AT1G27170

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001217
Physical Location & Seq
Forward (+)
9433393 .. 9439417
6025 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G27170.2

Sequence Viewer

Length: 4155 bp
ATGGAATCTGGCGTTGTTTCGAAGCCGCATAGGCTCAAGTATGACGTATTCCTCAGTTTCCGTGGCGCAGACACGCGCGATAACTTCGGAGACCATCTCTATAAAGCGCTCAAGGATAAAGTTCGGGTCTTCCGCGACAACGAAGGTATGGAACGCGGAGATGAGATCAGTTCAAGTCTTAAGGCAGGCATGGAGGACTCTGCGGCCTCTGTCATCGTCATATCGCGTAACTACTCTGGCTCTCGCTGGTGCCTTGATGAATTAGCTATGCTCTGCAAGATGAAATCATCTCTAGATCGTCGAATCTTACCCATTTTTTATCATGTCGATCCCTCGCATGTTCGCAAACAGAGCGATCATATCAAGAAGGATTTTGAAGAACACCAAGTAAGATTTAGCGAGGAGAAAGAGAAGGTACAAGAATGGAGAGAAGCTTTGACATTAGTTGGGAATCTTGCTGGATATGTTTGCGACAAGGACTCGAAGGATGATGATATGATAGAGCTTGTGGTGAAAAGGGTTTTAGCTGAATTGAGCAATACACCAGAGAAAGTGGGAGAATTCATTGTTGGTCTGGAATCACCTTTAAAGGATTTGACGGGCCTGATCGACACTGAATCCAGTTCTGGCGTCCAAGTCTTGGGACTTTATGGTATGGGTGGTATTGGGAAGACTACCCTTGCAAAAGCCTTTTATAACAAGATTGTCGGAAACTTCGAGCAACGAGCTTTCATCTCAGACATTAGAGAAAGATCATCAGCCGAAAATGGGTTAGTCACTCTTCAAAAAACTCTCATCAAGGAACTTTTCCGTTTGGTACCTGAAATAGAGGATGTTAGCATAGGCCTGGAAAAGATAAAAGCAAATGTTCACGAGAAGAAGATTATTGTGGTTTTAGATGATGTTGATCACATAGACCAGGTTCATGCCCTGGTTGGTGAAACAAGATGGTATGGTCAAGGAACACTTATAGTAATCACTACTAGAGATTCTGAGATTCTGAGTAAGCTCTCAGTGAACCAACAGTATGAGGTCAAGTGCTTGACTGAGCCGCAGGCATTGAAGCTCTTTAGTTATCATTCACTACGAAAAGAAGAACCAACAAAGAATCTGTTGGCGTTATCCAAGAAGATTGTCCAGATATCAGGACTATTGCCGCTTGCAGTTGAAGTTTTTGGATCTCTTTTGTATGACAAGAAGGAGGAAAAGGATTGGCAAACTCAACTGGATAAGCTGAAAAAGACTCAACCAGGCAATCTTCAGGATGTTCTGGAACTGAGTTTCAAATCTTTAGACGATGAAGAAAAGAAAGTATTCCTCGACATTGCATGTCTCTTTCTTAAAATGGAGATAAAAAAAGACGAAGTTGTCATTGTACTGAAAGGATGTGGGTTAAACGCTGAGGCTGCTCTCAGTGTCCTTAGACAGAAATCTCTTGTTAAGATCTTGGCAAACGATACTCTTTGGATGCATGATCAGATTAGAGACATGGGTAGGCAGATGGTCCTTAAAGAAAGCCGTGAGGATCCGGGATTGCGAAGTAGACTCTGGGATCGTGGTGAAATTATGACCGTACTGAACAATATGAAGGGAACATCATCCATTCGAGGAATCGTACTTGACTTTAAAAAAAAGTTTGCTAGGGACCCTACTGCAGATGAAATTGTCTCGAGGAATCTACGCAACAATCCGGGTATCTACTCTGTATTCAATTACCTGAAGAATAAGTTAGTAAGATTTCCAGCAGAGGAAAAGCCAAAAAGTTCTGAAATCACCATTCCCGTAGAATCTTTTGCACCAATGACGAAGTTGAGACTTCTTCAGATTAATAACGTGGAACTGGAAGGAAATCTTAAACTTCTCCCATCTGAACTCAAGTGGATACAGTGGAAAGGTTGCCCCTTAGAAAATCTCCCTCCGGATTTTCTTGCTAGGCAACTTTCTGTTCTTGATCTTTCAGAGAGTGGAATAAGACAAGTCCAGACTTTGCGCAACAAAATGGTGGATGAGAATTTGAAGGTTGTAATTTTGCGTGGTTGCCACAGCTTAGAAGCCATTCCTGATTTATCAAACCATGAAGCCCTAGAGAAGCTTGTTTTCGAGCAATGCACCCTTCTGGTGAAGGTTCCTAAATCAGTTGGTAATCTGAGAAAATTGATTCACCTGGACTTCAGAAGGTGTTCAAAGCTTTCTGAATTTCTTGTGGATGTTTCTGGACTTAAGCTTCTTGAAAAACTATTCCTCTCTGGCTGTTCAGATCTGAGTGTGTTACCAGAAAACATTGGTGCCATGACAAGCTTGAAAGAGCTTCTTCTTGATGGAACTGCGATAAAGAACTTACCAGAATCTATAAATCGCCTCCAAAATCTTGAAATCCTTAGTCTAAGAGGTTGCAAAATTCAAGAGCTACCTTTGTGCATAGGAACATTGAAATCGCTGGAGAAGCTATATCTTGATGATACTGCGTTGAAAAATCTTCCGAGTTCTATTGGAGATCTAAAAAATCTCCAGGACCTCCATTTGGTACGCTGCACATCCCTTTCTAAGATTCCTGACAGTATCAATGAGCTCAAATCTTTGAAGAAACTATTCATCAACGGAAGCGCGGTGGAGGAATTACCTCTAAAACCGAGTTCACTGCCAAGCTTGTATGACTTCTCAGCAGGAGATTGCAAATTTCTGAAACAGGTTCCGAGTTCAATTGGTAGATTAAATTCTCTTCTCCAACTTCAGTTGAGTAGCACCCCAATTGAAGCTCTACCAGAAGAGATTGGTGCCTTGCATTTTATACGCGAACTTGAGTTGAGAAACTGCAAATTTCTGAAGTTTCTGCCTAAGTCAATTGGAGATATGGACACACTCTATAGCTTAAACCTTGAAGGCTCTAACATTGAGGAACTGCCGGAAGAATTTGGCAAGCTGGAAAAGCTAGTTGAACTGCGAATGAGCAACTGTAAAATGCTCAAGAGGCTTCCTGAATCGTTTGGAGACTTGAAATCTCTTCACCGTCTGTACATGAAGGAAACTTTGGTCTCAGAGTTGCCAGAAAGTTTCGGAAACCTCTCAAATTTAATGGTACTGGAAATGCTGAAGAAGCCTCTTTTTAGAATTTCTGAGAGTAATGTTCCAGGTACAAGCGAAGAACCTCGTTTTGTAGAAGTACCAAACTCTTTCTCAAAGCTCTTGAAGCTTGAGGAACTAGATGCTTGCAGCTGGAGAATATCGGGTAAAATCCCTGATGATCTTGAGAAGCTTTCGTGTTTGATGAAACTGAATCTGGGGAATAATTATTTTCATAGTCTTCCGTCTAGCCTCGTAAAGTTATCAAATCTCCAAGAACTTTCATTACGTGACTGCCGAGAGCTCAAGCGTCTTCCCCCTCTTCCATGTAAACTGGAGCAGCTAAACCTAGCAAACTGTTTCTCATTGGAGAGTGTATCCGACCTTTCAGAGTTAACGATCTTGACGGATCTCAATCTCACAAACTGTGCGAAAGTGGTTGATATTCCAGGCCTAGAGCACTTGACGGCTCTGAAAAGATTATACATGACCGGATGTAACTCCAACTACTCCCTTGCAGTAAAGAAAAGACTTTCCAAGGCTTCTTTGAAGATGATGCGGAATCTGAGCTTGCCTGGAAACAGGGTCCCGGACTGGTTCTCGCAAGGCCCTGTCACATTCTCAGCTCAACCGAACAGAGAGCTCAGAGGTGTAATCATTGCTGTTGTTGTGGCTCTTAATGATGAAACCGAAGATGACGACTATCAGCTGCCTGATGTGATGGAGGTTCAAGCCCAAATTCATAAACTTGATCATCACAAATGCACCAACACATTGCATCTCTCCGGAGTGCCTAGAACAAATAACGATCAGCTTCACATCTGCCGATACTCAGCTTTTCACCCACTGGTTACGATGTTGAAAGACGGGTACACCATACAGGTGATCAAACGAAACCCACCAATCAAACAAGGCGTTGAGCTAAAGATGCATGGCATTCATCTCGTTTACGAGGGGGATGATGATTTGGAAGGCAGAGAAAATACTTTACCCGAGACTCAACAAACCGTTTCTCAGAAACTTGCCAATTTTTTCAGCTCTTTCGAAGAAGAAGAAGGTGAAACCATCTCAGAAAGTGAATCTACAGTTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1384

Amino Acids

156.37

Weight (kDa)

6.13

Isoelectric Point (pI)

