Rorug04G0358400

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001217
Physical Location & Seq
Reverse (-)
53122099 .. 53123269
1171 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0358400.1

Sequence Viewer

Length: 1098 bp
ATGTTCATTATCGATCACACTACGAAAGTCTTAAGTTCAGAGGTCTACTTCATCCCCCAGCTGAAGACATTTTCCCCACTCACAGCTAATTGCACTAGCCACTTTTCTGCGTCTCCGCAGTTACTCCCTATTAGTTCTCTTCCAAATATAAGAGATTACATAGCTCCCAAAGAGCTATGGCACTCCATGAGTGATAAGGAGCTAATATGGCGAGCCTCTATGGTGCCACAGGTCACCGAATACCCTTATAATCGAACGCCAAAGGTCGCATTCATGTTCCTTGCAAAGGGAAAACTACCATTGGTGCCTCTTTGGGAAAGGTTTTTCATGGGACATGAAGGCTTCTTCTCAGTTTATCTCCATGCAGCACCGGACTTTAAGAACGAGCCCCCCGAATCCTCAGTGTTTTACAATCGGAGGATACCAAGTAAGGCAGTTGAATGGGGAAAGGCCACAATGGTTGAAGCAGAGAGGCGCCTATTAGCCAATGCCTTACTTGACTTCTCAAATGAGAGATTTGTACTGCTCTCTGAATCATGCATACCTCTCTTCAACTTCACCACAATCTATGACTACCTCATCAACTCCAACTACAGTTTCATTAGCTCATTCGATGACCCGAGAGGCACCGGCCGGGGGCGCTACAACAGGCGAATGTGGCCAACCGTGACATTGTCCGATTGGCGCAAGGGTTCACAATGGTTCGAAGTTCACAGAAACCTTGCCCTGAAGATAGTATCAGATGTAACATACTACCCTATATTTAAGGACCTCTGCCAGCCTCCTTGCTACAACGATGAACACTATTTGGCAACTCTGGTGAGCAAAGTTGGCCCCGGCATGAACTCAAATAGGAGCATTACCTGGGTTGATTGGTCCGTAGGTGGCCCGCACCCCGCAACATATGGGAGGCAACATGTGACAGAAGCGTTTCTGAATCAGTTGAGACATGGGTTTGATTGTACCTACAATGGTGGCATTAGTAACATTTGCCACCTCTTTGCTAGGAAATTTCATCCTAGTACACTTGAGCCTCTGCTAAAAATAGCTCCGACTCTGTTTGGATTTAATACCGATAATATTACCACTACAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

365

Amino Acids

42.0

Weight (kDa)

8.77

Isoelectric Point (pI)

