FvH4_5g01680

iq-domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0010781
Physical Location & Seq
Forward (+)
1081521 .. 1087086
5566 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g01680.t3

Sequence Viewer

Length: 1407 bp
ATGGGTAAAGCCTCCAGATGGTTCCGCAGCGTCCTCGGCCTCAAAAAGCCCGATAAGCCCCCCACCACCACCTCATCCTCCCCCAAACCGCCCCCCACCAAACGCCGATGGAGCTTCGTCAAGTCCTACCGAGAAAAAGACCACCGCAACGACACAACCACCGACCAGCAGTCTTCCACAAACGACGTCGTTGATCCTAACAAGCACGCGATCGCCGTAGCGGCCGCGACAGCTGCAGTAGCCGAAGCCGCAGTTGCTGCGGCACAAGCCGCCGCCGCTGTCGTCCGCCTCACCAGCAGCGGACGCTGCCCCAACAACAGCAACAACAACAACAACGTTGCCGGAGGAAGCATTGGGATTCGTGAAGAGTGGGCGGCTGTGAGAATCCAAGCGGCGTTCCGTGGCTGTTTGGCAAGGAGAGCGTTGCGTGCATTGAAAGGACTGGTGAGGCTTCAAGCACTGGTTAGAGGTCACATTGAGAGGAAGAAGTGGGCTAAGCGGTTGCAGCGGTTTCAAGCAATACTGCGAGCTCAGGCCAGAGCTCGAGCAGGGCGGACCCAGATATCAGAGCTTTCACATTCATATACAAGTTCCCAGTCTTCTCAGATTCACCAACCTGGTCCTGCAACCCCAGAAAAATTTGAACATGGACTTCGATCCAGGAGTACTAAACATGAACATTCATCAATACTGCGGAGAAATAGCTCAAGATCAAATGGTAGGATTGTTGGTGATTATGACAAAACTCACTCGGAACGATCATGGGAGCAACCAGGGTCTGCAATGAAAAATGGTACTCCTGAGGATGAGAAGAACGATAGGGTCCTTGAAATCGATACTGGGAAATCACACATTGCAGCTAAGCGGAGGAACCTCTTCCACTCTACTCATCTTGCTTTGGCTTCAGACCAATACAGCCATAGCTTCACTACATCAAAGGACTCAACAACTCATCAGACTCTTCCAAGTCCGTCTTCTTGTGAAGTCCAATCTTTAACCCCATTGAAATTCTCTAGAGAGGTCGAGGAAGACTCATTCTGCACCGCTAACAATAGCCCACAGTTTTATTCTGCTACTTCATCAAGGGGTGGTAATTCAAAGCGGACTCCTTTTACTCCCACAAAGAGCGATGGCTCAAAAAATTACCTAAGTGGGTACTCAGATTACCCGAACTATATGGCATGCACTCAATCTTCAAAGGCTAAGCTGAGGTCACTTAGCGCTCCGAAACAGAGGCCTCAGTATGAGAGGTCTATTTCAGCAAAGAGGTGCTCTGTTCCTGGCTACAGCGAATCAAGATCAAATGCACAAAGGAGTTCAGCCTTGCATTCAAACTTCACAAATAAAGCCTACCCAGGATCTGGCCGCTTGGACAGGCTTGGCATGCCTATAGGTTACAGATACTAA

Protein Analysis

469

Amino Acids

51.65

Weight (kDa)

10.34

Isoelectric Point (pI)

