Rroxscaffold_3G00261540

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0010034
Physical Location & Seq
Reverse (-)
55796399 .. 55797448
1050 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00261540.1

Sequence Viewer

Length: 699 bp
ATGATCGACCGCTTTGATTGTGGTCAAGGTGGGAAAGAAATATTCACAATGGGAAATTGCTCTTCTCTCAAAGGAACCTGTGGGGAGTGTCCTAACTCTATCAGGGTCCTTACAGATTCTGGGGGTATCCTACAGTTTAAAGGTCCTAAGTTAGCTTCGGAAATCCTCGCTGGTTTTCCAGGCTATGGCATTTTTCAGCAGGGCAAGGCCACGATGCCATTGCCTGAGGAAGAGGCCTTAACTAGTGGCCAGTTCTACCTTCTTCCACTGAGGACTAAAGAGGAAGTGCCTGCTGATAATGGGGTCTCTGTAGAAGTTCAAGAAATGGGTGCTGATGAAGATTTGGAGGGTGCAGAAGAAGCTGAGCAGGTGAAGAAGTCGTTCGGGGGTGCATCGGATTATGTGGAGAATTTGGCAAATGGGTCAGGAGGAGGTCTGGAAGTAATGCCGTGTGGAGGAGATGGGGTTTGGAGAGTGAAGCTTGTGATTGACACTAAGCAGTTGGAGGAGATATTGTCTGAAGGCAACACCCTGGCTCTGATCGAGATGATGAGGATGGCTGCAACAGAGAGTGCTACACCGAAGCTGCAGAGTAAGAGCCTATGGGGTTCAGGAGGAGGTGCGGGTTGGAATTGGAAGCCTATCTTCTCCAACATGTTTAAGGCCCCCACGGCCATAACTGATCACAGTGGGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

232

Amino Acids

24.63

Weight (kDa)

4.56

Isoelectric Point (pI)

