RchiOBHm_Chr4g0446671

Photosystem II stability assembly factor HCF136

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0009356
Physical Location & Seq
Forward (+)
66765712 .. 66769702
3991 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41417

Sequence Viewer

Length: 1212 bp
ATGGCGACTCTGCAACTCCTCACTACAGATTTCTCCAAACCAATGATTCTCCTCAAACCCTCTCTCTTCGTCTCCACTCCTCCACGTCATTGCCCCACCTCTCTCCCTCGCTTCATTCCCAGAGCCTCTTCTTCTTCTCCGCTCAGTCGCCGCCACTTCGTCTCCGAGACGGCGGCGCTGTCTCTCGCCCTAACCGTGCCGTTGCTGGGTTCCCCTCAACTGGCCAAGTCCGAAGAGCCCGCCCTCTCCGAGTGGGAGAAAGTCTCCCTCCCTATCGACCCCGGCGTCGTCCTTCTCGACATCGCTTTCGTCCCCGACGACCCTAAACACGGATTTCTGTTGGGAACAAGGCAGACACTTTTGGAGACCAAGGACGGTGGACAAAGCTGGGCGCCGCGGTCAATTCCGTCTGCTGAAGATGAAGATTTTAACTACAGGTTCAATGCCATAAGCTTCAAAGGGAAGGAGGGCTGGATTGTCGGAAAGCCTGCAATTCTGTTGCACACTTCTGATGCTGGCGACAGCTGGGAACGGATACCGCTAAGCTCTCAGCTTCCGGGAGATATGGTATATATAAAGGCAACTGGTGAGAAGAGCGCAGAGATGGTGACTGACGAAGGTGCGATTTATGTGACCTCCAATAGGGGATACAATTGGAGGGCTGCTGTTCAGGAGACTGTTTCTGCTACTCTTAATAGAACAGTCTCTAGTGGCATTAGCGGTGCAAGTTACTACACAGGAACTTTTAATGCCGTCAATCGCTCGCCAAATGGAAGCTATGTTGCTGTCTCAAGTCGTGGTAACTTCTTCCTTACTTGGGAGCCTGGTCAGCCATACTGGCAGCCACATAACAGATCAGTTGCAAGAAGAATCCAAAACATGGGTTGGAGAGCTGATGGTGGTCTTTGGCTTCTTGTTCGTGGAGGGGGACTTTTTCTGAGCAAAGGTACAGGGATAACAGAGGAGTTTGAAGAAGTTCCAGTCCAAAGCCGGGGTTTTGGCATTCTTGATGTTGGGTACCGATCAAAGGATGAGGCTTGGGCAGCAGGTGGAAGTGGGATTTTATTGCGAACTACCAATGGTGGCAAGACATGGGTCCGTGACAAAGCAGCCGACAATATTGCTGCAAATCTATACTCTGTAAAGTTTATCGATGACAATCAAGGATTTGTGCTGGGGAACGATGGGGTATTGCTTCGGTATCTTGGATGA

Protein Analysis

403

Amino Acids

43.9

Weight (kDa)

6.99

Isoelectric Point (pI)

