Prupe.1G263300_v2.0.a1

5-formyltetrahydrofolate cyclo-ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0005536
Physical Location & Seq
Forward (+)
27176305 .. 27179192
2888 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G263300.2

Sequence Viewer

Length: 879 bp
ATGTTCAGTCACAGTAGAGCAAGCGCGAGCGAGCTCATAAACAGGGCAAAGGAGGCAGTGACGCTCATCACCCAGCCGCGCACGTTAGCTTCTCCTCCCCTTTTCACCTCGCGCCCCGCTAACGAACACCTTGCGTCCCAATCTCACCGCTCCTTCGCCACCATGAACCGGAACGACGACCCAATCGAAGCTCTATTCCAGCAGAAGCGCTCGCTCCGGTCCAGAATGCGCAAAGAGCTCAGAAACATGGACCCGGTTCGAAGATCCGAAGAAGATGCTGCAATTCAGAGCATTGTTGTAAATAATCCCTGGTTCAAATCTAGCAAGAGTGTATGTGCTTACATTAGCTCCCCTGCTTTACGAGAAGTCGATACGACAAGAATTGTTTCAGAAATTTTATCCAAACCAGCCAATGGGAGTGATGTGCCGAACAGGAAAAAGCTTTATGTTCCTCGTGTAGAGGATAGGAATAGCAATATGAGAATGCTCAGGATTTCCAGTGTTGATGATTTGATTGTGAACTCAATGAACATTTTGGAACCAGCTCTTTCAGATTCTGAGGGGAAGCAGCATGAAGATGTTATGGAGGCAAGGGATCCAGTTGACTTGTTCATCCTACCTGGGCTTGCATTTGACAGATGTGGAAGGCGTTTAGGCCGTAGTGGAGGCTACTATGATTTGTTCCTAAAGAAGTACCAAGAGCTTACAAAGGAACGAAAATGGAAGGAGCCCCTTCGTGTTGCACTTTCATATTCTATACAGATCGTGGAGGAAGGAGCCATAGCTGTCACTTCAAATGATGTTTCTGTGGATGCTCTTGTATCTCCAGCCGGTGTCATTCCGATAAGCCCTGCTGCTTGGGAGAGGAGCATGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00670 ko01100 map00670 map01100
Pfam Domains
Protein Families
5-FTHF_cyc-lig

Protein Analysis

293

Amino Acids

32.81

Weight (kDa)

9.2

Isoelectric Point (pI)

