MD16G1082700.v1.1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001217
Physical Location & Seq
Reverse (-)
5790493 .. 5795267
4775 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1082700.v1.1.491

Sequence Viewer

Length: 2973 bp
ATGGCGGGAGAAGACGACGACGGCGACGCCGTTTCGTCAACCCCGGGTGGCTTCCGGCTACGCTGGGACGTGTTCCTGAGTTTCAGAGGAGAAGACACCCGCGCAACAATCACGAAGAGCTTGTACGAGGCCCTCAAAAGCCGCGGCGTCCGAGTCTTCTTAGACGACGACGGTTTGGACCGCGGGGACGACATCGCCCCAAGCCTGCTGGAAGCGATCGACGACTCGGCGGCCGCAATCGTCGTCTTGTCGCGGAGATACGCCGATTCGAGGTGGTGCTTGGAGGAGCTGGCTAAGATATGCGAGAGCAGCAGGAGGCGTCTGATTCTGCCTGTGTTCTACCAAGTGGATCCCTCCGACGTCCGACGACAGAGGGGCCCGTTTTCCGAACATTTTTCAGCTCACGAACTGCAGTATGGAAACGCCGTCGTGTCGAGATGGAGGAGCGCCATGGCCAAAGTCGGCGGCAAAGCCGGTTACGTTTGCGAACTCCTCCAGGAAGCAGAGGTGGTTCAGGGGTTGGTCAAAAAGGTTTTGAATGAAATAAGGAAAACCCCGGTAGGTCTTGCTTCCTACACAGTGGGACTTGATTCTCGAGTTGAAGATGTGATGAGGTTGTTAGATGTTCGATCCAATGGCGTTCGAGTGCTCGGAATTCATGGCATGGGTGGTGTTGGCAAGACGACTCTTGCCAAGGCCCTTTTTAACAGACTTGTTGCTTACTTTGAGCATCGCGGCTTCATCTCAAATGTTAGAGAAACTTCTGCAGGACACGAGGGGTTAGTATCGCTTCAAAACAGACTTATCGGTAGTCTTTCTACTGCGAAGATGCCGGTAAATGAACTTAACGCTGGTATTTCTGCAATAAAAGCGAGTGTGTACGAGAAGCGAGTTCTTATTGTTTTGGATGATGTTGATAATGTAGAGCAACTAAGTGCTTTAGTTGGCAACAGAGACTGGTTTTATGAAGGAAGCAGAATCATTGTTACCACAAGAGATAGAAGTGTCTTGCCCGATCATCTTGTGAACAAGTTGTATGAGGTCAGGGAGTTGGATTCGTCTCAGGCGCTTGAACTGTTTAGTTACCATGCACTGCGAAAAGAAAAGCCCCCTGAGGATTTCCTGGCCCTGTCCGAGCAAATTGTGTCTCTCACAGGTGGGCTACCGTTGGCTCTTGAAGTTTTCGGCTCTTACCTATTTTATCAGAGGAGAATAGAGGAATGGAGAGACGTTCTGCAGAAACTGAAACAGATTCGTCCCGGCAATCTTCAGGATGTGTTAAAGATAAGTTATGATGCTCTAGACGAGCAAGAGAAGTGTATCTTCCTAGACTTTGCATGTTTATTTGTGAAAATGAATATGAAGAGAGAAGATGCAATTGACATACTGAAGGGTTGTGGTTTTGATGGTGAGATAGCAATTGCTGACCTCACCGCAAAATCTCTCATGAAGGTTTATGAAGACGGCATGCTTTGGATGCACGATCAAGTTAGAGACATGGGAAGACAAATCGTTGTACATGAAAGCGTTGTAGATCCTGGCATGCGTAGTAGACTTTGGGATCGTGATGAAATCTTGAATGTCTTCAAGGATGATAAGGGAACAAGATGTATACAAGGGATAGTCTTAGACTTCGAATCATCGATAAGGAGGGTGTGGGATCCAAGTGGTGAGAGAGTTTCGTGGGACAACTTTCGAAAAAGCCCCAGTTTTTGCTCTGCAGTAACATACCTAAAGGAAAGGCACAAAGCTTATCTTCAAAATCAAGCAGAGAAAAAGAGAAAGGTTGTAATTTGCTCCAAACCCCTTGGAGCAATGGTCAATCTTAGACTTCTCCAAATTAATTTTGTGAATTTGGAAGGGAAGTTCAAGTTTCTTCCAGCTGAGTTGAAGTGGTTACAATGGAAAGGTTGTCCTATGAAATTTCTTCCTTCTGATTTTTCCCCTCGGCGACTTGCTGTCCTTGATCTTTCAAATAGCAAACTTGTAAGTTTGTGGCGCGGGCGTAACAAGGTGCCAGAGCAATTGATGTTCTTGATTCTACACAAATGCTCTTATCTAACTGCTATACCTGATTTATCTGGTAACCGCGCCTTGGAAAAGATTGTTCTTGAGCTATGTGTTAATCTCAATAAACTTCATGACTCGGTTGGGAATTTGAATACATTAGTCCACTTGAACCTGAGAGGTTGTTCAAACCTTATTGAACTTCCCAGTGATGTATCTGGCCTGAGAAAACTTGAGAACCTATTCCTCACCGGTTGCTCAAAATTGAAAAAACTGCCCAGCAACATGGACAGCATGGTATCATTGAAAGAACTTCTCCTTGACGAGACAGTGATAGAGAGTCTTCCTGAATCTATCTTCAAGCTTACGAAACTCGAAAGGCTCAGCTTGAACCGATGCAAATTTTTGAAAGGGCTACCTGAGTTGATAGGAAAGTTGTGTTCTCTCAAAGAAATATCCCTTAATGAATCAGCTTTAGAAAAACTACCCGATTCATTTGGATCTTTGGCAAACCTTGAGAGACTAAGTTTGCTCTGGTGTGAATCACTGACCACCATTCCAGATTCCATTGGAAACCTGAATTCGTTAGTGGAATTTCTTACTTATGGTAGTCCTATCAAAGAACTGCCTGCGTCCATTGGTTCATTGTCAAACTTGAGGGAGTTATCGGTGGGGCGCGGCGAGTTTATGAGAGTATTGCCTGATTCAGTTGGAGGTTTAAATTCTCTAGTTGTGCTACAAATAGATGAGACATTAATCACAAATCTCCCACATGAAATCGGTGCCTTGAAAACACTTGAGAAGCTTGAGATGAGGAAATGTGGATTTCTTAGATCATTACCGCAGTCAATCGGAAAGCATGAGGGGACTAACTGCTATAGTCATAACCGATGCTACTATCACAGAGTTGCCAGAATCAGTAGGGATGTTGGAAAATCTTACCGTGTTGCGCTTGAACGGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

991

Amino Acids

111.57

Weight (kDa)

8.31

Isoelectric Point (pI)

40.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 22 - 184 8.3e-39 TIR domain
TIR_2 PF13676 24 - 118 3e-17 TIR domain
NB-ARC PF00931 199 - 368 5.8e-19 NB-ARC domain
NACHT PF05729 216 - 361 3.1e-06 NACHT domain
WHD_ROQ1 PF23282 436 - 508 5.2e-13 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 689 - 801 1.2e-08 Leucine-rich repeat region
LRR_14 PF23598 806 - 868 2.3e-07 Leucine-rich repeat region
LRR_14 PF23598 873 - 941 2.7e-09 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 363
AccB1I GGYRCC 2 cut(s) 2014, 2789
AccI GTMKAC 2 cut(s) 1552, 1612
AccII CGCG 8 cut(s) 102, 144, 183, 253, 735, 2001, 2091, 2685
AclWI GGATC 8 cut(s) 344, 357, 624, 1529, 1569, 1655, 1668, 2515
AcoI YGGCCR 2 cut(s) 231, 453
AcsI RAATTY 8 cut(s) 654, 1852, 1922, 2155, 2408, 2587, 2600, 2728
AcuI CTGAAG 2 cut(s) 1253, 1409
AcyI GRCGYC 4 cut(s) 27, 147, 319, 360
AfaI GTAC 3 cut(s) 125, 881, 1518
AfiI CCNNNNNNNGG 2 cut(s) 270, 2095
AflIII ACRYGT 1 cut(s) 69
AgeI ACCGGT 1 cut(s) 2258
AhdI GACNNNNNGTC 1 cut(s) 1958
AjiI CACGTC 1 cut(s) 70
AjnI CCWGG 3 cut(s) 495, 1122, 1537
AloI GAACNNNNNNTCC 2 cut(s) 1850, 1882
Alw21I GWGCWC 1 cut(s) 651
