Rh4CG461300

Photosystem II stability assembly factor HCF136

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0009356
Physical Location & Seq
Forward (+)
69253718 .. 69257389
3672 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG461300.1

Sequence Viewer

Length: 1224 bp
ATGGCGACTCTGCAGCTCCTCACTACAGATTTCTCCAAACCAATGATTCTCCTCAAACCCTCTCTCTTCGTCTCCAGTACTCCTCCTCCACGTCATTGCCCCACCTCTCTCCCTCGCTTCATTCCCAGAGCCTCTTCTTCTTCTTCTTCTCCCCTCAGTCGCCGCCACTTCGTCTCCGAGACGGCGGCGCTGTCTCTCGCCCTAACCGTGCCGTTGCTCGGCTCCCCTCAACCGGCCAAGTCCGAAGAGCCCGCCCTCTCCGAGTGGGAGAAAGTCTCCCTCCCTATCGACCCCGGCGTCGTCCTTCTCGACATCGCTTTCGTCCCCGACGACCCTAAACACGGATTTCTGTTGGGAACAAGGCAGACACTTTTGGAGACCAAGGACGGTGGACAAAGCTGGGCGCCGCGGTCAATTCCGTCTGCTGAAGATGAAGATTTTAACTACAGATTCAATGCCATAAGCTTCAAAGGGAAGGAGGGCTGGATTGTCGGAAAGCCTGCAATTCTGTTACACACTTCTGATGCTGGCGACAGCTGGGAACGGATACCGCTAAGCTCTCAGCTTCCAGGAGATATGGTATATATAAAGGCAACTGGTGAGAAGAGCGCAGAGATGGTGACTGACGAAGGTGCGATTTATGTGACCTCCAATAGGGGATACAATTGGAGGGCTGCTGTTCAGGAGACTGTTTCAGCTACTCTTAATAGAACAGTCTCTAGTGGCATTAGCGGTGCAAGTTACTACACAGGAACTTTTAATGCGGTCAATCGCTCGCCAAATGGAAGCTATGTTGCTGTCTCAAGTCGTGGTAACTTCTTCCTTACTTGGGAGCCTGGTCAGCCATACTGGCAGCCACATAACAGATCAATTGCAAGAAGAATCCAAAACATGGGTTGGAGAGCTGATGGTGGTCTTTGGCTTCTTGTTCGTGGAGGGGGACTTTTTCTGAGCAAAGGTACAGGGATAACAGAGGAGTTTGAAGAAGTTCCAGTCCAAAGCCGGGGTTTTGGCATTCTTGATGTTGGGTACCGATCAAAGGATGAGGCTTGGGCAGCAGGTGGAAGTGGGATTTTATTGCGAACTACCAATGGTGGCAAGACATGGGTCCGTGACAAAGCAGCCGACAATATTGCTGCAAATCTATACTCTGTAAAGTTTATCGATGACAATCAAGGATTTGTGCTGGGGAACGATGGGGTATTGCTTCGGTATCTTGGATGA

Protein Analysis

407

Amino Acids

44.26

Weight (kDa)

6.99

Isoelectric Point (pI)

