Rroxscaffold_5G00385590

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001217
Physical Location & Seq
Forward (+)
64627623 .. 64633756
6134 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00385590.1

Sequence Viewer

Length: 3126 bp
ATGCCGTACGACGGCGCAGTGGGCGGTTTCCGGCTCCGGTGGGACGTATTCCTAAGCTTTAGAGGAGAAGACACCCGCCACACCATCACCAACAGCCTCTACAACGCGCTCGAACGACGCGGCGTACGAGTCTTCCGCGACGACGACGGCCTCAACCGCGGCGACGACATCGCGCCGAGTCTTCTGGAAGCGATCGAGGACTCGGCGGCTGCCGTGGTCGTGTTGTCGCCGAGATACGCCGACTCGAGGTGGTGCCTTGAGGAGCTCGCGAAGATCTGCGACGGCGGCCGGAGACTGATCCTGCCGGTGTTTTACCAAGTGGACCCCTCCGACGTCCGGCGACAGAGCGGACCGTTTGAAAAGGACTTCATATCTCATGAAGAGCGTTTTGATAGCGACAAGGTCTCTAAATGGAGAAACGCCATGGCCAAAGTAGGTGGCAAAGCTGGACCTAAGGGCTTGGTATCTCTTCAAAACAACCTCATCGCCGATCTTTCCCCTGGTAAGGTGCCTGTGAATGACCTTGAGACTGGTATTTCTGCGATTAAGGCACTTGTATATGATAAGCGAGTTCTTGTTGTTCTGGATGATGTTGATAATGTAAACCAACTAAGTGCTTTAGTTGGCAAAGGACAATGGTTTAATGAAGGAAGCCGAATTATCATTACCACACGAGATAGAGGACTATTAGCCAGTTACCTTGTGAATTTTAAGTTGTATGAGGTCAGGGAGTTGGATGTGTCCCAGGCACTACAACTGTTTATTTACCATGCACTGAGAAGAGATAAACCCACAGATAATTTGTTGAATCTGTCCAAGGAAATTGTATCTCTTACAGGTGGGCTACCGTTAGCTCTTGAAGTATTTGGTTCTTTTCTGTTTGATAAGAGGAGAATAGAGGAATGGACAGATGCTCTGCACAAACTGAATAAGATTCGACCACAACATCTTCAGGATGTGTTGAAGATCAGTTATGATGCACTAGACGAGCAAGAGAAGTGTATCTTCTTAGACATTGCATGCTTAATTGTAAAAATGAATGCCAAAAGAGAAGATGCAATTGACATATTGAGGAGTTGCGGTTTTGATGGTGAGATAGCAATTGCTGACCTCACAGCAAAATCACTTATTAAGATTACAGAAGACACTACATTGTGGATGCACGATCAAGTTAGAGACATGGGAAGACAAATCGTGATACACGAAAGCCTTTTGGATCCTGGAATGCGTAGCAGACTGTGGGAACATGATAAAATCATGAATGTCTTCAAGGATGATACGGGGACAAGATGTATACAAGGCATAGTCCTAGACGACCTAGACACTGAAAGGAAAATGAAGGGGCCAAGGGATCTAACCGGTGACAAAATTTCTTGGAACAGCTTTCAAAGAAGCCCCAATTTTACCTCTGCAGTTACGTACTTAAAGGAAAGATACAAAGTATATCTTCAAAATCAAGCAGAGAAAAAGAGTCGGGTTACAATTCCCTCTAAACCCTTTGGAGCTATGGCCAATCTTAGACTGCTTCAGATGAATTATGTGAATTTGGAAGGGAGGTTCAAATTTCTTCCATCTGAATTGAAGTGGTTACAATGGAAAGGTTGTCCTCTGAAATCTCTTCCTTCTGTTCTTTTCCTTCGGCGACTTGCTGGCCTTGATCTTTCAGACAGTAGAGTGGAAAGATTGTATAGTGGTCACACAAATAAGGTGGCTGAGAAATTGATGTTCCTGAATCTAAGCGGCTGCAGTTTTCTCACTGCTATTCCTGATTTATCTGGGAATCATGCCTTGGAAAAGCTTATTCTTAAGAATTGCGTTGGACTGATCAAGCTCCATGATTCGATTGGGAATTTGAATACATTAATCCACTTGAACTTGCAAGACTGTACAAACCTCGTTGAACTTCCAAGTGATTTCTCCGGCCTGAGAAAACTTGAGAACCTAATCGTCACCGGTTGCTCAAAATTGAAAAGGCTGCCAAAAAACATCGGAAGCTTGGTATCACTAAAAAAACTTCTCCTTGATGAAACTGCTATTGAAAGTCTTCCTGAAGATATATTCCATCTTACAAAACTTGAAAAGCTGAGCTTGAACCGGTGCAGAGTTTTGAAAGGGCTACCTGGGGAGATTGGTAAGCTGTGTTCTTTGAAAGAAATCTCCCTTAATGGATCTGGTTTGGAAAAACTACCTTATGCCATTGGCTCTTTGGCAAACCTTGAGATACTACAGTTGATGTGGTGTCAGTCACTGACCACTATTCCTAACTCCCTTGGTGACCTTAATAACTTGATGGAATTTTTTACTTACGGTACTCCAATTGAAGAACTGCCGTTGAGTTTTGGTTCATTATCAAACTTGAAGGAATTATCTGTGGGACATGGCCAATTTCTGCAAACACTACCAGATTCCATTGGAGGACTAAAATCATTAGTTGTGTTAAAGATAGATGAGACATCAATCACGGGTCTTCCACAAGAGATTAGTGCCTTGAAAACACTTGAGAAACTTGAGCTGAGGAAATGTGAATCTCTTAGATCATTACCAGAGTCAATTGGAAGCATGAACCCGAGTGGTCTGTCCACTCTCCGAAAACTTTCATTGCCTCGCCGTAAGAAGCTCACGTCTTTGCCTCCACTTCCCTCAAGTTTGAAAGAGGTAGATGTTGCAAACCGTGTTGCATTAGAAAGTATATCCGATGTTTCAAACCTGGAGAACTTGACAGATTTCAACCTTACAAATTGTGAGAAAGTGTTAGATATTCCCGGCATTGAATGCTTGGATTCCTTGACAAGGTTTGTTTATGAGTGGTGCAACGAGCTTGCTCATCACCGTCAAGAGAAGACTAGCAAAAAAGATGGTTATAAGATACATGTAGAGAGGAGAGAGCCGCCGTATGTGAAAGGAGTGGAGCTGAAGAAGTGGGGGATTCATTTGGTGTATGAAGGTGATGATGATTACGAGGAAGACGAAGAGTCATTGAAGGAAAGCCGGCAGTCCCTTTCGGAAAAACTGGCCAAGTTCTTCACTCTTTTGATGAAGAAGCCGACTCTACCTCCACTCATGAGCAAAATGATTTCACCTCTGAATCTAGTTATAATGAAGATGAAGGAATGGCACAGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1041

Amino Acids

117.58

Weight (kDa)

7.75

Isoelectric Point (pI)

43.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 14 - 150 1.9e-38 TIR domain
TIR_2 PF13676 16 - 110 6.8e-17 TIR domain
WHD_ROQ1 PF23282 330 - 401 3.1e-13 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 589 - 672 1.4e-06 Leucine-rich repeat region
LRR_14 PF23598 656 - 744 7e-11 Leucine-rich repeat region
LRR_14 PF23598 709 - 791 8.3e-09 Leucine-rich repeat region
LRR_14 PF23598 733 - 814 1.6e-07 Leucine-rich repeat region
LRR_14 PF23598 804 - 930 3.7e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2862, 3095
AatII GACGTC 1 cut(s) 336
AccB1I GGYRCC 2 cut(s) 252, 508
AccBSI CCGCTC 1 cut(s) 348
AccI GTMKAC 1 cut(s) 1294
AccII CGCG 6 cut(s) 107, 120, 138, 159, 173, 269
AclWI GGATC 5 cut(s) 292, 1211, 1224, 1359, 2176
AcoI YGGCCR 5 cut(s) 286, 426, 1509, 2380, 3012
AcsI RAATTY 6 cut(s) 706, 1368, 1543, 1562, 1849, 2294
AcuI CTGAAG 4 cut(s) 935, 1511, 2070, 2933
AcyI GRCGYC 1 cut(s) 333
AfaI GTAC 5 cut(s) 8, 126, 1421, 1888, 2311
AfiI CCNNNNNNNGG 5 cut(s) 11, 246, 336, 505, 2790
AflII CTTAAG 1 cut(s) 1805
AflIII ACRYGT 1 cut(s) 2869
AgeI ACCGGT 3 cut(s) 1358, 1952, 2094
AhdI GACNNNNNGTC 1 cut(s) 2971
