Rw4G035900

5-formyltetrahydrofolate cyclo-ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0005536
Physical Location & Seq
Forward (+)
62753045 .. 62755228
2184 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G035900.1

Sequence Viewer

Length: 870 bp
ATGTTGAGTCACCACCACCGGCAATCCAGCGCGCGTGAGCTCTTAAACACGGCAACGCAAGTGGCGGCGGCGCTGATCACCCACCCGCACACGTTAGCATGCTCCGGTAACCAACGCCTTTCATCCCAATCTCACTGCTCCTTCGCCACCATGACCGGAAAGGACGAGTCGCTCGAAGCTATATTCCGGCGGAAGCACTCGCTCCGGTCCAGACTCCGCAAGGAGCTCAAGAACATGGACCCGACCCGGAGATCCGAAGAAGATGCTGCGATTCAGAGCATTGTGTCGACTGCTCCATGGTTCAAGTACAGTAAGAGTATATGTGCTTATATAAGCTCTCCTGAGCTGCGAGAAGTAGATACTTCAAGGATTGTGTCAGAAATTCTATCGAATTCGGGCAATGGTCAAGAAGGTGATGTGCTGATCAGGAGAAAGCTTTACGTTCCGCGGGTTGAGGATAGGAATAGTAATATGCGCATGCTGAGGATTAACGGTGTTGGTGATCTGATTGTGAAGTCTATGAACGTTTTGGAACCTGCGCTTTCGGATGCCGAGGGGAAACAATATGAAGATGCTATGGAGGCAAAGGATCCAGTTGACTTGTTCATCCTACCTGGTCTTGCTTTTGACAGATGTGGAAGACGTTTAGGCCGTAGTGGAGGTTATTATGACATGTTCCTAAAGAGGTACCAAAAGCTTGCAAAAGATCGGAAATGGAAGGATCCCCTCTGTGTTGCGCTATCATATTCAATACAGATCGTGGAGGAAGGAGCCATACCTGTCACTTCGAATGATGTTTCTGTGGATGCTCTTGTATCCCCAGCTGGTTTTGTTCCAATAAGCCCAGCTGCTTGGAAGAGATGTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00670 ko01100 map00670 map01100
Pfam Domains
Protein Families
5-FTHF_cyc-lig

Protein Analysis

289

Amino Acids

32.28

Weight (kDa)

9.09

Isoelectric Point (pI)

