RchiOBHm_Chr4g0445021

5-formyltetrahydrofolate cyclo-ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0005536
Physical Location & Seq
Forward (+)
65625265 .. 65628000
2736 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41264

Sequence Viewer

Length: 870 bp
ATGTTGAGTCAGCACCACCGGCAAGCCAGCGCGCGTGAGCTCTTAAACACGGCAACGCAATTGGTGGCGGCGCTGATCACCCACCCGCACACGTTAGCATGCTCCGGTAACCAACGCCTTTCATCCCAATCTCACTGCTCCTTCGCCACCATGACCGGAAAGGACGAGTCGCTCGAAGCTATATTCCGGCGGAAGCACTCGCTCCGGTCCAGACTCCGCAAGGAGCTCAAGAACATGGACCCGACCCGGAGATCCGAAGAAGATGCTGCGATTCAGAGCATTGTGTTGACTGCTCCTTGGTTCAAGTACAGTAAGAGTATATGTGCTTATATAAGCTCTCCTGAGCTGCGAGAAGTAGATACTTCAAGGATTGTGTCAGAAATTCTATCGAATTCGGGCAATGGTCCAGAAGGTGATGTGCTGATCAGGAGAAAGCTTTACGTTCCGCGGGTTGAGGATAGGAATAGTAATATGCGCATGCTGAGGATTAACGGTGTTGATGATCTGATTGTGAAGTCTATGAACATTTTGGAACCTGCGCTTTCGGATGCCGAGGGGAAACAATATGAAGATGCTAGGGAGGCAAAGGATCCAGTTGACTTGTTCATCCTACCTGGTCTTGCTTTTGACAGATGTGGAAGACGTTTAGGCCGTAGTGGAGGTTATTATGACATGTTCCTAAAGAAGTACCAAAAGCTCGCAAAAGATCGGAAATGGAAGGATCCCGTCTGTGTTGCGCTATCATATTCAATACAGATCATGGAGGAAGGAGCCATACCTGTCACTTCGAATGATGTTTCTGTGGATGCTCTTGTATCCCCAGCTGGTTTTGTTCCAATAAGCCCAGCTGCTTGGAAGAGATGTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00670 ko01100 map00670 map01100
Pfam Domains
Protein Families
5-FTHF_cyc-lig

Protein Analysis

289

Amino Acids

32.38

Weight (kDa)

9.08

Isoelectric Point (pI)

