MD14G1128900.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006747
Physical Location & Seq
Forward (+)
20604945 .. 20609182
4238 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1128900.v1.1.491

Sequence Viewer

Length: 1239 bp
ATGGACAGTGCATTCGAGGATGAGGCCGAGCACACCGTTTCGATCCAAGAGTATCTTAAGGATGTTGAGGAACAGGAACTGGAAGCTGATTTGGTTTTGGGAGGCGACGAAGGCAAGGAGTGTACCTATAACAATGGTTATATGAAGAGGCAGGCAATTTTCTCATGCCTGACTTGCACCCCAGACGGGAATGCTGGAGTCTGCACAGCTTGCAGCTTGTCTTGCCATGATGGCCATGAGATTGTAGAACTGTGGACCAAAAGAAATTTTAGATGTGATTGTGGTAATTCAAAATTTGGAGAGTTCTTTTGCAAGCTTTCCCCAAATAAAGATATAGAAAATGTTGAAAATTCTTACAATCAGAATTTCAAAGGATCATACTGCACCTGCAGTCGCCCCTATCCTGATCCAGATGTTGAAGAACAAGTAGAGATGATACAATGCTGTGTGTGTGAAGACTGGTTCCATGAGGAACACATCGGTCTTGACTCTTCTGATGAGATTCCAAGAGATGAGGAAGGTGAACCTCTTTATGAGGATTTTATTTGTAAGGGGTGCTCAGCAGTTTGCTCTTTTATAAGACTCTACAATCAAACCATCTTGGCATCTGGGAGCCAAAAAGATGCTCCCGTTGAGACTGCCAAGGATAAAAAGGTTTTGAGCGATATGCCTTTAGCTTGTAGATCTGGAAAGCTAGAGAATGATATTTCTCAAAGTTCTGATACTATTTCTGAACCTGTATCTGGTGGGGATAGCTTGCTCCTTGGAGAAAATTCTGGGAAGAATACAGATTCGGATCAATGCATTACAGATGCCAATGCACCTGTTACTTGTGTTATTGGAGTTAATCTGGTGGTTGCTTCCCCTGTTTTAGAGTGTAAGCCAATGTTTCTTCTGAAAAACTGGAGGGATGCACTCTGCAAGTGTGAAAAATGCAGTGAATTTTACGAACAGAAGCATATTAGGTTTCTGCTTGACAAGGAAGACTCGATCATGGAGTATGAGAAAATGGCAAAGCAAAAGAGAGAGGAAAAATTGCAGCAACAGGAGGGGGCTGAGTTGACTATGCTCGATAAACTTGGCCATGTCGAGAAAATAGAGATTCTGAGTGGCATCGCAGACATGAAAGATGAGCTTCGTTCTTTCCTGGGATCATTTGATCCATCAAAGACAATCACCTCTGCTGATGTCCACCAGATTTTTGAGAATCTAGCAAAGAAACGCAGGCGTGTAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-UBR

Protein Analysis

413

Amino Acids

46.34

Weight (kDa)

4.61

Isoelectric Point (pI)