39.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSII_BNR PF14870 85 - 405 2e-124 Photosynthesis system II assembly factor YCF48
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1049
AasI GACNNNNNNGTC 1 cut(s) 296
Acc36I ACCTGC 1 cut(s) 1049
Acc65I GGTACC 1 cut(s) 1029
AccB1I GGYRCC 2 cut(s) 403, 1029
AccB7I CCANNNNNTGG 1 cut(s) 892
AccII CGCG 1 cut(s) 409
AciI CCGC 8 cut(s) 163, 185, 252, 407, 409, 551, 732, 764
AcoI YGGCCR 1 cut(s) 234
AcuI CTGAAG 1 cut(s) 447
AcyI GRCGYC 2 cut(s) 297, 404
AfaI GTAC 3 cut(s) 79, 961, 1031
AfiI CCNNNNNNNGG 8 cut(s) 218, 232, 341, 654, 829, 892, 1003, 1039
AgsI TTSAA 3 cut(s) 454, 469, 983
AjiI CACGTC 1 cut(s) 92
AjnI CCWGG 2 cut(s) 568, 835
AluBI AGCT 9 cut(s) 16, 399, 465, 537, 558, 565, 698, 789, 905
AluI AGCT 9 cut(s) 16, 399, 465, 537, 558, 565, 698, 789, 905
Alw26I GTCTC 9 cut(s) 76, 173, 178, 198, 280, 371, 680, 721, 805
AoxI GGCC 1 cut(s) 234
ApeKI GCWGC 6 cut(s) 13, 674, 853, 1055, 1121, 1136
ArsI GACNNNNNNTTYG 2 cut(s) 302, 334
Asp700I GAANNNNTTC 1 cut(s) 987
Asp718I GGTACC 1 cut(s) 1029
AspLEI GCGC 3 cut(s) 190, 406, 611
AspS9I GGNCC 1 cut(s) 1108
AsuC2I CCSGG 2 cut(s) 294, 1004
AsuHPI GGTGA 2 cut(s) 611, 631
AvaII GGWCC 1 cut(s) 1108
BanI GGYRCC 2 cut(s) 403, 1029
BanII GRGCYC 1 cut(s) 252
BbvI GCAGC 6 cut(s) 25, 661, 865, 1067, 1123, 1133
BccI CCATC 3 cut(s) 610, 902, 1190
BceAI ACGGC 2 cut(s) 196, 198
BcgI CGANNNNNNTGC 2 cut(s) 1115, 1149
BciT130I CCWGG 2 cut(s) 570, 837
BciVI GTATCC 2 cut(s) 540, 653
BcnI CCSGG 2 cut(s) 294, 1004
BcoDI GTCTC 9 cut(s) 76, 173, 178, 198, 280, 371, 680, 721, 805
BfaI CTAG 1 cut(s) 720
BfmI CTRYAG 3 cut(s) 11, 24, 445
BfoI RGCGCY 2 cut(s) 191, 407
BfuAI ACCTGC 1 cut(s) 1049
BfuI GTATCC 2 cut(s) 540, 653
BglI GCCNNNNNGGC 1 cut(s) 850
BisI GCNGC 9 cut(s) 14, 163, 186, 407, 675, 854, 1056, 1122, 1137
BlpI GCTNAGC 1 cut(s) 554
BlsI GCNGC 9 cut(s) 15, 164, 187, 408, 676, 855, 1057, 1123, 1138
BmcAI AGTACT 1 cut(s) 79
Bme1390I CCNGG 4 cut(s) 294, 570, 837, 1004
Bme18I GGWCC 1 cut(s) 1108
BmgBI CACGTC 1 cut(s) 92
BmgT120I GGNCC 1 cut(s) 1108
BmiI GGNNCC 5 cut(s) 223, 405, 834, 1031, 1109
BmrFI CCNGG 4 cut(s) 294, 570, 837, 1004
BmsI GCATC 1 cut(s) 514
BoxI GACNNNNGTC 1 cut(s) 1106
