Rroxscaffold_5G00385550

5-formyltetrahydrofolate cyclo-ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0005536
Physical Location & Seq
Forward (+)
64596229 .. 64598414
2186 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00385550.1

Sequence Viewer

Length: 879 bp
ATGTTGAGTCACCACCACCGGCAAGCCAGCGCGCGTGAGCTCATAAACACGGCAACACAAGTGGTGGCGGCGCTGATCACCCACCCGCACACGTTAGCATCCTCCGGTAACCATCGCCTTTCATCCCAATCTCACTGCTCCTTCGCCACTATGACCGGAAACGACGAGTCGCTCGAAGCTATATTCCGGCAGAAGCACTCGCTCCGGTCCAGAATCCGCAAGGCTCTCAAGAACATGGACCCGACCTGGAGATCCGAAGAAGATGCTGCAATTCAGAGCATTGTGTTGACTGCTCCATGGTTCAAGTACAGTCAAGAAGGTGATGTGCTGATCAGGAGAAAGCTTTACGTTCCGCGGGTTGAGGATAGGAATAGTAATATGCGCATGCTGAGGATTAACAGTGTTGGTGATCTGATTGTGAAGTCTATGAACGTTTTGGAACCTGCGATTTCGGATGCCGAGGGAAACAATATGAAGATGGGAATATTTCCCCATCTCTTGCGAAGAGTTGGATTTATGCATTTGAAAGTCGAAATGCAGGAAAGCCTACTGCATTTCTCTATGGAGGCAAAGGATCCAGTTGACTTGTTCATCCTACCTGGTCTTGCTTTTGACAGATGTGGAAGACGTTTAGGCCGTAGTGGAGGGTGGGTTGATACATCTTATTCTAGTTATTATGACATGTTCCTAAAGAAGTACCAAAAGCTCGCAAAAGATCGGAAATGGAAGGATCCCCTCTGTGTTGCGCTATCATATTCAATACAGATAGTGGAGGAAGGAGCCATACCTGTCACTTCGAATGATGTTTCTGTGGATGCTCTTGTATCCCCAGCTGGTTGTGTTCCAATAAGCCCAGCTGCTTGGAAGAGATGTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00670 ko01100 map00670 map01100
Pfam Domains
Protein Families
5-FTHF_cyc-lig

Protein Analysis

292

Amino Acids

32.91

Weight (kDa)

9.28

Isoelectric Point (pI)

