pycom16g07170

5-formyltetrahydrofolate cyclo-ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0005536
Physical Location & Seq
Reverse (-)
4677391 .. 4679448
2058 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g07170.7

Sequence Viewer

Length: 1035 bp
ATGGTACTATTCAATTTACCTTTTATTTTATCCTTATCATTAAAATTCAAAGTTTTCAAACATATCCGACCCGAACTCTTCAACCGCGCCTACGCGCAACCAAAGAGCGCCAAATTAACGGAAAAACCCACAAAAGCACGAGAGCCACGAAGCATGTTCAGTCACAGCAGAGCAAGCGCGAGTGGGCTCATAAACGGGGCAAAGGAGGCGGTGGCGCTCATCACCCAGCCGCGCACGTTAGCTTCTCGTACCCTCTTCCCTTCTCGCTTAGCTAACGAACGCCTCGCATCCCATTCTCACCGCCCCTACGCCATCATGAACCGAGACGACAACGACCCAATCGAAGCTATATTCCGGCAGAAGCGGTTGCTCCGGTCCAGAATCCGCAAAGAGCTCAAGAATATGGACCCGGTTCGGAGGTCCGAAGAAGATGCTGCAATTCAGAGCATTGTTTTAAATGCTCCCTGGTTCCAATCCAGTAAGAGCATATGCGCTTACATAAGCTCTCCTGCTTTACGAGAAGTCGATACGTCAAGAATTGTTTCAGAAATTCTATCCAAACCTGCCAATGAGGGTGATGTGCAGAACAGGAAAAAGCTTTATGTTCCGCGTGTGGAGGATAGGAATAGCAACATGAGGATGCTTAGGATTTCCAGCGTTGATGATCTGATCGTGAACTCGATGGACATTTTGGAGCCAGCTCTTTTGGATTCTGATGGGAAACAACACGAAGATGTTTTGGAGGCAAGCGATCCAGTTGACTTGTTCATCCTACCCGGGCTTGCATTTGACAGATGTGGAAGCCGTTTAGGCCGTGGTGGAGGTTACTATGATTTGTTCCTAAAGAAGTACCAAGAGCTTACAAAGCAACGACAATGGAAGGAGCCCCTTCGAGTTGCGCTGTCATATTCTGTACAGATCGTGGAGGAAGGAGCCATAGCTGTCACTTCAAATGATGTTTCTGTGGATGCTCTTGCATCTCCAGCCGGTGTCATTCCCATAAGCCCCGCTGCTCAGGAGAGGAGCATGGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00670 ko01100 map00670 map01100
Pfam Domains
Protein Families
5-FTHF_cyc-lig

Protein Analysis

345

Amino Acids

38.62

Weight (kDa)

9.55

Isoelectric Point (pI)

