Rw7G012060

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0010034
Physical Location & Seq
Forward (+)
11427227 .. 11428485
1259 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G012060.1

Sequence Viewer

Length: 678 bp
ATGATCGACCGCTCCGATTGTGGTCAAGGTGGGAAAGAAATATTCACAATGGGAAATTGCTCTTCTCTCAAAGGAACCTGTGGGGAGTGTCGTAACTCTATCAGGGTCCTTACAGATTCTGGGGGTATCCTACAGTTTAAAGGTCCTAAGTTAGCTTCGGAAATCCTCGCTGGTTTTCCAGGCTATGGCATTTTTCAGCAGGGCAAGGCCTCGGTGCCATTGCCTGAGGAAGAGGCCTTAACTAGTGGCCAGTTCTACCTTCGTCCACTGAGGACTAAAGAGGAAGTGCCTGCTGATTATGGGGTCTCTGTAGAAGTTCAAGAAATGGGTGCTGATGAAGATTTGGATGGTGCAGAAGAAGCTGAGCCGGTGAAGAAGTCGTTTGGGGGTGCATCGGATTGCGTGGAGAATTTGGCAAATGGGTCAGGAGGAGGTCTGGAAGTAATGCCGTGTGGAGGAGATGGGGTTTGGAGAGTGAAGCTTGTGATTGACACTAAGCAGTTAGAGGAGATATTGTCTGAAGGCAACACTCTGGCTCTGATAGAGATGATGAGGATGGCTGCAACAGCAAGTGCTACACCGAAGCTGCAGAGTAAGAGCCTATGGGGTTCAGGAGGAGGTGGGGGTTGGAATTGGAAGCCTATCTTCTCCAACATGTTTAAGGCCCCACGGCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

225

Amino Acids

23.83

Weight (kDa)

4.78

Isoelectric Point (pI)