AjiI CACGTC 1 cut(s) 2622
AjnI CCWGG 5 cut(s) 499, 744, 1219, 2119, 2706
AjuI GAANNNNNNNTTGG 2 cut(s) 1772, 1804
AloI GAACNNNNNNTCC 2 cut(s) 1541, 1573
Alw21I GWGCWC 1 cut(s) 267
Alw26I GTCTC 5 cut(s) 286, 409, 521, 1170, 2444
AlwI GGATC 5 cut(s) 292, 1211, 1224, 1359, 2176
AlwNI CAGNNNCTG 1 cut(s) 2248
Ama87I CYCGRG 2 cut(s) 244, 2566
AoxI GGCC 9 cut(s) 148, 286, 426, 1343, 1509, 1651, 1921, 2380, 3012
ApeKI GCWGC 3 cut(s) 209, 1743, 1975
ApoI RAATTY 6 cut(s) 706, 1368, 1543, 1562, 1849, 2294
AseI ATTAAT 1 cut(s) 1862
AsiGI ACCGGT 3 cut(s) 1358, 1952, 2094
Asp700I GAANNNNTTC 3 cut(s) 1265, 2043, 2593
AspLEI GCGC 3 cut(s) 17, 109, 175
AspS9I GGNCC 4 cut(s) 322, 350, 449, 1343
AsuC2I CCSGG 1 cut(s) 2763
AsuHPI GGTGA 8 cut(s) 79, 1103, 1373, 1942, 2285, 2819, 2957, 3069
AvaI CYCGRG 2 cut(s) 244, 2566
AvaII GGWCC 3 cut(s) 322, 350, 449
AxyI CCTNAGG 1 cut(s) 453
BaeI ACNNNNGTAYC 2 cut(s) 2213, 2246
BalI TGGCCA 4 cut(s) 428, 1511, 2382, 3014
BamHI GGATCC 1 cut(s) 1216
BanI GGYRCC 2 cut(s) 252, 508
BanII GRGCYC 1 cut(s) 267
BauI CACGAG 1 cut(s) 672
Bbv12I GWGCWC 1 cut(s) 267
BbvCI CCTCAGC 1 cut(s) 2513
BbvI GCAGC 3 cut(s) 196, 1730, 1962
BccI CCATC 6 cut(s) 92, 1082, 1579, 2070, 2284, 2849
BceAI ACGGC 7 cut(s) 28, 163, 197, 298, 2314, 2592, 2875
BciT130I CCWGG 5 cut(s) 501, 746, 1221, 2121, 2708
BclI TGATCA 1 cut(s) 1824
BcnI CCSGG 1 cut(s) 2763
BcoDI GTCTC 5 cut(s) 286, 409, 521, 1170, 2444
BfaI CTAG 5 cut(s) 983, 1310, 1319, 2844, 3089
BfmI CTRYAG 3 cut(s) 1410, 1744, 2225
BfrI CTTAAG 1 cut(s) 1805
BglII AGATCT 1 cut(s) 273
BisI GCNGC 9 cut(s) 121, 160, 207, 210, 286, 1741, 1744, 1976, 2888
BlpI GCTNAGC 1 cut(s) 2084
BlsI GCNGC 9 cut(s) 122, 161, 208, 211, 287, 1742, 1745, 1977, 2889
Bme1390I CCNGG 6 cut(s) 501, 746, 1221, 2121, 2708, 2763
Bme18I GGWCC 3 cut(s) 322, 350, 449
BmeRI GACNNNNNGTC 1 cut(s) 2971
BmeT110I CYCGRG 2 cut(s) 244, 2566
BmgBI CACGTC 1 cut(s) 2622
BmgT120I GGNCC 4 cut(s) 322, 350, 449, 1343
BmiI GGNNCC 6 cut(s) 35, 254, 324, 510, 1218, 1344
BmrFI CCNGG 6 cut(s) 501, 746, 1221, 2121, 2708, 2763
BmsI GCATC 4 cut(s) 901, 967, 1045, 1149
BpmI CTGGAG 1 cut(s) 2729
Bpu10I CCTNAGC 2 cut(s) 53, 2513
Bpu1102I GCTNAGC 1 cut(s) 2084
BpuEI CTTGAG 7 cut(s) 278, 545, 1955, 2237, 2519, 2528, 2626
BpuMI CCSGG 1 cut(s) 2763
BsaAI YACGTR 1 cut(s) 1419