36.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSII_BNR PF14870 81 - 401 1.3e-124 Photosynthesis system II assembly factor YCF48
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1037
AasI GACNNNNNNGTC 1 cut(s) 284
Acc36I ACCTGC 1 cut(s) 1037
Acc65I GGTACC 1 cut(s) 1017
AccB1I GGYRCC 2 cut(s) 391, 1017
AccB7I CCANNNNNTGG 1 cut(s) 880
AccBSI CCGCTC 1 cut(s) 142
AccII CGCG 1 cut(s) 397
AciI CCGC 8 cut(s) 140, 151, 173, 240, 395, 397, 539, 720
AcoI YGGCCR 1 cut(s) 222
AcuI CTGAAG 1 cut(s) 435
AcyI GRCGYC 2 cut(s) 285, 392
AfaI GTAC 2 cut(s) 949, 1019
AfiI CCNNNNNNNGG 8 cut(s) 206, 220, 329, 642, 817, 880, 991, 1027
AgsI TTSAA 3 cut(s) 442, 457, 971
AjiI CACGTC 1 cut(s) 86
AjnI CCWGG 1 cut(s) 823
AluBI AGCT 7 cut(s) 387, 453, 525, 546, 553, 777, 893
AluI AGCT 7 cut(s) 387, 453, 525, 546, 553, 777, 893
Alw26I GTCTC 9 cut(s) 76, 161, 166, 186, 268, 359, 668, 709, 793
AoxI GGCC 1 cut(s) 222
ApeKI GCWGC 5 cut(s) 662, 841, 1043, 1109, 1124
ArsI GACNNNNNNTTYG 2 cut(s) 290, 322
Asp700I GAANNNNTTC 1 cut(s) 975
Asp718I GGTACC 1 cut(s) 1017
AspLEI GCGC 3 cut(s) 178, 394, 599
AspS9I GGNCC 1 cut(s) 1096
AsuC2I CCSGG 3 cut(s) 282, 558, 992
AsuHPI GGTGA 2 cut(s) 599, 619
AvaII GGWCC 1 cut(s) 1096
BalI TGGCCA 1 cut(s) 224
BanI GGYRCC 2 cut(s) 391, 1017
BanII GRGCYC 1 cut(s) 240
BbvI GCAGC 5 cut(s) 649, 853, 1055, 1111, 1121
BccI CCATC 3 cut(s) 598, 890, 1178
BceAI ACGGC 3 cut(s) 184, 186, 737
BcgI CGANNNNNNTGC 2 cut(s) 1103, 1137
BciT130I CCWGG 1 cut(s) 825
BciVI GTATCC 2 cut(s) 528, 641
BcnI CCSGG 3 cut(s) 282, 558, 992
BcoDI GTCTC 9 cut(s) 76, 161, 166, 186, 268, 359, 668, 709, 793
BfaI CTAG 1 cut(s) 708
BfmI CTRYAG 2 cut(s) 24, 433
BfoI RGCGCY 2 cut(s) 179, 395
BfuAI ACCTGC 1 cut(s) 1037
BfuI GTATCC 2 cut(s) 528, 641
BglI GCCNNNNNGGC 1 cut(s) 838
BisI GCNGC 8 cut(s) 151, 174, 395, 663, 842, 1044, 1110, 1125
BlpI GCTNAGC 1 cut(s) 542
BlsI GCNGC 8 cut(s) 152, 175, 396, 664, 843, 1045, 1111, 1126
Bme1390I CCNGG 4 cut(s) 282, 558, 825, 992
Bme18I GGWCC 1 cut(s) 1096
BmgBI CACGTC 1 cut(s) 86
BmgT120I GGNCC 1 cut(s) 1096
BmiI GGNNCC 5 cut(s) 211, 393, 822, 1019, 1097
BmrFI CCNGG 4 cut(s) 282, 558, 825, 992
BmsI GCATC 1 cut(s) 502
