FvH4_4g35420

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001217
Physical Location & Seq
Reverse (-)
32743066 .. 32749935
6870 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g35420.t1

Sequence Viewer

Length: 4212 bp
ATGGCGGATGACGACGACAACGTTTCGTCAACTCTTCCCCATGTCGGCGGTTTCCGGCTGCGCTGGGACGTGTTCTTAAGCTTCAGAGGCGAGGACACCCGGGAAACGATCACCAAGAATCTCTACGAGGCGCTCCAACGACACGGCGTACGGGTTTTCCTAGACAACGACGGTTTGGACCGCGGGGACGACATCGGCCCGACTCTACTGGAAGCTATCGAGGACTCGGCGGCGGCCATCATCGTCCTGTCGCCGAGATATGCCGACTCAAGGTGGTGTCTCAAAGAGCTTGCTAAGATTTGCGAGGGCAGAAGGAGACTGATTCTGCCTCTGTTTTACAAAGTGGATCCCTCCGAAGTTCGGCGGCAAACCGGACCGTTTGAGGAGCACTTCAGACGACATGAGCAGGTGTTTGATAGCGCCATGGTCTCTGGATGGAGGACTGCCATGACCAGAGTAGGCGAGAAAGCTGGTTTTGTCTTCAACAGCTCAAGAAGAGAAGGGGAGTTAATTACAGATGAGGAAAAGAAGAAAGAAATTATAGATGAGGAAAAGAAGAAAGAAGCAGATTTGATTGAAAAATTAGTAAGACGGGTTTTGACAGAAATGAACAAAACTCCTATGGGTCTCATACCGTACCCAGTGGGACTTCATTCCCGAGTCGAAGAGGTGATGAAGCAATTAGATATCGGATCCAAAACCGTTCAAGTTATCGGAGTGCATGGCATGGGTGGCATTGGCAAAACAACCCTTGCCAAGGCTCTTTTCAATCGACTTGTTGGTTATTTTGAGCGTCACAGTTTCATCTCAAATGTCAGAGAAAGCTCAGCAAAGGGTCTAATAACTCTGCAAACCACACTTATTAGTGATCTTTCCAAAGGGAAGATGCAGGCCGAAATTAATCAGACTAGTGATGGTATTGCTGCCATCAAAGTTGTTGTGAATGAGAAGCGAGTTCTCGTTGTTCTGGATGATGTTGATAACATAGACCAACTAAGTGCTTTAGTTGGCAATGGACGATGGTTTTATGAAGGAAGCCGAATAATTGTTACAACAAGAGATAGAGAACTCCTACCTAATCATGGTGTCAATCATAAGTTGTATGAGGTCAGGGAGTTGGACGCGTCTGATGCTCTAGAACTTTTCAGTCACCACGCACTGGGAGTTAGAGAGAACCCAGCATCTACCTTTCTGAAACTATCTAAGCAGATTGTGGCTCTTACTGGTGGGCTACCGTTAGCTATTGAAGTATTCGGCTGTAGCCTCTATGATAAGAGGAGAGTAGAGGTATGGACAGACGCTCTGGAAAAACTGAAACGGATTCGTCCAGGGAATCTTCAGGATGTGCTGATGATAAGCTACAATGGGCTAGATGACCAAGAGAAGTGCATCTTTCTAGACATTGCATGTTTATTTGTTAAAATGAAGACCAAAAGAGAAGACGCAGTTGTCATATTCAAGGGCTGTGGTTTTAATGGTGAAATAGGACTTACTGTCCTCACAGCAAGATCACTCATCAAGATCGCTGAAGACACTACATTGTGGATGCACGATCAACTCAGAGACATGGGAAGAGAAATCGTTACAAAAGAAAACGATTCACATCCTGGCATGCGTAGTAGACTATGGGATCGCGATGAAATCATGAATGTTTTCGAGCATGATAAGGGGACACCATCCATACAAGGGATTGTCTTAGACTTTGAAATGAAAAGGATGGTGAGTGACCTAGGTGGTGACACAATTTCTTGGTACAACTTTCGGAGAAGCCCCAATTGTACCTCTGCATTGACATACCTAAAGGAAAGATACAAAGCGCATCTTAAAAGCCAAGCAGAGAAAAAGGAAGAGGTCAAGATTTCCTCTAAAGCTTTAGGAGCAATGGTCAACCTTAGGCTGCTCCAAATGAATAACGTGCATTTGGAAGGGAACTTTAAGTTCCTTCCTGCCGGAGTCAAGTGGTTACAATGGAAAGGTTGTCCTTTGAGATCTCTCCCTTCTGATTTTTTACCTCGGCAACTCGCTGTCCTTGATCTTTCAGACAGCAATATCACAAGTTTGTGGGGAGGACGCAGTATCATGCAATGTTTCACTTGTCTGACCTGCAGTGGAGACGAAAACAAGGTGGCAGAGAAATTGATGTTCTTAAATCTAAGATACTGCATTTACCTAACTGATATTCCTGATTTATCTGGGAATCGGGCACTACGGCAGCTTAATCTTGAGCACTGTATTTCATTGACTAGGCTTCATGGCTCGATTGGGAACTTGAACACATTAGTCCACTTGAACCTGCGAGAGTGTTCAAAGCTTGTTGAACTTCCAAGTGATGTTTCTGGCCTGAAAAAGCTCGAATACCTAATCCTCTCTGGTTGCACCCAGTTCCAAAGACTGCCAAACAACATGGAAAGCTTGGTGTCACTGAAAGAACTTCTCCTTGATGAAACTGCTATACAAAGTCTTCCCCAATCTATCTTCCGGCTTACAAAACTCGAAAAGCTCAGCTTGAACCGGTGCAGTGTATTAAAAGAGCTACCTGAGGAGATTGGAAGGCTGTATTCTTTGAAAGAAATATCCTTTAATGGATCTGGTTTGGAAAAACTACCTGATTCCATTGGCTCTTTGGCAAACCTTGAGAAATTAAGATTGTTCTGGTGTAAATCACTAAAAACTCTTCCCAATTCCATTGGGAACCTCAATTTCTTAATGGAATTTAGTACTTATGGTACTCCACTCACTGAACTGCCTGCGAACATTGGTTCATTGTCAAGCTTAAAGGACTTATCAGTTGGGCAGGGTCGATTTCTGAGCTCATTGCCTGATTCAGTTGGAAGATTGAGTTCTTTAGTTGTGCTAAAGATAGAACAGACATCTATCACAGACCTCCCGCAGGATATTGGTGCCTTGAAAACACTTGAGAAACTTGAGCTGAGGAACTGTGAATCTCTTAGATCATTACCAGAGTCAATTGGAGAGATGAGGGCACTAACTTCTATAATCATAACTGCAGCAAATATCACTGAATTGCCAGAATCCATAGGGTTGCTGGAAAATCTTACCATGTTAATGTTGAACAGATGTAAGCAGTTCCGCACGCTTCCAGCATCAATAGGACAGTTAAAATCGTTGCACCAATTGCAGATGAAGGAAACTGCAGTGACCGAACTACCTGATAGCTTTGGGATGCTTTCTAGTTTGATGGTTTTGAGCATGGGGAAGAAGCCTCAAGCTGGGGGACCTGCAGAAGAGAATTTTATACTTCCAGCTTCCTTCTCAAATCTGTCCTTGCTGTACGAACTGGATGCCCGAGCTTGTCACATATCTGGTGACATTTCTGACGATTTCGAGAACCTGTCATCACTTGAAACTTTGAATCTCAGTCGTAATAGTTTCTGCCACCTTCCAGCCAGCCTGAGTGGCATGTCCGTGCTCCAAGAACTTCTATTGCCTCATTGTAGAAAGCTAAAGTCTCTGCCTCCGCTTCCCTCAAGTTTGAAAAAGGTGGATATTGCAAACTGTATTGCATTAGAAAGTATCTGTGATGTTTCAAACCTGGAGAACTTGAGTGAACTGAACCTTACAAATTGTAAGAAAGTTGAGGATATACCCGGGCTGGAATGCTTGAATTCCTTGGTAAGGTTGTATATGAGTGGCTGCAAGGCTTGCTCATCAGCTGTTAAGAGAAGACTTGCGAAAAAGAGCTACCTGAGGAAAATACGCAATCTTAGCATGCCTGGAAGCAAGATACCAGACTGGTTTTCTCAAGAAATGGTTACATTCTCAAAACGAGGAAACCGCCCTCTCAAAAGTGTAATTCTATGTGTTGTGGTTTCTCTCAACCATCAAATACCTGATGACCCAAGAGAAGAACTCCCTGCTGTGGTAGATATTCAAGCGCAGATCCTCATACTGGATTCTCCTACTTACACTACAGCATTGATCTTATCGGGAGTACCCAATACAAATGATGATCAATTTCACTTGTGCCGGTATCCAATTGATCATCCTTTGGTGTCCCAGTTGAAAGATGGTTATAAGATACATGTAAAGAGGAGAGAGCCGCCATATGTGAAAGGGGTGGAGCTGAAGAAGTGGGGGCTATATTTGATTTACGAAGGCGATGATGATTATGAAGGAGATGAAGAATCATTAAATGAAAGCCAGCAATCCCTTTCAGAACAACTGGCAAATTTCTTCAGCACTTTCGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1404

Amino Acids

157.52

Weight (kDa)

6.38

Isoelectric Point (pI)

44.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 22 - 173 1.6e-38 TIR domain
TIR_2 PF13676 24 - 117 3.9e-17 TIR domain
NB-ARC PF00931 220 - 386 5.1e-24 NB-ARC domain
NACHT PF05729 237 - 385 9.1e-08 NACHT domain
WHD_ROQ1 PF23282 459 - 529 3.1e-14 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 726 - 838 5.3e-11 Leucine-rich repeat region
LRR_14 PF23598 834 - 908 1.9e-09 Leucine-rich repeat region
