Rh4DG427000

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001217
Physical Location & Seq
Reverse (-)
63273610 .. 63279673
6064 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG427000.1

Sequence Viewer

Length: 3741 bp
ATGAATTATGACCCGAAATATAATTGTGATCGTGTTATTGACAGAAAAGAAGCAGAGCTAATTCCAGATGAGGAAAAAAAGAAAGAAGCAGAGTTAATTGAAAGATTGGTAAGAAGGGTTATGACTGAAATAAACAAAACTCCGATGGGTCTCACTCCGTACCCGGTGGGACTTCATTCCCGAGTTGAAGAGGTGATGAAGCTGTTAGATATCGGATCCAAAACCGTTCAAGTTGTCGGAGTTCATGGCCTGGGCGGTATTGGCAAAACAACCCTTGCCAAGGCGCTTTTTAATAGACTTGTTGGTTATTTTGAGCGCCACAGTTTCATCTCAAATGTTAGAGAAATCTCAGCAGGACATGATGAGAATGGTTTAGTAACTCTTCAAAACATACTGGTTAGTGATCTTTCCAATAGGAAGATGGATAAAATTAATCAGACTACCGCTGGTATCGCTGCCATCAAAGTGGTTGTGAATGAGAAGCGAGTACTTGTTGTTCTGGATGATGTTGATAGCATAGACCAACTAAGTGCTTTAGTTGGCAACGGACAATGGTTTTATGAAGGAAGCCGAATTATTGTTACGACGAGAGATAGAGAACTCTTAACGAATCATCGTGTCAATCATAAATTGTATGAGGTCAGGGAGTTGGACGGGTCCGATGCTCTAGAACTTTTCAGTCACCATGCACTGGGAATTAGAGATATACCGGCAGATACCTTTCTGAAACTATCTAAGGAGATTGTGGATCTTACTGGTGGGCTACCGTTAGCGCTTGAAGTATTTGGCTCTAGCCTCTATGATAAGAGGACAGAAAAGGTATGGATAGATGCTCTTCATAAACTGAGACAGATTCGTCCACGGGATCTTCAGGATGTCCTGATGATAAGCTACAATGCTCTAGATGACCAGGAGAAGTGCATCTTTCTAGACATTGCATGTTTATTTGTTAAAATGAAGACCAAAAGAGAAGACGCAATTGTCATATTGAAGGGTTGTGGTTTTGATGGTGAGATAGTACTTACTGTCCTCACAGCAAGATCACTCATTAAGATCACTGAAGACACTACATTATGGATGCATGATCAAGTCAGAGACATGGGAAGACAAATCGTTACACAAGAAAACATTTCACATCCTGGCATGCGTAGTAGACTGTGGGATCGCGATGAAATCATGAATGTTTTCAAGTATGATAAGGGAACACCATCTATACAAGGGATTGTCCTAGACTTTGAAATGAAAAGGATGGTGAGTGATATAGGAGGTGACAGAATTTCTTGGGACAACTTTCGGAGAAGCCCCAATTGTACCACTGCGTTGACATACCTAAAGGAAAGATACAAAGCACATCGTAAAAGCCAAGAAGAGAGAAAGGGAGAGGTTAACATTTCCTCTACAGCTTTTGGAGCAATGGTCAATCTTAGGCTGCTTCAAATGAATAATGTGAATTTAGAAGGGAACTCAAAGTTTCTTCCTGCTGGAGTCAAGTGGTTACAATGGAAAGGTTGTCCTTTGAGATCTCTCCCTTCTGATTTCTTGCCTCCGCAACTCGCTGTCCTTGATCTTTCAGACAGCAATATCACAAGTTTGTGGGGAGGGTGCAGTATCATGGTATGCTTCAATTGTCTGACCTGCAGTGGAGACGGAAACAAGGTGGCTGAGAAATTGATGTTCTTGAATCTAAGCAACTGCATTTACCTAACTGCTATTCCTGATTTATCTGGGAATCGGGCGCTACAACAGCTTAATCTTGAGCATTGCATTTCACTGATTAAGCTTCATGGCTCGATTGGGAACCTGAACACATTAGTCCACTTGAACCTTCGAGAGTGTTCAAAGCTTGTTGAACTTCCAAGTGATGTCTCCGGCCTGAAAAAGCTCGAGTACCTAATCCTATCTGGTTGCTCACAGTTCAAAAAACTGCCAAACATGGACAGCTTGGTGTCACTGAAAGAACTTCTCCTTGATGACACTGCTATACAGAGTCTTCCCCAATCTATCTTCCGGCTTACAAAACTCGAAAAGCTCATCTTAAACCGGTGCAGTGAATTAAAAGAGCTACCTGTGGAGATTGGAAAGCTGTCTTCTTTGAAAGAAATCTCGCTTAATGGATCTGGTTTGGAAAAACTACCTAATTCCATTGGCTCTTTGGCAAACCTTGAGAAATTAAGTTTGTTCTGGTGTAAATCACTGACCACTCTTCCCAATTCCATTGGGAAGCTCAATTTCTTAGTGGAAGTTAGTACTTATGGTACTCCACTCACAGAACTGCCTGTGACCATTGGATCGTTGTCAAGCTTGAAGGACTTATCAGTTGGGCACGGTCAATTTCTGAGCTCATTGCCTGATTCAGTTGGAAGACTGCGCTCTTTAGTTGTGCTAAAGATAGAGCAGACATCAATCACAGACCTCCCACAGGAGATTGGTGAATTGAAAACACTTGAGAAACTTGAGCTGAGGAAATGTGAATCTCTTAGATCATTACCAGAGTCAATTGGAAGCATGAGGGCACTAACTTCTATAATCATAACTGCAGCAAATATCACCGAGTTGCCTGAATCCATAGGGAGGCTGGAGAATCTTACCATATTACAGCTGAACACATGTAGACAGTTCCGCAAGCTTCCAGCATCAATAGAACACTTAAAATCCTTGCACCGATTGCAGATGGTGAAAACTGCAGTGACAGAACTACCTGAAACCTTCGGAATGCTTTCTAGTTTGATGGTTTTGAGCATGGGAAAGAAGCCTCAAAATGGGAGACATGCAGAAGAGAAGTTTATACTTCCAGCTTCCTTCTCAAATCTGTCCCTGCTGTACGAACTGGATGCCTGTGCTTGTAATATATCTGGTGAAATTGCTGATGATTTCGAGAAGTTGTCATCGCTAGAAACTTTGAATCTGGGCCATAATAGTTTCTGCCGCCTTCCAGCCAGCCTGAGTGGTCTGTCCACTCTCGGAAAACTTTCATTGCCTCACTGTAAGAAGCTCAAATCTTTGCCACCGCTACCCTCAAGTTTGAAAGAGGTGGATATTGCAAACTGTACTGCATTAGAAAGTATATCTGATATATCAAACCTAGAGAACTTGACAGATTTCAACCTTACAAGCTGTGAGAAAGTGATGGATATTCCCGGCCTTGAATGCTTGGATTCCTTGGTAAGGTTGTATATGAGTGGCTGCAATGCTTGCACATCAGCTGTTAAGAGAAGACTTGCGAAAAAGAGCTACCTGAGGAAAATACGCAATCTTAGCATGCCTGGAAGCAAGATACCCGACTGGTTTTCTCAAGAAATGGTTACATTCTCAAAACGAGAAAACCGCCCCCTCAAAAGTGTAATTCTCTGTGTTGTAGTCTCGCTCAACCATCAAACACCAGACGACACAAGAGAAGAATTTCCTGCCGTGGTAGATATTCAAGCACAGATCCTCATACTGGATTCTCCTACTTTCACTACAACATTGGTCTTATCGGGAGTACCAAATACAAATGAAGATCAATTTCACTTGTGCCGGTATCCAACTGATCATCCTTTGGTATCCCAATTGAAAGATGGTTATAAAATACACGTAATGAGGAGAGAGCCGCCACATAGGAAAGGGGTGGAGCTGAAGAAGTGGGGGCTTTATTTGATTTACGAAGGTGATGATGACTATGAAGGAGATGAAGAATCATTAAATGAAAGCCAGCAATCCCTTTCAGAACAACTGGCAAATTTCTTCAGCTCTTTTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1246

Amino Acids

139.48

Weight (kDa)

6.41

Isoelectric Point (pI)

41.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 61 - 230 1.4e-19 NB-ARC domain
ATPase_2 PF01637 61 - 259 7.9e-07 ATPase domain predominantly from Archaea
NACHT PF05729 78 - 229 1.4e-06 NACHT domain
WHD_ROQ1 PF23282 303 - 373 5.1e-14 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 632 - 730 1.1e-11 Leucine-rich repeat region
