MD16G1082300.v1.1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001217
Physical Location & Seq
Reverse (-)
5774698 .. 5776714
2017 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1082300.v1.1.491

Sequence Viewer

Length: 1761 bp
ATGAGGTTGTTAGATGTTCGATCCAATGGCGTTCGAGTGCTCGGAATTCATGGCATGGGTGGTGTTGGCAAGACGACTCTTGCCAAGGCCCTTTTTAACAGACTTGTTGCTTACTTCGAGCATCGCGGCTTCATCTCAAATGTTAGAGAAACTTCTGCAGGACACGAGGGGTTAGTATCACTTCAAAACAGACTTATCGGTAGTCTTTCTACTGTGAAGCGAATTCTTATTGTTTTGGATGATGTTGATAATGTAGAGCAACTAAGTGCTTTAGTAGGCAACAGAGACTGGTTTTATGAAGGAAGCAGAATCATTGTTACCACAAGAGATAGAAGTGTCTTGCCCGATCATCTTGTGAACAAGTTGTATGAGGTCAGGGAGTTGGATTCGTCTCAGGCGCTTGAACTGTTTAGTTACCATGCATTGCGAAAAGAAAAGCCCCCTGATGATTTCCTGGCCCTGTCCGAGCAAATTGTGTCTCTCACAGGTGGGCTACCGTTGGCTCTTGAAGTATTCGGCTCTTACCTATTATATCGGAGGAGAATAGAGGAATGGAGAGACGGTCTACAGAAACTGAAACATATTCGTCCCGGCAATCTTCAGGATGTGTTAAAGATAAGTTATGATGCTCTAGACGAGCAAGAGAAGTGTATCTTCCTAGACTTTGCATGTTTATTTGTGAAAATGAATATGAAGAGAGAAGATGCAATTGACATACTGAAGGGTTGTGGTTTTGATGGTGAGATAGCAATTGCTGATCTCACCGCAAAATCTCTCATGAAGGTTTATGAAAGCGGTATGCTGTGGATGCACGATCAAGTTAGAGACTTGGGAAGACAAATCGTTAGACATGAAAGCGTAGTAGATCCTGGCATGCGTAGTAGACTTTGGGATCGTGATGAAATCTTGAATGTCTTCAAGGATGATAAGGGAACAAGATGTATACAAGGGATAGTCTTAGACTTCGAATCATCGATAAGGATAGTGCGGGATCCAAGTGGTGAGAGAGTTTCGTGGGACAACTTTCGAAAAAGCCCCAGTTTTTGCTCTGCAATAACATACCTAAAGGAAAGGCACAAAGCTTATCTTCAAAATCAAGCAGAGAAAAAGAGAGAGGTTGTAATTTGCTCCAAACCCCTTGGAGCTATGGTCAATCTTAGACTTCTCCAAATTAATTTTGTGAATTTGGAAGGGAAGTTCAAGTTTCTTCCAGCTGAATTGAAGTGGTTACAATGGAAAGGTTGTCCTTTGAAATTTCTTCCTTCGGATTTTTCCCCTCGGCGACTTGCTGTCCTTGATCTTTCAAAGAGCAAACTTGTAAGTTTGTGGAGCGGGCATAACAAGGTGCCAGAGCAATTGATGTTCTTGATTCTACACGAATGCTCTTATCTAACTGCTATACCTGATTTATCTGGTAACTGCGCCTTGGAAAAGATTGTTCTTGAGCTATGTGTTAATCTCAATAAACTTCATGACTCGGTTGGGAATTTGAATACATTAGTCCACTTGAACCTGAGAGGTTGTTCGAACCTTATTGAACTTCCCAGTGATGTATCTGGCCTGAGAAAACTTGAGAACCTAATCCTCACCGGTTGCTCAAAATTGAAAAAACTGCCCAGCAACATGGACAGCATGGTATCATTGAAAGAACTTCTCCTTGACGAGACAGTGATAGAAAGTCTTCCTGAATCTATCTTCAAGCTTACGAAACTCGAAAAGCTCAGCTTGAATCCGATGCAAATTTTTGAAAGGGCTACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

587

Amino Acids

66.62

Weight (kDa)

8.45

Isoelectric Point (pI)