49.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5-FTHF_cyc-lig PF01812 65 - 274 1.9e-27 5-formyltetrahydrofolate cyclo-ligase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 476
Acc36I ACCTGC 1 cut(s) 544
Acc65I GGTACC 1 cut(s) 687
AccB1I GGYRCC 1 cut(s) 687
AccI GTMKAC 1 cut(s) 287
AccII CGCG 3 cut(s) 32, 34, 448
AciI CCGC 7 cut(s) 65, 68, 86, 190, 217, 446, 448
AclI AACGTT 1 cut(s) 525
AclWI GGATC 5 cut(s) 246, 584, 597, 716, 729
AcsI RAATTY 2 cut(s) 381, 391
AfaI GTAC 2 cut(s) 308, 689
AflIII ACRYGT 2 cut(s) 90, 672
AgsI TTSAA 3 cut(s) 304, 366, 750
AjnI CCWGG 1 cut(s) 613
AluBI AGCT 9 cut(s) 40, 179, 226, 336, 346, 436, 697, 824, 848
AluI AGCT 9 cut(s) 40, 179, 226, 336, 346, 436, 697, 824, 848
Alw21I GWGCWC 2 cut(s) 42, 228
AlwI GGATC 5 cut(s) 246, 584, 597, 716, 729
AoxI GGCC 1 cut(s) 649
ApeKI GCWGC 3 cut(s) 266, 346, 848
ApoI RAATTY 2 cut(s) 381, 391
Asp718I GGTACC 1 cut(s) 687
AspLEI GCGC 6 cut(s) 32, 34, 73, 477, 541, 739
AspS9I GGNCC 2 cut(s) 207, 238
AsuC2I CCSGG 1 cut(s) 247
AsuHPI GGTGA 3 cut(s) 70, 425, 512
AsuII TTCGAA 1 cut(s) 788
AvaII GGWCC 2 cut(s) 207, 238
BamHI GGATCC 2 cut(s) 589, 721
BanI GGYRCC 1 cut(s) 687
BanII GRGCYC 2 cut(s) 42, 228
BarI GAAGNNNNNNTAC 2 cut(s) 759, 791
BbsI GAAGAC 1 cut(s) 646
Bbv12I GWGCWC 2 cut(s) 42, 228
BbvCI CCTCAGC 1 cut(s) 482
BbvI GCAGC 3 cut(s) 253, 333, 835
BceAI ACGGC 2 cut(s) 66, 636
BcgI CGANNNNNNTGC 2 cut(s) 245, 279
BciT130I CCWGG 1 cut(s) 615
BciVI GTATCC 1 cut(s) 826
BclI TGATCA 2 cut(s) 75, 423
BcnI CCSGG 1 cut(s) 247
BfoI RGCGCY 1 cut(s) 74
BfuAI ACCTGC 1 cut(s) 544
BfuI GTATCC 1 cut(s) 826
BisI GCNGC 5 cut(s) 66, 69, 267, 347, 849
BlsI GCNGC 5 cut(s) 67, 70, 268, 348, 850
Bme1390I CCNGG 2 cut(s) 247, 615
Bme18I GGWCC 2 cut(s) 207, 238
BmgT120I GGNCC 2 cut(s) 207, 238
BmiI GGNNCC 6 cut(s) 240, 534, 591, 689, 723, 772
BmrFI CCNGG 2 cut(s) 247, 615
BmsI GCATC 4 cut(s) 253, 538, 562, 796
BpiI GAAGAC 1 cut(s) 646
Bpu10I CCTNAGC 2 cut(s) 342, 482
Bpu14I TTCGAA 1 cut(s) 788
BpuEI CTTGAG 1 cut(s) 212
BpuMI CCSGG 1 cut(s) 247
BsaJI CCNNGG 3 cut(s) 296, 446, 552
BsaWI WCCGGW 3 cut(s) 104, 155, 204
Bse118I RCCGGY 1 cut(s) 18
Bse1I ACTGG 1 cut(s) 593
Bse3DI GCAATG 1 cut(s) 406
BseBI CCWGG 1 cut(s) 615
BseDI CCNNGG 3 cut(s) 296, 446, 552
BseGI GGATG 4 cut(s) 122, 553, 606, 811
BseMI GCAATG 1 cut(s) 406
BseMII CTCAG 2 cut(s) 333, 473
BseNI ACTGG 1 cut(s) 593
BsePI GCGCGC 1 cut(s) 30
BseXI GCAGC 3 cut(s) 253, 333, 835
BseYI CCCAGC 2 cut(s) 820, 844
Bsh1236I CGCG 3 cut(s) 32, 34, 448
BshFI GGCC 1 cut(s) 651
BshNI GGYRCC 1 cut(s) 687