LRR_14 PF23598 731 - 796 2e-07 Leucine-rich repeat region
LRR_14 PF23598 785 - 870 1.1e-12 Leucine-rich repeat region
LRR_13 PF23286 812 - 882 7.1e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 872 - 1099 4.4e-14 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3564
AasI GACNNNNNNGTC 2 cut(s) 855, 980
Acc36I ACCTGC 1 cut(s) 1643
AccB7I CCANNNNNTGG 1 cut(s) 691
AccI GTMKAC 2 cut(s) 1153, 2611
AccII CGCG 1 cut(s) 1167
AciI CCGC 8 cut(s) 255, 444, 1547, 2620, 2926, 3008, 3325, 3590
AclWI GGATC 8 cut(s) 210, 223, 756, 873, 1170, 2122, 2296, 3424
AcsI RAATTY 4 cut(s) 1275, 1450, 3398, 3718
AcuI CTGAAG 4 cut(s) 854, 1080, 3635, 3709
AfeI AGCGCT 1 cut(s) 774
AfiI CCNNNNNNNGG 7 cut(s) 280, 691, 2419, 2571, 2759, 3165, 3439
AflIII ACRYGT 2 cut(s) 2606, 3571
AgeI ACCGGT 1 cut(s) 2040
AjnI CCWGG 4 cut(s) 249, 909, 1138, 3262
Alw21I GWGCWC 1 cut(s) 2342
Alw26I GTCTC 7 cut(s) 155, 841, 1089, 1638, 1870, 2758, 3364
AlwI GGATC 8 cut(s) 210, 223, 756, 873, 1170, 2122, 2296, 3424
Ama87I CYCGRG 2 cut(s) 180, 1883
Aor51HI AGCGCT 1 cut(s) 774
AoxI GGCC 4 cut(s) 247, 1870, 2908, 3139
ApeKI GCWGC 4 cut(s) 455, 1429, 2537, 3183
ApoI RAATTY 4 cut(s) 1275, 1450, 3398, 3718
AseI ATTAAT 1 cut(s) 432
AsiGI ACCGGT 1 cut(s) 2040
Asp700I GAANNNNTTC 4 cut(s) 1184, 2716, 2968, 3399
AspLEI GCGC 5 cut(s) 286, 318, 775, 1738, 2370
AspS9I GGNCC 2 cut(s) 657, 2908
AsuC2I CCSGG 2 cut(s) 164, 3138
AvaI CYCGRG 2 cut(s) 180, 1883
AvaII GGWCC 1 cut(s) 657
AxyI CCTNAGG 1 cut(s) 3236
BaeGI GKGCMC 2 cut(s) 2325, 2515
BamHI GGATCC 1 cut(s) 215
BanII GRGCYC 1 cut(s) 2342
BbsI GAAGAC 8 cut(s) 965, 978, 1068, 1111, 1982, 2079, 2368, 3220
Bbv12I GWGCWC 1 cut(s) 2342
BbvCI CCTCAGC 1 cut(s) 2459
BbvI GCAGC 4 cut(s) 442, 1416, 2549, 3170
BceAI ACGGC 1 cut(s) 3392
BcgI CGANNNNNNTGC 2 cut(s) 2813, 2847
BciT130I CCWGG 4 cut(s) 251, 911, 1140, 3264
BciVI GTATCC 2 cut(s) 3531, 3553
BclI TGATCA 2 cut(s) 1084, 3529
BcnI CCSGG 2 cut(s) 164, 3138
BcoDI GTCTC 7 cut(s) 155, 841, 1089, 1638, 1870, 2758, 3364
BfaI CTAG 8 cut(s) 668, 792, 902, 929, 1229, 2721, 2891, 3083
BfmI CTRYAG 4 cut(s) 1398, 1636, 2535, 2682
BfoI RGCGCY 4 cut(s) 287, 319, 776, 1739