63.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 224 - 315 2.2e-11 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010781)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23060
fragaria_vesca FvH4_5g01680 FvH4_5g01680 FvH4_5g01680
malus_domestica MD04G1065600.v1.1
prunus_persica Prupe.5G092600_v2.0.a1
pyrus_communis pycom04g05910 pycom06g07330
rosa_chinensis RchiOBHm_Chr7g0199041
rosa_laevigata RLG00000003820
rosa_multiflora Rmu_co8081240.1_g000001 Rmu_sc0006266.1_g000033 Rmu_sc0007328.1_g000001
rosa_roxburghii Rroxscaffold_3G00257190
rosa_rugosa Rorug07G0052200
rosa_samantha Rh7AG175400 Rh7BG178800 Rh7CG184600 Rh7DG178200
rosa_wichuraiana Rw7G015390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 189
AccB7I CCANNNNNTGG 1 cut(s) 1361
AccII CGCG 2 cut(s) 209, 227
AclI AACGTT 1 cut(s) 336
AclWI GGATC 3 cut(s) 188, 651, 1366
AcoI YGGCCR 2 cut(s) 222, 1363
AcsI RAATTY 2 cut(s) 638, 1007
AcuI CTGAAG 1 cut(s) 888
AcyI GRCGYC 1 cut(s) 186
AfaI GTAC 3 cut(s) 667, 796, 1157
AfeI AGCGCT 1 cut(s) 1222
AfiI CCNNNNNNNGG 2 cut(s) 18, 1361
AgsI TTSAA 9 cut(s) 436, 455, 515, 644, 830, 1006, 1098, 1197, 1332
AhdI GACNNNNNGTC 1 cut(s) 169
AjnI CCWGG 5 cut(s) 616, 659, 772, 1279, 1354
AjuI GAANNNNNNNTTGG 2 cut(s) 958, 990
AluBI AGCT 9 cut(s) 114, 233, 530, 542, 571, 705, 860, 924, 1207
AluI AGCT 9 cut(s) 114, 233, 530, 542, 571, 705, 860, 924, 1207
Alw21I GWGCWC 3 cut(s) 532, 544, 1274
AlwI GGATC 3 cut(s) 188, 651, 1366
AlwNI CAGNNNCTG 3 cut(s) 257, 779, 1361
Ama87I CYCGRG 1 cut(s) 543
Aor51HI AGCGCT 1 cut(s) 1222
AoxI GGCC 5 cut(s) 37, 222, 534, 1235, 1363
ApeKI GCWGC 7 cut(s) 27, 233, 257, 297, 306, 505, 857
ApoI RAATTY 2 cut(s) 638, 1007
AsiSI GCGATCGC 1 cut(s) 213
Asp700I GAANNNNTTC 1 cut(s) 875
AspLEI GCGC 1 cut(s) 1223
AspS9I GGNCC 3 cut(s) 555, 620, 823
AsuHPI GGTGA 4 cut(s) 283, 457, 602, 743
AvaI CYCGRG 1 cut(s) 543
AvaII GGWCC 3 cut(s) 555, 620, 823
AxyI CCTNAGG 1 cut(s) 801
BanII GRGCYC 2 cut(s) 532, 544
BbsI GAAGAC 4 cut(s) 165, 591, 966, 1035
Bbv12I GWGCWC 3 cut(s) 532, 544, 1274
BbvCI CCTCAGC 1 cut(s) 1208
BbvI GCAGC 7 cut(s) 39, 220, 244, 293, 309, 517, 869
BccI CCATC 3 cut(s) 12, 102, 1124
BceAI ACGGC 1 cut(s) 200
BcgI CGANNNNNNTGC 2 cut(s) 16, 50
BciT130I CCWGG 5 cut(s) 618, 661, 774, 1281, 1356
BfaI CTAG 1 cut(s) 1014
BfmI CTRYAG 3 cut(s) 234, 1285, 1389
