Rorug04G0358300

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001217
Physical Location & Seq
Reverse (-)
53117934 .. 53120446
2513 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0358300.1

Sequence Viewer

Length: 1224 bp
ATGAAGGGTGGTAGTGGTGGTTCTACTCAGAATCTGAGCACTGTAAAATTTTTTCCAAAGCTCATCAATGTCCAACTCCATCACCTCATCAGTGTCCTCTCTTATTTCCTCTTATTTGGTGTTGGAATAACCCTCGGGATGATCCTCACCTTCTATCTCAAAGACTTTTCTTTCAGTCTCCAACTGGCCCATTTCTCCTTCTCAACCTCATCATCTTCATCTAATGGAAACACGATACTGCCGAGTGTGACAGCTGCGCAAACCAATTTGAGTCACCCCCAGCACATCGGATTGGAGAAATTTCTGAAACCCCCTAAGATTTTGCACGACATGAATGATGAAGAATTGCTATGGAGAGCTTCTATGGCTCCACGGAGATCCGGATATCCATTCCATCGAGTTCCGAAAGTTGCCTTCTTGTTCTTGACAAAGGGACCAGTTTTTCTGGCCCCATTGTGGGAGAAGTTCTTCGAAGGGCATCAAGGTTTGTACTCGATATATGTGCACTCGAATCCGTCTTACAATGGATCGTACCCCGAAAGTCCGGTGTTTCAAGGCAGAAGAATTCCTAGTCAGGAAGTGGAATGGGGAAATGTGAACATGATTGAGGCTGAGCGCCGCCTATTGGCAAATGCCCTTCTTGATATCTCAAACGATCGCTTTGCTCTTCTCTCGGAGTCATGCATCCCCCTCCACAACTTCAGCACCATCTACCACTTCCTTGTAAATTCGAAGCAGACATTTGTTCAGGTCTATGACGATCCAAGTAGTGTTGGGCGCGGGAGATTCAATTCACACATGTATCCGCAAATCTCATTGTCACAATGGAGGAAAGGATCTCAGTGGTTCCAGATAGATAGAGACATTGCCACTGAAGTTGTCGCAGACAAAAAGTACTTCCCACTTTTCCAGAAGTACTGCAGGGGCTCATGCTACGCAGACGAGCATTACTTCCCAACATTTGTTCATATGAAATTTTCTGCAAAGAATTCAAATAAAACCTTGACTTGGTATGACTTTTCGAGGGGTGGACCGCACCCTGCCCGGTTCACCAGAACAGACGTGACGGTTGATCTGCTGAACAGATTCAGGAATGGTACCAAATGTTTTGATTATCTAATGACAGGCAAAGCTTGTCCTCTCTTGTTTGCTAGGAAGTTCACTCCTGGCACTTTGGAGAGACTGCTGCGGTTTGCACCAAAATTGATGCAGTTCAACAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

407

Amino Acids

46.67

Weight (kDa)

9.6

Isoelectric Point (pI)