38.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 7 - 34 7.2e-06 NB-ARC domain
NB-ARC PF00931 61 - 145 3.9e-08 NB-ARC domain
WHD_ROQ1 PF23282 213 - 286 8.1e-14 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 468 - 579 8e-09 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1345
AccBSI CCGCTC 1 cut(s) 1332
AccI GTMKAC 3 cut(s) 565, 883, 943
AccII CGCG 1 cut(s) 126
AciI CCGC 5 cut(s) 126, 765, 795, 988, 1332
AclWI GGATC 5 cut(s) 15, 860, 900, 986, 999
AcsI RAATTY 6 cut(s) 45, 222, 1183, 1253, 1486, 1740
AcuI CTGAAG 2 cut(s) 584, 740
AgeI ACCGGT 1 cut(s) 1589
AhdI GACNNNNNGTC 1 cut(s) 1289
AjnI CCWGG 2 cut(s) 453, 868
AloI GAACNNNNNNTCC 2 cut(s) 1181, 1213
AluBI AGCT 7 cut(s) 1082, 1145, 1214, 1447, 1702, 1720, 1725
AluI AGCT 7 cut(s) 1082, 1145, 1214, 1447, 1702, 1720, 1725
Alw21I GWGCWC 1 cut(s) 42
Alw26I GTCTC 6 cut(s) 279, 396, 483, 552, 819, 1658
AlwI GGATC 5 cut(s) 15, 860, 900, 986, 999
AlwNI CAGNNNCTG 2 cut(s) 288, 574
AoxI GGCC 3 cut(s) 87, 456, 1558
ApoI RAATTY 6 cut(s) 45, 222, 1183, 1253, 1486, 1740
AseI ATTAAT 1 cut(s) 1173
AsiGI ACCGGT 1 cut(s) 1589
Asp700I GAANNNNTTC 2 cut(s) 914, 1680
AspLEI GCGC 2 cut(s) 400, 1424
AspS9I GGNCC 2 cut(s) 88, 457
AsuC2I CCSGG 1 cut(s) 591
AsuHPI GGTGA 4 cut(s) 752, 754, 1013, 1579
AsuII TTCGAA 3 cut(s) 966, 1027, 1526
BamHI GGATCC 1 cut(s) 991
BanI GGYRCC 1 cut(s) 1345
BauI CACGAG 1 cut(s) 164
BbsI GAAGAC 3 cut(s) 841, 907, 1673
Bbv12I GWGCWC 1 cut(s) 42
BccI CCATC 1 cut(s) 731
BciT130I CCWGG 2 cut(s) 455, 870
BcnI CCSGG 1 cut(s) 591
BcoDI GTCTC 6 cut(s) 279, 396, 483, 552, 819, 1658
BfaI CTAG 2 cut(s) 632, 659
BfmI CTRYAG 2 cut(s) 156, 566
BfoI RGCGCY 1 cut(s) 401
BisI GCNGC 1 cut(s) 127
BlpI GCTNAGC 1 cut(s) 1721
BlsI GCNGC 1 cut(s) 128
Bme1390I CCNGG 3 cut(s) 455, 591, 870
BmeRI GACNNNNNGTC 1 cut(s) 1289
BmgT120I GGNCC 2 cut(s) 88, 457
BmiI GGNNCC 2 cut(s) 993, 1347
BmrFI CCNGG 3 cut(s) 455, 591, 870
BmrI ACTGGG 2 cut(s) 1032, 1539
BmsI GCATC 5 cut(s) 130, 616, 694, 798, 1725
BmuI ACTGGG 2 cut(s) 1032, 1539
BpiI GAAGAC 3 cut(s) 841, 907, 1673
Bpu1102I GCTNAGC 1 cut(s) 1721
Bpu14I TTCGAA 3 cut(s) 966, 1027, 1526
BpuEI CTTGAG 2 cut(s) 1463, 1592
BpuMI CCSGG 1 cut(s) 591
Bsa29I ATCGAT 1 cut(s) 974
BsaJI CCNNGG 4 cut(s) 84, 1138, 1277, 1425
BsaWI WCCGGW 1 cut(s) 1589
BsaXI ACNNNNNCTCC 4 cut(s) 547, 577, 1134, 1164
Bse118I RCCGGY 1 cut(s) 1589
Bse1I ACTGG 3 cut(s) 293, 1038, 1545
Bse3DI GCAATG 1 cut(s) 422
BseBI CCWGG 2 cut(s) 455, 870
BseCI ATCGAT 1 cut(s) 974
BseDI CCNNGG 4 cut(s) 84, 1138, 1277, 1425
