Rroxscaffold_3G00260980

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0006747
Physical Location & Seq
Forward (+)
55389519 .. 55394427
4909 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00260980.1

Sequence Viewer

Length: 1269 bp
ATGGACCCTGCGTTCGCGGATGAGGGCCACGACGGTGAGACCGTGACAATCGAAGAGTATCTGAAGGATGTTGAGGACCAGGAACTGGCTTTATTAGTTTCCCTCGTCCTCTCCTTTAGTTTAGAGGAAAATATATTTTGTGACCGGGGACAAGAGTGTACCTATAATAATGGGTACATGAAGAGGCAAGCAATTTTCTCATGCTTGACTTGCACACCAGATGGTAATGCTGGAGTTTGCACAGCTTGCAGCTTGTCTTGTCATGATGGCCATGAGATTGTGGAACTATGGACCAAGAGAAACTTCAGATGTGATTGTGGAAATTCAAAATTTGGTGAGTTCTACTGCAAGATTTTCCCAAGTAAAGATATCGAAAATGTTGAGAACTCCTACAATCATAACTTCAAAGGTTCCTACTGTACCTGTGGCCGGCCCTATCCTGATCCAGACGTGGAAGAACAAGTAGAGATGATACAGTGCTGCTTCTGTGAGGACTGGTTCCATGAGGAACATATTGGTCTTGATTCCTCTGACGAGATTCCAAGAGATGGAGAAGGTGAACCTCTATATGAGGATTTTATATGTGCAGCATGCTCAAAAGTTTGCTCCTTTCTAAGACTATATACTCGAATCATCTTGGAGGCAAGGAGCCAAAACAATTCTTCTGTTAAGACTAGAAAAGATAAAGAAGTTATAGCAGATGAAGTACCTTTAGCTTGTGGATCTGGAAAGCTAGAAAATATTCCTCAGAGTTCTACTACCAACTCTGAATCTGTGTCTGGAGGGCAAGTTTCACTTGGAGAAAATTCTGGGAAGAATATATGTTCAGAGCAATGTGCTACAGATGCTGATACACGTCCTACTTGCGCTATTGGAGTTGATCTGGCAGTTGCTTCCCCTGTTTCAGAGAGTAAGCCAATGTTTCTTTCAAAAAACTGGCGGAATGCTCTTTGCAGGTGTGAAAAGTGCAGTGAATTTTATAAACAGAAGCATATTAGTTTTCTAATTGACAAGGAGGACTCAATCATTGAGTATGAGAAAATGGCAAAGCAGAAGAGAGAAGAAAAGTTGCAGCAACGTGAGGGGGCAGAGTTAAGTATGCTGGATAACCTTGGCCATGTAGAGAAAATTGAGATTCTGAATGGTATTGCAGATATCAAAGATGAGCTTTGTTCTTTCCTGGGATCATTTGATCCATCGAAAGCAATCACATCTGCTGATGTCCACCAGATTTTTGAGAATCTAAAGAAACGCAGGCGTGTAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-UBR

Protein Analysis

422

Amino Acids

47.43

Weight (kDa)

4.76

Isoelectric Point (pI)

