RLG00000005641

Photosystem II stability assembly factor HCF136

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0009356
Physical Location & Seq
Reverse (-)
523964 .. 527318
3355 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005641

Sequence Viewer

Length: 1221 bp
ATGGCGACTCTGCAACTCCTCACTACAGATTTCTCCAAACCGATGATTCTCCTCAAACCCTCCCTCTTCGTCTCCAGTCCTCCACGTCATTGCCCCACCTCTCTCCCTCGCTTCATTCCCAGAGCCTCTTCTTCTTCTTCTTCTTCTCCGCTCAGTCGCCGCCACTTCGTCTCCGAGACGGCGGCGCTGTCTCTCGCCCTAACCGTGCCGTTTCTCGGTTCCCCTCAACCGGCCAAGTCCGAAGAGCCCGCCCTCTCCGAGTGGGAGAAAGTCTCCCTCCCTATCGACTCCGGCGTCGTCCTTCTCGACATCGCTTTCGTCCCCGACGACCCTAAACACGGATTTCTGTTGGGAACCAGGCAGACACTTTTGGAGACCAAGGACGGTGGACAAAGCTGGGCGCCGCGGTCAATTCCATCTGCTGAAGATGAAGATTTTAACTACAGATTCAATGCCATAAGCTTCAAAGGGAACGAGGGCTGGATTGTCGGAAAGCCTGCAATTCTGTTACACACTTCTGATGCTGGCGACAGCTGGGAACGGATACCGCTAAGCTCTCAGCTTCCGGGAGATATGGTATATATAAAGGCAACTGGTGAGAAGAGCGCAGAGATGGTGACTGACGAAGGTGCGATTTATGTGACCTCCAATAGGGGATACAATTGGAGGGCTGCTGTTCAGGAGACTGTTTCAGCTACTCTTAATAGAACAGTCTCTAGTGGCATTAGCGGTGCAAGTTACTACACAGGAACTTTTAATGCGGTCAATCGCTCGCCAAATGGAAGCTATGTTGCTGTCTCAAGTCGTGGTAACTTCTTCCTTACTTGGGAGCCTGGTCAGCCATACTGGCAGCCACATAACAGATCAGTTGCAAGAAGAATCCAAAACATGGGTTGGAGAGCTGATGGTGGTCTTTGGCTTCTTGTTCGAGGAGGGGGACTTTTTCTGAGCAAAGGTACAGGGATAACAGAGGAGTTTGAAGAAGTTCCAGTCCAAAGCCGGGGTTTTGGCATTCTTGATGTTGGGTACCGATCAAAGGATGAGGCTTGGGCAGCAGGTGGAAGTGGGATTTTATTGCGAACTACCAATGGTGGCAAGACATGGGTCCGTGACAAAGCAGCCGACAATATTGCCGCAAATCTATACTCTGTAAAGTTTATTGATGATAATCAAGGATTTGTGCTGGGGAACGATGGGGTATTGCTTCGGTATCTTGGATGA

Protein Analysis

407

Amino Acids

44.15

Weight (kDa)

6.55

Isoelectric Point (pI)

