pycom16g07150

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0001217
Physical Location & Seq
Reverse (-)
4657778 .. 4663450
5673 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g07150.4

Sequence Viewer

Length: 1179 bp
ATGGCCAAAGTCGGTGGCAAAGCCGGTTACGTTTGCAACTCCTCCAGCAAGGAAGCAGAGGTGGTTCAGGGGTTGGTCAAAAAGGTTTTGAATGAAATAAGGAAAACCCCGGTAGGTCTTGCTTCCTACACAGTGGGACTTGATTCTCGAGTTGAAGATGTGATGAGATTGTTAGATGTTCGATCCAATGGCGTTCAAGTGCTCGGAATTCATGGCATGGGTGGTGTTGGCAAGACGACTCTTGCGAAGGCCCTTTTTAACAGACTTGTTGCTGACTTTGCGTATCGCGGCTTCATCTCAAATGTTACAGAAACTTCTGCAGGACACGAGAACAGACTTATCGGCAGTCTTTCTACTCTGAAGATGCCGGTAAATGAACTTAACGCTGGTATTTCTGCAATAAAAGCGACTGTGCACGAGAAGCGAGTTCTTATTGTTTTGGATGATGTTGATAATGTAGAGCAACTTAGTGCTTTAGTTGGCAGCAGACACTGGTTTTATGAAGGAAGCAGAATCATTGTTACCACAAGAGATAGAAGTGCCTTGCCTGTTCATCTTGTGAACAAGTTGTATGAGGTCAGGGAGTTGGATTCGTCTCAGGCGCTTGAACTGTTTAGCTACCATGCACTGCGAAAAGAAAAGCCCCCTGGTGATTTCCTGGCGCTGTCCGAGCAGATTGTGTCTCTCACAGGTGGGCTACCGTTGGCTCTTGAAGTATTCGGCTCTTACCTATTATATCGGAGGAGAATAGAGGAATGGAGAGACAGTCTGCAGAAACTGAAACATATTCGTCCCGGCAATCTTCAAGATGTGTTAAAGATAAGTTATGATGCTCTAGACGAGCAAGAGAAGTGTATCTTCCTTGACTTTGCATGTTTATTTGTGAAAATGAATATGAAGAGAGAAGATGCCATTGACATACTGAAGGGTTGTGGTTTTGATGGTGAGATAGCAATTGCTGACCTCACCGCAAAATCTCTCATGAAGGTTTATGAAGACGGTATGCTGTGGATGCACGATCAAGTTAGAGACATGGGAAGACAAATCGTTAGACATGAAAGCGTTGTAGATCCTGGCATGCGTAGTAGACTTTGGGATCGTGATGAAATCTTGAATGTCTTCAAGGATGACAAGGGAACAAGATGTATACAAGGGATAGTCTTAGACTTCGAATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

393

Amino Acids

43.89

Weight (kDa)

6.53

Isoelectric Point (pI)