55.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-UBR PF02207 53 - 117 2.6e-13 Putative zinc finger in N-recognin (UBR box)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 981
AarI CACCTGC 1 cut(s) 945
Acc36I ACCTGC 1 cut(s) 945
AccB7I CCANNNNNTGG 2 cut(s) 85, 548
AccII CGCG 1 cut(s) 17
AciI CCGC 2 cut(s) 17, 940
AclWI GGATC 4 cut(s) 437, 730, 1187, 1192
AcoI YGGCCR 3 cut(s) 268, 427, 1114
AcsI RAATTY 4 cut(s) 322, 329, 805, 974
AcuI CTGAAG 2 cut(s) 83, 289
AfaI GTAC 4 cut(s) 160, 176, 421, 708
AfiI CCNNNNNNNGG 3 cut(s) 85, 429, 548
AflIII ACRYGT 1 cut(s) 854
AgsI TTSAA 3 cut(s) 327, 406, 930
AjiI CACGTC 2 cut(s) 451, 857
AjnI CCWGG 2 cut(s) 78, 1179
AjuI GAANNNNNNNTTGG 4 cut(s) 287, 319, 910, 942
AleI CACNNNNGTG 1 cut(s) 33
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 5 cut(s) 245, 252, 716, 733, 1168
AluI AGCT 5 cut(s) 245, 252, 716, 733, 1168
Alw26I GTCTC 1 cut(s) 32
AlwI GGATC 4 cut(s) 437, 730, 1187, 1192
AlwNI CAGNNNCTG 2 cut(s) 85, 848
AoxI GGCC 5 cut(s) 25, 268, 427, 431, 1114
ApeKI GCWGC 4 cut(s) 249, 480, 587, 1072
ApoI RAATTY 4 cut(s) 322, 329, 805, 974
ArsI GACNNNNNNTTYG 1 cut(s) 28
Asp700I GAANNNNTTC 1 cut(s) 741
AspLEI GCGC 1 cut(s) 869
AspS9I GGNCC 5 cut(s) 4, 25, 76, 291, 432
AsuC2I CCSGG 1 cut(s) 146
AsuHPI GGTGA 3 cut(s) 47, 347, 569
AvaII GGWCC 3 cut(s) 4, 76, 291
BalI TGGCCA 2 cut(s) 270, 1116
BbvI GCAGC 4 cut(s) 261, 467, 599, 1084
BccI CCATC 4 cut(s) 215, 260, 542, 1204
BciT130I CCWGG 2 cut(s) 80, 1181
BcnI CCSGG 1 cut(s) 146
BcoDI GTCTC 1 cut(s) 32
BfaI CTAG 2 cut(s) 675, 734
BfmI CTRYAG 1 cut(s) 840
BfuAI ACCTGC 1 cut(s) 945
BisI GCNGC 4 cut(s) 250, 481, 588, 1073
BlsI GCNGC 4 cut(s) 251, 482, 589, 1074
Bme1390I CCNGG 3 cut(s) 80, 146, 1181
Bme18I GGWCC 3 cut(s) 4, 76, 291
BmgBI CACGTC 2 cut(s) 451, 857
BmgT120I GGNCC 5 cut(s) 4, 25, 76, 291, 432
BmiI GGNNCC 4 cut(s) 6, 412, 500, 650
BmrFI CCNGG 3 cut(s) 80, 146, 1181
BmsI GCATC 1 cut(s) 835
BpmI CTGGAG 2 cut(s) 252, 801
BpuMI CCSGG 1 cut(s) 146
BsaI GGTCTC 1 cut(s) 32
BsaJI CCNNGG 3 cut(s) 145, 1111, 1180
BsaXI ACNNNNNCTCC 2 cut(s) 774, 804
Bsc4I CCNNNNNNNGG 3 cut(s) 85, 429, 548
Bse118I RCCGGY 1 cut(s) 429
Bse1I ACTGG 3 cut(s) 90, 500, 941
Bse3DI GCAATG 1 cut(s) 839
BseBI CCWGG 2 cut(s) 80, 1181
BseDI CCNNGG 3 cut(s) 145, 1111, 1180
BseGI GGATG 2 cut(s) 25, 73
BseLI CCNNNNNNNGG 3 cut(s) 85, 429, 548
BseMI GCAATG 1 cut(s) 839
BseMII CTCAG 1 cut(s) 761
BseNI ACTGG 3 cut(s) 90, 500, 941
