MD06G1110100.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0010034
Physical Location & Seq
Reverse (-)
24936027 .. 24936788
762 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1110100.v1.1.491

Sequence Viewer

Length: 762 bp
ATGGGGAACTGTTCATCACTCACAGGAACCTCTGGGGAGTGTCCTAAATCTATTAGGGTTCTTACAGATTCTGGGGCTATGCTAGAATTTAAAGGCCCTAAACTAGCTGCTGAAATCCTCACTGGTTTTCCAGGCTATGGCATTTTCCAACAGGGCAAGGCCTCCTTGCCAATGTCTGACCAAGAAACCCTAATTAGTGGCCAGTACTATCTTCTTCCGCTGAAGCCACAGCCACTGGTTTGCGAACATGGGGTTGCTGAGAAACCAGAGGCTGCGGAAGCTGAGCCTGTGAAGACGTCATTTGGTGCACTACCGGATTTCGTGGAGAATTCGGCAAACGGGTCATCAAATCTTGGAGTTGAAGTCCTGGAGAAGGCTGCAGAAGCTGAGTCTGTGAAGAAGTCATTTGGAGCAGGATCAGAAGTTGAAGTCATGGAGGCTGCTGAAGCTGAGCCTGTGAAGAAGTCATTTTGTGCAGGATCAGATTTCGTGGAGAATTTGGCGAAAGGGTCGTCGGCTCTTGAGGTGCTGCCGTCTGGTGGCGACGGGGTTTGGAGGGTGAAGCTGGTGATAGACACTAAGCAACTGGAGGAGTTATTTTCCGAAAATGGCGACGCAGGGGCGCTGATTGAGACGATGAGGATGGCTGCAAGTGAAGGAGCAGGGGCAAGCACTCCAAGGCAGACAAAGAGCCTTTGGGGAGGAGCAGGAGGTTGGAGGAAGCCCATCTTCTCCAACTTGTTTAAGGTGCCCCCCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

254

Amino Acids

26.48

Weight (kDa)

4.8

Isoelectric Point (pI)