BfuAI ACCTGC 1 cut(s) 1643
BfuI GTATCC 2 cut(s) 3531, 3553
BglII AGATCT 1 cut(s) 1520
BisI GCNGC 6 cut(s) 456, 1430, 2538, 2926, 3184, 3590
BlsI GCNGC 6 cut(s) 457, 1431, 2539, 2927, 3185, 3591
BmcAI AGTACT 3 cut(s) 489, 1020, 2248
Bme1390I CCNGG 6 cut(s) 164, 251, 911, 1140, 3138, 3264
Bme18I GGWCC 1 cut(s) 657
BmeT110I CYCGRG 2 cut(s) 180, 1883
BmgT120I GGNCC 2 cut(s) 657, 2908
BmiI GGNNCC 3 cut(s) 217, 658, 1799
BmrFI CCNGG 6 cut(s) 164, 251, 911, 1140, 3138, 3264
BmrI ACTGGG 1 cut(s) 701
BmsI GCATC 6 cut(s) 652, 820, 930, 1068, 2642, 2821
BmuI ACTGGG 1 cut(s) 701
BpiI GAAGAC 8 cut(s) 965, 978, 1068, 1111, 1982, 2079, 2368, 3220
BpmI CTGGAG 2 cut(s) 1503, 2597
Bpu10I CCTNAGC 1 cut(s) 2459
BpuEI CTTGAG 6 cut(s) 1775, 2183, 2465, 2474, 3001, 3276
BpuMI CCSGG 2 cut(s) 164, 3138
BsaAI YACGTR 1 cut(s) 3574
BsaI GGTCTC 1 cut(s) 155
BsaJI CCNNGG 5 cut(s) 250, 279, 860, 3159, 3408
BsaWI WCCGGW 1 cut(s) 2040
BsaXI ACNNNNNCTCC 2 cut(s) 1401, 1431
Bsc4I CCNNNNNNNGG 7 cut(s) 280, 691, 2419, 2571, 2759, 3165, 3439
Bse118I RCCGGY 3 cut(s) 709, 2040, 3516
Bse1I ACTGG 7 cut(s) 399, 696, 760, 2832, 3287, 3444, 3717
Bse21I CCTNAGG 1 cut(s) 3236
Bse3DI GCAATG 6 cut(s) 933, 1419, 1759, 2342, 2972, 3193
BseBI CCWGG 4 cut(s) 251, 911, 1140, 3264
BseDI CCNNGG 5 cut(s) 250, 279, 860, 3159, 3408
BseGI GGATG 7 cut(s) 508, 880, 1083, 1135, 1254, 2836, 3532
BseLI CCNNNNNNNGG 7 cut(s) 280, 691, 2419, 2571, 2759, 3165, 3439
BseMI GCAATG 6 cut(s) 933, 1419, 1759, 2342, 2972, 3193
BseMII CTCAG 7 cut(s) 363, 836, 1653, 2327, 2450, 2933, 3227
BseNI ACTGG 7 cut(s) 399, 696, 760, 2832, 3287, 3444, 3717
BseRI GAGGAG 1 cut(s) 3595
BseSI GKGCMC 2 cut(s) 2325, 2515
BseXI GCAGC 4 cut(s) 442, 1416, 2549, 3170
BsgI GTGCAG 2 cut(s) 1624, 2065
Bsh1236I CGCG 1 cut(s) 1167
BshFI GGCC 4 cut(s) 249, 1872, 2910, 3141
BshTI ACCGGT 1 cut(s) 2040
BsiHKAI GWGCWC 1 cut(s) 2342
BsiHKCI CYCGRG 2 cut(s) 180, 1883
BsiSI CCGG 7 cut(s) 164, 710, 1869, 2008, 2041, 3138, 3517
BslFI GGGAC 3 cut(s) 183, 1298, 2797
BslI CCNNNNNNNGG 7 cut(s) 280, 691, 2419, 2571, 2759, 3165, 3439
BsmAI GTCTC 7 cut(s) 155, 841, 1089, 1638, 1870, 2758, 3364
BsmBI CGTCTC 1 cut(s) 1638
BsmFI GGGAC 3 cut(s) 183, 1298, 2797
BsmI GAATGC 2 cut(s) 2718, 3152