BoxI GACNNNNGTC 1 cut(s) 1094
BplI GAGNNNNNCTC 2 cut(s) 248, 280
Bpu1102I GCTNAGC 1 cut(s) 542
BpuEI CTTGAG 1 cut(s) 775
BpuMI CCSGG 3 cut(s) 282, 558, 992
Bsa29I ATCGAT 1 cut(s) 1152
BsaBI GATNNNNATC 1 cut(s) 1158
BsaHI GRCGYC 2 cut(s) 285, 392
BsaI GGTCTC 1 cut(s) 359
BsaJI CCNNGG 4 cut(s) 280, 369, 395, 991
BsaXI ACNNNNNCTCC 4 cut(s) 146, 176, 552, 582
Bsc4I CCNNNNNNNGG 8 cut(s) 206, 220, 329, 642, 817, 880, 991, 1027
Bse1I ACTGG 4 cut(s) 225, 589, 842, 980
Bse3DI GCAATG 1 cut(s) 88
Bse8I GATNNNNATC 1 cut(s) 1158
BseBI CCWGG 1 cut(s) 825
BseCI ATCGAT 1 cut(s) 1152
BseDI CCNNGG 4 cut(s) 280, 369, 395, 991
BseGI GGATG 1 cut(s) 1036
BseJI GATNNNNATC 1 cut(s) 1158
BseLI CCNNNNNNNGG 8 cut(s) 206, 220, 329, 642, 817, 880, 991, 1027
BseMI GCAATG 1 cut(s) 88
BseMII CTCAG 3 cut(s) 157, 563, 929
BseNI ACTGG 4 cut(s) 225, 589, 842, 980
BseRI GAGGAG 4 cut(s) 8, 41, 69, 977
BseXI GCAGC 5 cut(s) 649, 853, 1055, 1111, 1121
BseYI CCCAGC 4 cut(s) 205, 387, 525, 1174
Bsh1236I CGCG 1 cut(s) 397
BshFI GGCC 1 cut(s) 224
BshNI GGYRCC 2 cut(s) 391, 1017
BshVI ATCGAT 1 cut(s) 1152
BsiSI CCGG 3 cut(s) 282, 557, 991
BslFI GGGAC 2 cut(s) 296, 942
BslI CCNNNNNNNGG 8 cut(s) 206, 220, 329, 642, 817, 880, 991, 1027
BsmAI GTCTC 9 cut(s) 76, 161, 166, 186, 268, 359, 668, 709, 793
BsmBI CGTCTC 3 cut(s) 76, 161, 166
BsmFI GGGAC 2 cut(s) 296, 942
BsmI GAATGC 1 cut(s) 1002
BsnI GGCC 1 cut(s) 224
Bso31I GGTCTC 1 cut(s) 359
Bsp1286I GDGCHC 1 cut(s) 240
Bsp143I GATC 2 cut(s) 854, 1022
Bsp1720I GCTNAGC 1 cut(s) 542
BspACI CCGC 8 cut(s) 140, 151, 173, 240, 395, 397, 539, 720
BspANI GGCC 1 cut(s) 224
BspCNI CTCAG 3 cut(s) 156, 562, 930
BspDI ATCGAT 1 cut(s) 1152
BspFNI CGCG 1 cut(s) 397
BspLI GGNNCC 5 cut(s) 211, 393, 822, 1019, 1097
BspMI ACCTGC 1 cut(s) 1037
BspQI GCTCTTC 2 cut(s) 228, 587
BspT107I GGYRCC 2 cut(s) 391, 1017
BspTNI GGTCTC 1 cut(s) 359
BsrBI CCGCTC 1 cut(s) 142
BsrDI GCAATG 1 cut(s) 88
BsrI ACTGG 4 cut(s) 225, 589, 842, 980
BssECI CCNNGG 4 cut(s) 280, 369, 395, 991
BssMI GATC 2 cut(s) 854, 1022
BssNI GRCGYC 2 cut(s) 285, 392
BssT1I CCWWGG 1 cut(s) 369
Bst2UI CCWGG 1 cut(s) 825
Bst4CI ACNGT 4 cut(s) 196, 377, 679, 703
Bst6I CTCTTC 4 cut(s) 71, 133, 228, 587