BseXI GCAGC 4 cut(s) 261, 467, 599, 1084
BsgI GTGCAG 2 cut(s) 606, 988
Bsh1236I CGCG 1 cut(s) 17
BshFI GGCC 5 cut(s) 27, 270, 429, 433, 1116
BsiSI CCGG 2 cut(s) 145, 430
BslFI GGGAC 1 cut(s) 162
BslI CCNNNNNNNGG 3 cut(s) 85, 429, 548
BsmAI GTCTC 1 cut(s) 32
BsmFI GGGAC 1 cut(s) 162
BsmI GAATGC 1 cut(s) 949
BsnI GGCC 5 cut(s) 27, 270, 429, 433, 1116
Bso31I GGTCTC 1 cut(s) 32
Bsp143I GATC 5 cut(s) 442, 722, 880, 1184, 1192
BspACI CCGC 2 cut(s) 17, 940
BspANI GGCC 5 cut(s) 27, 270, 429, 433, 1116
BspCNI CTCAG 1 cut(s) 760
BspFNI CGCG 1 cut(s) 17
BspHI TCATGA 1 cut(s) 262
BspLI GGNNCC 4 cut(s) 6, 412, 500, 650
BspMI ACCTGC 1 cut(s) 945
BspPI GGATC 4 cut(s) 437, 730, 1187, 1192
BspTNI GGTCTC 1 cut(s) 32
BsrDI GCAATG 1 cut(s) 839
BsrFI RCCGGY 1 cut(s) 429
BsrI ACTGG 3 cut(s) 90, 500, 941
BssAI RCCGGY 1 cut(s) 429
BssECI CCNNGG 3 cut(s) 145, 1111, 1180
BssMI GATC 5 cut(s) 442, 722, 880, 1184, 1192
BssT1I CCWWGG 1 cut(s) 1111
Bst2UI CCWGG 2 cut(s) 80, 1181
Bst4CI ACNGT 4 cut(s) 35, 43, 419, 477
Bst6I CTCTTC 3 cut(s) 48, 176, 1049
BstAPI GCANNNNNTGC 1 cut(s) 246
BstC8I GCNNGC 5 cut(s) 189, 247, 431, 592, 1256
BstDEI CTNAG 2 cut(s) 614, 747
BstF5I GGATG 2 cut(s) 25, 73
BstFNI CGCG 1 cut(s) 17
BstHHI GCGC 1 cut(s) 869
BstKTI GATC 5 cut(s) 445, 725, 883, 1187, 1195
BstMAI GTCTC 1 cut(s) 32
BstMBI GATC 5 cut(s) 442, 722, 880, 1184, 1192
BstMWI GCNNNNNNNGC 3 cut(s) 210, 246, 845
BstNI CCWGG 2 cut(s) 80, 1181
BstNSI RCATGY 1 cut(s) 594
BstSCI CCNGG 3 cut(s) 78, 144, 1179
BstSFI CTRYAG 1 cut(s) 840
BstUI CGCG 1 cut(s) 17
BstV1I GCAGC 4 cut(s) 261, 467, 599, 1084
BstX2I RGATCY 1 cut(s) 722
BstYI RGATCY 1 cut(s) 722
BsuRI GGCC 5 cut(s) 27, 270, 429, 433, 1116
BtrI CACGTC 2 cut(s) 451, 857
BtsCI GGATG 2 cut(s) 25, 73
BtsI GCAGTG 1 cut(s) 976
BtsIMutI CAGTG 2 cut(s) 482, 976
BveI ACCTGC 1 cut(s) 945
Cac8I GCNNGC 5 cut(s) 189, 247, 431, 592, 1256
CaiI CAGNNNCTG 2 cut(s) 85, 848
CciI TCATGA 1 cut(s) 262
CfoI GCGC 1 cut(s) 869
Cfr10I RCCGGY 1 cut(s) 429
Cfr13I GGNCC 5 cut(s) 4, 25, 76, 291, 432
Csp6I GTAC 4 cut(s) 159, 175, 420, 707
CviAII CATG 7 cut(s) 178, 201, 263, 272, 503, 591, 1118
CviQI GTAC 4 cut(s) 159, 175, 420, 707
DdeI CTNAG 2 cut(s) 614, 747
DpnI GATC 5 cut(s) 444, 724, 882, 1186, 1194
DpnII GATC 5 cut(s) 442, 722, 880, 1184, 1192
EaeI YGGCCR 3 cut(s) 268, 427, 1114
Eam1104I CTCTTC 3 cut(s) 48, 176, 1049
EarI CTCTTC 3 cut(s) 48, 176, 1049
EciI GGCGGA 1 cut(s) 955
Eco130I CCWWGG 1 cut(s) 1111
Eco31I GGTCTC 1 cut(s) 32
