Rmu_co8224486.1_g000001

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8224486.1
OG0001217
Physical Location & Seq
Reverse (-)
171 .. 692
522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8224486.1_g000001.1.cds

Sequence Viewer

Length: 522 bp
atgctgcagagctacctgaggaaaatacgcaatcttagcatgcctggatgcaacatacccgactggttttctcaagaaatggttacattctcaaaacgagaaaaccgccccctcaaaagtgtaattctctgtgttgtagtctcgctcaaccatcaaacaccagatgacacaagagaagaatttcctgctgtggtagatattcaagcacagatcctcatactggattctcctactttcactacaacattggtcttatcgggagtaacaaatacaaatgaagatcaatttcacttgtgccggtatccaattgatcatcctttggtatccaaattgaaagatggttataaaatacacgtaatgaggagagagccgccatatatgacaggggtggagctgaagaagtgggggctttatttgatttacgaaggtgatgatgactatgaaggagatgaagaatcattaaatgaaagccagcaatccctttcagaacaactggcaaatttcttcagctcttttgaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

173

Amino Acids

20.01

Weight (kDa)

4.83

Isoelectric Point (pI)

47.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 345
AciI CCGC 2 cut(s) 106, 371
AclWI GGATC 1 cut(s) 205
AcsI RAATTY 2 cut(s) 179, 499
AcuI CTGAAG 2 cut(s) 416, 490
AfiI CCNNNNNNNGG 1 cut(s) 220
AflIII ACRYGT 1 cut(s) 352
AgsI TTSAA 3 cut(s) 203, 334, 518
AjnI CCWGG 1 cut(s) 43
AluBI AGCT 3 cut(s) 12, 394, 510
AluI AGCT 3 cut(s) 12, 394, 510
Alw26I GTCTC 1 cut(s) 145
AlwI GGATC 1 cut(s) 205
ApeKI GCWGC 1 cut(s) 4
ApoI RAATTY 2 cut(s) 179, 499
Asp700I GAANNNNTTC 1 cut(s) 180
AsuHPI GGTGA 1 cut(s) 440
AxyI CCTNAGG 1 cut(s) 17
BccI CCATC 2 cut(s) 159, 332
BciT130I CCWGG 1 cut(s) 45
BciVI GTATCC 2 cut(s) 312, 334
BclI TGATCA 1 cut(s) 310
BcoDI GTCTC 1 cut(s) 145
BfmI CTRYAG 1 cut(s) 5
BfuI GTATCC 2 cut(s) 312, 334
BisI GCNGC 2 cut(s) 5, 371
BlsI GCNGC 2 cut(s) 6, 372
Bme1390I CCNGG 1 cut(s) 45
BmrFI CCNGG 1 cut(s) 45
BmsI GCATC 1 cut(s) 38
BpuEI CTTGAG 1 cut(s) 57
BsaAI YACGTR 1 cut(s) 355
Bsc4I CCNNNNNNNGG 1 cut(s) 220
Bse118I RCCGGY 1 cut(s) 297
Bse1I ACTGG 3 cut(s) 68, 225, 498
Bse21I CCTNAGG 1 cut(s) 17
BseBI CCWGG 1 cut(s) 45
BseGI GGATG 2 cut(s) 53, 313
BseLI CCNNNNNNNGG 1 cut(s) 220
BseMII CTCAG 1 cut(s) 8
BseNI ACTGG 3 cut(s) 68, 225, 498
BseRI GAGGAG 1 cut(s) 376
BsiSI CCGG 1 cut(s) 298
BslI CCNNNNNNNGG 1 cut(s) 220
BsmAI GTCTC 1 cut(s) 145
Bsp143I GATC 3 cut(s) 210, 280, 310
BspACI CCGC 2 cut(s) 106, 371
BspCNI CTCAG 1 cut(s) 9
BspMAI CTGCAG 1 cut(s) 9
BspPI GGATC 1 cut(s) 205
BsrFI RCCGGY 1 cut(s) 297
BsrI ACTGG 3 cut(s) 68, 225, 498
BssAI RCCGGY 1 cut(s) 297
BssMI GATC 3 cut(s) 210, 280, 310
Bst2UI CCWGG 1 cut(s) 45
BstBAI YACGTR 1 cut(s) 355
BstC8I GCNNGC 2 cut(s) 41, 473
BstDEI CTNAG 2 cut(s) 17, 35
BstF5I GGATG 2 cut(s) 53, 313
BstKTI GATC 3 cut(s) 213, 283, 313
BstMAI GTCTC 1 cut(s) 145
BstMBI GATC 3 cut(s) 210, 280, 310
BstMWI GCNNNNNNNGC 1 cut(s) 36
BstNI CCWGG 1 cut(s) 45
BstNSI RCATGY 1 cut(s) 43
BstSCI CCNGG 1 cut(s) 43
BstSFI CTRYAG 1 cut(s) 5