44.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 17 - 197 1.2e-12 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 358
Acc36I ACCTGC 1 cut(s) 358
AccB7I CCANNNNNTGG 1 cut(s) 185
AciI CCGC 2 cut(s) 10, 623
AcoI YGGCCR 2 cut(s) 247, 672
AcsI RAATTY 1 cut(s) 409
AcuI CTGAAG 1 cut(s) 540
AfiI CCNNNNNNNGG 2 cut(s) 185, 455
AflIII ACRYGT 1 cut(s) 654
AgsI TTSAA 1 cut(s) 320
AhlI ACTAGT 1 cut(s) 242
AjnI CCWGG 2 cut(s) 178, 531
AluBI AGCT 4 cut(s) 155, 362, 481, 586
AluI AGCT 4 cut(s) 155, 362, 481, 586
Alw26I GTCTC 1 cut(s) 310
AlwNI CAGNNNCTG 1 cut(s) 119
AoxI GGCC 5 cut(s) 207, 234, 247, 663, 672
ApeKI GCWGC 2 cut(s) 560, 586
ApoI RAATTY 1 cut(s) 409
Asp700I GAANNNNTTC 1 cut(s) 380
AspS9I GGNCC 3 cut(s) 106, 143, 664
AsuHPI GGTGA 1 cut(s) 382
AvaII GGWCC 2 cut(s) 106, 143
AxyI CCTNAGG 1 cut(s) 225
BalI TGGCCA 1 cut(s) 249
BbvI GCAGC 2 cut(s) 547, 573
BccI CCATC 2 cut(s) 455, 550
BceAI ACGGC 2 cut(s) 433, 687
BcgI CGANNNNNNTGC 2 cut(s) 202, 236
BciT130I CCWGG 2 cut(s) 180, 533
BciVI GTATCC 1 cut(s) 137
BclI TGATCA 1 cut(s) 682
BcoDI GTCTC 1 cut(s) 310
BcuI ACTAGT 1 cut(s) 242
BfaI CTAG 1 cut(s) 243
BfmI CTRYAG 3 cut(s) 131, 309, 587
BfuAI ACCTGC 1 cut(s) 358
BfuI GTATCC 1 cut(s) 137
BglI GCCNNNNNGGC 1 cut(s) 671
BisI GCNGC 2 cut(s) 561, 587
BlpI GCTNAGC 1 cut(s) 363
BlsI GCNGC 2 cut(s) 562, 588
Bme1390I CCNGG 2 cut(s) 180, 533
Bme18I GGWCC 2 cut(s) 106, 143
BmgT120I GGNCC 3 cut(s) 106, 143, 664
BmiI GGNNCC 3 cut(s) 76, 107, 666
BmrFI CCNGG 2 cut(s) 180, 533
BmsI GCATC 2 cut(s) 204, 401
Bpu1102I GCTNAGC 1 cut(s) 363
BsaI GGTCTC 1 cut(s) 310
BsaJI CCNNGG 2 cut(s) 531, 669
BsaXI ACNNNNNCTCC 4 cut(s) 450, 480, 500, 530
Bsc4I CCNNNNNNNGG 2 cut(s) 185, 455
Bse1I ACTGG 1 cut(s) 250
Bse21I CCTNAGG 1 cut(s) 225
Bse3DI GCAATG 1 cut(s) 218
BseBI CCWGG 2 cut(s) 180, 533
BseDI CCNNGG 2 cut(s) 531, 669
BseGI GGATG 2 cut(s) 561, 699
BseLI CCNNNNNNNGG 2 cut(s) 185, 455
BseMI GCAATG 1 cut(s) 218
BseMII CTCAG 3 cut(s) 216, 260, 354
BseNI ACTGG 1 cut(s) 250
BseRI GAGGAG 4 cut(s) 444, 471, 521, 630
BseXI GCAGC 2 cut(s) 547, 573
BsgI GTGCAG 1 cut(s) 372
Bsh1285I CGRYCG 1 cut(s) 10
BshFI GGCC 5 cut(s) 209, 236, 249, 665, 674
BsiEI CGRYCG 1 cut(s) 10
BslI CCNNNNNNNGG 2 cut(s) 185, 455
BsmAI GTCTC 1 cut(s) 310
BsnI GGCC 5 cut(s) 209, 236, 249, 665, 674
Bso31I GGTCTC 1 cut(s) 310
Bsp143I GATC 3 cut(s) 3, 540, 682
Bsp1720I GCTNAGC 1 cut(s) 363