40.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 16 - 184 3.2e-34 TIR domain
TIR_2 PF13676 18 - 128 8.2e-13 TIR domain
ATPase_2 PF01637 198 - 306 6.2e-06 ATPase domain predominantly from Archaea
NB-ARC PF00931 208 - 364 1.5e-18 NB-ARC domain
WHD_ROQ1 PF23282 433 - 505 8.5e-16 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 736 - 866 7.2e-19 Leucine-rich repeat region
LRR_14 PF23598 899 - 958 1.6e-07 Leucine-rich repeat region
LRR_14 PF23598 957 - 1038 5.4e-10 Leucine-rich repeat region
LRR_14 PF23598 1076 - 1210 9.3e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 696
AasI GACNNNNNNGTC 1 cut(s) 1367
Acc16I TGCGCA 1 cut(s) 1991
Acc65I GGTACC 1 cut(s) 817
AccB1I GGYRCC 4 cut(s) 249, 817, 2285, 2774
AccB7I CCANNNNNTGG 1 cut(s) 640
AccI GTMKAC 1 cut(s) 1543
AccII CGCG 7 cut(s) 76, 78, 135, 156, 226, 2606, 2793
AccIII TCCGGA 2 cut(s) 1918, 3845
AciI CCGC 8 cut(s) 26, 133, 156, 203, 1052, 1157, 2606, 3619
AclWI GGATC 6 cut(s) 323, 1186, 1520, 1533, 1560, 3477
AcuI CTGAAG 7 cut(s) 1244, 1739, 1805, 2155, 2714, 2843, 3110
AcyI GRCGYC 1 cut(s) 630
AfeI AGCGCT 1 cut(s) 108
AfiI CCNNNNNNNGG 3 cut(s) 640, 768, 2521
AflII CTTAAG 2 cut(s) 179, 2219
AjnI CCWGG 9 cut(s) 846, 918, 930, 1249, 2163, 2508, 3127, 3508, 3634
AjuI GAANNNNNNNTTGG 4 cut(s) 552, 584, 3011, 3043
Alw21I GWGCWC 4 cut(s) 2571, 3366, 3522, 3705
Alw26I GTCTC 8 cut(s) 84, 1337, 1479, 1672, 1807, 2982, 3037, 4049
AlwI GGATC 6 cut(s) 323, 1186, 1520, 1533, 1560, 3477
Ama87I CYCGRG 2 cut(s) 1669, 4052
Aor13HI TCCGGA 2 cut(s) 1918, 3845
Aor51HI AGCGCT 1 cut(s) 108
AoxI GGCC 5 cut(s) 204, 601, 844, 3511, 3667
ApeKI GCWGC 5 cut(s) 1406, 2529, 3210, 3400, 3769
AseI ATTAAT 1 cut(s) 1827
Asp700I GAANNNNTTC 4 cut(s) 1313, 2055, 2179, 2588
Asp718I GGTACC 1 cut(s) 817
AspLEI GCGC 5 cut(s) 68, 78, 109, 1992, 2606
AspS9I GGNCC 6 cut(s) 601, 1504, 1645, 2512, 3646, 3668
AsuC2I CCSGG 3 cut(s) 1530, 1692, 3650
AsuII TTCGAA 2 cut(s) 20, 4104
AvaI CYCGRG 2 cut(s) 1669, 4052
AvaII GGWCC 4 cut(s) 1504, 1645, 2512, 3646
BamHI GGATCC 1 cut(s) 1525
BanI GGYRCC 4 cut(s) 249, 817, 2285, 2774
BanII GRGCYC 3 cut(s) 2571, 3366, 3705
BauI CACGAG 1 cut(s) 872
BbsI GAAGAC 4 cut(s) 121, 677, 3293, 3365
Bbv12I GWGCWC 4 cut(s) 2571, 3366, 3522, 3705
BbvCI CCTCAGC 1 cut(s) 1401