42.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5-FTHF_cyc-lig PF01812 65 - 277 9.2e-15 5-formyltetrahydrofolate cyclo-ligase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 383
Acc36I ACCTGC 1 cut(s) 451
AccII CGCG 3 cut(s) 32, 34, 355
AciI CCGC 5 cut(s) 68, 86, 217, 353, 355
AclI AACGTT 1 cut(s) 432
AclWI GGATC 5 cut(s) 246, 569, 582, 725, 738
AfaI GTAC 2 cut(s) 308, 698
AflIII ACRYGT 2 cut(s) 90, 681
AgsI TTSAA 3 cut(s) 304, 526, 759
AjnI CCWGG 2 cut(s) 245, 598
AluBI AGCT 6 cut(s) 40, 179, 343, 706, 833, 857
AluI AGCT 6 cut(s) 40, 179, 343, 706, 833, 857
Alw21I GWGCWC 1 cut(s) 42
AlwI GGATC 5 cut(s) 246, 569, 582, 725, 738
AoxI GGCC 1 cut(s) 634
ApeKI GCWGC 2 cut(s) 266, 857
AspLEI GCGC 5 cut(s) 32, 34, 73, 384, 748
AspS9I GGNCC 2 cut(s) 207, 238
AsuHPI GGTGA 3 cut(s) 70, 332, 419
AsuII TTCGAA 1 cut(s) 797
AvaII GGWCC 2 cut(s) 207, 238
BamHI GGATCC 2 cut(s) 574, 730
BanII GRGCYC 1 cut(s) 42
BarI GAAGNNNNNNTAC 2 cut(s) 768, 800
BbsI GAAGAC 1 cut(s) 631
Bbv12I GWGCWC 1 cut(s) 42
BbvCI CCTCAGC 1 cut(s) 389
BbvI GCAGC 2 cut(s) 253, 844
BccI CCATC 3 cut(s) 120, 472, 501
BceAI ACGGC 2 cut(s) 66, 621
BcgI CGANNNNNNTGC 2 cut(s) 245, 279
BciT130I CCWGG 2 cut(s) 247, 600
BciVI GTATCC 1 cut(s) 835
BclI TGATCA 2 cut(s) 75, 330
BfaI CTAG 1 cut(s) 669
BfoI RGCGCY 1 cut(s) 74
BfuAI ACCTGC 1 cut(s) 451
BfuI GTATCC 1 cut(s) 835
BisI GCNGC 3 cut(s) 69, 267, 858
BlsI GCNGC 3 cut(s) 70, 268, 859
Bme1390I CCNGG 2 cut(s) 247, 600
Bme18I GGWCC 2 cut(s) 207, 238
BmgT120I GGNCC 2 cut(s) 207, 238
BmiI GGNNCC 5 cut(s) 240, 441, 576, 732, 781
BmrFI CCNGG 2 cut(s) 247, 600
BmsI GCATC 4 cut(s) 107, 253, 445, 805
BpiI GAAGAC 1 cut(s) 631
BpmI CTGGAG 1 cut(s) 268
Bpu10I CCTNAGC 1 cut(s) 389
Bpu14I TTCGAA 1 cut(s) 797
BpuEI CTTGAG 1 cut(s) 212
BsaJI CCNNGG 3 cut(s) 296, 353, 459
BsaWI WCCGGW 3 cut(s) 104, 155, 204
BsaXI ACNNNNNCTCC 2 cut(s) 636, 666
Bse118I RCCGGY 1 cut(s) 18
Bse1I ACTGG 1 cut(s) 578
BseBI CCWGG 2 cut(s) 247, 600
BseDI CCNNGG 3 cut(s) 296, 353, 459
BseGI GGATG 5 cut(s) 98, 122, 460, 591, 820
BseMII CTCAG 1 cut(s) 380
BseNI ACTGG 1 cut(s) 578
BsePI GCGCGC 1 cut(s) 30
BseXI GCAGC 2 cut(s) 253, 844
BseYI CCCAGC 2 cut(s) 829, 853
Bsh1236I CGCG 3 cut(s) 32, 34, 355
BshFI GGCC 1 cut(s) 636
BsiHKAI GWGCWC 1 cut(s) 42
BsiSI CCGG 5 cut(s) 19, 105, 156, 187, 205
BsnI GGCC 1 cut(s) 636
Bsp119I TTCGAA 1 cut(s) 797
Bsp1286I GDGCHC 1 cut(s) 42
