FvH4_5g04950

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0010034
Physical Location & Seq
Reverse (-)
2933734 .. 2934375
642 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g04950.t1

Sequence Viewer

Length: 642 bp
ATGGGAAATTGCTCTTCTCTCAAAGGAACCTCTGGGGAGTGTCCTAACTCTATTAGGGTCCTTACAGATTCTGGGGGTATCCTACAGTTTAAAGGTCCTAAGTTAGCTTCTGAGATCCTAGCTGGTTTTCCAGGCTATGGCCTTTTTCAGCAGGGCAAGGCCTCGGTGCCATTGCCTGAGGAAGAGCCCTTAACTAGTGGTCAGTTCTACCTTCTACCACTGAGGCCTAAAGAGGAATTGCCTGCTGACAATGGGGACTCTGTACAAGTTCAAGAAGTGAGTGTTGAGGAAGATTTGGAGGGTGCCGAAGAAGCTGAGCCGGTGAAGAAGTCATTCGGGGGTGCTTCAGAATATGTGGAGAATTTGGCGAGTGTGTCAGGAGGAGGTCTGGAAGTCTTGCCGTGCGGCGGAGATGGGGTTTGGAGAGTGAAGCTTCTGATTGACACTAAGCAGTTGGAGGAGATTTTGTCTGAAGGCAACACTCTGGCTCTGATAGAGATGATGAGGATGGCTGCAACTACACCAAAGCTGCAGAGTAAGAGCCTTTGGGGTTCAGGAGGAGGAGGGGGTTGGAGTTGGAAACCTATCTTCTCCAGCATGTTTAAGGCCCCTATGGCCATAACTGATCGCAGTGGGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

214

Amino Acids

22.54

Weight (kDa)

4.62

Isoelectric Point (pI)