38.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 1087, 1147
AccII CGCG 1 cut(s) 288
AciI CCGC 2 cut(s) 288, 969
AclWI GGATC 3 cut(s) 177, 1064, 1104
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 207
AcuI CTGAAG 2 cut(s) 380, 944
AgsI TTSAA 8 cut(s) 91, 155, 197, 608, 713, 806, 1114, 1123
AjnI CCWGG 3 cut(s) 646, 657, 1072
AluBI AGCT 1 cut(s) 618
AluI AGCT 1 cut(s) 618
Alw21I GWGCWC 2 cut(s) 204, 417
Alw26I GTCTC 4 cut(s) 600, 687, 756, 1023
Alw44I GTGCAC 1 cut(s) 413
AlwI GGATC 3 cut(s) 177, 1064, 1104
AlwNI CAGNNNCTG 2 cut(s) 492, 778
Ama87I CYCGRG 1 cut(s) 147
AoxI GGCC 2 cut(s) 3, 249
ApaLI GTGCAC 1 cut(s) 413
ApeKI GCWGC 1 cut(s) 483
ApoI RAATTY 1 cut(s) 207
Asp700I GAANNNNTTC 1 cut(s) 1118
AspLEI GCGC 2 cut(s) 604, 664
AspS9I GGNCC 1 cut(s) 250
AsuC2I CCSGG 2 cut(s) 110, 795
AsuHPI GGTGA 3 cut(s) 662, 956, 958
AsuII TTCGAA 1 cut(s) 1170
AvaI CYCGRG 1 cut(s) 147
BaeGI GKGCMC 1 cut(s) 417
BalI TGGCCA 1 cut(s) 5
BarI GAAGNNNNNNTAC 2 cut(s) 275, 307
BauI CACGAG 2 cut(s) 326, 416
BbsI GAAGAC 3 cut(s) 1002, 1045, 1111
Bbv12I GWGCWC 2 cut(s) 204, 417
BbvI GCAGC 1 cut(s) 495
BccI CCATC 1 cut(s) 935
BciT130I CCWGG 3 cut(s) 648, 659, 1074
BcnI CCSGG 2 cut(s) 110, 795
BcoDI GTCTC 4 cut(s) 600, 687, 756, 1023
BfaI CTAG 1 cut(s) 836
BfmI CTRYAG 2 cut(s) 318, 770
BfoI RGCGCY 2 cut(s) 605, 665
BisI GCNGC 2 cut(s) 289, 484
BlsI GCNGC 2 cut(s) 290, 485
Bme1390I CCNGG 5 cut(s) 110, 648, 659, 795, 1074
BmeT110I CYCGRG 1 cut(s) 147
BmgT120I GGNCC 1 cut(s) 250
BmrFI CCNGG 5 cut(s) 110, 648, 659, 795, 1074
BmsI GCATC 4 cut(s) 354, 820, 898, 1002
BpiI GAAGAC 3 cut(s) 1002, 1045, 1111
BpmI CTGGAG 1 cut(s) 28
Bpu14I TTCGAA 1 cut(s) 1170
BpuMI CCSGG 2 cut(s) 110, 795
BsaJI CCNNGG 2 cut(s) 108, 646
BsaXI ACNNNNNCTCC 2 cut(s) 751, 781
Bse118I RCCGGY 2 cut(s) 23, 367
Bse1I ACTGG 1 cut(s) 497
BseBI CCWGG 3 cut(s) 648, 659, 1074
BseDI CCNNGG 2 cut(s) 108, 646
BseGI GGATG 3 cut(s) 448, 1017, 1132
BseMII CTCAG 1 cut(s) 611
BseNI ACTGG 1 cut(s) 497
BseRI GAGGAG 2 cut(s) 31, 757
BseSI GKGCMC 1 cut(s) 417
BseXI GCAGC 1 cut(s) 495
Bsh1236I CGCG 1 cut(s) 288
BshFI GGCC 2 cut(s) 5, 251
BsiHKAI GWGCWC 2 cut(s) 204, 417
BsiHKCI CYCGRG 1 cut(s) 147
BsiSI CCGG 4 cut(s) 24, 110, 368, 795
BslFI GGGAC 2 cut(s) 150, 777