Alw26I GTCTC 8 cut(s) 948, 1065, 1152, 1221, 1488, 2327, 2521, 2750
AlwI GGATC 8 cut(s) 344, 357, 624, 1529, 1569, 1655, 1668, 2515
AlwNI CAGNNNCTG 2 cut(s) 957, 1243
Ama87I CYCGRG 2 cut(s) 43, 594
AoxI GGCC 7 cut(s) 129, 231, 376, 453, 696, 1125, 2227
ApaI GGGCCC 1 cut(s) 380
ApeKI GCWGC 1 cut(s) 309
ApoI RAATTY 8 cut(s) 654, 1852, 1922, 2155, 2408, 2587, 2600, 2728
AseI ATTAAT 2 cut(s) 1842, 2762
AsiGI ACCGGT 1 cut(s) 2258
Asp700I GAANNNNTTC 2 cut(s) 1583, 2249
AspLEI GCGC 7 cut(s) 104, 449, 1069, 2001, 2093, 2685, 2959
AspS9I GGNCC 6 cut(s) 130, 178, 376, 377, 697, 1126
AsuC2I CCSGG 4 cut(s) 44, 45, 557, 1260
AsuHPI GGTGA 4 cut(s) 1421, 1423, 1682, 2248
AsuII TTCGAA 2 cut(s) 1635, 1696
AvaI CYCGRG 2 cut(s) 43, 594
AvaII GGWCC 1 cut(s) 178
AxyI CCTNAGG 1 cut(s) 1113
BaeGI GKGCMC 1 cut(s) 380
BalI TGGCCA 1 cut(s) 455
BamHI GGATCC 2 cut(s) 349, 1660
BanI GGYRCC 2 cut(s) 2014, 2789
BanII GRGCYC 1 cut(s) 380
BarI GAAGNNNNNNTAC 2 cut(s) 107, 139
BauI CACGAG 1 cut(s) 773
BbsI GAAGAC 7 cut(s) 18, 99, 148, 1467, 1510, 1576, 2342
Bbv12I GWGCWC 1 cut(s) 651
BbvI GCAGC 1 cut(s) 321
BccI CCATC 2 cut(s) 432, 1400
BceAI ACGGC 4 cut(s) 14, 37, 410, 1480
BciT130I CCWGG 3 cut(s) 497, 1124, 1539
BcnI CCSGG 4 cut(s) 44, 45, 557, 1260
BcoDI GTCTC 8 cut(s) 948, 1065, 1152, 1221, 1488, 2327, 2521, 2750
BfaI CTAG 3 cut(s) 1301, 1328, 2735
BfmI CTRYAG 5 cut(s) 410, 765, 1235, 1719, 2884
BfoI RGCGCY 2 cut(s) 450, 1070
BisI GCNGC 8 cut(s) 142, 145, 231, 234, 310, 466, 736, 2686
BlpI GCTNAGC 1 cut(s) 2390
BlsI GCNGC 8 cut(s) 143, 146, 232, 235, 311, 467, 737, 2687
Bme1390I CCNGG 7 cut(s) 44, 45, 497, 557, 1124, 1260, 1539
Bme18I GGWCC 1 cut(s) 178
BmeRI GACNNNNNGTC 1 cut(s) 1958
BmeT110I CYCGRG 2 cut(s) 43, 594
BmgBI CACGTC 1 cut(s) 70
BmgT120I GGNCC 6 cut(s) 130, 178, 376, 377, 697, 1126
BmiI GGNNCC 6 cut(s) 351, 377, 378, 1662, 2016, 2791
BmrFI CCNGG 7 cut(s) 44, 45, 497, 557, 1124, 1260, 1539
BmrI ACTGGG 2 cut(s) 1701, 2208
BmsI GCATC 7 cut(s) 739, 819, 1285, 1363, 1467, 2393, 2888
BmuI ACTGGG 2 cut(s) 1701, 2208
BpiI GAAGAC 7 cut(s) 18, 99, 148, 1467, 1510, 1576, 2342
BpmI CTGGAG 1 cut(s) 479
Bpu1102I GCTNAGC 1 cut(s) 2390
Bpu14I TTCGAA 2 cut(s) 1635, 1696
BpuEI CTTGAG 6 cut(s) 2132, 2261, 2543, 2683, 2825, 2834