41.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSII_BNR PF14870 84 - 404 6e-125 Photosynthesis system II assembly factor YCF48
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1046
AasI GACNNNNNNGTC 1 cut(s) 293
Acc36I ACCTGC 1 cut(s) 1046
Acc65I GGTACC 1 cut(s) 1026
AccB1I GGYRCC 2 cut(s) 400, 1026
AccB7I CCANNNNNTGG 1 cut(s) 889
AccBSI CCGCTC 1 cut(s) 151
AccII CGCG 1 cut(s) 406
AcoI YGGCCR 1 cut(s) 231
AcuI CTGAAG 1 cut(s) 444
AcyI GRCGYC 2 cut(s) 294, 401
AfaI GTAC 2 cut(s) 958, 1028
AfiI CCNNNNNNNGG 8 cut(s) 215, 229, 338, 651, 826, 889, 1000, 1036
AgsI TTSAA 3 cut(s) 451, 466, 980
AjiI CACGTC 1 cut(s) 86
AjnI CCWGG 2 cut(s) 356, 832
AluBI AGCT 8 cut(s) 396, 462, 534, 555, 562, 695, 786, 902
AluI AGCT 8 cut(s) 396, 462, 534, 555, 562, 695, 786, 902
Alw26I GTCTC 9 cut(s) 76, 170, 175, 195, 277, 368, 677, 718, 802
AoxI GGCC 1 cut(s) 231
ApeKI GCWGC 4 cut(s) 671, 850, 1052, 1118
ArsI GACNNNNNNTTYG 2 cut(s) 299, 331
Asp700I GAANNNNTTC 1 cut(s) 984
Asp718I GGTACC 1 cut(s) 1026
AspLEI GCGC 3 cut(s) 187, 403, 608
AspS9I GGNCC 1 cut(s) 1105
AsuC2I CCSGG 2 cut(s) 567, 1001
AsuHPI GGTGA 2 cut(s) 608, 628
AvaII GGWCC 1 cut(s) 1105
BanI GGYRCC 2 cut(s) 400, 1026
BanII GRGCYC 1 cut(s) 249
BbvI GCAGC 4 cut(s) 658, 862, 1064, 1130
BccI CCATC 4 cut(s) 424, 607, 899, 1187
BceAI ACGGC 2 cut(s) 193, 195
BcgI CGANNNNNNTGC 2 cut(s) 1112, 1146
BciT130I CCWGG 2 cut(s) 358, 834
BciVI GTATCC 2 cut(s) 537, 650
BcnI CCSGG 2 cut(s) 567, 1001
BcoDI GTCTC 9 cut(s) 76, 170, 175, 195, 277, 368, 677, 718, 802
BfaI CTAG 1 cut(s) 717
BfmI CTRYAG 2 cut(s) 24, 442
BfoI RGCGCY 2 cut(s) 188, 404
BfuAI ACCTGC 1 cut(s) 1046
BfuI GTATCC 2 cut(s) 537, 650
BglI GCCNNNNNGGC 1 cut(s) 847
BisI GCNGC 8 cut(s) 160, 183, 404, 672, 851, 1053, 1119, 1134
BlpI GCTNAGC 1 cut(s) 551
BlsI GCNGC 8 cut(s) 161, 184, 405, 673, 852, 1054, 1120, 1135
Bme1390I CCNGG 4 cut(s) 358, 567, 834, 1001
Bme18I GGWCC 1 cut(s) 1105
BmgBI CACGTC 1 cut(s) 86
BmgT120I GGNCC 1 cut(s) 1105
BmiI GGNNCC 6 cut(s) 220, 355, 402, 831, 1028, 1106
BmrFI CCNGG 4 cut(s) 358, 567, 834, 1001
BmsI GCATC 1 cut(s) 511
BoxI GACNNNNGTC 1 cut(s) 1103
BplI GAGNNNNNCTC 2 cut(s) 257, 289
BpmI CTGGAG 1 cut(s) 58
Bpu1102I GCTNAGC 1 cut(s) 551
BpuEI CTTGAG 1 cut(s) 784
BpuMI CCSGG 2 cut(s) 567, 1001
BsaBI GATNNNNATC 1 cut(s) 1167
BsaHI GRCGYC 2 cut(s) 294, 401
BsaI GGTCTC 1 cut(s) 368
BsaJI CCNNGG 3 cut(s) 378, 404, 1000
BsaXI ACNNNNNCTCC 4 cut(s) 155, 185, 561, 591
Bsc4I CCNNNNNNNGG 8 cut(s) 215, 229, 338, 651, 826, 889, 1000, 1036
Bse118I RCCGGY 1 cut(s) 229
Bse1I ACTGG 4 cut(s) 75, 598, 851, 989
Bse3DI GCAATG 1 cut(s) 88
Bse8I GATNNNNATC 1 cut(s) 1167
BseBI CCWGG 2 cut(s) 358, 834