BstX2I RGATCY 1 cut(s) 210
BstYI RGATCY 1 cut(s) 210
Bsu36I CCTNAGG 1 cut(s) 17
BsuI GTATCC 2 cut(s) 312, 334
BtsCI GGATG 2 cut(s) 53, 313
Cac8I GCNNGC 2 cut(s) 41, 473
Cfr10I RCCGGY 1 cut(s) 297
CviAII CATG 1 cut(s) 40
CviJI RGCY 6 cut(s) 12, 370, 394, 409, 471, 510
CviKI_1 RGCY 6 cut(s) 12, 370, 394, 409, 471, 510
DdeI CTNAG 2 cut(s) 17, 35
DpnI GATC 3 cut(s) 212, 282, 312
DpnII GATC 3 cut(s) 210, 280, 310
Eco57I CTGAAG 2 cut(s) 416, 490
Eco81I CCTNAGG 1 cut(s) 17
EcoRII CCWGG 1 cut(s) 43
FaeI CATG 1 cut(s) 43
FaiI YATR 8 cut(s) 41, 56, 218, 345, 376, 378, 380, 441
FatI CATG 1 cut(s) 39
FbaI TGATCA 1 cut(s) 310
Fnu4HI GCNGC 2 cut(s) 5, 371
FokI GGATG 2 cut(s) 60, 300
Fsp4HI GCNGC 2 cut(s) 5, 371
GluI GCNGC 2 cut(s) 5, 371
HapII CCGG 1 cut(s) 298
Hin1II CATG 1 cut(s) 43
HinfI GANTC 2 cut(s) 224, 455
HpaII CCGG 1 cut(s) 298
HphI GGTGA 1 cut(s) 440
Hpy188I TCNGA 1 cut(s) 487
Hpy188III TCNNGA 2 cut(s) 74, 258
HpyAV CCTTC 2 cut(s) 419, 437
HpyCH4IV ACGT 1 cut(s) 354
HpyCH4V TGCA 2 cut(s) 7, 51
HpyF10VI GCNNNNNNNGC 1 cut(s) 36
HpyF3I CTNAG 2 cut(s) 17, 35
HpySE526I ACGT 1 cut(s) 354
Hsp92II CATG 1 cut(s) 43
Ksp22I TGATCA 1 cut(s) 310
Kzo9I GATC 3 cut(s) 210, 280, 310
LmnI GCTCC 1 cut(s) 391
LweI GCATC 1 cut(s) 38
MaeII ACGT 1 cut(s) 354
MaeIII GTNAC 2 cut(s) 82, 262
MalI GATC 3 cut(s) 212, 282, 312
MboI GATC 3 cut(s) 210, 280, 310
MboII GAAGA 5 cut(s) 188, 290, 409, 464, 496
MfeI CAATTG 1 cut(s) 306
MflI RGATCY 1 cut(s) 210
MluCI AATT 6 cut(s) 123, 179, 284, 306, 329, 499
MnlI CCTC 4 cut(s) 12, 122, 224, 354
MroXI GAANNNNTTC 1 cut(s) 180
MseI TTAA 1 cut(s) 461
MspI CCGG 1 cut(s) 298
MspR9I CCNGG 1 cut(s) 45
MunI CAATTG 1 cut(s) 306
MvaI CCWGG 1 cut(s) 45
MwoI GCNNNNNNNGC 1 cut(s) 36
NdeII GATC 3 cut(s) 210, 280, 310
NlaIII CATG 1 cut(s) 43
NspI RCATGY 1 cut(s) 43
PaeI GCATGC 1 cut(s) 43
PdmI GAANNNNTTC 1 cut(s) 180
PfeI GAWTC 2 cut(s) 224, 455
PkrI GCNGC 2 cut(s) 6, 372
Ppu21I YACGTR 1 cut(s) 355
PsiI TTATAA 1 cut(s) 345
Psp6I CCWGG 1 cut(s) 43
PspGI CCWGG 1 cut(s) 43
PstI CTGCAG 1 cut(s) 9
PsuI RGATCY 1 cut(s) 210
SaqAI TTAA 1 cut(s) 461
SatI GCNGC 2 cut(s) 5, 371
Sau3AI GATC 3 cut(s) 210, 280, 310
ScrFI CCNGG 1 cut(s) 45
SetI ASST 6 cut(s) 14, 18, 357, 396, 430, 512
SfaNI GCATC 1 cut(s) 38
SfcI CTRYAG 1 cut(s) 5
SmlI CTYRAG 1 cut(s) 72
SmoI CTYRAG 1 cut(s) 72
SphI GCATGC 1 cut(s) 43
Sse9I AATT 6 cut(s) 123, 179, 284, 306, 329, 499
SsiI CCGC 2 cut(s) 106, 371
StyD4I CCNGG 1 cut(s) 43
TaiI ACGT 1 cut(s) 357
TasI AATT 6 cut(s) 123, 179, 284, 306, 329, 499
TauI GCSGC 1 cut(s) 373
TfiI GAWTC 2 cut(s) 224, 455
Tru1I TTAA 1 cut(s) 461
Tru9I TTAA 1 cut(s) 461
TseI GCWGC 1 cut(s) 4
TspDTI ATGAA 4 cut(s) 291, 456, 465, 480
XapI RAATTY 2 cut(s) 179, 499
XceI RCATGY 1 cut(s) 43
XmnI GAANNNNTTC 1 cut(s) 180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.