BstACI GRCGYC 2 cut(s) 285, 392
BstC8I GCNNGC 4 cut(s) 240, 489, 517, 764
BstDEI CTNAG 4 cut(s) 143, 542, 549, 938
BstDSI CCRYGG 1 cut(s) 395
BstENI CCTNNNNNAGG 1 cut(s) 640
BstF5I GGATG 1 cut(s) 1036
BstFNI CGCG 1 cut(s) 397
BstH2I RGCGCY 2 cut(s) 179, 395
BstHHI GCGC 3 cut(s) 178, 394, 599
BstKTI GATC 2 cut(s) 857, 1025
BstMAI GTCTC 9 cut(s) 76, 161, 166, 186, 268, 359, 668, 709, 793
BstMBI GATC 2 cut(s) 854, 1022
BstMWI GCNNNNNNNGC 3 cut(s) 829, 838, 1043
BstNI CCWGG 1 cut(s) 825
BstPAI GACNNNNGTC 1 cut(s) 1094
BstSCI CCNGG 4 cut(s) 280, 556, 823, 990
BstSFI CTRYAG 2 cut(s) 24, 433
BstUI CGCG 1 cut(s) 397
BstV1I GCAGC 5 cut(s) 649, 853, 1055, 1111, 1121
Bsu15I ATCGAT 1 cut(s) 1152
BsuI GTATCC 2 cut(s) 528, 641
BsuRI GGCC 1 cut(s) 224
BsuTUI ATCGAT 1 cut(s) 1152
BtgI CCRYGG 1 cut(s) 395
BtgZI GCGATG 1 cut(s) 286
BtrI CACGTC 1 cut(s) 86
BtsCI GGATG 1 cut(s) 1036
BveI ACCTGC 1 cut(s) 1037
Cac8I GCNNGC 4 cut(s) 240, 489, 517, 764
CfoI GCGC 3 cut(s) 178, 394, 599
Cfr13I GGNCC 1 cut(s) 1096
Cfr42I CCGCGG 1 cut(s) 398
ClaI ATCGAT 1 cut(s) 1152
CseI GACGC 1 cut(s) 274
Csp6I GTAC 2 cut(s) 948, 1018
CspCI CAANNNNNGTGG 2 cut(s) 358, 393
CviAII CATG 2 cut(s) 880, 1092
CviQI GTAC 2 cut(s) 948, 1018
DdeI CTNAG 4 cut(s) 143, 542, 549, 938
DinI GGCGCC 1 cut(s) 393
DpnI GATC 2 cut(s) 856, 1024
DpnII GATC 2 cut(s) 854, 1022
DrdI GACNNNNNNGTC 1 cut(s) 284
DseDI GACNNNNNNGTC 1 cut(s) 284
EaeI YGGCCR 1 cut(s) 222
Eam1104I CTCTTC 4 cut(s) 71, 133, 228, 587
EarI CTCTTC 4 cut(s) 71, 133, 228, 587
Eco130I CCWWGG 1 cut(s) 369
Eco24I GRGCYC 1 cut(s) 240
Eco31I GGTCTC 1 cut(s) 359
Eco47I GGWCC 1 cut(s) 1096
Eco57I CTGAAG 1 cut(s) 435
EcoNI CCTNNNNNAGG 1 cut(s) 640
EcoRII CCWGG 1 cut(s) 823
EcoT14I CCWWGG 1 cut(s) 369
EcoT38I GRGCYC 1 cut(s) 240
EgeI GGCGCC 1 cut(s) 393
EheI GGCGCC 1 cut(s) 393
ErhI CCWWGG 1 cut(s) 369
Esp3I CGTCTC 3 cut(s) 76, 161, 166
FaeI CATG 2 cut(s) 883, 1095
FaqI GGGAC 2 cut(s) 296, 942
FatI CATG 2 cut(s) 879, 1091
FauI CCCGC 1 cut(s) 247
Fnu4HI GCNGC 8 cut(s) 151, 174, 395, 663, 842, 1044, 1110, 1125
FokI GGATG 1 cut(s) 1043
FriOI GRGCYC 1 cut(s) 240
Fsp4HI GCNGC 8 cut(s) 151, 174, 395, 663, 842, 1044, 1110, 1125
