FvH4_4g36890

Photosystem II stability assembly factor HCF136

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009356
Physical Location & Seq
Forward (+)
33623719 .. 33627555
3837 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g36890.t1

Sequence Viewer

Length: 1221 bp
ATGGCGAGGACTCTGCAACTCCTCTCTACTGATTTCTCCTCCAACCCAATGCTTCTCCTCAAACCCTCTCTCACCTCCCTCTTCGTCTCCACTCCTCCACGTCACTTCCCGACCACCTCTCTCCCTCGCTTCATTCCCCGCGCCTCCCTCACTCGCCGCCACCTCGTCTCCGACACGGCGGCGCTCTCTCTCGCCCTCACCGTGCCTTTGCTCGGCTCATCCGCTCTACCGGCCAAGTCCGAAGAGCCCGCCCTCTCCGAGTGGGAGAAAGTCTCCCTCCCTATCGACCCCGGCGTCGTCCTCCTCGACATCGCTTTCGTCCCCGACGACCCCAAACACGGTTTTCTATTGGGGACGAGGCAGACACTACTGGAGACCAAGGACGGCGGACAGAGCTGGGCGCCGCGGTCGATTCCGTCAGCCGAAGACGAAGATTTCAACTACAGATTTAATGCCATAAGCTTCAAAGGGAAGGAGGGATGGATTGTTGGAAAGCCTGCTATCCTGTTGCACACTTCTGATGCTGGCGACAGCTGGGAACGCATTCCGCTAAGCTCTCAGCTTCCCGGTGATATGGTGTATATAAAGGCAACTGGTGACAAGAGCGCCGAGATGGTGACTGACGAAGGTGCGATTTACGTGACCTCCAATAGGGGATACAACTGGAAGGCTGCTGTTCAGGAGACTGTTTCAGCTACTCTTAATAGAACAGTTTCTAGTGGCATTAGCGGTGCAAGTTACTACACGGGAACTTTTAACGCTGTCAATCGCTCGCCAGATGGAAGCTATGTTGCTGTCTCAAGTCGCGGTAACTTCTTCCTTACTTGGGAGCCTGGTCAGCCATACTGGCAGCCACATAACAGATCAGTTGCTAGAAGAATCCAAAATATGGGTTGGAGAGCTGATGGTGGTCTTTGGCTTCTTGTTCGTGGAGGAGGACTTTTTCTGAGCAAAGGTACAGGGATAACAGAGGAGTTTGAAGAAGTTCCAGTCCAAAGCCGGGGATTTGGCATTCTTGATGTTGGGTACCGATCAAAGGATGAGGCTTGGGCTGCAGGCGGAAGTGGGATTTTATTGCGAACTACCAATGGTGGCAAGAGCTGGGTCCGTGACAAAGCAGCCGATAATATTGCCGCAAATCTATACTCTGTAAAGTTTATTGATGACAATCAAGGATTTGTGCTGGGGAACGATGGGGTCTTGCTACGGTATCTTGGTTGA

Protein Analysis

407

Amino Acids

44.15

Weight (kDa)

6.55

Isoelectric Point (pI)