55.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-UBR PF02207 41 - 106 1.2e-13 Putative zinc finger in N-recognin (UBR box)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 578
AarI CACCTGC 1 cut(s) 395
Acc36I ACCTGC 1 cut(s) 395
AclWI GGATC 6 cut(s) 37, 382, 401, 804, 1154, 1159
AcoI YGGCCR 2 cut(s) 232, 1081
AcsI RAATTY 6 cut(s) 265, 293, 349, 364, 772, 941
AfaI GTAC 1 cut(s) 124
AfiI CCNNNNNNNGG 2 cut(s) 186, 743
AflII CTTAAG 1 cut(s) 56
AgsI TTSAA 4 cut(s) 291, 347, 370, 419
AjnI CCWGG 1 cut(s) 1146
AluBI AGCT 8 cut(s) 86, 209, 216, 316, 677, 694, 756, 1135
AluI AGCT 8 cut(s) 86, 209, 216, 316, 677, 694, 756, 1135
Alw21I GWGCWC 2 cut(s) 33, 560
Alw26I GTCTC 1 cut(s) 629
AlwI GGATC 6 cut(s) 37, 382, 401, 804, 1154, 1159
AlwNI CAGNNNCTG 1 cut(s) 79
AoxI GGCC 3 cut(s) 24, 232, 1081
ApeKI GCWGC 2 cut(s) 213, 1039
ApoI RAATTY 6 cut(s) 265, 293, 349, 364, 772, 941
ArsI GACNNNNNNTTYG 1 cut(s) 28
AspS9I GGNCC 1 cut(s) 255
AsuHPI GGTGA 2 cut(s) 533, 1168
AvaII GGWCC 1 cut(s) 255
BalI TGGCCA 2 cut(s) 234, 1083
BbsI GAAGAC 2 cut(s) 462, 990
Bbv12I GWGCWC 2 cut(s) 33, 560
BbvI GCAGC 2 cut(s) 225, 1051
BccI CCATC 3 cut(s) 224, 605, 1171
BciT130I CCWGG 1 cut(s) 1148
BcoDI GTCTC 1 cut(s) 629
BfaI CTAG 2 cut(s) 695, 1211
BfmI CTRYAG 1 cut(s) 388
BfrI CTTAAG 1 cut(s) 56
BfuAI ACCTGC 1 cut(s) 395
BglI GCCNNNNNGGC 1 cut(s) 231
BglII AGATCT 1 cut(s) 683
BisI GCNGC 2 cut(s) 214, 1040
BlpI GCTNAGC 1 cut(s) 559
BlsI GCNGC 2 cut(s) 215, 1041
Bme1390I CCNGG 1 cut(s) 1148
Bme18I GGWCC 1 cut(s) 255
BmgT120I GGNCC 1 cut(s) 255
BmiI GGNNCC 2 cut(s) 464, 614
BmrFI CCNGG 1 cut(s) 1148
BmsI GCATC 5 cut(s) 613, 614, 802, 901, 1122
BpiI GAAGAC 2 cut(s) 462, 990
BpmI CTGGAG 2 cut(s) 216, 925
Bpu1102I GCTNAGC 1 cut(s) 559
BsaBI GATNNNNATC 1 cut(s) 795
BsaJI CCNNGG 3 cut(s) 642, 763, 1147
Bsc4I CCNNNNNNNGG 2 cut(s) 186, 743
Bse1I ACTGG 3 cut(s) 84, 464, 908
Bse8I GATNNNNATC 1 cut(s) 795
BseBI CCWGG 1 cut(s) 1148
BseDI CCNNGG 3 cut(s) 642, 763, 1147
BseGI GGATG 3 cut(s) 25, 67, 916
BseJI GATNNNNATC 1 cut(s) 795
BseLI CCNNNNNNNGG 2 cut(s) 186, 743
BseMII CTCAG 3 cut(s) 573, 1047, 1097
BseNI ACTGG 3 cut(s) 84, 464, 908
BseXI GCAGC 2 cut(s) 225, 1051
BsgI GTGCAG 2 cut(s) 187, 367
BshFI GGCC 3 cut(s) 26, 234, 1083
BsiHKAI GWGCWC 2 cut(s) 33, 560
BslI CCNNNNNNNGG 2 cut(s) 186, 743
BsmAI GTCTC 1 cut(s) 629
BsmI GAATGC 2 cut(s) 11, 196
BsnI GGCC 3 cut(s) 26, 234, 1083
Bsp1286I GDGCHC 2 cut(s) 33, 560
Bsp143I GATC 8 cut(s) 42, 374, 406, 683, 796, 990, 1151, 1159
Bsp1720I GCTNAGC 1 cut(s) 559
BspANI GGCC 3 cut(s) 26, 234, 1083
BspCNI CTCAG 3 cut(s) 572, 1048, 1098
BspLI GGNNCC 2 cut(s) 464, 614
BspMAI CTGCAG 1 cut(s) 392
BspMI ACCTGC 1 cut(s) 395
BspPI GGATC 6 cut(s) 37, 382, 401, 804, 1154, 1159
