MD14G1131100.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0010034
Physical Location & Seq
Reverse (-)
20855336 .. 20856079
744 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1131100.v1.1.491

Sequence Viewer

Length: 744 bp
ATGGGGAATTGTTCTTCTCTCAAAGGAACCACTGGGGAGTGTCCTAAATCTATTAGGGTTCTTACAGATTCTGGGGGTGTCCTAGAATTTAAAGGCCCTATACTAGCTGCTGAAATCCTCACTGGTTTTCCACGCTATGGCATTTTCCAACAAGGCAAGGCCGCCTTGCCAATGTCTGACCAAGAAACCCTTACTAGTGGCCAGTACTACCTTCTTCCACTGAAGGCAGACCAACCCTTTTGCCGAAATCGGGTTGCTGAGAAACCGGTGGCTGCAGAAGCTGAGCCTGTGGAGAAGTCATTTGGTGCAGAATCCGATTTCATGGAGAATTTGGTGAACGGGTTATCAGGTCTTGGAGTTGAAGTCCTGGAGAAGGCTGCAGAACGTGAGCCTGTGAAGATGTCATGTGGTGCAGGATCAGAAGTTGGAGTCATGGAGGCTGCTGAAGCTGAGCCAGTGAAGAAGTCATTTTGTGCAGGATCAGATTTCGTGGAGAATTTGGCGAAAGGGTCATCGGCTCTTGAGGTGCTGCCTTCTGGTGGAGACGGGGTTTGGAGGGTGAAGCTGGTGATCGAGACTAAGCAATTGGAGGAGTTATTTTCCGAAAATGGCGATGCTGGGGCGTTGATTGAGACGATGAGGATGGCTGCAAGTGGAGGCTGGGCAAGCACTCCAAAGAAGACAAAGAGCCCTTGGGGAGGAGGTGGTGGTTGGAGGAAGTCTAACTTCTCCAACTTGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

248

Amino Acids

26.09

Weight (kDa)

5.02

Isoelectric Point (pI)