47.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 17 - 197 1.3e-11 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 214
AccB7I CCANNNNNTGG 1 cut(s) 185
AccBSI CCGCTC 1 cut(s) 12
AciI CCGC 1 cut(s) 10
AcoI YGGCCR 2 cut(s) 247, 671
AcsI RAATTY 1 cut(s) 409
AcuI CTGAAG 1 cut(s) 540
AfiI CCNNNNNNNGG 2 cut(s) 185, 455
AflIII ACRYGT 1 cut(s) 654
AgsI TTSAA 1 cut(s) 320
AhlI ACTAGT 1 cut(s) 242
AjnI CCWGG 1 cut(s) 178
AluBI AGCT 4 cut(s) 155, 362, 481, 586
AluI AGCT 4 cut(s) 155, 362, 481, 586
Alw26I GTCTC 1 cut(s) 310
AlwNI CAGNNNCTG 1 cut(s) 119
AoxI GGCC 5 cut(s) 207, 234, 247, 663, 671
ApeKI GCWGC 2 cut(s) 560, 586
ApoI RAATTY 1 cut(s) 409
AspS9I GGNCC 3 cut(s) 106, 143, 664
AsuHPI GGTGA 1 cut(s) 382
AvaII GGWCC 2 cut(s) 106, 143
AxyI CCTNAGG 1 cut(s) 225
BalI TGGCCA 1 cut(s) 249
BanI GGYRCC 1 cut(s) 214
BbvI GCAGC 2 cut(s) 547, 573
BccI CCATC 3 cut(s) 341, 455, 550
BceAI ACGGC 1 cut(s) 433
BciT130I CCWGG 1 cut(s) 180
BciVI GTATCC 1 cut(s) 137
BcoDI GTCTC 1 cut(s) 310
BcuI ACTAGT 1 cut(s) 242
BfaI CTAG 1 cut(s) 243
BfmI CTRYAG 3 cut(s) 131, 309, 587
BfuI GTATCC 1 cut(s) 137
BisI GCNGC 2 cut(s) 561, 587
BlpI GCTNAGC 1 cut(s) 363
BlsI GCNGC 2 cut(s) 562, 588
Bme1390I CCNGG 1 cut(s) 180
Bme18I GGWCC 2 cut(s) 106, 143
BmgT120I GGNCC 3 cut(s) 106, 143, 664
BmiI GGNNCC 4 cut(s) 76, 107, 216, 666
BmrFI CCNGG 1 cut(s) 180
BmsI GCATC 1 cut(s) 401
Bpu1102I GCTNAGC 1 cut(s) 363
BsaI GGTCTC 1 cut(s) 310
BsaJI CCNNGG 2 cut(s) 210, 668
BsaXI ACNNNNNCTCC 4 cut(s) 450, 480, 500, 530
Bsc4I CCNNNNNNNGG 2 cut(s) 185, 455
Bse118I RCCGGY 1 cut(s) 367
Bse1I ACTGG 1 cut(s) 250
Bse21I CCTNAGG 1 cut(s) 225
Bse3DI GCAATG 1 cut(s) 218
BseBI CCWGG 1 cut(s) 180
BseDI CCNNGG 2 cut(s) 210, 668
BseGI GGATG 2 cut(s) 352, 561
BseLI CCNNNNNNNGG 2 cut(s) 185, 455
BseMI GCAATG 1 cut(s) 218
BseMII CTCAG 3 cut(s) 216, 260, 354
BseNI ACTGG 1 cut(s) 250
BseRI GAGGAG 4 cut(s) 444, 471, 521, 630
BseXI GCAGC 2 cut(s) 547, 573
BsgI GTGCAG 1 cut(s) 372
Bsh1285I CGRYCG 1 cut(s) 10
BshFI GGCC 5 cut(s) 209, 236, 249, 665, 673
BshNI GGYRCC 1 cut(s) 214
BsiEI CGRYCG 1 cut(s) 10
BsiSI CCGG 1 cut(s) 368
BslI CCNNNNNNNGG 2 cut(s) 185, 455
BsmAI GTCTC 1 cut(s) 310
BsnI GGCC 5 cut(s) 209, 236, 249, 665, 673
Bso31I GGTCTC 1 cut(s) 310
Bsp143I GATC 1 cut(s) 3
Bsp1720I GCTNAGC 1 cut(s) 363
BspACI CCGC 1 cut(s) 10
BspANI GGCC 5 cut(s) 209, 236, 249, 665, 673
BspCNI CTCAG 3 cut(s) 217, 261, 355
BspLI GGNNCC 4 cut(s) 76, 107, 216, 666
BspMAI CTGCAG 1 cut(s) 591
BspQI GCTCTTC 1 cut(s) 67
BspT107I GGYRCC 1 cut(s) 214
BspTNI GGTCTC 1 cut(s) 310
BsrBI CCGCTC 1 cut(s) 12
BsrDI GCAATG 1 cut(s) 218
BsrFI RCCGGY 1 cut(s) 367
BsrI ACTGG 1 cut(s) 250
BssAI RCCGGY 1 cut(s) 367
BssECI CCNNGG 2 cut(s) 210, 668
BssMI GATC 1 cut(s) 3
Bst2UI CCWGG 1 cut(s) 180
Bst4CI ACNGT 1 cut(s) 135
Bst6I CTCTTC 2 cut(s) 67, 225
BstC8I GCNNGC 1 cut(s) 291
BstDEI CTNAG 5 cut(s) 147, 225, 269, 363, 495
BstDSI CCRYGG 1 cut(s) 668
BstF5I GGATG 2 cut(s) 352, 561
BstKTI GATC 1 cut(s) 6
BstMAI GTCTC 1 cut(s) 310
BstMBI GATC 1 cut(s) 3
BstMCI CGRYCG 1 cut(s) 10
BstMWI GCNNNNNNNGC 2 cut(s) 359, 566
BstNI CCWGG 1 cut(s) 180
BstNSI RCATGY 1 cut(s) 658
BstSCI CCNGG 1 cut(s) 178
BstSFI CTRYAG 3 cut(s) 131, 309, 587
BstV1I GCAGC 2 cut(s) 547, 573
Bsu36I CCTNAGG 1 cut(s) 225
BsuI GTATCC 1 cut(s) 137
BsuRI GGCC 5 cut(s) 209, 236, 249, 665, 673
BtgI CCRYGG 1 cut(s) 668
BtsCI GGATG 2 cut(s) 352, 561
BtsIMutI CAGTG 1 cut(s) 266
Cac8I GCNNGC 1 cut(s) 291
CaiI CAGNNNCTG 1 cut(s) 119
Cfr10I RCCGGY 1 cut(s) 367