BsmAI GTCTC 4 cut(s) 600, 687, 756, 1023
BsmBI CGTCTC 1 cut(s) 600
BsmFI GGGAC 2 cut(s) 150, 777
BsnI GGCC 2 cut(s) 5, 251
BsoBI CYCGRG 1 cut(s) 147
Bsp119I TTCGAA 1 cut(s) 1170
Bsp1286I GDGCHC 2 cut(s) 204, 417
Bsp143I GATC 4 cut(s) 182, 1018, 1069, 1096
BspACI CCGC 2 cut(s) 288, 969
BspANI GGCC 2 cut(s) 5, 251
BspCNI CTCAG 1 cut(s) 610
BspFNI CGCG 1 cut(s) 288
BspHI TCATGA 1 cut(s) 981
BspMAI CTGCAG 2 cut(s) 322, 774
BspPI GGATC 3 cut(s) 177, 1064, 1104
BspT104I TTCGAA 1 cut(s) 1170
BsrFI RCCGGY 2 cut(s) 23, 367
BsrI ACTGG 1 cut(s) 497
BssAI RCCGGY 2 cut(s) 23, 367
BssECI CCNNGG 2 cut(s) 108, 646
BssMI GATC 4 cut(s) 182, 1018, 1069, 1096
BssNAI GTATAC 1 cut(s) 1148
BssSI CACGAG 2 cut(s) 326, 416
Bst1107I GTATAC 1 cut(s) 1148
Bst2BI CACGAG 2 cut(s) 326, 416
Bst2UI CCWGG 3 cut(s) 648, 659, 1074
Bst4CI ACNGT 6 cut(s) 133, 412, 612, 702, 767, 1001
Bst6I CTCTTC 1 cut(s) 893
BstBI TTCGAA 1 cut(s) 1170
BstC8I GCNNGC 1 cut(s) 1079
BstDEI CTNAG 3 cut(s) 467, 597, 1162
BstF5I GGATG 3 cut(s) 448, 1017, 1132
BstFNI CGCG 1 cut(s) 288
BstH2I RGCGCY 2 cut(s) 605, 665
BstHHI GCGC 2 cut(s) 604, 664
BstKTI GATC 4 cut(s) 185, 1021, 1072, 1099
BstMAI GTCTC 4 cut(s) 600, 687, 756, 1023
BstMBI GATC 4 cut(s) 182, 1018, 1069, 1096
BstMWI GCNNNNNNNGC 5 cut(s) 278, 404, 421, 670, 1012
BstNI CCWGG 3 cut(s) 648, 659, 1074
BstNSI RCATGY 2 cut(s) 876, 1081
BstSCI CCNGG 5 cut(s) 108, 646, 657, 793, 1072
BstSFI CTRYAG 2 cut(s) 318, 770
BstSLI GKGCMC 1 cut(s) 417
BstUI CGCG 1 cut(s) 288
BstV1I GCAGC 1 cut(s) 495
BstV2I GAAGAC 3 cut(s) 1002, 1045, 1111
BstX2I RGATCY 1 cut(s) 1069
BstYI RGATCY 1 cut(s) 1069
BstZ17I GTATAC 1 cut(s) 1148
BsuRI GGCC 2 cut(s) 5, 251
BtsCI GGATG 3 cut(s) 448, 1017, 1132
BtsI GCAGTG 1 cut(s) 626
BtsIMutI CAGTG 3 cut(s) 138, 490, 626
Cac8I GCNNGC 1 cut(s) 1079
CaiI CAGNNNCTG 2 cut(s) 492, 778
CciI TCATGA 1 cut(s) 981
CfoI GCGC 2 cut(s) 604, 664
Cfr10I RCCGGY 2 cut(s) 23, 367
Cfr13I GGNCC 1 cut(s) 250
CspCI CAANNNNNGTGG 1 cut(s) 30
CviAII CATG 8 cut(s) 212, 217, 623, 873, 982, 1033, 1055, 1078
CviJI RGCY 9 cut(s) 5, 23, 251, 291, 618, 643, 697, 707, 723
CviKI_1 RGCY 9 cut(s) 5, 23, 251, 291, 618, 643, 697, 707, 723
DdeI CTNAG 3 cut(s) 467, 597, 1162
DpnI GATC 4 cut(s) 184, 1020, 1071, 1098
DpnII GATC 4 cut(s) 182, 1018, 1069, 1096
