Rorug04G0358100

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001217
Physical Location & Seq
Reverse (-)
53113235 .. 53114308
1074 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0358100.1

Sequence Viewer

Length: 996 bp
ATGTTAGTGCCAACAAATGTAAAGGCCCCAACATCCTACATGACCCATGTTCGTATAGGACTAAAAGCATATCTGGAGCCGCCTAATGTTATGCACGACATGAATGATGAAGAATTGCTATGGAGAGCTTCGACGGCTCCTCGGATTTCTGAGTATCCATTCCATCGAGTCCCAAAAGTTGCTTTCATGTTCTTGACAAAGGGACCTGTACATTTGGCCCCATTGTGGGAGAAGTTCTTCAAAGGGCATCAAGGCTTGTACTCAATTTATGTTCACTCAGATCCATCTTACAATGCATCATCACACCCAGAAAGTCCTGTTTTTCAAGGCCGAAGAGTTCCGAGTCAGAAAGTAAAATGGGCACAGGTGAACATGATTGAGGCCGAAAGCCGCCTAATTGCGAATGCTCTTCTTGATATCTTAAACCAGCACTTCGTTCTCCTCTCGGAAGCATGCATTCCCCTATACAACTTCTCCACTATCTACTCTTACCTCCTAAACTCCCAAGAAACTTTTGTGGAGGTCTATGATGATCCAAGTTCAGTTGGACGTGGCCGATATAATTCAAGTTACTACCCAATAATCACATTGGATCAGTGGAGGAAAGGGTCACAGTGGGTTGAAATTGATAGAGACATTGCCATTGAAGTTGTCTCGGATCGGACATACTTACCAGCCTTCCGGTTGGGCAACCGCTCTTGTTTTGCTGATGAACATTACTTGCCAACATTTGTGAACCTAAAATTTTCGGCAAAGAATTCAAACAGGAGTGTCACCTGGGTTGATTGGTCTACGCGTGGCCCGCACCCTGTCGAGTACAATAGCACGTCGGCTGAGCTTCTGAACACCCTGCGAAATGGTAATGGTCAGAGATGTTTATATAATCGAAGGAGAAGCAATGTTTGTTTCTTATTCGCCAGGAAGTTCCGGCCTTCTGCTTTGGATAGCCTTCTCAGGCTTGCCCCCAAGATCATGCACTTCAACACAGATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

331

Amino Acids

38.21

Weight (kDa)

9.14

Isoelectric Point (pI)

