Rmu_sc0003784.1_g000008

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003784.1
OG0001217
Physical Location & Seq
Reverse (-)
36832 .. 39652
2821 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003784.1_g000008.1.cds

Sequence Viewer

Length: 2202 bp
atgccgtccgacggtgccgtgggcggtttccggctccggtgggacgtgttcctaagctttagaggagaagacacccgccacaccatcaccaacagcctctacaacgcgctcgaacgacgcggcgtacgagtcttccgcgacgacgacggcctcaaccgcggcgacgacatcgcgccgagtcttctggaagcgatcgaggactcggcggcggccgtcgtcgtgttgtcgccgagatacgccgactcgaggtggtgcctcgaggagctcgcgaagatctgcgacggcggccggagactgatcctgccagtgttttaccaagtggacccctccgacgtccggcgacagagcggaccgtttgagaaggacttcagatctcatgaagagcgttttgaaagcgacaaggtctctaaatggagaaacgccatggccaaagtaggtggcaaagctggttttgttgtcaacagcagagttgattacactattcctaactccctcggtaaccttaataacttaacggaatttcttacttacggtactccaattgaagaactgccgttgagttttggttcattatcaaacttgaaggaattatctgtgggacacggccaatttctgcaaacactaccagattccattggacgactaaaatcattagttgtgttaaagatagatgagacatcaatcacaggtcttccacaagagattagtgccttgaaaacacttgagaaacttgagctgaggaaatgtgaatctcttagatcattaccagagtctattggaagcatgaagtcactaacttctataatcataactgcagcaaatatcaccgagttgcctgaatccatagggaggctggaaaatcttaccatgttacagttgaacagatgtaaacagttctgcaagcttccagcatcaataggacagttaaaatccttgcatcgattgcagatggtggaaactgcagtgacagaactacctgaaagcttcggaatgctttctagcttgatggttttgagaatgggaaagaagcctcaaaatgggagacatacagaagagaagtttatacttccagcttccttctcaaatctgtccctgctgtctgaactggatgctcgtgcttgtaatatatctggtgaaattcctgatgatttcgagaagttatcatcacttgaaactttgaatctcagccataatagtttctgccgccttccagccagcctgagtggtctgtccactctccgagaactttcattgcctcactgtaagaagctcaagtctttgcctccacttccctcaagtttggaaaaggtagatgttgcaaactgtgttgcattagaaagtatatctgatgtttcaaacctggagaacttgacagatttcaatcttacaaattgtgggaaagtgttagatattcccggcattgaatgcttggattccttgacgaggctgtttatgagtggctgcaacagttgctcatcagctgtcaagaaaagactagcaaaaaagagttacctgaggacaatacggactcttagcattcctggaagcaagatacctgactggttttctcaagatgtgattacattctcagaacggaaaaaccatgttctcaaagggttgattatagctgttgtagtttctctcaaccatcaaataccggatgacctaagagaagaactcccagctgtggtagatattgtagcaaagatcctcatactggattctcctaccttcacttcaacgttggtcttatcaggagtaccaaatacaaatgaagatcaatttttcttgtgccggtatccaatttctcatcctttggttttccaactgaaggatggttataagatacatgtagagaggagagagccgccgtatgtgaaaggagtggagctgaagaagtgggggattcatttggtgtatgaaggtgatgatgattacgaggaagacgaagagtcattgaaggaaagccagcagtccctttcagaaaaactggccaagttctacggctcttttgatgaagaagccgactcttcctccactcatgagcaaaatgatttcacctctgaatctagtcataatgaagatgaaggaatgacacaggagatgagagaaagtgaccgccgttacttcaattctttcttcctagtgctttctcttgtctttctgttgttgagttggttctggttctggttctgggtctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

733

Amino Acids

82.39

Weight (kDa)

5.39

Isoelectric Point (pI)

48.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1842
AatII GACGTC 1 cut(s) 336
AbsI CCTCGAGG 1 cut(s) 257
AccB1I GGYRCC 2 cut(s) 14, 252
AccBSI CCGCTC 1 cut(s) 348
AccII CGCG 6 cut(s) 107, 120, 138, 159, 173, 269
AclI AACGTT 1 cut(s) 1742
AclWI GGATC 2 cut(s) 292, 1702
AcoI YGGCCR 5 cut(s) 210, 286, 426, 604, 1992
AcsI RAATTY 2 cut(s) 518, 1137
AcuI CTGAAG 3 cut(s) 352, 1850, 1913
AcyI GRCGYC 1 cut(s) 333
AfaI GTAC 3 cut(s) 126, 535, 1761
AfiI CCNNNNNNNGG 9 cut(s) 11, 246, 336, 849, 1037, 1443, 1687, 1717, 1831
AflIII ACRYGT 2 cut(s) 45, 1849
AhdI GACNNNNNGTC 1 cut(s) 1951
AjiI CACGTC 1 cut(s) 46
AjnI CCWGG 2 cut(s) 1359, 1540
Alw21I GWGCWC 1 cut(s) 267
Alw26I GTCTC 4 cut(s) 286, 409, 668, 1036
AlwI GGATC 2 cut(s) 292, 1702
Ama87I CYCGRG 2 cut(s) 244, 257
AoxI GGCC 6 cut(s) 148, 210, 286, 426, 604, 1992
ApeKI GCWGC 2 cut(s) 815, 1461
ApoI RAATTY 2 cut(s) 518, 1137
Asp700I GAANNNNTTC 2 cut(s) 365, 994
AspLEI GCGC 2 cut(s) 109, 175
AspS9I GGNCC 2 cut(s) 322, 350
AsuC2I CCSGG 1 cut(s) 1416
AsuHPI GGTGA 5 cut(s) 79, 817, 1145, 1937, 2050
AvaI CYCGRG 2 cut(s) 244, 257
AvaII GGWCC 2 cut(s) 322, 350
AxyI CCTNAGG 1 cut(s) 1514
BalI TGGCCA 2 cut(s) 428, 1994
BanI GGYRCC 2 cut(s) 14, 252
BanII GRGCYC 1 cut(s) 267
BauI CACGAG 1 cut(s) 1113
BbsI GAAGAC 5 cut(s) 75, 124, 173, 683, 1950
Bbv12I GWGCWC 1 cut(s) 267
BbvCI CCTCAGC 1 cut(s) 737
BbvI GCAGC 2 cut(s) 827, 1448
BccI CCATC 5 cut(s) 92, 943, 1000, 1656, 1829
BceAI ACGGC 9 cut(s) 2, 163, 197, 298, 538, 619, 1855, 2020, 2106
BciT130I CCWGG 2 cut(s) 1361, 1542
BciVI GTATCC 1 cut(s) 1809
BcnI CCSGG 1 cut(s) 1416
BcoDI GTCTC 4 cut(s) 286, 409, 668, 1036
BfaI CTAG 4 cut(s) 999, 1496, 2070, 2144
BfmI CTRYAG 2 cut(s) 813, 960
