MD06G1107100.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0006747
Physical Location & Seq
Forward (+)
24650018 .. 24654174
4157 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1107100.v1.1.491

Sequence Viewer

Length: 1437 bp
ATGGCGGGAAAATGTCCTTATAGTCCTTTAACAGAAATAAAATCCCCAACCCTCTCTCTCCCTTTTCTCTCTCCGAAACCCCTCTTCCGTGCTTCAATCCCACACCCGCATCAGCATTCTCTTCGATCTCTGAGTGGCCTAATCCAAGTCGCCCGAAAAATTGGCTTTGGCCCGAAGCAATCGGTCGTCTTTGCGAAGATGGACAATGCATTCGAGGATGAGGCTGAGCACACCGTTTCGATCCAAGAGTATCTTAAGGATGTTGAGGAACAGGAACTGGAAGTTGATTTGGTTTTGGGAGGCGATGAAGGCAAGGTGTGTACCTATAATAATGGTTATATGAAGAGGCAAGCAATTTTCTCATGTCTGACATGCACCCCAGATGGGAATGCTGGAGTTTGCACAGCTTGTAGCTTGTCTTGCCATGATGGCCATGAGATTGTAGAACTGTGGACCAAAAGAAATTTTAGATGTGATTGTGGAAATTCAAAATTTGGAGAGTTCTTTTGCAAGCTTTCCCCCAATAAAGATGTAGAAAACGTTGAAAATTCTTACAATCAAAATTTCAAAGGATCATACTGCACCTGCAGTCGCCCGTATCCTGATCCAGATGTTGAAGAACAAGTAGAGATGATACAATGCTGTGTGTGTGAAGACTGGTTCCATGAGGAACACATTGGTCTTGACTCTTCTGATGAGATTCCAAGAGATGAGGAAGGTGAACCACTTTATGAGGATTTTATTTGTAAGGGATGCTCAGCAGTTTGCTCTTTTATCAGACTATACAATCAAACCATCTTGGCATCTGGCAGACAAAAGGATGCTCCCATCAAGTCTGCCAAGGATAAAGAGGTTTTAAGCGATACGCCTTTAGCTTGTGGATCTAGAAGGCTAGAGAATGATATTTCTCAAACTTGTAATACCATTTCTGAACCTGTATCTGGTGGGGATAGATTGCTCCTTGGAGAAAATTCTGGGAAGAATACAGATGCGGGTCAATGCACTACAGATGCCAATCCACCTGTTACTTGCGTTCTTGGAGTTAATCTGGTGGTTGCTTCCCCTGTTTTAGAGTGTAAGCCAATGTTTCTTTCTAAAAACTGGAGGGATGCACTCTGCAAGTGTGAAAAATGCAGTGAGTTTTACATACAGAAGCATATTAGGTTTCTGCTTGACAAGGAAGACTCGATCATGGAGTATGAGAAAATGGCAAAACTGAAGAGAGAGGAAAAATTGCAGGAACAGGAGGGGGCTGAGTTGACTATGCTCGATAAACTTGGCCATGTAGAGAAAATGGAGATTCTGAGTGGCATTGCAGACATGAAAGATGAGCTTCATTCTTTCCTGGGATCGTTTGATCCGTCGAAGACAATCACCTCTGCTGATGTCCACCAGATGTTTGAGAATCTAGCAAAGAAACGCAGGCGTGTAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-UBR

Protein Analysis

479

Amino Acids

53.59

Weight (kDa)

5.09

Isoelectric Point (pI)