39.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 137 - 362 2.9e-77 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 258
Acc65I GGTACC 1 cut(s) 1097
AccB1I GGYRCC 1 cut(s) 1097
AccII CGCG 1 cut(s) 780
AccIII TCCGGA 1 cut(s) 380
AciI CCGC 5 cut(s) 619, 780, 806, 1034, 1189
AclWI GGATC 5 cut(s) 136, 372, 535, 755, 844
AcsI RAATTY 6 cut(s) 47, 299, 564, 727, 974, 988
AcuI CTGAAG 2 cut(s) 685, 894
AfaI GTAC 5 cut(s) 491, 533, 896, 917, 1099
AfiI CCNNNNNNNGG 4 cut(s) 456, 457, 625, 1008
AflIII ACRYGT 1 cut(s) 798
AgsI TTSAA 4 cut(s) 554, 790, 993, 1216
AjiI CACGTC 1 cut(s) 1063
AjnI CCWGG 1 cut(s) 1165
AluBI AGCT 4 cut(s) 61, 254, 359, 1133
AluI AGCT 4 cut(s) 61, 254, 359, 1133
Alw21I GWGCWC 2 cut(s) 41, 507
Alw26I GTCTC 3 cut(s) 182, 855, 1174
Alw44I GTGCAC 1 cut(s) 503
AlwI GGATC 5 cut(s) 136, 372, 535, 755, 844
AlwNI CAGNNNCTG 1 cut(s) 34
Ama87I CYCGRG 1 cut(s) 134
Aor13HI TCCGGA 1 cut(s) 380
AoxI GGCC 2 cut(s) 186, 447
ApaLI GTGCAC 1 cut(s) 503
ApeKI GCWGC 2 cut(s) 254, 1186
ApoI RAATTY 6 cut(s) 47, 299, 564, 727, 974, 988
Asp700I GAANNNNTTC 2 cut(s) 467, 1085
Asp718I GGTACC 1 cut(s) 1097
AspLEI GCGC 3 cut(s) 259, 618, 780
AspS9I GGNCC 4 cut(s) 187, 434, 448, 1031
AsuC2I CCSGG 1 cut(s) 1045
AsuHPI GGTGA 4 cut(s) 74, 139, 266, 1042
AsuII TTCGAA 2 cut(s) 471, 731
AvaI CYCGRG 1 cut(s) 134
AvaII GGWCC 2 cut(s) 434, 1031
BaeGI GKGCMC 1 cut(s) 507
BaeI ACNNNNGTAYC 2 cut(s) 785, 818
BanI GGYRCC 1 cut(s) 1097
BanII GRGCYC 1 cut(s) 929
Bbv12I GWGCWC 2 cut(s) 41, 507
BbvI GCAGC 2 cut(s) 241, 1173
BccI CCATC 3 cut(s) 87, 402, 716
BcgI CGANNNNNNTGC 4 cut(s) 484, 518, 644, 678
BciT130I CCWGG 1 cut(s) 1167
BciVI GTATCC 1 cut(s) 813
BcnI CCSGG 1 cut(s) 1045
BcoDI GTCTC 3 cut(s) 182, 855, 1174
BfaI CTAG 2 cut(s) 570, 1152
BfmI CTRYAG 1 cut(s) 919
BfoI RGCGCY 1 cut(s) 619
BfuI GTATCC 1 cut(s) 813
BisI GCNGC 3 cut(s) 255, 619, 1187
BlpI GCTNAGC 1 cut(s) 612
BlsI GCNGC 3 cut(s) 256, 620, 1188
BmcAI AGTACT 2 cut(s) 896, 917
Bme1390I CCNGG 2 cut(s) 1045, 1167
Bme18I GGWCC 2 cut(s) 434, 1031
BmeT110I CYCGRG 1 cut(s) 134
BmgBI CACGTC 1 cut(s) 1063
BmgT120I GGNCC 4 cut(s) 187, 434, 448, 1031
BmiI GGNNCC 5 cut(s) 369, 435, 450, 848, 1099
BmrFI CCNGG 2 cut(s) 1045, 1167
BmsI GCATC 3 cut(s) 487, 693, 1197
Bpu1102I GCTNAGC 1 cut(s) 612
Bpu14I TTCGAA 2 cut(s) 471, 731
BpuMI CCSGG 1 cut(s) 1045
BsaJI CCNNGG 2 cut(s) 133, 371
BsaWI WCCGGW 2 cut(s) 380, 544
Bsc4I CCNNNNNNNGG 4 cut(s) 456, 457, 625, 1008
Bse1I ACTGG 2 cut(s) 189, 437
Bse3DI GCAATG 1 cut(s) 864
BseAI TCCGGA 1 cut(s) 380
BseBI CCWGG 1 cut(s) 1167
BseDI CCNNGG 2 cut(s) 133, 371
BseGI GGATG 2 cut(s) 144, 684