BfuI GTATCC 1 cut(s) 1809
BglII AGATCT 2 cut(s) 273, 371
BisI GCNGC 9 cut(s) 121, 160, 207, 210, 286, 816, 1204, 1462, 1868
BlsI GCNGC 9 cut(s) 122, 161, 208, 211, 287, 817, 1205, 1463, 1869
Bme1390I CCNGG 3 cut(s) 1361, 1416, 1542
Bme18I GGWCC 2 cut(s) 322, 350
BmeRI GACNNNNNGTC 1 cut(s) 1951
BmeT110I CYCGRG 2 cut(s) 244, 257
BmgBI CACGTC 1 cut(s) 46
BmgT120I GGNCC 2 cut(s) 322, 350
BmiI GGNNCC 4 cut(s) 16, 35, 254, 324
BmrFI CCNGG 3 cut(s) 1361, 1416, 1542
BmsI GCATC 3 cut(s) 920, 946, 1099
BpiI GAAGAC 5 cut(s) 75, 124, 173, 683, 1950
BplI GAGNNNNNCTC 2 cut(s) 1662, 1694
BpmI CTGGAG 1 cut(s) 1382
Bpu10I CCTNAGC 2 cut(s) 53, 737
BpuEI CTTGAG 5 cut(s) 743, 752, 1256, 1279, 1554
BpuMI CCSGG 1 cut(s) 1416
Bsa29I ATCGAT 1 cut(s) 940
BsaBI GATNNNNATC 1 cut(s) 1380
BsaHI GRCGYC 1 cut(s) 333
BsaI GGTCTC 1 cut(s) 409
BsaJI CCNNGG 4 cut(s) 18, 157, 423, 493
BsaWI WCCGGW 2 cut(s) 36, 1657
BsaXI ACNNNNNCTCC 2 cut(s) 2018, 2048
Bsc4I CCNNNNNNNGG 9 cut(s) 11, 246, 336, 849, 1037, 1443, 1687, 1717, 1831
Bse118I RCCGGY 1 cut(s) 1794
Bse1I ACTGG 5 cut(s) 305, 1110, 1565, 1722, 1995
Bse21I CCTNAGG 1 cut(s) 1514
Bse3DI GCAATG 1 cut(s) 1250
Bse8I GATNNNNATC 1 cut(s) 1380
BseBI CCWGG 2 cut(s) 1361, 1542
BseCI ATCGAT 1 cut(s) 940
BseDI CCNNGG 4 cut(s) 18, 157, 423, 493
BseGI GGATG 4 cut(s) 1114, 1666, 1810, 1840
BseJI GATNNNNATC 1 cut(s) 1380
BseLI CCNNNNNNNGG 9 cut(s) 11, 246, 336, 849, 1037, 1443, 1687, 1717, 1831
BseMI GCAATG 1 cut(s) 1250
BseMII CTCAG 5 cut(s) 728, 1198, 1211, 1505, 1602
BseNI ACTGG 5 cut(s) 305, 1110, 1565, 1722, 1995
BseRI GAGGAG 3 cut(s) 78, 275, 1873
BseX3I CGGCCG 2 cut(s) 210, 286
BseXI GCAGC 2 cut(s) 827, 1448
BseYI CCCAGC 1 cut(s) 1681
Bsh1236I CGCG 6 cut(s) 107, 120, 138, 159, 173, 269
Bsh1285I CGRYCG 3 cut(s) 195, 213, 289
BshFI GGCC 6 cut(s) 150, 212, 288, 428, 606, 1994
BshNI GGYRCC 2 cut(s) 14, 252
BshVI ATCGAT 1 cut(s) 940
BsiEI CGRYCG 3 cut(s) 195, 213, 289
BsiHKAI GWGCWC 1 cut(s) 267
BsiHKCI CYCGRG 2 cut(s) 244, 257
BsiSI CCGG 7 cut(s) 31, 37, 289, 337, 1416, 1658, 1795
BsiWI CGTACG 1 cut(s) 124
BslFI GGGAC 4 cut(s) 56, 612, 1075, 1960
BslI CCNNNNNNNGG 9 cut(s) 11, 246, 336, 849, 1037, 1443, 1687, 1717, 1831