BbvI GCAGC 5 cut(s) 1393, 2516, 3222, 3412, 3756
BccI CCATC 7 cut(s) 102, 942, 1495, 1873, 2312, 3775, 4133
BceAI ACGGC 2 cut(s) 1503, 3543
BcgI CGANNNNNNTGC 4 cut(s) 2620, 2654, 3985, 4019
BciT130I CCWGG 9 cut(s) 848, 920, 932, 1251, 2165, 2510, 3129, 3510, 3636
BciVI GTATCC 2 cut(s) 1875, 3448
BclI TGATCA 4 cut(s) 907, 1474, 3811, 3945
BcnI CCSGG 3 cut(s) 1530, 1692, 3650
BcoDI GTCTC 8 cut(s) 84, 1337, 1479, 1672, 1807, 2982, 3037, 4049
BfmI CTRYAG 3 cut(s) 1653, 2863, 4143
BfoI RGCGCY 1 cut(s) 110
BfrI CTTAAG 2 cut(s) 179, 2219
BfuI GTATCC 2 cut(s) 1875, 3448
BglI GCCNNNNNGGC 1 cut(s) 31
BglII AGATCT 3 cut(s) 1443, 2257, 2494
BisI GCNGC 9 cut(s) 26, 204, 1052, 1157, 1407, 2530, 3211, 3401, 3770
BlsI GCNGC 9 cut(s) 27, 205, 1053, 1158, 1408, 2531, 3212, 3402, 3771
Bme18I GGWCC 4 cut(s) 1504, 1645, 2512, 3646
BmeT110I CYCGRG 2 cut(s) 1669, 4052
BmgT120I GGNCC 6 cut(s) 601, 1504, 1645, 2512, 3646, 3668
BmsI GCATC 5 cut(s) 1458, 3193, 3606, 3847, 3978
BpiI GAAGAC 4 cut(s) 121, 677, 3293, 3365
BplI GAGNNNNNCTC 2 cut(s) 81, 113
BpmI CTGGAG 4 cut(s) 2459, 2492, 3235, 3416
Bpu10I CCTNAGC 1 cut(s) 1401
Bpu14I TTCGAA 2 cut(s) 20, 4104
BpuEI CTTGAG 8 cut(s) 20, 95, 1859, 2819, 2948, 3212, 3266, 3350
BpuMI CCSGG 3 cut(s) 1530, 1692, 3650
BsaAI YACGTR 1 cut(s) 3350
BsaBI GATNNNNATC 2 cut(s) 906, 3474
BsaHI GRCGYC 1 cut(s) 630
BsaI GGTCTC 2 cut(s) 84, 3037
BsaJI CCNNGG 3 cut(s) 61, 930, 3597
BsaWI WCCGGW 3 cut(s) 1918, 3551, 3845
Bsc4I CCNNNNNNNGG 3 cut(s) 640, 768, 2521
Bse1I ACTGG 7 cut(s) 621, 1230, 1845, 3084, 3399, 3659, 3912
Bse3DI GCAATG 4 cut(s) 1323, 2111, 3717, 3833
Bse8I GATNNNNATC 2 cut(s) 906, 3474
BseAI TCCGGA 2 cut(s) 1918, 3845
BseBI CCWGG 9 cut(s) 848, 920, 932, 1251, 2165, 2510, 3129, 3510, 3636
BseDI CCNNGG 3 cut(s) 61, 930, 3597
BseJI GATNNNNATC 2 cut(s) 906, 3474
BseLI CCNNNNNNNGG 3 cut(s) 640, 768, 2521
BseMI GCAATG 4 cut(s) 1323, 2111, 3717, 3833
BseNI ACTGG 7 cut(s) 621, 1230, 1845, 3084, 3399, 3659, 3912
BseRI GAGGAG 1 cut(s) 416
BseXI GCAGC 5 cut(s) 1393, 2516, 3222, 3412, 3756
BsgI GTGCAG 1 cut(s) 2515
Bsh1236I CGCG 7 cut(s) 76, 78, 135, 156, 226, 2606, 2793
BshFI GGCC 5 cut(s) 206, 603, 846, 3513, 3669
BshNI GGYRCC 4 cut(s) 249, 817, 2285, 2774
BsiHKAI GWGCWC 4 cut(s) 2571, 3366, 3522, 3705