BfoI RGCGCY 1 cut(s) 1224
BglI GCCNNNNNGGC 1 cut(s) 221
BlpI GCTNAGC 3 cut(s) 495, 861, 1203
BmcAI AGTACT 1 cut(s) 667
Bme1390I CCNGG 5 cut(s) 618, 661, 774, 1281, 1356
Bme18I GGWCC 3 cut(s) 555, 620, 823
BmeRI GACNNNNNGTC 1 cut(s) 169
BmeT110I CYCGRG 1 cut(s) 543
BmgT120I GGNCC 3 cut(s) 555, 620, 823
BmiI GGNNCC 4 cut(s) 23, 557, 824, 872
BmrFI CCNGG 5 cut(s) 618, 661, 774, 1281, 1356
BmrI ACTGGG 2 cut(s) 589, 849
BmuI ACTGGG 2 cut(s) 589, 849
BpiI GAAGAC 4 cut(s) 165, 591, 966, 1035
BplI GAGNNNNNCTC 2 cut(s) 1016, 1048
Bpu10I CCTNAGC 2 cut(s) 531, 1208
Bpu1102I GCTNAGC 3 cut(s) 495, 861, 1203
BpuEI CTTGAG 1 cut(s) 691
Bsa29I ATCGAT 1 cut(s) 834
BsaHI GRCGYC 1 cut(s) 186
BsaJI CCNNGG 4 cut(s) 34, 400, 773, 1354
Bsc4I CCNNNNNNNGG 2 cut(s) 18, 1361
Bse1I ACTGG 4 cut(s) 447, 465, 595, 844
Bse21I CCTNAGG 1 cut(s) 801
Bse3DI GCAATG 2 cut(s) 789, 852
BseBI CCWGG 5 cut(s) 618, 661, 774, 1281, 1356
BseCI ATCGAT 1 cut(s) 834
BseDI CCNNGG 4 cut(s) 34, 400, 773, 1354
BseGI GGATG 2 cut(s) 74, 811
BseLI CCNNNNNNNGG 2 cut(s) 18, 1361
BseMI GCAATG 2 cut(s) 789, 852
BseMII CTCAG 6 cut(s) 545, 617, 792, 1173, 1199, 1253
BseNI ACTGG 4 cut(s) 447, 465, 595, 844
BseX3I CGGCCG 1 cut(s) 222
BseXI GCAGC 7 cut(s) 39, 220, 244, 293, 309, 517, 869
BsgI GTGCAG 1 cut(s) 1024
Bsh1236I CGCG 2 cut(s) 209, 227
Bsh1285I CGRYCG 2 cut(s) 213, 225
BshFI GGCC 5 cut(s) 39, 224, 536, 1237, 1365
BshVI ATCGAT 1 cut(s) 834
BsiEI CGRYCG 2 cut(s) 213, 225
BsiHKAI GWGCWC 3 cut(s) 532, 544, 1274
BsiHKCI CYCGRG 1 cut(s) 543
BsiSI CCGG 1 cut(s) 342
BslI CCNNNNNNNGG 2 cut(s) 18, 1361
BsmI GAATGC 1 cut(s) 1327
BsnI GGCC 5 cut(s) 39, 224, 536, 1237, 1365
BsoBI CYCGRG 1 cut(s) 543
Bsp1286I GDGCHC 3 cut(s) 532, 544, 1274
Bsp143I GATC 7 cut(s) 193, 210, 656, 710, 758, 1298, 1358
Bsp1720I GCTNAGC 3 cut(s) 495, 861, 1203
BspANI GGCC 5 cut(s) 39, 224, 536, 1237, 1365
BspCNI CTCAG 6 cut(s) 544, 616, 793, 1172, 1200, 1252
BspDI ATCGAT 1 cut(s) 834
BspFNI CGCG 2 cut(s) 209, 227
BspLI GGNNCC 4 cut(s) 23, 557, 824, 872
BspMAI CTGCAG 1 cut(s) 238
BspPI GGATC 3 cut(s) 188, 651, 1366
BsrDI GCAATG 2 cut(s) 789, 852
BsrI ACTGG 4 cut(s) 447, 465, 595, 844
BssECI CCNNGG 4 cut(s) 34, 400, 773, 1354
BssMI GATC 7 cut(s) 193, 210, 656, 710, 758, 1298, 1358
BssNI GRCGYC 1 cut(s) 186
Bst2UI CCWGG 5 cut(s) 618, 661, 774, 1281, 1356