BsaHI GRCGYC 1 cut(s) 333
BsaI GGTCTC 1 cut(s) 409
BsaWI WCCGGW 4 cut(s) 36, 1358, 1952, 2094
BsaXI ACNNNNNCTCC 4 cut(s) 1066, 1096, 3037, 3067
Bsc4I CCNNNNNNNGG 5 cut(s) 11, 246, 336, 505, 2790
Bse118I RCCGGY 5 cut(s) 304, 1358, 1952, 2094, 2988
Bse1I ACTGG 3 cut(s) 535, 693, 3015
Bse21I CCTNAGG 1 cut(s) 453
Bse3DI GCAATG 2 cut(s) 1014, 2597
BseBI CCWGG 5 cut(s) 501, 746, 1221, 2121, 2708
BseGI GGATG 5 cut(s) 592, 742, 961, 1164, 1279
BseLI CCNNNNNNNGG 5 cut(s) 11, 246, 336, 505, 2790
BseMI GCAATG 2 cut(s) 1014, 2597
BseMII CTCAG 5 cut(s) 767, 1704, 1916, 2075, 2504
BseNI ACTGG 3 cut(s) 535, 693, 3015
BseRI GAGGAG 5 cut(s) 78, 275, 904, 1087, 2893
BseX3I CGGCCG 1 cut(s) 286
BseXI GCAGC 3 cut(s) 196, 1730, 1962
BsgI GTGCAG 2 cut(s) 902, 2119
Bsh1236I CGCG 6 cut(s) 107, 120, 138, 159, 173, 269
Bsh1285I CGRYCG 2 cut(s) 195, 289
BshFI GGCC 9 cut(s) 150, 288, 428, 1345, 1511, 1653, 1923, 2382, 3014
BshNI GGYRCC 2 cut(s) 252, 508
BshTI ACCGGT 3 cut(s) 1358, 1952, 2094
BsiEI CGRYCG 2 cut(s) 195, 289
BsiHKAI GWGCWC 1 cut(s) 267
BsiHKCI CYCGRG 2 cut(s) 244, 2566
BsiWI CGTACG 2 cut(s) 6, 124
BslFI GGGAC 5 cut(s) 56, 727, 1297, 2388, 2980
BslI CCNNNNNNNGG 5 cut(s) 11, 246, 336, 505, 2790
BsmAI GTCTC 5 cut(s) 286, 409, 521, 1170, 2444
BsmFI GGGAC 5 cut(s) 56, 727, 1297, 2388, 2980
BsmI GAATGC 3 cut(s) 1045, 1230, 2777
BsnI GGCC 9 cut(s) 150, 288, 428, 1345, 1511, 1653, 1923, 2382, 3014
Bso31I GGTCTC 1 cut(s) 409
BsoBI CYCGRG 2 cut(s) 244, 2566
Bsp1286I GDGCHC 1 cut(s) 267
Bsp1407I TGTACA 1 cut(s) 1886
Bsp1720I GCTNAGC 1 cut(s) 2084
Bsp19I CCATGG 1 cut(s) 423
Bsp68I TCGCGA 1 cut(s) 269
BspANI GGCC 9 cut(s) 150, 288, 428, 1345, 1511, 1653, 1923, 2382, 3014
BspCNI CTCAG 5 cut(s) 768, 1705, 1917, 2076, 2505
BspFNI CGCG 6 cut(s) 107, 120, 138, 159, 173, 269
BspHI TCATGA 3 cut(s) 376, 1257, 3060
BspLI GGNNCC 6 cut(s) 35, 254, 324, 510, 1218, 1344
BspMAI CTGCAG 2 cut(s) 1414, 1748
BspPI GGATC 5 cut(s) 292, 1211, 1224, 1359, 2176
BspQI GCTCTTC 1 cut(s) 375
BspT107I GGYRCC 2 cut(s) 252, 508
BspTI CTTAAG 1 cut(s) 1805
BspTNI GGTCTC 1 cut(s) 409
BsrBI CCGCTC 1 cut(s) 348
BsrDI GCAATG 2 cut(s) 1014, 2597
BsrFI RCCGGY 5 cut(s) 304, 1358, 1952, 2094, 2988
BsrGI TGTACA 1 cut(s) 1886
BsrI ACTGG 3 cut(s) 535, 693, 3015
BssAI RCCGGY 5 cut(s) 304, 1358, 1952, 2094, 2988
BssNAI GTATAC 1 cut(s) 1295