BplI GAGNNNNNCTC 2 cut(s) 260, 292
BpmI CTGGAG 1 cut(s) 58
Bpu1102I GCTNAGC 1 cut(s) 554
BpuEI CTTGAG 1 cut(s) 787
BpuMI CCSGG 2 cut(s) 294, 1004
Bsa29I ATCGAT 1 cut(s) 1164
BsaBI GATNNNNATC 1 cut(s) 1170
BsaHI GRCGYC 2 cut(s) 297, 404
BsaI GGTCTC 1 cut(s) 371
BsaJI CCNNGG 4 cut(s) 292, 381, 407, 1003
BsaXI ACNNNNNCTCC 6 cut(s) 70, 100, 158, 188, 564, 594
Bsc4I CCNNNNNNNGG 8 cut(s) 218, 232, 341, 654, 829, 892, 1003, 1039
Bse118I RCCGGY 1 cut(s) 232
Bse1I ACTGG 4 cut(s) 75, 601, 854, 992
Bse3DI GCAATG 1 cut(s) 94
Bse8I GATNNNNATC 1 cut(s) 1170
BseBI CCWGG 2 cut(s) 570, 837
BseCI ATCGAT 1 cut(s) 1164
BseDI CCNNGG 4 cut(s) 292, 381, 407, 1003
BseGI GGATG 1 cut(s) 1048
BseJI GATNNNNATC 1 cut(s) 1170
BseLI CCNNNNNNNGG 8 cut(s) 218, 232, 341, 654, 829, 892, 1003, 1039
BseMI GCAATG 1 cut(s) 94
BseMII CTCAG 3 cut(s) 169, 575, 941
BseNI ACTGG 4 cut(s) 75, 601, 854, 992
BseRI GAGGAG 5 cut(s) 8, 41, 72, 75, 989
BseXI GCAGC 6 cut(s) 25, 661, 865, 1067, 1123, 1133
BseYI CCCAGC 3 cut(s) 399, 537, 1186
Bsh1236I CGCG 1 cut(s) 409
BshFI GGCC 1 cut(s) 236
BshNI GGYRCC 2 cut(s) 403, 1029
BshVI ATCGAT 1 cut(s) 1164
BsiSI CCGG 3 cut(s) 233, 294, 1003
BslFI GGGAC 2 cut(s) 308, 954
BslI CCNNNNNNNGG 8 cut(s) 218, 232, 341, 654, 829, 892, 1003, 1039
BsmAI GTCTC 9 cut(s) 76, 173, 178, 198, 280, 371, 680, 721, 805
BsmBI CGTCTC 3 cut(s) 76, 173, 178
BsmFI GGGAC 2 cut(s) 308, 954
BsmI GAATGC 1 cut(s) 1014
BsnI GGCC 1 cut(s) 236
Bso31I GGTCTC 1 cut(s) 371
Bsp1286I GDGCHC 1 cut(s) 252
Bsp143I GATC 2 cut(s) 866, 1034
Bsp1720I GCTNAGC 1 cut(s) 554
BspACI CCGC 8 cut(s) 163, 185, 252, 407, 409, 551, 732, 764
BspANI GGCC 1 cut(s) 236
BspCNI CTCAG 3 cut(s) 168, 574, 942
BspDI ATCGAT 1 cut(s) 1164
BspFNI CGCG 1 cut(s) 409
BspLI GGNNCC 5 cut(s) 223, 405, 834, 1031, 1109
BspMAI CTGCAG 1 cut(s) 15
BspMI ACCTGC 1 cut(s) 1049
BspQI GCTCTTC 2 cut(s) 240, 599
BspT107I GGYRCC 2 cut(s) 403, 1029
BspTNI GGTCTC 1 cut(s) 371
BsrDI GCAATG 1 cut(s) 94
BsrFI RCCGGY 1 cut(s) 232
BsrI ACTGG 4 cut(s) 75, 601, 854, 992
BssAI RCCGGY 1 cut(s) 232
BssECI CCNNGG 4 cut(s) 292, 381, 407, 1003
BssMI GATC 2 cut(s) 866, 1034
BssNI GRCGYC 2 cut(s) 297, 404
BssT1I CCWWGG 1 cut(s) 381
Bst2UI CCWGG 2 cut(s) 570, 837
Bst4CI ACNGT 4 cut(s) 208, 389, 691, 715