54.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 195 5.2e-13 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 166, 302
AccB7I CCANNNNNTGG 1 cut(s) 137
AciI CCGC 2 cut(s) 405, 408
AclWI GGATC 1 cut(s) 109
AcoI YGGCCR 1 cut(s) 615
AcsI RAATTY 1 cut(s) 361
AcuI CTGAAG 2 cut(s) 330, 492
AfaI GTAC 1 cut(s) 264
AfiI CCNNNNNNNGG 2 cut(s) 137, 407
AgsI TTSAA 1 cut(s) 272
AhlI ACTAGT 1 cut(s) 194
AjnI CCWGG 1 cut(s) 130
AluBI AGCT 5 cut(s) 107, 122, 314, 433, 529
AluI AGCT 5 cut(s) 107, 122, 314, 433, 529
AlwI GGATC 1 cut(s) 109
AlwNI CAGNNNCTG 1 cut(s) 71
AoxI GGCC 5 cut(s) 139, 159, 224, 606, 615
ApeKI GCWGC 2 cut(s) 512, 529
ApoI RAATTY 1 cut(s) 361
Asp700I GAANNNNTTC 1 cut(s) 332
AspS9I GGNCC 3 cut(s) 58, 95, 607
AsuHPI GGTGA 1 cut(s) 334
AvaII GGWCC 2 cut(s) 58, 95
AxyI CCTNAGG 1 cut(s) 177
BalI TGGCCA 1 cut(s) 617
BanI GGYRCC 2 cut(s) 166, 302
BanII GRGCYC 1 cut(s) 189
BbvI GCAGC 2 cut(s) 499, 516
BccI CCATC 2 cut(s) 407, 502
BceAI ACGGC 1 cut(s) 385
BciT130I CCWGG 1 cut(s) 132
BciVI GTATCC 1 cut(s) 89
BcuI ACTAGT 1 cut(s) 194
BfaI CTAG 2 cut(s) 119, 195
BfmI CTRYAG 2 cut(s) 83, 530
BfuI GTATCC 1 cut(s) 89
BglI GCCNNNNNGGC 1 cut(s) 614
BisI GCNGC 3 cut(s) 406, 513, 530
BlpI GCTNAGC 1 cut(s) 315
BlsI GCNGC 3 cut(s) 407, 514, 531
Bme1390I CCNGG 1 cut(s) 132
Bme18I GGWCC 2 cut(s) 58, 95
BmgT120I GGNCC 3 cut(s) 58, 95, 607
BmiI GGNNCC 5 cut(s) 28, 59, 168, 304, 609
BmrFI CCNGG 1 cut(s) 132
BpmI CTGGAG 1 cut(s) 577
Bpu1102I GCTNAGC 1 cut(s) 315
BsaJI CCNNGG 1 cut(s) 162
BsaXI ACNNNNNCTCC 4 cut(s) 402, 432, 452, 482
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 407
Bse118I RCCGGY 1 cut(s) 319
Bse21I CCTNAGG 1 cut(s) 177
Bse3DI GCAATG 1 cut(s) 170
BseBI CCWGG 1 cut(s) 132
BseDI CCNNGG 1 cut(s) 162
BseGI GGATG 2 cut(s) 513, 642
BseLI CCNNNNNNNGG 2 cut(s) 137, 407
BseMI GCAATG 1 cut(s) 170
BseMII CTCAG 4 cut(s) 102, 168, 212, 306
BseRI GAGGAG 4 cut(s) 396, 473, 573, 576
BseXI GCAGC 2 cut(s) 499, 516
BshFI GGCC 5 cut(s) 141, 161, 226, 608, 617
BshNI GGYRCC 2 cut(s) 166, 302
BsiSI CCGG 1 cut(s) 320
BslFI GGGAC 1 cut(s) 269
BslI CCNNNNNNNGG 2 cut(s) 137, 407
BsmFI GGGAC 1 cut(s) 269
BsnI GGCC 5 cut(s) 141, 161, 226, 608, 617
Bsp1286I GDGCHC 1 cut(s) 189
Bsp1407I TGTACA 1 cut(s) 262
Bsp143I GATC 2 cut(s) 114, 625
Bsp1720I GCTNAGC 1 cut(s) 315
BspACI CCGC 2 cut(s) 405, 408
BspANI GGCC 5 cut(s) 141, 161, 226, 608, 617
BspCNI CTCAG 4 cut(s) 103, 169, 213, 307
BspLI GGNNCC 5 cut(s) 28, 59, 168, 304, 609