38.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 89 - 316 1.5e-78 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 249
AccB1I GGYRCC 4 cut(s) 223, 304, 474, 626
AccI GTMKAC 1 cut(s) 45
AciI CCGC 3 cut(s) 116, 890, 897
AcoI YGGCCR 2 cut(s) 631, 659
AcsI RAATTY 1 cut(s) 1010
AcuI CTGAAG 2 cut(s) 83, 749
AcyI GRCGYC 1 cut(s) 475
AfaI GTAC 3 cut(s) 522, 964, 1024
AfiI CCNNNNNNNGG 2 cut(s) 286, 636
AflII CTTAAG 1 cut(s) 31
AflIII ACRYGT 1 cut(s) 916
AgsI TTSAA 3 cut(s) 440, 464, 553
AjnI CCWGG 1 cut(s) 863
AjuI GAANNNNNNNTTGG 2 cut(s) 581, 613
AluBI AGCT 7 cut(s) 61, 86, 164, 175, 202, 606, 1049
AluI AGCT 7 cut(s) 61, 86, 164, 175, 202, 606, 1049
Alw26I GTCTC 2 cut(s) 117, 940
Ama87I CYCGRG 1 cut(s) 619
AoxI GGCC 5 cut(s) 450, 631, 659, 832, 886
ApeKI GCWGC 1 cut(s) 365
ApoI RAATTY 1 cut(s) 1010
Asp700I GAANNNNTTC 1 cut(s) 930
AspLEI GCGC 3 cut(s) 477, 642, 687
AspS9I GGNCC 4 cut(s) 769, 833, 876, 887
AsuC2I CCSGG 2 cut(s) 635, 837
AsuHPI GGTGA 3 cut(s) 226, 550, 832
AsuII TTCGAA 1 cut(s) 705
AvaI CYCGRG 1 cut(s) 619
AvaII GGWCC 2 cut(s) 769, 876
BalI TGGCCA 1 cut(s) 661
BanI GGYRCC 4 cut(s) 223, 304, 474, 626
BanII GRGCYC 1 cut(s) 390
BbsI GAAGAC 1 cut(s) 71
BbvI GCAGC 1 cut(s) 377
BciT130I CCWGG 1 cut(s) 865
BciVI GTATCC 1 cut(s) 414
BcnI CCSGG 2 cut(s) 635, 837
BcoDI GTCTC 2 cut(s) 117, 940
BfaI CTAG 3 cut(s) 96, 1005, 1020
BfmI CTRYAG 1 cut(s) 592
BfoI RGCGCY 2 cut(s) 478, 643
BfrI CTTAAG 1 cut(s) 31
BfuI GTATCC 1 cut(s) 414
BisI GCNGC 1 cut(s) 366
BlsI GCNGC 1 cut(s) 367
Bme1390I CCNGG 3 cut(s) 635, 837, 865
Bme18I GGWCC 2 cut(s) 769, 876
BmeT110I CYCGRG 1 cut(s) 619
BmgT120I GGNCC 4 cut(s) 769, 833, 876, 887
BmiI GGNNCC 5 cut(s) 225, 306, 476, 628, 835
BmrFI CCNGG 3 cut(s) 635, 837, 865
BpiI GAAGAC 1 cut(s) 71
Bpu14I TTCGAA 1 cut(s) 705
BpuEI CTTGAG 1 cut(s) 1049
BpuMI CCSGG 2 cut(s) 635, 837
Bsa29I ATCGAT 1 cut(s) 12
BsaHI GRCGYC 1 cut(s) 475
BsaJI CCNNGG 3 cut(s) 634, 835, 864
BsaWI WCCGGW 1 cut(s) 370
Bsc4I CCNNNNNNNGG 2 cut(s) 286, 636
Bse118I RCCGGY 1 cut(s) 629
BseBI CCWGG 1 cut(s) 865
BseCI ATCGAT 1 cut(s) 12
BseDI CCNNGG 3 cut(s) 634, 835, 864
BseGI GGATG 2 cut(s) 51, 1015
BseLI CCNNNNNNNGG 2 cut(s) 286, 636
BseMII CTCAG 2 cut(s) 363, 414
BseX3I CGGCCG 1 cut(s) 631
BseXI GCAGC 1 cut(s) 377
BseYI CCCAGC 1 cut(s) 57
Bsh1285I CGRYCG 1 cut(s) 634
BshFI GGCC 5 cut(s) 452, 633, 661, 834, 888
BshNI GGYRCC 4 cut(s) 223, 304, 474, 626
BshVI ATCGAT 1 cut(s) 12
BsiEI CGRYCG 1 cut(s) 634
BsiHKCI CYCGRG 1 cut(s) 619