BseDI CCNNGG 3 cut(s) 378, 404, 1000
BseGI GGATG 1 cut(s) 1045
BseJI GATNNNNATC 1 cut(s) 1167
BseLI CCNNNNNNNGG 8 cut(s) 215, 229, 338, 651, 826, 889, 1000, 1036
BseMI GCAATG 1 cut(s) 88
BseMII CTCAG 3 cut(s) 166, 572, 938
BseNI ACTGG 4 cut(s) 75, 598, 851, 989
BseRI GAGGAG 4 cut(s) 8, 41, 945, 986
BseXI GCAGC 4 cut(s) 658, 862, 1064, 1130
BseYI CCCAGC 3 cut(s) 396, 534, 1183
Bsh1236I CGCG 1 cut(s) 406
BshFI GGCC 1 cut(s) 233
BshNI GGYRCC 2 cut(s) 400, 1026
BsiSI CCGG 4 cut(s) 230, 291, 566, 1000
BslFI GGGAC 2 cut(s) 305, 951
BslI CCNNNNNNNGG 8 cut(s) 215, 229, 338, 651, 826, 889, 1000, 1036
BsmAI GTCTC 9 cut(s) 76, 170, 175, 195, 277, 368, 677, 718, 802
BsmBI CGTCTC 3 cut(s) 76, 170, 175
BsmFI GGGAC 2 cut(s) 305, 951
BsmI GAATGC 1 cut(s) 1011
BsnI GGCC 1 cut(s) 233
Bso31I GGTCTC 1 cut(s) 368
Bsp1286I GDGCHC 1 cut(s) 249
Bsp143I GATC 2 cut(s) 863, 1031
Bsp1720I GCTNAGC 1 cut(s) 551
BspANI GGCC 1 cut(s) 233
BspCNI CTCAG 3 cut(s) 165, 571, 939
BspFNI CGCG 1 cut(s) 406
BspLI GGNNCC 6 cut(s) 220, 355, 402, 831, 1028, 1106
BspMI ACCTGC 1 cut(s) 1046
BspQI GCTCTTC 2 cut(s) 237, 596
BspT107I GGYRCC 2 cut(s) 400, 1026
BspTNI GGTCTC 1 cut(s) 368
BsrBI CCGCTC 1 cut(s) 151
BsrDI GCAATG 1 cut(s) 88
BsrFI RCCGGY 1 cut(s) 229
BsrI ACTGG 4 cut(s) 75, 598, 851, 989
BssAI RCCGGY 1 cut(s) 229
BssECI CCNNGG 3 cut(s) 378, 404, 1000
BssMI GATC 2 cut(s) 863, 1031
BssNI GRCGYC 2 cut(s) 294, 401
BssT1I CCWWGG 1 cut(s) 378
Bst2UI CCWGG 2 cut(s) 358, 834
Bst4CI ACNGT 4 cut(s) 205, 386, 688, 712
Bst6I CTCTTC 4 cut(s) 71, 133, 237, 596
BstACI GRCGYC 2 cut(s) 294, 401
BstC8I GCNNGC 4 cut(s) 249, 498, 526, 773
BstDEI CTNAG 4 cut(s) 152, 551, 558, 947
BstDSI CCRYGG 1 cut(s) 404
BstENI CCTNNNNNAGG 1 cut(s) 649
BstF5I GGATG 1 cut(s) 1045
BstFNI CGCG 1 cut(s) 406
BstH2I RGCGCY 2 cut(s) 188, 404
BstHHI GCGC 3 cut(s) 187, 403, 608
BstKTI GATC 2 cut(s) 866, 1034
BstMAI GTCTC 9 cut(s) 76, 170, 175, 195, 277, 368, 677, 718, 802
BstMBI GATC 2 cut(s) 863, 1031
BstMWI GCNNNNNNNGC 3 cut(s) 838, 847, 1052
BstNI CCWGG 2 cut(s) 358, 834
BstPAI GACNNNNGTC 1 cut(s) 1103
BstSCI CCNGG 4 cut(s) 356, 565, 832, 999
BstSFI CTRYAG 2 cut(s) 24, 442
BstUI CGCG 1 cut(s) 406
BstV1I GCAGC 4 cut(s) 658, 862, 1064, 1130
BsuI GTATCC 2 cut(s) 537, 650
BsuRI GGCC 1 cut(s) 233
BtgI CCRYGG 1 cut(s) 404
BtgZI GCGATG 1 cut(s) 295
BtrI CACGTC 1 cut(s) 86
BtsCI GGATG 1 cut(s) 1045
BveI ACCTGC 1 cut(s) 1046
Cac8I GCNNGC 4 cut(s) 249, 498, 526, 773
CfoI GCGC 3 cut(s) 187, 403, 608
Cfr10I RCCGGY 1 cut(s) 229
Cfr13I GGNCC 1 cut(s) 1105
Cfr42I CCGCGG 1 cut(s) 407
CseI GACGC 1 cut(s) 283
Csp6I GTAC 2 cut(s) 957, 1027
CspCI CAANNNNNGTGG 2 cut(s) 367, 402