LRR_14 PF23598 909 - 981 5.4e-10 Leucine-rich repeat region
LRR_14 PF23598 1029 - 1256 8.7e-17 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4035
AarI CACCTGC 1 cut(s) 397
AasI GACNNNNNNGTC 1 cut(s) 1448
Acc36I ACCTGC 4 cut(s) 397, 2111, 2301, 3247
AccB1I GGYRCC 1 cut(s) 2900
AccB7I CCANNNNNTGG 1 cut(s) 1159
AccI GTMKAC 1 cut(s) 1621
AccII CGCG 3 cut(s) 183, 1124, 1635
AclI AACGTT 1 cut(s) 21
AclWI GGATC 7 cut(s) 341, 354, 687, 700, 1638, 2593, 3895
AcoI YGGCCR 1 cut(s) 234
AcsI RAATTY 4 cut(s) 2711, 3250, 3625, 4189
AcuI CTGAAG 6 cut(s) 67, 376, 1322, 1548, 4106, 4180
AfaI GTAC 8 cut(s) 150, 638, 1754, 1780, 2719, 2728, 3293, 3954
AflII CTTAAG 1 cut(s) 76
AflIII ACRYGT 3 cut(s) 69, 1122, 4042
AgeI ACCGGT 1 cut(s) 2511
AhdI GACNNNNNGTC 1 cut(s) 1493
AhlI ACTAGT 1 cut(s) 908
AjiI CACGTC 1 cut(s) 70
AjnI CCWGG 4 cut(s) 1327, 1606, 3552, 3733
AjuI GAANNNNNNNTTGG 4 cut(s) 749, 781, 2317, 2349
Alw21I GWGCWC 4 cut(s) 390, 2229, 2813, 3432
Alw26I GTCTC 7 cut(s) 284, 310, 433, 632, 1557, 2106, 3474
AlwI GGATC 7 cut(s) 341, 354, 687, 700, 1638, 2593, 3895
Ama87I CYCGRG 4 cut(s) 99, 657, 3306, 3608
AoxI GGCC 4 cut(s) 196, 234, 891, 2338
ApeKI GCWGC 6 cut(s) 58, 923, 1897, 2212, 3008, 3654
ApoI RAATTY 4 cut(s) 2711, 3250, 3625, 4189
AseI ATTAAT 1 cut(s) 900
AsiGI ACCGGT 1 cut(s) 2511
Asp700I GAANNNNTTC 1 cut(s) 1652
AspA2I CCTAGG 1 cut(s) 1729
AspLEI GCGC 5 cut(s) 63, 133, 422, 1819, 3898
AspS9I GGNCC 4 cut(s) 178, 197, 374, 3236
AsuC2I CCSGG 4 cut(s) 100, 101, 3609, 3610
AsuHPI GGTGA 7 cut(s) 103, 682, 1142, 1490, 1732, 1748, 3338
AsuII TTCGAA 1 cut(s) 4206
AvaI CYCGRG 4 cut(s) 99, 657, 3306, 3608
AvaII GGWCC 3 cut(s) 178, 374, 3236
AvrII CCTAGG 1 cut(s) 1729
AxyI CCTNAGG 3 cut(s) 1892, 2538, 3707
BaeGI GKGCMC 2 cut(s) 2206, 2986
BamHI GGATCC 2 cut(s) 346, 692
BanI GGYRCC 1 cut(s) 2900
BanII GRGCYC 1 cut(s) 2813
BbsI GAAGAC 6 cut(s) 472, 1433, 1446, 1536, 2453, 3691
Bbv12I GWGCWC 4 cut(s) 390, 2229, 2813, 3432
BbvCI CCTCAGC 1 cut(s) 2930
BbvI GCAGC 6 cut(s) 45, 910, 1884, 2224, 3020, 3641
BceAI ACGGC 2 cut(s) 160, 2225
BcgI CGANNNNNNTGC 2 cut(s) 3284, 3318
BciT130I CCWGG 4 cut(s) 1329, 1608, 3554, 3735
BciVI GTATCC 1 cut(s) 4002
BclI TGATCA 2 cut(s) 3970, 4000
BcnI CCSGG 4 cut(s) 100, 101, 3609, 3610
BcoDI GTCTC 7 cut(s) 284, 310, 433, 632, 1557, 2106, 3474
BcuI ACTAGT 1 cut(s) 908
BfaI CTAG 8 cut(s) 161, 909, 1136, 1370, 1397, 1730, 2244, 3192
BfmI CTRYAG 6 cut(s) 1258, 2104, 3006, 3153, 3240, 3930