BseLI CCNNNNNNNGG 4 cut(s) 456, 457, 625, 1008
BseMI GCAATG 1 cut(s) 864
BseMII CTCAG 4 cut(s) 26, 41, 603, 854
BseNI ACTGG 2 cut(s) 189, 437
BseSI GKGCMC 1 cut(s) 507
BseXI GCAGC 2 cut(s) 241, 1173
BseYI CCCAGC 1 cut(s) 279
Bsh1236I CGCG 1 cut(s) 780
Bsh1285I CGRYCG 1 cut(s) 658
BshFI GGCC 2 cut(s) 188, 449
BshNI GGYRCC 1 cut(s) 1097
BsiEI CGRYCG 1 cut(s) 658
BsiHKAI GWGCWC 2 cut(s) 41, 507
BsiHKCI CYCGRG 1 cut(s) 134
BsiSI CCGG 3 cut(s) 381, 545, 1045
BslFI GGGAC 1 cut(s) 447
BslI CCNNNNNNNGG 4 cut(s) 456, 457, 625, 1008
BsmAI GTCTC 3 cut(s) 182, 855, 1174
BsmFI GGGAC 1 cut(s) 447
BsnI GGCC 2 cut(s) 188, 449
BsoBI CYCGRG 1 cut(s) 134
Bsp119I TTCGAA 2 cut(s) 471, 731
Bsp1286I GDGCHC 3 cut(s) 41, 507, 929
Bsp13I TCCGGA 1 cut(s) 380
Bsp143I GATC 7 cut(s) 141, 377, 527, 655, 760, 836, 1072
Bsp1720I GCTNAGC 1 cut(s) 612
BspACI CCGC 5 cut(s) 619, 780, 806, 1034, 1189
BspANI GGCC 2 cut(s) 188, 449
BspCNI CTCAG 4 cut(s) 27, 40, 604, 853
BspEI TCCGGA 1 cut(s) 380
BspFNI CGCG 1 cut(s) 780
BspLI GGNNCC 5 cut(s) 369, 435, 450, 848, 1099
BspMAI CTGCAG 1 cut(s) 923
BspPI GGATC 5 cut(s) 136, 372, 535, 755, 844
BspQI GCTCTTC 1 cut(s) 672
BspT104I TTCGAA 2 cut(s) 471, 731
BspT107I GGYRCC 1 cut(s) 1097
BsrDI GCAATG 1 cut(s) 864
BsrI ACTGG 2 cut(s) 189, 437
BssECI CCNNGG 2 cut(s) 133, 371
BssMI GATC 7 cut(s) 141, 377, 527, 655, 760, 836, 1072
Bst2UI CCWGG 1 cut(s) 1167
Bst4CI ACNGT 2 cut(s) 43, 1069
Bst6I CTCTTC 1 cut(s) 672
BstBI TTCGAA 2 cut(s) 471, 731
BstDEI CTNAG 5 cut(s) 27, 35, 315, 612, 840
BstDSI CCRYGG 1 cut(s) 371
BstF5I GGATG 2 cut(s) 144, 684
BstFNI CGCG 1 cut(s) 780
BstH2I RGCGCY 1 cut(s) 619
BstHHI GCGC 3 cut(s) 259, 618, 780
BstKTI GATC 7 cut(s) 144, 380, 530, 658, 763, 839, 1075
BstMAI GTCTC 3 cut(s) 182, 855, 1174
BstMBI GATC 7 cut(s) 141, 377, 527, 655, 760, 836, 1072
BstMCI CGRYCG 1 cut(s) 658
BstMWI GCNNNNNNNGC 1 cut(s) 365
BstNI CCWGG 1 cut(s) 1167
BstNSI RCATGY 1 cut(s) 802
BstSCI CCNGG 2 cut(s) 1043, 1165
BstSFI CTRYAG 1 cut(s) 919
BstSLI GKGCMC 1 cut(s) 507
BstUI CGCG 1 cut(s) 780
BstV1I GCAGC 2 cut(s) 241, 1173
BstX2I RGATCY 2 cut(s) 377, 836
BstYI RGATCY 2 cut(s) 377, 836
BsuI GTATCC 1 cut(s) 813
BsuRI GGCC 2 cut(s) 188, 449
BtgI CCRYGG 1 cut(s) 371
BtrI CACGTC 1 cut(s) 1063
BtsCI GGATG 2 cut(s) 144, 684
BtsIMutI CAGTG 4 cut(s) 39, 97, 848, 870
CaiI CAGNNNCTG 1 cut(s) 34
CfoI GCGC 3 cut(s) 259, 618, 780
Cfr13I GGNCC 4 cut(s) 187, 434, 448, 1031
Csp6I GTAC 5 cut(s) 490, 532, 895, 916, 1098
CspCI CAANNNNNGTGG 2 cut(s) 859, 894
CviAII CATG 5 cut(s) 331, 601, 681, 799, 930
CviJI RGCY 9 cut(s) 61, 188, 254, 359, 368, 449, 611, 927, 1133
CviKI_1 RGCY 9 cut(s) 61, 188, 254, 359, 368, 449, 611, 927, 1133