BsiHKCI CYCGRG 2 cut(s) 1669, 4052
BsiSI CCGG 7 cut(s) 1529, 1691, 1919, 2903, 3552, 3650, 3846
BslFI GGGAC 3 cut(s) 657, 1658, 3632
BslI CCNNNNNNNGG 3 cut(s) 640, 768, 2521
BsmAI GTCTC 8 cut(s) 84, 1337, 1479, 1672, 1807, 2982, 3037, 4049
BsmFI GGGAC 3 cut(s) 657, 1658, 3632
BsmI GAATGC 1 cut(s) 3996
BsnI GGCC 5 cut(s) 206, 603, 846, 3513, 3669
Bso31I GGTCTC 2 cut(s) 84, 3037
BsoBI CYCGRG 2 cut(s) 1669, 4052
Bsp119I TTCGAA 2 cut(s) 20, 4104
Bsp1286I GDGCHC 4 cut(s) 2571, 3366, 3522, 3705
Bsp13I TCCGGA 2 cut(s) 1918, 3845
Bsp1407I TGTACA 1 cut(s) 3012
BspACI CCGC 8 cut(s) 26, 133, 156, 203, 1052, 1157, 2606, 3619
BspANI GGCC 5 cut(s) 206, 603, 846, 3513, 3669
BspEI TCCGGA 2 cut(s) 1918, 3845
BspFNI CGCG 7 cut(s) 76, 78, 135, 156, 226, 2606, 2793
BspMAI CTGCAG 1 cut(s) 1657
BspPI GGATC 6 cut(s) 323, 1186, 1520, 1533, 1560, 3477
BspT104I TTCGAA 2 cut(s) 20, 4104
BspT107I GGYRCC 4 cut(s) 249, 817, 2285, 2774
BspTI CTTAAG 2 cut(s) 179, 2219
BspTNI GGTCTC 2 cut(s) 84, 3037
BsrDI GCAATG 4 cut(s) 1323, 2111, 3717, 3833
BsrGI TGTACA 1 cut(s) 3012
BsrI ACTGG 7 cut(s) 621, 1230, 1845, 3084, 3399, 3659, 3912
BssECI CCNNGG 3 cut(s) 61, 930, 3597
BssNI GRCGYC 1 cut(s) 630
BssSI CACGAG 1 cut(s) 872
BssT1I CCWWGG 1 cut(s) 3597
Bst2BI CACGAG 1 cut(s) 872
Bst2UI CCWGG 9 cut(s) 848, 920, 932, 1251, 2165, 2510, 3129, 3510, 3636
Bst6I CTCTTC 5 cut(s) 786, 2724, 2760, 3006, 3387
BstACI GRCGYC 1 cut(s) 630
BstAFI CTTAAG 2 cut(s) 179, 2219
BstAUI TGTACA 1 cut(s) 3012
BstBAI YACGTR 1 cut(s) 3350
BstBI TTCGAA 2 cut(s) 20, 4104
BstC8I GCNNGC 6 cut(s) 187, 1056, 1161, 2918, 3208, 3632
BstDSI CCRYGG 1 cut(s) 61
BstFNI CGCG 7 cut(s) 76, 78, 135, 156, 226, 2606, 2793
BstH2I RGCGCY 1 cut(s) 110
BstHHI GCGC 5 cut(s) 68, 78, 109, 1992, 2606
BstMAI GTCTC 8 cut(s) 84, 1337, 1479, 1672, 1807, 2982, 3037, 4049
BstMWI GCNNNNNNNGC 9 cut(s) 31, 351, 1406, 2443, 2926, 2968, 3094, 3187, 3988
BstNI CCWGG 9 cut(s) 848, 920, 932, 1251, 2165, 2510, 3129, 3510, 3636
BstNSI RCATGY 2 cut(s) 341, 1332
BstSFI CTRYAG 3 cut(s) 1653, 2863, 4143
BstUI CGCG 7 cut(s) 76, 78, 135, 156, 226, 2606, 2793
BstV1I GCAGC 5 cut(s) 1393, 2516, 3222, 3412, 3756
BstV2I GAAGAC 4 cut(s) 121, 677, 3293, 3365
BstX2I RGATCY 6 cut(s) 1178, 1443, 1525, 2257, 2494, 3469
BstYI RGATCY 6 cut(s) 1178, 1443, 1525, 2257, 2494, 3469
