Rh4DG426900

5-formyltetrahydrofolate cyclo-ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0005536
Physical Location & Seq
Forward (+)
63271196 .. 63273842
2647 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG426900.1

Sequence Viewer

Length: 618 bp
ATGTTGAGTCAGCACCACCGGCAAGCCAGCGCGCGTGAGCTCTTAAACACGGCAACGCAATTGGTGGCGGCGCTGATCACCCACCCGCACACGTTAGCATGCTCCGGTAACCAACGCCTTTCATCCCAATCTCACTGCTCCTTCGCCACCATGACCGGAAAGGACGAGTCGCTCGAAGCTATATTCCGGCGGAAGCACTCGCTCCGGTCCAGACTCCGCAAGGAGCTCAAGAACATGGACCCGACCCGGAGATCCGAAGAAGATGCTGCGATTCAGAGCATTGTGTTGACTGCTCCTTGGTTCAAGTACAGTAAGAGTATATGTGCTTATATAAGCTCTCCTGAGCTGCGAGAAGTAGATACTTCAAGGATTGTGTCAGAAATTCTATCGAATTCGGGCAATGGTCCAGAAGGTGATGTGCTGATCAGGAGAAAGCTTTACGTTCCGCGGGTTGAGGATAGGAATAGTAATATGCGCATGCTGAGGATTAACGGTGTTGATGATCTGATTGTGAAGTCTATGAACATTTTGGAACCTGCGCTTTCGGATGCCGAGGGGAAACAATATGAAGATGGTACCTTCTTTTTCAAACAACATAATCTTTGCATTCCTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00670 ko01100 map00670 map01100
Pfam Domains
Protein Families
5-FTHF_cyc-lig

Protein Analysis

205

Amino Acids

23.25

Weight (kDa)

8.94

Isoelectric Point (pI)