52.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 571
AccII CGCG 5 cut(s) 87, 95, 179, 232, 610
AciI CCGC 8 cut(s) 85, 209, 230, 301, 364, 385, 608, 1008
AclWI GGATC 1 cut(s) 746
AcsI RAATTY 2 cut(s) 44, 549
AfaI GTAC 4 cut(s) 6, 250, 851, 915
AgsI TTSAA 5 cut(s) 13, 49, 58, 82, 951
AjnI CCWGG 1 cut(s) 464
AluBI AGCT 9 cut(s) 242, 272, 347, 394, 504, 598, 701, 859, 941
AluI AGCT 9 cut(s) 242, 272, 347, 394, 504, 598, 701, 859, 941
Alw21I GWGCWC 1 cut(s) 396
Alw26I GTCTC 1 cut(s) 318
AlwI GGATC 1 cut(s) 746
Ama87I CYCGRG 1 cut(s) 776
AoxI GGCC 1 cut(s) 811
ApeKI GCWGC 2 cut(s) 434, 1010
ApoI RAATTY 2 cut(s) 44, 549
Asp700I GAANNNNTTC 1 cut(s) 541
AspLEI GCGC 8 cut(s) 89, 97, 110, 179, 217, 234, 494, 901
AspS9I GGNCC 3 cut(s) 375, 406, 420
AsuC2I CCSGG 3 cut(s) 410, 777, 778
AsuHPI GGTGA 3 cut(s) 214, 290, 587
AvaI CYCGRG 1 cut(s) 776
AvaII GGWCC 3 cut(s) 375, 406, 420
BanII GRGCYC 3 cut(s) 189, 396, 888
BauI CACGAG 1 cut(s) 138
Bbv12I GWGCWC 1 cut(s) 396
BbvI GCAGC 2 cut(s) 421, 997
BccI CCATC 3 cut(s) 320, 676, 710
BceAI ACGGC 2 cut(s) 789, 798
BcgI CGANNNNNNTGC 2 cut(s) 413, 447
BciT130I CCWGG 1 cut(s) 466
BcnI CCSGG 3 cut(s) 410, 777, 778
BcoDI GTCTC 1 cut(s) 318
BfoI RGCGCY 2 cut(s) 111, 218
BfuAI ACCTGC 1 cut(s) 571
BglI GCCNNNNNGGC 1 cut(s) 810
BisI GCNGC 3 cut(s) 230, 435, 1011
BlpI GCTNAGC 1 cut(s) 268
BlsI GCNGC 3 cut(s) 231, 436, 1012
Bme1390I CCNGG 4 cut(s) 410, 466, 777, 778
Bme18I GGWCC 3 cut(s) 375, 406, 420
BmeT110I CYCGRG 1 cut(s) 776
BmgT120I GGNCC 3 cut(s) 375, 406, 420
BmiI GGNNCC 5 cut(s) 408, 470, 696, 885, 934
BmrFI CCNGG 4 cut(s) 410, 466, 777, 778
BmsI GCATC 5 cut(s) 296, 421, 630, 958, 986
BpmI CTGGAG 1 cut(s) 966
Bpu10I CCTNAGC 2 cut(s) 644, 1014
Bpu1102I GCTNAGC 1 cut(s) 268
BpuEI CTTGAG 1 cut(s) 380
BpuMI CCSGG 3 cut(s) 410, 777, 778
BsaJI CCNNGG 3 cut(s) 464, 776, 814
BsaWI WCCGGW 1 cut(s) 372
Bse118I RCCGGY 1 cut(s) 986
Bse1I ACTGG 2 cut(s) 477, 755
BseBI CCWGG 1 cut(s) 466
BseDI CCNNGG 3 cut(s) 464, 776, 814
BseGI GGATG 4 cut(s) 287, 645, 768, 973
BseMII CTCAG 1 cut(s) 1028
BseNI ACTGG 2 cut(s) 477, 755
BseXI GCAGC 2 cut(s) 421, 997
BseYI CCCAGC 1 cut(s) 225
BsgI GTGCAG 1 cut(s) 602
Bsh1236I CGCG 5 cut(s) 87, 95, 179, 232, 610