CviAII CATG 2 cut(s) 889, 1101
CviQI GTAC 2 cut(s) 957, 1027
DdeI CTNAG 4 cut(s) 152, 551, 558, 947
DinI GGCGCC 1 cut(s) 402
DpnI GATC 2 cut(s) 865, 1033
DpnII GATC 2 cut(s) 863, 1031
DrdI GACNNNNNNGTC 1 cut(s) 293
DseDI GACNNNNNNGTC 1 cut(s) 293
EaeI YGGCCR 1 cut(s) 231
Eam1104I CTCTTC 4 cut(s) 71, 133, 237, 596
EarI CTCTTC 4 cut(s) 71, 133, 237, 596
Eco130I CCWWGG 1 cut(s) 378
Eco24I GRGCYC 1 cut(s) 249
Eco31I GGTCTC 1 cut(s) 368
Eco47I GGWCC 1 cut(s) 1105
Eco57I CTGAAG 1 cut(s) 444
EcoNI CCTNNNNNAGG 1 cut(s) 649
EcoRII CCWGG 2 cut(s) 356, 832
EcoT14I CCWWGG 1 cut(s) 378
EcoT38I GRGCYC 1 cut(s) 249
EgeI GGCGCC 1 cut(s) 402
EheI GGCGCC 1 cut(s) 402
ErhI CCWWGG 1 cut(s) 378
Esp3I CGTCTC 3 cut(s) 76, 170, 175
FaeI CATG 2 cut(s) 892, 1104
FaqI GGGAC 2 cut(s) 305, 951
FatI CATG 2 cut(s) 888, 1100
FauI CCCGC 1 cut(s) 256
Fnu4HI GCNGC 8 cut(s) 160, 183, 404, 672, 851, 1053, 1119, 1134
FokI GGATG 1 cut(s) 1052
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 8 cut(s) 160, 183, 404, 672, 851, 1053, 1119, 1134
FspBI CTAG 1 cut(s) 717
GlaI GCGC 3 cut(s) 186, 402, 607
GluI GCNGC 8 cut(s) 160, 183, 404, 672, 851, 1053, 1119, 1134
GsaI CCCAGC 3 cut(s) 400, 538, 1187
GsuI CTGGAG 1 cut(s) 58
HaeII RGCGCY 2 cut(s) 188, 404
HaeIII GGCC 1 cut(s) 233
HapII CCGG 4 cut(s) 230, 291, 566, 1000
HgaI GACGC 1 cut(s) 283
HhaI GCGC 3 cut(s) 187, 403, 608
Hin1I GRCGYC 2 cut(s) 294, 401
Hin1II CATG 2 cut(s) 892, 1104
Hin6I GCGC 3 cut(s) 185, 401, 606
HinP1I GCGC 3 cut(s) 185, 401, 606
HindIII AAGCTT 1 cut(s) 460
HinfI GANTC 5 cut(s) 7, 46, 287, 447, 879
HpaII CCGG 4 cut(s) 230, 291, 566, 1000
HphI GGTGA 2 cut(s) 608, 628
Hpy166II GTNNAC 1 cut(s) 389
Hpy188I TCNGA 6 cut(s) 175, 241, 259, 491, 520, 948
Hpy188III TCNNGA 3 cut(s) 305, 680, 1016
Hpy8I GTNNAC 1 cut(s) 389
Hpy99I CGWCG 2 cut(s) 299, 329
HpyAV CCTTC 2 cut(s) 311, 620
HpyCH4III ACNGT 4 cut(s) 205, 386, 688, 712
HpyCH4IV ACGT 1 cut(s) 85
HpyCH4V TGCA 4 cut(s) 13, 500, 734, 872
HpyF10VI GCNNNNNNNGC 3 cut(s) 838, 847, 1052
HpyF3I CTNAG 4 cut(s) 152, 551, 558, 947
HpySE526I ACGT 1 cut(s) 85
Hsp92I GRCGYC 2 cut(s) 294, 401
Hsp92II CATG 2 cut(s) 892, 1104
HspAI GCGC 3 cut(s) 185, 401, 606
KasI GGCGCC 1 cut(s) 400
KpnI GGTACC 1 cut(s) 1030
KspI CCGCGG 1 cut(s) 407
Kzo9I GATC 2 cut(s) 863, 1031
LguI GCTCTTC 2 cut(s) 237, 596
LmnI GCTCC 1 cut(s) 829
Lsp1109I GCAGC 4 cut(s) 658, 862, 1064, 1130
LweI GCATC 1 cut(s) 511
MaeI CTAG 1 cut(s) 717
MaeII ACGT 1 cut(s) 85
MaeIII GTNAC 6 cut(s) 507, 616, 640, 737, 809, 1109
MalI GATC 2 cut(s) 865, 1033
MbiI CCGCTC 1 cut(s) 151
MboI GATC 2 cut(s) 863, 1031
MfeI CAATTG 1 cut(s) 661
MhlI GDGCHC 1 cut(s) 249
MluCI AATT 3 cut(s) 411, 501, 661