60.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 230
AccB7I CCANNNNNTGG 1 cut(s) 413
AccBSI CCGCTC 1 cut(s) 150
AccII CGCG 3 cut(s) 26, 79, 112
AciI CCGC 3 cut(s) 77, 117, 148
AclWI GGATC 3 cut(s) 258, 590, 603
AcsI RAATTY 1 cut(s) 393
AfaI GTAC 1 cut(s) 695
AfeI AGCGCT 1 cut(s) 209
AfiI CCNNNNNNNGG 2 cut(s) 168, 413
AgsI TTSAA 2 cut(s) 316, 795
AjnI CCWGG 2 cut(s) 308, 619
AluBI AGCT 9 cut(s) 34, 89, 191, 238, 348, 442, 545, 703, 785
AluI AGCT 9 cut(s) 34, 89, 191, 238, 348, 442, 545, 703, 785
Alw21I GWGCWC 2 cut(s) 36, 240
AlwI GGATC 3 cut(s) 258, 590, 603
AlwNI CAGNNNCTG 1 cut(s) 557
Aor51HI AGCGCT 1 cut(s) 209
AoxI GGCC 1 cut(s) 655
ApeKI GCWGC 3 cut(s) 278, 568, 854
ApoI RAATTY 1 cut(s) 393
Asp700I GAANNNNTTC 1 cut(s) 385
AspLEI GCGC 5 cut(s) 26, 81, 114, 210, 231
AspS9I GGNCC 2 cut(s) 219, 250
AsuC2I CCSGG 1 cut(s) 254
AsuHPI GGTGA 3 cut(s) 61, 97, 137
AsuII TTCGAA 1 cut(s) 259
AvaII GGWCC 2 cut(s) 219, 250
BamHI GGATCC 1 cut(s) 595
BanII GRGCYC 3 cut(s) 36, 240, 732
BauI CACGAG 1 cut(s) 453
Bbv12I GWGCWC 2 cut(s) 36, 240
BbvI GCAGC 3 cut(s) 265, 580, 841
BceAI ACGGC 1 cut(s) 642
BcgI CGANNNNNNTGC 4 cut(s) 113, 147, 257, 291
BciT130I CCWGG 2 cut(s) 310, 621
BcnI CCSGG 1 cut(s) 254
BfaI CTAG 1 cut(s) 321
BfoI RGCGCY 1 cut(s) 211
BisI GCNGC 4 cut(s) 77, 279, 569, 855
BlsI GCNGC 4 cut(s) 78, 280, 570, 856
Bme1390I CCNGG 3 cut(s) 254, 310, 621
Bme18I GGWCC 2 cut(s) 219, 250
BmgT120I GGNCC 2 cut(s) 219, 250
BmiI GGNNCC 5 cut(s) 252, 540, 597, 729, 778
BmrFI CCNGG 3 cut(s) 254, 310, 621
BmsI GCATC 2 cut(s) 265, 802
BpmI CTGGAG 1 cut(s) 810
Bpu10I CCTNAGC 1 cut(s) 488
Bpu14I TTCGAA 1 cut(s) 259
BpuMI CCSGG 1 cut(s) 254
BsaJI CCNNGG 2 cut(s) 308, 620
BsaWI WCCGGW 2 cut(s) 168, 216
BsaXI ACNNNNNCTCC 3 cut(s) 332, 362, 859
Bsc4I CCNNNNNNNGG 2 cut(s) 168, 413
Bse118I RCCGGY 1 cut(s) 830
Bse1I ACTGG 2 cut(s) 498, 599
BseBI CCWGG 2 cut(s) 310, 621
BseDI CCNNGG 2 cut(s) 308, 620
BseGI GGATG 2 cut(s) 612, 817
BseLI CCNNNNNNNGG 2 cut(s) 168, 413
BseMII CTCAG 3 cut(s) 253, 502, 549
BseNI ACTGG 2 cut(s) 498, 599
BseRI GAGGAG 1 cut(s) 84
BseXI GCAGC 3 cut(s) 265, 580, 841
BseYI CCCAGC 1 cut(s) 72
Bsh1236I CGCG 3 cut(s) 26, 79, 112
BshFI GGCC 1 cut(s) 657
BsiHKAI GWGCWC 2 cut(s) 36, 240
BsiSI CCGG 4 cut(s) 169, 217, 254, 831
BslFI GGGAC 1 cut(s) 121
BslI CCNNNNNNNGG 2 cut(s) 168, 413
BsmFI GGGAC 1 cut(s) 121
BsmI GAATGC 2 cut(s) 231, 489
BsnI GGCC 1 cut(s) 657
Bsp119I TTCGAA 1 cut(s) 259
Bsp1286I GDGCHC 3 cut(s) 36, 240, 732
Bsp143I GATC 3 cut(s) 263, 595, 762
BspACI CCGC 3 cut(s) 77, 117, 148
BspANI GGCC 1 cut(s) 657
BspCNI CTCAG 3 cut(s) 252, 501, 550
BspFNI CGCG 3 cut(s) 26, 79, 112
BspLI GGNNCC 5 cut(s) 252, 540, 597, 729, 778
BspPI GGATC 3 cut(s) 258, 590, 603
BspT104I TTCGAA 1 cut(s) 259
BsrBI CCGCTC 1 cut(s) 150
BsrFI RCCGGY 1 cut(s) 830