BsmAI GTCTC 4 cut(s) 286, 409, 668, 1036
BsmFI GGGAC 4 cut(s) 56, 612, 1075, 1960
BsmI GAATGC 3 cut(s) 996, 1430, 1536
BsnI GGCC 6 cut(s) 150, 212, 288, 428, 606, 1994
Bso31I GGTCTC 1 cut(s) 409
BsoBI CYCGRG 2 cut(s) 244, 257
Bsp1286I GDGCHC 1 cut(s) 267
Bsp143I GATC 7 cut(s) 192, 273, 297, 371, 758, 1707, 1777
Bsp19I CCATGG 1 cut(s) 423
Bsp68I TCGCGA 1 cut(s) 269
BspANI GGCC 6 cut(s) 150, 212, 288, 428, 606, 1994
BspCNI CTCAG 5 cut(s) 729, 1197, 1212, 1506, 1601
BspDI ATCGAT 1 cut(s) 940
BspFNI CGCG 6 cut(s) 107, 120, 138, 159, 173, 269
BspHI TCATGA 2 cut(s) 376, 2041
BspLI GGNNCC 4 cut(s) 16, 35, 254, 324
BspMAI CTGCAG 2 cut(s) 817, 964
BspPI GGATC 2 cut(s) 292, 1702
BspQI GCTCTTC 1 cut(s) 375
BspT107I GGYRCC 2 cut(s) 14, 252
BspTNI GGTCTC 1 cut(s) 409
BsrBI CCGCTC 1 cut(s) 348
BsrDI GCAATG 1 cut(s) 1250
BsrFI RCCGGY 1 cut(s) 1794
BsrI ACTGG 5 cut(s) 305, 1110, 1565, 1722, 1995
BssAI RCCGGY 1 cut(s) 1794
BssECI CCNNGG 4 cut(s) 18, 157, 423, 493
BssMI GATC 7 cut(s) 192, 273, 297, 371, 758, 1707, 1777
BssNI GRCGYC 1 cut(s) 333
BssSI CACGAG 1 cut(s) 1113
BssT1I CCWWGG 1 cut(s) 423
Bst2BI CACGAG 1 cut(s) 1113
Bst2UI CCWGG 2 cut(s) 1361, 1542
Bst4CI ACNGT 9 cut(s) 14, 354, 533, 876, 894, 924, 1262, 1325, 1469
Bst6I CTCTTC 4 cut(s) 375, 1047, 1944, 2035
BstACI GRCGYC 1 cut(s) 333
BstAPI GCANNNNNTGC 3 cut(s) 943, 1425, 1470
BstC8I GCNNGC 4 cut(s) 267, 902, 1216, 1970
BstDEI CTNAG 9 cut(s) 53, 737, 755, 1184, 1220, 1514, 1532, 1588, 1667
BstDSI CCRYGG 3 cut(s) 18, 157, 423
BstEII GGTNACC 1 cut(s) 497
BstENI CCTNNNNNAGG 1 cut(s) 1441
BstF5I GGATG 4 cut(s) 1114, 1666, 1810, 1840
BstFNI CGCG 6 cut(s) 107, 120, 138, 159, 173, 269
BstHHI GCGC 2 cut(s) 109, 175
BstKTI GATC 7 cut(s) 195, 276, 300, 374, 761, 1710, 1780
BstMAI GTCTC 4 cut(s) 286, 409, 668, 1036
BstMBI GATC 7 cut(s) 192, 273, 297, 371, 758, 1707, 1777
BstMCI CGRYCG 3 cut(s) 195, 213, 289
BstMWI GCNNNNNNNGC 5 cut(s) 156, 285, 943, 1425, 1470
BstNI CCWGG 2 cut(s) 1361, 1542
BstNSI RCATGY 1 cut(s) 1853
BstPI GGTNACC 1 cut(s) 497
BstSCI CCNGG 3 cut(s) 1359, 1414, 1540
BstSFI CTRYAG 2 cut(s) 813, 960
BstUI CGCG 6 cut(s) 107, 120, 138, 159, 173, 269