49.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 60 - 283 6.8e-73 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 696
AccI GTMKAC 1 cut(s) 791
AccII CGCG 1 cut(s) 796
AciI CCGC 4 cut(s) 80, 391, 694, 803
AclWI GGATC 4 cut(s) 275, 527, 600, 666
AcoI YGGCCR 1 cut(s) 553
AcsI RAATTY 2 cut(s) 743, 757
AfaI GTAC 3 cut(s) 210, 260, 818
AfiI CCNNNNNNNGG 2 cut(s) 225, 226
AflIII ACRYGT 1 cut(s) 794
AgsI TTSAA 7 cut(s) 241, 326, 567, 623, 647, 762, 982
AjiI CACGTC 2 cut(s) 551, 828
AjnI CCWGG 2 cut(s) 776, 917
AluBI AGCT 2 cut(s) 128, 838
AluI AGCT 2 cut(s) 128, 838
Alw26I GTCTC 2 cut(s) 627, 658
AlwI GGATC 4 cut(s) 275, 527, 600, 666
AoxI GGCC 7 cut(s) 24, 216, 328, 381, 553, 799, 929
ApoI RAATTY 2 cut(s) 743, 757
Asp700I GAANNNNTTC 1 cut(s) 236
AspS9I GGNCC 4 cut(s) 25, 203, 217, 800
AsuHPI GGTGA 2 cut(s) 379, 766
AvaII GGWCC 1 cut(s) 203
BaeGI GKGCMC 1 cut(s) 364
BccI CCATC 2 cut(s) 171, 292
BceAI ACGGC 1 cut(s) 150
BciT130I CCWGG 2 cut(s) 778, 919
BciVI GTATCC 1 cut(s) 165
BcoDI GTCTC 2 cut(s) 627, 658
BfuI GTATCC 1 cut(s) 165
BisI GCNGC 2 cut(s) 80, 391
BlpI GCTNAGC 1 cut(s) 834
BlsI GCNGC 2 cut(s) 81, 392
Bme1390I CCNGG 2 cut(s) 778, 919
Bme18I GGWCC 1 cut(s) 203
BmgBI CACGTC 2 cut(s) 551, 828
BmgT120I GGNCC 4 cut(s) 25, 203, 217, 800
BmiI GGNNCC 5 cut(s) 27, 78, 138, 204, 219
BmrFI CCNGG 2 cut(s) 778, 919
BmsI GCATC 2 cut(s) 256, 305
BpmI CTGGAG 1 cut(s) 95
Bpu1102I GCTNAGC 1 cut(s) 834
BsaJI CCNNGG 2 cut(s) 140, 777
BsaWI WCCGGW 1 cut(s) 681
BsaXI ACNNNNNCTCC 2 cut(s) 458, 488
Bsc4I CCNNNNNNNGG 2 cut(s) 225, 226
Bse3DI GCAATG 2 cut(s) 636, 904
BseBI CCWGG 2 cut(s) 778, 919
BseDI CCNNGG 2 cut(s) 140, 777
BseGI GGATG 1 cut(s) 32
BseLI CCNNNNNNNGG 2 cut(s) 225, 226
BseMI GCAATG 2 cut(s) 636, 904
BseMII CTCAG 4 cut(s) 141, 291, 825, 967
BseRI GAGGAG 2 cut(s) 129, 431
BseSI GKGCMC 1 cut(s) 364
Bsh1236I CGCG 1 cut(s) 796
BshFI GGCC 7 cut(s) 26, 218, 330, 383, 555, 801, 931
BsiSI CCGG 2 cut(s) 682, 928
BslFI GGGAC 2 cut(s) 155, 216
BslI CCNNNNNNNGG 2 cut(s) 225, 226
BsmAI GTCTC 2 cut(s) 627, 658
BsmFI GGGAC 2 cut(s) 155, 216
BsmI GAATGC 2 cut(s) 409, 456
BsnI GGCC 7 cut(s) 26, 218, 330, 383, 555, 801, 931
Bsp1286I GDGCHC 1 cut(s) 364
Bsp1407I TGTACA 1 cut(s) 208
Bsp143I GATC 5 cut(s) 280, 532, 592, 658, 969
Bsp1720I GCTNAGC 1 cut(s) 834
BspACI CCGC 4 cut(s) 80, 391, 694, 803
BspANI GGCC 7 cut(s) 26, 218, 330, 383, 555, 801, 931
BspCNI CTCAG 4 cut(s) 142, 290, 826, 966
BspFNI CGCG 1 cut(s) 796
BspLI GGNNCC 5 cut(s) 27, 78, 138, 204, 219
BspPI GGATC 4 cut(s) 275, 527, 600, 666
BspQI GCTCTTC 1 cut(s) 414
BsrBI CCGCTC 1 cut(s) 696
BsrDI GCAATG 2 cut(s) 636, 904