BpuMI CCSGG 4 cut(s) 44, 45, 557, 1260
Bsa29I ATCGAT 1 cut(s) 1643
BsaHI GRCGYC 4 cut(s) 27, 147, 319, 360
BsaWI WCCGGW 1 cut(s) 2258
BsaXI ACNNNNNCTCC 2 cut(s) 1803, 1833
Bsc4I CCNNNNNNNGG 2 cut(s) 270, 2095
Bse118I RCCGGY 3 cut(s) 473, 832, 2258
Bse1I ACTGG 3 cut(s) 962, 1707, 2214
Bse21I CCTNAGG 1 cut(s) 1113
Bse3DI GCAATG 1 cut(s) 1821
BseBI CCWGG 3 cut(s) 497, 1124, 1539
BseCI ATCGAT 1 cut(s) 1643
BseGI GGATG 6 cut(s) 913, 1279, 1482, 1597, 2938, 2973
BseLI CCNNNNNNNGG 2 cut(s) 270, 2095
BseMI GCAATG 1 cut(s) 1821
BseMII CTCAG 8 cut(s) 68, 1076, 1104, 1875, 2174, 2222, 2404, 2418
BseNI ACTGG 3 cut(s) 962, 1707, 2214
BseRI GAGGAG 5 cut(s) 102, 299, 457, 482, 1222
BseSI GKGCMC 1 cut(s) 380
BseX3I CGGCCG 1 cut(s) 231
BseXI GCAGC 1 cut(s) 321
BseYI CCCAGC 2 cut(s) 63, 2285
Bsh1236I CGCG 8 cut(s) 102, 144, 183, 253, 735, 2001, 2091, 2685
Bsh1285I CGRYCG 2 cut(s) 219, 234
BshFI GGCC 7 cut(s) 131, 233, 378, 455, 698, 1127, 2229
BshNI GGYRCC 2 cut(s) 2014, 2789
BshTI ACCGGT 1 cut(s) 2258
BshVI ATCGAT 1 cut(s) 1643
BsiEI CGRYCG 2 cut(s) 219, 234
BsiHKAI GWGCWC 1 cut(s) 651
BsiHKCI CYCGRG 2 cut(s) 43, 594
BsiSI CCGG 7 cut(s) 44, 55, 474, 557, 833, 1260, 2259
BslFI GGGAC 6 cut(s) 80, 200, 597, 1242, 1700, 2887
BslI CCNNNNNNNGG 2 cut(s) 270, 2095
BsmAI GTCTC 8 cut(s) 948, 1065, 1152, 1221, 1488, 2327, 2521, 2750
BsmBI CGTCTC 2 cut(s) 1065, 1221
BsmFI GGGAC 6 cut(s) 80, 200, 597, 1242, 1700, 2887
BsnI GGCC 7 cut(s) 131, 233, 378, 455, 698, 1127, 2229
BsoBI CYCGRG 2 cut(s) 43, 594
Bsp119I TTCGAA 2 cut(s) 1635, 1696
Bsp120I GGGCCC 1 cut(s) 376
Bsp1286I GDGCHC 2 cut(s) 380, 651
Bsp1407I TGTACA 1 cut(s) 1516
Bsp1720I GCTNAGC 1 cut(s) 2390
Bsp19I CCATGG 1 cut(s) 450
BspANI GGCC 7 cut(s) 131, 233, 378, 455, 698, 1127, 2229
BspCNI CTCAG 8 cut(s) 69, 1075, 1105, 1876, 2175, 2223, 2403, 2419
BspDI ATCGAT 1 cut(s) 1643
BspFNI CGCG 8 cut(s) 102, 144, 183, 253, 735, 2001, 2091, 2685
BspHI TCATGA 2 cut(s) 1446, 2140
BspLI GGNNCC 6 cut(s) 351, 377, 378, 1662, 2016, 2791
BspMAI CTGCAG 4 cut(s) 414, 769, 1239, 1723
BspPI GGATC 8 cut(s) 344, 357, 624, 1529, 1569, 1655, 1668, 2515
BspQI GCTCTTC 1 cut(s) 110
BspT104I TTCGAA 2 cut(s) 1635, 1696
BspT107I GGYRCC 2 cut(s) 2014, 2789
BsrDI GCAATG 1 cut(s) 1821
BsrFI RCCGGY 3 cut(s) 473, 832, 2258