33.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSII_BNR PF14870 84 - 404 9.1e-125 Photosynthesis system II assembly factor YCF48
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 293
Acc65I GGTACC 1 cut(s) 1026
AccB1I GGYRCC 2 cut(s) 400, 1026
AccB7I CCANNNNNTGG 1 cut(s) 889
AccBSI CCGCTC 1 cut(s) 224
AccII CGCG 3 cut(s) 141, 406, 807
AcoI YGGCCR 1 cut(s) 231
AcyI GRCGYC 2 cut(s) 294, 401
AfaI GTAC 2 cut(s) 958, 1028
AfiI CCNNNNNNNGG 7 cut(s) 212, 338, 651, 826, 889, 1000, 1036
AgsI TTSAA 3 cut(s) 439, 466, 980
AjiI CACGTC 1 cut(s) 101
AjnI CCWGG 1 cut(s) 832
AluBI AGCT 9 cut(s) 396, 462, 534, 555, 562, 695, 786, 902, 1101
AluI AGCT 9 cut(s) 396, 462, 534, 555, 562, 695, 786, 902, 1101
Alw26I GTCTC 6 cut(s) 91, 172, 277, 368, 677, 802
AoxI GGCC 1 cut(s) 231
ApeKI GCWGC 4 cut(s) 671, 850, 1052, 1118
ArsI GACNNNNNNTTYG 2 cut(s) 299, 331
Asp700I GAANNNNTTC 3 cut(s) 543, 712, 984
Asp718I GGTACC 1 cut(s) 1026
AspLEI GCGC 4 cut(s) 143, 184, 403, 608
AspS9I GGNCC 1 cut(s) 1105
AsuC2I CCSGG 3 cut(s) 291, 567, 1001
AsuHPI GGTGA 5 cut(s) 64, 190, 581, 608, 628
AvaII GGWCC 1 cut(s) 1105
BanI GGYRCC 2 cut(s) 400, 1026
BanII GRGCYC 1 cut(s) 249
BbsI GAAGAC 1 cut(s) 432
BbvI GCAGC 4 cut(s) 658, 862, 1039, 1130
BccI CCATC 5 cut(s) 474, 607, 773, 899, 1187
BceAI ACGGC 2 cut(s) 192, 400
BcgI CGANNNNNNTGC 3 cut(s) 29, 1112, 1146
BciT130I CCWGG 1 cut(s) 834
BciVI GTATCC 1 cut(s) 650
BcnI CCSGG 3 cut(s) 291, 567, 1001
BcoDI GTCTC 6 cut(s) 91, 172, 277, 368, 677, 802
BfaI CTAG 2 cut(s) 717, 873
BfmI CTRYAG 2 cut(s) 442, 1053
BfoI RGCGCY 3 cut(s) 185, 404, 609
BfuI GTATCC 1 cut(s) 650
BglI GCCNNNNNGGC 1 cut(s) 847
BisI GCNGC 8 cut(s) 157, 180, 404, 672, 851, 1053, 1119, 1134
BlpI GCTNAGC 1 cut(s) 551
BlsI GCNGC 8 cut(s) 158, 181, 405, 673, 852, 1054, 1120, 1135
Bme1390I CCNGG 4 cut(s) 291, 567, 834, 1001
Bme18I GGWCC 1 cut(s) 1105
BmgBI CACGTC 1 cut(s) 101
BmgT120I GGNCC 1 cut(s) 1105
BmiI GGNNCC 4 cut(s) 402, 831, 1028, 1106
BmrFI CCNGG 4 cut(s) 291, 567, 834, 1001
BmsI GCATC 1 cut(s) 511
BpiI GAAGAC 1 cut(s) 432
BplI GAGNNNNNCTC 2 cut(s) 257, 289
BpmI CTGGAG 1 cut(s) 392
Bpu1102I GCTNAGC 1 cut(s) 551
BpuEI CTTGAG 1 cut(s) 784
BpuMI CCSGG 3 cut(s) 291, 567, 1001
BsaAI YACGTR 1 cut(s) 640
BsaBI GATNNNNATC 1 cut(s) 1167
BsaHI GRCGYC 2 cut(s) 294, 401
BsaI GGTCTC 1 cut(s) 368
BsaJI CCNNGG 4 cut(s) 289, 378, 404, 1000
BsaXI ACNNNNNCTCC 4 cut(s) 152, 182, 629, 659
Bsc4I CCNNNNNNNGG 7 cut(s) 212, 338, 651, 826, 889, 1000, 1036
Bse118I RCCGGY 1 cut(s) 229
Bse1I ACTGG 5 cut(s) 375, 598, 668, 851, 989
Bse8I GATNNNNATC 1 cut(s) 1167
BseBI CCWGG 1 cut(s) 834