28.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 217 9.3e-13 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 299
AccB1I GGYRCC 1 cut(s) 748
AccB7I CCANNNNNTGG 1 cut(s) 137
AciI CCGC 2 cut(s) 218, 275
AclWI GGATC 2 cut(s) 424, 487
AcoI YGGCCR 1 cut(s) 199
AcsI RAATTY 3 cut(s) 86, 328, 496
AcuI CTGAAG 2 cut(s) 242, 465
AcyI GRCGYC 1 cut(s) 296
AfaI GTAC 1 cut(s) 206
AfiI CCNNNNNNNGG 3 cut(s) 137, 373, 539
AgsI TTSAA 2 cut(s) 362, 428
AjnI CCWGG 2 cut(s) 130, 366
AluBI AGCT 5 cut(s) 107, 281, 386, 449, 565
AluI AGCT 5 cut(s) 107, 281, 386, 449, 565
Alw21I GWGCWC 1 cut(s) 310
Alw26I GTCTC 1 cut(s) 626
Alw44I GTGCAC 1 cut(s) 306
AlwI GGATC 2 cut(s) 424, 487
AlwNI CAGNNNCTG 4 cut(s) 71, 235, 272, 386
AoxI GGCC 3 cut(s) 94, 159, 199
ApaLI GTGCAC 1 cut(s) 306
ApeKI GCWGC 6 cut(s) 107, 272, 377, 440, 529, 647
ApoI RAATTY 3 cut(s) 86, 328, 496
ArsI GACNNNNNNTTYG 2 cut(s) 497, 529
AspLEI GCGC 1 cut(s) 625
AspS9I GGNCC 1 cut(s) 95
AsuHPI GGTGA 2 cut(s) 571, 580
BaeGI GKGCMC 2 cut(s) 310, 753
BalI TGGCCA 1 cut(s) 201
BanI GGYRCC 1 cut(s) 748
BbsI GAAGAC 1 cut(s) 299
Bbv12I GWGCWC 1 cut(s) 310
BbvI GCAGC 6 cut(s) 94, 259, 364, 427, 516, 634
BccI CCATC 2 cut(s) 637, 734
BceAI ACGGC 1 cut(s) 517
BciT130I CCWGG 2 cut(s) 132, 368
BcoDI GTCTC 1 cut(s) 626
BfaI CTAG 2 cut(s) 83, 104
BfmI CTRYAG 1 cut(s) 378
BfoI RGCGCY 1 cut(s) 626
BisI GCNGC 6 cut(s) 108, 273, 378, 441, 530, 648
BlpI GCTNAGC 2 cut(s) 282, 450
BlsI GCNGC 6 cut(s) 109, 274, 379, 442, 531, 649
BmcAI AGTACT 1 cut(s) 206
Bme1390I CCNGG 2 cut(s) 132, 368
BmgT120I GGNCC 1 cut(s) 95
BmiI GGNNCC 2 cut(s) 28, 750
BmrFI CCNGG 2 cut(s) 132, 368
BpiI GAAGAC 1 cut(s) 299
BpmI CTGGAG 2 cut(s) 389, 608
Bpu1102I GCTNAGC 2 cut(s) 282, 450
BpuEI CTTGAG 1 cut(s) 542
BsaHI GRCGYC 1 cut(s) 296
BsaJI CCNNGG 1 cut(s) 677
BsaWI WCCGGW 1 cut(s) 313
BsaXI ACNNNNNCTCC 2 cut(s) 348, 378
Bsc4I CCNNNNNNNGG 3 cut(s) 137, 373, 539
Bse1I ACTGG 4 cut(s) 127, 202, 240, 591
BseBI CCWGG 2 cut(s) 132, 368
BseDI CCNNGG 1 cut(s) 677
BseGI GGATG 1 cut(s) 648
BseLI CCNNNNNNNGG 3 cut(s) 137, 373, 539
BseMII CTCAG 4 cut(s) 249, 273, 378, 441
BseNI ACTGG 4 cut(s) 127, 202, 240, 591
BseRI GAGGAG 2 cut(s) 605, 717
BseSI GKGCMC 2 cut(s) 310, 753
BseXI GCAGC 6 cut(s) 94, 259, 364, 427, 516, 634
BsgI GTGCAG 1 cut(s) 495
BshFI GGCC 3 cut(s) 96, 161, 201
BshNI GGYRCC 1 cut(s) 748
BsiHKAI GWGCWC 1 cut(s) 310
BsiSI CCGG 1 cut(s) 314
BslI CCNNNNNNNGG 3 cut(s) 137, 373, 539
BsmAI GTCTC 1 cut(s) 626
BsmBI CGTCTC 1 cut(s) 626
BsnI GGCC 3 cut(s) 96, 161, 201
Bsp1286I GDGCHC 2 cut(s) 310, 753
Bsp143I GATC 2 cut(s) 416, 479
Bsp1720I GCTNAGC 2 cut(s) 282, 450
