Rh7BG155200

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0010034
Physical Location & Seq
Forward (+)
11513096 .. 11514155
1060 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG155200.1

Sequence Viewer

Length: 414 bp
ATGGCGAAAACAACTAAGAAAGTCATGAAATACCATTCTAAAGCCAATAGCGCAAGTTTCCTAATCAAAGAAGGTGAGAAGGTGAAGAAACTTGCAGAGCAGTATGTTGAGACATCCCAAAAGGAGAAAGCTGCGATTGTGATCAAGGTAGGACAGAAATATTCACAACGGGAAATTGCTCCTCTCAAAGGAACCTATGGGGAGTGTCCTAACTTTATCAGGTTCCTTAAAGATTCTGGGGGTATCCTACAGTTTAAAGGTCCTAAGTTAGCTTCTGACATCCTCGCTGGTTTTCCAGGCTATGGCATTTTTCAGGAGGGCAAGGCCTCGGTGCCATTGCCTGAGGAAGAGCCCCTAACTTGTGGGCAATTCTACCTTCTTCCACTCAGGCCTAAAAAGGAAGTAGATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

137

Amino Acids

15.2

Weight (kDa)

9.43

Isoelectric Point (pI)

33.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 69 - 127 2.7e-06 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 331
AccB7I CCANNNNNTGG 1 cut(s) 302
AfiI CCNNNNNNNGG 2 cut(s) 188, 302
AjnI CCWGG 1 cut(s) 295
AluBI AGCT 2 cut(s) 131, 272
AluI AGCT 2 cut(s) 131, 272
Alw26I GTCTC 1 cut(s) 104
AoxI GGCC 2 cut(s) 324, 389
ApeKI GCWGC 1 cut(s) 131
AspLEI GCGC 1 cut(s) 53
AspS9I GGNCC 1 cut(s) 260
AsuHPI GGTGA 2 cut(s) 86, 94
AvaII GGWCC 1 cut(s) 260
AxyI CCTNAGG 1 cut(s) 342
BanI GGYRCC 1 cut(s) 331
BanII GRGCYC 1 cut(s) 354
BbvI GCAGC 1 cut(s) 118
BciT130I CCWGG 1 cut(s) 297
BciVI GTATCC 1 cut(s) 254
BclI TGATCA 1 cut(s) 141
BcoDI GTCTC 1 cut(s) 104
BfmI CTRYAG 1 cut(s) 248
BfuI GTATCC 1 cut(s) 254
BisI GCNGC 1 cut(s) 132
BlsI GCNGC 1 cut(s) 133
Bme1390I CCNGG 1 cut(s) 297
Bme18I GGWCC 1 cut(s) 260
BmgT120I GGNCC 1 cut(s) 260
BmiI GGNNCC 3 cut(s) 193, 224, 333
BmrFI CCNGG 1 cut(s) 297
BsaBI GATNNNNATC 1 cut(s) 140
BsaJI CCNNGG 1 cut(s) 327
Bsc4I CCNNNNNNNGG 2 cut(s) 188, 302
Bse21I CCTNAGG 1 cut(s) 342
Bse3DI GCAATG 1 cut(s) 335
Bse8I GATNNNNATC 1 cut(s) 140
BseBI CCWGG 1 cut(s) 297
BseDI CCNNGG 1 cut(s) 327
BseGI GGATG 2 cut(s) 113, 279
BseJI GATNNNNATC 1 cut(s) 140
BseLI CCNNNNNNNGG 2 cut(s) 188, 302
BseMI GCAATG 1 cut(s) 335
BseMII CTCAG 2 cut(s) 333, 400
BseRI GAGGAG 1 cut(s) 171
BseXI GCAGC 1 cut(s) 118
BshFI GGCC 2 cut(s) 326, 391
BshNI GGYRCC 1 cut(s) 331
BslI CCNNNNNNNGG 2 cut(s) 188, 302
BsmAI GTCTC 1 cut(s) 104
BsnI GGCC 2 cut(s) 326, 391
Bsp1286I GDGCHC 1 cut(s) 354
Bsp143I GATC 1 cut(s) 141
BspANI GGCC 2 cut(s) 326, 391
BspCNI CTCAG 2 cut(s) 334, 399
BspHI TCATGA 1 cut(s) 24
BspLI GGNNCC 3 cut(s) 193, 224, 333
BspQI GCTCTTC 1 cut(s) 342
BspT107I GGYRCC 1 cut(s) 331
BsrDI GCAATG 1 cut(s) 335
BssECI CCNNGG 1 cut(s) 327
BssMI GATC 1 cut(s) 141
Bst2UI CCWGG 1 cut(s) 297
Bst4CI ACNGT 1 cut(s) 252
Bst6I CTCTTC 1 cut(s) 342
BstDEI CTNAG 4 cut(s) 15, 264, 342, 386
BstENI CCTNNNNNAGG 1 cut(s) 186
BstF5I GGATG 2 cut(s) 113, 279
BstHHI GCGC 1 cut(s) 53
BstKTI GATC 1 cut(s) 144
BstMAI GTCTC 1 cut(s) 104
BstMBI GATC 1 cut(s) 141
BstMWI GCNNNNNNNGC 1 cut(s) 50
BstNI CCWGG 1 cut(s) 297
BstSCI CCNGG 1 cut(s) 295
BstSFI CTRYAG 1 cut(s) 248
BstV1I GCAGC 1 cut(s) 118
Bsu36I CCTNAGG 1 cut(s) 342
BsuI GTATCC 1 cut(s) 254
BsuRI GGCC 2 cut(s) 326, 391
