Rw4G009500

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0006747
Physical Location & Seq
Reverse (-)
21437790 .. 21440836
3047 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G009500.1

Sequence Viewer

Length: 306 bp
ATGTTGAGGACCAGGAACTGGAAGCTGATTTGGTTTTGGGGGGGGGGCGATGAAGGCAAGGAGTGTACCTATAATAATGAGTACATGAAGAGGCAAGCAATTTTCTCATGCTTGACTTGCACACCAGATGGTAATGCTGGAGATCATCATACCCGGCCACCATATTTTTCAGGCCACTTCTACTGGAGATCATCATACCCGGCCACCACTTTTCTTCAGACCACTTGTTTGGGTGATCGACGGGATGATTTGGCAGACGATTCGTATAATCGTGGTTGTGCTGCTAAGAAGACGCAGCTGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-UBR

Protein Analysis

101

Amino Acids

11.73

Weight (kDa)

7.64

Isoelectric Point (pI)

59.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 18
AcoI YGGCCR 2 cut(s) 155, 201
AcuI CTGAAG 1 cut(s) 200
AfaI GTAC 2 cut(s) 67, 83
AfiI CCNNNNNNNGG 1 cut(s) 18
AjnI CCWGG 1 cut(s) 11
AluBI AGCT 2 cut(s) 25, 298
AluI AGCT 2 cut(s) 25, 298
AlwNI CAGNNNCTG 1 cut(s) 18
AoxI GGCC 3 cut(s) 155, 172, 201
ApeKI GCWGC 2 cut(s) 281, 295
ArsI GACNNNNNNTTYG 2 cut(s) 211, 243
AspS9I GGNCC 1 cut(s) 9
AsuC2I CCSGG 2 cut(s) 154, 200
AsuHPI GGTGA 1 cut(s) 245
AvaII GGWCC 1 cut(s) 9
BbsI GAAGAC 1 cut(s) 296
BbvI GCAGC 1 cut(s) 268
BccI CCATC 1 cut(s) 122
BcgI CGANNNNNNTGC 2 cut(s) 243, 277
BciT130I CCWGG 1 cut(s) 13
BcnI CCSGG 2 cut(s) 154, 200
BisI GCNGC 2 cut(s) 282, 296
BlsI GCNGC 2 cut(s) 283, 297
Bme1390I CCNGG 3 cut(s) 13, 154, 200
Bme18I GGWCC 1 cut(s) 9
BmgT120I GGNCC 1 cut(s) 9
BmrFI CCNGG 3 cut(s) 13, 154, 200
BpiI GAAGAC 1 cut(s) 296
BpmI CTGGAG 2 cut(s) 159, 205
BpuMI CCSGG 2 cut(s) 154, 200
Bsc4I CCNNNNNNNGG 1 cut(s) 18
Bse1I ACTGG 2 cut(s) 23, 188
BseBI CCWGG 1 cut(s) 13
BseGI GGATG 1 cut(s) 250
BseLI CCNNNNNNNGG 1 cut(s) 18
BseNI ACTGG 2 cut(s) 23, 188
BseXI GCAGC 1 cut(s) 268
BshFI GGCC 3 cut(s) 157, 174, 203
BsiSI CCGG 2 cut(s) 154, 200
BslI CCNNNNNNNGG 1 cut(s) 18
BsnI GGCC 3 cut(s) 157, 174, 203
Bsp143I GATC 3 cut(s) 142, 188, 235
BspANI GGCC 3 cut(s) 157, 174, 203
BsrI ACTGG 2 cut(s) 23, 188
BssMI GATC 3 cut(s) 142, 188, 235
Bst2UI CCWGG 1 cut(s) 13
Bst6I CTCTTC 1 cut(s) 83
BstC8I GCNNGC 1 cut(s) 96
BstDEI CTNAG 1 cut(s) 285
BstF5I GGATG 1 cut(s) 250
BstKTI GATC 3 cut(s) 145, 191, 238
BstMBI GATC 3 cut(s) 142, 188, 235
BstMWI GCNNNNNNNGC 2 cut(s) 54, 117
BstNI CCWGG 1 cut(s) 13
BstSCI CCNGG 3 cut(s) 11, 152, 198
BstV1I GCAGC 1 cut(s) 268
BstV2I GAAGAC 1 cut(s) 296
BstXI CCANNNNNNTGG 1 cut(s) 229
BsuRI GGCC 3 cut(s) 157, 174, 203
BtgZI GCGATG 1 cut(s) 63