Bsp143I GATC 7 cut(s) 75, 251, 330, 409, 574, 715, 730
Bsp19I CCATGG 1 cut(s) 296
BspACI CCGC 5 cut(s) 68, 86, 217, 353, 355
BspANI GGCC 1 cut(s) 636
BspCNI CTCAG 1 cut(s) 381
BspFNI CGCG 3 cut(s) 32, 34, 355
BspLI GGNNCC 5 cut(s) 240, 441, 576, 732, 781
BspMI ACCTGC 1 cut(s) 451
BspPI GGATC 5 cut(s) 246, 569, 582, 725, 738
BspT104I TTCGAA 1 cut(s) 797
BsrFI RCCGGY 1 cut(s) 18
BsrI ACTGG 1 cut(s) 578
BssAI RCCGGY 1 cut(s) 18
BssECI CCNNGG 3 cut(s) 296, 353, 459
BssHII GCGCGC 1 cut(s) 30
BssMI GATC 7 cut(s) 75, 251, 330, 409, 574, 715, 730
BssT1I CCWWGG 1 cut(s) 296
Bst2UI CCWGG 2 cut(s) 247, 600
Bst4CI ACNGT 2 cut(s) 311, 401
Bst6I CTCTTC 2 cut(s) 499, 860
BstBI TTCGAA 1 cut(s) 797
BstC8I GCNNGC 5 cut(s) 24, 28, 32, 386, 708
BstDEI CTNAG 1 cut(s) 389
BstDSI CCRYGG 2 cut(s) 296, 353
BstEII GGTNACC 1 cut(s) 107
BstF5I GGATG 5 cut(s) 98, 122, 460, 591, 820
BstFNI CGCG 3 cut(s) 32, 34, 355
BstH2I RGCGCY 1 cut(s) 74
BstHHI GCGC 5 cut(s) 32, 34, 73, 384, 748
BstKTI GATC 7 cut(s) 78, 254, 333, 412, 577, 718, 733
BstMBI GATC 7 cut(s) 75, 251, 330, 409, 574, 715, 730
BstNI CCWGG 2 cut(s) 247, 600
BstNSI RCATGY 2 cut(s) 388, 685
BstPI GGTNACC 1 cut(s) 107
BstSCI CCNGG 2 cut(s) 245, 598
BstUI CGCG 3 cut(s) 32, 34, 355
BstV1I GCAGC 2 cut(s) 253, 844
BstV2I GAAGAC 1 cut(s) 631
BstX2I RGATCY 3 cut(s) 251, 574, 730
BstXI CCANNNNNNTGG 1 cut(s) 861
BstYI RGATCY 3 cut(s) 251, 574, 730
BsuI GTATCC 1 cut(s) 835
BsuRI GGCC 1 cut(s) 636
BtgI CCRYGG 2 cut(s) 296, 353
BtgZI GCGATG 1 cut(s) 98
BtsCI GGATG 5 cut(s) 98, 122, 460, 591, 820
BtsI GCAGTG 1 cut(s) 133
BtsIMutI CAGTG 2 cut(s) 133, 406
BveI ACCTGC 1 cut(s) 451
Cac8I GCNNGC 5 cut(s) 24, 28, 32, 386, 708
CfoI GCGC 5 cut(s) 32, 34, 73, 384, 748
Cfr10I RCCGGY 1 cut(s) 18
Cfr13I GGNCC 2 cut(s) 207, 238
Cfr42I CCGCGG 1 cut(s) 356
CsiI ACCWGGT 1 cut(s) 598
Csp6I GTAC 2 cut(s) 307, 697
CspCI CAANNNNNGTGG 2 cut(s) 42, 77
CviAII CATG 4 cut(s) 235, 297, 385, 682
CviQI GTAC 2 cut(s) 307, 697
DdeI CTNAG 1 cut(s) 389
DpnI GATC 7 cut(s) 77, 253, 332, 411, 576, 717, 732
DpnII GATC 7 cut(s) 75, 251, 330, 409, 574, 715, 730
Eam1104I CTCTTC 2 cut(s) 499, 860
EarI CTCTTC 2 cut(s) 499, 860
Ecl136II GAGCTC 1 cut(s) 40
Eco130I CCWWGG 1 cut(s) 296
Eco24I GRGCYC 1 cut(s) 42
Eco47I GGWCC 2 cut(s) 207, 238
Eco53kI GAGCTC 1 cut(s) 40
Eco91I GGTNACC 1 cut(s) 107
EcoICRI GAGCTC 1 cut(s) 40
EcoO65I GGTNACC 1 cut(s) 107
EcoRII CCWGG 2 cut(s) 245, 598