BfoI RGCGCY 2 cut(s) 134, 423
BfrI CTTAAG 1 cut(s) 76
BfuAI ACCTGC 4 cut(s) 397, 2111, 2301, 3247
BfuI GTATCC 1 cut(s) 4002
BglI GCCNNNNNGGC 1 cut(s) 3417
BglII AGATCT 1 cut(s) 1988
BlnI CCTAGG 1 cut(s) 1729
BlpI GCTNAGC 2 cut(s) 826, 2501
BmcAI AGTACT 1 cut(s) 2719
Bme1390I CCNGG 8 cut(s) 100, 101, 1329, 1608, 3554, 3609, 3610, 3735
Bme18I GGWCC 3 cut(s) 178, 374, 3236
BmeRI GACNNNNNGTC 1 cut(s) 1493
BmeT110I CYCGRG 4 cut(s) 99, 657, 3306, 3608
BmgBI CACGTC 1 cut(s) 70
BmgT120I GGNCC 4 cut(s) 178, 197, 374, 3236
BmiI GGNNCC 5 cut(s) 348, 694, 2693, 2902, 3237
BmrFI CCNGG 8 cut(s) 100, 101, 1329, 1608, 3554, 3609, 3610, 3735
BmrI ACTGGG 4 cut(s) 635, 1169, 2374, 4012
BmsI GCATC 9 cut(s) 876, 1120, 1190, 1398, 1536, 1828, 3113, 3174, 3292
BmuI ACTGGG 4 cut(s) 635, 1169, 2374, 4012
BpiI GAAGAC 6 cut(s) 472, 1433, 1446, 1536, 2453, 3691
BplI GAGNNNNNCTC 2 cut(s) 3855, 3887
BpmI CTGGAG 1 cut(s) 3575
Bpu10I CCTNAGC 1 cut(s) 2930
Bpu1102I GCTNAGC 2 cut(s) 826, 2501
Bpu14I TTCGAA 1 cut(s) 4206
BpuMI CCSGG 4 cut(s) 100, 101, 3609, 3610
BsaBI GATNNNNATC 1 cut(s) 1602
BsaI GGTCTC 2 cut(s) 433, 632
BsaJI CCNNGG 9 cut(s) 99, 181, 423, 756, 1328, 1729, 2012, 3608, 3630
BsaWI WCCGGW 2 cut(s) 371, 2511
BsaXI ACNNNNNCTCC 2 cut(s) 1869, 1899
Bse118I RCCGGY 2 cut(s) 2511, 3987
Bse21I CCTNAGG 3 cut(s) 1892, 2538, 3707
Bse3DI GCAATG 5 cut(s) 1018, 1401, 1887, 2090, 2813
Bse8I GATNNNNATC 1 cut(s) 1602
BseBI CCWGG 4 cut(s) 1329, 1608, 3554, 3735
BseDI CCNNGG 9 cut(s) 99, 181, 423, 756, 1328, 1729, 2012, 3608, 3630
BseJI GATNNNNATC 1 cut(s) 1602
BseMI GCAATG 5 cut(s) 1018, 1401, 1887, 2090, 2813
BseMII CTCAG 9 cut(s) 840, 1573, 2515, 2529, 2798, 2921, 3391, 3404, 3698
BseRI GAGGAG 4 cut(s) 398, 1291, 2555, 4066
BseSI GKGCMC 2 cut(s) 2206, 2986
BseXI GCAGC 6 cut(s) 45, 910, 1884, 2224, 3020, 3641
BseYI CCCAGC 3 cut(s) 63, 1177, 3230
BsgI GTGCAG 1 cut(s) 2536
Bsh1236I CGCG 3 cut(s) 183, 1124, 1635
BshFI GGCC 4 cut(s) 198, 236, 893, 2340
BshNI GGYRCC 1 cut(s) 2900
BshTI ACCGGT 1 cut(s) 2511
BsiHKAI GWGCWC 4 cut(s) 390, 2229, 2813, 3432
BsiHKCI CYCGRG 4 cut(s) 99, 657, 3306, 3608
BsiSI CCGG 8 cut(s) 55, 100, 372, 1950, 2479, 2512, 3609, 3988
BsiWI CGTACG 1 cut(s) 148
BslFI GGGAC 6 cut(s) 80, 200, 660, 1684, 3249, 4000
BsmAI GTCTC 7 cut(s) 284, 310, 433, 632, 1557, 2106, 3474
BsmBI CGTCTC 1 cut(s) 2106
BsmFI GGGAC 6 cut(s) 80, 200, 660, 1684, 3249, 4000
BsmI GAATGC 1 cut(s) 3623
BsnI GGCC 4 cut(s) 198, 236, 893, 2340
Bso31I GGTCTC 2 cut(s) 433, 632
BsoBI CYCGRG 4 cut(s) 99, 657, 3306, 3608