BspACI CCGC 2 cut(s) 10, 623
BspANI GGCC 5 cut(s) 209, 236, 249, 665, 674
BspCNI CTCAG 3 cut(s) 217, 261, 355
BspLI GGNNCC 3 cut(s) 76, 107, 666
BspMAI CTGCAG 1 cut(s) 591
BspMI ACCTGC 1 cut(s) 358
BspQI GCTCTTC 1 cut(s) 67
BspTNI GGTCTC 1 cut(s) 310
BsrDI GCAATG 1 cut(s) 218
BsrI ACTGG 1 cut(s) 250
BssECI CCNNGG 2 cut(s) 531, 669
BssMI GATC 3 cut(s) 3, 540, 682
Bst2UI CCWGG 2 cut(s) 180, 533
Bst4CI ACNGT 2 cut(s) 135, 689
Bst6I CTCTTC 2 cut(s) 67, 225
BstC8I GCNNGC 1 cut(s) 291
BstDEI CTNAG 5 cut(s) 147, 225, 269, 363, 495
BstDSI CCRYGG 1 cut(s) 669
BstF5I GGATG 2 cut(s) 561, 699
BstKTI GATC 3 cut(s) 6, 543, 685
BstMAI GTCTC 1 cut(s) 310
BstMBI GATC 3 cut(s) 3, 540, 682
BstMCI CGRYCG 1 cut(s) 10
BstMWI GCNNNNNNNGC 2 cut(s) 359, 671
BstNI CCWGG 2 cut(s) 180, 533
BstNSI RCATGY 1 cut(s) 658
BstSCI CCNGG 2 cut(s) 178, 531
BstSFI CTRYAG 3 cut(s) 131, 309, 587
BstV1I GCAGC 2 cut(s) 547, 573
Bsu36I CCTNAGG 1 cut(s) 225
BsuI GTATCC 1 cut(s) 137
BsuRI GGCC 5 cut(s) 209, 236, 249, 665, 674
BtgI CCRYGG 1 cut(s) 669
BtsCI GGATG 2 cut(s) 561, 699
BtsIMutI CAGTG 2 cut(s) 266, 694
BveI ACCTGC 1 cut(s) 358
Cac8I GCNNGC 1 cut(s) 291
CaiI CAGNNNCTG 1 cut(s) 119
Cfr13I GGNCC 3 cut(s) 106, 143, 664
CviAII CATG 1 cut(s) 655
DdeI CTNAG 5 cut(s) 147, 225, 269, 363, 495
DpnI GATC 3 cut(s) 5, 542, 684
DpnII GATC 3 cut(s) 3, 540, 682
DraI TTTAAA 1 cut(s) 139
EaeI YGGCCR 2 cut(s) 247, 672
Eam1104I CTCTTC 2 cut(s) 67, 225
EarI CTCTTC 2 cut(s) 67, 225
Eco147I AGGCCT 1 cut(s) 236
Eco31I GGTCTC 1 cut(s) 310
Eco47I GGWCC 2 cut(s) 106, 143
Eco57I CTGAAG 1 cut(s) 540
Eco81I CCTNAGG 1 cut(s) 225
EcoO109I RGGNCCY 3 cut(s) 106, 143, 664
EcoRII CCWGG 2 cut(s) 178, 531
FaeI CATG 1 cut(s) 658
FaiI YATR 5 cut(s) 186, 402, 604, 656, 677
FalI AAGNNNNNCTT 2 cut(s) 629, 661
FatI CATG 1 cut(s) 654
FauI CCCGC 1 cut(s) 616
FbaI TGATCA 1 cut(s) 682
Fnu4HI GCNGC 2 cut(s) 561, 587
FokI GGATG 1 cut(s) 568
Fsp4HI GCNGC 2 cut(s) 561, 587
FspBI CTAG 1 cut(s) 243
GluI GCNGC 2 cut(s) 561, 587
HaeIII GGCC 5 cut(s) 209, 236, 249, 665, 674
Hin1II CATG 1 cut(s) 658
HindIII AAGCTT 1 cut(s) 479
HinfI GANTC 1 cut(s) 116
HphI GGTGA 1 cut(s) 382
Hpy188I TCNGA 4 cut(s) 160, 397, 520, 540
Hpy188III TCNNGA 5 cut(s) 320, 426, 437, 544, 612
HpyAV CCTTC 2 cut(s) 269, 515
HpyCH4III ACNGT 2 cut(s) 135, 689
HpyCH4V TGCA 4 cut(s) 353, 392, 563, 589
HpyF10VI GCNNNNNNNGC 2 cut(s) 359, 671