Eco32I GATATC 2 cut(s) 370, 1156
Eco47I GGWCC 3 cut(s) 4, 76, 291
Eco57I CTGAAG 2 cut(s) 83, 289
EcoRII CCWGG 2 cut(s) 78, 1179
EcoRV GATATC 2 cut(s) 370, 1156
EcoT14I CCWWGG 1 cut(s) 1111
ErhI CCWWGG 1 cut(s) 1111
FaeI CATG 7 cut(s) 181, 204, 266, 275, 506, 594, 1121
FalI AAGNNNNNCTT 6 cut(s) 287, 319, 780, 812, 1152, 1184
FaqI GGGAC 1 cut(s) 162
FatI CATG 7 cut(s) 177, 200, 262, 271, 502, 590, 1117
Fnu4HI GCNGC 4 cut(s) 250, 481, 588, 1073
FokI GGATG 2 cut(s) 32, 80
FseI GGCCGGCC 1 cut(s) 433
Fsp4HI GCNGC 4 cut(s) 250, 481, 588, 1073
FspBI CTAG 2 cut(s) 675, 734
GlaI GCGC 1 cut(s) 868
GluI GCNGC 4 cut(s) 250, 481, 588, 1073
GsuI CTGGAG 2 cut(s) 252, 801
HaeIII GGCC 5 cut(s) 27, 270, 429, 433, 1116
HapII CCGG 2 cut(s) 145, 430
HhaI GCGC 1 cut(s) 869
Hin1II CATG 7 cut(s) 181, 204, 266, 275, 506, 594, 1121
Hin6I GCGC 1 cut(s) 867
HinP1I GCGC 1 cut(s) 867
HinfI GANTC 7 cut(s) 524, 538, 630, 770, 1019, 1135, 1240
HpaII CCGG 2 cut(s) 145, 430
HphI GGTGA 3 cut(s) 47, 347, 569
Hpy166II GTNNAC 3 cut(s) 159, 560, 1225
Hpy188I TCNGA 8 cut(s) 63, 308, 532, 750, 769, 829, 907, 1140
Hpy188III TCNNGA 6 cut(s) 263, 440, 446, 521, 726, 780
Hpy8I GTNNAC 3 cut(s) 159, 560, 1225
Hpy99I CGWCG 1 cut(s) 35
HpyAV CCTTC 2 cut(s) 58, 548
HpyCH4III ACNGT 4 cut(s) 35, 43, 419, 477
HpyCH4IV ACGT 3 cut(s) 450, 856, 1078
HpyCH4V TGCA 9 cut(s) 213, 240, 249, 348, 587, 954, 969, 1072, 1151
HpyF10VI GCNNNNNNNGC 3 cut(s) 210, 246, 845
HpyF3I CTNAG 2 cut(s) 614, 747
HpySE526I ACGT 3 cut(s) 450, 856, 1078
Hsp92II CATG 7 cut(s) 181, 204, 266, 275, 506, 594, 1121
HspAI GCGC 1 cut(s) 867
KroI GCCGGC 1 cut(s) 429
KroNI GCCGGC 1 cut(s) 431
Kzo9I GATC 5 cut(s) 442, 722, 880, 1184, 1192
LmnI GCTCC 2 cut(s) 611, 648
Lsp1109I GCAGC 4 cut(s) 261, 467, 599, 1084
LweI GCATC 1 cut(s) 835
MaeI CTAG 2 cut(s) 675, 734
MaeII ACGT 3 cut(s) 450, 856, 1078
MaeIII GTNAC 2 cut(s) 43, 140
MalI GATC 5 cut(s) 444, 724, 882, 1186, 1194
MboI GATC 5 cut(s) 442, 722, 880, 1184, 1192
MboII GAAGA 7 cut(s) 65, 193, 467, 654, 826, 1066, 1073
MflI RGATCY 1 cut(s) 722
MlsI TGGCCA 2 cut(s) 270, 1116
MluCI AATT 8 cut(s) 192, 322, 329, 658, 805, 974, 1005, 1128
MluNI TGGCCA 2 cut(s) 270, 1116
MlyI GAGTC 1 cut(s) 1013
Mox20I TGGCCA 2 cut(s) 270, 1116
MroNI GCCGGC 1 cut(s) 429
MroXI GAANNNNTTC 1 cut(s) 741
MscI TGGCCA 2 cut(s) 270, 1116
MseI TTAA 2 cut(s) 669, 1094
MslI CAYNNNNRTG 1 cut(s) 33
Msp20I TGGCCA 2 cut(s) 270, 1116
MspI CCGG 2 cut(s) 145, 430