Mly113I GGCGCC 1 cut(s) 401
MlyI GAGTC 1 cut(s) 281
MmeI TCCRAC 2 cut(s) 469, 875
MroXI GAANNNNTTC 1 cut(s) 984
MseI TTAA 3 cut(s) 438, 702, 756
MspA1I CMGCKG 2 cut(s) 406, 534
MspI CCGG 4 cut(s) 230, 291, 566, 1000
MspR9I CCNGG 4 cut(s) 358, 567, 834, 1001
MunI CAATTG 1 cut(s) 661
Mva1269I GAATGC 1 cut(s) 1011
MvaI CCWGG 2 cut(s) 358, 834
MvnI CGCG 1 cut(s) 406
MwoI GCNNNNNNNGC 3 cut(s) 838, 847, 1052
NarI GGCGCC 1 cut(s) 401
NciI CCSGG 2 cut(s) 567, 1001
NdeII GATC 2 cut(s) 863, 1031
NlaIII CATG 2 cut(s) 892, 1104
NlaIV GGNNCC 6 cut(s) 220, 355, 402, 831, 1028, 1106
NmuCI GTSAC 3 cut(s) 616, 640, 1109
PaqCI CACCTGC 1 cut(s) 1046
PciSI GCTCTTC 2 cut(s) 237, 596
PcsI WCGNNNNNNNCGW 4 cut(s) 201, 291, 303, 324
PctI GAATGC 1 cut(s) 1011
PdmI GAANNNNTTC 1 cut(s) 984
PfeI GAWTC 3 cut(s) 46, 447, 879
PflMI CCANNNNNTGG 1 cut(s) 889
PfoI TCCNGGA 1 cut(s) 565
PkrI GCNGC 8 cut(s) 161, 184, 405, 673, 852, 1054, 1120, 1135
PleI GAGTC 1 cut(s) 281
PluTI GGCGCC 1 cut(s) 404
PpsI GAGTC 1 cut(s) 281
PshAI GACNNNNGTC 1 cut(s) 1103
Psp6I CCWGG 2 cut(s) 356, 832
PspFI CCCAGC 3 cut(s) 396, 534, 1183
PspGI CCWGG 2 cut(s) 356, 832
PspN4I GGNNCC 6 cut(s) 220, 355, 402, 831, 1028, 1106
PspPI GGNCC 1 cut(s) 1105
PvuII CAGCTG 1 cut(s) 534
RsaI GTAC 2 cut(s) 958, 1028
RsaNI GTAC 2 cut(s) 957, 1027
SacII CCGCGG 1 cut(s) 407
SapI GCTCTTC 2 cut(s) 237, 596
SaqAI TTAA 3 cut(s) 438, 702, 756
SatI GCNGC 8 cut(s) 160, 183, 404, 672, 851, 1053, 1119, 1134
Sau3AI GATC 2 cut(s) 863, 1031
Sau96I GGNCC 1 cut(s) 1105
SchI GAGTC 1 cut(s) 281
ScrFI CCNGG 4 cut(s) 358, 567, 834, 1001
SduI GDGCHC 1 cut(s) 249
SfaNI GCATC 1 cut(s) 511
SfcI CTRYAG 2 cut(s) 24, 442
SfoI GGCGCC 1 cut(s) 402
Sfr303I CCGCGG 1 cut(s) 407
SgrBI CCGCGG 1 cut(s) 407
SinI GGWCC 1 cut(s) 1105
SmlI CTYRAG 1 cut(s) 799
SmoI CTYRAG 1 cut(s) 799
Sse9I AATT 3 cut(s) 411, 501, 661
SspDI GGCGCC 1 cut(s) 400
SspI AATATT 1 cut(s) 1129
SspMI CTAG 1 cut(s) 717
StyD4I CCNGG 4 cut(s) 356, 565, 832, 999
StyI CCWWGG 1 cut(s) 378
TaaI ACNGT 4 cut(s) 205, 386, 688, 712
TaiI ACGT 1 cut(s) 88
TaqI TCGA 3 cut(s) 285, 306, 928
TasI AATT 3 cut(s) 411, 501, 661
TauI GCSGC 4 cut(s) 162, 185, 406, 1136
TfiI GAWTC 3 cut(s) 46, 447, 879
Tru1I TTAA 3 cut(s) 438, 702, 756
Tru9I TTAA 3 cut(s) 438, 702, 756
TseFI GTSAC 3 cut(s) 616, 640, 1109
TseI GCWGC 4 cut(s) 671, 850, 1052, 1118
Tsp45I GTSAC 3 cut(s) 616, 640, 1109
TspDTI ATGAA 2 cut(s) 103, 444
TspGWI ACGGA 3 cut(s) 354, 556, 1097
Van91I CCANNNNNTGG 1 cut(s) 889
VpaK11BI GGWCC 1 cut(s) 1105
XagI CCTNNNNNAGG 1 cut(s) 649
XmnI GAANNNNTTC 1 cut(s) 984
XspI CTAG 1 cut(s) 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.