CviQI GTAC 5 cut(s) 490, 532, 895, 916, 1098
DdeI CTNAG 5 cut(s) 27, 35, 315, 612, 840
DpnI GATC 7 cut(s) 143, 379, 529, 657, 762, 838, 1074
DpnII GATC 7 cut(s) 141, 377, 527, 655, 760, 836, 1072
Eam1104I CTCTTC 1 cut(s) 672
EarI CTCTTC 1 cut(s) 672
Eco24I GRGCYC 1 cut(s) 929
Eco32I GATATC 2 cut(s) 386, 646
Eco47I GGWCC 2 cut(s) 434, 1031
Eco57I CTGAAG 2 cut(s) 685, 894
Eco88I CYCGRG 1 cut(s) 134
EcoRI GAATTC 2 cut(s) 564, 988
EcoRII CCWGG 1 cut(s) 1165
EcoRV GATATC 2 cut(s) 386, 646
EcoT22I ATGCAT 1 cut(s) 686
EcoT38I GRGCYC 1 cut(s) 929
FaeI CATG 5 cut(s) 334, 604, 684, 802, 933
FaqI GGGAC 1 cut(s) 447
FatI CATG 5 cut(s) 330, 600, 680, 798, 929
FauI CCCGC 1 cut(s) 773
FauNDI CATATG 1 cut(s) 969
Fnu4HI GCNGC 3 cut(s) 255, 619, 1187
FokI GGATG 2 cut(s) 151, 671
FriOI GRGCYC 1 cut(s) 929
Fsp4HI GCNGC 3 cut(s) 255, 619, 1187
FspBI CTAG 2 cut(s) 570, 1152
FspI TGCGCA 1 cut(s) 258
GlaI GCGC 3 cut(s) 258, 617, 779
GluI GCNGC 3 cut(s) 255, 619, 1187
GsaI CCCAGC 1 cut(s) 283
HaeII RGCGCY 1 cut(s) 619
HaeIII GGCC 2 cut(s) 188, 449
HapII CCGG 3 cut(s) 381, 545, 1045
HhaI GCGC 3 cut(s) 259, 618, 780
Hin1II CATG 5 cut(s) 334, 604, 684, 802, 933
Hin6I GCGC 3 cut(s) 257, 616, 778
HinP1I GCGC 3 cut(s) 257, 616, 778
HindIII AAGCTT 1 cut(s) 1131
HinfI GANTC 6 cut(s) 31, 271, 511, 677, 786, 1086
HpaII CCGG 3 cut(s) 381, 545, 1045
HphI GGTGA 4 cut(s) 74, 139, 266, 1042
Hpy166II GTNNAC 5 cut(s) 505, 598, 1031, 1050, 1161
Hpy188I TCNGA 6 cut(s) 30, 36, 290, 306, 405, 676
Hpy188III TCNNGA 8 cut(s) 136, 381, 424, 575, 641, 850, 910, 1090
Hpy8I GTNNAC 5 cut(s) 505, 598, 1031, 1050, 1161
HpyAV CCTTC 5 cut(s) 160, 208, 424, 467, 647
HpyCH4III ACNGT 2 cut(s) 43, 1069
HpyCH4IV ACGT 1 cut(s) 1062
HpyCH4V TGCA 7 cut(s) 325, 505, 684, 921, 983, 1196, 1210
HpyF10VI GCNNNNNNNGC 1 cut(s) 365
HpyF3I CTNAG 5 cut(s) 27, 35, 315, 612, 840
HpySE526I ACGT 1 cut(s) 1062
Hsp92II CATG 5 cut(s) 334, 604, 684, 802, 933
HspAI GCGC 3 cut(s) 257, 616, 778
Kpn2I TCCGGA 1 cut(s) 380
KpnI GGTACC 1 cut(s) 1101
Kzo9I GATC 7 cut(s) 141, 377, 527, 655, 760, 836, 1072
LguI GCTCTTC 1 cut(s) 672
LmnI GCTCC 1 cut(s) 373
Lsp1109I GCAGC 2 cut(s) 241, 1173
LweI GCATC 3 cut(s) 487, 693, 1197
MaeI CTAG 2 cut(s) 570, 1152
MaeII ACGT 1 cut(s) 1062
MaeIII GTNAC 4 cut(s) 247, 272, 819, 1063
MalI GATC 7 cut(s) 143, 379, 529, 657, 762, 838, 1074
MboI GATC 7 cut(s) 141, 377, 527, 655, 760, 836, 1072
MboII GAAGA 5 cut(s) 207, 353, 460, 573, 659
MflI RGATCY 2 cut(s) 377, 836
MhlI GDGCHC 3 cut(s) 41, 507, 929
MlyI GAGTC 2 cut(s) 280, 686
MmeI TCCRAC 3 cut(s) 97, 103, 205
Mph1103I ATGCAT 1 cut(s) 686