BseGI GGATG 4 cut(s) 244, 610, 813, 928
BseMI GCAATG 1 cut(s) 422
BseMII CTCAG 4 cut(s) 407, 1505, 1553, 1735
BseNI ACTGG 3 cut(s) 293, 1038, 1545
BseRI GAGGAG 1 cut(s) 553
BseYI CCCAGC 1 cut(s) 1616
Bsh1236I CGCG 1 cut(s) 126
BshFI GGCC 3 cut(s) 89, 458, 1560
BshNI GGYRCC 1 cut(s) 1345
BshTI ACCGGT 1 cut(s) 1589
BshVI ATCGAT 1 cut(s) 974
BsiHKAI GWGCWC 1 cut(s) 42
BsiSI CCGG 2 cut(s) 591, 1590
BslFI GGGAC 2 cut(s) 573, 1031
BsmAI GTCTC 6 cut(s) 279, 396, 483, 552, 819, 1658
BsmBI CGTCTC 2 cut(s) 396, 552
BsmFI GGGAC 2 cut(s) 573, 1031
BsmI GAATGC 1 cut(s) 1385
BsnI GGCC 3 cut(s) 89, 458, 1560
Bsp119I TTCGAA 3 cut(s) 966, 1027, 1526
Bsp1286I GDGCHC 1 cut(s) 42
Bsp143I GATC 8 cut(s) 20, 346, 757, 814, 865, 892, 991, 1297
Bsp1720I GCTNAGC 1 cut(s) 1721
BspACI CCGC 5 cut(s) 126, 765, 795, 988, 1332
BspANI GGCC 3 cut(s) 89, 458, 1560
BspCNI CTCAG 4 cut(s) 406, 1506, 1554, 1734
BspDI ATCGAT 1 cut(s) 974
BspFNI CGCG 1 cut(s) 126
BspHI TCATGA 2 cut(s) 777, 1471
BspLI GGNNCC 2 cut(s) 993, 1347
BspMAI CTGCAG 1 cut(s) 160
BspPI GGATC 5 cut(s) 15, 860, 900, 986, 999
BspT104I TTCGAA 3 cut(s) 966, 1027, 1526
BspT107I GGYRCC 1 cut(s) 1345
BsrBI CCGCTC 1 cut(s) 1332
BsrDI GCAATG 1 cut(s) 422
BsrFI RCCGGY 1 cut(s) 1589
BsrI ACTGG 3 cut(s) 293, 1038, 1545
BssAI RCCGGY 1 cut(s) 1589
BssECI CCNNGG 4 cut(s) 84, 1138, 1277, 1425
BssMI GATC 8 cut(s) 20, 346, 757, 814, 865, 892, 991, 1297
BssNAI GTATAC 1 cut(s) 944
BssSI CACGAG 1 cut(s) 164
BssT1I CCWWGG 3 cut(s) 84, 1138, 1425
Bst1107I GTATAC 1 cut(s) 944
Bst2BI CACGAG 1 cut(s) 164
Bst2UI CCWGG 2 cut(s) 455, 870
Bst4CI ACNGT 5 cut(s) 214, 408, 498, 563, 1669
Bst6I CTCTTC 1 cut(s) 689
BstBI TTCGAA 3 cut(s) 966, 1027, 1526
BstC8I GCNNGC 2 cut(s) 875, 1334
BstDEI CTNAG 7 cut(s) 263, 393, 958, 1157, 1514, 1562, 1721
BstF5I GGATG 4 cut(s) 244, 610, 813, 928
BstFNI CGCG 1 cut(s) 126
BstH2I RGCGCY 1 cut(s) 401
BstHHI GCGC 2 cut(s) 400, 1424
BstKTI GATC 8 cut(s) 23, 349, 760, 817, 868, 895, 994, 1300
BstMAI GTCTC 6 cut(s) 279, 396, 483, 552, 819, 1658
BstMBI GATC 8 cut(s) 20, 346, 757, 814, 865, 892, 991, 1297
BstMWI GCNNNNNNNGC 1 cut(s) 808
BstNI CCWGG 2 cut(s) 455, 870
BstNSI RCATGY 2 cut(s) 672, 877
BstSCI CCNGG 3 cut(s) 453, 589, 868
BstSFI CTRYAG 2 cut(s) 156, 566
BstUI CGCG 1 cut(s) 126
BstV2I GAAGAC 3 cut(s) 841, 907, 1673
BstX2I RGATCY 2 cut(s) 865, 991
BstXI CCANNNNNNTGG 1 cut(s) 1624
BstYI RGATCY 2 cut(s) 865, 991
BstZ17I GTATAC 1 cut(s) 944
Bsu15I ATCGAT 1 cut(s) 974
BsuRI GGCC 3 cut(s) 89, 458, 1560