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 893
EarI CTCTTC 1 cut(s) 893
Eco57I CTGAAG 2 cut(s) 380, 944
Eco88I CYCGRG 1 cut(s) 147
EcoO109I RGGNCCY 1 cut(s) 250
EcoRI GAATTC 1 cut(s) 207
EcoRII CCWGG 3 cut(s) 646, 657, 1072
Esp3I CGTCTC 1 cut(s) 600
FaeI CATG 8 cut(s) 215, 220, 626, 876, 985, 1036, 1058, 1081
FalI AAGNNNNNCTT 2 cut(s) 842, 874
FaqI GGGAC 2 cut(s) 150, 777
FatI CATG 8 cut(s) 211, 216, 622, 872, 981, 1032, 1054, 1077
FblI GTMKAC 2 cut(s) 1087, 1147
Fnu4HI GCNGC 2 cut(s) 289, 484
FokI GGATG 3 cut(s) 455, 1024, 1139
Fsp4HI GCNGC 2 cut(s) 289, 484
FspBI CTAG 1 cut(s) 836
GlaI GCGC 2 cut(s) 603, 663
GluI GCNGC 2 cut(s) 289, 484
GsuI CTGGAG 1 cut(s) 28
HaeII RGCGCY 2 cut(s) 605, 665
HaeIII GGCC 2 cut(s) 5, 251
HapII CCGG 4 cut(s) 24, 110, 368, 795
HhaI GCGC 2 cut(s) 604, 664
Hin1II CATG 8 cut(s) 215, 220, 626, 876, 985, 1036, 1058, 1081
Hin6I GCGC 2 cut(s) 602, 662
HinP1I GCGC 2 cut(s) 602, 662
HinfI GANTC 5 cut(s) 143, 238, 513, 590, 1172
HpaII CCGG 4 cut(s) 24, 110, 368, 795
HphI GGTGA 3 cut(s) 662, 956, 958
Hpy166II GTNNAC 4 cut(s) 415, 562, 1088, 1148
Hpy188I TCNGA 4 cut(s) 206, 360, 670, 741
Hpy188III TCNNGA 7 cut(s) 147, 710, 806, 836, 982, 1100, 1111
Hpy8I GTNNAC 4 cut(s) 415, 562, 1088, 1148
HpyAV CCTTC 4 cut(s) 241, 497, 919, 979
HpyCH4III ACNGT 6 cut(s) 133, 412, 612, 702, 767, 1001
HpyCH4IV ACGT 1 cut(s) 30
HpyCH4V TGCA 8 cut(s) 36, 320, 398, 415, 626, 772, 872, 1015
HpyF10VI GCNNNNNNNGC 5 cut(s) 278, 404, 421, 670, 1012
HpyF3I CTNAG 3 cut(s) 467, 597, 1162
HpySE526I ACGT 1 cut(s) 30
Hsp92II CATG 8 cut(s) 215, 220, 626, 876, 985, 1036, 1058, 1081
HspAI GCGC 2 cut(s) 602, 662
Kzo9I GATC 4 cut(s) 182, 1018, 1069, 1096
Lsp1109I GCAGC 1 cut(s) 495
LweI GCATC 4 cut(s) 354, 820, 898, 1002
MaeI CTAG 1 cut(s) 836
MaeII ACGT 1 cut(s) 30
MaeIII GTNAC 3 cut(s) 26, 304, 520
MalI GATC 4 cut(s) 184, 1020, 1071, 1098
MboI GATC 4 cut(s) 182, 1018, 1069, 1096
MboII GAAGA 9 cut(s) 167, 373, 794, 850, 910, 917, 1007, 1050, 1111
MfeI CAATTG 1 cut(s) 954
MflI RGATCY 1 cut(s) 1069
MhlI GDGCHC 2 cut(s) 204, 417
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 207, 954
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 232
MmeI TCCRAC 1 cut(s) 567
MnlI CCTC 6 cut(s) 52, 52, 568, 735, 745, 974