BsnI GGCC 4 cut(s) 249, 1872, 2910, 3141
Bso31I GGTCTC 1 cut(s) 155
BsoBI CYCGRG 2 cut(s) 180, 1883
Bsp1286I GDGCHC 3 cut(s) 2325, 2342, 2515
Bsp68I TCGCGA 1 cut(s) 1167
BspACI CCGC 8 cut(s) 255, 444, 1547, 2620, 2926, 3008, 3325, 3590
BspANI GGCC 4 cut(s) 249, 1872, 2910, 3141
BspCNI CTCAG 7 cut(s) 362, 837, 1654, 2328, 2451, 2934, 3228
BspFNI CGCG 1 cut(s) 1167
BspHI TCATGA 1 cut(s) 1176
BspLI GGNNCC 3 cut(s) 217, 658, 1799
BspMAI CTGCAG 3 cut(s) 1640, 2539, 2686
BspMI ACCTGC 1 cut(s) 1643
BspPI GGATC 8 cut(s) 210, 223, 756, 873, 1170, 2122, 2296, 3424
BspQI GCTCTTC 1 cut(s) 840
BspTNI GGTCTC 1 cut(s) 155
BsrDI GCAATG 6 cut(s) 933, 1419, 1759, 2342, 2972, 3193
BsrFI RCCGGY 3 cut(s) 709, 2040, 3516
BsrI ACTGG 7 cut(s) 399, 696, 760, 2832, 3287, 3444, 3717
BssAI RCCGGY 3 cut(s) 709, 2040, 3516
BssECI CCNNGG 5 cut(s) 250, 279, 860, 3159, 3408
BssT1I CCWWGG 2 cut(s) 279, 3159
Bst2UI CCWGG 4 cut(s) 251, 911, 1140, 3264
Bst6I CTCTTC 6 cut(s) 183, 387, 840, 1362, 2208, 2769
BstAPI GCANNNNNTGC 2 cut(s) 2665, 3192
BstBAI YACGTR 1 cut(s) 3574
BstC8I GCNNGC 6 cut(s) 1145, 2624, 2938, 3193, 3260, 3692
BstDSI CCRYGG 2 cut(s) 860, 3408
BstENI CCTNNNNNAGG 3 cut(s) 278, 2417, 3163
BstF5I GGATG 7 cut(s) 508, 880, 1083, 1135, 1254, 2836, 3532
BstFNI CGCG 1 cut(s) 1167
BstH2I RGCGCY 4 cut(s) 287, 319, 776, 1739
BstHHI GCGC 5 cut(s) 286, 318, 775, 1738, 2370
BstMAI GTCTC 7 cut(s) 155, 841, 1089, 1638, 1870, 2758, 3364
BstMWI GCNNNNNNNGC 8 cut(s) 261, 452, 1409, 1744, 2665, 3147, 3192, 3255
BstNI CCWGG 4 cut(s) 251, 911, 1140, 3264
BstNSI RCATGY 5 cut(s) 942, 1147, 2610, 2771, 3262
BstSCI CCNGG 6 cut(s) 162, 249, 909, 1138, 3136, 3262
BstSFI CTRYAG 4 cut(s) 1398, 1636, 2535, 2682
BstSLI GKGCMC 2 cut(s) 2325, 2515
BstUI CGCG 1 cut(s) 1167
BstV1I GCAGC 4 cut(s) 442, 1416, 2549, 3170
BstV2I GAAGAC 8 cut(s) 965, 978, 1068, 1111, 1982, 2079, 2368, 3220
BstX2I RGATCY 6 cut(s) 215, 748, 865, 1520, 2114, 3429
BstXI CCANNNNNNTGG 1 cut(s) 466
BstYI RGATCY 6 cut(s) 215, 748, 865, 1520, 2114, 3429
Bsu36I CCTNAGG 1 cut(s) 3236
BsuI GTATCC 2 cut(s) 3531, 3553
BsuRI GGCC 4 cut(s) 249, 1872, 2910, 3141
BtgI CCRYGG 2 cut(s) 860, 3408
BtgZI GCGATG 2 cut(s) 1182, 2871