Bst4CI ACNGT 1 cut(s) 1062
Bst6I CTCTTC 3 cut(s) 360, 881, 966
BstACI GRCGYC 1 cut(s) 186
BstAPI GCANNNNNTGC 1 cut(s) 257
BstC8I GCNNGC 5 cut(s) 207, 429, 528, 1183, 1385
BstDSI CCRYGG 1 cut(s) 400
BstF5I GGATG 2 cut(s) 74, 811
BstFNI CGCG 2 cut(s) 209, 227
BstH2I RGCGCY 1 cut(s) 1224
BstHHI GCGC 1 cut(s) 1223
BstKTI GATC 7 cut(s) 196, 213, 659, 713, 761, 1301, 1361
BstMBI GATC 7 cut(s) 193, 210, 656, 710, 758, 1298, 1358
BstMCI CGRYCG 2 cut(s) 213, 225
BstNI CCWGG 5 cut(s) 618, 661, 774, 1281, 1356
BstNSI RCATGY 2 cut(s) 1185, 1387
BstSCI CCNGG 5 cut(s) 616, 659, 772, 1279, 1354
BstSFI CTRYAG 3 cut(s) 234, 1285, 1389
BstUI CGCG 2 cut(s) 209, 227
BstV1I GCAGC 7 cut(s) 39, 220, 244, 293, 309, 517, 869
BstV2I GAAGAC 4 cut(s) 165, 591, 966, 1035
BstX2I RGATCY 1 cut(s) 1358
BstYI RGATCY 1 cut(s) 1358
BstZI CGGCCG 1 cut(s) 222
Bsu15I ATCGAT 1 cut(s) 834
Bsu36I CCTNAGG 1 cut(s) 801
BsuRI GGCC 5 cut(s) 39, 224, 536, 1237, 1365
BsuTUI ATCGAT 1 cut(s) 834
BtgI CCRYGG 1 cut(s) 400
BtgZI GCGATG 1 cut(s) 1143
BtsCI GGATG 2 cut(s) 74, 811
BtsIMutI CAGTG 1 cut(s) 458
Cac8I GCNNGC 5 cut(s) 207, 429, 528, 1183, 1385
CaiI CAGNNNCTG 3 cut(s) 257, 779, 1361
CciNI GCGGCCGC 1 cut(s) 222
CfoI GCGC 1 cut(s) 1223
Cfr13I GGNCC 3 cut(s) 555, 620, 823
ClaI ATCGAT 1 cut(s) 834
CseI GACGC 2 cut(s) 19, 312
CsiI ACCWGGT 1 cut(s) 616
Csp6I GTAC 3 cut(s) 666, 795, 1156
CviAII CATG 5 cut(s) 647, 674, 762, 1182, 1384
CviQI GTAC 3 cut(s) 666, 795, 1156
DpnI GATC 7 cut(s) 195, 212, 658, 712, 760, 1300, 1360
DpnII GATC 7 cut(s) 193, 210, 656, 710, 758, 1298, 1358
DriI GACNNNNNGTC 1 cut(s) 169
EaeI YGGCCR 2 cut(s) 222, 1363
EagI CGGCCG 1 cut(s) 222
Eam1104I CTCTTC 3 cut(s) 360, 881, 966
Eam1105I GACNNNNNGTC 1 cut(s) 169
EarI CTCTTC 3 cut(s) 360, 881, 966
EciI GGCGGA 2 cut(s) 275, 568
Ecl136II GAGCTC 2 cut(s) 530, 542
EclXI CGGCCG 1 cut(s) 222
Eco147I AGGCCT 1 cut(s) 1237
Eco24I GRGCYC 2 cut(s) 532, 544
Eco32I GATATC 1 cut(s) 564
Eco47I GGWCC 3 cut(s) 555, 620, 823
Eco47III AGCGCT 1 cut(s) 1222
Eco52I CGGCCG 1 cut(s) 222
Eco53kI GAGCTC 2 cut(s) 530, 542
Eco57I CTGAAG 1 cut(s) 888
Eco81I CCTNAGG 1 cut(s) 801
Eco88I CYCGRG 1 cut(s) 543
EcoICRI GAGCTC 2 cut(s) 530, 542
EcoO109I RGGNCCY 1 cut(s) 823
EcoRII CCWGG 5 cut(s) 616, 659, 772, 1279, 1354
EcoRV GATATC 1 cut(s) 564
EcoT38I GRGCYC 2 cut(s) 532, 544