51.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5-FTHF_cyc-lig PF01812 65 - 184 7.3e-06 5-formyltetrahydrofolate cyclo-ligase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 476
Acc36I ACCTGC 1 cut(s) 544
Acc65I GGTACC 1 cut(s) 575
AccB1I GGYRCC 1 cut(s) 575
AccII CGCG 3 cut(s) 32, 34, 448
AciI CCGC 6 cut(s) 68, 86, 190, 217, 446, 448
AclWI GGATC 1 cut(s) 246
AcsI RAATTY 2 cut(s) 381, 391
AfaI GTAC 2 cut(s) 308, 577
AflIII ACRYGT 1 cut(s) 90
AgsI TTSAA 3 cut(s) 304, 366, 589
AluBI AGCT 6 cut(s) 40, 179, 226, 336, 346, 436
AluI AGCT 6 cut(s) 40, 179, 226, 336, 346, 436
Alw21I GWGCWC 2 cut(s) 42, 228
AlwI GGATC 1 cut(s) 246
ApeKI GCWGC 2 cut(s) 266, 346
ApoI RAATTY 2 cut(s) 381, 391
Asp718I GGTACC 1 cut(s) 575
AspLEI GCGC 5 cut(s) 32, 34, 73, 477, 541
AspS9I GGNCC 3 cut(s) 207, 238, 404
AsuC2I CCSGG 1 cut(s) 247
AsuHPI GGTGA 2 cut(s) 70, 425
AvaII GGWCC 3 cut(s) 207, 238, 404
BanI GGYRCC 1 cut(s) 575
BanII GRGCYC 2 cut(s) 42, 228
Bbv12I GWGCWC 2 cut(s) 42, 228
BbvCI CCTCAGC 1 cut(s) 482
BbvI GCAGC 2 cut(s) 253, 333
BccI CCATC 1 cut(s) 566
BceAI ACGGC 1 cut(s) 66
BcgI CGANNNNNNTGC 2 cut(s) 245, 279
BclI TGATCA 2 cut(s) 75, 423
BcnI CCSGG 1 cut(s) 247
BfoI RGCGCY 1 cut(s) 74
BfuAI ACCTGC 1 cut(s) 544
BisI GCNGC 3 cut(s) 69, 267, 347
BlsI GCNGC 3 cut(s) 70, 268, 348
Bme1390I CCNGG 1 cut(s) 247
Bme18I GGWCC 3 cut(s) 207, 238, 404
BmgT120I GGNCC 3 cut(s) 207, 238, 404
BmiI GGNNCC 3 cut(s) 240, 534, 577
BmrFI CCNGG 1 cut(s) 247
BmsI GCATC 2 cut(s) 253, 538
Bpu10I CCTNAGC 2 cut(s) 342, 482
BpuEI CTTGAG 1 cut(s) 212
BpuMI CCSGG 1 cut(s) 247
BsaJI CCNNGG 3 cut(s) 296, 446, 552
BsaWI WCCGGW 3 cut(s) 104, 155, 204
Bse118I RCCGGY 1 cut(s) 18
Bse3DI GCAATG 1 cut(s) 406
BseDI CCNNGG 3 cut(s) 296, 446, 552
BseGI GGATG 2 cut(s) 122, 553
BseMI GCAATG 1 cut(s) 406
BseMII CTCAG 2 cut(s) 333, 473
BsePI GCGCGC 1 cut(s) 30
BseXI GCAGC 2 cut(s) 253, 333
Bsh1236I CGCG 3 cut(s) 32, 34, 448
BshNI GGYRCC 1 cut(s) 575
BsiHKAI GWGCWC 2 cut(s) 42, 228
BsiSI CCGG 6 cut(s) 19, 105, 156, 187, 205, 247
BsmI GAATGC 1 cut(s) 606
Bsp1286I GDGCHC 2 cut(s) 42, 228
Bsp143I GATC 4 cut(s) 75, 251, 423, 502
BspACI CCGC 6 cut(s) 68, 86, 190, 217, 446, 448
BspCNI CTCAG 2 cut(s) 334, 474
BspFNI CGCG 3 cut(s) 32, 34, 448
BspLI GGNNCC 3 cut(s) 240, 534, 577
BspMI ACCTGC 1 cut(s) 544
BspPI GGATC 1 cut(s) 246
BspT107I GGYRCC 1 cut(s) 575
BsrDI GCAATG 1 cut(s) 406
BsrFI RCCGGY 1 cut(s) 18
BssAI RCCGGY 1 cut(s) 18
BssECI CCNNGG 3 cut(s) 296, 446, 552
BssHII GCGCGC 1 cut(s) 30
BssMI GATC 4 cut(s) 75, 251, 423, 502
BssT1I CCWWGG 1 cut(s) 296
Bst4CI ACNGT 2 cut(s) 311, 494
BstC8I GCNNGC 5 cut(s) 24, 28, 32, 100, 479
BstDEI CTNAG 2 cut(s) 342, 482
BstDSI CCRYGG 1 cut(s) 446
BstEII GGTNACC 1 cut(s) 107
BstF5I GGATG 2 cut(s) 122, 553
BstFNI CGCG 3 cut(s) 32, 34, 448
BstH2I RGCGCY 1 cut(s) 74
BstHHI GCGC 5 cut(s) 32, 34, 73, 477, 541
BstKTI GATC 4 cut(s) 78, 254, 426, 505
BstMBI GATC 4 cut(s) 75, 251, 423, 502
BstMWI GCNNNNNNNGC 1 cut(s) 19
BstNSI RCATGY 2 cut(s) 102, 481
BstPI GGTNACC 1 cut(s) 107
BstSCI CCNGG 1 cut(s) 245
BstUI CGCG 3 cut(s) 32, 34, 448
BstV1I GCAGC 2 cut(s) 253, 333
BstX2I RGATCY 1 cut(s) 251
BstYI RGATCY 1 cut(s) 251
BtgI CCRYGG 1 cut(s) 446
BtsCI GGATG 2 cut(s) 122, 553
BtsI GCAGTG 1 cut(s) 133
BtsIMutI CAGTG 1 cut(s) 133
BveI ACCTGC 1 cut(s) 544
Cac8I GCNNGC 5 cut(s) 24, 28, 32, 100, 479
CfoI GCGC 5 cut(s) 32, 34, 73, 477, 541
Cfr10I RCCGGY 1 cut(s) 18
Cfr13I GGNCC 3 cut(s) 207, 238, 404
Cfr42I CCGCGG 1 cut(s) 449
Csp6I GTAC 2 cut(s) 307, 576
CviAII CATG 4 cut(s) 99, 151, 235, 478
CviJI RGCY 7 cut(s) 26, 40, 179, 226, 336, 346, 436
CviKI_1 RGCY 7 cut(s) 26, 40, 179, 226, 336, 346, 436
CviQI GTAC 2 cut(s) 307, 576
DdeI CTNAG 2 cut(s) 342, 482
DpnI GATC 4 cut(s) 77, 253, 425, 504
DpnII GATC 4 cut(s) 75, 251, 423, 502
EciI GGCGGA 1 cut(s) 205
Ecl136II GAGCTC 2 cut(s) 40, 226
Eco130I CCWWGG 1 cut(s) 296
Eco24I GRGCYC 2 cut(s) 42, 228
Eco47I GGWCC 3 cut(s) 207, 238, 404
Eco53kI GAGCTC 2 cut(s) 40, 226
Eco91I GGTNACC 1 cut(s) 107
EcoICRI GAGCTC 2 cut(s) 40, 226
EcoO65I GGTNACC 1 cut(s) 107
EcoRI GAATTC 1 cut(s) 391
EcoT14I CCWWGG 1 cut(s) 296
EcoT38I GRGCYC 2 cut(s) 42, 228
ErhI CCWWGG 1 cut(s) 296
FaeI CATG 4 cut(s) 102, 154, 238, 481
FatI CATG 4 cut(s) 98, 150, 234, 477
FauI CCCGC 2 cut(s) 93, 441
FbaI TGATCA 2 cut(s) 75, 423
Fnu4HI GCNGC 3 cut(s) 69, 267, 347
FokI GGATG 2 cut(s) 109, 560
FriOI GRGCYC 2 cut(s) 42, 228
Fsp4HI GCNGC 3 cut(s) 69, 267, 347
FspAI RTGCGCAY 1 cut(s) 476
FspI TGCGCA 1 cut(s) 476
GlaI GCGC 5 cut(s) 31, 33, 72, 476, 540
GluI GCNGC 3 cut(s) 69, 267, 347
HaeII RGCGCY 1 cut(s) 74
HapII CCGG 6 cut(s) 19, 105, 156, 187, 205, 247
HhaI GCGC 5 cut(s) 32, 34, 73, 477, 541
Hin1II CATG 4 cut(s) 102, 154, 238, 481
Hin6I GCGC 5 cut(s) 30, 32, 71, 475, 539
HinP1I GCGC 5 cut(s) 30, 32, 71, 475, 539
HincII GTYRAC 1 cut(s) 288
HindII GTYRAC 1 cut(s) 288
HindIII AAGCTT 1 cut(s) 434
HinfI GANTC 4 cut(s) 7, 167, 213, 271
HpaII CCGG 6 cut(s) 19, 105, 156, 187, 205, 247
HphI GGTGA 2 cut(s) 70, 425
Hpy166II GTNNAC 1 cut(s) 288
Hpy188I TCNGA 6 cut(s) 256, 276, 379, 507, 547, 617
Hpy188III TCNNGA 5 cut(s) 210, 229, 341, 407, 427
Hpy8I GTNNAC 1 cut(s) 288
HpyAV CCTTC 3 cut(s) 151, 404, 589
HpyCH4III ACNGT 2 cut(s) 311, 494
HpyCH4IV ACGT 2 cut(s) 92, 441
HpyCH4V TGCA 1 cut(s) 606
HpyF10VI GCNNNNNNNGC 1 cut(s) 19
HpyF3I CTNAG 2 cut(s) 342, 482
HpySE526I ACGT 2 cut(s) 92, 441
Hsp92II CATG 4 cut(s) 102, 154, 238, 481