49.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-UBR PF02207 107 - 172 1.1e-13 Putative zinc finger in N-recognin (UBR box)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 593
Acc36I ACCTGC 1 cut(s) 593
AciI CCGC 3 cut(s) 5, 107, 992
AclI AACGTT 1 cut(s) 540
AclWI GGATC 6 cut(s) 235, 580, 599, 889, 1352, 1357
AcoI YGGCCR 2 cut(s) 430, 1279
AcsI RAATTY 6 cut(s) 463, 484, 491, 547, 562, 970
AcuI CTGAAG 1 cut(s) 1238
AfaI GTAC 1 cut(s) 322
AfiI CCNNNNNNNGG 2 cut(s) 384, 941
AflII CTTAAG 1 cut(s) 254
AgsI TTSAA 5 cut(s) 96, 489, 545, 568, 617
AjnI CCWGG 1 cut(s) 1344
AjuI GAANNNNNNNTTGG 2 cut(s) 1075, 1107
AluBI AGCT 5 cut(s) 407, 414, 514, 875, 1333
AluI AGCT 5 cut(s) 407, 414, 514, 875, 1333
Alw21I GWGCWC 1 cut(s) 231
AlwI GGATC 6 cut(s) 235, 580, 599, 889, 1352, 1357
AlwNI CAGNNNCTG 1 cut(s) 277
AoxI GGCC 4 cut(s) 136, 169, 430, 1279
ApoI RAATTY 6 cut(s) 463, 484, 491, 547, 562, 970
ArsI GACNNNNNNTTYG 2 cut(s) 194, 226
AspS9I GGNCC 2 cut(s) 170, 453
AsuHPI GGTGA 2 cut(s) 731, 1366
AvaII GGWCC 1 cut(s) 453
BalI TGGCCA 2 cut(s) 432, 1281
BbsI GAAGAC 3 cut(s) 660, 1188, 1373
Bbv12I GWGCWC 1 cut(s) 231
BccI CCATC 5 cut(s) 193, 377, 422, 803, 836
BcgI CGANNNNNNTGC 2 cut(s) 104, 138
BciT130I CCWGG 1 cut(s) 1346
BciVI GTATCC 1 cut(s) 609
BfaI CTAG 3 cut(s) 885, 893, 1409
BfmI CTRYAG 2 cut(s) 586, 1005
BfrI CTTAAG 1 cut(s) 254
BfuAI ACCTGC 1 cut(s) 593
BfuI GTATCC 1 cut(s) 609
BglI GCCNNNNNGGC 1 cut(s) 429
BlpI GCTNAGC 2 cut(s) 225, 757
Bme1390I CCNGG 1 cut(s) 1346
Bme18I GGWCC 1 cut(s) 453
BmgT120I GGNCC 2 cut(s) 170, 453
BmiI GGNNCC 1 cut(s) 662
BmrFI CCNGG 1 cut(s) 1346
BmsI GCATC 7 cut(s) 118, 743, 811, 812, 979, 1000, 1099
BpiI GAAGAC 3 cut(s) 660, 1188, 1373
BpmI CTGGAG 2 cut(s) 414, 1123
Bpu1102I GCTNAGC 2 cut(s) 225, 757
BsaBI GATNNNNATC 1 cut(s) 1014
BsaJI CCNNGG 3 cut(s) 840, 961, 1345
Bsc4I CCNNNNNNNGG 2 cut(s) 384, 941
Bse1I ACTGG 3 cut(s) 282, 662, 1106
Bse3DI GCAATG 1 cut(s) 1311
Bse8I GATNNNNATC 1 cut(s) 1014
BseBI CCWGG 1 cut(s) 1346
BseDI CCNNGG 3 cut(s) 840, 961, 1345
BseGI GGATG 5 cut(s) 223, 265, 758, 826, 1114
BseJI GATNNNNATC 1 cut(s) 1014
BseLI CCNNNNNNNGG 2 cut(s) 384, 941