Cfr13I GGNCC 3 cut(s) 106, 143, 664
CviAII CATG 1 cut(s) 655
DdeI CTNAG 5 cut(s) 147, 225, 269, 363, 495
DpnI GATC 1 cut(s) 5
DpnII GATC 1 cut(s) 3
DraI TTTAAA 1 cut(s) 139
EaeI YGGCCR 2 cut(s) 247, 671
Eam1104I CTCTTC 2 cut(s) 67, 225
EarI CTCTTC 2 cut(s) 67, 225
Eco147I AGGCCT 2 cut(s) 209, 236
Eco31I GGTCTC 1 cut(s) 310
Eco47I GGWCC 2 cut(s) 106, 143
Eco57I CTGAAG 1 cut(s) 540
Eco81I CCTNAGG 1 cut(s) 225
EcoO109I RGGNCCY 3 cut(s) 106, 143, 664
EcoRII CCWGG 1 cut(s) 178
FaeI CATG 1 cut(s) 658
FaiI YATR 5 cut(s) 186, 300, 604, 656, 676
FalI AAGNNNNNCTT 2 cut(s) 629, 661
FatI CATG 1 cut(s) 654
Fnu4HI GCNGC 2 cut(s) 561, 587
FokI GGATG 2 cut(s) 359, 568
Fsp4HI GCNGC 2 cut(s) 561, 587
FspBI CTAG 1 cut(s) 243
GluI GCNGC 2 cut(s) 561, 587
HaeIII GGCC 5 cut(s) 209, 236, 249, 665, 673
HapII CCGG 1 cut(s) 368
Hin1II CATG 1 cut(s) 658
HindIII AAGCTT 1 cut(s) 479
HinfI GANTC 1 cut(s) 116
HpaII CCGG 1 cut(s) 368
HphI GGTGA 1 cut(s) 382
Hpy166II GTNNAC 1 cut(s) 266
Hpy188I TCNGA 5 cut(s) 16, 160, 397, 520, 540
Hpy188III TCNNGA 4 cut(s) 320, 426, 437, 612
Hpy8I GTNNAC 1 cut(s) 266
HpyAV CCTTC 2 cut(s) 269, 515
HpyCH4III ACNGT 1 cut(s) 135
HpyCH4V TGCA 4 cut(s) 353, 392, 563, 589
HpyF10VI GCNNNNNNNGC 2 cut(s) 359, 566
HpyF3I CTNAG 5 cut(s) 147, 225, 269, 363, 495
Hsp92II CATG 1 cut(s) 658
Kzo9I GATC 1 cut(s) 3
LguI GCTCTTC 1 cut(s) 67
LmnI GCTCC 1 cut(s) 17
Lsp1109I GCAGC 2 cut(s) 547, 573
LweI GCATC 1 cut(s) 401
MaeI CTAG 1 cut(s) 243
MaeIII GTNAC 1 cut(s) 92
MalI GATC 1 cut(s) 5
MbiI CCGCTC 1 cut(s) 12
MboI GATC 1 cut(s) 3
MboII GAAGA 6 cut(s) 54, 242, 350, 368, 385, 637
MlsI TGGCCA 1 cut(s) 249
MluCI AATT 3 cut(s) 55, 409, 631
MluNI TGGCCA 1 cut(s) 249
MmeI TCCRAC 2 cut(s) 608, 675
Mox20I TGGCCA 1 cut(s) 249
MscI TGGCCA 1 cut(s) 249
MseI TTAA 3 cut(s) 138, 239, 660
Msp20I TGGCCA 1 cut(s) 249
MspI CCGG 1 cut(s) 368
MspR9I CCNGG 1 cut(s) 180
MvaI CCWGG 1 cut(s) 180
MwoI GCNNNNNNNGC 2 cut(s) 359, 566
NdeII GATC 1 cut(s) 3
NlaIII CATG 1 cut(s) 658
NlaIV GGNNCC 4 cut(s) 76, 107, 216, 666
NspI RCATGY 1 cut(s) 658
PceI AGGCCT 2 cut(s) 209, 236
PciI ACATGT 1 cut(s) 654
PciSI GCTCTTC 1 cut(s) 67
PcsI WCGNNNNNNNCGW 1 cut(s) 12
PfeI GAWTC 1 cut(s) 116
PflMI CCANNNNNTGG 1 cut(s) 185
PkrI GCNGC 2 cut(s) 562, 588
PpuMI RGGWCCY 2 cut(s) 106, 143
PscI ACATGT 1 cut(s) 654
Psp5II RGGWCCY 2 cut(s) 106, 143
Psp6I CCWGG 1 cut(s) 178
PspGI CCWGG 1 cut(s) 178
PspN4I GGNNCC 4 cut(s) 76, 107, 216, 666
PspPI GGNCC 3 cut(s) 106, 143, 664
PspPPI RGGWCCY 2 cut(s) 106, 143
PstI CTGCAG 1 cut(s) 591
PstNI CAGNNNCTG 1 cut(s) 119
SapI GCTCTTC 1 cut(s) 67
SaqAI TTAA 3 cut(s) 138, 239, 660
SatI GCNGC 2 cut(s) 561, 587
Sau3AI GATC 1 cut(s) 3
Sau96I GGNCC 3 cut(s) 106, 143, 664
ScrFI CCNGG 1 cut(s) 180
SfaNI GCATC 1 cut(s) 401
SfcI CTRYAG 3 cut(s) 131, 309, 587
SinI GGWCC 2 cut(s) 106, 143
SpeI ACTAGT 1 cut(s) 242
Sse9I AATT 3 cut(s) 55, 409, 631
SseBI AGGCCT 2 cut(s) 209, 236
SsiI CCGC 1 cut(s) 10
SspI AATATT 1 cut(s) 42
SspMI CTAG 1 cut(s) 243
StuI AGGCCT 2 cut(s) 209, 236
StyD4I CCNGG 1 cut(s) 178
TaaI ACNGT 1 cut(s) 135
TaqI TCGA 1 cut(s) 6
TasI AATT 3 cut(s) 55, 409, 631
TfiI GAWTC 1 cut(s) 116
Tru1I TTAA 3 cut(s) 138, 239, 660
Tru9I TTAA 3 cut(s) 138, 239, 660
TscAI CASTG 1 cut(s) 273
TseI GCWGC 2 cut(s) 560, 586
TspDTI ATGAA 1 cut(s) 351
TspRI CASTG 1 cut(s) 273
Van91I CCANNNNNTGG 1 cut(s) 185
VpaK11BI GGWCC 2 cut(s) 106, 143
XapI RAATTY 1 cut(s) 409
XceI RCATGY 1 cut(s) 658
XspI CTAG 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.