BsiSI CCGG 4 cut(s) 371, 630, 634, 837
BslFI GGGAC 1 cut(s) 345
BslI CCNNNNNNNGG 2 cut(s) 286, 636
BsmAI GTCTC 2 cut(s) 117, 940
BsmBI CGTCTC 1 cut(s) 117
BsmFI GGGAC 1 cut(s) 345
BsmI GAATGC 1 cut(s) 269
BsnI GGCC 5 cut(s) 452, 633, 661, 834, 888
BsoBI CYCGRG 1 cut(s) 619
Bsp119I TTCGAA 1 cut(s) 705
Bsp1286I GDGCHC 1 cut(s) 390
Bsp143I GATC 1 cut(s) 13
BspACI CCGC 3 cut(s) 116, 890, 897
BspANI GGCC 5 cut(s) 452, 633, 661, 834, 888
BspCNI CTCAG 2 cut(s) 362, 413
BspDI ATCGAT 1 cut(s) 12
BspLI GGNNCC 5 cut(s) 225, 306, 476, 628, 835
BspT104I TTCGAA 1 cut(s) 705
BspT107I GGYRCC 4 cut(s) 223, 304, 474, 626
BspTI CTTAAG 1 cut(s) 31
BsrFI RCCGGY 1 cut(s) 629
BssAI RCCGGY 1 cut(s) 629
BssECI CCNNGG 3 cut(s) 634, 835, 864
BssMI GATC 1 cut(s) 13
BssNI GRCGYC 1 cut(s) 475
Bst2UI CCWGG 1 cut(s) 865
Bst4CI ACNGT 2 cut(s) 596, 667
Bst6I CTCTTC 2 cut(s) 144, 554
BstACI GRCGYC 1 cut(s) 475
BstAFI CTTAAG 1 cut(s) 31
BstBI TTCGAA 1 cut(s) 705
BstC8I GCNNGC 3 cut(s) 213, 779, 890
BstDEI CTNAG 2 cut(s) 349, 400
BstEII GGTNACC 1 cut(s) 232
BstENI CCTNNNNNAGG 1 cut(s) 284
BstF5I GGATG 2 cut(s) 51, 1015
BstH2I RGCGCY 2 cut(s) 478, 643
BstHHI GCGC 3 cut(s) 477, 642, 687
BstKTI GATC 1 cut(s) 16
BstMAI GTCTC 2 cut(s) 117, 940
BstMBI GATC 1 cut(s) 13
BstMCI CGRYCG 1 cut(s) 634
BstMWI GCNNNNNNNGC 4 cut(s) 208, 639, 658, 831
BstNI CCWGG 1 cut(s) 865
BstNSI RCATGY 1 cut(s) 920
BstPI GGTNACC 1 cut(s) 232
BstSCI CCNGG 3 cut(s) 633, 835, 863
BstSFI CTRYAG 1 cut(s) 592
BstV1I GCAGC 1 cut(s) 377
BstV2I GAAGAC 1 cut(s) 71
BstZI CGGCCG 1 cut(s) 631
Bsu15I ATCGAT 1 cut(s) 12
BsuI GTATCC 1 cut(s) 414
BsuRI GGCC 5 cut(s) 452, 633, 661, 834, 888
BsuTUI ATCGAT 1 cut(s) 12
BtsCI GGATG 2 cut(s) 51, 1015
BtsIMutI CAGTG 1 cut(s) 408
Cac8I GCNNGC 3 cut(s) 213, 779, 890
CfoI GCGC 3 cut(s) 477, 642, 687
Cfr10I RCCGGY 1 cut(s) 629
Cfr13I GGNCC 4 cut(s) 769, 833, 876, 887
ClaI ATCGAT 1 cut(s) 12
CseI GACGC 1 cut(s) 99
Csp6I GTAC 3 cut(s) 521, 963, 1023
CspCI CAANNNNNGTGG 2 cut(s) 442, 477
CviAII CATG 9 cut(s) 187, 274, 328, 335, 362, 537, 841, 917, 950
CviQI GTAC 3 cut(s) 521, 963, 1023
DdeI CTNAG 2 cut(s) 349, 400
DinI GGCGCC 1 cut(s) 476
DpnI GATC 1 cut(s) 15
DpnII GATC 1 cut(s) 13
EaeI YGGCCR 2 cut(s) 631, 659
EagI CGGCCG 1 cut(s) 631
Eam1104I CTCTTC 2 cut(s) 144, 554
EarI CTCTTC 2 cut(s) 144, 554
EclXI CGGCCG 1 cut(s) 631
Eco24I GRGCYC 1 cut(s) 390
Eco47I GGWCC 2 cut(s) 769, 876
Eco52I CGGCCG 1 cut(s) 631
Eco57I CTGAAG 2 cut(s) 83, 749
Eco88I CYCGRG 1 cut(s) 619