BsiHKAI GWGCWC 2 cut(s) 42, 228
BsiSI CCGG 6 cut(s) 19, 105, 156, 187, 205, 247
BsnI GGCC 1 cut(s) 651
Bsp119I TTCGAA 1 cut(s) 788
Bsp1286I GDGCHC 2 cut(s) 42, 228
Bsp143I GATC 8 cut(s) 75, 251, 423, 502, 589, 706, 721, 756
Bsp19I CCATGG 1 cut(s) 296
BspACI CCGC 7 cut(s) 65, 68, 86, 190, 217, 446, 448
BspANI GGCC 1 cut(s) 651
BspCNI CTCAG 2 cut(s) 334, 474
BspFNI CGCG 3 cut(s) 32, 34, 448
BspLI GGNNCC 6 cut(s) 240, 534, 591, 689, 723, 772
BspMI ACCTGC 1 cut(s) 544
BspPI GGATC 5 cut(s) 246, 584, 597, 716, 729
BspT104I TTCGAA 1 cut(s) 788
BspT107I GGYRCC 1 cut(s) 687
BsrDI GCAATG 1 cut(s) 406
BsrFI RCCGGY 1 cut(s) 18
BsrI ACTGG 1 cut(s) 593
BssAI RCCGGY 1 cut(s) 18
BssECI CCNNGG 3 cut(s) 296, 446, 552
BssHII GCGCGC 1 cut(s) 30
BssMI GATC 8 cut(s) 75, 251, 423, 502, 589, 706, 721, 756
BssT1I CCWWGG 1 cut(s) 296
Bst2UI CCWGG 1 cut(s) 615
Bst4CI ACNGT 2 cut(s) 311, 494
Bst6I CTCTTC 1 cut(s) 851
BstBI TTCGAA 1 cut(s) 788
BstC8I GCNNGC 4 cut(s) 32, 100, 479, 699
BstDEI CTNAG 2 cut(s) 342, 482
BstDSI CCRYGG 2 cut(s) 296, 446
BstEII GGTNACC 1 cut(s) 107
BstF5I GGATG 4 cut(s) 122, 553, 606, 811
BstFNI CGCG 3 cut(s) 32, 34, 448
BstH2I RGCGCY 1 cut(s) 74
BstHHI GCGC 6 cut(s) 32, 34, 73, 477, 541, 739
BstKTI GATC 8 cut(s) 78, 254, 426, 505, 592, 709, 724, 759
BstMBI GATC 8 cut(s) 75, 251, 423, 502, 589, 706, 721, 756
BstMWI GCNNNNNNNGC 1 cut(s) 581
BstNI CCWGG 1 cut(s) 615
BstNSI RCATGY 3 cut(s) 102, 481, 676
BstPI GGTNACC 1 cut(s) 107
BstSCI CCNGG 2 cut(s) 245, 613
BstUI CGCG 3 cut(s) 32, 34, 448
BstV1I GCAGC 3 cut(s) 253, 333, 835
BstV2I GAAGAC 1 cut(s) 646
BstX2I RGATCY 3 cut(s) 251, 589, 721
BstXI CCANNNNNNTGG 1 cut(s) 852
BstYI RGATCY 3 cut(s) 251, 589, 721
BsuI GTATCC 1 cut(s) 826
BsuRI GGCC 1 cut(s) 651
BtgI CCRYGG 2 cut(s) 296, 446
BtsCI GGATG 4 cut(s) 122, 553, 606, 811
BtsI GCAGTG 1 cut(s) 133
BtsIMutI CAGTG 1 cut(s) 133
BveI ACCTGC 1 cut(s) 544
Cac8I GCNNGC 4 cut(s) 32, 100, 479, 699
CfoI GCGC 6 cut(s) 32, 34, 73, 477, 541, 739
Cfr10I RCCGGY 1 cut(s) 18
Cfr13I GGNCC 2 cut(s) 207, 238
Cfr42I CCGCGG 1 cut(s) 449
CsiI ACCWGGT 1 cut(s) 613
Csp6I GTAC 2 cut(s) 307, 688
CspCI CAANNNNNGTGG 2 cut(s) 42, 77
CviAII CATG 6 cut(s) 99, 151, 235, 297, 478, 673
CviQI GTAC 2 cut(s) 307, 688
DdeI CTNAG 2 cut(s) 342, 482
DpnI GATC 8 cut(s) 77, 253, 425, 504, 591, 708, 723, 758
DpnII GATC 8 cut(s) 75, 251, 423, 502, 589, 706, 721, 756
Eam1104I CTCTTC 1 cut(s) 851
EarI CTCTTC 1 cut(s) 851
EciI GGCGGA 1 cut(s) 205
Ecl136II GAGCTC 2 cut(s) 40, 226
Eco130I CCWWGG 1 cut(s) 296
Eco24I GRGCYC 2 cut(s) 42, 228
Eco47I GGWCC 2 cut(s) 207, 238