BsuTUI ATCGAT 1 cut(s) 974
BtgZI GCGATG 1 cut(s) 107
BtsCI GGATG 4 cut(s) 244, 610, 813, 928
BtsIMutI CAGTG 2 cut(s) 1552, 1674
Cac8I GCNNGC 2 cut(s) 875, 1334
CaiI CAGNNNCTG 2 cut(s) 288, 574
CciI TCATGA 2 cut(s) 777, 1471
CfoI GCGC 2 cut(s) 400, 1424
Cfr10I RCCGGY 1 cut(s) 1589
Cfr13I GGNCC 2 cut(s) 88, 457
ClaI ATCGAT 1 cut(s) 974
CspAI ACCGGT 1 cut(s) 1589
DdeI CTNAG 7 cut(s) 263, 393, 958, 1157, 1514, 1562, 1721
DpnI GATC 8 cut(s) 22, 348, 759, 816, 867, 894, 993, 1299
DpnII GATC 8 cut(s) 20, 346, 757, 814, 865, 892, 991, 1297
DriI GACNNNNNGTC 1 cut(s) 1289
Eam1104I CTCTTC 1 cut(s) 689
Eam1105I GACNNNNNGTC 1 cut(s) 1289
EarI CTCTTC 1 cut(s) 689
Eco130I CCWWGG 3 cut(s) 84, 1138, 1425
Eco57I CTGAAG 2 cut(s) 584, 740
EcoO109I RGGNCCY 1 cut(s) 88
EcoRI GAATTC 2 cut(s) 45, 222
EcoRII CCWGG 2 cut(s) 453, 868
EcoT14I CCWWGG 3 cut(s) 84, 1138, 1425
EcoT22I ATGCAT 1 cut(s) 424
ErhI CCWWGG 3 cut(s) 84, 1138, 1425
Esp3I CGTCTC 2 cut(s) 396, 552
FalI AAGNNNNNCTT 6 cut(s) 638, 670, 1071, 1103, 1709, 1741
FaqI GGGAC 2 cut(s) 573, 1031
FauI CCCGC 2 cut(s) 981, 1325
FblI GTMKAC 3 cut(s) 565, 883, 943
Fnu4HI GCNGC 1 cut(s) 127
FokI GGATG 4 cut(s) 251, 617, 820, 935
Fsp4HI GCNGC 1 cut(s) 127
FspBI CTAG 2 cut(s) 632, 659
GlaI GCGC 2 cut(s) 399, 1423
GluI GCNGC 1 cut(s) 127
GsaI CCCAGC 1 cut(s) 1620
HaeII RGCGCY 1 cut(s) 401
HaeIII GGCC 3 cut(s) 89, 458, 1560
HapII CCGG 2 cut(s) 591, 1590
HhaI GCGC 2 cut(s) 400, 1424
Hin6I GCGC 2 cut(s) 398, 1422
HinP1I GCGC 2 cut(s) 398, 1422
HindIII AAGCTT 2 cut(s) 1080, 1700
HinfI GANTC 8 cut(s) 76, 309, 386, 968, 1369, 1475, 1688, 1729
HpaII CCGG 2 cut(s) 591, 1590
HphI GGTGA 4 cut(s) 752, 754, 1013, 1579
Hpy166II GTNNAC 5 cut(s) 358, 566, 884, 944, 1504
Hpy188I TCNGA 5 cut(s) 44, 466, 537, 1267, 1734
Hpy8I GTNNAC 5 cut(s) 358, 566, 884, 944, 1504
HpyAV CCTTC 5 cut(s) 293, 715, 775, 1184, 1272
HpyCH4III ACNGT 5 cut(s) 214, 408, 498, 563, 1669
HpyCH4V TGCA 7 cut(s) 158, 422, 668, 707, 811, 1052, 1738
HpyF10VI GCNNNNNNNGC 1 cut(s) 808
HpyF3I CTNAG 7 cut(s) 263, 393, 958, 1157, 1514, 1562, 1721
HspAI GCGC 2 cut(s) 398, 1422
Kzo9I GATC 8 cut(s) 20, 346, 757, 814, 865, 892, 991, 1297
LmnI GCTCC 3 cut(s) 1133, 1142, 1329
LweI GCATC 5 cut(s) 130, 616, 694, 798, 1725
MaeI CTAG 2 cut(s) 632, 659
MaeIII GTNAC 4 cut(s) 316, 413, 1227, 1415
MalI GATC 8 cut(s) 22, 348, 759, 816, 867, 894, 993, 1299
MbiI CCGCTC 1 cut(s) 1332
MboI GATC 8 cut(s) 20, 346, 757, 814, 865, 892, 991, 1297
MfeI CAATTG 3 cut(s) 708, 750, 1355
MflI RGATCY 2 cut(s) 865, 991