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 1118
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 258, 381, 815
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 4 cut(s) 24, 110, 368, 795
MspR9I CCNGG 5 cut(s) 110, 648, 659, 795, 1074
MunI CAATTG 1 cut(s) 954
MvaI CCWGG 3 cut(s) 648, 659, 1074
MvnI CGCG 1 cut(s) 288
MwoI GCNNNNNNNGC 5 cut(s) 278, 404, 421, 670, 1012
NciI CCSGG 2 cut(s) 110, 795
NdeII GATC 4 cut(s) 182, 1018, 1069, 1096
NlaIII CATG 8 cut(s) 215, 220, 626, 876, 985, 1036, 1058, 1081
NspI RCATGY 2 cut(s) 876, 1081
NspV TTCGAA 1 cut(s) 1170
PaeI GCATGC 1 cut(s) 1081
PaeR7I CTCGAG 1 cut(s) 147
PagI TCATGA 1 cut(s) 981
PcsI WCGNNNNNNNCGW 1 cut(s) 242
PdmI GAANNNNTTC 1 cut(s) 1118
PfeI GAWTC 4 cut(s) 143, 513, 590, 1172
PkrI GCNGC 2 cut(s) 290, 485
PleI GAGTC 1 cut(s) 232
PpsI GAGTC 1 cut(s) 232
Psp6I CCWGG 3 cut(s) 646, 657, 1072
PspGI CCWGG 3 cut(s) 646, 657, 1072
PspPI GGNCC 1 cut(s) 250
PstI CTGCAG 2 cut(s) 322, 774
PstNI CAGNNNCTG 2 cut(s) 492, 778
PsuI RGATCY 1 cut(s) 1069
SaqAI TTAA 3 cut(s) 258, 381, 815
SatI GCNGC 2 cut(s) 289, 484
Sau3AI GATC 4 cut(s) 182, 1018, 1069, 1096
Sau96I GGNCC 1 cut(s) 250
SchI GAGTC 1 cut(s) 232
ScrFI CCNGG 5 cut(s) 110, 648, 659, 795, 1074
SduI GDGCHC 2 cut(s) 204, 417
SfaNI GCATC 4 cut(s) 354, 820, 898, 1002
SfcI CTRYAG 2 cut(s) 318, 770
Sfr274I CTCGAG 1 cut(s) 147
SfuI TTCGAA 1 cut(s) 1170
SlaI CTCGAG 1 cut(s) 147
SmlI CTYRAG 1 cut(s) 147
SmoI CTYRAG 1 cut(s) 147
SphI GCATGC 1 cut(s) 1081
Sse9I AATT 2 cut(s) 207, 954
SsiI CCGC 2 cut(s) 288, 969
SspMI CTAG 1 cut(s) 836
StyD4I CCNGG 5 cut(s) 108, 646, 657, 793, 1072
TaaI ACNGT 6 cut(s) 133, 412, 612, 702, 767, 1001
TaiI ACGT 1 cut(s) 33
TaqI TCGA 3 cut(s) 148, 181, 1170
TasI AATT 2 cut(s) 207, 954
TauI GCSGC 1 cut(s) 291
TfiI GAWTC 4 cut(s) 143, 513, 590, 1172
Tru1I TTAA 3 cut(s) 258, 381, 815
Tru9I TTAA 3 cut(s) 258, 381, 815
TscAI CASTG 3 cut(s) 138, 497, 633
TseI GCWGC 1 cut(s) 483
TspRI CASTG 3 cut(s) 138, 497, 633
VneI GTGCAC 1 cut(s) 413
XapI RAATTY 1 cut(s) 207
XbaI TCTAGA 1 cut(s) 835
XceI RCATGY 2 cut(s) 876, 1081
XhoI CTCGAG 1 cut(s) 147
XmiI GTMKAC 2 cut(s) 1087, 1147
XmnI GAANNNNTTC 1 cut(s) 1118
XspI CTAG 1 cut(s) 836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.