BssNI GRCGYC 1 cut(s) 333
BssSI CACGAG 1 cut(s) 672
BssT1I CCWWGG 5 cut(s) 423, 816, 1346, 1788, 2269
Bst1107I GTATAC 1 cut(s) 1295
Bst2BI CACGAG 1 cut(s) 672
Bst2UI CCWGG 5 cut(s) 501, 746, 1221, 2121, 2708
Bst6I CTCTTC 5 cut(s) 375, 474, 775, 1623, 2964
BstACI GRCGYC 1 cut(s) 333
BstAFI CTTAAG 1 cut(s) 1805
BstAPI GCANNNNNTGC 1 cut(s) 2772
BstAUI TGTACA 1 cut(s) 1886
BstBAI YACGTR 1 cut(s) 1419
BstC8I GCNNGC 5 cut(s) 267, 1021, 1651, 2820, 2990
BstDSI CCRYGG 3 cut(s) 157, 213, 423
BstEII GGTNACC 1 cut(s) 2273
BstENI CCTNNNNNAGG 1 cut(s) 2788
BstF5I GGATG 5 cut(s) 592, 742, 961, 1164, 1279
BstFNI CGCG 6 cut(s) 107, 120, 138, 159, 173, 269
BstHHI GCGC 3 cut(s) 17, 109, 175
BstMAI GTCTC 5 cut(s) 286, 409, 521, 1170, 2444
BstMCI CGRYCG 2 cut(s) 195, 289
BstMWI GCNNNNNNNGC 5 cut(s) 21, 156, 285, 548, 2772
BstNI CCWGG 5 cut(s) 501, 746, 1221, 2121, 2708
BstNSI RCATGY 2 cut(s) 1023, 2873
BstPI GGTNACC 1 cut(s) 2273
BstSCI CCNGG 6 cut(s) 499, 744, 1219, 2119, 2706, 2761
BstSFI CTRYAG 3 cut(s) 1410, 1744, 2225
BstSNI TACGTA 1 cut(s) 1419
BstUI CGCG 6 cut(s) 107, 120, 138, 159, 173, 269
BstV1I GCAGC 3 cut(s) 196, 1730, 1962
BstX2I RGATCY 4 cut(s) 273, 1216, 1351, 2168
BstYI RGATCY 4 cut(s) 273, 1216, 1351, 2168
BstZ17I GTATAC 1 cut(s) 1295
BstZI CGGCCG 1 cut(s) 286
Bsu36I CCTNAGG 1 cut(s) 453
BsuRI GGCC 9 cut(s) 150, 288, 428, 1345, 1511, 1653, 1923, 2382, 3014
BtgI CCRYGG 3 cut(s) 157, 213, 423
BtgZI GCGATG 2 cut(s) 154, 469
BtrI CACGTC 1 cut(s) 2622
BtsCI GGATG 5 cut(s) 592, 742, 961, 1164, 1279
BtsI GCAGTG 2 cut(s) 24, 1755
BtsIMutI CAGTG 5 cut(s) 24, 773, 1323, 1755, 2246
BtuMI TCGCGA 1 cut(s) 269
Cac8I GCNNGC 5 cut(s) 267, 1021, 1651, 2820, 2990
CaiI CAGNNNCTG 1 cut(s) 2248
CciI TCATGA 3 cut(s) 376, 1257, 3060
CfoI GCGC 3 cut(s) 17, 109, 175
Cfr10I RCCGGY 5 cut(s) 304, 1358, 1952, 2094, 2988
Cfr13I GGNCC 4 cut(s) 322, 350, 449, 1343
Cfr42I CCGCGG 1 cut(s) 160
CpoI CGGWCCG 1 cut(s) 350
CseI GACGC 1 cut(s) 126
Csp6I GTAC 5 cut(s) 7, 125, 1420, 1887, 2310
CspAI ACCGGT 3 cut(s) 1358, 1952, 2094
CspCI CAANNNNNGTGG 4 cut(s) 418, 453, 1689, 1724
CspI CGGWCCG 1 cut(s) 350
CviQI GTAC 5 cut(s) 7, 125, 1420, 1887, 2310
DriI GACNNNNNGTC 1 cut(s) 2971
EaeI YGGCCR 5 cut(s) 286, 426, 1509, 2380, 3012
EagI CGGCCG 1 cut(s) 286
Eam1104I CTCTTC 5 cut(s) 375, 474, 775, 1623, 2964