50.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5-FTHF_cyc-lig PF01812 65 - 274 1.4e-27 5-formyltetrahydrofolate cyclo-ligase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 476
Acc36I ACCTGC 1 cut(s) 544
AccII CGCG 3 cut(s) 32, 34, 448
AciI CCGC 6 cut(s) 68, 86, 190, 217, 446, 448
AclWI GGATC 5 cut(s) 246, 584, 597, 716, 729
AcsI RAATTY 2 cut(s) 381, 391
AfaI GTAC 2 cut(s) 308, 689
AflIII ACRYGT 2 cut(s) 90, 672
AgsI TTSAA 3 cut(s) 304, 366, 750
AjnI CCWGG 1 cut(s) 613
AluBI AGCT 9 cut(s) 40, 179, 226, 336, 346, 436, 697, 824, 848
AluI AGCT 9 cut(s) 40, 179, 226, 336, 346, 436, 697, 824, 848
Alw21I GWGCWC 2 cut(s) 42, 228
AlwI GGATC 5 cut(s) 246, 584, 597, 716, 729
AoxI GGCC 1 cut(s) 649
ApeKI GCWGC 3 cut(s) 266, 346, 848
ApoI RAATTY 2 cut(s) 381, 391
AspLEI GCGC 6 cut(s) 32, 34, 73, 477, 541, 739
AspS9I GGNCC 3 cut(s) 207, 238, 404
AsuC2I CCSGG 1 cut(s) 247
AsuHPI GGTGA 2 cut(s) 70, 425
AsuII TTCGAA 1 cut(s) 788
AvaII GGWCC 3 cut(s) 207, 238, 404
BamHI GGATCC 2 cut(s) 589, 721
BanII GRGCYC 2 cut(s) 42, 228
BarI GAAGNNNNNNTAC 2 cut(s) 759, 791
BbsI GAAGAC 1 cut(s) 646
Bbv12I GWGCWC 2 cut(s) 42, 228
BbvCI CCTCAGC 1 cut(s) 482
BbvI GCAGC 3 cut(s) 253, 333, 835
BceAI ACGGC 2 cut(s) 66, 636
BcgI CGANNNNNNTGC 2 cut(s) 245, 279
BciT130I CCWGG 1 cut(s) 615
BciVI GTATCC 1 cut(s) 826
BclI TGATCA 2 cut(s) 75, 423
BcnI CCSGG 1 cut(s) 247
BfaI CTAG 1 cut(s) 576
BfoI RGCGCY 1 cut(s) 74
BfuAI ACCTGC 1 cut(s) 544
BfuI GTATCC 1 cut(s) 826
BisI GCNGC 4 cut(s) 69, 267, 347, 849
BlsI GCNGC 4 cut(s) 70, 268, 348, 850
Bme1390I CCNGG 2 cut(s) 247, 615
Bme18I GGWCC 3 cut(s) 207, 238, 404
BmgT120I GGNCC 3 cut(s) 207, 238, 404
BmiI GGNNCC 5 cut(s) 240, 534, 591, 723, 772
BmrFI CCNGG 2 cut(s) 247, 615
BmsI GCATC 4 cut(s) 253, 538, 562, 796
BpiI GAAGAC 1 cut(s) 646
Bpu10I CCTNAGC 2 cut(s) 342, 482
Bpu14I TTCGAA 1 cut(s) 788
BpuEI CTTGAG 1 cut(s) 212
BpuMI CCSGG 1 cut(s) 247
BsaJI CCNNGG 3 cut(s) 296, 446, 552
BsaWI WCCGGW 3 cut(s) 104, 155, 204
Bse118I RCCGGY 1 cut(s) 18
Bse1I ACTGG 1 cut(s) 593
Bse3DI GCAATG 1 cut(s) 406
BseBI CCWGG 1 cut(s) 615
BseDI CCNNGG 3 cut(s) 296, 446, 552
BseGI GGATG 4 cut(s) 122, 553, 606, 811
BseMI GCAATG 1 cut(s) 406
BseMII CTCAG 2 cut(s) 333, 473
BseNI ACTGG 1 cut(s) 593
BsePI GCGCGC 1 cut(s) 30
BseXI GCAGC 3 cut(s) 253, 333, 835
BseYI CCCAGC 2 cut(s) 820, 844
Bsh1236I CGCG 3 cut(s) 32, 34, 448
BshFI GGCC 1 cut(s) 651
BsiHKAI GWGCWC 2 cut(s) 42, 228
BsiSI CCGG 6 cut(s) 19, 105, 156, 187, 205, 247