Eco91I GGTNACC 1 cut(s) 232
EcoNI CCTNNNNNAGG 1 cut(s) 284
EcoO109I RGGNCCY 1 cut(s) 769
EcoO65I GGTNACC 1 cut(s) 232
EcoRII CCWGG 1 cut(s) 863
EcoT22I ATGCAT 1 cut(s) 542
EcoT38I GRGCYC 1 cut(s) 390
EgeI GGCGCC 1 cut(s) 476
EheI GGCGCC 1 cut(s) 476
Esp3I CGTCTC 1 cut(s) 117
FaeI CATG 9 cut(s) 190, 277, 331, 338, 365, 540, 844, 920, 953
FaqI GGGAC 1 cut(s) 345
FatI CATG 9 cut(s) 186, 273, 327, 334, 361, 536, 840, 916, 949
FauI CCCGC 2 cut(s) 897, 904
FauNDI CATATG 1 cut(s) 904
FblI GTMKAC 1 cut(s) 45
Fnu4HI GCNGC 1 cut(s) 366
FokI GGATG 2 cut(s) 38, 1002
FriOI GRGCYC 1 cut(s) 390
Fsp4HI GCNGC 1 cut(s) 366
FspBI CTAG 3 cut(s) 96, 1005, 1020
GlaI GCGC 3 cut(s) 476, 641, 686
GluI GCNGC 1 cut(s) 366
GsaI CCCAGC 1 cut(s) 61
HaeII RGCGCY 2 cut(s) 478, 643
HaeIII GGCC 5 cut(s) 452, 633, 661, 834, 888
HapII CCGG 4 cut(s) 371, 630, 634, 837
HgaI GACGC 1 cut(s) 99
HhaI GCGC 3 cut(s) 477, 642, 687
Hin1I GRCGYC 1 cut(s) 475
Hin1II CATG 9 cut(s) 190, 277, 331, 338, 365, 540, 844, 920, 953
Hin6I GCGC 3 cut(s) 475, 640, 685
HinP1I GCGC 3 cut(s) 475, 640, 685
HinfI GANTC 4 cut(s) 395, 533, 937, 1054
HpaII CCGG 4 cut(s) 371, 630, 634, 837
HphI GGTGA 3 cut(s) 226, 550, 832
Hpy166II GTNNAC 4 cut(s) 46, 695, 712, 1025
Hpy188I TCNGA 7 cut(s) 40, 417, 532, 679, 742, 936, 1053
Hpy8I GTNNAC 4 cut(s) 46, 695, 712, 1025
HpyAV CCTTC 1 cut(s) 332
HpyCH4III ACNGT 2 cut(s) 596, 667
HpyCH4V TGCA 4 cut(s) 93, 284, 365, 540
HpyF10VI GCNNNNNNNGC 4 cut(s) 208, 639, 658, 831
HpyF3I CTNAG 2 cut(s) 349, 400
Hsp92I GRCGYC 1 cut(s) 475
Hsp92II CATG 9 cut(s) 190, 277, 331, 338, 365, 540, 844, 920, 953
HspAI GCGC 3 cut(s) 475, 640, 685
KasI GGCGCC 1 cut(s) 474
Kzo9I GATC 1 cut(s) 13
LmnI GCTCC 4 cut(s) 169, 199, 855, 1054
Lsp1109I GCAGC 1 cut(s) 377
MaeI CTAG 3 cut(s) 96, 1005, 1020
MaeIII GTNAC 6 cut(s) 120, 232, 667, 745, 919, 983
MalI GATC 1 cut(s) 15
MboI GATC 1 cut(s) 13
MboII GAAGA 5 cut(s) 76, 131, 337, 541, 742
MhlI GDGCHC 1 cut(s) 390
MlsI TGGCCA 1 cut(s) 661
MluCI AATT 2 cut(s) 88, 1010
MluNI TGGCCA 1 cut(s) 661
Mly113I GGCGCC 1 cut(s) 475
MlyI GAGTC 1 cut(s) 1048
MmeI TCCRAC 2 cut(s) 612, 1076
Mox20I TGGCCA 1 cut(s) 661
Mph1103I ATGCAT 1 cut(s) 542
MroXI GAANNNNTTC 1 cut(s) 930
MscI TGGCCA 1 cut(s) 661
MseI TTAA 4 cut(s) 32, 378, 765, 1068
Msp20I TGGCCA 1 cut(s) 661
MspA1I CMGCKG 1 cut(s) 61
MspCI CTTAAG 1 cut(s) 31
MspI CCGG 4 cut(s) 371, 630, 634, 837
MspR9I CCNGG 3 cut(s) 635, 837, 865
Mva1269I GAATGC 1 cut(s) 269
MvaI CCWGG 1 cut(s) 865