Bst6I CTCTTC 4 cut(s) 71, 139, 240, 599
BstACI GRCGYC 2 cut(s) 297, 404
BstC8I GCNNGC 4 cut(s) 252, 501, 529, 776
BstDEI CTNAG 4 cut(s) 155, 554, 561, 950
BstDSI CCRYGG 1 cut(s) 407
BstENI CCTNNNNNAGG 1 cut(s) 652
BstF5I GGATG 1 cut(s) 1048
BstFNI CGCG 1 cut(s) 409
BstH2I RGCGCY 2 cut(s) 191, 407
BstHHI GCGC 3 cut(s) 190, 406, 611
BstKTI GATC 2 cut(s) 869, 1037
BstMAI GTCTC 9 cut(s) 76, 173, 178, 198, 280, 371, 680, 721, 805
BstMBI GATC 2 cut(s) 866, 1034
BstMWI GCNNNNNNNGC 3 cut(s) 841, 850, 1055
BstNI CCWGG 2 cut(s) 570, 837
BstPAI GACNNNNGTC 1 cut(s) 1106
BstSCI CCNGG 4 cut(s) 292, 568, 835, 1002
BstSFI CTRYAG 3 cut(s) 11, 24, 445
BstUI CGCG 1 cut(s) 409
BstV1I GCAGC 6 cut(s) 25, 661, 865, 1067, 1123, 1133
Bsu15I ATCGAT 1 cut(s) 1164
BsuI GTATCC 2 cut(s) 540, 653
BsuRI GGCC 1 cut(s) 236
BsuTUI ATCGAT 1 cut(s) 1164
BtgI CCRYGG 1 cut(s) 407
BtgZI GCGATG 1 cut(s) 298
BtrI CACGTC 1 cut(s) 92
BtsCI GGATG 1 cut(s) 1048
BveI ACCTGC 1 cut(s) 1049
Cac8I GCNNGC 4 cut(s) 252, 501, 529, 776
CfoI GCGC 3 cut(s) 190, 406, 611
Cfr10I RCCGGY 1 cut(s) 232
Cfr13I GGNCC 1 cut(s) 1108
Cfr42I CCGCGG 1 cut(s) 410
ClaI ATCGAT 1 cut(s) 1164
CseI GACGC 1 cut(s) 286
Csp6I GTAC 3 cut(s) 78, 960, 1030
CspCI CAANNNNNGTGG 2 cut(s) 370, 405
CviAII CATG 2 cut(s) 892, 1104
CviQI GTAC 3 cut(s) 78, 960, 1030
DdeI CTNAG 4 cut(s) 155, 554, 561, 950
DinI GGCGCC 1 cut(s) 405
DpnI GATC 2 cut(s) 868, 1036
DpnII GATC 2 cut(s) 866, 1034
DrdI GACNNNNNNGTC 1 cut(s) 296
DseDI GACNNNNNNGTC 1 cut(s) 296
EaeI YGGCCR 1 cut(s) 234
Eam1104I CTCTTC 4 cut(s) 71, 139, 240, 599
EarI CTCTTC 4 cut(s) 71, 139, 240, 599
Eco130I CCWWGG 1 cut(s) 381
Eco24I GRGCYC 1 cut(s) 252
Eco31I GGTCTC 1 cut(s) 371
Eco47I GGWCC 1 cut(s) 1108
Eco57I CTGAAG 1 cut(s) 447
EcoNI CCTNNNNNAGG 1 cut(s) 652
EcoRII CCWGG 2 cut(s) 568, 835
EcoT14I CCWWGG 1 cut(s) 381
EcoT38I GRGCYC 1 cut(s) 252
EgeI GGCGCC 1 cut(s) 405
EheI GGCGCC 1 cut(s) 405
ErhI CCWWGG 1 cut(s) 381
Esp3I CGTCTC 3 cut(s) 76, 173, 178
FaeI CATG 2 cut(s) 895, 1107
FaqI GGGAC 2 cut(s) 308, 954
FatI CATG 2 cut(s) 891, 1103
FauI CCCGC 1 cut(s) 259
Fnu4HI GCNGC 9 cut(s) 14, 163, 186, 407, 675, 854, 1056, 1122, 1137
FokI GGATG 1 cut(s) 1055