BstV1I GCAGC 2 cut(s) 827, 1448
BstV2I GAAGAC 5 cut(s) 75, 124, 173, 683, 1950
BstX2I RGATCY 3 cut(s) 273, 371, 1707
BstYI RGATCY 3 cut(s) 273, 371, 1707
BstZI CGGCCG 2 cut(s) 210, 286
Bsu15I ATCGAT 1 cut(s) 940
Bsu36I CCTNAGG 1 cut(s) 1514
BsuI GTATCC 1 cut(s) 1809
BsuRI GGCC 6 cut(s) 150, 212, 288, 428, 606, 1994
BsuTUI ATCGAT 1 cut(s) 940
BtgI CCRYGG 3 cut(s) 18, 157, 423
BtgZI GCGATG 1 cut(s) 154
BtrI CACGTC 1 cut(s) 46
BtsCI GGATG 4 cut(s) 1114, 1666, 1810, 1840
BtsI GCAGTG 1 cut(s) 969
BtsIMutI CAGTG 3 cut(s) 312, 969, 1258
BtuMI TCGCGA 1 cut(s) 269
Cac8I GCNNGC 4 cut(s) 267, 902, 1216, 1970
CciI TCATGA 2 cut(s) 376, 2041
CfoI GCGC 2 cut(s) 109, 175
Cfr10I RCCGGY 1 cut(s) 1794
Cfr13I GGNCC 2 cut(s) 322, 350
Cfr42I CCGCGG 1 cut(s) 160
ClaI ATCGAT 1 cut(s) 940
CpoI CGGWCCG 1 cut(s) 350
CseI GACGC 1 cut(s) 126
Csp6I GTAC 3 cut(s) 125, 534, 1760
CspCI CAANNNNNGTGG 2 cut(s) 418, 453
CspI CGGWCCG 1 cut(s) 350
CviAII CATG 7 cut(s) 377, 424, 784, 868, 1604, 1850, 2042
CviQI GTAC 3 cut(s) 125, 534, 1760
DdeI CTNAG 9 cut(s) 53, 737, 755, 1184, 1220, 1514, 1532, 1588, 1667
DpnI GATC 7 cut(s) 194, 275, 299, 373, 760, 1709, 1779
DpnII GATC 7 cut(s) 192, 273, 297, 371, 758, 1707, 1777
DriI GACNNNNNGTC 1 cut(s) 1951
EaeI YGGCCR 5 cut(s) 210, 286, 426, 604, 1992
EagI CGGCCG 2 cut(s) 210, 286
Eam1104I CTCTTC 4 cut(s) 375, 1047, 1944, 2035
Eam1105I GACNNNNNGTC 1 cut(s) 1951
EarI CTCTTC 4 cut(s) 375, 1047, 1944, 2035
Ecl136II GAGCTC 1 cut(s) 265
EclXI CGGCCG 2 cut(s) 210, 286
Eco130I CCWWGG 1 cut(s) 423
Eco24I GRGCYC 1 cut(s) 267
Eco31I GGTCTC 1 cut(s) 409
Eco47I GGWCC 2 cut(s) 322, 350
Eco52I CGGCCG 2 cut(s) 210, 286
Eco53kI GAGCTC 1 cut(s) 265
Eco57I CTGAAG 3 cut(s) 352, 1850, 1913
Eco81I CCTNAGG 1 cut(s) 1514
Eco88I CYCGRG 2 cut(s) 244, 257
Eco91I GGTNACC 1 cut(s) 497
EcoICRI GAGCTC 1 cut(s) 265
EcoNI CCTNNNNNAGG 1 cut(s) 1441
EcoO65I GGTNACC 1 cut(s) 497
EcoRII CCWGG 2 cut(s) 1359, 1540
EcoT14I CCWWGG 1 cut(s) 423
EcoT38I GRGCYC 1 cut(s) 267
ErhI CCWWGG 1 cut(s) 423
FaeI CATG 7 cut(s) 380, 427, 787, 871, 1607, 1853, 2045
FaqI GGGAC 4 cut(s) 56, 612, 1075, 1960
FatI CATG 7 cut(s) 376, 423, 783, 867, 1603, 1849, 2041
