FvH4_5g04600

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006747
Physical Location & Seq
Forward (+)
2704915 .. 2709157
4243 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g04600.t2

Sequence Viewer

Length: 1242 bp
ATGGACCCAGCTTTCGAGGATGAGGGCCAAGGCGGTGAGACAGTCACAATCGAAGAGTATCTTAAGGATGTTGAGGAGCAGGAACTGGATGCTGATTTGGTTTTGGGGGGTGATGAAGGCAAGGAGTGTACCTATAATAATGGGTATATGAAGAGGCAAGCAATTTTCTCATGCTTGACTTGCACACCAGATGGTAATGCTGGAGTTTGCACAGCTTGCAGCTTGTCCTGTCATGATGGCCATGAGATTGTGGAACTATGGACCAAGAGAAACTTCAGATGTGATTGTGGAAATTCAAAATTTGGAGAGTTCTACTGCAAGATTTTCCCAAGTAAAGATATTGAAAATGTTGAGAACTCGTACAATCATAACTTCAAGGGTTCCTACTGTACCTGTGGCCGCCCTTATCCTGATCCAGACGTGGAAGAACAAGTAGAGATGATGCAGTGCTGCTTCTGTGAGGACTGGTTCCATGAGGAACATATCGGTCTTGATTCTTCTGATGAGCTTCCAAGGGACGGAGAAGGTGAACCTCTATATGAGGATTTTATATGTGCAGCATGCTCAAAAGTTTGCTCTTTTTTAAGACTATATACTCGAATCATCTTGGAAGCAAGGAGCCAAAAGAATGCTGCTGTTAAGACTGGAAAAGATAAAGAAGTTATAGCAGATGAAGTCCCTTTAGCCTGTGGATCTGGAAAGCTAGAAAGTATATCTCAGAGTTTGACTACCAACACTGAATCTGTGACTGGAGGGGAAGTTTCAGTTCTTGGAGAAAATTCTGGGAAGAAAATATGTTCAGGGCAATGCAGCGCAGATGCTGATGCACATGCTACTTGTGTTATTGGAGTTGATCTGGCAGTTGCTTCCCCAGCTTCGGAGAGCAAGCCAATGTTTCTTTCAAAAAACTGGCGGAGTGCTCTCTGCAGGTGTGAAAAATGCAGTGAATATTATAAAAAGAAGCATATTAGTTTTATAATTGACAAGGAGGATTCAATCATTGAGTATGAGAAAATGGCAAAGCGGAAGAGAGAAGAGAAGTTGCAGCAACGTGACGGGGCAGAGTTAAGTATGCTGGATAACCTTGGCCATGTAGAGAAAATTGAGATTCTGAATGGCATTGCAGATATCAAAGATGAGCTTTATTCCTTCCTGGGATCATTTGATTCATCGAAGGCAATCACATCTGCTGATGTCCACCAGATTTTTGAGAATCTAAAGAAACGCAGGCGTGTAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-UBR

Protein Analysis

414

Amino Acids

46.1

Weight (kDa)

4.79

Isoelectric Point (pI)