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 9 cut(s) 14, 163, 186, 407, 675, 854, 1056, 1122, 1137
FspBI CTAG 1 cut(s) 720
GlaI GCGC 3 cut(s) 189, 405, 610
GluI GCNGC 9 cut(s) 14, 163, 186, 407, 675, 854, 1056, 1122, 1137
GsaI CCCAGC 3 cut(s) 403, 541, 1190
GsuI CTGGAG 1 cut(s) 58
HaeII RGCGCY 2 cut(s) 191, 407
HaeIII GGCC 1 cut(s) 236
HapII CCGG 3 cut(s) 233, 294, 1003
HgaI GACGC 1 cut(s) 286
HhaI GCGC 3 cut(s) 190, 406, 611
Hin1I GRCGYC 2 cut(s) 297, 404
Hin1II CATG 2 cut(s) 895, 1107
Hin6I GCGC 3 cut(s) 188, 404, 609
HinP1I GCGC 3 cut(s) 188, 404, 609
HindIII AAGCTT 1 cut(s) 463
HinfI GANTC 4 cut(s) 7, 46, 450, 882
HpaII CCGG 3 cut(s) 233, 294, 1003
HphI GGTGA 2 cut(s) 611, 631
Hpy166II GTNNAC 1 cut(s) 392
Hpy188I TCNGA 6 cut(s) 178, 244, 262, 494, 523, 951
Hpy188III TCNNGA 3 cut(s) 308, 683, 1019
Hpy8I GTNNAC 1 cut(s) 392
Hpy99I CGWCG 2 cut(s) 302, 332
HpyAV CCTTC 3 cut(s) 314, 469, 623
HpyCH4III ACNGT 4 cut(s) 208, 389, 691, 715
HpyCH4IV ACGT 1 cut(s) 91
HpyCH4V TGCA 5 cut(s) 13, 503, 737, 875, 1139
HpyF10VI GCNNNNNNNGC 3 cut(s) 841, 850, 1055
HpyF3I CTNAG 4 cut(s) 155, 554, 561, 950
HpySE526I ACGT 1 cut(s) 91
Hsp92I GRCGYC 2 cut(s) 297, 404
Hsp92II CATG 2 cut(s) 895, 1107
HspAI GCGC 3 cut(s) 188, 404, 609
KasI GGCGCC 1 cut(s) 403
KpnI GGTACC 1 cut(s) 1033
KspI CCGCGG 1 cut(s) 410
Kzo9I GATC 2 cut(s) 866, 1034
LguI GCTCTTC 2 cut(s) 240, 599
LmnI GCTCC 3 cut(s) 21, 227, 832
Lsp1109I GCAGC 6 cut(s) 25, 661, 865, 1067, 1123, 1133
LweI GCATC 1 cut(s) 514
MaeI CTAG 1 cut(s) 720
MaeII ACGT 1 cut(s) 91
MaeIII GTNAC 6 cut(s) 510, 619, 643, 740, 812, 1112
MalI GATC 2 cut(s) 868, 1036
MboI GATC 2 cut(s) 866, 1034
MfeI CAATTG 2 cut(s) 664, 870
MhlI GDGCHC 1 cut(s) 252
MluCI AATT 4 cut(s) 414, 504, 664, 870
Mly113I GGCGCC 1 cut(s) 404
MmeI TCCRAC 2 cut(s) 472, 878
MroXI GAANNNNTTC 1 cut(s) 987
MseI TTAA 3 cut(s) 441, 705, 759
MspA1I CMGCKG 2 cut(s) 409, 537
MspI CCGG 3 cut(s) 233, 294, 1003
MspR9I CCNGG 4 cut(s) 294, 570, 837, 1004
MunI CAATTG 2 cut(s) 664, 870
Mva1269I GAATGC 1 cut(s) 1014
MvaI CCWGG 2 cut(s) 570, 837
MvnI CGCG 1 cut(s) 409
MwoI GCNNNNNNNGC 3 cut(s) 841, 850, 1055
NarI GGCGCC 1 cut(s) 404
NciI CCSGG 2 cut(s) 294, 1004
NdeII GATC 2 cut(s) 866, 1034
NlaIII CATG 2 cut(s) 895, 1107