FauI CCCGC 1 cut(s) 83
Fnu4HI GCNGC 9 cut(s) 121, 160, 207, 210, 286, 816, 1204, 1462, 1868
FokI GGATG 4 cut(s) 1121, 1673, 1797, 1847
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 9 cut(s) 121, 160, 207, 210, 286, 816, 1204, 1462, 1868
FspBI CTAG 4 cut(s) 999, 1496, 2070, 2144
GlaI GCGC 2 cut(s) 108, 174
GluI GCNGC 9 cut(s) 121, 160, 207, 210, 286, 816, 1204, 1462, 1868
GsaI CCCAGC 1 cut(s) 1685
GsuI CTGGAG 1 cut(s) 1382
HaeIII GGCC 6 cut(s) 150, 212, 288, 428, 606, 1994
HapII CCGG 7 cut(s) 31, 37, 289, 337, 1416, 1658, 1795
HgaI GACGC 1 cut(s) 126
HhaI GCGC 2 cut(s) 109, 175
Hin1I GRCGYC 1 cut(s) 333
Hin1II CATG 7 cut(s) 380, 427, 787, 871, 1607, 1853, 2045
Hin6I GCGC 2 cut(s) 107, 173
HinP1I GCGC 2 cut(s) 107, 173
HincII GTYRAC 1 cut(s) 460
HindII GTYRAC 1 cut(s) 460
HindIII AAGCTT 3 cut(s) 55, 902, 982
HpaII CCGG 7 cut(s) 31, 37, 289, 337, 1416, 1658, 1795
HphI GGTGA 5 cut(s) 79, 817, 1145, 1937, 2050
Hpy166II GTNNAC 4 cut(s) 322, 460, 890, 1233
Hpy188III TCNNGA 9 cut(s) 185, 268, 377, 1142, 1153, 1486, 1571, 1755, 2042
Hpy8I GTNNAC 4 cut(s) 322, 460, 890, 1233
HpyAV CCTTC 9 cut(s) 355, 577, 1087, 1217, 1741, 1825, 1916, 1954, 2081
HpyCH4III ACNGT 9 cut(s) 14, 354, 533, 876, 894, 924, 1262, 1325, 1469
HpyCH4IV ACGT 3 cut(s) 45, 333, 1742
HpyCH4V TGCA 9 cut(s) 616, 815, 900, 937, 946, 962, 1319, 1331, 1464
HpyF10VI GCNNNNNNNGC 5 cut(s) 156, 285, 943, 1425, 1470
HpyF3I CTNAG 9 cut(s) 53, 737, 755, 1184, 1220, 1514, 1532, 1588, 1667
HpySE526I ACGT 3 cut(s) 45, 333, 1742
Hsp92I GRCGYC 1 cut(s) 333
Hsp92II CATG 7 cut(s) 380, 427, 787, 871, 1607, 1853, 2045
HspAI GCGC 2 cut(s) 107, 173
KspI CCGCGG 1 cut(s) 160
Kzo9I GATC 7 cut(s) 192, 273, 297, 371, 758, 1707, 1777
LguI GCTCTTC 1 cut(s) 375
LmnI GCTCC 3 cut(s) 39, 262, 1888
Lsp1109I GCAGC 2 cut(s) 827, 1448
LweI GCATC 3 cut(s) 920, 946, 1099
MaeI CTAG 4 cut(s) 999, 1496, 2070, 2144
MaeII ACGT 3 cut(s) 45, 333, 1742
MaeIII GTNAC 7 cut(s) 497, 789, 870, 964, 1508, 2114, 2123
MalI GATC 7 cut(s) 194, 275, 299, 373, 760, 1709, 1779
MbiI CCGCTC 1 cut(s) 348
MboI GATC 7 cut(s) 192, 273, 297, 371, 758, 1707, 1777
MfeI CAATTG 1 cut(s) 540