BsnI GGCC 1 cut(s) 651
Bsp119I TTCGAA 1 cut(s) 788
Bsp1286I GDGCHC 2 cut(s) 42, 228
Bsp143I GATC 8 cut(s) 75, 251, 423, 502, 589, 706, 721, 756
BspACI CCGC 6 cut(s) 68, 86, 190, 217, 446, 448
BspANI GGCC 1 cut(s) 651
BspCNI CTCAG 2 cut(s) 334, 474
BspFNI CGCG 3 cut(s) 32, 34, 448
BspLI GGNNCC 5 cut(s) 240, 534, 591, 723, 772
BspMI ACCTGC 1 cut(s) 544
BspPI GGATC 5 cut(s) 246, 584, 597, 716, 729
BspT104I TTCGAA 1 cut(s) 788
BsrDI GCAATG 1 cut(s) 406
BsrFI RCCGGY 1 cut(s) 18
BsrI ACTGG 1 cut(s) 593
BssAI RCCGGY 1 cut(s) 18
BssECI CCNNGG 3 cut(s) 296, 446, 552
BssHII GCGCGC 1 cut(s) 30
BssMI GATC 8 cut(s) 75, 251, 423, 502, 589, 706, 721, 756
BssT1I CCWWGG 1 cut(s) 296
Bst2UI CCWGG 1 cut(s) 615
Bst4CI ACNGT 2 cut(s) 311, 494
Bst6I CTCTTC 1 cut(s) 851
BstBI TTCGAA 1 cut(s) 788
BstC8I GCNNGC 6 cut(s) 24, 28, 32, 100, 479, 699
BstDEI CTNAG 2 cut(s) 342, 482
BstDSI CCRYGG 1 cut(s) 446
BstEII GGTNACC 1 cut(s) 107
BstF5I GGATG 4 cut(s) 122, 553, 606, 811
BstFNI CGCG 3 cut(s) 32, 34, 448
BstH2I RGCGCY 1 cut(s) 74
BstHHI GCGC 6 cut(s) 32, 34, 73, 477, 541, 739
BstKTI GATC 8 cut(s) 78, 254, 426, 505, 592, 709, 724, 759
BstMBI GATC 8 cut(s) 75, 251, 423, 502, 589, 706, 721, 756
BstMWI GCNNNNNNNGC 2 cut(s) 19, 581
BstNI CCWGG 1 cut(s) 615
BstNSI RCATGY 3 cut(s) 102, 481, 676
BstPI GGTNACC 1 cut(s) 107
BstSCI CCNGG 2 cut(s) 245, 613
BstUI CGCG 3 cut(s) 32, 34, 448
BstV1I GCAGC 3 cut(s) 253, 333, 835
BstV2I GAAGAC 1 cut(s) 646
BstX2I RGATCY 3 cut(s) 251, 589, 721
BstXI CCANNNNNNTGG 1 cut(s) 852
BstYI RGATCY 3 cut(s) 251, 589, 721
BsuI GTATCC 1 cut(s) 826
BsuRI GGCC 1 cut(s) 651
BtgI CCRYGG 1 cut(s) 446
BtsCI GGATG 4 cut(s) 122, 553, 606, 811
BtsI GCAGTG 1 cut(s) 133
BtsIMutI CAGTG 1 cut(s) 133
BveI ACCTGC 1 cut(s) 544
Cac8I GCNNGC 6 cut(s) 24, 28, 32, 100, 479, 699
CfoI GCGC 6 cut(s) 32, 34, 73, 477, 541, 739
Cfr10I RCCGGY 1 cut(s) 18
Cfr13I GGNCC 3 cut(s) 207, 238, 404
Cfr42I CCGCGG 1 cut(s) 449
CsiI ACCWGGT 1 cut(s) 613
Csp6I GTAC 2 cut(s) 307, 688
CviAII CATG 6 cut(s) 99, 151, 235, 478, 673, 760
CviQI GTAC 2 cut(s) 307, 688
DdeI CTNAG 2 cut(s) 342, 482
DpnI GATC 8 cut(s) 77, 253, 425, 504, 591, 708, 723, 758
DpnII GATC 8 cut(s) 75, 251, 423, 502, 589, 706, 721, 756
Eam1104I CTCTTC 1 cut(s) 851
EarI CTCTTC 1 cut(s) 851
EciI GGCGGA 1 cut(s) 205
Ecl136II GAGCTC 2 cut(s) 40, 226
Eco130I CCWWGG 1 cut(s) 296
Eco24I GRGCYC 2 cut(s) 42, 228
Eco47I GGWCC 3 cut(s) 207, 238, 404
Eco53kI GAGCTC 2 cut(s) 40, 226
Eco91I GGTNACC 1 cut(s) 107