BspACI CCGC 2 cut(s) 218, 275
BspANI GGCC 3 cut(s) 96, 161, 201
BspCNI CTCAG 4 cut(s) 250, 274, 379, 442
BspLI GGNNCC 2 cut(s) 28, 750
BspMAI CTGCAG 1 cut(s) 382
BspPI GGATC 2 cut(s) 424, 487
BspT107I GGYRCC 1 cut(s) 748
BsrI ACTGG 4 cut(s) 127, 202, 240, 591
BssECI CCNNGG 1 cut(s) 677
BssMI GATC 2 cut(s) 416, 479
BssNI GRCGYC 1 cut(s) 296
BssT1I CCWWGG 1 cut(s) 677
Bst2UI CCWGG 2 cut(s) 132, 368
Bst4CI ACNGT 1 cut(s) 11
BstACI GRCGYC 1 cut(s) 296
BstC8I GCNNGC 1 cut(s) 670
BstDEI CTNAG 5 cut(s) 258, 282, 387, 450, 579
BstENI CCTNNNNNAGG 1 cut(s) 371
BstF5I GGATG 1 cut(s) 648
BstH2I RGCGCY 1 cut(s) 626
BstHHI GCGC 1 cut(s) 625
BstKTI GATC 2 cut(s) 419, 482
BstMAI GTCTC 1 cut(s) 626
BstMBI GATC 2 cut(s) 416, 479
BstMWI GCNNNNNNNGC 3 cut(s) 278, 383, 446
BstNI CCWGG 2 cut(s) 132, 368
BstSCI CCNGG 2 cut(s) 130, 366
BstSFI CTRYAG 1 cut(s) 378
BstSLI GKGCMC 2 cut(s) 310, 753
BstV1I GCAGC 6 cut(s) 94, 259, 364, 427, 516, 634
BstV2I GAAGAC 1 cut(s) 299
BsuRI GGCC 3 cut(s) 96, 161, 201
BtsCI GGATG 1 cut(s) 648
BtsIMutI CAGTG 2 cut(s) 120, 233
Cac8I GCNNGC 1 cut(s) 670
CaiI CAGNNNCTG 4 cut(s) 71, 235, 272, 386
CfoI GCGC 1 cut(s) 625
Cfr13I GGNCC 1 cut(s) 95
CseI GACGC 1 cut(s) 623
Csp6I GTAC 1 cut(s) 205
CviAII CATG 2 cut(s) 248, 433
CviQI GTAC 1 cut(s) 205
DdeI CTNAG 5 cut(s) 258, 282, 387, 450, 579
DpnI GATC 2 cut(s) 418, 481
DpnII GATC 2 cut(s) 416, 479
DraI TTTAAA 1 cut(s) 91
EaeI YGGCCR 1 cut(s) 199
Eco130I CCWWGG 1 cut(s) 677
Eco147I AGGCCT 1 cut(s) 161
Eco57I CTGAAG 2 cut(s) 242, 465
EcoNI CCTNNNNNAGG 1 cut(s) 371
EcoO109I RGGNCCY 1 cut(s) 95
EcoRI GAATTC 1 cut(s) 328
EcoRII CCWGG 2 cut(s) 130, 366
EcoT14I CCWWGG 1 cut(s) 677
ErhI CCWWGG 1 cut(s) 677
Esp3I CGTCTC 1 cut(s) 626
FaeI CATG 2 cut(s) 251, 436
FaiI YATR 4 cut(s) 80, 138, 249, 434
FalI AAGNNNNNCTT 4 cut(s) 149, 181, 713, 745
FatI CATG 2 cut(s) 247, 432
Fnu4HI GCNGC 6 cut(s) 108, 273, 378, 441, 530, 648
FokI GGATG 1 cut(s) 655
Fsp4HI GCNGC 6 cut(s) 108, 273, 378, 441, 530, 648
FspBI CTAG 2 cut(s) 83, 104
GlaI GCGC 1 cut(s) 624
GluI GCNGC 6 cut(s) 108, 273, 378, 441, 530, 648
GsuI CTGGAG 2 cut(s) 389, 608
HaeII RGCGCY 1 cut(s) 626
HaeIII GGCC 3 cut(s) 96, 161, 201
HapII CCGG 1 cut(s) 314
HgaI GACGC 1 cut(s) 623
HhaI GCGC 1 cut(s) 625
Hin1I GRCGYC 1 cut(s) 296
Hin1II CATG 2 cut(s) 251, 436
Hin6I GCGC 1 cut(s) 623
HinP1I GCGC 1 cut(s) 623
HinfI GANTC 2 cut(s) 68, 389
HpaII CCGG 1 cut(s) 314
HphI GGTGA 2 cut(s) 571, 580
Hpy166II GTNNAC 1 cut(s) 308
Hpy188I TCNGA 4 cut(s) 178, 421, 484, 604
Hpy188III TCNNGA 1 cut(s) 521
Hpy8I GTNNAC 1 cut(s) 308
Hpy99I CGWCG 3 cut(s) 517, 548, 617