BtsCI GGATG 2 cut(s) 113, 279
CciI TCATGA 1 cut(s) 24
CfoI GCGC 1 cut(s) 53
Cfr13I GGNCC 1 cut(s) 260
CviAII CATG 1 cut(s) 25
CviJI RGCY 7 cut(s) 44, 131, 272, 300, 326, 352, 391
CviKI_1 RGCY 7 cut(s) 44, 131, 272, 300, 326, 352, 391
DdeI CTNAG 4 cut(s) 15, 264, 342, 386
DpnI GATC 1 cut(s) 143
DpnII GATC 1 cut(s) 141
DraI TTTAAA 1 cut(s) 256
Eam1104I CTCTTC 1 cut(s) 342
EarI CTCTTC 1 cut(s) 342
Eco147I AGGCCT 2 cut(s) 326, 391
Eco24I GRGCYC 1 cut(s) 354
Eco47I GGWCC 1 cut(s) 260
Eco81I CCTNAGG 1 cut(s) 342
EcoNI CCTNNNNNAGG 1 cut(s) 186
EcoO109I RGGNCCY 1 cut(s) 260
EcoRII CCWGG 1 cut(s) 295
EcoT38I GRGCYC 1 cut(s) 354
FaeI CATG 1 cut(s) 28
FaiI YATR 4 cut(s) 26, 105, 198, 303
FatI CATG 1 cut(s) 24
FbaI TGATCA 1 cut(s) 141
Fnu4HI GCNGC 1 cut(s) 132
FokI GGATG 2 cut(s) 100, 266
FriOI GRGCYC 1 cut(s) 354
Fsp4HI GCNGC 1 cut(s) 132
GlaI GCGC 1 cut(s) 52
GluI GCNGC 1 cut(s) 132
HaeIII GGCC 2 cut(s) 326, 391
HhaI GCGC 1 cut(s) 53
Hin1II CATG 1 cut(s) 28
Hin6I GCGC 1 cut(s) 51
HinP1I GCGC 1 cut(s) 51
HinfI GANTC 2 cut(s) 233, 407
HphI GGTGA 2 cut(s) 86, 94
Hpy188I TCNGA 1 cut(s) 277
Hpy188III TCNNGA 2 cut(s) 25, 314
HpyAV CCTTC 3 cut(s) 65, 73, 386
HpyCH4III ACNGT 1 cut(s) 252
HpyCH4V TGCA 1 cut(s) 95
HpyF10VI GCNNNNNNNGC 1 cut(s) 50
HpyF3I CTNAG 4 cut(s) 15, 264, 342, 386
Hsp92II CATG 1 cut(s) 28
HspAI GCGC 1 cut(s) 51
Ksp22I TGATCA 1 cut(s) 141
Kzo9I GATC 1 cut(s) 141
LguI GCTCTTC 1 cut(s) 342
LmnI GCTCC 1 cut(s) 184
LpnPI CCDG 8 cut(s) 205, 222, 273, 282, 299, 309, 354, 373
Lsp1109I GCAGC 1 cut(s) 118
MalI GATC 1 cut(s) 143
MboI GATC 1 cut(s) 141
MboII GAAGA 3 cut(s) 97, 359, 371
MhlI GDGCHC 1 cut(s) 354
MluCI AATT 2 cut(s) 174, 368
MnlI CCTC 5 cut(s) 192, 293, 310, 337, 337
MseI TTAA 3 cut(s) 228, 255, 412
MspR9I CCNGG 1 cut(s) 297
MvaI CCWGG 1 cut(s) 297
MwoI GCNNNNNNNGC 1 cut(s) 50
NdeII GATC 1 cut(s) 141
NlaIII CATG 1 cut(s) 28
NlaIV GGNNCC 3 cut(s) 193, 224, 333
PagI TCATGA 1 cut(s) 24
PceI AGGCCT 2 cut(s) 326, 391
PciSI GCTCTTC 1 cut(s) 342
PfeI GAWTC 2 cut(s) 233, 407
PflMI CCANNNNNTGG 1 cut(s) 302
PkrI GCNGC 1 cut(s) 133
PpuMI RGGWCCY 1 cut(s) 260
Psp5II RGGWCCY 1 cut(s) 260
Psp6I CCWGG 1 cut(s) 295
PspGI CCWGG 1 cut(s) 295
PspN4I GGNNCC 3 cut(s) 193, 224, 333
PspPI GGNCC 1 cut(s) 260
PspPPI RGGWCCY 1 cut(s) 260
SapI GCTCTTC 1 cut(s) 342
SaqAI TTAA 3 cut(s) 228, 255, 412
SatI GCNGC 1 cut(s) 132
Sau3AI GATC 1 cut(s) 141
Sau96I GGNCC 1 cut(s) 260
ScrFI CCNGG 1 cut(s) 297
SduI GDGCHC 1 cut(s) 354
SetI ASST 9 cut(s) 76, 84, 133, 150, 197, 224, 262, 274, 378
SfcI CTRYAG 1 cut(s) 248
SinI GGWCC 1 cut(s) 260
Sse9I AATT 2 cut(s) 174, 368
SseBI AGGCCT 2 cut(s) 326, 391
SspI AATATT 1 cut(s) 161
StuI AGGCCT 2 cut(s) 326, 391
StyD4I CCNGG 1 cut(s) 295
TaaI ACNGT 1 cut(s) 252
TasI AATT 2 cut(s) 174, 368
TfiI GAWTC 2 cut(s) 233, 407
Tru1I TTAA 3 cut(s) 228, 255, 412
Tru9I TTAA 3 cut(s) 228, 255, 412
TseI GCWGC 1 cut(s) 131
TspDTI ATGAA 1 cut(s) 41
Van91I CCANNNNNTGG 1 cut(s) 302
VpaK11BI GGWCC 1 cut(s) 260
XagI CCTNNNNNAGG 1 cut(s) 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.