EcoICRI GAGCTC 2 cut(s) 40, 226
EcoO65I GGTNACC 1 cut(s) 107
EcoRI GAATTC 1 cut(s) 391
EcoRII CCWGG 1 cut(s) 613
EcoT14I CCWWGG 1 cut(s) 296
EcoT38I GRGCYC 2 cut(s) 42, 228
ErhI CCWWGG 1 cut(s) 296
FaeI CATG 6 cut(s) 102, 154, 238, 481, 676, 763
FatI CATG 6 cut(s) 98, 150, 234, 477, 672, 759
FauI CCCGC 2 cut(s) 93, 441
FbaI TGATCA 2 cut(s) 75, 423
Fnu4HI GCNGC 4 cut(s) 69, 267, 347, 849
FokI GGATG 4 cut(s) 109, 560, 593, 818
FriOI GRGCYC 2 cut(s) 42, 228
Fsp4HI GCNGC 4 cut(s) 69, 267, 347, 849
FspAI RTGCGCAY 1 cut(s) 476
FspBI CTAG 1 cut(s) 576
FspI TGCGCA 1 cut(s) 476
GlaI GCGC 6 cut(s) 31, 33, 72, 476, 540, 738
GluI GCNGC 4 cut(s) 69, 267, 347, 849
GsaI CCCAGC 2 cut(s) 824, 848
HaeII RGCGCY 1 cut(s) 74
HaeIII GGCC 1 cut(s) 651
HapII CCGG 6 cut(s) 19, 105, 156, 187, 205, 247
HhaI GCGC 6 cut(s) 32, 34, 73, 477, 541, 739
Hin1II CATG 6 cut(s) 102, 154, 238, 481, 676, 763
Hin6I GCGC 6 cut(s) 30, 32, 71, 475, 539, 737
HinP1I GCGC 6 cut(s) 30, 32, 71, 475, 539, 737
HincII GTYRAC 2 cut(s) 288, 598
HindII GTYRAC 2 cut(s) 288, 598
HindIII AAGCTT 1 cut(s) 434
HinfI GANTC 4 cut(s) 7, 167, 213, 271
HpaII CCGG 6 cut(s) 19, 105, 156, 187, 205, 247
HphI GGTGA 2 cut(s) 70, 425
Hpy166II GTNNAC 2 cut(s) 288, 598
Hpy188I TCNGA 6 cut(s) 256, 276, 379, 507, 547, 711
Hpy188III TCNNGA 5 cut(s) 210, 229, 341, 407, 427
Hpy8I GTNNAC 2 cut(s) 288, 598
HpyAV CCTTC 4 cut(s) 151, 404, 712, 761
HpyCH4III ACNGT 2 cut(s) 311, 494
HpyCH4IV ACGT 3 cut(s) 92, 441, 643
HpyF10VI GCNNNNNNNGC 2 cut(s) 19, 581
HpyF3I CTNAG 2 cut(s) 342, 482
HpySE526I ACGT 3 cut(s) 92, 441, 643
Hsp92II CATG 6 cut(s) 102, 154, 238, 481, 676, 763
HspAI GCGC 6 cut(s) 30, 32, 71, 475, 539, 737
Ksp22I TGATCA 2 cut(s) 75, 423
KspI CCGCGG 1 cut(s) 449
Kzo9I GATC 8 cut(s) 75, 251, 423, 502, 589, 706, 721, 756
LmnI GCTCC 6 cut(s) 107, 143, 207, 223, 298, 770
Lsp1109I GCAGC 3 cut(s) 253, 333, 835
LweI GCATC 4 cut(s) 253, 538, 562, 796
MabI ACCWGGT 1 cut(s) 613
MaeI CTAG 1 cut(s) 576
MaeII ACGT 3 cut(s) 92, 441, 643
MaeIII GTNAC 2 cut(s) 107, 781
MalI GATC 8 cut(s) 77, 253, 425, 504, 591, 708, 723, 758
MboI GATC 8 cut(s) 75, 251, 423, 502, 589, 706, 721, 756
MboII GAAGA 5 cut(s) 269, 272, 581, 651, 868
MfeI CAATTG 1 cut(s) 59
MflI RGATCY 3 cut(s) 251, 589, 721
MhlI GDGCHC 2 cut(s) 42, 228
MluCI AATT 3 cut(s) 59, 381, 391
MlyI GAGTC 3 cut(s) 16, 176, 207
MnlI CCTC 6 cut(s) 448, 477, 547, 574, 653, 757
MseI TTAA 2 cut(s) 44, 489
MspA1I CMGCKG 3 cut(s) 448, 824, 848
MspI CCGG 6 cut(s) 19, 105, 156, 187, 205, 247