BspMAI CTGCAG 1 cut(s) 534
BspPI GGATC 1 cut(s) 109
BspQI GCTCTTC 2 cut(s) 19, 177
BspT107I GGYRCC 2 cut(s) 166, 302
BsrDI GCAATG 1 cut(s) 170
BsrFI RCCGGY 1 cut(s) 319
BsrGI TGTACA 1 cut(s) 262
BssAI RCCGGY 1 cut(s) 319
BssECI CCNNGG 1 cut(s) 162
BssMI GATC 2 cut(s) 114, 625
Bst2UI CCWGG 1 cut(s) 132
Bst4CI ACNGT 1 cut(s) 87
Bst6I CTCTTC 2 cut(s) 19, 177
BstAUI TGTACA 1 cut(s) 262
BstC8I GCNNGC 1 cut(s) 243
BstDEI CTNAG 6 cut(s) 99, 111, 177, 221, 315, 447
BstF5I GGATG 2 cut(s) 513, 642
BstKTI GATC 2 cut(s) 117, 628
BstMBI GATC 2 cut(s) 114, 625
BstMWI GCNNNNNNNGC 2 cut(s) 311, 614
BstNI CCWGG 1 cut(s) 132
BstNSI RCATGY 1 cut(s) 601
BstSCI CCNGG 1 cut(s) 130
BstSFI CTRYAG 2 cut(s) 83, 530
BstV1I GCAGC 2 cut(s) 499, 516
BstX2I RGATCY 1 cut(s) 114
BstYI RGATCY 1 cut(s) 114
Bsu36I CCTNAGG 1 cut(s) 177
BsuI GTATCC 1 cut(s) 89
BsuRI GGCC 5 cut(s) 141, 161, 226, 608, 617
BtsCI GGATG 2 cut(s) 513, 642
BtsI GCAGTG 1 cut(s) 637
BtsIMutI CAGTG 2 cut(s) 218, 637
Cac8I GCNNGC 1 cut(s) 243
CaiI CAGNNNCTG 1 cut(s) 71
Cfr10I RCCGGY 1 cut(s) 319
Cfr13I GGNCC 3 cut(s) 58, 95, 607
Csp6I GTAC 1 cut(s) 263
CviAII CATG 1 cut(s) 598
CviQI GTAC 1 cut(s) 263
DdeI CTNAG 6 cut(s) 99, 111, 177, 221, 315, 447
DpnI GATC 2 cut(s) 116, 627
DpnII GATC 2 cut(s) 114, 625
DraI TTTAAA 1 cut(s) 91
EaeI YGGCCR 1 cut(s) 615
Eam1104I CTCTTC 2 cut(s) 19, 177
EarI CTCTTC 2 cut(s) 19, 177
EciI GGCGGA 1 cut(s) 423
Eco147I AGGCCT 2 cut(s) 161, 226
Eco24I GRGCYC 1 cut(s) 189
Eco47I GGWCC 2 cut(s) 58, 95
Eco57I CTGAAG 2 cut(s) 330, 492
Eco81I CCTNAGG 1 cut(s) 177
EcoO109I RGGNCCY 3 cut(s) 58, 95, 607
EcoRII CCWGG 1 cut(s) 130
EcoT38I GRGCYC 1 cut(s) 189
FaeI CATG 1 cut(s) 601
FaiI YATR 5 cut(s) 138, 354, 599, 614, 620
FaqI GGGAC 1 cut(s) 269
FatI CATG 1 cut(s) 597
Fnu4HI GCNGC 3 cut(s) 406, 513, 530
FokI GGATG 1 cut(s) 520
FriOI GRGCYC 1 cut(s) 189
Fsp4HI GCNGC 3 cut(s) 406, 513, 530
FspBI CTAG 2 cut(s) 119, 195
GluI GCNGC 3 cut(s) 406, 513, 530
GsuI CTGGAG 1 cut(s) 577
HaeIII GGCC 5 cut(s) 141, 161, 226, 608, 617
HapII CCGG 1 cut(s) 320
Hin1II CATG 1 cut(s) 601
HindIII AAGCTT 1 cut(s) 431
HinfI GANTC 2 cut(s) 68, 257
HpaII CCGG 1 cut(s) 320
HphI GGTGA 1 cut(s) 334
Hpy188I TCNGA 5 cut(s) 112, 349, 438, 472, 492
Hpy188III TCNNGA 4 cut(s) 272, 378, 389, 555
HpyAV CCTTC 2 cut(s) 221, 467
HpyCH4III ACNGT 1 cut(s) 87
HpyCH4V TGCA 2 cut(s) 515, 532
HpyF10VI GCNNNNNNNGC 2 cut(s) 311, 614
HpyF3I CTNAG 6 cut(s) 99, 111, 177, 221, 315, 447