BspTI CTTAAG 1 cut(s) 56
BsrI ACTGG 3 cut(s) 84, 464, 908
BssECI CCNNGG 3 cut(s) 642, 763, 1147
BssMI GATC 8 cut(s) 42, 374, 406, 683, 796, 990, 1151, 1159
BssT1I CCWWGG 2 cut(s) 642, 763
Bst2UI CCWGG 1 cut(s) 1148
Bst4CI ACNGT 3 cut(s) 8, 37, 252
Bst6I CTCTTC 2 cut(s) 140, 496
BstAFI CTTAAG 1 cut(s) 56
BstAPI GCANNNNNTGC 1 cut(s) 210
BstC8I GCNNGC 5 cut(s) 153, 211, 314, 758, 1226
BstDEI CTNAG 3 cut(s) 559, 1056, 1106
BstF5I GGATG 3 cut(s) 25, 67, 916
BstKTI GATC 8 cut(s) 45, 377, 409, 686, 799, 993, 1154, 1162
BstMAI GTCTC 1 cut(s) 629
BstMBI GATC 8 cut(s) 42, 374, 406, 683, 796, 990, 1151, 1159
BstMWI GCNNNNNNNGC 5 cut(s) 111, 174, 210, 222, 231
BstNI CCWGG 1 cut(s) 1148
BstSCI CCNGG 1 cut(s) 1146
BstSFI CTRYAG 1 cut(s) 388
BstV1I GCAGC 2 cut(s) 225, 1051
BstV2I GAAGAC 2 cut(s) 462, 990
BstX2I RGATCY 1 cut(s) 683
BstYI RGATCY 1 cut(s) 683
BsuRI GGCC 3 cut(s) 26, 234, 1083
BtgZI GCGATG 1 cut(s) 1099
BtsCI GGATG 3 cut(s) 25, 67, 916
BtsI GCAGTG 1 cut(s) 943
BtsIMutI CAGTG 2 cut(s) 13, 943
BveI ACCTGC 1 cut(s) 395
Cac8I GCNNGC 5 cut(s) 153, 211, 314, 758, 1226
CaiI CAGNNNCTG 1 cut(s) 79
Cfr13I GGNCC 1 cut(s) 255
Csp6I GTAC 1 cut(s) 123
CviAII CATG 7 cut(s) 165, 227, 236, 467, 994, 1085, 1123
CviQI GTAC 1 cut(s) 123
DdeI CTNAG 3 cut(s) 559, 1056, 1106
DpnI GATC 8 cut(s) 44, 376, 408, 685, 798, 992, 1153, 1161
DpnII GATC 8 cut(s) 42, 374, 406, 683, 796, 990, 1151, 1159
EaeI YGGCCR 2 cut(s) 232, 1081
Eam1104I CTCTTC 2 cut(s) 140, 496
EarI CTCTTC 2 cut(s) 140, 496
Eco130I CCWWGG 2 cut(s) 642, 763
Eco47I GGWCC 1 cut(s) 255
EcoRII CCWGG 1 cut(s) 1146
EcoT14I CCWWGG 2 cut(s) 642, 763
EcoT22I ATGCAT 1 cut(s) 806
ErhI CCWWGG 2 cut(s) 642, 763
FaeI CATG 7 cut(s) 168, 230, 239, 470, 997, 1088, 1126
FalI AAGNNNNNCTT 4 cut(s) 39, 71, 1119, 1151
FatI CATG 7 cut(s) 164, 226, 235, 466, 993, 1084, 1122
Fnu4HI GCNGC 2 cut(s) 214, 1040
FokI GGATG 3 cut(s) 32, 74, 923
Fsp4HI GCNGC 2 cut(s) 214, 1040
FspBI CTAG 2 cut(s) 695, 1211
GluI GCNGC 2 cut(s) 214, 1040
GsuI CTGGAG 2 cut(s) 216, 925
HaeIII GGCC 3 cut(s) 26, 234, 1083
Hin1II CATG 7 cut(s) 168, 230, 239, 470, 997, 1088, 1126
HincII GTYRAC 1 cut(s) 1062
HindII GTYRAC 1 cut(s) 1062
HindIII AAGCTT 1 cut(s) 314
HinfI GANTC 8 cut(s) 198, 488, 502, 582, 791, 986, 1102, 1207
HphI GGTGA 2 cut(s) 533, 1168
Hpy166II GTNNAC 5 cut(s) 123, 255, 524, 1062, 1192
Hpy188I TCNGA 7 cut(s) 363, 496, 721, 733, 796, 897, 1107
Hpy188III TCNNGA 5 cut(s) 404, 410, 485, 687, 1090
Hpy8I GTNNAC 5 cut(s) 123, 255, 524, 1062, 1192
Hpy99I CGWCG 1 cut(s) 110
HpyAV CCTTC 2 cut(s) 104, 512
HpyCH4III ACNGT 3 cut(s) 8, 37, 252
HpyF10VI GCNNNNNNNGC 5 cut(s) 111, 174, 210, 222, 231
HpyF3I CTNAG 3 cut(s) 559, 1056, 1106
Hsp92II CATG 7 cut(s) 168, 230, 239, 470, 997, 1088, 1126