MflI RGATCY 3 cut(s) 273, 371, 1707
MhlI GDGCHC 1 cut(s) 267
MlsI TGGCCA 2 cut(s) 428, 1994
MluCI AATT 9 cut(s) 518, 540, 587, 608, 1137, 1390, 1781, 1803, 2131
MluNI TGGCCA 2 cut(s) 428, 1994
MlyI GAGTC 8 cut(s) 138, 187, 194, 236, 779, 1522, 1961, 2021
MmeI TCCRAC 3 cut(s) 33, 354, 1849
Mox20I TGGCCA 2 cut(s) 428, 1994
MroXI GAANNNNTTC 2 cut(s) 365, 994
MscI TGGCCA 2 cut(s) 428, 1994
MseI TTAA 4 cut(s) 504, 512, 662, 926
Msp20I TGGCCA 2 cut(s) 428, 1994
MspA1I CMGCKG 3 cut(s) 159, 1481, 1685
MspI CCGG 7 cut(s) 31, 37, 289, 337, 1416, 1658, 1795
MspR9I CCNGG 3 cut(s) 1361, 1416, 1542
MunI CAATTG 1 cut(s) 540
Mva1269I GAATGC 3 cut(s) 996, 1430, 1536
MvaI CCWGG 2 cut(s) 1361, 1542
MvnI CGCG 6 cut(s) 107, 120, 138, 159, 173, 269
MwoI GCNNNNNNNGC 5 cut(s) 156, 285, 943, 1425, 1470
NciI CCSGG 1 cut(s) 1416
NcoI CCATGG 1 cut(s) 423
NdeII GATC 7 cut(s) 192, 273, 297, 371, 758, 1707, 1777
NlaIII CATG 7 cut(s) 380, 427, 787, 871, 1607, 1853, 2045
NlaIV GGNNCC 4 cut(s) 16, 35, 254, 324
NmeAIII GCCGAG 3 cut(s) 182, 201, 255
NmuCI GTSAC 3 cut(s) 789, 964, 2114
NruI TCGCGA 1 cut(s) 269
NspI RCATGY 1 cut(s) 1853
PaeR7I CTCGAG 2 cut(s) 244, 257
PagI TCATGA 2 cut(s) 376, 2041
PciI ACATGT 1 cut(s) 1849
PciSI GCTCTTC 1 cut(s) 375
PcsI WCGNNNNNNNCGW 2 cut(s) 124, 1944
PctI GAATGC 3 cut(s) 996, 1430, 1536
PdmI GAANNNNTTC 2 cut(s) 365, 994
PfeI GAWTC 8 cut(s) 629, 749, 839, 1180, 1433, 1721, 1906, 2066
Pfl23II CGTACG 1 cut(s) 124
PflFI GACNNNGTC 1 cut(s) 401
PfoI TCCNGGA 1 cut(s) 1540
PkrI GCNGC 9 cut(s) 122, 161, 208, 211, 287, 817, 1205, 1463, 1869
Ple19I CGATCG 1 cut(s) 195
PleI GAGTC 8 cut(s) 137, 186, 194, 236, 778, 1522, 1960, 2021
PpsI GAGTC 8 cut(s) 137, 186, 194, 236, 778, 1522, 1960, 2021
PscI ACATGT 1 cut(s) 1849
PsiI TTATAA 1 cut(s) 1842
Psp124BI GAGCTC 1 cut(s) 267
Psp1406I AACGTT 1 cut(s) 1742
Psp6I CCWGG 2 cut(s) 1359, 1540
PspEI GGTNACC 1 cut(s) 497
PspFI CCCAGC 1 cut(s) 1681
PspGI CCWGG 2 cut(s) 1359, 1540
PspLI CGTACG 1 cut(s) 124
PspN4I GGNNCC 4 cut(s) 16, 35, 254, 324
PspPI GGNCC 2 cut(s) 322, 350
PspXI VCTCGAGB 2 cut(s) 244, 257
PstI CTGCAG 2 cut(s) 817, 964
PsuI RGATCY 3 cut(s) 273, 371, 1707