50.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-UBR PF02207 43 - 107 2e-13 Putative zinc finger in N-recognin (UBR box)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 954, 977
AarI CACCTGC 1 cut(s) 918
Acc36I ACCTGC 1 cut(s) 918
AciI CCGC 4 cut(s) 33, 400, 913, 1024
AclWI GGATC 3 cut(s) 407, 700, 1165
AcoI YGGCCR 3 cut(s) 238, 397, 1087
AcsI RAATTY 3 cut(s) 292, 299, 778
AcuI CTGAAG 1 cut(s) 259
AfaI GTAC 3 cut(s) 130, 362, 391
AfiI CCNNNNNNNGG 2 cut(s) 518, 877
AflII CTTAAG 1 cut(s) 62
AgsI TTSAA 5 cut(s) 297, 344, 376, 903, 996
AjiI CACGTC 1 cut(s) 421
AjnI CCWGG 1 cut(s) 1152
AjuI GAANNNNNNNTTGG 4 cut(s) 257, 289, 883, 915
AluBI AGCT 7 cut(s) 11, 215, 222, 508, 703, 875, 1141
AluI AGCT 7 cut(s) 11, 215, 222, 508, 703, 875, 1141
Alw21I GWGCWC 1 cut(s) 922
Alw26I GTCTC 1 cut(s) 32
AlwI GGATC 3 cut(s) 407, 700, 1165
AlwNI CAGNNNCTG 2 cut(s) 85, 821
AoxI GGCC 4 cut(s) 25, 238, 397, 1087
ApeKI GCWGC 6 cut(s) 219, 450, 557, 632, 810, 1045
ApoI RAATTY 3 cut(s) 292, 299, 778
ArsI GACNNNNNNTTYG 1 cut(s) 28
AspLEI GCGC 1 cut(s) 815
AspS9I GGNCC 3 cut(s) 4, 25, 261
AsuHPI GGTGA 3 cut(s) 47, 122, 539
AvaII GGWCC 2 cut(s) 4, 261
BalI TGGCCA 2 cut(s) 240, 1089
Bbv12I GWGCWC 1 cut(s) 922
BbvI GCAGC 6 cut(s) 231, 437, 569, 619, 822, 1057
BccI CCATC 2 cut(s) 185, 230
BciT130I CCWGG 1 cut(s) 1154
BcoDI GTCTC 1 cut(s) 32
BfaI CTAG 1 cut(s) 704
BfmI CTRYAG 1 cut(s) 925
BfrI CTTAAG 1 cut(s) 62
BfuAI ACCTGC 1 cut(s) 918
BisI GCNGC 7 cut(s) 220, 400, 451, 558, 633, 811, 1046
BlsI GCNGC 7 cut(s) 221, 401, 452, 559, 634, 812, 1047
Bme1390I CCNGG 1 cut(s) 1154
Bme18I GGWCC 2 cut(s) 4, 261
BmgBI CACGTC 1 cut(s) 421
BmgT120I GGNCC 3 cut(s) 4, 25, 261
BmiI GGNNCC 4 cut(s) 6, 382, 470, 620
BmrFI CCNGG 1 cut(s) 1154
BmsI GCATC 4 cut(s) 79, 432, 808, 814
BpmI CTGGAG 2 cut(s) 222, 771
BsaJI CCNNGG 4 cut(s) 28, 512, 1084, 1153
BsaXI ACNNNNNCTCC 2 cut(s) 744, 774
Bsc4I CCNNNNNNNGG 2 cut(s) 518, 877
Bse1I ACTGG 5 cut(s) 90, 470, 649, 754, 914
Bse3DI GCAATG 2 cut(s) 812, 1119
BseBI CCWGG 1 cut(s) 1154
BseDI CCNNGG 4 cut(s) 28, 512, 1084, 1153
BseGI GGATG 3 cut(s) 25, 73, 94
BseLI CCNNNNNNNGG 2 cut(s) 518, 877
BseMI GCAATG 2 cut(s) 812, 1119
BseMII CTCAG 1 cut(s) 731
BseNI ACTGG 5 cut(s) 90, 470, 649, 754, 914