BsrI ACTGG 2 cut(s) 498, 599
BssAI RCCGGY 1 cut(s) 830
BssECI CCNNGG 2 cut(s) 308, 620
BssMI GATC 3 cut(s) 263, 595, 762
BssSI CACGAG 1 cut(s) 453
Bst2BI CACGAG 1 cut(s) 453
Bst2UI CCWGG 2 cut(s) 310, 621
Bst4CI ACNGT 1 cut(s) 14
BstBI TTCGAA 1 cut(s) 259
BstC8I GCNNGC 5 cut(s) 22, 28, 32, 212, 627
BstDEI CTNAG 3 cut(s) 239, 488, 558
BstF5I GGATG 2 cut(s) 612, 817
BstFNI CGCG 3 cut(s) 26, 79, 112
BstH2I RGCGCY 1 cut(s) 211
BstHHI GCGC 5 cut(s) 26, 81, 114, 210, 231
BstKTI GATC 3 cut(s) 266, 598, 765
BstMBI GATC 3 cut(s) 263, 595, 762
BstMWI GCNNNNNNNGC 2 cut(s) 53, 235
BstNI CCWGG 2 cut(s) 310, 621
BstSCI CCNGG 3 cut(s) 252, 308, 619
BstUI CGCG 3 cut(s) 26, 79, 112
BstV1I GCAGC 3 cut(s) 265, 580, 841
BstX2I RGATCY 2 cut(s) 263, 595
BstYI RGATCY 2 cut(s) 263, 595
BsuRI GGCC 1 cut(s) 657
BtsCI GGATG 2 cut(s) 612, 817
BtsI GCAGTG 1 cut(s) 63
BtsIMutI CAGTG 2 cut(s) 63, 505
Cac8I GCNNGC 5 cut(s) 22, 28, 32, 212, 627
CaiI CAGNNNCTG 1 cut(s) 557
CfoI GCGC 5 cut(s) 26, 81, 114, 210, 231
Cfr10I RCCGGY 1 cut(s) 830
Cfr13I GGNCC 2 cut(s) 219, 250
CseI GACGC 2 cut(s) 70, 123
Csp6I GTAC 1 cut(s) 694
CviAII CATG 4 cut(s) 163, 247, 572, 871
CviQI GTAC 1 cut(s) 694
DdeI CTNAG 3 cut(s) 239, 488, 558
DpnI GATC 3 cut(s) 265, 597, 764
DpnII GATC 3 cut(s) 263, 595, 762
Ecl136II GAGCTC 2 cut(s) 34, 238
Eco24I GRGCYC 3 cut(s) 36, 240, 732
Eco47I GGWCC 2 cut(s) 219, 250
Eco47III AGCGCT 1 cut(s) 209
Eco53kI GAGCTC 2 cut(s) 34, 238
EcoICRI GAGCTC 2 cut(s) 34, 238
EcoRII CCWGG 2 cut(s) 308, 619
EcoT38I GRGCYC 3 cut(s) 36, 240, 732
FaeI CATG 4 cut(s) 166, 250, 575, 874
FaqI GGGAC 1 cut(s) 121
FatI CATG 4 cut(s) 162, 246, 571, 870
FauI CCCGC 1 cut(s) 124
Fnu4HI GCNGC 4 cut(s) 77, 279, 569, 855
FokI GGATG 2 cut(s) 599, 824
FriOI GRGCYC 3 cut(s) 36, 240, 732
Fsp4HI GCNGC 4 cut(s) 77, 279, 569, 855
FspBI CTAG 1 cut(s) 321
FspI TGCGCA 1 cut(s) 230
GlaI GCGC 5 cut(s) 25, 80, 113, 209, 230
GluI GCNGC 4 cut(s) 77, 279, 569, 855
GsaI CCCAGC 1 cut(s) 76
GsuI CTGGAG 1 cut(s) 810
HaeII RGCGCY 1 cut(s) 211
HaeIII GGCC 1 cut(s) 657
HapII CCGG 4 cut(s) 169, 217, 254, 831
HgaI GACGC 2 cut(s) 70, 123
HhaI GCGC 5 cut(s) 26, 81, 114, 210, 231
Hin1II CATG 4 cut(s) 166, 250, 575, 874
Hin6I GCGC 5 cut(s) 24, 79, 112, 208, 229
HinP1I GCGC 5 cut(s) 24, 79, 112, 208, 229
HincII GTYRAC 1 cut(s) 604
HindII GTYRAC 1 cut(s) 604
HindIII AAGCTT 1 cut(s) 440
HinfI GANTC 1 cut(s) 554
HpaII CCGG 4 cut(s) 169, 217, 254, 831
HphI GGTGA 3 cut(s) 61, 97, 137
Hpy166II GTNNAC 2 cut(s) 520, 604
Hpy188I TCNGA 7 cut(s) 242, 268, 288, 391, 553, 559, 843
Hpy188III TCNNGA 2 cut(s) 222, 490
Hpy8I GTNNAC 2 cut(s) 520, 604
Hpy99I CGWCG 1 cut(s) 179
HpyAV CCTTC 5 cut(s) 163, 639, 718, 743, 767
HpyCH4III ACNGT 1 cut(s) 14
HpyCH4IV ACGT 1 cut(s) 83
HpyCH4V TGCA 3 cut(s) 281, 629, 743