MwoI GCNNNNNNNGC 4 cut(s) 208, 639, 658, 831
NarI GGCGCC 1 cut(s) 475
NciI CCSGG 2 cut(s) 635, 837
NdeI CATATG 1 cut(s) 904
NdeII GATC 1 cut(s) 13
NlaIII CATG 9 cut(s) 190, 277, 331, 338, 365, 540, 844, 920, 953
NlaIV GGNNCC 5 cut(s) 225, 306, 476, 628, 835
NmuCI GTSAC 3 cut(s) 232, 667, 919
NsiI ATGCAT 1 cut(s) 542
NspI RCATGY 1 cut(s) 920
NspV TTCGAA 1 cut(s) 705
PciI ACATGT 1 cut(s) 916
PcsI WCGNNNNNNNCGW 1 cut(s) 390
PctI GAATGC 1 cut(s) 269
PdmI GAANNNNTTC 1 cut(s) 930
PfeI GAWTC 3 cut(s) 395, 533, 937
PflFI GACNNNGTC 1 cut(s) 673
PkrI GCNGC 1 cut(s) 367
PleI GAGTC 1 cut(s) 1048
PluTI GGCGCC 1 cut(s) 478
PpsI GAGTC 1 cut(s) 1048
PpuMI RGGWCCY 1 cut(s) 769
PscI ACATGT 1 cut(s) 916
PsiI TTATAA 1 cut(s) 249
Psp5II RGGWCCY 1 cut(s) 769
Psp6I CCWGG 1 cut(s) 863
PspEI GGTNACC 1 cut(s) 232
PspFI CCCAGC 1 cut(s) 57
PspGI CCWGG 1 cut(s) 863
PspN4I GGNNCC 5 cut(s) 225, 306, 476, 628, 835
PspPI GGNCC 4 cut(s) 769, 833, 876, 887
PspPPI RGGWCCY 1 cut(s) 769
PsyI GACNNNGTC 1 cut(s) 673
PvuII CAGCTG 1 cut(s) 61
RsaI GTAC 3 cut(s) 522, 964, 1024
RsaNI GTAC 3 cut(s) 521, 963, 1023
SaqAI TTAA 4 cut(s) 32, 378, 765, 1068
SatI GCNGC 1 cut(s) 366
Sau3AI GATC 1 cut(s) 13
Sau96I GGNCC 4 cut(s) 769, 833, 876, 887
SchI GAGTC 1 cut(s) 1048
ScrFI CCNGG 3 cut(s) 635, 837, 865
SduI GDGCHC 1 cut(s) 390
SfcI CTRYAG 1 cut(s) 592
SfoI GGCGCC 1 cut(s) 476
SfuI TTCGAA 1 cut(s) 705
SinI GGWCC 2 cut(s) 769, 876
SmlI CTYRAG 2 cut(s) 31, 1028
SmoI CTYRAG 2 cut(s) 31, 1028
Sse9I AATT 2 cut(s) 88, 1010
SsiI CCGC 3 cut(s) 116, 890, 897
SspDI GGCGCC 1 cut(s) 474
SspI AATATT 1 cut(s) 1081
SspMI CTAG 3 cut(s) 96, 1005, 1020
StyD4I CCNGG 3 cut(s) 633, 835, 863
TaaI ACNGT 2 cut(s) 596, 667
TaqI TCGA 4 cut(s) 12, 253, 612, 705
TasI AATT 2 cut(s) 88, 1010
TatI WGTACW 2 cut(s) 520, 1022
TfiI GAWTC 3 cut(s) 395, 533, 937
Tru1I TTAA 4 cut(s) 32, 378, 765, 1068
Tru9I TTAA 4 cut(s) 32, 378, 765, 1068
TscAI CASTG 1 cut(s) 408
TseFI GTSAC 3 cut(s) 232, 667, 919
TseI GCWGC 1 cut(s) 365
Tsp45I GTSAC 3 cut(s) 232, 667, 919
TspDTI ATGAA 8 cut(s) 40, 262, 316, 351, 589, 813, 857, 1004
TspGWI ACGGA 1 cut(s) 868
TspRI CASTG 1 cut(s) 408
Tth111I GACNNNGTC 1 cut(s) 673
Vha464I CTTAAG 1 cut(s) 31
VpaK11BI GGWCC 2 cut(s) 769, 876
XagI CCTNNNNNAGG 1 cut(s) 284
XapI RAATTY 1 cut(s) 1010
XceI RCATGY 1 cut(s) 920
XmiI GTMKAC 1 cut(s) 45
XmnI GAANNNNTTC 1 cut(s) 930
XspI CTAG 3 cut(s) 96, 1005, 1020
Zsp2I ATGCAT 1 cut(s) 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.