Bsp119I TTCGAA 1 cut(s) 4206
Bsp1286I GDGCHC 6 cut(s) 390, 2206, 2229, 2813, 2986, 3432
Bsp1720I GCTNAGC 2 cut(s) 826, 2501
Bsp19I CCATGG 1 cut(s) 423
Bsp68I TCGCGA 1 cut(s) 1635
BspANI GGCC 4 cut(s) 198, 236, 893, 2340
BspCNI CTCAG 9 cut(s) 839, 1572, 2514, 2530, 2799, 2922, 3390, 3405, 3699
BspFNI CGCG 3 cut(s) 183, 1124, 1635
BspHI TCATGA 1 cut(s) 1644
BspLI GGNNCC 5 cut(s) 348, 694, 2693, 2902, 3237
BspMAI CTGCAG 4 cut(s) 2108, 3010, 3157, 3244
BspMI ACCTGC 4 cut(s) 397, 2111, 2301, 3247
BspPI GGATC 7 cut(s) 341, 354, 687, 700, 1638, 2593, 3895
BspT104I TTCGAA 1 cut(s) 4206
BspT107I GGYRCC 1 cut(s) 2900
BspTI CTTAAG 1 cut(s) 76
BspTNI GGTCTC 2 cut(s) 433, 632
BsrDI GCAATG 5 cut(s) 1018, 1401, 1887, 2090, 2813
BsrFI RCCGGY 2 cut(s) 2511, 3987
BssAI RCCGGY 2 cut(s) 2511, 3987
BssECI CCNNGG 9 cut(s) 99, 181, 423, 756, 1328, 1729, 2012, 3608, 3630
BssT1I CCWWGG 4 cut(s) 423, 756, 1729, 3630
Bst2UI CCWGG 4 cut(s) 1329, 1608, 3554, 3735
Bst6I CTCTTC 7 cut(s) 39, 490, 660, 1567, 1842, 2679, 3240
BstAFI CTTAAG 1 cut(s) 76
BstAPI GCANNNNNTGC 2 cut(s) 3136, 3663
BstBI TTCGAA 1 cut(s) 4206
BstC8I GCNNGC 9 cut(s) 291, 891, 1613, 2748, 3095, 3409, 3664, 3731, 4163
BstDSI CCRYGG 2 cut(s) 181, 423
BstENI CCTNNNNNAGG 3 cut(s) 755, 2888, 3634
BstFNI CGCG 3 cut(s) 183, 1124, 1635
BstH2I RGCGCY 2 cut(s) 134, 423
BstHHI GCGC 5 cut(s) 63, 133, 422, 1819, 3898
BstMAI GTCTC 7 cut(s) 284, 310, 433, 632, 1557, 2106, 3474
BstMWI GCNNNNNNNGC 8 cut(s) 87, 732, 1130, 1877, 3136, 3417, 3663, 3726
BstNI CCWGG 4 cut(s) 1329, 1608, 3554, 3735
BstNSI RCATGY 5 cut(s) 1410, 1615, 3424, 3733, 4046
BstSCI CCNGG 8 cut(s) 98, 99, 1327, 1606, 3552, 3607, 3608, 3733
BstSFI CTRYAG 6 cut(s) 1258, 2104, 3006, 3153, 3240, 3930
BstSLI GKGCMC 2 cut(s) 2206, 2986
BstUI CGCG 3 cut(s) 183, 1124, 1635
BstV1I GCAGC 6 cut(s) 45, 910, 1884, 2224, 3020, 3641
BstV2I GAAGAC 6 cut(s) 472, 1433, 1446, 1536, 2453, 3691
BstX2I RGATCY 5 cut(s) 346, 692, 1988, 2585, 3900
BstYI RGATCY 5 cut(s) 346, 692, 1988, 2585, 3900
Bsu36I CCTNAGG 3 cut(s) 1892, 2538, 3707
BsuI GTATCC 1 cut(s) 4002
BsuRI GGCC 4 cut(s) 198, 236, 893, 2340
BtgI CCRYGG 2 cut(s) 181, 423
BtgZI GCGATG 2 cut(s) 1650, 4134
BtrI CACGTC 1 cut(s) 70
BtsI GCAGTG 3 cut(s) 2113, 2524, 3162
BtsIMutI CAGTG 9 cut(s) 648, 1157, 2113, 2227, 2420, 2524, 2736, 3018, 3162
BtuMI TCGCGA 1 cut(s) 1635
BveI ACCTGC 4 cut(s) 397, 2111, 2301, 3247
Cac8I GCNNGC 9 cut(s) 291, 891, 1613, 2748, 3095, 3409, 3664, 3731, 4163
CciI TCATGA 1 cut(s) 1644