BseMI GCAATG 1 cut(s) 1311
BseMII CTCAG 5 cut(s) 122, 216, 771, 1245, 1295
BseNI ACTGG 3 cut(s) 282, 662, 1106
BsgI GTGCAG 1 cut(s) 565
Bsh1285I CGRYCG 1 cut(s) 186
BshFI GGCC 4 cut(s) 138, 171, 432, 1281
BsiEI CGRYCG 1 cut(s) 186
BsiHKAI GWGCWC 1 cut(s) 231
BslI CCNNNNNNNGG 2 cut(s) 384, 941
BsmI GAATGC 3 cut(s) 115, 209, 394
BsnI GGCC 4 cut(s) 138, 171, 432, 1281
Bsp1286I GDGCHC 1 cut(s) 231
Bsp143I GATC 8 cut(s) 125, 240, 572, 604, 881, 1188, 1349, 1357
Bsp1720I GCTNAGC 2 cut(s) 225, 757
BspACI CCGC 3 cut(s) 5, 107, 992
BspANI GGCC 4 cut(s) 138, 171, 432, 1281
BspCNI CTCAG 5 cut(s) 123, 217, 770, 1246, 1296
BspLI GGNNCC 1 cut(s) 662
BspMAI CTGCAG 1 cut(s) 590
BspMI ACCTGC 1 cut(s) 593
BspPI GGATC 6 cut(s) 235, 580, 599, 889, 1352, 1357
BspTI CTTAAG 1 cut(s) 254
BsrDI GCAATG 1 cut(s) 1311
BsrI ACTGG 3 cut(s) 282, 662, 1106
BssECI CCNNGG 3 cut(s) 840, 961, 1345
BssMI GATC 8 cut(s) 125, 240, 572, 604, 881, 1188, 1349, 1357
BssT1I CCWWGG 2 cut(s) 840, 961
Bst2UI CCWGG 1 cut(s) 1346
Bst4CI ACNGT 2 cut(s) 235, 450
Bst6I CTCTTC 5 cut(s) 89, 126, 338, 694, 1214
BstAFI CTTAAG 1 cut(s) 254
BstC8I GCNNGC 3 cut(s) 351, 512, 1424
BstDEI CTNAG 5 cut(s) 131, 225, 757, 1254, 1304
BstF5I GGATG 5 cut(s) 223, 265, 758, 826, 1114
BstKTI GATC 8 cut(s) 128, 243, 575, 607, 884, 1191, 1352, 1360
BstMBI GATC 8 cut(s) 125, 240, 572, 604, 881, 1188, 1349, 1357
BstMCI CGRYCG 1 cut(s) 186
BstMWI GCNNNNNNNGC 3 cut(s) 309, 420, 429
BstNI CCWGG 1 cut(s) 1346
BstNSI RCATGY 1 cut(s) 375
BstSCI CCNGG 1 cut(s) 1344
BstSFI CTRYAG 2 cut(s) 586, 1005
BstV2I GAAGAC 3 cut(s) 660, 1188, 1373
BstX2I RGATCY 1 cut(s) 881
BstYI RGATCY 1 cut(s) 881
BsuI GTATCC 1 cut(s) 609
BsuRI GGCC 4 cut(s) 138, 171, 432, 1281
BtgZI GCGATG 1 cut(s) 318
BtsCI GGATG 5 cut(s) 223, 265, 758, 826, 1114
BtsI GCAGTG 1 cut(s) 1141
BtsIMutI CAGTG 1 cut(s) 1141
BveI ACCTGC 1 cut(s) 593
Cac8I GCNNGC 3 cut(s) 351, 512, 1424
CaiI CAGNNNCTG 1 cut(s) 277
Cfr13I GGNCC 2 cut(s) 170, 453
Csp6I GTAC 1 cut(s) 321
CviAII CATG 8 cut(s) 363, 372, 425, 434, 665, 1192, 1283, 1321
CviQI GTAC 1 cut(s) 321
DdeI CTNAG 5 cut(s) 131, 225, 757, 1254, 1304
DpnI GATC 8 cut(s) 127, 242, 574, 606, 883, 1190, 1351, 1359
