s) 93, 154, 177, 1545, 1592, 1611
MroI TCCGGA 1 cut(s) 1594
MroNI GCCGGC 1 cut(s) 1981
MroXI GAANNNNTTC 1 cut(s) 1061
MseI TTAA 2 cut(s) 1239, 1452
MslI CAYNNNNRTG 2 cut(s) 860, 1223
MspA1I CMGCKG 2 cut(s) 1573, 1691
MspCI CTTAAG 1 cut(s) 1451
MvaI CCWGG 4 cut(s) 169, 551, 1359, 1901
MvnI CGCG 6 cut(s) 205, 245, 929, 1562, 1573, 1691
NaeI GCCGGC 1 cut(s) 1983
NarI GGCGCC 1 cut(s) 1568
NciI CCSGG 6 cut(s) 190, 197, 638, 639, 696, 1675
NdeII GATC 4 cut(s) 421, 849, 1879, 1914
NgoMIV GCCGGC 1 cut(s) 1981
NlaIII CATG 5 cut(s) 1222, 1315, 1346, 1955, 1972
NlaIV GGNNCC 8 cut(s) 194, 293, 307, 826, 1569, 1586, 1708, 1751
NmeAIII GCCGAG 7 cut(s) 204, 545, 986, 1070, 1767, 1807, 1914
NmuCI GTSAC 1 cut(s) 1941
NsbI TGCGCA 1 cut(s) 630
NspI RCATGY 1 cut(s) 1315
NspV TTCGAA 1 cut(s) 1047
PaeR7I CTCGAG 1 cut(s) 215
PagI TCATGA 1 cut(s) 1951
PceI AGGCCT 1 cut(s) 14
PcsI WCGNNNNNNNCGW 1 cut(s) 800
PdiI GCCGGC 1 cut(s) 1983
PdmI GAANNNNTTC 1 cut(s) 1061
PfeI GAWTC 4 cut(s) 80, 1201, 1924, 1964
PflFI GACNNNGTC 1 cut(s) 1975
PflMI CCANNNNNTGG 1 cut(s) 1759
PfoI TCCNGGA 1 cut(s) 549
PleI GAGTC 5 cut(s) 185, 256, 469, 625, 1984
PluTI GGCGCC 1 cut(s) 1571
PmaCI CACGTG 1 cut(s) 138
PmlI CACGTG 1 cut(s) 138
PpsI GAGTC 5 cut(s) 185, 256, 469, 625, 1984
Ppu21I YACGTR 1 cut(s) 138
PpuMI RGGWCCY 1 cut(s) 1355
Psp124BI GAGCTC 1 cut(s) 1594
Psp1406I AACGTT 1 cut(s) 1251
Psp5II RGGWCCY 1 cut(s) 1355
Psp6I CCWGG 4 cut(s) 167, 549, 1357, 1899
PspCI CACGTG 1 cut(s) 138
PspEI GGTNACC 1 cut(s) 1941
PspFI CCCAGC 2 cut(s) 1011, 1228
PspGI CCWGG 4 cut(s) 167, 549, 1357, 1899
PspN4I GGNNCC 8 cut(s) 194, 293, 307, 826, 1569, 1586, 1708, 1751
PspPI GGNCC 8 cut(s) 305, 877, 1009, 1355, 1380, 1707, 1735, 1750
PspPPI RGGWCCY 1 cut(s) 1355
PstNI CAGNNNCTG 2 cut(s) 1440, 1877
PsyI GACNNNGTC 1 cut(s) 1975
RsaI GTAC 4 cut(s) 1130, 1586, 1811, 1875
RsaNI GTAC 4 cut(s) 1129, 1585, 1810, 1874
RseI CAYNNNNRTG 2 cut(s) 860, 1223
SacI GAGCTC 1 cut(s) 1594
SacII CCGCGG 2 cut(s) 1574, 1692
SalI GTCGAC 1 cut(s) 802
SaqAI TTAA 2 cut(s) 1239, 1452
Sau3AI GATC 4 cut(s) 421, 849, 1879, 1914
Sau96I GGNCC 8 cut(s) 305, 877, 1009, 1355, 1380, 1707, 1735, 1750
SchI GAGTC 5 cut(s) 186, 256, 469, 626, 1985
SduI GDGCHC 3 cut(s) 164, 1001, 1594
SfaNI GCATC 1 cut(s) 1171
SfoI GGCGCC 1 cut(s) 1569
Sfr274I CTCGAG 1 cut(s) 215
Sfr303I CCGCGG 2 cut(s) 1574, 1692
SfuI TTCGAA 1 cut(s) 1047
SgrBI CCGCGG 2 cut(s) 1574, 1692
SinI GGWCC 6 cut(s) 877, 1355, 1380, 1707, 1735, 1750
SlaI CTCGAG 1 cut(s) 215
SmaI CCCGGG 1 cut(s) 639
SmiMI CAYNNNNRTG 2 cut(s) 860, 1223
SmlI CTYRAG 4 cut(s) 215, 338, 1175, 1451
SmoI CTYRAG 4 cut(s) 215, 338, 1175, 1451
Sse9I AATT 6 cut(s) 331, 450, 588, 831, 1188, 1395
SseBI AGGCCT 1 cut(s) 14
SspDI GGCGCC 1 cut(s) 1567
SspMI CTAG 2 cut(s) 1281, 1421
SstI GAGCTC 1 cut(s) 1594
StuI AGGCCT 1 cut(s) 14
StyI CCWWGG 1 cut(s) 9
TaaI ACNGT 3 cut(s) 463, 575, 742
TaiI ACGT 8 cut(s) 113, 140, 398, 564, 809, 873, 1254, 1638
TaqII GACCGA 1 cut(s) 1988
TasI AATT 6 cut(s) 331, 450, 588, 831, 1188, 1395
TauI GCSGC 7 cut(s) 208, 248, 845, 932, 943, 1562, 1573
TfiI GAWTC 4 cut(s) 80, 1201, 1924, 1964
Tru1I TTAA 2 cut(s) 1239, 1452
Tru9I TTAA 2 cut(s) 1239, 1452
TscAI CASTG 1 cut(s) 1377
TseFI GTSAC 1 cut(s) 1941
TseI GCWGC 7 cut(s) 5, 895, 898, 954, 1213, 1400, 1409
Tsp45I GTSAC 1 cut(s) 1941
TspDTI ATGAA 3 cut(s) 753, 1331, 1417
TspGWI ACGGA 2 cut(s) 379, 834
TspMI CCCGGG 1 cut(s) 637
TspRI CASTG 1 cut(s) 1377
Tth111I GACNNNGTC 1 cut(s) 1975
Van91I CCANNNNNTGG 1 cut(s) 1759
Vha464I CTTAAG 1 cut(s) 1451
VneI GTGCAC 1 cut(s) 160
VpaK11BI GGWCC 6 cut(s) 877, 1355, 1380, 1707, 1735, 1750
XapI RAATTY 2 cut(s) 450, 588
XceI RCATGY 1 cut(s) 1315
XcmI CCANNNNNNNNNTGG 1 cut(s) 1489
XhoI CTCGAG 1 cut(s) 215
XmaI CCCGGG 1 cut(s) 637
XmiI GTMKAC 3 cut(s) 803, 987, 1302
XmnI GAANNNNTTC 1 cut(s) 1061
XspI CTAG 2 cut(s) 1281, 1421
ZraI GACGTC 1 cut(s) 807
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.