BseRI GAGGAG 1 cut(s) 89
BseXI GCAGC 6 cut(s) 231, 437, 569, 619, 822, 1057
BseYI CCCAGC 2 cut(s) 7, 871
BsgI GTGCAG 1 cut(s) 576
BshFI GGCC 4 cut(s) 27, 240, 399, 1089
BsiHKAI GWGCWC 1 cut(s) 922
BslFI GGGAC 2 cut(s) 530, 662
BslI CCNNNNNNNGG 2 cut(s) 518, 877
BsmAI GTCTC 1 cut(s) 32
BsmFI GGGAC 2 cut(s) 530, 662
BsmI GAATGC 1 cut(s) 634
BsnI GGCC 4 cut(s) 27, 240, 399, 1089
Bsp1286I GDGCHC 1 cut(s) 922
Bsp143I GATC 4 cut(s) 412, 692, 853, 1157
BspACI CCGC 4 cut(s) 33, 400, 913, 1024
BspANI GGCC 4 cut(s) 27, 240, 399, 1089
BspCNI CTCAG 1 cut(s) 730
BspHI TCATGA 1 cut(s) 232
BspLI GGNNCC 4 cut(s) 6, 382, 470, 620
BspMAI CTGCAG 1 cut(s) 929
BspMI ACCTGC 1 cut(s) 918
BspPI GGATC 3 cut(s) 407, 700, 1165
BspTI CTTAAG 1 cut(s) 62
BsrDI GCAATG 2 cut(s) 812, 1119
BsrI ACTGG 5 cut(s) 90, 470, 649, 754, 914
BssECI CCNNGG 4 cut(s) 28, 512, 1084, 1153
BssMI GATC 4 cut(s) 412, 692, 853, 1157
BssT1I CCWWGG 3 cut(s) 28, 512, 1084
Bst2UI CCWGG 1 cut(s) 1154
Bst4CI ACNGT 2 cut(s) 43, 389
Bst6I CTCTTC 4 cut(s) 48, 146, 1022, 1029
BstAFI CTTAAG 1 cut(s) 62
BstAPI GCANNNNNTGC 1 cut(s) 216
BstC8I GCNNGC 5 cut(s) 159, 217, 562, 887, 1229
BstDEI CTNAG 1 cut(s) 717
BstF5I GGATG 3 cut(s) 25, 73, 94
BstHHI GCGC 1 cut(s) 815
BstKTI GATC 4 cut(s) 415, 695, 856, 1160
BstMAI GTCTC 1 cut(s) 32
BstMBI GATC 4 cut(s) 412, 692, 853, 1157
BstMWI GCNNNNNNNGC 3 cut(s) 180, 216, 872
BstNI CCWGG 1 cut(s) 1154
BstNSI RCATGY 2 cut(s) 564, 833
BstSCI CCNGG 1 cut(s) 1152
BstSFI CTRYAG 1 cut(s) 925
BstV1I GCAGC 6 cut(s) 231, 437, 569, 619, 822, 1057
BstX2I RGATCY 1 cut(s) 692
BstYI RGATCY 1 cut(s) 692
BsuRI GGCC 4 cut(s) 27, 240, 399, 1089
BtrI CACGTC 1 cut(s) 421
BtsCI GGATG 3 cut(s) 25, 73, 94
BtsI GCAGTG 2 cut(s) 452, 949
BtsIMutI CAGTG 3 cut(s) 452, 735, 949
BveI ACCTGC 1 cut(s) 918
Cac8I GCNNGC 5 cut(s) 159, 217, 562, 887, 1229
CaiI CAGNNNCTG 2 cut(s) 85, 821
CciI TCATGA 1 cut(s) 232
CfoI GCGC 1 cut(s) 815
Cfr13I GGNCC 3 cut(s) 4, 25, 261
Csp6I GTAC 3 cut(s) 129, 361, 390
CviAII CATG 7 cut(s) 171, 233, 242, 473, 561, 830, 1091
CviQI GTAC 3 cut(s) 129, 361, 390
DdeI CTNAG 1 cut(s) 717
DpnI GATC 4 cut(s) 414, 694, 855, 1159
DpnII GATC 4 cut(s) 412, 692, 853, 1157
EaeI YGGCCR 3 cut(s) 238, 397, 1087
Eam1104I CTCTTC 4 cut(s) 48, 146, 1022, 1029