NlaIV GGNNCC 5 cut(s) 223, 405, 834, 1031, 1109
NmeAIII GCCGAG 1 cut(s) 198
NmuCI GTSAC 3 cut(s) 619, 643, 1112
PaqCI CACCTGC 1 cut(s) 1049
PciSI GCTCTTC 2 cut(s) 240, 599
PcsI WCGNNNNNNNCGW 4 cut(s) 204, 294, 306, 327
PctI GAATGC 1 cut(s) 1014
PdmI GAANNNNTTC 1 cut(s) 987
PfeI GAWTC 3 cut(s) 46, 450, 882
PflMI CCANNNNNTGG 1 cut(s) 892
PfoI TCCNGGA 1 cut(s) 568
PkrI GCNGC 9 cut(s) 15, 164, 187, 408, 676, 855, 1057, 1123, 1138
PluTI GGCGCC 1 cut(s) 407
PshAI GACNNNNGTC 1 cut(s) 1106
Psp6I CCWGG 2 cut(s) 568, 835
PspFI CCCAGC 3 cut(s) 399, 537, 1186
PspGI CCWGG 2 cut(s) 568, 835
PspN4I GGNNCC 5 cut(s) 223, 405, 834, 1031, 1109
PspPI GGNCC 1 cut(s) 1108
PstI CTGCAG 1 cut(s) 15
PvuII CAGCTG 1 cut(s) 537
RsaI GTAC 3 cut(s) 79, 961, 1031
RsaNI GTAC 3 cut(s) 78, 960, 1030
SacII CCGCGG 1 cut(s) 410
SapI GCTCTTC 2 cut(s) 240, 599
SaqAI TTAA 3 cut(s) 441, 705, 759
SatI GCNGC 9 cut(s) 14, 163, 186, 407, 675, 854, 1056, 1122, 1137
Sau3AI GATC 2 cut(s) 866, 1034
Sau96I GGNCC 1 cut(s) 1108
ScaI AGTACT 1 cut(s) 79
ScrFI CCNGG 4 cut(s) 294, 570, 837, 1004
SduI GDGCHC 1 cut(s) 252
SfaNI GCATC 1 cut(s) 514
SfcI CTRYAG 3 cut(s) 11, 24, 445
SfoI GGCGCC 1 cut(s) 405
Sfr303I CCGCGG 1 cut(s) 410
SgrBI CCGCGG 1 cut(s) 410
SinI GGWCC 1 cut(s) 1108
SmlI CTYRAG 1 cut(s) 802
SmoI CTYRAG 1 cut(s) 802
Sse9I AATT 4 cut(s) 414, 504, 664, 870
SsiI CCGC 8 cut(s) 163, 185, 252, 407, 409, 551, 732, 764
SspDI GGCGCC 1 cut(s) 403
SspI AATATT 1 cut(s) 1132
SspMI CTAG 1 cut(s) 720
StyD4I CCNGG 4 cut(s) 292, 568, 835, 1002
StyI CCWWGG 1 cut(s) 381
TaaI ACNGT 4 cut(s) 208, 389, 691, 715
TaiI ACGT 1 cut(s) 94
TaqI TCGA 3 cut(s) 288, 309, 1164
TasI AATT 4 cut(s) 414, 504, 664, 870
TatI WGTACW 1 cut(s) 77
TauI GCSGC 3 cut(s) 165, 188, 409
TfiI GAWTC 3 cut(s) 46, 450, 882
Tru1I TTAA 3 cut(s) 441, 705, 759
Tru9I TTAA 3 cut(s) 441, 705, 759
TseFI GTSAC 3 cut(s) 619, 643, 1112
TseI GCWGC 6 cut(s) 13, 674, 853, 1055, 1121, 1136
Tsp45I GTSAC 3 cut(s) 619, 643, 1112
TspDTI ATGAA 2 cut(s) 109, 447
TspGWI ACGGA 4 cut(s) 357, 408, 559, 1100
Van91I CCANNNNNTGG 1 cut(s) 892
VpaK11BI GGWCC 1 cut(s) 1108
XagI CCTNNNNNAGG 1 cut(s) 652
XmnI GAANNNNTTC 1 cut(s) 987
XspI CTAG 1 cut(s) 720
ZrmI AGTACT 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.