Kzo9I GATC 8 cut(s) 42, 374, 406, 683, 796, 990, 1151, 1159
LmnI GCTCC 3 cut(s) 612, 631, 765
Lsp1109I GCAGC 2 cut(s) 225, 1051
LweI GCATC 5 cut(s) 613, 614, 802, 901, 1122
MaeI CTAG 2 cut(s) 695, 1211
MaeIII GTNAC 1 cut(s) 826
MalI GATC 8 cut(s) 44, 376, 408, 685, 798, 992, 1153, 1161
MboI GATC 8 cut(s) 42, 374, 406, 683, 796, 990, 1151, 1159
MboII GAAGA 7 cut(s) 157, 431, 467, 483, 793, 884, 995
MflI RGATCY 1 cut(s) 683
MhlI GDGCHC 2 cut(s) 33, 560
MlsI TGGCCA 2 cut(s) 234, 1083
MluCI AATT 9 cut(s) 156, 265, 286, 293, 349, 364, 772, 941, 1034
MluNI TGGCCA 2 cut(s) 234, 1083
MlyI GAGTC 4 cut(s) 207, 482, 576, 980
Mox20I TGGCCA 2 cut(s) 234, 1083
Mph1103I ATGCAT 1 cut(s) 806
MscI TGGCCA 2 cut(s) 234, 1083
MseI TTAA 2 cut(s) 57, 846
Msp20I TGGCCA 2 cut(s) 234, 1083
MspCI CTTAAG 1 cut(s) 56
MspR9I CCNGG 1 cut(s) 1148
Mva1269I GAATGC 2 cut(s) 11, 196
MvaI CCWGG 1 cut(s) 1148
MwoI GCNNNNNNNGC 5 cut(s) 111, 174, 210, 222, 231
NdeII GATC 8 cut(s) 42, 374, 406, 683, 796, 990, 1151, 1159
NlaIII CATG 7 cut(s) 168, 230, 239, 470, 997, 1088, 1126
NlaIV GGNNCC 2 cut(s) 464, 614
NmeAIII GCCGAG 1 cut(s) 52
NsiI ATGCAT 1 cut(s) 806
PaqCI CACCTGC 1 cut(s) 395
PctI GAATGC 2 cut(s) 11, 196
PfeI GAWTC 4 cut(s) 502, 791, 1102, 1207
PkrI GCNGC 2 cut(s) 215, 1041
PleI GAGTC 4 cut(s) 206, 482, 576, 980
PpsI GAGTC 4 cut(s) 206, 482, 576, 980
PsiI TTATAA 1 cut(s) 578
Psp6I CCWGG 1 cut(s) 1146
PspGI CCWGG 1 cut(s) 1146
PspN4I GGNNCC 2 cut(s) 464, 614
PspPI GGNCC 1 cut(s) 255
PstI CTGCAG 1 cut(s) 392
PstNI CAGNNNCTG 1 cut(s) 79
PsuI RGATCY 1 cut(s) 683
RsaI GTAC 1 cut(s) 124
RsaNI GTAC 1 cut(s) 123
SaqAI TTAA 2 cut(s) 57, 846
SatI GCNGC 2 cut(s) 214, 1040
Sau3AI GATC 8 cut(s) 42, 374, 406, 683, 796, 990, 1151, 1159
Sau96I GGNCC 1 cut(s) 255
SchI GAGTC 4 cut(s) 207, 482, 576, 980
ScrFI CCNGG 1 cut(s) 1148
SduI GDGCHC 2 cut(s) 33, 560
SfaNI GCATC 5 cut(s) 613, 614, 802, 901, 1122
SfcI CTRYAG 1 cut(s) 388
SinI GGWCC 1 cut(s) 255
SmlI CTYRAG 1 cut(s) 56
SmoI CTYRAG 1 cut(s) 56
Sse9I AATT 9 cut(s) 156, 265, 286, 293, 349, 364, 772, 941, 1034
SspMI CTAG 2 cut(s) 695, 1211
StyD4I CCNGG 1 cut(s) 1146
StyI CCWWGG 2 cut(s) 642, 763
TaaI ACNGT 3 cut(s) 8, 37, 252
TaqI TCGA 5 cut(s) 15, 41, 989, 1071, 1089
TaqII GACCGA 1 cut(s) 470
TasI AATT 9 cut(s) 156, 265, 286, 293, 349, 364, 772, 941, 1034
TfiI GAWTC 4 cut(s) 502, 791, 1102, 1207
Tru1I TTAA 2 cut(s) 57, 846
Tru9I TTAA 2 cut(s) 57, 846
TscAI CASTG 2 cut(s) 13, 943
TseI GCWGC 2 cut(s) 213, 1039
TspDTI ATGAA 2 cut(s) 158, 1139
TspRI CASTG 2 cut(s) 13, 943
Vha464I CTTAAG 1 cut(s) 56
VpaK11BI GGWCC 1 cut(s) 255
XapI RAATTY 6 cut(s) 265, 293, 349, 364, 772, 941
XspI CTAG 2 cut(s) 695, 1211
Zsp2I ATGCAT 1 cut(s) 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.