Eam1105I GACNNNNNGTC 1 cut(s) 2971
EarI CTCTTC 5 cut(s) 375, 474, 775, 1623, 2964
Ecl136II GAGCTC 1 cut(s) 265
EclXI CGGCCG 1 cut(s) 286
Eco105I TACGTA 1 cut(s) 1419
Eco130I CCWWGG 5 cut(s) 423, 816, 1346, 1788, 2269
Eco24I GRGCYC 1 cut(s) 267
Eco31I GGTCTC 1 cut(s) 409
Eco47I GGWCC 3 cut(s) 322, 350, 449
Eco52I CGGCCG 1 cut(s) 286
Eco53kI GAGCTC 1 cut(s) 265
Eco57I CTGAAG 4 cut(s) 935, 1511, 2070, 2933
Eco81I CCTNAGG 1 cut(s) 453
Eco88I CYCGRG 2 cut(s) 244, 2566
Eco91I GGTNACC 1 cut(s) 2273
EcoICRI GAGCTC 1 cut(s) 265
EcoNI CCTNNNNNAGG 1 cut(s) 2788
EcoO65I GGTNACC 1 cut(s) 2273
EcoRII CCWGG 5 cut(s) 499, 744, 1219, 2119, 2706
EcoT14I CCWWGG 5 cut(s) 423, 816, 1346, 1788, 2269
EcoT38I GRGCYC 1 cut(s) 267
ErhI CCWWGG 5 cut(s) 423, 816, 1346, 1788, 2269
FalI AAGNNNNNCTT 6 cut(s) 989, 1021, 1431, 1463, 2072, 2104
FaqI GGGAC 5 cut(s) 56, 727, 1297, 2388, 2980
FauI CCCGC 1 cut(s) 83
FbaI TGATCA 1 cut(s) 1824
FblI GTMKAC 1 cut(s) 1294
Fnu4HI GCNGC 9 cut(s) 121, 160, 207, 210, 286, 1741, 1744, 1976, 2888
FokI GGATG 5 cut(s) 599, 749, 968, 1171, 1286
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 9 cut(s) 121, 160, 207, 210, 286, 1741, 1744, 1976, 2888
FspBI CTAG 5 cut(s) 983, 1310, 1319, 2844, 3089
GlaI GCGC 3 cut(s) 16, 108, 174
GluI GCNGC 9 cut(s) 121, 160, 207, 210, 286, 1741, 1744, 1976, 2888
GsuI CTGGAG 1 cut(s) 2729
HaeIII GGCC 9 cut(s) 150, 288, 428, 1345, 1511, 1653, 1923, 2382, 3014
HgaI GACGC 1 cut(s) 126
HhaI GCGC 3 cut(s) 17, 109, 175
Hin1I GRCGYC 1 cut(s) 333
Hin6I GCGC 3 cut(s) 15, 107, 173
HinP1I GCGC 3 cut(s) 15, 107, 173
HindIII AAGCTT 3 cut(s) 55, 1796, 1993
HphI GGTGA 8 cut(s) 79, 1103, 1373, 1942, 2285, 2819, 2957, 3069
Hpy166II GTNNAC 4 cut(s) 322, 604, 1295, 2580
Hpy8I GTNNAC 4 cut(s) 322, 604, 1295, 2580
Hpy99I CGWCG 8 cut(s) 14, 120, 143, 146, 149, 167, 284, 335
HpyAV CCTTC 9 cut(s) 641, 1333, 1544, 1632, 1646, 2353, 2936, 2974, 3100
HpyCH4IV ACGT 4 cut(s) 45, 333, 1418, 2621
HpyF10VI GCNNNNNNNGC 5 cut(s) 21, 156, 285, 548, 2772
HpySE526I ACGT 4 cut(s) 45, 333, 1418, 2621
Hsp92I GRCGYC 1 cut(s) 333
HspAI GCGC 3 cut(s) 15, 107, 173
KroI GCCGGC 1 cut(s) 2988
KroNI GCCGGC 1 cut(s) 2990
Ksp22I TGATCA 1 cut(s) 1824
KspI CCGCGG 1 cut(s) 160
LguI GCTCTTC 1 cut(s) 375
LmnI GCTCC 5 cut(s) 39, 262, 1502, 1836, 2908
Lsp1109I GCAGC 3 cut(s) 196, 1730, 1962