BseDI CCNNGG 4 cut(s) 289, 378, 404, 1000
BseGI GGATG 3 cut(s) 218, 485, 1045
BseJI GATNNNNATC 1 cut(s) 1167
BseLI CCNNNNNNNGG 7 cut(s) 212, 338, 651, 826, 889, 1000, 1036
BseMII CTCAG 2 cut(s) 572, 938
BseNI ACTGG 5 cut(s) 375, 598, 668, 851, 989
BseRI GAGGAG 7 cut(s) 11, 28, 47, 84, 293, 948, 986
BseXI GCAGC 4 cut(s) 658, 862, 1039, 1130
BseYI CCCAGC 4 cut(s) 396, 534, 1101, 1183
Bsh1236I CGCG 3 cut(s) 141, 406, 807
Bsh1285I CGRYCG 1 cut(s) 410
BshFI GGCC 1 cut(s) 233
BshNI GGYRCC 2 cut(s) 400, 1026
BsiEI CGRYCG 1 cut(s) 410
BsiSI CCGG 4 cut(s) 230, 291, 567, 1000
BslFI GGGAC 2 cut(s) 305, 367
BslI CCNNNNNNNGG 7 cut(s) 212, 338, 651, 826, 889, 1000, 1036
BsmAI GTCTC 6 cut(s) 91, 172, 277, 368, 677, 802
BsmBI CGTCTC 2 cut(s) 91, 172
BsmFI GGGAC 2 cut(s) 305, 367
BsmI GAATGC 2 cut(s) 543, 1011
BsnI GGCC 1 cut(s) 233
Bso31I GGTCTC 1 cut(s) 368
Bsp1286I GDGCHC 1 cut(s) 249
Bsp143I GATC 2 cut(s) 863, 1031
Bsp1720I GCTNAGC 1 cut(s) 551
BspANI GGCC 1 cut(s) 233
BspCNI CTCAG 2 cut(s) 571, 939
BspFNI CGCG 3 cut(s) 141, 406, 807
BspLI GGNNCC 4 cut(s) 402, 831, 1028, 1106
BspMAI CTGCAG 1 cut(s) 1057
BspQI GCTCTTC 1 cut(s) 237
BspT107I GGYRCC 2 cut(s) 400, 1026
BspTNI GGTCTC 1 cut(s) 368
BsrBI CCGCTC 1 cut(s) 224
BsrFI RCCGGY 1 cut(s) 229
BsrI ACTGG 5 cut(s) 375, 598, 668, 851, 989
BssAI RCCGGY 1 cut(s) 229
BssECI CCNNGG 4 cut(s) 289, 378, 404, 1000
BssMI GATC 2 cut(s) 863, 1031
BssNI GRCGYC 2 cut(s) 294, 401
BssT1I CCWWGG 1 cut(s) 378
Bst2UI CCWGG 1 cut(s) 834
Bst4CI ACNGT 5 cut(s) 202, 341, 688, 712, 1209
Bst6I CTCTTC 2 cut(s) 86, 237
BstACI GRCGYC 2 cut(s) 294, 401
BstBAI YACGTR 1 cut(s) 640
BstC8I GCNNGC 5 cut(s) 249, 498, 526, 773, 1057
BstDEI CTNAG 3 cut(s) 551, 558, 947
BstDSI CCRYGG 1 cut(s) 404
BstENI CCTNNNNNAGG 1 cut(s) 649
BstF5I GGATG 3 cut(s) 218, 485, 1045
BstFNI CGCG 3 cut(s) 141, 406, 807
BstH2I RGCGCY 3 cut(s) 185, 404, 609
BstHHI GCGC 4 cut(s) 143, 184, 403, 608
BstKTI GATC 2 cut(s) 866, 1034
BstMAI GTCTC 6 cut(s) 91, 172, 277, 368, 677, 802
BstMBI GATC 2 cut(s) 863, 1031
BstMCI CGRYCG 1 cut(s) 410
BstMWI GCNNNNNNNGC 6 cut(s) 230, 393, 540, 838, 847, 1052
BstNI CCWGG 1 cut(s) 834
BstSCI CCNGG 4 cut(s) 289, 565, 832, 999
BstSFI CTRYAG 2 cut(s) 442, 1053
BstUI CGCG 3 cut(s) 141, 406, 807
BstV1I GCAGC 4 cut(s) 658, 862, 1039, 1130
BstV2I GAAGAC 1 cut(s) 432
BsuI GTATCC 1 cut(s) 650
BsuRI GGCC 1 cut(s) 233
BtgI CCRYGG 1 cut(s) 404
BtgZI GCGATG 1 cut(s) 295
BtrI CACGTC 1 cut(s) 101
BtsCI GGATG 3 cut(s) 218, 485, 1045
Cac8I GCNNGC 5 cut(s) 249, 498, 526, 773, 1057
CfoI GCGC 4 cut(s) 143, 184, 403, 608
Cfr10I RCCGGY 1 cut(s) 229
Cfr13I GGNCC 1 cut(s) 1105
Cfr42I CCGCGG 1 cut(s) 407