Eco53kI GAGCTC 2 cut(s) 40, 226
Eco91I GGTNACC 1 cut(s) 107
EcoICRI GAGCTC 2 cut(s) 40, 226
EcoO65I GGTNACC 1 cut(s) 107
EcoRI GAATTC 1 cut(s) 391
EcoRII CCWGG 1 cut(s) 613
EcoT14I CCWWGG 1 cut(s) 296
EcoT38I GRGCYC 2 cut(s) 42, 228
ErhI CCWWGG 1 cut(s) 296
FaeI CATG 6 cut(s) 102, 154, 238, 300, 481, 676
FatI CATG 6 cut(s) 98, 150, 234, 296, 477, 672
FauI CCCGC 2 cut(s) 93, 441
FbaI TGATCA 2 cut(s) 75, 423
FblI GTMKAC 1 cut(s) 287
Fnu4HI GCNGC 5 cut(s) 66, 69, 267, 347, 849
FokI GGATG 4 cut(s) 109, 560, 593, 818
FriOI GRGCYC 2 cut(s) 42, 228
Fsp4HI GCNGC 5 cut(s) 66, 69, 267, 347, 849
FspAI RTGCGCAY 1 cut(s) 476
FspI TGCGCA 1 cut(s) 476
GlaI GCGC 6 cut(s) 31, 33, 72, 476, 540, 738
GluI GCNGC 5 cut(s) 66, 69, 267, 347, 849
GsaI CCCAGC 2 cut(s) 824, 848
HaeII RGCGCY 1 cut(s) 74
HaeIII GGCC 1 cut(s) 651
HapII CCGG 6 cut(s) 19, 105, 156, 187, 205, 247
HhaI GCGC 6 cut(s) 32, 34, 73, 477, 541, 739
Hin1II CATG 6 cut(s) 102, 154, 238, 300, 481, 676
Hin6I GCGC 6 cut(s) 30, 32, 71, 475, 539, 737
HinP1I GCGC 6 cut(s) 30, 32, 71, 475, 539, 737
HincII GTYRAC 2 cut(s) 288, 598
HindII GTYRAC 2 cut(s) 288, 598
HindIII AAGCTT 2 cut(s) 434, 695
HinfI GANTC 4 cut(s) 7, 167, 213, 271
HpaII CCGG 6 cut(s) 19, 105, 156, 187, 205, 247
HphI GGTGA 3 cut(s) 70, 425, 512
Hpy166II GTNNAC 2 cut(s) 288, 598
Hpy188I TCNGA 6 cut(s) 256, 276, 379, 507, 547, 711
Hpy188III TCNNGA 5 cut(s) 210, 229, 341, 407, 427
Hpy8I GTNNAC 2 cut(s) 288, 598
HpyAV CCTTC 4 cut(s) 151, 404, 712, 761
HpyCH4III ACNGT 2 cut(s) 311, 494
HpyCH4IV ACGT 4 cut(s) 92, 441, 525, 643
HpyCH4V TGCA 1 cut(s) 701
HpyF10VI GCNNNNNNNGC 1 cut(s) 581
HpyF3I CTNAG 2 cut(s) 342, 482
HpySE526I ACGT 4 cut(s) 92, 441, 525, 643
Hsp92II CATG 6 cut(s) 102, 154, 238, 300, 481, 676
HspAI GCGC 6 cut(s) 30, 32, 71, 475, 539, 737
KpnI GGTACC 1 cut(s) 691
Ksp22I TGATCA 2 cut(s) 75, 423
KspI CCGCGG 1 cut(s) 449
Kzo9I GATC 8 cut(s) 75, 251, 423, 502, 589, 706, 721, 756
LmnI GCTCC 6 cut(s) 107, 143, 207, 223, 298, 770
Lsp1109I GCAGC 3 cut(s) 253, 333, 835
LweI GCATC 4 cut(s) 253, 538, 562, 796
MabI ACCWGGT 1 cut(s) 613
MaeII ACGT 4 cut(s) 92, 441, 525, 643
MaeIII GTNAC 3 cut(s) 8, 107, 781
MalI GATC 8 cut(s) 77, 253, 425, 504, 591, 708, 723, 758
MboI GATC 8 cut(s) 75, 251, 423, 502, 589, 706, 721, 756
MboII GAAGA 5 cut(s) 269, 272, 581, 651, 868
MflI RGATCY 3 cut(s) 251, 589, 721
MhlI GDGCHC 2 cut(s) 42, 228
MluCI AATT 2 cut(s) 381, 391
MlyI GAGTC 3 cut(s) 16, 176, 207
MnlI CCTC 8 cut(s) 448, 477, 547, 574, 653, 678, 737, 757
MseI TTAA 2 cut(s) 44, 489
MspA1I CMGCKG 3 cut(s) 448, 824, 848