MspR9I CCNGG 2 cut(s) 247, 615
MunI CAATTG 1 cut(s) 59
MvaI CCWGG 1 cut(s) 615
MvnI CGCG 3 cut(s) 32, 34, 448
MwoI GCNNNNNNNGC 2 cut(s) 19, 581
NciI CCSGG 1 cut(s) 247
NdeII GATC 8 cut(s) 75, 251, 423, 502, 589, 706, 721, 756
NlaIII CATG 6 cut(s) 102, 154, 238, 481, 676, 763
NlaIV GGNNCC 5 cut(s) 240, 534, 591, 723, 772
NmeAIII GCCGAG 1 cut(s) 577
NmuCI GTSAC 1 cut(s) 781
NsbI TGCGCA 1 cut(s) 476
NspI RCATGY 3 cut(s) 102, 481, 676
NspV TTCGAA 1 cut(s) 788
PaeI GCATGC 2 cut(s) 102, 481
PauI GCGCGC 1 cut(s) 30
PciI ACATGT 1 cut(s) 672
PcsI WCGNNNNNNNCGW 2 cut(s) 171, 649
PfeI GAWTC 1 cut(s) 271
PkrI GCNGC 4 cut(s) 70, 268, 348, 850
PleI GAGTC 3 cut(s) 15, 175, 207
PpsI GAGTC 3 cut(s) 15, 175, 207
PscI ACATGT 1 cut(s) 672
Psp124BI GAGCTC 2 cut(s) 42, 228
Psp6I CCWGG 1 cut(s) 613
PspEI GGTNACC 1 cut(s) 107
PspFI CCCAGC 2 cut(s) 820, 844
PspGI CCWGG 1 cut(s) 613
PspN4I GGNNCC 5 cut(s) 240, 534, 591, 723, 772
PspPI GGNCC 3 cut(s) 207, 238, 404
PsuI RGATCY 3 cut(s) 251, 589, 721
PteI GCGCGC 1 cut(s) 30
PvuII CAGCTG 2 cut(s) 824, 848
RsaI GTAC 2 cut(s) 308, 689
RsaNI GTAC 2 cut(s) 307, 688
SacI GAGCTC 2 cut(s) 42, 228
SacII CCGCGG 1 cut(s) 449
SaqAI TTAA 2 cut(s) 44, 489
SatI GCNGC 4 cut(s) 69, 267, 347, 849
Sau3AI GATC 8 cut(s) 75, 251, 423, 502, 589, 706, 721, 756
Sau96I GGNCC 3 cut(s) 207, 238, 404
SchI GAGTC 3 cut(s) 16, 176, 207
ScrFI CCNGG 2 cut(s) 247, 615
SduI GDGCHC 2 cut(s) 42, 228
SexAI ACCWGGT 1 cut(s) 613
SfaNI GCATC 4 cut(s) 253, 538, 562, 796
Sfr303I CCGCGG 1 cut(s) 449
SfuI TTCGAA 1 cut(s) 788
SgrBI CCGCGG 1 cut(s) 449
SinI GGWCC 3 cut(s) 207, 238, 404
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
SphI GCATGC 2 cut(s) 102, 481
Sse9I AATT 3 cut(s) 59, 381, 391
SsiI CCGC 6 cut(s) 68, 86, 190, 217, 446, 448
SspMI CTAG 1 cut(s) 576
SstI GAGCTC 2 cut(s) 42, 228
StyD4I CCNGG 2 cut(s) 245, 613
StyI CCWWGG 1 cut(s) 296
TaaI ACNGT 2 cut(s) 311, 494
TaiI ACGT 3 cut(s) 95, 444, 646
TaqI TCGA 3 cut(s) 174, 389, 788
TasI AATT 3 cut(s) 59, 381, 391
TatI WGTACW 1 cut(s) 306
TauI GCSGC 1 cut(s) 71
TfiI GAWTC 1 cut(s) 271
Tru1I TTAA 2 cut(s) 44, 489
Tru9I TTAA 2 cut(s) 44, 489
TscAI CASTG 1 cut(s) 140
TseFI GTSAC 1 cut(s) 781
TseI GCWGC 3 cut(s) 266, 346, 848
Tsp45I GTSAC 1 cut(s) 781
TspDTI ATGAA 4 cut(s) 111, 536, 582, 595
TspRI CASTG 1 cut(s) 140
VpaK11BI GGWCC 3 cut(s) 207, 238, 404
XapI RAATTY 2 cut(s) 381, 391
XceI RCATGY 3 cut(s) 102, 481, 676
XspI CTAG 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.