CfoI GCGC 5 cut(s) 63, 133, 422, 1819, 3898
Cfr10I RCCGGY 2 cut(s) 2511, 3987
Cfr13I GGNCC 4 cut(s) 178, 197, 374, 3236
Cfr42I CCGCGG 1 cut(s) 184
Cfr9I CCCGGG 2 cut(s) 99, 3608
CpoI CGGWCCG 1 cut(s) 374
CseI GACGC 6 cut(s) 782, 1113, 1130, 1307, 1451, 2079
Csp6I GTAC 8 cut(s) 149, 637, 1753, 1779, 2718, 2727, 3292, 3953
CspAI ACCGGT 1 cut(s) 2511
CspCI CAANNNNNGTGG 2 cut(s) 1447, 1482
CspI CGGWCCG 1 cut(s) 374
CviQI GTAC 8 cut(s) 149, 637, 1753, 1779, 2718, 2727, 3292, 3953
DrdI GACNNNNNNGTC 1 cut(s) 1448
DriI GACNNNNNGTC 1 cut(s) 1493
DseDI GACNNNNNNGTC 1 cut(s) 1448
EaeI YGGCCR 1 cut(s) 234
Eam1104I CTCTTC 7 cut(s) 39, 490, 660, 1567, 1842, 2679, 3240
Eam1105I GACNNNNNGTC 1 cut(s) 1493
EarI CTCTTC 7 cut(s) 39, 490, 660, 1567, 1842, 2679, 3240
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 2811
Eco130I CCWWGG 4 cut(s) 423, 756, 1729, 3630
Eco24I GRGCYC 1 cut(s) 2813
Eco31I GGTCTC 2 cut(s) 433, 632
Eco32I GATATC 1 cut(s) 688
Eco47I GGWCC 3 cut(s) 178, 374, 3236
Eco53kI GAGCTC 1 cut(s) 2811
Eco57I CTGAAG 6 cut(s) 67, 376, 1322, 1548, 4106, 4180
Eco81I CCTNAGG 3 cut(s) 1892, 2538, 3707
Eco88I CYCGRG 4 cut(s) 99, 657, 3306, 3608
EcoICRI GAGCTC 1 cut(s) 2811
EcoNI CCTNNNNNAGG 3 cut(s) 755, 2888, 3634
EcoO109I RGGNCCY 1 cut(s) 3236
EcoRI GAATTC 1 cut(s) 3625
EcoRII CCWGG 4 cut(s) 1327, 1606, 3552, 3733
EcoRV GATATC 1 cut(s) 688
EcoT14I CCWWGG 4 cut(s) 423, 756, 1729, 3630
EcoT38I GRGCYC 1 cut(s) 2813
ErhI CCWWGG 4 cut(s) 423, 756, 1729, 3630
Esp3I CGTCTC 1 cut(s) 2106
FalI AAGNNNNNCTT 6 cut(s) 1376, 1408, 1806, 1838, 2489, 2521
FaqI GGGAC 6 cut(s) 80, 200, 660, 1684, 3249, 4000
FauI CCCGC 2 cut(s) 176, 2895
FauNDI CATATG 1 cut(s) 4066
FbaI TGATCA 2 cut(s) 3970, 4000
FblI GTMKAC 1 cut(s) 1621
FriOI GRGCYC 1 cut(s) 2813
FspBI CTAG 8 cut(s) 161, 909, 1136, 1370, 1397, 1730, 2244, 3192
GlaI GCGC 5 cut(s) 62, 132, 421, 1818, 3897
GsaI CCCAGC 3 cut(s) 67, 1181, 3234
GsuI CTGGAG 1 cut(s) 3575
HaeII RGCGCY 2 cut(s) 134, 423
HaeIII GGCC 4 cut(s) 198, 236, 893, 2340
HapII CCGG 8 cut(s) 55, 100, 372, 1950, 2479, 2512, 3609, 3988
HgaI GACGC 6 cut(s) 782, 1113, 1130, 1307, 1451, 2079
HhaI GCGC 5 cut(s) 63, 133, 422, 1819, 3898
Hin6I GCGC 5 cut(s) 61, 131, 420, 1817, 3896
HinP1I GCGC 5 cut(s) 61, 131, 420, 1817, 3896
HincII GTYRAC 2 cut(s) 30, 1888
HindII GTYRAC 2 cut(s) 30, 1888
HindIII AAGCTT 5 cut(s) 79, 1869, 2309, 2410, 2770
HpaII CCGG 8 cut(s) 55, 100, 372, 1950, 2479, 2512, 3609, 3988
HphI GGTGA 7 cut(s) 103, 682, 1142, 1490, 1732, 1748, 3338