BtsCI GGATG 7 cut(s) 508, 880, 1083, 1135, 1254, 2836, 3532
BtsI GCAGTG 5 cut(s) 1314, 1645, 1974, 2053, 2691
BtuMI TCGCGA 1 cut(s) 1167
BveI ACCTGC 1 cut(s) 1643
Cac8I GCNNGC 6 cut(s) 1145, 2624, 2938, 3193, 3260, 3692
CciI TCATGA 1 cut(s) 1176
CfoI GCGC 5 cut(s) 286, 318, 775, 1738, 2370
Cfr10I RCCGGY 3 cut(s) 709, 2040, 3516
Cfr13I GGNCC 2 cut(s) 657, 2908
CseI GACGC 1 cut(s) 983
CspAI ACCGGT 1 cut(s) 2040
DrdI GACNNNNNNGTC 2 cut(s) 855, 980
DseDI GACNNNNNNGTC 2 cut(s) 855, 980
Eam1104I CTCTTC 6 cut(s) 183, 387, 840, 1362, 2208, 2769
EarI CTCTTC 6 cut(s) 183, 387, 840, 1362, 2208, 2769
Ecl136II GAGCTC 1 cut(s) 2340
Eco130I CCWWGG 2 cut(s) 279, 3159
Eco24I GRGCYC 1 cut(s) 2342
Eco31I GGTCTC 1 cut(s) 155
Eco32I GATATC 1 cut(s) 211
Eco47I GGWCC 1 cut(s) 657
Eco47III AGCGCT 1 cut(s) 774
Eco53kI GAGCTC 1 cut(s) 2340
Eco57I CTGAAG 4 cut(s) 854, 1080, 3635, 3709
Eco81I CCTNAGG 1 cut(s) 3236
Eco88I CYCGRG 2 cut(s) 180, 1883
EcoICRI GAGCTC 1 cut(s) 2340
EcoNI CCTNNNNNAGG 3 cut(s) 278, 2417, 3163
EcoRII CCWGG 4 cut(s) 249, 909, 1138, 3262
EcoRV GATATC 1 cut(s) 211
EcoT14I CCWWGG 2 cut(s) 279, 3159
EcoT22I ATGCAT 1 cut(s) 1083
EcoT38I GRGCYC 1 cut(s) 2342
ErhI CCWWGG 2 cut(s) 279, 3159
Esp3I CGTCTC 1 cut(s) 1638
FalI AAGNNNNNCTT 4 cut(s) 908, 940, 2018, 2050
FaqI GGGAC 3 cut(s) 183, 1298, 2797
FbaI TGATCA 2 cut(s) 1084, 3529
FblI GTMKAC 2 cut(s) 1153, 2611
Fnu4HI GCNGC 6 cut(s) 456, 1430, 2538, 2926, 3184, 3590
FokI GGATG 7 cut(s) 515, 887, 1090, 1122, 1261, 2843, 3519
FriOI GRGCYC 1 cut(s) 2342
Fsp4HI GCNGC 6 cut(s) 456, 1430, 2538, 2926, 3184, 3590
FspBI CTAG 8 cut(s) 668, 792, 902, 929, 1229, 2721, 2891, 3083
GlaI GCGC 5 cut(s) 285, 317, 774, 1737, 2369
GluI GCNGC 6 cut(s) 456, 1430, 2538, 2926, 3184, 3590
GsuI CTGGAG 2 cut(s) 1503, 2597
HaeII RGCGCY 4 cut(s) 287, 319, 776, 1739
HaeIII GGCC 4 cut(s) 249, 1872, 2910, 3141
HapII CCGG 7 cut(s) 164, 710, 1869, 2008, 2041, 3138, 3517
HgaI GACGC 1 cut(s) 983
HhaI GCGC 5 cut(s) 286, 318, 775, 1738, 2370
Hin6I GCGC 5 cut(s) 284, 316, 773, 1736, 2368
HinP1I GCGC 5 cut(s) 284, 316, 773, 1736, 2368
HincII GTYRAC 2 cut(s) 1323, 1387
HindII GTYRAC 2 cut(s) 1323, 1387
HindIII AAGCTT 4 cut(s) 1778, 1841, 2299, 2624