BsrGI TGTACA 1 cut(s) 208
BssECI CCNNGG 2 cut(s) 140, 777
BssMI GATC 5 cut(s) 280, 532, 592, 658, 969
Bst2UI CCWGG 2 cut(s) 778, 919
Bst4CI ACNGT 1 cut(s) 615
Bst6I CTCTTC 2 cut(s) 328, 414
BstAUI TGTACA 1 cut(s) 208
BstC8I GCNNGC 3 cut(s) 454, 803, 960
BstDEI CTNAG 4 cut(s) 150, 277, 834, 953
BstF5I GGATG 1 cut(s) 32
BstFNI CGCG 1 cut(s) 796
BstKTI GATC 5 cut(s) 283, 535, 595, 661, 972
BstMAI GTCTC 2 cut(s) 627, 658
BstMBI GATC 5 cut(s) 280, 532, 592, 658, 969
BstMWI GCNNNNNNNGC 2 cut(s) 134, 802
BstNI CCWGG 2 cut(s) 778, 919
BstNSI RCATGY 1 cut(s) 456
BstSCI CCNGG 2 cut(s) 776, 917
BstSLI GKGCMC 1 cut(s) 364
BstUI CGCG 1 cut(s) 796
BstX2I RGATCY 1 cut(s) 280
BstYI RGATCY 1 cut(s) 280
BsuI GTATCC 1 cut(s) 165
BsuRI GGCC 7 cut(s) 26, 218, 330, 383, 555, 801, 931
BtrI CACGTC 2 cut(s) 551, 828
BtsCI GGATG 1 cut(s) 32
BtsIMutI CAGTG 2 cut(s) 602, 620
Cac8I GCNNGC 3 cut(s) 454, 803, 960
Cfr13I GGNCC 4 cut(s) 25, 203, 217, 800
Csp6I GTAC 3 cut(s) 209, 259, 817
CviAII CATG 7 cut(s) 40, 47, 100, 187, 373, 453, 973
CviQI GTAC 3 cut(s) 209, 259, 817
DdeI CTNAG 4 cut(s) 150, 277, 834, 953
DpnI GATC 5 cut(s) 282, 534, 594, 660, 971
DpnII GATC 5 cut(s) 280, 532, 592, 658, 969
EaeI YGGCCR 1 cut(s) 553
Eam1104I CTCTTC 2 cut(s) 328, 414
EarI CTCTTC 2 cut(s) 328, 414
Eco32I GATATC 1 cut(s) 418
Eco47I GGWCC 1 cut(s) 203
EcoO109I RGGNCCY 2 cut(s) 25, 203
EcoRI GAATTC 1 cut(s) 757
EcoRII CCWGG 2 cut(s) 776, 917
EcoRV GATATC 1 cut(s) 418
EcoT22I ATGCAT 2 cut(s) 298, 458
FaeI CATG 7 cut(s) 43, 50, 103, 190, 376, 456, 976
FaqI GGGAC 2 cut(s) 155, 216
FatI CATG 7 cut(s) 39, 46, 99, 186, 372, 452, 972
FauI CCCGC 1 cut(s) 810
FblI GTMKAC 1 cut(s) 791
Fnu4HI GCNGC 2 cut(s) 80, 391
FokI GGATG 1 cut(s) 19
Fsp4HI GCNGC 2 cut(s) 80, 391
GluI GCNGC 2 cut(s) 80, 391
GsuI CTGGAG 1 cut(s) 95
HaeIII GGCC 7 cut(s) 26, 218, 330, 383, 555, 801, 931
HapII CCGG 2 cut(s) 682, 928
Hin1II CATG 7 cut(s) 43, 50, 103, 190, 376, 456, 976
HinfI GANTC 3 cut(s) 168, 343, 989
HpaII CCGG 2 cut(s) 682, 928
HphI GGTGA 2 cut(s) 379, 766
Hpy166II GTNNAC 4 cut(s) 274, 370, 736, 792
Hpy188III TCNNGA 3 cut(s) 74, 193, 413
Hpy8I GTNNAC 4 cut(s) 274, 370, 736, 792
Hpy99I CGWCG 2 cut(s) 136, 832
HpyAV CCTTC 4 cut(s) 688, 882, 942, 959
HpyCH4III ACNGT 1 cut(s) 615
HpyCH4IV ACGT 2 cut(s) 550, 827
HpyCH4V TGCA 4 cut(s) 94, 296, 456, 976
HpyF10VI GCNNNNNNNGC 2 cut(s) 134, 802
HpyF3I CTNAG 4 cut(s) 150, 277, 834, 953
HpySE526I ACGT 2 cut(s) 550, 827
Hsp92II CATG 7 cut(s) 43, 50, 103, 190, 376, 456, 976
Kzo9I GATC 5 cut(s) 280, 532, 592, 658, 969
LguI GCTCTTC 1 cut(s) 414
LmnI GCTCC 2 cut(s) 76, 142
LweI GCATC 2 cut(s) 256, 305