FspBI CTAG 1 cut(s) 708
GlaI GCGC 3 cut(s) 177, 393, 598
GluI GCNGC 8 cut(s) 151, 174, 395, 663, 842, 1044, 1110, 1125
GsaI CCCAGC 4 cut(s) 209, 391, 529, 1178
HaeII RGCGCY 2 cut(s) 179, 395
HaeIII GGCC 1 cut(s) 224
HapII CCGG 3 cut(s) 282, 557, 991
HgaI GACGC 1 cut(s) 274
HhaI GCGC 3 cut(s) 178, 394, 599
Hin1I GRCGYC 2 cut(s) 285, 392
Hin1II CATG 2 cut(s) 883, 1095
Hin6I GCGC 3 cut(s) 176, 392, 597
HinP1I GCGC 3 cut(s) 176, 392, 597
HindIII AAGCTT 1 cut(s) 451
HinfI GANTC 3 cut(s) 7, 46, 870
HpaII CCGG 3 cut(s) 282, 557, 991
HphI GGTGA 2 cut(s) 599, 619
Hpy166II GTNNAC 1 cut(s) 380
Hpy188I TCNGA 6 cut(s) 166, 232, 250, 482, 511, 939
Hpy188III TCNNGA 3 cut(s) 296, 671, 1007
Hpy8I GTNNAC 1 cut(s) 380
Hpy99I CGWCG 2 cut(s) 290, 320
HpyAV CCTTC 3 cut(s) 302, 457, 611
HpyCH4III ACNGT 4 cut(s) 196, 377, 679, 703
HpyCH4IV ACGT 1 cut(s) 85
HpyCH4V TGCA 6 cut(s) 13, 491, 502, 725, 863, 1127
HpyF10VI GCNNNNNNNGC 3 cut(s) 829, 838, 1043
HpyF3I CTNAG 4 cut(s) 143, 542, 549, 938
HpySE526I ACGT 1 cut(s) 85
Hsp92I GRCGYC 2 cut(s) 285, 392
Hsp92II CATG 2 cut(s) 883, 1095
HspAI GCGC 3 cut(s) 176, 392, 597
KasI GGCGCC 1 cut(s) 391
KpnI GGTACC 1 cut(s) 1021
KspI CCGCGG 1 cut(s) 398
Kzo9I GATC 2 cut(s) 854, 1022
LguI GCTCTTC 2 cut(s) 228, 587
LmnI GCTCC 1 cut(s) 820
Lsp1109I GCAGC 5 cut(s) 649, 853, 1055, 1111, 1121
LweI GCATC 1 cut(s) 502
MaeI CTAG 1 cut(s) 708
MaeII ACGT 1 cut(s) 85
MaeIII GTNAC 5 cut(s) 607, 631, 728, 800, 1100
MalI GATC 2 cut(s) 856, 1024
MbiI CCGCTC 1 cut(s) 142
MboI GATC 2 cut(s) 854, 1022
MfeI CAATTG 1 cut(s) 652
MhlI GDGCHC 1 cut(s) 240
MlsI TGGCCA 1 cut(s) 224
MluCI AATT 3 cut(s) 402, 492, 652
MluNI TGGCCA 1 cut(s) 224
Mly113I GGCGCC 1 cut(s) 392
MmeI TCCRAC 2 cut(s) 460, 866
Mox20I TGGCCA 1 cut(s) 224
MroXI GAANNNNTTC 1 cut(s) 975
MscI TGGCCA 1 cut(s) 224
MseI TTAA 3 cut(s) 429, 693, 747
Msp20I TGGCCA 1 cut(s) 224
MspA1I CMGCKG 2 cut(s) 397, 525
MspI CCGG 3 cut(s) 282, 557, 991
MspR9I CCNGG 4 cut(s) 282, 558, 825, 992
MunI CAATTG 1 cut(s) 652
Mva1269I GAATGC 1 cut(s) 1002
MvaI CCWGG 1 cut(s) 825
MvnI CGCG 1 cut(s) 397
MwoI GCNNNNNNNGC 3 cut(s) 829, 838, 1043
NarI GGCGCC 1 cut(s) 392
NciI CCSGG 3 cut(s) 282, 558, 992