BshFI GGCC 1 cut(s) 813
BsiHKAI GWGCWC 1 cut(s) 396
BsiHKCI CYCGRG 1 cut(s) 776
BsiSI CCGG 5 cut(s) 355, 373, 410, 777, 987
BsmAI GTCTC 1 cut(s) 318
BsmBI CGTCTC 1 cut(s) 318
BsnI GGCC 1 cut(s) 813
BsoBI CYCGRG 1 cut(s) 776
Bsp1286I GDGCHC 3 cut(s) 189, 396, 888
Bsp1407I TGTACA 1 cut(s) 913
Bsp143I GATC 4 cut(s) 664, 669, 751, 918
Bsp1720I GCTNAGC 1 cut(s) 268
BspACI CCGC 8 cut(s) 85, 209, 230, 301, 364, 385, 608, 1008
BspANI GGCC 1 cut(s) 813
BspCNI CTCAG 1 cut(s) 1027
BspFNI CGCG 5 cut(s) 87, 95, 179, 232, 610
BspHI TCATGA 1 cut(s) 315
BspLI GGNNCC 5 cut(s) 408, 470, 696, 885, 934
BspMI ACCTGC 1 cut(s) 571
BspPI GGATC 1 cut(s) 746
BsrFI RCCGGY 1 cut(s) 986
BsrGI TGTACA 1 cut(s) 913
BsrI ACTGG 2 cut(s) 477, 755
BssAI RCCGGY 1 cut(s) 986
BssECI CCNNGG 3 cut(s) 464, 776, 814
BssMI GATC 4 cut(s) 664, 669, 751, 918
BssSI CACGAG 1 cut(s) 138
Bst2BI CACGAG 1 cut(s) 138
Bst2UI CCWGG 1 cut(s) 466
Bst6I CTCTTC 2 cut(s) 83, 260
BstAUI TGTACA 1 cut(s) 913
BstC8I GCNNGC 4 cut(s) 175, 699, 748, 783
BstDEI CTNAG 3 cut(s) 268, 644, 1014
BstDSI CCRYGG 1 cut(s) 814
BstF5I GGATG 4 cut(s) 287, 645, 768, 973
BstFNI CGCG 5 cut(s) 87, 95, 179, 232, 610
BstH2I RGCGCY 2 cut(s) 111, 218
BstHHI GCGC 8 cut(s) 89, 97, 110, 179, 217, 234, 494, 901
BstKTI GATC 4 cut(s) 667, 672, 754, 921
BstMAI GTCTC 1 cut(s) 318
BstMBI GATC 4 cut(s) 664, 669, 751, 918
BstMWI GCNNNNNNNGC 5 cut(s) 174, 206, 810, 865, 983
BstNI CCWGG 1 cut(s) 466
BstNSI RCATGY 1 cut(s) 157
BstSCI CCNGG 4 cut(s) 408, 464, 775, 776
BstUI CGCG 5 cut(s) 87, 95, 179, 232, 610
BstV1I GCAGC 2 cut(s) 421, 997
BsuRI GGCC 1 cut(s) 813
BtgI CCRYGG 1 cut(s) 814
BtsCI GGATG 4 cut(s) 287, 645, 768, 973
BveI ACCTGC 1 cut(s) 571
Cac8I GCNNGC 4 cut(s) 175, 699, 748, 783
CciI TCATGA 1 cut(s) 315
CfoI GCGC 8 cut(s) 89, 97, 110, 179, 217, 234, 494, 901
Cfr10I RCCGGY 1 cut(s) 986
Cfr13I GGNCC 3 cut(s) 375, 406, 420
Cfr9I CCCGGG 1 cut(s) 776
Csp6I GTAC 4 cut(s) 5, 249, 850, 914
CviAII CATG 4 cut(s) 154, 316, 634, 1027
CviQI GTAC 4 cut(s) 5, 249, 850, 914
DdeI CTNAG 3 cut(s) 268, 644, 1014
DpnI GATC 4 cut(s) 666, 671, 753, 920
DpnII GATC 4 cut(s) 664, 669, 751, 918
DraI TTTAAA 1 cut(s) 456
Eam1104I CTCTTC 2 cut(s) 83, 260
EarI CTCTTC 2 cut(s) 83, 260