HpaI GTTAAC 1 cut(s) 1387
HpaII CCGG 7 cut(s) 164, 710, 1869, 2008, 2041, 3138, 3517
Hpy166II GTNNAC 7 cut(s) 860, 1154, 1323, 1387, 1816, 2612, 2955
Hpy8I GTNNAC 7 cut(s) 860, 1154, 1323, 1387, 1816, 2612, 2955
Hpy99I CGWCG 1 cut(s) 589
HpyCH4IV ACGT 1 cut(s) 3573
HpyF10VI GCNNNNNNNGC 8 cut(s) 261, 452, 1409, 1744, 2665, 3147, 3192, 3255
HpySE526I ACGT 1 cut(s) 3573
HspAI GCGC 5 cut(s) 284, 316, 773, 1736, 2368
Ksp22I TGATCA 2 cut(s) 1084, 3529
KspAI GTTAAC 1 cut(s) 1387
LguI GCTCTTC 1 cut(s) 840
LmnI GCTCC 2 cut(s) 1409, 3610
Lsp1109I GCAGC 4 cut(s) 442, 1416, 2549, 3170
LweI GCATC 6 cut(s) 652, 820, 930, 1068, 2642, 2821
MaeI CTAG 8 cut(s) 668, 792, 902, 929, 1229, 2721, 2891, 3083
MaeII ACGT 1 cut(s) 3573
MfeI CAATTG 5 cut(s) 978, 1306, 1624, 2496, 3548
MflI RGATCY 6 cut(s) 215, 748, 865, 1520, 2114, 3429
MhlI GDGCHC 3 cut(s) 2325, 2342, 2515
MlyI GAGTC 3 cut(s) 1494, 1996, 2501
MmeI TCCRAC 4 cut(s) 217, 630, 2338, 3548
Mph1103I ATGCAT 1 cut(s) 1083
MroXI GAANNNNTTC 4 cut(s) 1184, 2716, 2968, 3399
MslI CAYNNNNRTG 1 cut(s) 464
MspA1I CMGCKG 3 cut(s) 446, 2599, 3203
MspI CCGG 7 cut(s) 164, 710, 1869, 2008, 2041, 3138, 3517
MspR9I CCNGG 6 cut(s) 164, 251, 911, 1140, 3138, 3264
MunI CAATTG 5 cut(s) 978, 1306, 1624, 2496, 3548
Mva1269I GAATGC 2 cut(s) 2718, 3152
MvaI CCWGG 4 cut(s) 251, 911, 1140, 3264
MvnI CGCG 1 cut(s) 1167
MwoI GCNNNNNNNGC 8 cut(s) 261, 452, 1409, 1744, 2665, 3147, 3192, 3255
NciI CCSGG 2 cut(s) 164, 3138
NlaIV GGNNCC 3 cut(s) 217, 658, 1799
NmuCI GTSAC 5 cut(s) 680, 1268, 1947, 2278, 2686
NruI TCGCGA 1 cut(s) 1167
NsiI ATGCAT 1 cut(s) 1083
NspI RCATGY 5 cut(s) 942, 1147, 2610, 2771, 3262
PaeI GCATGC 2 cut(s) 1147, 3262
PaeR7I CTCGAG 1 cut(s) 1883
PagI TCATGA 1 cut(s) 1176
PciI ACATGT 1 cut(s) 2606
PciSI GCTCTTC 1 cut(s) 840
PctI GAATGC 2 cut(s) 2718, 3152
PdmI GAANNNNTTC 4 cut(s) 1184, 2716, 2968, 3399
PflMI CCANNNNNTGG 1 cut(s) 691
PinAI ACCGGT 1 cut(s) 2040
PkrI GCNGC 6 cut(s) 457, 1431, 2539, 2927, 3185, 3591
PleI GAGTC 3 cut(s) 1493, 1995, 2500
PpsI GAGTC 3 cut(s) 1493, 1995, 2500
Ppu21I YACGTR 1 cut(s) 3574
PscI ACATGT 1 cut(s) 2606
PshBI ATTAAT 1 cut(s) 432
PsiI TTATAA 1 cut(s) 3564
Psp124BI GAGCTC 1 cut(s) 2342
Psp6I CCWGG 4 cut(s) 249, 909, 1138, 3262