LweI GCATC 4 cut(s) 901, 967, 1045, 1149
MaeI CTAG 5 cut(s) 983, 1310, 1319, 2844, 3089
MaeII ACGT 4 cut(s) 45, 333, 1418, 2621
MaeIII GTNAC 9 cut(s) 695, 1361, 1414, 1477, 1587, 1694, 1948, 2244, 2273
MbiI CCGCTC 1 cut(s) 348
MfeI CAATTG 4 cut(s) 1059, 1101, 2316, 2550
MflI RGATCY 4 cut(s) 273, 1216, 1351, 2168
MhlI GDGCHC 1 cut(s) 267
MlsI TGGCCA 4 cut(s) 428, 1511, 2382, 3014
MluNI TGGCCA 4 cut(s) 428, 1511, 2382, 3014
MlyI GAGTC 8 cut(s) 138, 187, 194, 236, 1480, 2555, 2981, 3040
MmeI TCCRAC 3 cut(s) 354, 714, 1798
Mox20I TGGCCA 4 cut(s) 428, 1511, 2382, 3014
MroNI GCCGGC 1 cut(s) 2988
MroXI GAANNNNTTC 3 cut(s) 1265, 2043, 2593
MscI TGGCCA 4 cut(s) 428, 1511, 2382, 3014
Msp20I TGGCCA 4 cut(s) 428, 1511, 2382, 3014
MspA1I CMGCKG 1 cut(s) 159
MspCI CTTAAG 1 cut(s) 1805
MspR9I CCNGG 6 cut(s) 501, 746, 1221, 2121, 2708, 2763
MunI CAATTG 4 cut(s) 1059, 1101, 2316, 2550
Mva1269I GAATGC 3 cut(s) 1045, 1230, 2777
MvaI CCWGG 5 cut(s) 501, 746, 1221, 2121, 2708
MvnI CGCG 6 cut(s) 107, 120, 138, 159, 173, 269
MwoI GCNNNNNNNGC 5 cut(s) 21, 156, 285, 548, 2772
NaeI GCCGGC 1 cut(s) 2990
NciI CCSGG 1 cut(s) 2763
NcoI CCATGG 1 cut(s) 423
NgoMIV GCCGGC 1 cut(s) 2988
NlaIV GGNNCC 6 cut(s) 35, 254, 324, 510, 1218, 1344
NmeAIII GCCGAG 3 cut(s) 182, 201, 255
NmuCI GTSAC 5 cut(s) 1361, 1694, 1948, 2244, 2273
NruI TCGCGA 1 cut(s) 269
NspI RCATGY 2 cut(s) 1023, 2873
PaeI GCATGC 1 cut(s) 1023
PaeR7I CTCGAG 1 cut(s) 244
PagI TCATGA 3 cut(s) 376, 1257, 3060
PciI ACATGT 1 cut(s) 2869
PciSI GCTCTTC 1 cut(s) 375
PcsI WCGNNNNNNNCGW 3 cut(s) 124, 1200, 2964
PctI GAATGC 3 cut(s) 1045, 1230, 2777
PdiI GCCGGC 1 cut(s) 2990
PdmI GAANNNNTTC 3 cut(s) 1265, 2043, 2593
Pfl23II CGTACG 2 cut(s) 6, 124
PflFI GACNNNGTC 1 cut(s) 401
PfoI TCCNGGA 1 cut(s) 1219
PinAI ACCGGT 3 cut(s) 1358, 1952, 2094
PkrI GCNGC 9 cut(s) 122, 161, 208, 211, 287, 1742, 1745, 1977, 2889
Ple19I CGATCG 1 cut(s) 195
PleI GAGTC 8 cut(s) 137, 186, 194, 236, 1479, 2554, 2980, 3040
PpsI GAGTC 8 cut(s) 137, 186, 194, 236, 1479, 2554, 2980, 3040
Ppu21I YACGTR 1 cut(s) 1419
PscI ACATGT 1 cut(s) 2869
PshBI ATTAAT 1 cut(s) 1862
PsiI TTATAA 2 cut(s) 2862, 3095
Psp124BI GAGCTC 1 cut(s) 267
Psp6I CCWGG 5 cut(s) 499, 744, 1219, 2119, 2706
PspEI GGTNACC 1 cut(s) 2273
PspGI CCWGG 5 cut(s) 499, 744, 1219, 2119, 2706
PspLI CGTACG 2 cut(s) 6, 124