BsrGI TGTACA 1 cut(s) 1516
BsrI ACTGG 3 cut(s) 962, 1707, 2214
BssAI RCCGGY 3 cut(s) 473, 832, 2258
BssNAI GTATAC 1 cut(s) 1613
BssNI GRCGYC 4 cut(s) 27, 147, 319, 360
BssSI CACGAG 1 cut(s) 773
BssT1I CCWWGG 4 cut(s) 450, 693, 1807, 2094
Bst1107I GTATAC 1 cut(s) 1613
Bst2BI CACGAG 1 cut(s) 773
Bst2UI CCWGG 3 cut(s) 497, 1124, 1539
Bst4CI ACNGT 6 cut(s) 173, 580, 1077, 1167, 2338, 2951
Bst6I CTCTTC 2 cut(s) 110, 1358
BstACI GRCGYC 4 cut(s) 27, 147, 319, 360
BstAUI TGTACA 1 cut(s) 1516
BstBI TTCGAA 2 cut(s) 1635, 1696
BstC8I GCNNGC 6 cut(s) 206, 291, 1469, 1544, 2003, 2637
BstDSI CCRYGG 3 cut(s) 142, 181, 450
BstEII GGTNACC 1 cut(s) 2084
BstF5I GGATG 6 cut(s) 913, 1279, 1482, 1597, 2938, 2973
BstFNI CGCG 8 cut(s) 102, 144, 183, 253, 735, 2001, 2091, 2685
BstH2I RGCGCY 2 cut(s) 450, 1070
BstHHI GCGC 7 cut(s) 104, 449, 1069, 2001, 2093, 2685, 2959
BstMAI GTCTC 8 cut(s) 948, 1065, 1152, 1221, 1488, 2327, 2521, 2750
BstMCI CGRYCG 2 cut(s) 219, 234
BstMWI GCNNNNNNNGC 3 cut(s) 309, 869, 1477
BstNI CCWGG 3 cut(s) 497, 1124, 1539
BstNSI RCATGY 3 cut(s) 1341, 1471, 1546
BstPI GGTNACC 1 cut(s) 2084
BstSCI CCNGG 7 cut(s) 42, 43, 495, 555, 1122, 1258, 1537
BstSFI CTRYAG 5 cut(s) 410, 765, 1235, 1719, 2884
BstSLI GKGCMC 1 cut(s) 380
BstUI CGCG 8 cut(s) 102, 144, 183, 253, 735, 2001, 2091, 2685
BstV1I GCAGC 1 cut(s) 321
BstV2I GAAGAC 7 cut(s) 18, 99, 148, 1467, 1510, 1576, 2342
BstX2I RGATCY 4 cut(s) 349, 1534, 1660, 2507
BstXI CCANNNNNNTGG 1 cut(s) 2293
BstYI RGATCY 4 cut(s) 349, 1534, 1660, 2507
BstZ17I GTATAC 1 cut(s) 1613
BstZI CGGCCG 1 cut(s) 231
Bsu15I ATCGAT 1 cut(s) 1643
Bsu36I CCTNAGG 1 cut(s) 1113
BsuRI GGCC 7 cut(s) 131, 233, 378, 455, 698, 1127, 2229
BsuTUI ATCGAT 1 cut(s) 1643
BtgI CCRYGG 3 cut(s) 142, 181, 450
BtgZI GCGATG 2 cut(s) 178, 716
BtrI CACGTC 1 cut(s) 70
BtsCI GGATG 6 cut(s) 913, 1279, 1482, 1597, 2938, 2973
BtsI GCAGTG 1 cut(s) 1091
BtsIMutI CAGTG 5 cut(s) 585, 1091, 2221, 2343, 2552
Cac8I GCNNGC 6 cut(s) 206, 291, 1469, 1544, 2003, 2637
CaiI CAGNNNCTG 2 cut(s) 957, 1243
CciI TCATGA 2 cut(s) 1446, 2140
CciNI GCGGCCGC 1 cut(s) 231
CfoI GCGC 7 cut(s) 104, 449, 1069, 2001, 2093, 2685, 2959
Cfr10I RCCGGY 3 cut(s) 473, 832, 2258
Cfr13I GGNCC 6 cut(s) 130, 178, 376, 377, 697, 1126