PsyI GACNNNGTC 1 cut(s) 401
PvuI CGATCG 1 cut(s) 195
PvuII CAGCTG 2 cut(s) 1481, 1685
RruI TCGCGA 1 cut(s) 269
RsaI GTAC 3 cut(s) 126, 535, 1761
RsaNI GTAC 3 cut(s) 125, 534, 1760
Rsr2I CGGWCCG 1 cut(s) 350
RsrII CGGWCCG 1 cut(s) 350
SacI GAGCTC 1 cut(s) 267
SacII CCGCGG 1 cut(s) 160
SapI GCTCTTC 1 cut(s) 375
SaqAI TTAA 4 cut(s) 504, 512, 662, 926
SatI GCNGC 9 cut(s) 121, 160, 207, 210, 286, 816, 1204, 1462, 1868
Sau3AI GATC 7 cut(s) 192, 273, 297, 371, 758, 1707, 1777
Sau96I GGNCC 2 cut(s) 322, 350
SchI GAGTC 8 cut(s) 138, 187, 194, 236, 779, 1522, 1961, 2021
ScrFI CCNGG 3 cut(s) 1361, 1416, 1542
SduI GDGCHC 1 cut(s) 267
SfaNI GCATC 3 cut(s) 920, 946, 1099
SfcI CTRYAG 2 cut(s) 813, 960
Sfr274I CTCGAG 2 cut(s) 244, 257
Sfr303I CCGCGG 1 cut(s) 160
SgrBI CCGCGG 1 cut(s) 160
SinI GGWCC 2 cut(s) 322, 350
SlaI CTCGAG 2 cut(s) 244, 257
SmlI CTYRAG 7 cut(s) 244, 257, 722, 731, 1271, 1294, 1569
SmoI CTYRAG 7 cut(s) 244, 257, 722, 731, 1271, 1294, 1569
Sse9I AATT 9 cut(s) 518, 540, 587, 608, 1137, 1390, 1781, 1803, 2131
SspMI CTAG 4 cut(s) 999, 1496, 2070, 2144
SstI GAGCTC 1 cut(s) 267
StyD4I CCNGG 3 cut(s) 1359, 1414, 1540
StyI CCWWGG 1 cut(s) 423
TaaI ACNGT 9 cut(s) 14, 354, 533, 876, 894, 924, 1262, 1325, 1469
TaiI ACGT 3 cut(s) 48, 336, 1745
TaqI TCGA 6 cut(s) 111, 195, 245, 258, 940, 1152
TasI AATT 9 cut(s) 518, 540, 587, 608, 1137, 1390, 1781, 1803, 2131
TauI GCSGC 7 cut(s) 123, 162, 209, 212, 288, 1206, 1870
TfiI GAWTC 8 cut(s) 629, 749, 839, 1180, 1433, 1721, 1906, 2066
Tru1I TTAA 4 cut(s) 504, 512, 662, 926
Tru9I TTAA 4 cut(s) 504, 512, 662, 926
TscAI CASTG 3 cut(s) 312, 969, 1265
TseFI GTSAC 3 cut(s) 789, 964, 2114
TseI GCWGC 2 cut(s) 815, 1461
Tsp45I GTSAC 3 cut(s) 789, 964, 2114
TspGWI ACGGA 3 cut(s) 530, 1540, 1609
TspRI CASTG 3 cut(s) 312, 969, 1265
Tth111I GACNNNGTC 1 cut(s) 401
VpaK11BI GGWCC 2 cut(s) 322, 350
XagI CCTNNNNNAGG 1 cut(s) 1441
XapI RAATTY 2 cut(s) 518, 1137
XceI RCATGY 1 cut(s) 1853
XcmI CCANNNNNNNNNTGG 2 cut(s) 850, 1832
XhoI CTCGAG 2 cut(s) 244, 257
XmnI GAANNNNTTC 2 cut(s) 365, 994
XspI CTAG 4 cut(s) 999, 1496, 2070, 2144
ZraI GACGTC 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.