PspN4I GGNNCC 6 cut(s) 35, 254, 324, 510, 1218, 1344
PspPI GGNCC 4 cut(s) 322, 350, 449, 1343
PspXI VCTCGAGB 1 cut(s) 244
PsrI GAACNNNNNNTAC 2 cut(s) 2125, 2157
PstI CTGCAG 2 cut(s) 1414, 1748
PstNI CAGNNNCTG 1 cut(s) 2248
PsuI RGATCY 4 cut(s) 273, 1216, 1351, 2168
PsyI GACNNNGTC 1 cut(s) 401
PvuI CGATCG 1 cut(s) 195
RruI TCGCGA 1 cut(s) 269
RsaI GTAC 5 cut(s) 8, 126, 1421, 1888, 2311
RsaNI GTAC 5 cut(s) 7, 125, 1420, 1887, 2310
Rsr2I CGGWCCG 1 cut(s) 350
RsrII CGGWCCG 1 cut(s) 350
SacI GAGCTC 1 cut(s) 267
SacII CCGCGG 1 cut(s) 160
SapI GCTCTTC 1 cut(s) 375
SatI GCNGC 9 cut(s) 121, 160, 207, 210, 286, 1741, 1744, 1976, 2888
Sau96I GGNCC 4 cut(s) 322, 350, 449, 1343
SchI GAGTC 8 cut(s) 138, 187, 194, 236, 1480, 2555, 2981, 3040
ScrFI CCNGG 6 cut(s) 501, 746, 1221, 2121, 2708, 2763
SduI GDGCHC 1 cut(s) 267
SfaNI GCATC 4 cut(s) 901, 967, 1045, 1149
SfcI CTRYAG 3 cut(s) 1410, 1744, 2225
Sfr274I CTCGAG 1 cut(s) 244
Sfr303I CCGCGG 1 cut(s) 160
SgrBI CCGCGG 1 cut(s) 160
SinI GGWCC 3 cut(s) 322, 350, 449
SlaI CTCGAG 1 cut(s) 244
SmlI CTYRAG 9 cut(s) 244, 257, 524, 1805, 1934, 2216, 2498, 2507, 2641
SmoI CTYRAG 9 cut(s) 244, 257, 524, 1805, 1934, 2216, 2498, 2507, 2641
SnaBI TACGTA 1 cut(s) 1419
SphI GCATGC 1 cut(s) 1023
SspMI CTAG 5 cut(s) 983, 1310, 1319, 2844, 3089
SstI GAGCTC 1 cut(s) 267
StyD4I CCNGG 6 cut(s) 499, 744, 1219, 2119, 2706, 2761
StyI CCWWGG 5 cut(s) 423, 816, 1346, 1788, 2269
TaiI ACGT 4 cut(s) 48, 336, 1421, 2624
TaqI TCGA 5 cut(s) 111, 195, 245, 937, 1841
TatI WGTACW 1 cut(s) 1886
TauI GCSGC 6 cut(s) 123, 162, 209, 288, 1743, 2890
TscAI CASTG 5 cut(s) 24, 780, 1330, 1762, 2253
TseFI GTSAC 5 cut(s) 1361, 1694, 1948, 2244, 2273
TseI GCWGC 3 cut(s) 209, 1743, 1975
Tsp45I GTSAC 5 cut(s) 1361, 1694, 1948, 2244, 2273
TspRI CASTG 5 cut(s) 24, 780, 1330, 1762, 2253
Tth111I GACNNNGTC 1 cut(s) 401
Vha464I CTTAAG 1 cut(s) 1805
VpaK11BI GGWCC 3 cut(s) 322, 350, 449
VspI ATTAAT 1 cut(s) 1862
XagI CCTNNNNNAGG 1 cut(s) 2788
XapI RAATTY 6 cut(s) 706, 1368, 1543, 1562, 1849, 2294
XceI RCATGY 2 cut(s) 1023, 2873
XcmI CCANNNNNNNNNTGG 2 cut(s) 1841, 2203
XhoI CTCGAG 1 cut(s) 244
XmiI GTMKAC 1 cut(s) 1294
XmnI GAANNNNTTC 3 cut(s) 1265, 2043, 2593
XspI CTAG 5 cut(s) 983, 1310, 1319, 2844, 3089
ZraI GACGTC 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.