Ecl136II GAGCTC 1 cut(s) 394
Eco24I GRGCYC 3 cut(s) 189, 396, 888
Eco47I GGWCC 3 cut(s) 375, 406, 420
Eco53kI GAGCTC 1 cut(s) 394
Eco88I CYCGRG 1 cut(s) 776
EcoICRI GAGCTC 1 cut(s) 394
EcoRII CCWGG 1 cut(s) 464
EcoT38I GRGCYC 3 cut(s) 189, 396, 888
Esp3I CGTCTC 1 cut(s) 318
FaeI CATG 4 cut(s) 157, 319, 637, 1030
FatI CATG 4 cut(s) 153, 315, 633, 1026
FauI CCCGC 1 cut(s) 1015
FauNDI CATATG 1 cut(s) 488
Fnu4HI GCNGC 3 cut(s) 230, 435, 1011
FokI GGATG 4 cut(s) 274, 652, 755, 980
FriOI GRGCYC 3 cut(s) 189, 396, 888
Fsp4HI GCNGC 3 cut(s) 230, 435, 1011
GlaI GCGC 8 cut(s) 88, 96, 109, 178, 216, 233, 493, 900
GluI GCNGC 3 cut(s) 230, 435, 1011
GsaI CCCAGC 1 cut(s) 229
GsuI CTGGAG 1 cut(s) 966
HaeII RGCGCY 2 cut(s) 111, 218
HaeIII GGCC 1 cut(s) 813
HapII CCGG 5 cut(s) 355, 373, 410, 777, 987
HhaI GCGC 8 cut(s) 89, 97, 110, 179, 217, 234, 494, 901
Hin1II CATG 4 cut(s) 157, 319, 637, 1030
Hin6I GCGC 8 cut(s) 87, 95, 108, 177, 215, 232, 492, 899
HinP1I GCGC 8 cut(s) 87, 95, 108, 177, 215, 232, 492, 899
HincII GTYRAC 1 cut(s) 760
HindII GTYRAC 1 cut(s) 760
HindIII AAGCTT 1 cut(s) 596
HinfI GANTC 2 cut(s) 381, 710
HpaII CCGG 5 cut(s) 355, 373, 410, 777, 987
HphI GGTGA 3 cut(s) 214, 290, 587
Hpy166II GTNNAC 2 cut(s) 676, 760
Hpy188I TCNGA 7 cut(s) 68, 417, 424, 444, 547, 669, 715
Hpy188III TCNNGA 6 cut(s) 316, 378, 397, 534, 673, 1016
Hpy8I GTNNAC 2 cut(s) 676, 760
HpyAV CCTTC 4 cut(s) 270, 874, 899, 923
HpyCH4IV ACGT 2 cut(s) 236, 530
HpyCH4V TGCA 4 cut(s) 437, 583, 785, 977
HpyF10VI GCNNNNNNNGC 5 cut(s) 174, 206, 810, 865, 983
HpyF3I CTNAG 3 cut(s) 268, 644, 1014
HpySE526I ACGT 2 cut(s) 236, 530
Hsp92II CATG 4 cut(s) 157, 319, 637, 1030
HspAI GCGC 8 cut(s) 87, 95, 108, 177, 215, 232, 492, 899
Kzo9I GATC 4 cut(s) 664, 669, 751, 918
LmnI GCTCC 6 cut(s) 375, 466, 694, 883, 932, 1023
Lsp1109I GCAGC 2 cut(s) 421, 997
LweI GCATC 5 cut(s) 296, 421, 630, 958, 986
MaeII ACGT 2 cut(s) 236, 530
MaeIII GTNAC 3 cut(s) 161, 824, 943
MalI GATC 4 cut(s) 666, 671, 753, 920
MboI GATC 4 cut(s) 664, 669, 751, 918
MboII GAAGA 5 cut(s) 70, 247, 437, 440, 743
MhlI GDGCHC 3 cut(s) 189, 396, 888
MluCI AATT 6 cut(s) 13, 44, 113, 438, 537, 549
MmeI TCCRAC 1 cut(s) 91
MroXI GAANNNNTTC 1 cut(s) 541