Hpy166II GTNNAC 5 cut(s) 30, 1622, 1888, 2284, 3569
Hpy8I GTNNAC 5 cut(s) 30, 1622, 1888, 2284, 3569
Hpy99I CGWCG 2 cut(s) 17, 173
HpyCH4IV ACGT 3 cut(s) 21, 69, 1914
HpyF10VI GCNNNNNNNGC 8 cut(s) 87, 732, 1130, 1877, 3136, 3417, 3663, 3726
HpySE526I ACGT 3 cut(s) 21, 69, 1914
HspAI GCGC 5 cut(s) 61, 131, 420, 1817, 3896
Ksp22I TGATCA 2 cut(s) 3970, 4000
KspI CCGCGG 1 cut(s) 184
LmnI GCTCC 6 cut(s) 138, 385, 1877, 1905, 3435, 4081
Lsp1109I GCAGC 6 cut(s) 45, 910, 1884, 2224, 3020, 3641
LweI GCATC 9 cut(s) 876, 1120, 1190, 1398, 1536, 1828, 3113, 3174, 3292
MaeI CTAG 8 cut(s) 161, 909, 1136, 1370, 1397, 1730, 2244, 3192
MaeII ACGT 3 cut(s) 21, 69, 1914
MfeI CAATTG 4 cut(s) 1774, 2967, 3134, 3996
MflI RGATCY 5 cut(s) 346, 692, 1988, 2585, 3900
MhlI GDGCHC 6 cut(s) 390, 2206, 2229, 2813, 2986, 3432
MluI ACGCGT 1 cut(s) 1122
MlyI GAGTC 6 cut(s) 196, 218, 260, 669, 1962, 2972
MmeI TCCRAC 3 cut(s) 160, 1098, 2809
MroXI GAANNNNTTC 1 cut(s) 1652
MslI CAYNNNNRTG 2 cut(s) 3065, 3425
MspA1I CMGCKG 2 cut(s) 183, 3674
MspCI CTTAAG 1 cut(s) 76
MspI CCGG 8 cut(s) 55, 100, 372, 1950, 2479, 2512, 3609, 3988
MspR9I CCNGG 8 cut(s) 100, 101, 1329, 1608, 3554, 3609, 3610, 3735
MunI CAATTG 4 cut(s) 1774, 2967, 3134, 3996
Mva1269I GAATGC 1 cut(s) 3623
MvaI CCWGG 4 cut(s) 1329, 1608, 3554, 3735
MvnI CGCG 3 cut(s) 183, 1124, 1635
MwoI GCNNNNNNNGC 8 cut(s) 87, 732, 1130, 1877, 3136, 3417, 3663, 3726
NciI CCSGG 4 cut(s) 100, 101, 3609, 3610
NcoI CCATGG 1 cut(s) 423
NdeI CATATG 1 cut(s) 4066
NlaIV GGNNCC 5 cut(s) 348, 694, 2693, 2902, 3237
NmeAIII GCCGAG 3 cut(s) 206, 279, 1993
NmuCI GTSAC 8 cut(s) 794, 1148, 1724, 1736, 2418, 3157, 3314, 3326
NruI TCGCGA 1 cut(s) 1635
NspI RCATGY 5 cut(s) 1410, 1615, 3424, 3733, 4046
NspV TTCGAA 1 cut(s) 4206
PaeI GCATGC 2 cut(s) 1615, 3733
PagI TCATGA 1 cut(s) 1644
PaqCI CACCTGC 1 cut(s) 397
PciI ACATGT 1 cut(s) 4042
PcsI WCGNNNNNNNCGW 1 cut(s) 18
PctI GAATGC 1 cut(s) 3623
PdmI GAANNNNTTC 1 cut(s) 1652
Pfl23II CGTACG 1 cut(s) 148
PflMI CCANNNNNTGG 1 cut(s) 1159
PinAI ACCGGT 1 cut(s) 2511
PleI GAGTC 6 cut(s) 196, 218, 260, 668, 1961, 2971
PpsI GAGTC 6 cut(s) 196, 218, 260, 668, 1961, 2971
PpuMI RGGWCCY 1 cut(s) 3236
PscI ACATGT 1 cut(s) 4042
PshBI ATTAAT 1 cut(s) 900
PsiI TTATAA 1 cut(s) 4035
Psp124BI GAGCTC 1 cut(s) 2813
Psp1406I AACGTT 1 cut(s) 21
Psp5II RGGWCCY 1 cut(s) 3236
Psp6I CCWGG 4 cut(s) 1327, 1606, 3552, 3733
PspFI CCCAGC 3 cut(s) 63, 1177, 3230
PspGI CCWGG 4 cut(s) 1327, 1606, 3552, 3733