Cfr42I CCGCGG 2 cut(s) 145, 184
Cfr9I CCCGGG 1 cut(s) 43
ClaI ATCGAT 1 cut(s) 1643
CseI GACGC 4 cut(s) 35, 136, 308, 2628
Csp6I GTAC 3 cut(s) 124, 880, 1517
CspAI ACCGGT 1 cut(s) 2258
CviQI GTAC 3 cut(s) 124, 880, 1517
DraI TTTAAA 1 cut(s) 2727
DriI GACNNNNNGTC 1 cut(s) 1958
EaeI YGGCCR 2 cut(s) 231, 453
EagI CGGCCG 1 cut(s) 231
Eam1104I CTCTTC 2 cut(s) 110, 1358
Eam1105I GACNNNNNGTC 1 cut(s) 1958
EarI CTCTTC 2 cut(s) 110, 1358
EclXI CGGCCG 1 cut(s) 231
Eco130I CCWWGG 4 cut(s) 450, 693, 1807, 2094
Eco24I GRGCYC 1 cut(s) 380
Eco47I GGWCC 1 cut(s) 178
Eco52I CGGCCG 1 cut(s) 231
Eco57I CTGAAG 2 cut(s) 1253, 1409
Eco81I CCTNAGG 1 cut(s) 1113
Eco88I CYCGRG 2 cut(s) 43, 594
Eco91I GGTNACC 1 cut(s) 2084
EcoO109I RGGNCCY 3 cut(s) 130, 376, 697
EcoO65I GGTNACC 1 cut(s) 2084
EcoRI GAATTC 2 cut(s) 654, 2587
EcoRII CCWGG 3 cut(s) 495, 1122, 1537
EcoT14I CCWWGG 4 cut(s) 450, 693, 1807, 2094
EcoT38I GRGCYC 1 cut(s) 380
ErhI CCWWGG 4 cut(s) 450, 693, 1807, 2094
Esp3I CGTCTC 2 cut(s) 1065, 1221
FalI AAGNNNNNCTT 4 cut(s) 1307, 1339, 1740, 1772
FaqI GGGAC 6 cut(s) 80, 200, 597, 1242, 1700, 2887
FauI CCCGC 3 cut(s) 107, 176, 1994
FblI GTMKAC 2 cut(s) 1552, 1612
Fnu4HI GCNGC 8 cut(s) 142, 145, 231, 234, 310, 466, 736, 2686
FokI GGATG 5 cut(s) 920, 1286, 1489, 1604, 2945
FriOI GRGCYC 1 cut(s) 380
Fsp4HI GCNGC 8 cut(s) 142, 145, 231, 234, 310, 466, 736, 2686
FspBI CTAG 3 cut(s) 1301, 1328, 2735
GlaI GCGC 7 cut(s) 103, 448, 1068, 2000, 2092, 2684, 2958
GluI GCNGC 8 cut(s) 142, 145, 231, 234, 310, 466, 736, 2686
GsaI CCCAGC 2 cut(s) 67, 2289
GsuI CTGGAG 1 cut(s) 479
HaeII RGCGCY 2 cut(s) 450, 1070
HaeIII GGCC 7 cut(s) 131, 233, 378, 455, 698, 1127, 2229
HapII CCGG 7 cut(s) 44, 55, 474, 557, 833, 1260, 2259
HgaI GACGC 4 cut(s) 35, 136, 308, 2628
HhaI GCGC 7 cut(s) 104, 449, 1069, 2001, 2093, 2685, 2959
Hin1I GRCGYC 4 cut(s) 27, 147, 319, 360
Hin6I GCGC 7 cut(s) 102, 447, 1067, 1999, 2091, 2683, 2957
HinP1I GCGC 7 cut(s) 102, 447, 1067, 1999, 2091, 2683, 2957
HincII GTYRAC 1 cut(s) 39
HindII GTYRAC 1 cut(s) 39
HindIII AAGCTT 3 cut(s) 1749, 2369, 2810
HpaII CCGG 7 cut(s) 44, 55, 474, 557, 833, 1260, 2259
HphI GGTGA 4 cut(s) 1421, 1423, 1682, 2248
Hpy166II GTNNAC 6 cut(s) 39, 880, 1027, 1553, 1613, 2173