BsuI GTATCC 2 cut(s) 1875, 3448
BsuRI GGCC 5 cut(s) 206, 603, 846, 3513, 3669
BtgI CCRYGG 1 cut(s) 61
BtsI GCAGTG 1 cut(s) 2636
BtsIMutI CAGTG 6 cut(s) 612, 1020, 1420, 1892, 2636, 3905
Cac8I GCNNGC 6 cut(s) 187, 1056, 1161, 2918, 3208, 3632
CfoI GCGC 5 cut(s) 68, 78, 109, 1992, 2606
Cfr13I GGNCC 6 cut(s) 601, 1504, 1645, 2512, 3646, 3668
CseI GACGC 2 cut(s) 619, 3359
CsiI ACCWGGT 1 cut(s) 918
DraI TTTAAA 2 cut(s) 588, 1627
DrdI GACNNNNNNGTC 1 cut(s) 1367
DseDI GACNNNNNNGTC 1 cut(s) 1367
Eam1104I CTCTTC 5 cut(s) 786, 2724, 2760, 3006, 3387
EarI CTCTTC 5 cut(s) 786, 2724, 2760, 3006, 3387
Ecl136II GAGCTC 3 cut(s) 2569, 3364, 3703
Eco130I CCWWGG 1 cut(s) 3597
Eco147I AGGCCT 2 cut(s) 846, 3513
Eco24I GRGCYC 3 cut(s) 2571, 3366, 3705
Eco31I GGTCTC 2 cut(s) 84, 3037
Eco32I GATATC 1 cut(s) 1143
Eco47I GGWCC 4 cut(s) 1504, 1645, 2512, 3646
Eco47III AGCGCT 1 cut(s) 108
Eco53kI GAGCTC 3 cut(s) 2569, 3364, 3703
Eco57I CTGAAG 7 cut(s) 1244, 1739, 1805, 2155, 2714, 2843, 3110
Eco88I CYCGRG 2 cut(s) 1669, 4052
EcoICRI GAGCTC 3 cut(s) 2569, 3364, 3703
EcoO109I RGGNCCY 4 cut(s) 1645, 2512, 3646, 3668
EcoRI GAATTC 1 cut(s) 560
EcoRII CCWGG 9 cut(s) 846, 918, 930, 1249, 2163, 2508, 3127, 3508, 3634
EcoRV GATATC 1 cut(s) 1143
EcoT14I CCWWGG 1 cut(s) 3597
EcoT22I ATGCAT 2 cut(s) 1473, 3993
EcoT38I GRGCYC 3 cut(s) 2571, 3366, 3705
ErhI CCWWGG 1 cut(s) 3597
FalI AAGNNNNNCTT 6 cut(s) 951, 983, 1836, 1868, 2295, 2327
FaqI GGGAC 3 cut(s) 657, 1658, 3632
FbaI TGATCA 4 cut(s) 907, 1474, 3811, 3945
FblI GTMKAC 1 cut(s) 1543
Fnu4HI GCNGC 9 cut(s) 26, 204, 1052, 1157, 1407, 2530, 3211, 3401, 3770
FriOI GRGCYC 3 cut(s) 2571, 3366, 3705
Fsp4HI GCNGC 9 cut(s) 26, 204, 1052, 1157, 1407, 2530, 3211, 3401, 3770
FspI TGCGCA 1 cut(s) 1991
GlaI GCGC 5 cut(s) 67, 77, 108, 1991, 2605
GluI GCNGC 9 cut(s) 26, 204, 1052, 1157, 1407, 2530, 3211, 3401, 3770
GsuI CTGGAG 4 cut(s) 2459, 2492, 3235, 3416
HaeII RGCGCY 1 cut(s) 110
HaeIII GGCC 5 cut(s) 206, 603, 846, 3513, 3669
HapII CCGG 7 cut(s) 1529, 1691, 1919, 2903, 3552, 3650, 3846
HgaI GACGC 2 cut(s) 619, 3359
HhaI GCGC 5 cut(s) 68, 78, 109, 1992, 2606
Hin1I GRCGYC 1 cut(s) 630
Hin6I GCGC 5 cut(s) 66, 76, 107, 1990, 2604
HinP1I GCGC 5 cut(s) 66, 76, 107, 1990, 2604
HincII GTYRAC 1 cut(s) 3456