FaeI CATG 5 cut(s) 650, 677, 765, 1185, 1387
FalI AAGNNNNNCTT 2 cut(s) 958, 990
FatI CATG 5 cut(s) 646, 673, 761, 1181, 1383
FokI GGATG 2 cut(s) 61, 818
FriOI GRGCYC 2 cut(s) 532, 544
FspBI CTAG 1 cut(s) 1014
GlaI GCGC 1 cut(s) 1222
HaeII RGCGCY 1 cut(s) 1224
HaeIII GGCC 5 cut(s) 39, 224, 536, 1237, 1365
HapII CCGG 1 cut(s) 342
HgaI GACGC 2 cut(s) 19, 312
HhaI GCGC 1 cut(s) 1223
Hin1I GRCGYC 1 cut(s) 186
Hin1II CATG 5 cut(s) 650, 677, 765, 1185, 1387
Hin6I GCGC 1 cut(s) 1221
HinP1I GCGC 1 cut(s) 1221
HinfI GANTC 8 cut(s) 358, 384, 607, 941, 958, 1031, 1105, 1292
HpaII CCGG 1 cut(s) 342
HphI GGTGA 4 cut(s) 283, 457, 602, 743
Hpy188I TCNGA 7 cut(s) 568, 606, 754, 907, 957, 1162, 1227
Hpy188III TCNNGA 6 cut(s) 15, 362, 708, 800, 1014, 1296
Hpy99I CGWCG 2 cut(s) 188, 191
HpyCH4III ACNGT 1 cut(s) 1062
HpyCH4IV ACGT 2 cut(s) 186, 336
HpySE526I ACGT 2 cut(s) 186, 336
Hsp92I GRCGYC 1 cut(s) 186
Hsp92II CATG 5 cut(s) 650, 677, 765, 1185, 1387
HspAI GCGC 1 cut(s) 1221
Kzo9I GATC 7 cut(s) 193, 210, 656, 710, 758, 1298, 1358
LmnI GCTCC 3 cut(s) 111, 766, 1228
Lsp1109I GCAGC 7 cut(s) 39, 220, 244, 293, 309, 517, 869
MabI ACCWGGT 1 cut(s) 616
MaeI CTAG 1 cut(s) 1014
MaeII ACGT 2 cut(s) 186, 336
MaeIII GTNAC 3 cut(s) 470, 1212, 1394
MalI GATC 7 cut(s) 195, 212, 658, 712, 760, 1300, 1360
MboI GATC 7 cut(s) 193, 210, 656, 710, 758, 1298, 1358
MflI RGATCY 1 cut(s) 1358
MhlI GDGCHC 3 cut(s) 532, 544, 1274
MluCI AATT 4 cut(s) 638, 1007, 1093, 1141
MlyI GAGTC 4 cut(s) 935, 952, 1025, 1099
MroXI GAANNNNTTC 1 cut(s) 875
MseI TTAA 1 cut(s) 995
MspA1I CMGCKG 4 cut(s) 233, 278, 300, 508
MspI CCGG 1 cut(s) 342
MspR9I CCNGG 5 cut(s) 618, 661, 774, 1281, 1356
Mva1269I GAATGC 1 cut(s) 1327
MvaI CCWGG 5 cut(s) 618, 661, 774, 1281, 1356
MvnI CGCG 2 cut(s) 209, 227
NdeII GATC 7 cut(s) 193, 210, 656, 710, 758, 1298, 1358
NlaIII CATG 5 cut(s) 650, 677, 765, 1185, 1387
NlaIV GGNNCC 4 cut(s) 23, 557, 824, 872
NmeAIII GCCGAG 1 cut(s) 15
NmuCI GTSAC 2 cut(s) 470, 1212
NotI GCGGCCGC 1 cut(s) 222
NspI RCATGY 2 cut(s) 1185, 1387
PaeI GCATGC 2 cut(s) 1185, 1387
PaeR7I CTCGAG 1 cut(s) 543
PceI AGGCCT 1 cut(s) 1237
PcsI WCGNNNNNNNCGW 1 cut(s) 213
PctI GAATGC 1 cut(s) 1327
PdmI GAANNNNTTC 1 cut(s) 875
PfeI GAWTC 4 cut(s) 358, 384, 607, 1292
PflMI CCANNNNNTGG 1 cut(s) 1361
PfoI TCCNGGA 1 cut(s) 659