Hpy8I GTNNAC 6 cut(s) 39, 880, 1027, 1553, 1613, 2173
HpyAV CCTTC 5 cut(s) 962, 1384, 1444, 1853, 1941
HpyCH4III ACNGT 6 cut(s) 173, 580, 1077, 1167, 2338, 2951
HpyCH4IV ACGT 4 cut(s) 69, 360, 480, 1230
HpyF10VI GCNNNNNNNGC 3 cut(s) 309, 869, 1477
HpySE526I ACGT 4 cut(s) 69, 360, 480, 1230
Hsp92I GRCGYC 4 cut(s) 27, 147, 319, 360
HspAI GCGC 7 cut(s) 102, 447, 1067, 1999, 2091, 2683, 2957
KspI CCGCGG 2 cut(s) 145, 184
LguI GCTCTTC 1 cut(s) 110
LmnI GCTCC 4 cut(s) 286, 444, 1802, 1811
Lsp1109I GCAGC 1 cut(s) 321
LweI GCATC 7 cut(s) 739, 819, 1285, 1363, 1467, 2393, 2888
MaeI CTAG 3 cut(s) 1301, 1328, 2735
MaeII ACGT 4 cut(s) 69, 360, 480, 1230
MaeIII GTNAC 7 cut(s) 476, 985, 1082, 1723, 1896, 2006, 2084
MfeI CAATTG 3 cut(s) 1377, 1419, 2024
MflI RGATCY 4 cut(s) 349, 1534, 1660, 2507
MhlI GDGCHC 2 cut(s) 380, 651
MlsI TGGCCA 1 cut(s) 455
MluNI TGGCCA 1 cut(s) 455
MlyI GAGTC 5 cut(s) 162, 218, 679, 2138, 2356
MmeI TCCRAC 5 cut(s) 381, 388, 1032, 2698, 2917
Mox20I TGGCCA 1 cut(s) 455
MroXI GAANNNNTTC 2 cut(s) 1583, 2249
MscI TGGCCA 1 cut(s) 455
MseI TTAA 8 cut(s) 705, 846, 1280, 1842, 2124, 2469, 2726, 2762
Msp20I TGGCCA 1 cut(s) 455
MspA1I CMGCKG 3 cut(s) 144, 183, 1883
MspI CCGG 7 cut(s) 44, 55, 474, 557, 833, 1260, 2259
MspR9I CCNGG 7 cut(s) 44, 45, 497, 557, 1124, 1260, 1539
MunI CAATTG 3 cut(s) 1377, 1419, 2024
MvaI CCWGG 3 cut(s) 497, 1124, 1539
MvnI CGCG 8 cut(s) 102, 144, 183, 253, 735, 2001, 2091, 2685
MwoI GCNNNNNNNGC 3 cut(s) 309, 869, 1477
NciI CCSGG 4 cut(s) 44, 45, 557, 1260
NcoI CCATGG 1 cut(s) 450
NlaIV GGNNCC 6 cut(s) 351, 377, 378, 1662, 2016, 2791
NmeAIII GCCGAG 2 cut(s) 206, 1927
NotI GCGGCCGC 1 cut(s) 231
NspI RCATGY 3 cut(s) 1341, 1471, 1546
NspV TTCGAA 2 cut(s) 1635, 1696
PaeI GCATGC 2 cut(s) 1471, 1546
PaeR7I CTCGAG 1 cut(s) 594
PagI TCATGA 2 cut(s) 1446, 2140
PciSI GCTCTTC 1 cut(s) 110
PcsI WCGNNNNNNNCGW 2 cut(s) 21, 27
PdmI GAANNNNTTC 2 cut(s) 1583, 2249
PfoI TCCNGGA 1 cut(s) 495
PinAI ACCGGT 1 cut(s) 2258
PkrI GCNGC 8 cut(s) 143, 146, 232, 235, 311, 467, 737, 2687
Ple19I CGATCG 1 cut(s) 219
PleI GAGTC 5 cut(s) 161, 218, 679, 2138, 2355
PpsI GAGTC 5 cut(s) 161, 218, 679, 2138, 2355
PshBI ATTAAT 2 cut(s) 1842, 2762
Psp6I CCWGG 3 cut(s) 495, 1122, 1537
PspEI GGTNACC 1 cut(s) 2084
PspFI CCCAGC 2 cut(s) 63, 2285