DpnII GATC 8 cut(s) 125, 240, 572, 604, 881, 1188, 1349, 1357
EaeI YGGCCR 2 cut(s) 430, 1279
Eam1104I CTCTTC 5 cut(s) 89, 126, 338, 694, 1214
EarI CTCTTC 5 cut(s) 89, 126, 338, 694, 1214
Eco130I CCWWGG 2 cut(s) 840, 961
Eco47I GGWCC 1 cut(s) 453
Eco57I CTGAAG 1 cut(s) 1238
EcoRII CCWGG 1 cut(s) 1344
EcoT14I CCWWGG 2 cut(s) 840, 961
EcoT22I ATGCAT 1 cut(s) 211
ErhI CCWWGG 2 cut(s) 840, 961
FaeI CATG 8 cut(s) 366, 375, 428, 437, 668, 1195, 1286, 1324
FalI AAGNNNNNCTT 4 cut(s) 237, 269, 1317, 1349
FatI CATG 8 cut(s) 362, 371, 424, 433, 664, 1191, 1282, 1320
FauI CCCGC 2 cut(s) 114, 985
FokI GGATG 5 cut(s) 230, 272, 765, 833, 1121
FspBI CTAG 3 cut(s) 885, 893, 1409
GsuI CTGGAG 2 cut(s) 414, 1123
HaeIII GGCC 4 cut(s) 138, 171, 432, 1281
Hin1II CATG 8 cut(s) 366, 375, 428, 437, 668, 1195, 1286, 1324
HincII GTYRAC 1 cut(s) 1260
HindII GTYRAC 1 cut(s) 1260
HindIII AAGCTT 1 cut(s) 512
HinfI GANTC 5 cut(s) 686, 700, 1184, 1300, 1405
HphI GGTGA 2 cut(s) 731, 1366
Hpy166II GTNNAC 5 cut(s) 321, 453, 722, 1260, 1390
Hpy188I TCNGA 7 cut(s) 75, 132, 369, 694, 779, 931, 1305
Hpy188III TCNNGA 4 cut(s) 602, 608, 683, 885
Hpy8I GTNNAC 5 cut(s) 321, 453, 722, 1260, 1390
Hpy99I CGWCG 1 cut(s) 1366
HpyAV CCTTC 3 cut(s) 302, 710, 882
HpyCH4III ACNGT 2 cut(s) 235, 450
HpyCH4IV ACGT 1 cut(s) 540
HpyF10VI GCNNNNNNNGC 3 cut(s) 309, 420, 429
HpyF3I CTNAG 5 cut(s) 131, 225, 757, 1254, 1304
HpySE526I ACGT 1 cut(s) 540
Hsp92II CATG 8 cut(s) 366, 375, 428, 437, 668, 1195, 1286, 1324
Kzo9I GATC 8 cut(s) 125, 240, 572, 604, 881, 1188, 1349, 1357
LmnI GCTCC 2 cut(s) 829, 963
LweI GCATC 7 cut(s) 118, 743, 811, 812, 979, 1000, 1099
MaeI CTAG 3 cut(s) 885, 893, 1409
MaeII ACGT 1 cut(s) 540
MaeIII GTNAC 1 cut(s) 1024
MalI GATC 8 cut(s) 127, 242, 574, 606, 883, 1190, 1351, 1359
MboI GATC 8 cut(s) 125, 240, 572, 604, 881, 1188, 1349, 1357
MflI RGATCY 1 cut(s) 881
MhlI GDGCHC 1 cut(s) 231
MlsI TGGCCA 2 cut(s) 432, 1281
MluCI AATT 9 cut(s) 159, 354, 463, 484, 491, 547, 562, 970, 1232
MluNI TGGCCA 2 cut(s) 432, 1281
MlyI GAGTC 2 cut(s) 680, 1178
Mox20I TGGCCA 2 cut(s) 432, 1281
Mph1103I ATGCAT 1 cut(s) 211
MscI TGGCCA 2 cut(s) 432, 1281