EarI CTCTTC 4 cut(s) 48, 146, 1022, 1029
EciI GGCGGA 1 cut(s) 928
Eco130I CCWWGG 3 cut(s) 28, 512, 1084
Eco32I GATATC 1 cut(s) 1129
Eco47I GGWCC 2 cut(s) 4, 261
Eco57I CTGAAG 1 cut(s) 259
EcoRII CCWGG 1 cut(s) 1152
EcoRV GATATC 1 cut(s) 1129
EcoT14I CCWWGG 3 cut(s) 28, 512, 1084
ErhI CCWWGG 3 cut(s) 28, 512, 1084
FaeI CATG 7 cut(s) 174, 236, 245, 476, 564, 833, 1094
FalI AAGNNNNNCTT 6 cut(s) 45, 77, 257, 289, 1125, 1157
FaqI GGGAC 2 cut(s) 530, 662
FatI CATG 7 cut(s) 170, 232, 241, 472, 560, 829, 1090
Fnu4HI GCNGC 7 cut(s) 220, 400, 451, 558, 633, 811, 1046
FokI GGATG 3 cut(s) 32, 80, 101
Fsp4HI GCNGC 7 cut(s) 220, 400, 451, 558, 633, 811, 1046
FspBI CTAG 1 cut(s) 704
GlaI GCGC 1 cut(s) 814
GluI GCNGC 7 cut(s) 220, 400, 451, 558, 633, 811, 1046
GsaI CCCAGC 2 cut(s) 11, 875
GsuI CTGGAG 2 cut(s) 222, 771
HaeIII GGCC 4 cut(s) 27, 240, 399, 1089
HhaI GCGC 1 cut(s) 815
Hin1II CATG 7 cut(s) 174, 236, 245, 476, 564, 833, 1094
Hin6I GCGC 1 cut(s) 813
HinP1I GCGC 1 cut(s) 813
HinfI GANTC 7 cut(s) 494, 600, 740, 992, 1108, 1166, 1213
HphI GGTGA 3 cut(s) 47, 122, 539
Hpy166II GTNNAC 3 cut(s) 129, 530, 1198
Hpy188I TCNGA 5 cut(s) 278, 502, 720, 880, 1113
Hpy188III TCNNGA 5 cut(s) 233, 410, 416, 491, 696
Hpy8I GTNNAC 3 cut(s) 129, 530, 1198
HpyAV CCTTC 4 cut(s) 110, 518, 1159, 1168
HpyCH4III ACNGT 2 cut(s) 43, 389
HpyCH4IV ACGT 2 cut(s) 420, 1051
HpyF10VI GCNNNNNNNGC 3 cut(s) 180, 216, 872
HpyF3I CTNAG 1 cut(s) 717
HpySE526I ACGT 2 cut(s) 420, 1051
Hsp92II CATG 7 cut(s) 174, 236, 245, 476, 564, 833, 1094
HspAI GCGC 1 cut(s) 813
Kzo9I GATC 4 cut(s) 412, 692, 853, 1157
LmnI GCTCC 2 cut(s) 76, 618
Lsp1109I GCAGC 6 cut(s) 231, 437, 569, 619, 822, 1057
LweI GCATC 4 cut(s) 79, 432, 808, 814
MaeI CTAG 1 cut(s) 704
MaeII ACGT 2 cut(s) 420, 1051
MaeIII GTNAC 3 cut(s) 43, 745, 1052
MalI GATC 4 cut(s) 414, 694, 855, 1159
MboI GATC 4 cut(s) 412, 692, 853, 1157
MboII GAAGA 7 cut(s) 65, 163, 437, 489, 799, 1039, 1046
MflI RGATCY 1 cut(s) 692
MhlI GDGCHC 1 cut(s) 922
MlsI TGGCCA 2 cut(s) 240, 1089
MluCI AATT 6 cut(s) 162, 292, 299, 778, 978, 1101
MluNI TGGCCA 2 cut(s) 240, 1089
Mox20I TGGCCA 2 cut(s) 240, 1089
MscI TGGCCA 2 cut(s) 240, 1089
MseI TTAA 4 cut(s) 63, 584, 639, 1067
Msp20I TGGCCA 2 cut(s) 240, 1089
MspCI CTTAAG 1 cut(s) 62