MseI TTAA 3 cut(s) 41, 116, 455
MslI CAYNNNNRTG 2 cut(s) 638, 732
MspA1I CMGCKG 1 cut(s) 1010
MspI CCGG 5 cut(s) 355, 373, 410, 777, 987
MspR9I CCNGG 4 cut(s) 410, 466, 777, 778
MvaI CCWGG 1 cut(s) 466
MvnI CGCG 5 cut(s) 87, 95, 179, 232, 610
MwoI GCNNNNNNNGC 5 cut(s) 174, 206, 810, 865, 983
NciI CCSGG 3 cut(s) 410, 777, 778
NdeI CATATG 1 cut(s) 488
NdeII GATC 4 cut(s) 664, 669, 751, 918
NlaIII CATG 4 cut(s) 157, 319, 637, 1030
NlaIV GGNNCC 5 cut(s) 408, 470, 696, 885, 934
NmuCI GTSAC 2 cut(s) 161, 943
NspI RCATGY 1 cut(s) 157
PagI TCATGA 1 cut(s) 315
PcsI WCGNNNNNNNCGW 2 cut(s) 145, 339
PdmI GAANNNNTTC 1 cut(s) 541
PfeI GAWTC 2 cut(s) 381, 710
PkrI GCNGC 3 cut(s) 231, 436, 1012
Psp124BI GAGCTC 1 cut(s) 396
Psp6I CCWGG 1 cut(s) 464
PspFI CCCAGC 1 cut(s) 225
PspGI CCWGG 1 cut(s) 464
PspN4I GGNNCC 5 cut(s) 408, 470, 696, 885, 934
PspPI GGNCC 3 cut(s) 375, 406, 420
RsaI GTAC 4 cut(s) 6, 250, 851, 915
RsaNI GTAC 4 cut(s) 5, 249, 850, 914
RseI CAYNNNNRTG 2 cut(s) 638, 732
SacI GAGCTC 1 cut(s) 396
SaqAI TTAA 3 cut(s) 41, 116, 455
SatI GCNGC 3 cut(s) 230, 435, 1011
Sau3AI GATC 4 cut(s) 664, 669, 751, 918
Sau96I GGNCC 3 cut(s) 375, 406, 420
ScrFI CCNGG 4 cut(s) 410, 466, 777, 778
SduI GDGCHC 3 cut(s) 189, 396, 888
SfaNI GCATC 5 cut(s) 296, 421, 630, 958, 986
SinI GGWCC 3 cut(s) 375, 406, 420
SmaI CCCGGG 1 cut(s) 778
SmiMI CAYNNNNRTG 2 cut(s) 638, 732
SmlI CTYRAG 1 cut(s) 395
SmoI CTYRAG 1 cut(s) 395
Sse9I AATT 6 cut(s) 13, 44, 113, 438, 537, 549
SsiI CCGC 8 cut(s) 85, 209, 230, 301, 364, 385, 608, 1008
SstI GAGCTC 1 cut(s) 396
StyD4I CCNGG 4 cut(s) 408, 464, 775, 776
TaiI ACGT 2 cut(s) 239, 533
TaqI TCGA 4 cut(s) 342, 525, 680, 892
TasI AATT 6 cut(s) 13, 44, 113, 438, 537, 549
TatI WGTACW 1 cut(s) 913
TauI GCSGC 1 cut(s) 232
TfiI GAWTC 2 cut(s) 381, 710
Tru1I TTAA 3 cut(s) 41, 116, 455
Tru9I TTAA 3 cut(s) 41, 116, 455
TseFI GTSAC 2 cut(s) 161, 943
TseI GCWGC 2 cut(s) 434, 1010
Tsp45I GTSAC 2 cut(s) 161, 943
TspDTI ATGAA 2 cut(s) 332, 757
TspGWI ACGGA 1 cut(s) 134
TspMI CCCGGG 1 cut(s) 776
VpaK11BI GGWCC 3 cut(s) 375, 406, 420
XapI RAATTY 2 cut(s) 44, 549
XceI RCATGY 1 cut(s) 157
XmaI CCCGGG 1 cut(s) 776
XmnI GAANNNNTTC 1 cut(s) 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.