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 235
HpyF3I CTNAG 3 cut(s) 239, 488, 558
HpySE526I ACGT 1 cut(s) 83
Hsp92II CATG 4 cut(s) 166, 250, 575, 874
HspAI GCGC 5 cut(s) 24, 79, 112, 208, 229
Kzo9I GATC 3 cut(s) 263, 595, 762
LmnI GCTCC 6 cut(s) 155, 219, 353, 727, 776, 867
Lsp1109I GCAGC 3 cut(s) 265, 580, 841
LweI GCATC 2 cut(s) 265, 802
MaeI CTAG 1 cut(s) 321
MaeII ACGT 1 cut(s) 83
MaeIII GTNAC 3 cut(s) 8, 58, 787
MalI GATC 3 cut(s) 265, 597, 764
MbiI CCGCTC 1 cut(s) 150
MboI GATC 3 cut(s) 263, 595, 762
MboII GAAGA 4 cut(s) 273, 281, 284, 587
MflI RGATCY 2 cut(s) 263, 595
MhlI GDGCHC 3 cut(s) 36, 240, 732
MluCI AATT 3 cut(s) 282, 381, 393
MroXI GAANNNNTTC 1 cut(s) 385
MseI TTAA 1 cut(s) 877
MslI CAYNNNNRTG 1 cut(s) 576
MspI CCGG 4 cut(s) 169, 217, 254, 831
MspR9I CCNGG 3 cut(s) 254, 310, 621
Mva1269I GAATGC 2 cut(s) 231, 489
MvaI CCWGG 2 cut(s) 310, 621
MvnI CGCG 3 cut(s) 26, 79, 112
MwoI GCNNNNNNNGC 2 cut(s) 53, 235
NciI CCSGG 1 cut(s) 254
NdeII GATC 3 cut(s) 263, 595, 762
NlaIII CATG 4 cut(s) 166, 250, 575, 874
NlaIV GGNNCC 5 cut(s) 252, 540, 597, 729, 778
NmuCI GTSAC 3 cut(s) 8, 58, 787
NsbI TGCGCA 1 cut(s) 230
NspV TTCGAA 1 cut(s) 259
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PctI GAATGC 2 cut(s) 231, 489
PdmI GAANNNNTTC 1 cut(s) 385
PfeI GAWTC 1 cut(s) 554
PflMI CCANNNNNTGG 1 cut(s) 413
PkrI GCNGC 4 cut(s) 78, 280, 570, 856
Psp124BI GAGCTC 2 cut(s) 36, 240
Psp6I CCWGG 2 cut(s) 308, 619
PspFI CCCAGC 1 cut(s) 72
PspGI CCWGG 2 cut(s) 308, 619
PspN4I GGNNCC 5 cut(s) 252, 540, 597, 729, 778
PspPI GGNCC 2 cut(s) 219, 250
PstNI CAGNNNCTG 1 cut(s) 557
PsuI RGATCY 2 cut(s) 263, 595
RsaI GTAC 1 cut(s) 695
RsaNI GTAC 1 cut(s) 694
RseI CAYNNNNRTG 1 cut(s) 576
SacI GAGCTC 2 cut(s) 36, 240
SaqAI TTAA 1 cut(s) 877
SatI GCNGC 4 cut(s) 77, 279, 569, 855
Sau3AI GATC 3 cut(s) 263, 595, 762
Sau96I GGNCC 2 cut(s) 219, 250
ScrFI CCNGG 3 cut(s) 254, 310, 621
SduI GDGCHC 3 cut(s) 36, 240, 732
SfaNI GCATC 2 cut(s) 265, 802
SfuI TTCGAA 1 cut(s) 259
SinI GGWCC 2 cut(s) 219, 250
SmiMI CAYNNNNRTG 1 cut(s) 576
Sse9I AATT 3 cut(s) 282, 381, 393
SsiI CCGC 3 cut(s) 77, 117, 148
SspMI CTAG 1 cut(s) 321
SstI GAGCTC 2 cut(s) 36, 240
StyD4I CCNGG 3 cut(s) 252, 308, 619
TaaI ACNGT 1 cut(s) 14
TaiI ACGT 1 cut(s) 86
TaqI TCGA 3 cut(s) 186, 259, 369
TasI AATT 3 cut(s) 282, 381, 393
TauI GCSGC 1 cut(s) 79
TfiI GAWTC 1 cut(s) 554
Tru1I TTAA 1 cut(s) 877
Tru9I TTAA 1 cut(s) 877
TscAI CASTG 2 cut(s) 63, 505
TseFI GTSAC 3 cut(s) 8, 58, 787
TseI GCWGC 3 cut(s) 278, 568, 854
Tsp45I GTSAC 3 cut(s) 8, 58, 787
TspDTI ATGAA 5 cut(s) 179, 542, 588, 601, 738
TspRI CASTG 2 cut(s) 63, 505
Van91I CCANNNNNTGG 1 cut(s) 413
VpaK11BI GGWCC 2 cut(s) 219, 250
XapI RAATTY 1 cut(s) 393
XmnI GAANNNNTTC 1 cut(s) 385
XspI CTAG 1 cut(s) 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.