EcoT14I CCWWGG 1 cut(s) 296
EcoT22I ATGCAT 1 cut(s) 522
EcoT38I GRGCYC 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 296
FaeI CATG 4 cut(s) 238, 300, 388, 685
FatI CATG 4 cut(s) 234, 296, 384, 681
FauI CCCGC 2 cut(s) 93, 348
FbaI TGATCA 2 cut(s) 75, 330
Fnu4HI GCNGC 3 cut(s) 69, 267, 858
FokI GGATG 5 cut(s) 85, 109, 467, 578, 827
FriOI GRGCYC 1 cut(s) 42
Fsp4HI GCNGC 3 cut(s) 69, 267, 858
FspAI RTGCGCAY 1 cut(s) 383
FspBI CTAG 1 cut(s) 669
FspI TGCGCA 1 cut(s) 383
GlaI GCGC 5 cut(s) 31, 33, 72, 383, 747
GluI GCNGC 3 cut(s) 69, 267, 858
GsaI CCCAGC 2 cut(s) 833, 857
GsuI CTGGAG 1 cut(s) 268
HaeII RGCGCY 1 cut(s) 74
HaeIII GGCC 1 cut(s) 636
HapII CCGG 5 cut(s) 19, 105, 156, 187, 205
HhaI GCGC 5 cut(s) 32, 34, 73, 384, 748
Hin1II CATG 4 cut(s) 238, 300, 388, 685
Hin6I GCGC 5 cut(s) 30, 32, 71, 382, 746
HinP1I GCGC 5 cut(s) 30, 32, 71, 382, 746
HincII GTYRAC 2 cut(s) 288, 583
HindII GTYRAC 2 cut(s) 288, 583
HindIII AAGCTT 1 cut(s) 341
HinfI GANTC 3 cut(s) 7, 167, 213
HpaII CCGG 5 cut(s) 19, 105, 156, 187, 205
HphI GGTGA 3 cut(s) 70, 332, 419
Hpy166II GTNNAC 2 cut(s) 288, 583
Hpy188I TCNGA 5 cut(s) 256, 276, 414, 454, 720
Hpy188III TCNNGA 4 cut(s) 210, 229, 314, 334
Hpy8I GTNNAC 2 cut(s) 288, 583
Hpy99I CGWCG 1 cut(s) 167
HpyAV CCTTC 4 cut(s) 151, 311, 721, 770
HpyCH4III ACNGT 2 cut(s) 311, 401
HpyCH4IV ACGT 4 cut(s) 92, 348, 432, 628
HpyCH4V TGCA 4 cut(s) 269, 520, 538, 553
HpyF3I CTNAG 1 cut(s) 389
HpySE526I ACGT 4 cut(s) 92, 348, 432, 628
Hsp92II CATG 4 cut(s) 238, 300, 388, 685
HspAI GCGC 5 cut(s) 30, 32, 71, 382, 746
Ksp22I TGATCA 2 cut(s) 75, 330
KspI CCGCGG 1 cut(s) 356
Kzo9I GATC 7 cut(s) 75, 251, 330, 409, 574, 715, 730
LmnI GCTCC 4 cut(s) 143, 207, 298, 779
Lsp1109I GCAGC 2 cut(s) 253, 844
LweI GCATC 4 cut(s) 107, 253, 445, 805
MabI ACCWGGT 1 cut(s) 598
MaeI CTAG 1 cut(s) 669
MaeII ACGT 4 cut(s) 92, 348, 432, 628
MaeIII GTNAC 3 cut(s) 8, 107, 790
MalI GATC 7 cut(s) 77, 253, 332, 411, 576, 717, 732
MboI GATC 7 cut(s) 75, 251, 330, 409, 574, 715, 730
MboII GAAGA 6 cut(s) 269, 272, 487, 516, 636, 877
MflI RGATCY 3 cut(s) 251, 574, 730
MhlI GDGCHC 1 cut(s) 42
MluCI AATT 1 cut(s) 270
MlyI GAGTC 2 cut(s) 16, 176
MmeI TCCRAC 1 cut(s) 490
MnlI CCTC 8 cut(s) 112, 355, 384, 454, 559, 638, 746, 766
Mph1103I ATGCAT 1 cut(s) 522
MseI TTAA 1 cut(s) 396
MspA1I CMGCKG 3 cut(s) 355, 833, 857
MspI CCGG 5 cut(s) 19, 105, 156, 187, 205