MspR9I CCNGG 3 cut(s) 80, 146, 1181
Mva1269I GAATGC 1 cut(s) 949
MvaI CCWGG 2 cut(s) 80, 1181
MvnI CGCG 1 cut(s) 17
MwoI GCNNNNNNNGC 3 cut(s) 210, 246, 845
NaeI GCCGGC 1 cut(s) 431
NciI CCSGG 1 cut(s) 146
NdeII GATC 5 cut(s) 442, 722, 880, 1184, 1192
NgoMIV GCCGGC 1 cut(s) 429
NlaIII CATG 7 cut(s) 181, 204, 266, 275, 506, 594, 1121
NlaIV GGNNCC 4 cut(s) 6, 412, 500, 650
NmuCI GTSAC 2 cut(s) 43, 140
NspI RCATGY 1 cut(s) 594
OliI CACNNNNGTG 1 cut(s) 33
PaeI GCATGC 1 cut(s) 594
PagI TCATGA 1 cut(s) 262
PaqCI CACCTGC 1 cut(s) 945
PcsI WCGNNNNNNNCGW 1 cut(s) 39
PctI GAATGC 1 cut(s) 949
PdiI GCCGGC 1 cut(s) 431
PdmI GAANNNNTTC 1 cut(s) 741
PfeI GAWTC 6 cut(s) 524, 538, 630, 770, 1135, 1240
PflMI CCANNNNNTGG 2 cut(s) 85, 548
PkrI GCNGC 4 cut(s) 251, 482, 589, 1074
PleI GAGTC 1 cut(s) 1013
PpsI GAGTC 1 cut(s) 1013
PsiI TTATAA 1 cut(s) 981
Psp6I CCWGG 2 cut(s) 78, 1179
PspGI CCWGG 2 cut(s) 78, 1179
PspN4I GGNNCC 4 cut(s) 6, 412, 500, 650
PspPI GGNCC 5 cut(s) 4, 25, 76, 291, 432
PstNI CAGNNNCTG 2 cut(s) 85, 848
PsuI RGATCY 1 cut(s) 722
RigI GGCCGGCC 1 cut(s) 433
RsaI GTAC 4 cut(s) 160, 176, 421, 708
RsaNI GTAC 4 cut(s) 159, 175, 420, 707
RseI CAYNNNNRTG 1 cut(s) 33
SaqAI TTAA 2 cut(s) 669, 1094
SatI GCNGC 4 cut(s) 250, 481, 588, 1073
Sau3AI GATC 5 cut(s) 442, 722, 880, 1184, 1192
Sau96I GGNCC 5 cut(s) 4, 25, 76, 291, 432
SchI GAGTC 1 cut(s) 1013
ScrFI CCNGG 3 cut(s) 80, 146, 1181
SfaNI GCATC 1 cut(s) 835
SfcI CTRYAG 1 cut(s) 840
SinI GGWCC 3 cut(s) 4, 76, 291
SmiMI CAYNNNNRTG 1 cut(s) 33
SphI GCATGC 1 cut(s) 594
Sse9I AATT 8 cut(s) 192, 322, 329, 658, 805, 974, 1005, 1128
SsiI CCGC 2 cut(s) 17, 940
SspI AATATT 1 cut(s) 742
SspMI CTAG 2 cut(s) 675, 734
StyD4I CCNGG 3 cut(s) 78, 144, 1179
StyI CCWWGG 1 cut(s) 1111
TaaI ACNGT 4 cut(s) 35, 43, 419, 477
TaiI ACGT 3 cut(s) 453, 859, 1081
TaqI TCGA 4 cut(s) 51, 372, 628, 1199
TasI AATT 8 cut(s) 192, 322, 329, 658, 805, 974, 1005, 1128
TfiI GAWTC 6 cut(s) 524, 538, 630, 770, 1135, 1240
Tru1I TTAA 2 cut(s) 669, 1094
Tru9I TTAA 2 cut(s) 669, 1094
TscAI CASTG 2 cut(s) 482, 976
TseFI GTSAC 2 cut(s) 43, 140
TseI GCWGC 4 cut(s) 249, 480, 587, 1072
Tsp45I GTSAC 2 cut(s) 43, 140
TspDTI ATGAA 2 cut(s) 194, 717
TspRI CASTG 2 cut(s) 482, 976
Van91I CCANNNNNTGG 2 cut(s) 85, 548
VpaK11BI GGWCC 3 cut(s) 4, 76, 291
XapI RAATTY 4 cut(s) 322, 329, 805, 974
XceI RCATGY 1 cut(s) 594
XmnI GAANNNNTTC 1 cut(s) 741
XspI CTAG 2 cut(s) 675, 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.