HspAI GCGC 5 cut(s) 30, 32, 71, 475, 539
KpnI GGTACC 1 cut(s) 579
Ksp22I TGATCA 2 cut(s) 75, 423
KspI CCGCGG 1 cut(s) 449
Kzo9I GATC 4 cut(s) 75, 251, 423, 502
LmnI GCTCC 5 cut(s) 107, 143, 207, 223, 298
Lsp1109I GCAGC 2 cut(s) 253, 333
LweI GCATC 2 cut(s) 253, 538
MaeII ACGT 2 cut(s) 92, 441
MaeIII GTNAC 1 cut(s) 107
MalI GATC 4 cut(s) 77, 253, 425, 504
MboI GATC 4 cut(s) 75, 251, 423, 502
MboII GAAGA 3 cut(s) 269, 272, 581
MfeI CAATTG 1 cut(s) 59
MflI RGATCY 1 cut(s) 251
MhlI GDGCHC 2 cut(s) 42, 228
MluCI AATT 3 cut(s) 59, 381, 391
MlyI GAGTC 3 cut(s) 16, 176, 207
MnlI CCTC 3 cut(s) 448, 477, 547
MseI TTAA 2 cut(s) 44, 489
MspA1I CMGCKG 1 cut(s) 448
MspI CCGG 6 cut(s) 19, 105, 156, 187, 205, 247
MspR9I CCNGG 1 cut(s) 247
MunI CAATTG 1 cut(s) 59
Mva1269I GAATGC 1 cut(s) 606
MvnI CGCG 3 cut(s) 32, 34, 448
MwoI GCNNNNNNNGC 1 cut(s) 19
NciI CCSGG 1 cut(s) 247
NdeII GATC 4 cut(s) 75, 251, 423, 502
NlaIII CATG 4 cut(s) 102, 154, 238, 481
NlaIV GGNNCC 3 cut(s) 240, 534, 577
NmeAIII GCCGAG 1 cut(s) 577
NsbI TGCGCA 1 cut(s) 476
NspI RCATGY 2 cut(s) 102, 481
PaeI GCATGC 2 cut(s) 102, 481
PauI GCGCGC 1 cut(s) 30
PcsI WCGNNNNNNNCGW 1 cut(s) 171
PctI GAATGC 1 cut(s) 606
PfeI GAWTC 1 cut(s) 271
PkrI GCNGC 3 cut(s) 70, 268, 348
PleI GAGTC 3 cut(s) 15, 175, 207
PpsI GAGTC 3 cut(s) 15, 175, 207
Psp124BI GAGCTC 2 cut(s) 42, 228
PspEI GGTNACC 1 cut(s) 107
PspN4I GGNNCC 3 cut(s) 240, 534, 577
PspPI GGNCC 3 cut(s) 207, 238, 404
PsuI RGATCY 1 cut(s) 251
PteI GCGCGC 1 cut(s) 30
RsaI GTAC 2 cut(s) 308, 577
RsaNI GTAC 2 cut(s) 307, 576
SacI GAGCTC 2 cut(s) 42, 228
SacII CCGCGG 1 cut(s) 449
SaqAI TTAA 2 cut(s) 44, 489
SatI GCNGC 3 cut(s) 69, 267, 347
Sau3AI GATC 4 cut(s) 75, 251, 423, 502
Sau96I GGNCC 3 cut(s) 207, 238, 404
SchI GAGTC 3 cut(s) 16, 176, 207
ScrFI CCNGG 1 cut(s) 247
SduI GDGCHC 2 cut(s) 42, 228
SfaNI GCATC 2 cut(s) 253, 538
Sfr303I CCGCGG 1 cut(s) 449
SgrBI CCGCGG 1 cut(s) 449
SinI GGWCC 3 cut(s) 207, 238, 404
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
SphI GCATGC 2 cut(s) 102, 481
Sse9I AATT 3 cut(s) 59, 381, 391
SsiI CCGC 6 cut(s) 68, 86, 190, 217, 446, 448
SstI GAGCTC 2 cut(s) 42, 228
StyD4I CCNGG 1 cut(s) 245
StyI CCWWGG 1 cut(s) 296
TaaI ACNGT 2 cut(s) 311, 494
TaiI ACGT 2 cut(s) 95, 444
TaqI TCGA 2 cut(s) 174, 389
TasI AATT 3 cut(s) 59, 381, 391
TatI WGTACW 1 cut(s) 306
TauI GCSGC 1 cut(s) 71
TfiI GAWTC 1 cut(s) 271
Tru1I TTAA 2 cut(s) 44, 489
Tru9I TTAA 2 cut(s) 44, 489
TscAI CASTG 1 cut(s) 140
TseI GCWGC 2 cut(s) 266, 346
TspDTI ATGAA 3 cut(s) 111, 536, 582
TspRI CASTG 1 cut(s) 140
VpaK11BI GGWCC 3 cut(s) 207, 238, 404
XapI RAATTY 2 cut(s) 381, 391
XceI RCATGY 2 cut(s) 102, 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.