HindII GTYRAC 1 cut(s) 3456
HindIII AAGCTT 8 cut(s) 432, 2090, 2186, 2222, 2296, 2644, 3188, 3251
HpaI GTTAAC 1 cut(s) 3456
HpaII CCGG 7 cut(s) 1529, 1691, 1919, 2903, 3552, 3650, 3846
Hpy166II GTNNAC 8 cut(s) 871, 1018, 1544, 2636, 3392, 3456, 3933, 4009
Hpy8I GTNNAC 8 cut(s) 871, 1018, 1544, 2636, 3392, 3456, 3933, 4009
Hpy99I CGWCG 1 cut(s) 303
HpyCH4IV ACGT 3 cut(s) 45, 1833, 3349
HpyF10VI GCNNNNNNNGC 9 cut(s) 31, 351, 1406, 2443, 2926, 2968, 3094, 3187, 3988
HpySE526I ACGT 3 cut(s) 45, 1833, 3349
Hsp92I GRCGYC 1 cut(s) 630
HspAI GCGC 5 cut(s) 66, 76, 107, 1990, 2604
KflI GGGWCCC 2 cut(s) 1645, 3646
Kpn2I TCCGGA 2 cut(s) 1918, 3845
KpnI GGTACC 1 cut(s) 821
Ksp22I TGATCA 4 cut(s) 907, 1474, 3811, 3945
KspAI GTTAAC 1 cut(s) 3456
LmnI GCTCC 1 cut(s) 3397
Lsp1109I GCAGC 5 cut(s) 1393, 2516, 3222, 3412, 3756
LweI GCATC 5 cut(s) 1458, 3193, 3606, 3847, 3978
MabI ACCWGGT 1 cut(s) 918
MaeII ACGT 3 cut(s) 45, 1833, 3349
MaeIII GTNAC 7 cut(s) 227, 775, 2268, 3350, 3557, 3673, 3910
MfeI CAATTG 3 cut(s) 2700, 2748, 2841
MflI RGATCY 6 cut(s) 1178, 1443, 1525, 2257, 2494, 3469
MhlI GDGCHC 4 cut(s) 2571, 3366, 3522, 3705
MlyI GAGTC 5 cut(s) 191, 473, 1237, 1539, 4051
MmeI TCCRAC 4 cut(s) 688, 2749, 3465, 3588
Mph1103I ATGCAT 2 cut(s) 1473, 3993
MroI TCCGGA 2 cut(s) 1918, 3845
MroXI GAANNNNTTC 4 cut(s) 1313, 2055, 2179, 2588
MslI CAYNNNNRTG 1 cut(s) 3941
MspA1I CMGCKG 2 cut(s) 3213, 3769
MspCI CTTAAG 2 cut(s) 179, 2219
MspI CCGG 7 cut(s) 1529, 1691, 1919, 2903, 3552, 3650, 3846
MunI CAATTG 3 cut(s) 2700, 2748, 2841
Mva1269I GAATGC 1 cut(s) 3996
MvaI CCWGG 9 cut(s) 848, 920, 932, 1251, 2165, 2510, 3129, 3510, 3636
MvnI CGCG 7 cut(s) 76, 78, 135, 156, 226, 2606, 2793
MwoI GCNNNNNNNGC 9 cut(s) 31, 351, 1406, 2443, 2926, 2968, 3094, 3187, 3988
NciI CCSGG 3 cut(s) 1530, 1692, 3650
NmeAIII GCCGAG 1 cut(s) 3383
NmuCI GTSAC 3 cut(s) 775, 3350, 3673
NsbI TGCGCA 1 cut(s) 1991
NsiI ATGCAT 2 cut(s) 1473, 3993
NspI RCATGY 2 cut(s) 341, 1332
NspV TTCGAA 2 cut(s) 20, 4104
PaeR7I CTCGAG 1 cut(s) 1669
PceI AGGCCT 2 cut(s) 846, 3513
PcsI WCGNNNNNNNCGW 2 cut(s) 714, 3355
PctI GAATGC 1 cut(s) 3996
PdmI GAANNNNTTC 4 cut(s) 1313, 2055, 2179, 2588
PflMI CCANNNNNTGG 1 cut(s) 640
PfoI TCCNGGA 3 cut(s) 1528, 2508, 3648