MspR9I CCNGG 2 cut(s) 247, 600
MvaI CCWGG 2 cut(s) 247, 600
MvnI CGCG 3 cut(s) 32, 34, 355
NcoI CCATGG 1 cut(s) 296
NdeII GATC 7 cut(s) 75, 251, 330, 409, 574, 715, 730
NlaIII CATG 4 cut(s) 238, 300, 388, 685
NlaIV GGNNCC 5 cut(s) 240, 441, 576, 732, 781
NmeAIII GCCGAG 1 cut(s) 484
NmuCI GTSAC 2 cut(s) 8, 790
NsbI TGCGCA 1 cut(s) 383
NsiI ATGCAT 1 cut(s) 522
NspI RCATGY 2 cut(s) 388, 685
NspV TTCGAA 1 cut(s) 797
PaeI GCATGC 1 cut(s) 388
PauI GCGCGC 1 cut(s) 30
PciI ACATGT 1 cut(s) 681
PcsI WCGNNNNNNNCGW 2 cut(s) 171, 634
PfeI GAWTC 1 cut(s) 213
PkrI GCNGC 3 cut(s) 70, 268, 859
PleI GAGTC 2 cut(s) 15, 175
PpsI GAGTC 2 cut(s) 15, 175
PscI ACATGT 1 cut(s) 681
Psp124BI GAGCTC 1 cut(s) 42
Psp1406I AACGTT 1 cut(s) 432
Psp6I CCWGG 2 cut(s) 245, 598
PspEI GGTNACC 1 cut(s) 107
PspFI CCCAGC 2 cut(s) 829, 853
PspGI CCWGG 2 cut(s) 245, 598
PspN4I GGNNCC 5 cut(s) 240, 441, 576, 732, 781
PspPI GGNCC 2 cut(s) 207, 238
PsuI RGATCY 3 cut(s) 251, 574, 730
PteI GCGCGC 1 cut(s) 30
PvuII CAGCTG 2 cut(s) 833, 857
RsaI GTAC 2 cut(s) 308, 698
RsaNI GTAC 2 cut(s) 307, 697
SacI GAGCTC 1 cut(s) 42
SacII CCGCGG 1 cut(s) 356
SaqAI TTAA 1 cut(s) 396
SatI GCNGC 3 cut(s) 69, 267, 858
Sau3AI GATC 7 cut(s) 75, 251, 330, 409, 574, 715, 730
Sau96I GGNCC 2 cut(s) 207, 238
SchI GAGTC 2 cut(s) 16, 176
ScrFI CCNGG 2 cut(s) 247, 600
SduI GDGCHC 1 cut(s) 42
SexAI ACCWGGT 1 cut(s) 598
SfaNI GCATC 4 cut(s) 107, 253, 445, 805
Sfr303I CCGCGG 1 cut(s) 356
SfuI TTCGAA 1 cut(s) 797
SgrBI CCGCGG 1 cut(s) 356
SinI GGWCC 2 cut(s) 207, 238
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
SphI GCATGC 1 cut(s) 388
Sse9I AATT 1 cut(s) 270
SsiI CCGC 5 cut(s) 68, 86, 217, 353, 355
SspI AATATT 1 cut(s) 486
SspMI CTAG 1 cut(s) 669
SstI GAGCTC 1 cut(s) 42
StyD4I CCNGG 2 cut(s) 245, 598
StyI CCWWGG 1 cut(s) 296
TaaI ACNGT 2 cut(s) 311, 401
TaiI ACGT 4 cut(s) 95, 351, 435, 631
TaqI TCGA 3 cut(s) 174, 531, 797
TasI AATT 1 cut(s) 270
TatI WGTACW 1 cut(s) 306
TauI GCSGC 1 cut(s) 71
TfiI GAWTC 1 cut(s) 213
Tru1I TTAA 1 cut(s) 396
Tru9I TTAA 1 cut(s) 396
TscAI CASTG 2 cut(s) 140, 406
TseFI GTSAC 2 cut(s) 8, 790
TseI GCWGC 2 cut(s) 266, 857
Tsp45I GTSAC 2 cut(s) 8, 790
TspDTI ATGAA 4 cut(s) 111, 443, 488, 580
TspRI CASTG 2 cut(s) 140, 406
VpaK11BI GGWCC 2 cut(s) 207, 238
XceI RCATGY 2 cut(s) 388, 685
XspI CTAG 1 cut(s) 669
Zsp2I ATGCAT 1 cut(s) 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.