MspR9I CCNGG 1 cut(s) 1154
Mva1269I GAATGC 1 cut(s) 634
MvaI CCWGG 1 cut(s) 1154
MwoI GCNNNNNNNGC 3 cut(s) 180, 216, 872
NdeII GATC 4 cut(s) 412, 692, 853, 1157
NlaIII CATG 7 cut(s) 174, 236, 245, 476, 564, 833, 1094
NlaIV GGNNCC 4 cut(s) 6, 382, 470, 620
NmuCI GTSAC 3 cut(s) 43, 745, 1052
NspI RCATGY 2 cut(s) 564, 833
PaeI GCATGC 1 cut(s) 564
PagI TCATGA 1 cut(s) 232
PaqCI CACCTGC 1 cut(s) 918
PctI GAATGC 1 cut(s) 634
PfeI GAWTC 7 cut(s) 494, 600, 740, 992, 1108, 1166, 1213
PkrI GCNGC 7 cut(s) 221, 401, 452, 559, 634, 812, 1047
PsiI TTATAA 2 cut(s) 954, 977
Psp6I CCWGG 1 cut(s) 1152
PspFI CCCAGC 2 cut(s) 7, 871
PspGI CCWGG 1 cut(s) 1152
PspN4I GGNNCC 4 cut(s) 6, 382, 470, 620
PspPI GGNCC 3 cut(s) 4, 25, 261
PstI CTGCAG 1 cut(s) 929
PstNI CAGNNNCTG 2 cut(s) 85, 821
PsuI RGATCY 1 cut(s) 692
RsaI GTAC 3 cut(s) 130, 362, 391
RsaNI GTAC 3 cut(s) 129, 361, 390
SaqAI TTAA 4 cut(s) 63, 584, 639, 1067
SatI GCNGC 7 cut(s) 220, 400, 451, 558, 633, 811, 1046
Sau3AI GATC 4 cut(s) 412, 692, 853, 1157
Sau96I GGNCC 3 cut(s) 4, 25, 261
ScrFI CCNGG 1 cut(s) 1154
SduI GDGCHC 1 cut(s) 922
SfaNI GCATC 4 cut(s) 79, 432, 808, 814
SfcI CTRYAG 1 cut(s) 925
SinI GGWCC 2 cut(s) 4, 261
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
SphI GCATGC 1 cut(s) 564
Sse9I AATT 6 cut(s) 162, 292, 299, 778, 978, 1101
SsiI CCGC 4 cut(s) 33, 400, 913, 1024
SspI AATATT 1 cut(s) 950
SspMI CTAG 1 cut(s) 704
StyD4I CCNGG 1 cut(s) 1152
StyI CCWWGG 3 cut(s) 28, 512, 1084
TaaI ACNGT 2 cut(s) 43, 389
TaiI ACGT 2 cut(s) 423, 1054
TaqI TCGA 4 cut(s) 15, 51, 598, 1172
TaqII GACCGA 1 cut(s) 476
TasI AATT 6 cut(s) 162, 292, 299, 778, 978, 1101
TauI GCSGC 1 cut(s) 402
TfiI GAWTC 7 cut(s) 494, 600, 740, 992, 1108, 1166, 1213
Tru1I TTAA 4 cut(s) 63, 584, 639, 1067
Tru9I TTAA 4 cut(s) 63, 584, 639, 1067
TscAI CASTG 3 cut(s) 452, 742, 949
TseFI GTSAC 3 cut(s) 43, 745, 1052
TseI GCWGC 6 cut(s) 219, 450, 557, 632, 810, 1045
Tsp45I GTSAC 3 cut(s) 43, 745, 1052
TspDTI ATGAA 4 cut(s) 129, 164, 687, 1158
TspGWI ACGGA 1 cut(s) 534
TspRI CASTG 3 cut(s) 452, 742, 949
Vha464I CTTAAG 1 cut(s) 62
VpaK11BI GGWCC 2 cut(s) 4, 261
XapI RAATTY 3 cut(s) 292, 299, 778
XceI RCATGY 2 cut(s) 564, 833
XspI CTAG 1 cut(s) 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.