38.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 203 2.5e-13 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 137
AciI CCGC 1 cut(s) 162
AclWI GGATC 2 cut(s) 424, 487
AcoI YGGCCR 1 cut(s) 199
AcsI RAATTY 3 cut(s) 86, 328, 496
AcuI CTGAAG 2 cut(s) 242, 465
AfaI GTAC 1 cut(s) 206
AfiI CCNNNNNNNGG 5 cut(s) 137, 250, 373, 539, 698
AgeI ACCGGT 1 cut(s) 265
AgsI TTSAA 1 cut(s) 362
AhlI ACTAGT 1 cut(s) 194
AjnI CCWGG 1 cut(s) 366
AluBI AGCT 4 cut(s) 107, 281, 449, 565
AluI AGCT 4 cut(s) 107, 281, 449, 565
Alw26I GTCTC 3 cut(s) 537, 569, 626
AlwI GGATC 2 cut(s) 424, 487
AlwNI CAGNNNCTG 2 cut(s) 71, 281
AoxI GGCC 3 cut(s) 94, 159, 199
ApeKI GCWGC 6 cut(s) 107, 272, 377, 440, 529, 647
ApoI RAATTY 3 cut(s) 86, 328, 496
AsiGI ACCGGT 1 cut(s) 265
AspS9I GGNCC 1 cut(s) 95
AsuHPI GGTGA 3 cut(s) 346, 571, 580
BalI TGGCCA 1 cut(s) 201
BanII GRGCYC 1 cut(s) 692
BbsI GAAGAC 1 cut(s) 686
BbvI GCAGC 6 cut(s) 94, 259, 364, 427, 516, 634
BccI CCATC 1 cut(s) 637
BciT130I CCWGG 1 cut(s) 368
BcoDI GTCTC 3 cut(s) 537, 569, 626
BcuI ACTAGT 1 cut(s) 194
BfaI CTAG 3 cut(s) 83, 104, 195
BfmI CTRYAG 2 cut(s) 273, 378
BisI GCNGC 7 cut(s) 108, 162, 273, 378, 441, 530, 648
BlpI GCTNAGC 2 cut(s) 282, 450
BlsI GCNGC 7 cut(s) 109, 163, 274, 379, 442, 531, 649
BmcAI AGTACT 1 cut(s) 206
Bme1390I CCNGG 1 cut(s) 368
BmgT120I GGNCC 1 cut(s) 95
BmiI GGNNCC 1 cut(s) 28
BmrFI CCNGG 1 cut(s) 368
BmrI ACTGGG 1 cut(s) 42
BmsI GCATC 1 cut(s) 604
BmuI ACTGGG 1 cut(s) 42
BpiI GAAGAC 1 cut(s) 686
BpmI CTGGAG 1 cut(s) 389
Bpu1102I GCTNAGC 2 cut(s) 282, 450
BpuEI CTTGAG 1 cut(s) 542
BsaJI CCNNGG 1 cut(s) 692
BsaWI WCCGGW 1 cut(s) 265
BsaXI ACNNNNNCTCC 4 cut(s) 348, 378, 534, 564
Bsc4I CCNNNNNNNGG 5 cut(s) 137, 250, 373, 539, 698
Bse118I RCCGGY 1 cut(s) 265
Bse1I ACTGG 4 cut(s) 37, 127, 202, 455
BseBI CCWGG 1 cut(s) 368
BseDI CCNNGG 1 cut(s) 692
BseGI GGATG 1 cut(s) 648
BseLI CCNNNNNNNGG 5 cut(s) 137, 250, 373, 539, 698
BseMII CTCAG 3 cut(s) 249, 273, 441
BseNI ACTGG 4 cut(s) 37, 127, 202, 455
BseRI GAGGAG 2 cut(s) 605, 714
BseXI GCAGC 6 cut(s) 94, 259, 364, 427, 516, 634
BseYI CCCAGC 2 cut(s) 617, 660
BsgI GTGCAG 3 cut(s) 327, 432, 495
BshFI GGCC 3 cut(s) 96, 161, 201
BshTI ACCGGT 1 cut(s) 265
BsiSI CCGG 1 cut(s) 266
BslI CCNNNNNNNGG 5 cut(s) 137, 250, 373, 539, 698
BsmAI GTCTC 3 cut(s) 537, 569, 626
BsmBI CGTCTC 2 cut(s) 537, 626
BsnI GGCC 3 cut(s) 96, 161, 201
Bsp1286I GDGCHC 1 cut(s) 692
Bsp143I GATC 3 cut(s) 416, 479, 570
Bsp1720I GCTNAGC 2 cut(s) 282, 450
BspACI CCGC 1 cut(s) 162
BspANI GGCC 3 cut(s) 96, 161, 201
BspCNI CTCAG 3 cut(s) 250, 274, 442
BspLI GGNNCC 1 cut(s) 28
BspMAI CTGCAG 2 cut(s) 277, 382
BspPI GGATC 2 cut(s) 424, 487
BsrFI RCCGGY 1 cut(s) 265
BsrI ACTGG 4 cut(s) 37, 127, 202, 455
BssAI RCCGGY 1 cut(s) 265
BssECI CCNNGG 1 cut(s) 692
BssMI GATC 3 cut(s) 416, 479, 570
BssT1I CCWWGG 1 cut(s) 692
Bst2UI CCWGG 1 cut(s) 368
BstC8I GCNNGC 1 cut(s) 667
BstDEI CTNAG 4 cut(s) 258, 282, 450, 579
BstENI CCTNNNNNAGG 2 cut(s) 371, 696
BstF5I GGATG 1 cut(s) 648
BstKTI GATC 3 cut(s) 419, 482, 573
BstMAI GTCTC 3 cut(s) 537, 569, 626
BstMBI GATC 3 cut(s) 416, 479, 570
BstMWI GCNNNNNNNGC 3 cut(s) 278, 446, 666
BstNI CCWGG 1 cut(s) 368
BstSCI CCNGG 1 cut(s) 366
BstSFI CTRYAG 2 cut(s) 273, 378
BstV1I GCAGC 6 cut(s) 94, 259, 364, 427, 516, 634
BstV2I GAAGAC 1 cut(s) 686
BsuRI GGCC 3 cut(s) 96, 161, 201
BtgZI GCGATG 1 cut(s) 627
BtsCI GGATG 1 cut(s) 648
BtsIMutI CAGTG 4 cut(s) 30, 120, 218, 462
Cac8I GCNNGC 1 cut(s) 667
CaiI CAGNNNCTG 2 cut(s) 71, 281
Cfr10I RCCGGY 1 cut(s) 265
Cfr13I GGNCC 1 cut(s) 95
Csp6I GTAC 1 cut(s) 205
CspAI ACCGGT 1 cut(s) 265
CviAII CATG 3 cut(s) 322, 405, 433
CviQI GTAC 1 cut(s) 205
DdeI CTNAG 4 cut(s) 258, 282, 450, 579
DpnI GATC 3 cut(s) 418, 481, 572
DpnII GATC 3 cut(s) 416, 479, 570
DraI TTTAAA 1 cut(s) 91
EaeI YGGCCR 1 cut(s) 199
Eco130I CCWWGG 1 cut(s) 692
Eco24I GRGCYC 1 cut(s) 692
Eco57I CTGAAG 2 cut(s) 242, 465
EcoNI CCTNNNNNAGG 2 cut(s) 371, 696
EcoO109I RGGNCCY 1 cut(s) 95
EcoRII CCWGG 1 cut(s) 366
EcoT14I CCWWGG 1 cut(s) 692
EcoT38I GRGCYC 1 cut(s) 692
ErhI CCWWGG 1 cut(s) 692
Esp3I CGTCTC 2 cut(s) 537, 626
FaeI CATG 3 cut(s) 325, 408, 436
FaiI YATR 5 cut(s) 101, 138, 323, 406, 434
FalI AAGNNNNNCTT 6 cut(s) 149, 181, 174, 206, 710, 742
FatI CATG 3 cut(s) 321, 404, 432
Fnu4HI GCNGC 7 cut(s) 108, 162, 273, 378, 441, 530, 648
FokI GGATG 1 cut(s) 655
FriOI GRGCYC 1 cut(s) 692
Fsp4HI GCNGC 7 cut(s) 108, 162, 273, 378, 441, 530, 648
FspBI CTAG 3 cut(s) 83, 104, 195
GluI GCNGC 7 cut(s) 108, 162, 273, 378, 441, 530, 648
GsaI CCCAGC 2 cut(s) 621, 664
GsuI CTGGAG 1 cut(s) 389
HaeIII GGCC 3 cut(s) 96, 161, 201
HapII CCGG 1 cut(s) 266
Hin1II CATG 3 cut(s) 325, 408, 436
HinfI GANTC 3 cut(s) 68, 311, 429
HpaII CCGG 1 cut(s) 266
HphI GGTGA 3 cut(s) 346, 571, 580
Hpy166II GTNNAC 1 cut(s) 337
Hpy188I TCNGA 5 cut(s) 178, 316, 421, 484, 604
Hpy188III TCNNGA 2 cut(s) 521, 574
Hpy8I GTNNAC 1 cut(s) 337
HpyAV CCTTC 4 cut(s) 217, 221, 367, 543
HpyCH4IV ACGT 1 cut(s) 385
HpyCH4V TGCA 6 cut(s) 275, 308, 380, 413, 476, 650
HpyF10VI GCNNNNNNNGC 3 cut(s) 278, 446, 666