NdeII GATC 2 cut(s) 854, 1022
NlaIII CATG 2 cut(s) 883, 1095
NlaIV GGNNCC 5 cut(s) 211, 393, 822, 1019, 1097
NmuCI GTSAC 3 cut(s) 607, 631, 1100
PaqCI CACCTGC 1 cut(s) 1037
PciSI GCTCTTC 2 cut(s) 228, 587
PcsI WCGNNNNNNNCGW 4 cut(s) 192, 282, 294, 315
PctI GAATGC 1 cut(s) 1002
PdmI GAANNNNTTC 1 cut(s) 975
PfeI GAWTC 2 cut(s) 46, 870
PflMI CCANNNNNTGG 1 cut(s) 880
PfoI TCCNGGA 1 cut(s) 556
PkrI GCNGC 8 cut(s) 152, 175, 396, 664, 843, 1045, 1111, 1126
PluTI GGCGCC 1 cut(s) 395
PshAI GACNNNNGTC 1 cut(s) 1094
Psp6I CCWGG 1 cut(s) 823
PspFI CCCAGC 4 cut(s) 205, 387, 525, 1174
PspGI CCWGG 1 cut(s) 823
PspN4I GGNNCC 5 cut(s) 211, 393, 822, 1019, 1097
PspPI GGNCC 1 cut(s) 1096
PvuII CAGCTG 1 cut(s) 525
RsaI GTAC 2 cut(s) 949, 1019
RsaNI GTAC 2 cut(s) 948, 1018
SacII CCGCGG 1 cut(s) 398
SapI GCTCTTC 2 cut(s) 228, 587
SaqAI TTAA 3 cut(s) 429, 693, 747
SatI GCNGC 8 cut(s) 151, 174, 395, 663, 842, 1044, 1110, 1125
Sau3AI GATC 2 cut(s) 854, 1022
Sau96I GGNCC 1 cut(s) 1096
ScrFI CCNGG 4 cut(s) 282, 558, 825, 992
SduI GDGCHC 1 cut(s) 240
SfaNI GCATC 1 cut(s) 502
SfcI CTRYAG 2 cut(s) 24, 433
SfoI GGCGCC 1 cut(s) 393
Sfr303I CCGCGG 1 cut(s) 398
SgrBI CCGCGG 1 cut(s) 398
SinI GGWCC 1 cut(s) 1096
SmlI CTYRAG 1 cut(s) 790
SmoI CTYRAG 1 cut(s) 790
Sse9I AATT 3 cut(s) 402, 492, 652
SsiI CCGC 8 cut(s) 140, 151, 173, 240, 395, 397, 539, 720
SspDI GGCGCC 1 cut(s) 391
SspI AATATT 1 cut(s) 1120
SspMI CTAG 1 cut(s) 708
StyD4I CCNGG 4 cut(s) 280, 556, 823, 990
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 4 cut(s) 196, 377, 679, 703
TaiI ACGT 1 cut(s) 88
TaqI TCGA 3 cut(s) 276, 297, 1152
TasI AATT 3 cut(s) 402, 492, 652
TauI GCSGC 3 cut(s) 153, 176, 397
TfiI GAWTC 2 cut(s) 46, 870
Tru1I TTAA 3 cut(s) 429, 693, 747
Tru9I TTAA 3 cut(s) 429, 693, 747
TseFI GTSAC 3 cut(s) 607, 631, 1100
TseI GCWGC 5 cut(s) 662, 841, 1043, 1109, 1124
Tsp45I GTSAC 3 cut(s) 607, 631, 1100
TspDTI ATGAA 2 cut(s) 103, 435
TspGWI ACGGA 4 cut(s) 345, 396, 547, 1088
Van91I CCANNNNNTGG 1 cut(s) 880
VpaK11BI GGWCC 1 cut(s) 1096
XagI CCTNNNNNAGG 1 cut(s) 640
XmnI GAANNNNTTC 1 cut(s) 975
XspI CTAG 1 cut(s) 708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.