PspGI CCWGG 3 cut(s) 495, 1122, 1537
PspN4I GGNNCC 6 cut(s) 351, 377, 378, 1662, 2016, 2791
PspOMI GGGCCC 1 cut(s) 376
PspPI GGNCC 6 cut(s) 130, 178, 376, 377, 697, 1126
PstI CTGCAG 4 cut(s) 414, 769, 1239, 1723
PstNI CAGNNNCTG 2 cut(s) 957, 1243
PsuI RGATCY 4 cut(s) 349, 1534, 1660, 2507
PvuI CGATCG 1 cut(s) 219
PvuII CAGCTG 1 cut(s) 1883
RsaI GTAC 3 cut(s) 125, 881, 1518
RsaNI GTAC 3 cut(s) 124, 880, 1517
SacII CCGCGG 2 cut(s) 145, 184
SapI GCTCTTC 1 cut(s) 110
SaqAI TTAA 8 cut(s) 705, 846, 1280, 1842, 2124, 2469, 2726, 2762
SatI GCNGC 8 cut(s) 142, 145, 231, 234, 310, 466, 736, 2686
Sau96I GGNCC 6 cut(s) 130, 178, 376, 377, 697, 1126
SchI GAGTC 5 cut(s) 162, 218, 679, 2138, 2356
ScrFI CCNGG 7 cut(s) 44, 45, 497, 557, 1124, 1260, 1539
SduI GDGCHC 2 cut(s) 380, 651
SfaNI GCATC 7 cut(s) 739, 819, 1285, 1363, 1467, 2393, 2888
SfcI CTRYAG 5 cut(s) 410, 765, 1235, 1719, 2884
Sfr274I CTCGAG 1 cut(s) 594
Sfr303I CCGCGG 2 cut(s) 145, 184
SfuI TTCGAA 2 cut(s) 1635, 1696
SgrBI CCGCGG 2 cut(s) 145, 184
SinI GGWCC 1 cut(s) 178
SlaI CTCGAG 1 cut(s) 594
SmaI CCCGGG 1 cut(s) 45
SmlI CTYRAG 7 cut(s) 594, 2111, 2240, 2522, 2662, 2804, 2813
SmoI CTYRAG 7 cut(s) 594, 2111, 2240, 2522, 2662, 2804, 2813
SphI GCATGC 2 cut(s) 1471, 1546
SspMI CTAG 3 cut(s) 1301, 1328, 2735
StyD4I CCNGG 7 cut(s) 42, 43, 495, 555, 1122, 1258, 1537
StyI CCWWGG 4 cut(s) 450, 693, 1807, 2094
TaaI ACNGT 6 cut(s) 173, 580, 1077, 1167, 2338, 2951
TaiI ACGT 4 cut(s) 72, 363, 483, 1233
TatI WGTACW 1 cut(s) 1516
TauI GCSGC 7 cut(s) 144, 147, 233, 236, 468, 738, 2688
Tru1I TTAA 8 cut(s) 705, 846, 1280, 1842, 2124, 2469, 2726, 2762
Tru9I TTAA 8 cut(s) 705, 846, 1280, 1842, 2124, 2469, 2726, 2762
TscAI CASTG 5 cut(s) 585, 1098, 2221, 2343, 2559
TseI GCWGC 1 cut(s) 309
TspMI CCCGGG 1 cut(s) 43
TspRI CASTG 5 cut(s) 585, 1098, 2221, 2343, 2559
VpaK11BI GGWCC 1 cut(s) 178
VspI ATTAAT 2 cut(s) 1842, 2762
XapI RAATTY 8 cut(s) 654, 1852, 1922, 2155, 2408, 2587, 2600, 2728
XbaI TCTAGA 1 cut(s) 1300
XceI RCATGY 3 cut(s) 1341, 1471, 1546
XhoI CTCGAG 1 cut(s) 594
XmaI CCCGGG 1 cut(s) 43
XmiI GTMKAC 2 cut(s) 1552, 1612
XmnI GAANNNNTTC 2 cut(s) 1583, 2249
XspI CTAG 3 cut(s) 1301, 1328, 2735
ZraI GACGTC 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.