HpyF3I CTNAG 4 cut(s) 258, 282, 450, 579
HpySE526I ACGT 1 cut(s) 385
Hsp92II CATG 3 cut(s) 325, 408, 436
Kzo9I GATC 3 cut(s) 416, 479, 570
Lsp1109I GCAGC 6 cut(s) 94, 259, 364, 427, 516, 634
LweI GCATC 1 cut(s) 604
MaeI CTAG 3 cut(s) 83, 104, 195
MaeII ACGT 1 cut(s) 385
MalI GATC 3 cut(s) 418, 481, 572
MboI GATC 3 cut(s) 416, 479, 570
MboII GAAGA 5 cut(s) 6, 206, 409, 472, 691
MfeI CAATTG 1 cut(s) 584
MhlI GDGCHC 1 cut(s) 692
MlsI TGGCCA 1 cut(s) 201
MluCI AATT 5 cut(s) 7, 86, 328, 496, 584
MluNI TGGCCA 1 cut(s) 201
MlyI GAGTC 1 cut(s) 438
MmeI TCCRAC 3 cut(s) 172, 406, 692
Mox20I TGGCCA 1 cut(s) 201
MscI TGGCCA 1 cut(s) 201
MseI TTAA 2 cut(s) 90, 742
Msp20I TGGCCA 1 cut(s) 201
MspI CCGG 1 cut(s) 266
MspR9I CCNGG 1 cut(s) 368
MunI CAATTG 1 cut(s) 584
MvaI CCWGG 1 cut(s) 368
MwoI GCNNNNNNNGC 3 cut(s) 278, 446, 666
NdeII GATC 3 cut(s) 416, 479, 570
NlaIII CATG 3 cut(s) 325, 408, 436
NlaIV GGNNCC 1 cut(s) 28
PfeI GAWTC 2 cut(s) 68, 311
PflMI CCANNNNNTGG 1 cut(s) 137
PfoI TCCNGGA 1 cut(s) 366
PinAI ACCGGT 1 cut(s) 265
PkrI GCNGC 7 cut(s) 109, 163, 274, 379, 442, 531, 649
PleI GAGTC 1 cut(s) 437
PpsI GAGTC 1 cut(s) 437
Psp6I CCWGG 1 cut(s) 366
PspFI CCCAGC 2 cut(s) 617, 660
PspGI CCWGG 1 cut(s) 366
PspN4I GGNNCC 1 cut(s) 28
PspPI GGNCC 1 cut(s) 95
PstI CTGCAG 2 cut(s) 277, 382
PstNI CAGNNNCTG 2 cut(s) 71, 281
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
SaqAI TTAA 2 cut(s) 90, 742
SatI GCNGC 7 cut(s) 108, 162, 273, 378, 441, 530, 648
Sau3AI GATC 3 cut(s) 416, 479, 570
Sau96I GGNCC 1 cut(s) 95
ScaI AGTACT 1 cut(s) 206
SchI GAGTC 1 cut(s) 438
ScrFI CCNGG 1 cut(s) 368
SduI GDGCHC 1 cut(s) 692
SetI ASST 9 cut(s) 109, 213, 283, 352, 388, 451, 528, 567, 706
SfaNI GCATC 1 cut(s) 604
SfcI CTRYAG 2 cut(s) 273, 378
SmlI CTYRAG 1 cut(s) 521
SmoI CTYRAG 1 cut(s) 521
SpeI ACTAGT 1 cut(s) 194
Sse9I AATT 5 cut(s) 7, 86, 328, 496, 584
SsiI CCGC 1 cut(s) 162
SspMI CTAG 3 cut(s) 83, 104, 195
StyD4I CCNGG 1 cut(s) 366
StyI CCWWGG 1 cut(s) 692
TaiI ACGT 1 cut(s) 388
TaqI TCGA 1 cut(s) 573
TasI AATT 5 cut(s) 7, 86, 328, 496, 584
TatI WGTACW 1 cut(s) 204
TauI GCSGC 1 cut(s) 164
TfiI GAWTC 2 cut(s) 68, 311
Tru1I TTAA 2 cut(s) 90, 742
Tru9I TTAA 2 cut(s) 90, 742
TscAI CASTG 4 cut(s) 37, 127, 225, 462
TseI GCWGC 6 cut(s) 107, 272, 377, 440, 529, 647
TspDTI ATGAA 1 cut(s) 310
TspRI CASTG 4 cut(s) 37, 127, 225, 462
Van91I CCANNNNNTGG 1 cut(s) 137
XagI CCTNNNNNAGG 2 cut(s) 371, 696
XapI RAATTY 3 cut(s) 86, 328, 496
XspI CTAG 3 cut(s) 83, 104, 195
ZrmI AGTACT 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.