MaeII ACGT 2 cut(s) 550, 827
MaeIII GTNAC 3 cut(s) 569, 609, 772
MalI GATC 5 cut(s) 282, 534, 594, 660, 971
MbiI CCGCTC 1 cut(s) 696
MboI GATC 5 cut(s) 280, 532, 592, 658, 969
MboII GAAGA 4 cut(s) 122, 229, 345, 401
MflI RGATCY 1 cut(s) 280
MhlI GDGCHC 1 cut(s) 364
MluCI AATT 7 cut(s) 113, 264, 396, 562, 624, 743, 757
MluI ACGCGT 1 cut(s) 794
MlyI GAGTC 2 cut(s) 177, 352
MmeI TCCRAC 1 cut(s) 526
MnlI CCTC 6 cut(s) 150, 373, 452, 503, 514, 594
Mph1103I ATGCAT 2 cut(s) 298, 458
MroXI GAANNNNTTC 1 cut(s) 236
MseI TTAA 2 cut(s) 422, 994
MspI CCGG 2 cut(s) 682, 928
MspR9I CCNGG 2 cut(s) 778, 919
Mva1269I GAATGC 2 cut(s) 409, 456
MvaI CCWGG 2 cut(s) 778, 919
MvnI CGCG 1 cut(s) 796
MwoI GCNNNNNNNGC 2 cut(s) 134, 802
NdeII GATC 5 cut(s) 280, 532, 592, 658, 969
NlaIII CATG 7 cut(s) 43, 50, 103, 190, 376, 456, 976
NlaIV GGNNCC 5 cut(s) 27, 78, 138, 204, 219
NmuCI GTSAC 2 cut(s) 609, 772
NsiI ATGCAT 2 cut(s) 298, 458
NspI RCATGY 1 cut(s) 456
PaeI GCATGC 1 cut(s) 456
PciSI GCTCTTC 1 cut(s) 414
PctI GAATGC 2 cut(s) 409, 456
PdmI GAANNNNTTC 1 cut(s) 236
PfeI GAWTC 1 cut(s) 989
PkrI GCNGC 2 cut(s) 81, 392
PleI GAGTC 2 cut(s) 176, 351
PpsI GAGTC 2 cut(s) 176, 351
PpuMI RGGWCCY 1 cut(s) 203
Psp5II RGGWCCY 1 cut(s) 203
Psp6I CCWGG 2 cut(s) 776, 917
PspGI CCWGG 2 cut(s) 776, 917
PspN4I GGNNCC 5 cut(s) 27, 78, 138, 204, 219
PspPI GGNCC 4 cut(s) 25, 203, 217, 800
PspPPI RGGWCCY 1 cut(s) 203
PsuI RGATCY 1 cut(s) 280
RsaI GTAC 3 cut(s) 210, 260, 818
RsaNI GTAC 3 cut(s) 209, 259, 817
SapI GCTCTTC 1 cut(s) 414
SaqAI TTAA 2 cut(s) 422, 994
SatI GCNGC 2 cut(s) 80, 391
Sau3AI GATC 5 cut(s) 280, 532, 592, 658, 969
Sau96I GGNCC 4 cut(s) 25, 203, 217, 800
SchI GAGTC 2 cut(s) 177, 352
ScrFI CCNGG 2 cut(s) 778, 919
SduI GDGCHC 1 cut(s) 364
SfaNI GCATC 2 cut(s) 256, 305
SinI GGWCC 1 cut(s) 203
SphI GCATGC 1 cut(s) 456
Sse9I AATT 7 cut(s) 113, 264, 396, 562, 624, 743, 757
SsiI CCGC 4 cut(s) 80, 391, 694, 803
StyD4I CCNGG 2 cut(s) 776, 917
TaaI ACNGT 1 cut(s) 615
TaiI ACGT 2 cut(s) 553, 830
TaqI TCGA 4 cut(s) 131, 166, 813, 886
TasI AATT 7 cut(s) 113, 264, 396, 562, 624, 743, 757
TatI WGTACW 3 cut(s) 208, 258, 816
TauI GCSGC 2 cut(s) 82, 393
TfiI GAWTC 1 cut(s) 989
Tru1I TTAA 2 cut(s) 422, 994
Tru9I TTAA 2 cut(s) 422, 994
TscAI CASTG 2 cut(s) 602, 620
TseFI GTSAC 2 cut(s) 609, 772
Tsp45I GTSAC 2 cut(s) 609, 772
TspDTI ATGAA 4 cut(s) 116, 123, 175, 726
TspRI CASTG 2 cut(s) 602, 620
VpaK11BI GGWCC 1 cut(s) 203
XapI RAATTY 2 cut(s) 743, 757
XceI RCATGY 1 cut(s) 456
XmiI GTMKAC 1 cut(s) 791
XmnI GAANNNNTTC 1 cut(s) 236
Zsp2I ATGCAT 2 cut(s) 298, 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.