MseI TTAA 4 cut(s) 29, 255, 857, 1044
Msp20I TGGCCA 2 cut(s) 432, 1281
MspCI CTTAAG 1 cut(s) 254
MspR9I CCNGG 1 cut(s) 1346
Mva1269I GAATGC 3 cut(s) 115, 209, 394
MvaI CCWGG 1 cut(s) 1346
MwoI GCNNNNNNNGC 3 cut(s) 309, 420, 429
NdeII GATC 8 cut(s) 125, 240, 572, 604, 881, 1188, 1349, 1357
NlaIII CATG 8 cut(s) 366, 375, 428, 437, 668, 1195, 1286, 1324
NlaIV GGNNCC 1 cut(s) 662
NsiI ATGCAT 1 cut(s) 211
NspI RCATGY 1 cut(s) 375
PaqCI CACCTGC 1 cut(s) 593
PcsI WCGNNNNNNNCGW 1 cut(s) 1358
PctI GAATGC 3 cut(s) 115, 209, 394
PfeI GAWTC 3 cut(s) 700, 1300, 1405
PleI GAGTC 2 cut(s) 680, 1178
PpsI GAGTC 2 cut(s) 680, 1178
Psp1406I AACGTT 1 cut(s) 540
Psp6I CCWGG 1 cut(s) 1344
PspGI CCWGG 1 cut(s) 1344
PspN4I GGNNCC 1 cut(s) 662
PspPI GGNCC 2 cut(s) 170, 453
PstI CTGCAG 1 cut(s) 590
PstNI CAGNNNCTG 1 cut(s) 277
PsuI RGATCY 1 cut(s) 881
RsaI GTAC 1 cut(s) 322
RsaNI GTAC 1 cut(s) 321
SaqAI TTAA 4 cut(s) 29, 255, 857, 1044
Sau3AI GATC 8 cut(s) 125, 240, 572, 604, 881, 1188, 1349, 1357
Sau96I GGNCC 2 cut(s) 170, 453
SchI GAGTC 2 cut(s) 680, 1178
ScrFI CCNGG 1 cut(s) 1346
SduI GDGCHC 1 cut(s) 231
SfaNI GCATC 7 cut(s) 118, 743, 811, 812, 979, 1000, 1099
SfcI CTRYAG 2 cut(s) 586, 1005
SinI GGWCC 1 cut(s) 453
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 9 cut(s) 159, 354, 463, 484, 491, 547, 562, 970, 1232
SsiI CCGC 3 cut(s) 5, 107, 992
SspMI CTAG 3 cut(s) 885, 893, 1409
StyD4I CCNGG 1 cut(s) 1344
StyI CCWWGG 2 cut(s) 840, 961
TaaI ACNGT 2 cut(s) 235, 450
TaiI ACGT 1 cut(s) 543
TaqI TCGA 6 cut(s) 124, 213, 239, 1187, 1269, 1364
TaqII GACCGA 1 cut(s) 172
TasI AATT 9 cut(s) 159, 354, 463, 484, 491, 547, 562, 970, 1232
TfiI GAWTC 3 cut(s) 700, 1300, 1405
Tru1I TTAA 4 cut(s) 29, 255, 857, 1044
Tru9I TTAA 4 cut(s) 29, 255, 857, 1044
TscAI CASTG 1 cut(s) 1141
TspDTI ATGAA 4 cut(s) 321, 356, 1325, 1337
TspGWI ACGGA 2 cut(s) 77, 1350
TspRI CASTG 1 cut(s) 1141
Vha464I CTTAAG 1 cut(s) 254
VpaK11BI GGWCC 1 cut(s) 453
XapI RAATTY 6 cut(s) 463, 484, 491, 547, 562, 970
XbaI TCTAGA 1 cut(s) 884
XceI RCATGY 1 cut(s) 375
XspI CTAG 3 cut(s) 885, 893, 1409
Zsp2I ATGCAT 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.