CseI GACGC 1 cut(s) 283
Csp6I GTAC 2 cut(s) 957, 1027
CviQI GTAC 2 cut(s) 957, 1027
DdeI CTNAG 3 cut(s) 551, 558, 947
DinI GGCGCC 1 cut(s) 402
DpnI GATC 2 cut(s) 865, 1033
DpnII GATC 2 cut(s) 863, 1031
DrdI GACNNNNNNGTC 1 cut(s) 293
DseDI GACNNNNNNGTC 1 cut(s) 293
EaeI YGGCCR 1 cut(s) 231
Eam1104I CTCTTC 2 cut(s) 86, 237
EarI CTCTTC 2 cut(s) 86, 237
EciI GGCGGA 2 cut(s) 402, 1074
Eco130I CCWWGG 1 cut(s) 378
Eco24I GRGCYC 1 cut(s) 249
Eco31I GGTCTC 1 cut(s) 368
Eco47I GGWCC 1 cut(s) 1105
EcoNI CCTNNNNNAGG 1 cut(s) 649
EcoRII CCWGG 1 cut(s) 832
EcoT14I CCWWGG 1 cut(s) 378
EcoT38I GRGCYC 1 cut(s) 249
EgeI GGCGCC 1 cut(s) 402
EheI GGCGCC 1 cut(s) 402
ErhI CCWWGG 1 cut(s) 378
Esp3I CGTCTC 2 cut(s) 91, 172
FaiI YATR 9 cut(s) 458, 575, 582, 584, 789, 844, 858, 890, 1144
FaqI GGGAC 2 cut(s) 305, 367
FauI CCCGC 2 cut(s) 146, 256
Fnu4HI GCNGC 8 cut(s) 157, 180, 404, 672, 851, 1053, 1119, 1134
FokI GGATG 3 cut(s) 205, 492, 1052
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 8 cut(s) 157, 180, 404, 672, 851, 1053, 1119, 1134
FspBI CTAG 2 cut(s) 717, 873
GlaI GCGC 4 cut(s) 142, 183, 402, 607
GluI GCNGC 8 cut(s) 157, 180, 404, 672, 851, 1053, 1119, 1134
GsaI CCCAGC 4 cut(s) 400, 538, 1105, 1187
GsuI CTGGAG 1 cut(s) 392
HaeII RGCGCY 3 cut(s) 185, 404, 609
HaeIII GGCC 1 cut(s) 233
HapII CCGG 4 cut(s) 230, 291, 567, 1000
HgaI GACGC 1 cut(s) 283
HhaI GCGC 4 cut(s) 143, 184, 403, 608
Hin1I GRCGYC 2 cut(s) 294, 401
Hin6I GCGC 4 cut(s) 141, 182, 401, 606
HinP1I GCGC 4 cut(s) 141, 182, 401, 606
HindIII AAGCTT 1 cut(s) 460
HinfI GANTC 3 cut(s) 10, 412, 879
HpaII CCGG 4 cut(s) 230, 291, 567, 1000
HphI GGTGA 5 cut(s) 64, 190, 581, 608, 628
Hpy188I TCNGA 5 cut(s) 172, 241, 259, 520, 948
Hpy188III TCNNGA 3 cut(s) 109, 680, 1016
Hpy99I CGWCG 2 cut(s) 299, 329
HpyAV CCTTC 3 cut(s) 466, 620, 661
HpyCH4III ACNGT 5 cut(s) 202, 341, 688, 712, 1209
HpyCH4IV ACGT 2 cut(s) 100, 639
HpyCH4V TGCA 4 cut(s) 16, 511, 734, 1055
HpyF10VI GCNNNNNNNGC 6 cut(s) 230, 393, 540, 838, 847, 1052
HpyF3I CTNAG 3 cut(s) 551, 558, 947
HpySE526I ACGT 2 cut(s) 100, 639
Hsp92I GRCGYC 2 cut(s) 294, 401
HspAI GCGC 4 cut(s) 141, 182, 401, 606
KasI GGCGCC 1 cut(s) 400
KpnI GGTACC 1 cut(s) 1030
KspI CCGCGG 1 cut(s) 407
Kzo9I GATC 2 cut(s) 863, 1031
LguI GCTCTTC 1 cut(s) 237
LmnI GCTCC 1 cut(s) 829
Lsp1109I GCAGC 4 cut(s) 658, 862, 1039, 1130
LweI GCATC 1 cut(s) 511
MaeI CTAG 2 cut(s) 717, 873
MaeII ACGT 2 cut(s) 100, 639
MaeIII GTNAC 7 cut(s) 101, 596, 616, 640, 737, 809, 1109
MalI GATC 2 cut(s) 865, 1033
MbiI CCGCTC 1 cut(s) 224
MboI GATC 2 cut(s) 863, 1031
MboII GAAGA 7 cut(s) 73, 254, 437, 443, 808, 888, 992