HpyF3I CTNAG 5 cut(s) 147, 225, 269, 363, 495
Hsp92II CATG 1 cut(s) 658
Ksp22I TGATCA 1 cut(s) 682
Kzo9I GATC 3 cut(s) 3, 540, 682
LguI GCTCTTC 1 cut(s) 67
Lsp1109I GCAGC 2 cut(s) 547, 573
LweI GCATC 2 cut(s) 204, 401
MaeI CTAG 1 cut(s) 243
MalI GATC 3 cut(s) 5, 542, 684
MboI GATC 3 cut(s) 3, 540, 682
MboII GAAGA 7 cut(s) 54, 242, 254, 350, 368, 385, 637
MlsI TGGCCA 1 cut(s) 249
MluCI AATT 3 cut(s) 55, 409, 631
MluNI TGGCCA 1 cut(s) 249
MmeI TCCRAC 3 cut(s) 483, 608, 675
Mox20I TGGCCA 1 cut(s) 249
MroXI GAANNNNTTC 1 cut(s) 380
MscI TGGCCA 1 cut(s) 249
MseI TTAA 3 cut(s) 138, 239, 660
Msp20I TGGCCA 1 cut(s) 249
MspR9I CCNGG 2 cut(s) 180, 533
MvaI CCWGG 2 cut(s) 180, 533
MwoI GCNNNNNNNGC 2 cut(s) 359, 671
NdeII GATC 3 cut(s) 3, 540, 682
NlaIII CATG 1 cut(s) 658
NlaIV GGNNCC 3 cut(s) 76, 107, 666
NspI RCATGY 1 cut(s) 658
PaqCI CACCTGC 1 cut(s) 358
PceI AGGCCT 1 cut(s) 236
PciI ACATGT 1 cut(s) 654
PciSI GCTCTTC 1 cut(s) 67
PdmI GAANNNNTTC 1 cut(s) 380
PfeI GAWTC 1 cut(s) 116
PflMI CCANNNNNTGG 1 cut(s) 185
PkrI GCNGC 2 cut(s) 562, 588
PpuMI RGGWCCY 2 cut(s) 106, 143
PscI ACATGT 1 cut(s) 654
Psp5II RGGWCCY 2 cut(s) 106, 143
Psp6I CCWGG 2 cut(s) 178, 531
PspGI CCWGG 2 cut(s) 178, 531
PspN4I GGNNCC 3 cut(s) 76, 107, 666
PspPI GGNCC 3 cut(s) 106, 143, 664
PspPPI RGGWCCY 2 cut(s) 106, 143
PstI CTGCAG 1 cut(s) 591
PstNI CAGNNNCTG 1 cut(s) 119
SapI GCTCTTC 1 cut(s) 67
SaqAI TTAA 3 cut(s) 138, 239, 660
SatI GCNGC 2 cut(s) 561, 587
Sau3AI GATC 3 cut(s) 3, 540, 682
Sau96I GGNCC 3 cut(s) 106, 143, 664
ScrFI CCNGG 2 cut(s) 180, 533
SfaNI GCATC 2 cut(s) 204, 401
SfcI CTRYAG 3 cut(s) 131, 309, 587
SfiI GGCCNNNNNGGCC 1 cut(s) 671
SinI GGWCC 2 cut(s) 106, 143
SpeI ACTAGT 1 cut(s) 242
Sse9I AATT 3 cut(s) 55, 409, 631
SseBI AGGCCT 1 cut(s) 236
SsiI CCGC 2 cut(s) 10, 623
SspI AATATT 1 cut(s) 42
SspMI CTAG 1 cut(s) 243
StuI AGGCCT 1 cut(s) 236
StyD4I CCNGG 2 cut(s) 178, 531
TaaI ACNGT 2 cut(s) 135, 689
TaqI TCGA 2 cut(s) 6, 543
TasI AATT 3 cut(s) 55, 409, 631
TfiI GAWTC 1 cut(s) 116
Tru1I TTAA 3 cut(s) 138, 239, 660
Tru9I TTAA 3 cut(s) 138, 239, 660
TscAI CASTG 2 cut(s) 273, 694
TseI GCWGC 2 cut(s) 560, 586
TspDTI ATGAA 1 cut(s) 351
TspRI CASTG 2 cut(s) 273, 694
Van91I CCANNNNNTGG 1 cut(s) 185
VpaK11BI GGWCC 2 cut(s) 106, 143
XapI RAATTY 1 cut(s) 409
XceI RCATGY 1 cut(s) 658
XmnI GAANNNNTTC 1 cut(s) 380
XspI CTAG 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.