PkrI GCNGC 9 cut(s) 27, 205, 1053, 1158, 1408, 2531, 3212, 3402, 3771
PleI GAGTC 5 cut(s) 191, 473, 1237, 1539, 4051
PpsI GAGTC 5 cut(s) 191, 473, 1237, 1539, 4051
Ppu21I YACGTR 1 cut(s) 3350
PpuMI RGGWCCY 3 cut(s) 1645, 2512, 3646
PshBI ATTAAT 1 cut(s) 1827
PsiI TTATAA 1 cut(s) 696
Psp124BI GAGCTC 3 cut(s) 2571, 3366, 3705
Psp5II RGGWCCY 3 cut(s) 1645, 2512, 3646
Psp6I CCWGG 9 cut(s) 846, 918, 930, 1249, 2163, 2508, 3127, 3508, 3634
PspGI CCWGG 9 cut(s) 846, 918, 930, 1249, 2163, 2508, 3127, 3508, 3634
PspPI GGNCC 6 cut(s) 601, 1504, 1645, 2512, 3646, 3668
PspPPI RGGWCCY 3 cut(s) 1645, 2512, 3646
PsrI GAACNNNNNNTAC 2 cut(s) 3330, 3362
PstI CTGCAG 1 cut(s) 1657
PsuI RGATCY 6 cut(s) 1178, 1443, 1525, 2257, 2494, 3469
PvuII CAGCTG 2 cut(s) 3213, 3769
RseI CAYNNNNRTG 1 cut(s) 3941
SacI GAGCTC 3 cut(s) 2571, 3366, 3705
SatI GCNGC 9 cut(s) 26, 204, 1052, 1157, 1407, 2530, 3211, 3401, 3770
Sau96I GGNCC 6 cut(s) 601, 1504, 1645, 2512, 3646, 3668
SchI GAGTC 5 cut(s) 191, 473, 1237, 1539, 4051
SduI GDGCHC 4 cut(s) 2571, 3366, 3522, 3705
SexAI ACCWGGT 1 cut(s) 918
SfaNI GCATC 5 cut(s) 1458, 3193, 3606, 3847, 3978
SfcI CTRYAG 3 cut(s) 1653, 2863, 4143
Sfr274I CTCGAG 1 cut(s) 1669
SfuI TTCGAA 2 cut(s) 20, 4104
SinI GGWCC 4 cut(s) 1504, 1645, 2512, 3646
SlaI CTCGAG 1 cut(s) 1669
SmiMI CAYNNNNRTG 1 cut(s) 3941
SseBI AGGCCT 2 cut(s) 846, 3513
SsiI CCGC 8 cut(s) 26, 133, 156, 203, 1052, 1157, 2606, 3619
SstI GAGCTC 3 cut(s) 2571, 3366, 3705
StuI AGGCCT 2 cut(s) 846, 3513
StyI CCWWGG 1 cut(s) 3597
TaiI ACGT 3 cut(s) 48, 1836, 3352
TatI WGTACW 2 cut(s) 1375, 3012
TauI GCSGC 4 cut(s) 28, 206, 1054, 1159
TscAI CASTG 6 cut(s) 619, 1020, 1420, 1892, 2643, 3912
TseFI GTSAC 3 cut(s) 775, 3350, 3673
TseI GCWGC 5 cut(s) 1406, 2529, 3210, 3400, 3769
Tsp45I GTSAC 3 cut(s) 775, 3350, 3673
TspGWI ACGGA 5 cut(s) 50, 800, 2613, 3294, 3482
TspRI CASTG 6 cut(s) 619, 1020, 1420, 1892, 2643, 3912
Van91I CCANNNNNTGG 1 cut(s) 640
Vha464I CTTAAG 2 cut(s) 179, 2219
VpaK11BI GGWCC 4 cut(s) 1504, 1645, 2512, 3646
VspI ATTAAT 1 cut(s) 1827
XbaI TCTAGA 1 cut(s) 292
XceI RCATGY 2 cut(s) 341, 1332
XhoI CTCGAG 1 cut(s) 1669
XmiI GTMKAC 1 cut(s) 1543
XmnI GAANNNNTTC 4 cut(s) 1313, 2055, 2179, 2588
Zsp2I ATGCAT 2 cut(s) 1473, 3993
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.