Ple19I CGATCG 1 cut(s) 213
PleI GAGTC 4 cut(s) 935, 952, 1025, 1099
PpsI GAGTC 4 cut(s) 935, 952, 1025, 1099
PpuMI RGGWCCY 1 cut(s) 823
Psp124BI GAGCTC 2 cut(s) 532, 544
Psp1406I AACGTT 1 cut(s) 336
Psp5II RGGWCCY 1 cut(s) 823
Psp6I CCWGG 5 cut(s) 616, 659, 772, 1279, 1354
PspGI CCWGG 5 cut(s) 616, 659, 772, 1279, 1354
PspN4I GGNNCC 4 cut(s) 23, 557, 824, 872
PspPI GGNCC 3 cut(s) 555, 620, 823
PspPPI RGGWCCY 1 cut(s) 823
PspXI VCTCGAGB 1 cut(s) 543
PstI CTGCAG 1 cut(s) 238
PstNI CAGNNNCTG 3 cut(s) 257, 779, 1361
PsuI RGATCY 1 cut(s) 1358
PvuI CGATCG 1 cut(s) 213
PvuII CAGCTG 1 cut(s) 233
RgaI GCGATCGC 1 cut(s) 213
RsaI GTAC 3 cut(s) 667, 796, 1157
RsaNI GTAC 3 cut(s) 666, 795, 1156
SacI GAGCTC 2 cut(s) 532, 544
SaqAI TTAA 1 cut(s) 995
Sau3AI GATC 7 cut(s) 193, 210, 656, 710, 758, 1298, 1358
Sau96I GGNCC 3 cut(s) 555, 620, 823
ScaI AGTACT 1 cut(s) 667
SchI GAGTC 4 cut(s) 935, 952, 1025, 1099
ScrFI CCNGG 5 cut(s) 618, 661, 774, 1281, 1356
SduI GDGCHC 3 cut(s) 532, 544, 1274
SexAI ACCWGGT 1 cut(s) 616
SfaAI GCGATCGC 1 cut(s) 213
SfcI CTRYAG 3 cut(s) 234, 1285, 1389
Sfr274I CTCGAG 1 cut(s) 543
SgfI GCGATCGC 1 cut(s) 213
SinI GGWCC 3 cut(s) 555, 620, 823
SlaI CTCGAG 1 cut(s) 543
SmlI CTYRAG 2 cut(s) 543, 706
SmoI CTYRAG 2 cut(s) 543, 706
SphI GCATGC 2 cut(s) 1185, 1387
Sse9I AATT 4 cut(s) 638, 1007, 1093, 1141
SseBI AGGCCT 1 cut(s) 1237
SspMI CTAG 1 cut(s) 1014
SstI GAGCTC 2 cut(s) 532, 544
StuI AGGCCT 1 cut(s) 1237
StyD4I CCNGG 5 cut(s) 616, 659, 772, 1279, 1354
TaaI ACNGT 1 cut(s) 1062
TaiI ACGT 2 cut(s) 189, 339
TaqI TCGA 4 cut(s) 544, 655, 834, 1023
TasI AATT 4 cut(s) 638, 1007, 1093, 1141
TatI WGTACW 1 cut(s) 665
TfiI GAWTC 4 cut(s) 358, 384, 607, 1292
Tru1I TTAA 1 cut(s) 995
Tru9I TTAA 1 cut(s) 995
TscAI CASTG 1 cut(s) 465
TseFI GTSAC 2 cut(s) 470, 1212
TseI GCWGC 7 cut(s) 27, 233, 257, 297, 306, 505, 857
Tsp45I GTSAC 2 cut(s) 470, 1212
TspDTI ATGAA 5 cut(s) 570, 672, 690, 800, 1068
TspGWI ACGGA 2 cut(s) 389, 960
TspRI CASTG 1 cut(s) 465
Van91I CCANNNNNTGG 1 cut(s) 1361
VpaK11BI GGWCC 3 cut(s) 555, 620, 823
XapI RAATTY 2 cut(s) 638, 1007
XbaI TCTAGA 1 cut(s) 1013
XceI RCATGY 2 cut(s) 1185, 1387
XhoI CTCGAG 1 cut(s) 543
XmnI GAANNNNTTC 1 cut(s) 875
XspI CTAG 1 cut(s) 1014
ZraI GACGTC 1 cut(s) 187
ZrmI AGTACT 1 cut(s) 667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.