MhlI GDGCHC 1 cut(s) 42
MlyI GAGTC 2 cut(s) 70, 1469
MmeI TCCRAC 1 cut(s) 363
MnlI CCTC 8 cut(s) 160, 364, 531, 541, 1108, 1287, 1511, 1595
Mph1103I ATGCAT 1 cut(s) 424
MroXI GAANNNNTTC 2 cut(s) 914, 1680
MseI TTAA 4 cut(s) 96, 611, 1173, 1455
MspA1I CMGCKG 1 cut(s) 1214
MspI CCGG 2 cut(s) 591, 1590
MspR9I CCNGG 3 cut(s) 455, 591, 870
MunI CAATTG 3 cut(s) 708, 750, 1355
Mva1269I GAATGC 1 cut(s) 1385
MvaI CCWGG 2 cut(s) 455, 870
MvnI CGCG 1 cut(s) 126
MwoI GCNNNNNNNGC 1 cut(s) 808
NciI CCSGG 1 cut(s) 591
NdeII GATC 8 cut(s) 20, 346, 757, 814, 865, 892, 991, 1297
NlaIV GGNNCC 2 cut(s) 993, 1347
NmeAIII GCCGAG 1 cut(s) 1258
NsiI ATGCAT 1 cut(s) 424
NspI RCATGY 2 cut(s) 672, 877
NspV TTCGAA 3 cut(s) 966, 1027, 1526
PaeI GCATGC 1 cut(s) 877
PagI TCATGA 2 cut(s) 777, 1471
PctI GAATGC 1 cut(s) 1385
PdmI GAANNNNTTC 2 cut(s) 914, 1680
PfeI GAWTC 6 cut(s) 309, 386, 968, 1369, 1688, 1729
PinAI ACCGGT 1 cut(s) 1589
PkrI GCNGC 1 cut(s) 128
PleI GAGTC 2 cut(s) 70, 1469
PpsI GAGTC 2 cut(s) 70, 1469
PshBI ATTAAT 1 cut(s) 1173
Psp6I CCWGG 2 cut(s) 453, 868
PspFI CCCAGC 1 cut(s) 1616
PspGI CCWGG 2 cut(s) 453, 868
PspN4I GGNNCC 2 cut(s) 993, 1347
PspPI GGNCC 2 cut(s) 88, 457
PstI CTGCAG 1 cut(s) 160
PstNI CAGNNNCTG 2 cut(s) 288, 574
PsuI RGATCY 2 cut(s) 865, 991
PvuII CAGCTG 1 cut(s) 1214
SaqAI TTAA 4 cut(s) 96, 611, 1173, 1455
SatI GCNGC 1 cut(s) 127
Sau3AI GATC 8 cut(s) 20, 346, 757, 814, 865, 892, 991, 1297
Sau96I GGNCC 2 cut(s) 88, 457
SchI GAGTC 2 cut(s) 70, 1469
ScrFI CCNGG 3 cut(s) 455, 591, 870
SduI GDGCHC 1 cut(s) 42
SfaNI GCATC 5 cut(s) 130, 616, 694, 798, 1725
SfcI CTRYAG 2 cut(s) 156, 566
SfuI TTCGAA 3 cut(s) 966, 1027, 1526
SmlI CTYRAG 2 cut(s) 1442, 1571
SmoI CTYRAG 2 cut(s) 1442, 1571
SphI GCATGC 1 cut(s) 877
SsiI CCGC 5 cut(s) 126, 765, 795, 988, 1332
SspMI CTAG 2 cut(s) 632, 659
StyD4I CCNGG 3 cut(s) 453, 589, 868
StyI CCWWGG 3 cut(s) 84, 1138, 1425
TaaI ACNGT 5 cut(s) 214, 408, 498, 563, 1669
TaqI TCGA 8 cut(s) 19, 34, 117, 966, 974, 1027, 1526, 1713
TauI GCSGC 1 cut(s) 129
TfiI GAWTC 6 cut(s) 309, 386, 968, 1369, 1688, 1729
Tru1I TTAA 4 cut(s) 96, 611, 1173, 1455
Tru9I TTAA 4 cut(s) 96, 611, 1173, 1455
TscAI CASTG 2 cut(s) 1552, 1674
TspRI CASTG 2 cut(s) 1552, 1674
VspI ATTAAT 1 cut(s) 1173
XapI RAATTY 6 cut(s) 45, 222, 1183, 1253, 1486, 1740
XbaI TCTAGA 1 cut(s) 631
XceI RCATGY 2 cut(s) 672, 877
XmiI GTMKAC 3 cut(s) 565, 883, 943
XmnI GAANNNNTTC 2 cut(s) 914, 1680
XspI CTAG 2 cut(s) 632, 659
Zsp2I ATGCAT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.