MspI CCGG 6 cut(s) 19, 105, 156, 187, 205, 247
MspR9I CCNGG 2 cut(s) 247, 615
MvaI CCWGG 1 cut(s) 615
MvnI CGCG 3 cut(s) 32, 34, 448
MwoI GCNNNNNNNGC 1 cut(s) 581
NciI CCSGG 1 cut(s) 247
NcoI CCATGG 1 cut(s) 296
NdeII GATC 8 cut(s) 75, 251, 423, 502, 589, 706, 721, 756
NlaIII CATG 6 cut(s) 102, 154, 238, 300, 481, 676
NlaIV GGNNCC 6 cut(s) 240, 534, 591, 689, 723, 772
NmeAIII GCCGAG 1 cut(s) 577
NmuCI GTSAC 2 cut(s) 8, 781
NsbI TGCGCA 1 cut(s) 476
NspI RCATGY 3 cut(s) 102, 481, 676
NspV TTCGAA 1 cut(s) 788
PaeI GCATGC 2 cut(s) 102, 481
PauI GCGCGC 1 cut(s) 30
PciI ACATGT 1 cut(s) 672
PcsI WCGNNNNNNNCGW 2 cut(s) 171, 649
PfeI GAWTC 1 cut(s) 271
PkrI GCNGC 5 cut(s) 67, 70, 268, 348, 850
PleI GAGTC 3 cut(s) 15, 175, 207
PpsI GAGTC 3 cut(s) 15, 175, 207
PscI ACATGT 1 cut(s) 672
Psp124BI GAGCTC 2 cut(s) 42, 228
Psp1406I AACGTT 1 cut(s) 525
Psp6I CCWGG 1 cut(s) 613
PspEI GGTNACC 1 cut(s) 107
PspFI CCCAGC 2 cut(s) 820, 844
PspGI CCWGG 1 cut(s) 613
PspN4I GGNNCC 6 cut(s) 240, 534, 591, 689, 723, 772
PspPI GGNCC 2 cut(s) 207, 238
PsuI RGATCY 3 cut(s) 251, 589, 721
PteI GCGCGC 1 cut(s) 30
PvuII CAGCTG 2 cut(s) 824, 848
RsaI GTAC 2 cut(s) 308, 689
RsaNI GTAC 2 cut(s) 307, 688
SacI GAGCTC 2 cut(s) 42, 228
SacII CCGCGG 1 cut(s) 449
SalI GTCGAC 1 cut(s) 286
SaqAI TTAA 2 cut(s) 44, 489
SatI GCNGC 5 cut(s) 66, 69, 267, 347, 849
Sau3AI GATC 8 cut(s) 75, 251, 423, 502, 589, 706, 721, 756
Sau96I GGNCC 2 cut(s) 207, 238
SchI GAGTC 3 cut(s) 16, 176, 207
ScrFI CCNGG 2 cut(s) 247, 615
SduI GDGCHC 2 cut(s) 42, 228
SexAI ACCWGGT 1 cut(s) 613
SfaNI GCATC 4 cut(s) 253, 538, 562, 796
Sfr303I CCGCGG 1 cut(s) 449
SfuI TTCGAA 1 cut(s) 788
SgrBI CCGCGG 1 cut(s) 449
SinI GGWCC 2 cut(s) 207, 238
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
SphI GCATGC 2 cut(s) 102, 481
Sse9I AATT 2 cut(s) 381, 391
SsiI CCGC 7 cut(s) 65, 68, 86, 190, 217, 446, 448
SstI GAGCTC 2 cut(s) 42, 228
StyD4I CCNGG 2 cut(s) 245, 613
StyI CCWWGG 1 cut(s) 296
TaaI ACNGT 2 cut(s) 311, 494
TaiI ACGT 4 cut(s) 95, 444, 528, 646
TaqI TCGA 4 cut(s) 174, 287, 389, 788
TasI AATT 2 cut(s) 381, 391
TatI WGTACW 1 cut(s) 306
TauI GCSGC 2 cut(s) 68, 71
TfiI GAWTC 1 cut(s) 271
Tru1I TTAA 2 cut(s) 44, 489
Tru9I TTAA 2 cut(s) 44, 489
TscAI CASTG 1 cut(s) 140
TseFI GTSAC 2 cut(s) 8, 781
TseI GCWGC 3 cut(s) 266, 346, 848
Tsp45I GTSAC 2 cut(s) 8, 781
TspDTI ATGAA 4 cut(s) 111, 536, 582, 595
TspRI CASTG 1 cut(s) 140
VpaK11BI GGWCC 2 cut(s) 207, 238
XapI RAATTY 2 cut(s) 381, 391
XceI RCATGY 3 cut(s) 102, 481, 676
XmiI GTMKAC 1 cut(s) 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.