PspLI CGTACG 1 cut(s) 148
PspN4I GGNNCC 5 cut(s) 348, 694, 2693, 2902, 3237
PspPI GGNCC 4 cut(s) 178, 197, 374, 3236
PspPPI RGGWCCY 1 cut(s) 3236
PstI CTGCAG 4 cut(s) 2108, 3010, 3157, 3244
PsuI RGATCY 5 cut(s) 346, 692, 1988, 2585, 3900
PvuII CAGCTG 1 cut(s) 3674
RruI TCGCGA 1 cut(s) 1635
RsaI GTAC 8 cut(s) 150, 638, 1754, 1780, 2719, 2728, 3293, 3954
RsaNI GTAC 8 cut(s) 149, 637, 1753, 1779, 2718, 2727, 3292, 3953
RseI CAYNNNNRTG 2 cut(s) 3065, 3425
Rsr2I CGGWCCG 1 cut(s) 374
RsrII CGGWCCG 1 cut(s) 374
SacI GAGCTC 1 cut(s) 2813
SacII CCGCGG 1 cut(s) 184
Sau96I GGNCC 4 cut(s) 178, 197, 374, 3236
ScaI AGTACT 1 cut(s) 2719
SchI GAGTC 6 cut(s) 196, 218, 260, 669, 1962, 2972
ScrFI CCNGG 8 cut(s) 100, 101, 1329, 1608, 3554, 3609, 3610, 3735
SduI GDGCHC 6 cut(s) 390, 2206, 2229, 2813, 2986, 3432
SfaNI GCATC 9 cut(s) 876, 1120, 1190, 1398, 1536, 1828, 3113, 3174, 3292
SfcI CTRYAG 6 cut(s) 1258, 2104, 3006, 3153, 3240, 3930
Sfr303I CCGCGG 1 cut(s) 184
SfuI TTCGAA 1 cut(s) 4206
SgrBI CCGCGG 1 cut(s) 184
SinI GGWCC 3 cut(s) 178, 374, 3236
SmaI CCCGGG 2 cut(s) 101, 3610
SmiMI CAYNNNNRTG 2 cut(s) 3065, 3425
SpeI ACTAGT 1 cut(s) 908
SphI GCATGC 2 cut(s) 1615, 3733
SspMI CTAG 8 cut(s) 161, 909, 1136, 1370, 1397, 1730, 2244, 3192
SstI GAGCTC 1 cut(s) 2813
StyD4I CCNGG 8 cut(s) 98, 99, 1327, 1606, 3552, 3607, 3608, 3733
StyI CCWWGG 4 cut(s) 423, 756, 1729, 3630
TaiI ACGT 3 cut(s) 24, 72, 1917
TaqII GACCGA 1 cut(s) 3176
TatI WGTACW 1 cut(s) 2717
TauI GCSGC 4 cut(s) 233, 236, 367, 4063
TscAI CASTG 9 cut(s) 648, 1164, 2113, 2234, 2427, 2524, 2743, 3025, 3162
TseFI GTSAC 8 cut(s) 794, 1148, 1724, 1736, 2418, 3157, 3314, 3326
TseI GCWGC 6 cut(s) 58, 923, 1897, 2212, 3008, 3654
Tsp45I GTSAC 8 cut(s) 794, 1148, 1724, 1736, 2418, 3157, 3314, 3326
TspGWI ACGGA 2 cut(s) 1333, 3415
TspMI CCCGGG 2 cut(s) 99, 3608
TspRI CASTG 9 cut(s) 648, 1164, 2113, 2234, 2427, 2524, 2743, 3025, 3162
Van91I CCANNNNNTGG 1 cut(s) 1159
Vha464I CTTAAG 1 cut(s) 76
VpaK11BI GGWCC 3 cut(s) 178, 374, 3236
VspI ATTAAT 1 cut(s) 900
XagI CCTNNNNNAGG 3 cut(s) 755, 2888, 3634
XapI RAATTY 4 cut(s) 2711, 3250, 3625, 4189
XbaI TCTAGA 2 cut(s) 1135, 1396
XceI RCATGY 5 cut(s) 1410, 1615, 3424, 3733, 4046
XcmI CCANNNNNNNNNTGG 3 cut(s) 2620, 3043, 4025
XmaI CCCGGG 2 cut(s) 99, 3608
XmaJI CCTAGG 1 cut(s) 1729
XmiI GTMKAC 1 cut(s) 1621
XmnI GAANNNNTTC 1 cut(s) 1652
XspI CTAG 8 cut(s) 161, 909, 1136, 1370, 1397, 1730, 2244, 3192
ZrmI AGTACT 1 cut(s) 2719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.