HpyAV CCTTC 2 cut(s) 367, 650
HpyCH4III ACNGT 1 cut(s) 11
HpyCH4IV ACGT 1 cut(s) 296
HpyCH4V TGCA 4 cut(s) 308, 380, 476, 650
HpyF10VI GCNNNNNNNGC 3 cut(s) 278, 383, 446
HpyF3I CTNAG 5 cut(s) 258, 282, 387, 450, 579
HpySE526I ACGT 1 cut(s) 296
Hsp92I GRCGYC 1 cut(s) 296
Hsp92II CATG 2 cut(s) 251, 436
HspAI GCGC 1 cut(s) 623
Kzo9I GATC 2 cut(s) 416, 479
LmnI GCTCC 3 cut(s) 410, 659, 704
Lsp1109I GCAGC 6 cut(s) 94, 259, 364, 427, 516, 634
MaeI CTAG 2 cut(s) 83, 104
MaeII ACGT 1 cut(s) 296
MalI GATC 2 cut(s) 418, 481
MboI GATC 2 cut(s) 416, 479
MboII GAAGA 6 cut(s) 203, 206, 304, 409, 472, 721
MhlI GDGCHC 2 cut(s) 310, 753
MlsI TGGCCA 1 cut(s) 201
MluCI AATT 4 cut(s) 86, 192, 328, 496
MluNI TGGCCA 1 cut(s) 201
MlyI GAGTC 1 cut(s) 398
MmeI TCCRAC 3 cut(s) 172, 695, 759
Mox20I TGGCCA 1 cut(s) 201
MscI TGGCCA 1 cut(s) 201
MseI TTAA 2 cut(s) 90, 744
Msp20I TGGCCA 1 cut(s) 201
MspA1I CMGCKG 1 cut(s) 220
MspI CCGG 1 cut(s) 314
MspR9I CCNGG 2 cut(s) 132, 368
MvaI CCWGG 2 cut(s) 132, 368
MwoI GCNNNNNNNGC 3 cut(s) 278, 383, 446
NdeII GATC 2 cut(s) 416, 479
NlaIII CATG 2 cut(s) 251, 436
NlaIV GGNNCC 2 cut(s) 28, 750
PceI AGGCCT 1 cut(s) 161
PfeI GAWTC 1 cut(s) 68
PflMI CCANNNNNTGG 1 cut(s) 137
PfoI TCCNGGA 1 cut(s) 366
PkrI GCNGC 6 cut(s) 109, 274, 379, 442, 531, 649
PleI GAGTC 1 cut(s) 397
PpsI GAGTC 1 cut(s) 397
Psp6I CCWGG 2 cut(s) 130, 366
PspGI CCWGG 2 cut(s) 130, 366
PspN4I GGNNCC 2 cut(s) 28, 750
PspPI GGNCC 1 cut(s) 95
PstI CTGCAG 1 cut(s) 382
PstNI CAGNNNCTG 4 cut(s) 71, 235, 272, 386
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
SaqAI TTAA 2 cut(s) 90, 744
SatI GCNGC 6 cut(s) 108, 273, 378, 441, 530, 648
Sau3AI GATC 2 cut(s) 416, 479
Sau96I GGNCC 1 cut(s) 95
ScaI AGTACT 1 cut(s) 206
SchI GAGTC 1 cut(s) 398
ScrFI CCNGG 2 cut(s) 132, 368
SduI GDGCHC 2 cut(s) 310, 753
SfcI CTRYAG 1 cut(s) 378
SmlI CTYRAG 1 cut(s) 521
SmoI CTYRAG 1 cut(s) 521
Sse9I AATT 4 cut(s) 86, 192, 328, 496
SseBI AGGCCT 1 cut(s) 161
SsiI CCGC 2 cut(s) 218, 275
SspMI CTAG 2 cut(s) 83, 104
StuI AGGCCT 1 cut(s) 161
StyD4I CCNGG 2 cut(s) 130, 366
StyI CCWWGG 1 cut(s) 677
TaaI ACNGT 1 cut(s) 11
TaiI ACGT 1 cut(s) 299
TasI AATT 4 cut(s) 86, 192, 328, 496
TatI WGTACW 1 cut(s) 204
TfiI GAWTC 1 cut(s) 68
Tru1I TTAA 2 cut(s) 90, 744
Tru9I TTAA 2 cut(s) 90, 744
TscAI CASTG 2 cut(s) 127, 240
TseI GCWGC 6 cut(s) 107, 272, 377, 440, 529, 647
TspRI CASTG 2 cut(s) 127, 240
Van91I CCANNNNNTGG 1 cut(s) 137
VneI GTGCAC 1 cut(s) 306
XagI CCTNNNNNAGG 1 cut(s) 371
XapI RAATTY 3 cut(s) 86, 328, 496
XspI CTAG 2 cut(s) 83, 104
ZraI GACGTC 1 cut(s) 297
ZrmI AGTACT 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.