BtsCI GGATG 1 cut(s) 250
Cac8I GCNNGC 1 cut(s) 96
CaiI CAGNNNCTG 1 cut(s) 18
Cfr13I GGNCC 1 cut(s) 9
CseI GACGC 1 cut(s) 301
Csp6I GTAC 2 cut(s) 66, 82
CviAII CATG 2 cut(s) 85, 108
CviJI RGCY 5 cut(s) 25, 157, 174, 203, 298
CviKI_1 RGCY 5 cut(s) 25, 157, 174, 203, 298
CviQI GTAC 2 cut(s) 66, 82
DdeI CTNAG 1 cut(s) 285
DpnI GATC 3 cut(s) 144, 190, 237
DpnII GATC 3 cut(s) 142, 188, 235
EaeI YGGCCR 2 cut(s) 155, 201
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
Eco47I GGWCC 1 cut(s) 9
Eco57I CTGAAG 1 cut(s) 200
EcoRII CCWGG 1 cut(s) 11
FaeI CATG 2 cut(s) 88, 111
FaiI YATR 7 cut(s) 72, 86, 109, 150, 163, 196, 267
FatI CATG 2 cut(s) 84, 107
Fnu4HI GCNGC 2 cut(s) 282, 296
FokI GGATG 1 cut(s) 257
Fsp4HI GCNGC 2 cut(s) 282, 296
GluI GCNGC 2 cut(s) 282, 296
GsuI CTGGAG 2 cut(s) 159, 205
HaeIII GGCC 3 cut(s) 157, 174, 203
HapII CCGG 2 cut(s) 154, 200
HgaI GACGC 1 cut(s) 301
Hin1II CATG 2 cut(s) 88, 111
HinfI GANTC 1 cut(s) 260
HpaII CCGG 2 cut(s) 154, 200
HphI GGTGA 1 cut(s) 245
Hpy166II GTNNAC 1 cut(s) 66
Hpy188I TCNGA 1 cut(s) 219
Hpy8I GTNNAC 1 cut(s) 66
Hpy99I CGWCG 1 cut(s) 243
HpyAV CCTTC 1 cut(s) 47
HpyCH4V TGCA 1 cut(s) 120
HpyF10VI GCNNNNNNNGC 2 cut(s) 54, 117
HpyF3I CTNAG 1 cut(s) 285
Hsp92II CATG 2 cut(s) 88, 111
Kzo9I GATC 3 cut(s) 142, 188, 235
LpnPI CCDG 9 cut(s) 4, 25, 123, 138, 156, 167, 169, 213, 284
Lsp1109I GCAGC 1 cut(s) 268
MalI GATC 3 cut(s) 144, 190, 237
MboI GATC 3 cut(s) 142, 188, 235
MboII GAAGA 3 cut(s) 100, 206, 301
MluCI AATT 1 cut(s) 99
MnlI CCTC 1 cut(s) 84
MspA1I CMGCKG 1 cut(s) 298
MspI CCGG 2 cut(s) 154, 200
MspR9I CCNGG 3 cut(s) 13, 154, 200
MvaI CCWGG 1 cut(s) 13
MwoI GCNNNNNNNGC 2 cut(s) 54, 117
NciI CCSGG 2 cut(s) 154, 200
NdeII GATC 3 cut(s) 142, 188, 235
NlaIII CATG 2 cut(s) 88, 111
PfeI GAWTC 1 cut(s) 260
PflMI CCANNNNNTGG 1 cut(s) 18
PkrI GCNGC 2 cut(s) 283, 297
Psp6I CCWGG 1 cut(s) 11
PspGI CCWGG 1 cut(s) 11
PspPI GGNCC 1 cut(s) 9
PstNI CAGNNNCTG 1 cut(s) 18
PvuII CAGCTG 1 cut(s) 298
RsaI GTAC 2 cut(s) 67, 83
RsaNI GTAC 2 cut(s) 66, 82
SatI GCNGC 2 cut(s) 282, 296
Sau3AI GATC 3 cut(s) 142, 188, 235
Sau96I GGNCC 1 cut(s) 9
ScrFI CCNGG 3 cut(s) 13, 154, 200
SetI ASST 3 cut(s) 27, 71, 300
SinI GGWCC 1 cut(s) 9
Sse9I AATT 1 cut(s) 99
StyD4I CCNGG 3 cut(s) 11, 152, 198
TaqI TCGA 1 cut(s) 238
TasI AATT 1 cut(s) 99
TatI WGTACW 1 cut(s) 81
TfiI GAWTC 1 cut(s) 260
TseI GCWGC 2 cut(s) 281, 295
TspDTI ATGAA 2 cut(s) 66, 101
Van91I CCANNNNNTGG 1 cut(s) 18
VpaK11BI GGWCC 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.