PspGI CCWGG 4 cut(s) 249, 909, 1138, 3262
PspN4I GGNNCC 3 cut(s) 217, 658, 1799
PspPI GGNCC 2 cut(s) 657, 2908
PspXI VCTCGAGB 1 cut(s) 1883
PsrI GAACNNNNNNTAC 2 cut(s) 480, 512
PstI CTGCAG 3 cut(s) 1640, 2539, 2686
PsuI RGATCY 6 cut(s) 215, 748, 865, 1520, 2114, 3429
PvuII CAGCTG 2 cut(s) 2599, 3203
RruI TCGCGA 1 cut(s) 1167
RseI CAYNNNNRTG 1 cut(s) 464
SacI GAGCTC 1 cut(s) 2342
SapI GCTCTTC 1 cut(s) 840
SatI GCNGC 6 cut(s) 456, 1430, 2538, 2926, 3184, 3590
Sau96I GGNCC 2 cut(s) 657, 2908
ScaI AGTACT 3 cut(s) 489, 1020, 2248
SchI GAGTC 3 cut(s) 1494, 1996, 2501
ScrFI CCNGG 6 cut(s) 164, 251, 911, 1140, 3138, 3264
SduI GDGCHC 3 cut(s) 2325, 2342, 2515
SfaNI GCATC 6 cut(s) 652, 820, 930, 1068, 2642, 2821
SfcI CTRYAG 4 cut(s) 1398, 1636, 2535, 2682
Sfr274I CTCGAG 1 cut(s) 1883
SinI GGWCC 1 cut(s) 657
SlaI CTCGAG 1 cut(s) 1883
SmiMI CAYNNNNRTG 1 cut(s) 464
SmlI CTYRAG 7 cut(s) 1754, 1883, 2162, 2444, 2453, 3016, 3291
SmoI CTYRAG 7 cut(s) 1754, 1883, 2162, 2444, 2453, 3016, 3291
SphI GCATGC 2 cut(s) 1147, 3262
SsiI CCGC 8 cut(s) 255, 444, 1547, 2620, 2926, 3008, 3325, 3590
SspMI CTAG 8 cut(s) 668, 792, 902, 929, 1229, 2721, 2891, 3083
SstI GAGCTC 1 cut(s) 2342
StyD4I CCNGG 6 cut(s) 162, 249, 909, 1138, 3136, 3262
StyI CCWWGG 2 cut(s) 279, 3159
TaiI ACGT 1 cut(s) 3576
TaqI TCGA 5 cut(s) 1790, 1828, 1884, 2022, 2874
TatI WGTACW 4 cut(s) 487, 1018, 2246, 3047
TauI GCSGC 2 cut(s) 2928, 3592
TseFI GTSAC 5 cut(s) 680, 1268, 1947, 2278, 2686
TseI GCWGC 4 cut(s) 455, 1429, 2537, 3183
Tsp45I GTSAC 5 cut(s) 680, 1268, 1947, 2278, 2686
TspGWI ACGGA 3 cut(s) 147, 561, 1662
Van91I CCANNNNNTGG 1 cut(s) 691
VpaK11BI GGWCC 1 cut(s) 657
VspI ATTAAT 1 cut(s) 432
XagI CCTNNNNNAGG 3 cut(s) 278, 2417, 3163
XapI RAATTY 4 cut(s) 1275, 1450, 3398, 3718
XbaI TCTAGA 3 cut(s) 667, 901, 928
XceI RCATGY 5 cut(s) 942, 1147, 2610, 2771, 3262
XcmI CCANNNNNNNNNTGG 4 cut(s) 418, 2149, 2572, 3554
XhoI CTCGAG 1 cut(s) 1883
XmiI GTMKAC 2 cut(s) 1153, 2611
XmnI GAANNNNTTC 4 cut(s) 1184, 2716, 2968, 3399
XspI CTAG 8 cut(s) 668, 792, 902, 929, 1229, 2721, 2891, 3083
ZrmI AGTACT 3 cut(s) 489, 1020, 2248
Zsp2I ATGCAT 1 cut(s) 1083
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.