MhlI GDGCHC 1 cut(s) 249
Mly113I GGCGCC 1 cut(s) 401
MlyI GAGTC 1 cut(s) 4
MmeI TCCRAC 4 cut(s) 66, 195, 469, 875
MroXI GAANNNNTTC 3 cut(s) 543, 712, 984
MseI TTAA 3 cut(s) 450, 702, 756
MspA1I CMGCKG 2 cut(s) 406, 534
MspI CCGG 4 cut(s) 230, 291, 567, 1000
MspR9I CCNGG 4 cut(s) 291, 567, 834, 1001
Mva1269I GAATGC 2 cut(s) 543, 1011
MvaI CCWGG 1 cut(s) 834
MvnI CGCG 3 cut(s) 141, 406, 807
MwoI GCNNNNNNNGC 6 cut(s) 230, 393, 540, 838, 847, 1052
NarI GGCGCC 1 cut(s) 401
NciI CCSGG 3 cut(s) 291, 567, 1001
NdeII GATC 2 cut(s) 863, 1031
NlaIV GGNNCC 4 cut(s) 402, 831, 1028, 1106
NmeAIII GCCGAG 2 cut(s) 192, 634
NmuCI GTSAC 5 cut(s) 101, 596, 616, 640, 1109
PciSI GCTCTTC 1 cut(s) 237
PcsI WCGNNNNNNNCGW 4 cut(s) 198, 291, 303, 324
PctI GAATGC 2 cut(s) 543, 1011
PdmI GAANNNNTTC 3 cut(s) 543, 712, 984
PfeI GAWTC 2 cut(s) 412, 879
PflMI CCANNNNNTGG 1 cut(s) 889
PkrI GCNGC 8 cut(s) 158, 181, 405, 673, 852, 1054, 1120, 1135
PleI GAGTC 1 cut(s) 4
PluTI GGCGCC 1 cut(s) 404
PpsI GAGTC 1 cut(s) 4
Ppu21I YACGTR 1 cut(s) 640
Psp6I CCWGG 1 cut(s) 832
PspFI CCCAGC 4 cut(s) 396, 534, 1101, 1183
PspGI CCWGG 1 cut(s) 832
PspN4I GGNNCC 4 cut(s) 402, 831, 1028, 1106
PspPI GGNCC 1 cut(s) 1105
PstI CTGCAG 1 cut(s) 1057
PvuII CAGCTG 1 cut(s) 534
RsaI GTAC 2 cut(s) 958, 1028
RsaNI GTAC 2 cut(s) 957, 1027
SacII CCGCGG 1 cut(s) 407
SapI GCTCTTC 1 cut(s) 237
SaqAI TTAA 3 cut(s) 450, 702, 756
SatI GCNGC 8 cut(s) 157, 180, 404, 672, 851, 1053, 1119, 1134
Sau3AI GATC 2 cut(s) 863, 1031
Sau96I GGNCC 1 cut(s) 1105
SchI GAGTC 1 cut(s) 4
ScrFI CCNGG 4 cut(s) 291, 567, 834, 1001
SduI GDGCHC 1 cut(s) 249
SfaNI GCATC 1 cut(s) 511
SfcI CTRYAG 2 cut(s) 442, 1053
SfoI GGCGCC 1 cut(s) 402
Sfr303I CCGCGG 1 cut(s) 407
SgrBI CCGCGG 1 cut(s) 407
SinI GGWCC 1 cut(s) 1105
SmlI CTYRAG 1 cut(s) 799
SmoI CTYRAG 1 cut(s) 799
SspDI GGCGCC 1 cut(s) 400
SspI AATATT 1 cut(s) 1129
SspMI CTAG 2 cut(s) 717, 873
StyD4I CCNGG 4 cut(s) 289, 565, 832, 999
StyI CCWWGG 1 cut(s) 378
TaaI ACNGT 5 cut(s) 202, 341, 688, 712, 1209
TaiI ACGT 2 cut(s) 103, 642
TaqI TCGA 3 cut(s) 285, 306, 410
TauI GCSGC 4 cut(s) 159, 182, 406, 1136
TfiI GAWTC 2 cut(s) 412, 879
Tru1I TTAA 3 cut(s) 450, 702, 756
Tru9I TTAA 3 cut(s) 450, 702, 756
TseFI GTSAC 5 cut(s) 101, 596, 616, 640, 1109
TseI GCWGC 4 cut(s) 671, 850, 1052, 1118
Tsp45I GTSAC 5 cut(s) 101, 596, 616, 640, 1109
TspDTI ATGAA 1 cut(s) 121
TspGWI ACGGA 2 cut(s) 405, 1097
Van91I CCANNNNNTGG 1 cut(s) 889
VpaK11BI GGWCC 1 cut(s) 1105
XagI CCTNNNNNAGG 1 cut(s) 649
XmnI GAANNNNTTC 3 cut(s) 543, 712, 984
XspI CTAG 2 cut(s) 717, 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.