MroI TCCGGA 1 cut(s) 380
MroXI GAANNNNTTC 2 cut(s) 467, 1085
MspA1I CMGCKG 1 cut(s) 254
MspI CCGG 3 cut(s) 381, 545, 1045
MspR9I CCNGG 2 cut(s) 1045, 1167
MvaI CCWGG 1 cut(s) 1167
MvnI CGCG 1 cut(s) 780
MwoI GCNNNNNNNGC 1 cut(s) 365
NciI CCSGG 1 cut(s) 1045
NdeI CATATG 1 cut(s) 969
NdeII GATC 7 cut(s) 141, 377, 527, 655, 760, 836, 1072
NlaIII CATG 5 cut(s) 334, 604, 684, 802, 933
NlaIV GGNNCC 5 cut(s) 369, 435, 450, 848, 1099
NmeAIII GCCGAG 1 cut(s) 267
NmuCI GTSAC 4 cut(s) 247, 272, 819, 1063
NsbI TGCGCA 1 cut(s) 258
NsiI ATGCAT 1 cut(s) 686
NspI RCATGY 1 cut(s) 802
NspV TTCGAA 2 cut(s) 471, 731
PciI ACATGT 1 cut(s) 798
PciSI GCTCTTC 1 cut(s) 672
PcsI WCGNNNNNNNCGW 1 cut(s) 239
PdmI GAANNNNTTC 2 cut(s) 467, 1085
PfeI GAWTC 4 cut(s) 31, 511, 786, 1086
PkrI GCNGC 3 cut(s) 256, 620, 1188
Ple19I CGATCG 1 cut(s) 658
PleI GAGTC 2 cut(s) 279, 685
PpsI GAGTC 2 cut(s) 279, 685
PscI ACATGT 1 cut(s) 798
Psp6I CCWGG 1 cut(s) 1165
PspFI CCCAGC 1 cut(s) 279
PspGI CCWGG 1 cut(s) 1165
PspN4I GGNNCC 5 cut(s) 369, 435, 450, 848, 1099
PspPI GGNCC 4 cut(s) 187, 434, 448, 1031
PsrI GAACNNNNNNTAC 1 cut(s) 36
PstI CTGCAG 1 cut(s) 923
PstNI CAGNNNCTG 1 cut(s) 34
PsuI RGATCY 2 cut(s) 377, 836
PvuI CGATCG 1 cut(s) 658
PvuII CAGCTG 1 cut(s) 254
RsaI GTAC 5 cut(s) 491, 533, 896, 917, 1099
RsaNI GTAC 5 cut(s) 490, 532, 895, 916, 1098
SapI GCTCTTC 1 cut(s) 672
SatI GCNGC 3 cut(s) 255, 619, 1187
Sau3AI GATC 7 cut(s) 141, 377, 527, 655, 760, 836, 1072
Sau96I GGNCC 4 cut(s) 187, 434, 448, 1031
ScaI AGTACT 2 cut(s) 896, 917
SchI GAGTC 2 cut(s) 280, 686
ScrFI CCNGG 2 cut(s) 1045, 1167
SduI GDGCHC 3 cut(s) 41, 507, 929
SfaNI GCATC 3 cut(s) 487, 693, 1197
SfcI CTRYAG 1 cut(s) 919
SfuI TTCGAA 2 cut(s) 471, 731
SinI GGWCC 2 cut(s) 434, 1031
SsiI CCGC 5 cut(s) 619, 780, 806, 1034, 1189
SspMI CTAG 2 cut(s) 570, 1152
StyD4I CCNGG 2 cut(s) 1043, 1165
TaaI ACNGT 2 cut(s) 43, 1069
TaiI ACGT 1 cut(s) 1065
TaqI TCGA 6 cut(s) 397, 471, 494, 509, 731, 1022
TatI WGTACW 3 cut(s) 489, 894, 915
TauI GCSGC 1 cut(s) 621
TfiI GAWTC 4 cut(s) 31, 511, 786, 1086
TscAI CASTG 4 cut(s) 46, 97, 848, 877
TseFI GTSAC 4 cut(s) 247, 272, 819, 1063
TseI GCWGC 2 cut(s) 254, 1186
Tsp45I GTSAC 4 cut(s) 247, 272, 819, 1063
TspDTI ATGAA 6 cut(s) 17, 207, 347, 354, 956, 986
TspGWI ACGGA 2 cut(s) 388, 504
TspRI CASTG 4 cut(s) 46, 97, 848, 877
VneI GTGCAC 1 cut(s) 503
VpaK11BI GGWCC 2 cut(s) 434, 1031
XapI RAATTY 6 cut(s) 47, 299, 564, 727, 974, 988
XceI RCATGY 1 cut(s) 802
XmnI GAANNNNTTC 2 cut(s) 467, 1085
XspI CTAG 2 cut(s) 570, 1152
ZrmI AGTACT 2 cut(s) 896, 917
Zsp2I ATGCAT 1 cut(s) 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.