Hsp92II CATG 1 cut(s) 601
Kzo9I GATC 2 cut(s) 114, 625
LguI GCTCTTC 2 cut(s) 19, 177
Lsp1109I GCAGC 2 cut(s) 499, 516
MaeI CTAG 2 cut(s) 119, 195
MalI GATC 2 cut(s) 116, 627
MboI GATC 2 cut(s) 114, 625
MboII GAAGA 6 cut(s) 6, 194, 302, 320, 337, 580
MflI RGATCY 1 cut(s) 114
MhlI GDGCHC 1 cut(s) 189
MlsI TGGCCA 1 cut(s) 617
MluCI AATT 3 cut(s) 7, 236, 361
MluNI TGGCCA 1 cut(s) 617
MlyI GAGTC 1 cut(s) 251
MmeI TCCRAC 3 cut(s) 435, 551, 557
Mox20I TGGCCA 1 cut(s) 617
MroXI GAANNNNTTC 1 cut(s) 332
MscI TGGCCA 1 cut(s) 617
MseI TTAA 3 cut(s) 90, 191, 603
Msp20I TGGCCA 1 cut(s) 617
MspI CCGG 1 cut(s) 320
MspR9I CCNGG 1 cut(s) 132
MvaI CCWGG 1 cut(s) 132
MwoI GCNNNNNNNGC 2 cut(s) 311, 614
NdeII GATC 2 cut(s) 114, 625
NlaIII CATG 1 cut(s) 601
NlaIV GGNNCC 5 cut(s) 28, 59, 168, 304, 609
NspI RCATGY 1 cut(s) 601
PceI AGGCCT 2 cut(s) 161, 226
PciSI GCTCTTC 2 cut(s) 19, 177
PdmI GAANNNNTTC 1 cut(s) 332
PfeI GAWTC 1 cut(s) 68
PflMI CCANNNNNTGG 1 cut(s) 137
PkrI GCNGC 3 cut(s) 407, 514, 531
PleI GAGTC 1 cut(s) 251
PpsI GAGTC 1 cut(s) 251
PpuMI RGGWCCY 2 cut(s) 58, 95
Psp5II RGGWCCY 2 cut(s) 58, 95
Psp6I CCWGG 1 cut(s) 130
PspGI CCWGG 1 cut(s) 130
PspN4I GGNNCC 5 cut(s) 28, 59, 168, 304, 609
PspPI GGNCC 3 cut(s) 58, 95, 607
PspPPI RGGWCCY 2 cut(s) 58, 95
PstI CTGCAG 1 cut(s) 534
PstNI CAGNNNCTG 1 cut(s) 71
PsuI RGATCY 1 cut(s) 114
RsaI GTAC 1 cut(s) 264
RsaNI GTAC 1 cut(s) 263
SapI GCTCTTC 2 cut(s) 19, 177
SaqAI TTAA 3 cut(s) 90, 191, 603
SatI GCNGC 3 cut(s) 406, 513, 530
Sau3AI GATC 2 cut(s) 114, 625
Sau96I GGNCC 3 cut(s) 58, 95, 607
SchI GAGTC 1 cut(s) 251
ScrFI CCNGG 1 cut(s) 132
SduI GDGCHC 1 cut(s) 189
SfcI CTRYAG 2 cut(s) 83, 530
SfiI GGCCNNNNNGGCC 1 cut(s) 614
SinI GGWCC 2 cut(s) 58, 95
SpeI ACTAGT 1 cut(s) 194
Sse9I AATT 3 cut(s) 7, 236, 361
SseBI AGGCCT 2 cut(s) 161, 226
SsiI CCGC 2 cut(s) 405, 408
SspMI CTAG 2 cut(s) 119, 195
StuI AGGCCT 2 cut(s) 161, 226
StyD4I CCNGG 1 cut(s) 130
TaaI ACNGT 1 cut(s) 87
TasI AATT 3 cut(s) 7, 236, 361
TatI WGTACW 1 cut(s) 262
TauI GCSGC 1 cut(s) 408
TfiI GAWTC 1 cut(s) 68
Tru1I TTAA 3 cut(s) 90, 191, 603
Tru9I TTAA 3 cut(s) 90, 191, 603
TscAI CASTG 2 cut(s) 225, 637
TseI GCWGC 2 cut(s) 512, 529
TspRI CASTG 2 cut(s) 225, 637
Van91I CCANNNNNTGG 1 cut(s) 137
VpaK11BI GGWCC 2 cut(s) 58, 95
XapI RAATTY 1 cut(s) 361
XceI RCATGY 1 cut(s) 601
XmnI GAANNNNTTC 1 cut(s) 332
XspI CTAG 2 cut(s) 119, 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.