FvH4_5g05340

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007928
Physical Location & Seq
Forward (+)
3123031 .. 3124593
1563 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g05340.t1

Sequence Viewer

Length: 582 bp
ATGAGGATCGTTGCAGTTGCCAGATTGTTTCATGCCCGTCCCACACTGCCTAAAGCAACTGGTCATTTGCAGGTTCGTGCGTTCCAACCGGATTTCAAGCCCAGGGATCCCAAGGCCAAGCCGATACGCTATAAATATCCGGAAGTTTATAACCCTTATGGTCCTAGACCTCCACCCTCGGATAAGATTGTTCAGCTTGCTGAAAGAATTGCGGCACTGGCCCCTGCAGAACGGAGCCAACTTGGTCCTGCACTTGTGGAGAGGCTAAAACTTCCGCAGATGCAGCACATATCAACAGAAGGCATGGACTTAGGTGCACAGGGAGGGGCTGCTGGTGCGTCGAAGGCTGAGGAGAAGAAGGTTGAGAAAACTGCTTTTGATGTCAAGTTGGAGAAGTTTGAAGCATCCGCAAAAATCAAGGTGATCAAGGAAGTGCGGGCTTTTACCAGTCTCGGATTGAAGGAGGCTAAAGATCTTGTTGAGAAGGCACCTTGTGTGCTCAAACAGGGGCTTACCAAAGAGGAAGCAAACGACATGATTGAGAAATTAAAGGCTGCAGGAGGAGTTGCTGTCATGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L12

Protein Analysis

194

Amino Acids

21.15

Weight (kDa)

9.64

Isoelectric Point (pI)

26.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L12 PF00542 126 - 189 1e-22 Ribosomal protein L7/L12 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 150
Acc36I ACCTGC 1 cut(s) 61
AccB1I GGYRCC 1 cut(s) 487
AccIII TCCGGA 1 cut(s) 139
AciI CCGC 4 cut(s) 212, 275, 408, 436
AclWI GGATC 3 cut(s) 14, 101, 114
AdeI CACNNNGTG 1 cut(s) 494
AgsI TTSAA 3 cut(s) 97, 401, 460
AjnI CCWGG 1 cut(s) 101
AluBI AGCT 1 cut(s) 196
AluI AGCT 1 cut(s) 196
Alw21I GWGCWC 2 cut(s) 319, 501
Alw26I GTCTC 1 cut(s) 455
Alw44I GTGCAC 1 cut(s) 315
AlwI GGATC 3 cut(s) 14, 101, 114
Aor13HI TCCGGA 1 cut(s) 139
AoxI GGCC 2 cut(s) 114, 219
ApaLI GTGCAC 1 cut(s) 315
ApeKI GCWGC 3 cut(s) 283, 329, 554
AspS9I GGNCC 3 cut(s) 161, 220, 245
AsuHPI GGTGA 1 cut(s) 433
AvaII GGWCC 2 cut(s) 161, 245
BaeGI GKGCMC 1 cut(s) 319
BamHI GGATCC 1 cut(s) 106
BanI GGYRCC 1 cut(s) 487
Bbv12I GWGCWC 2 cut(s) 319, 501
BbvCI CCTCAGC 1 cut(s) 348
BbvI GCAGC 3 cut(s) 295, 316, 541
BciT130I CCWGG 1 cut(s) 103
BclI TGATCA 1 cut(s) 423
BcoDI GTCTC 1 cut(s) 455
BfaI CTAG 1 cut(s) 165
BfmI CTRYAG 2 cut(s) 225, 555
BfuAI ACCTGC 1 cut(s) 61
BglII AGATCT 1 cut(s) 472
BisI GCNGC 4 cut(s) 213, 284, 330, 555
BlsI GCNGC 4 cut(s) 214, 285, 331, 556
Bme1390I CCNGG 1 cut(s) 103
Bme18I GGWCC 2 cut(s) 161, 245
BmgT120I GGNCC 3 cut(s) 161, 220, 245
BmiI GGNNCC 4 cut(s) 108, 222, 236, 489
BmrFI CCNGG 1 cut(s) 103
BmsI GCATC 2 cut(s) 270, 413
Bpu10I CCTNAGC 1 cut(s) 348
BsaJI CCNNGG 4 cut(s) 101, 102, 111, 177
BsaWI WCCGGW 2 cut(s) 88, 139
BsaXI ACNNNNNCTCC 1 cut(s) 555
Bse1I ACTGG 3 cut(s) 64, 222, 447
BseAI TCCGGA 1 cut(s) 139
BseBI CCWGG 1 cut(s) 103
BseDI CCNNGG 4 cut(s) 101, 102, 111, 177
BseGI GGATG 1 cut(s) 404
BseMII CTCAG 1 cut(s) 339
BseNI ACTGG 3 cut(s) 64, 222, 447
BseRI GAGGAG 2 cut(s) 365, 576
BseSI GKGCMC 1 cut(s) 319
BseXI GCAGC 3 cut(s) 295, 316, 541
BsgI GTGCAG 1 cut(s) 234
BshFI GGCC 2 cut(s) 116, 221
BshNI GGYRCC 1 cut(s) 487
BsiHKAI GWGCWC 2 cut(s) 319, 501
BsiSI CCGG 2 cut(s) 89, 140
BslFI GGGAC 1 cut(s) 24
BsmAI GTCTC 1 cut(s) 455
BsmFI GGGAC 1 cut(s) 24
BsnI GGCC 2 cut(s) 116, 221
Bsp1286I GDGCHC 2 cut(s) 319, 501
Bsp13I TCCGGA 1 cut(s) 139
Bsp143I GATC 4 cut(s) 6, 106, 423, 472
BspACI CCGC 4 cut(s) 212, 275, 408, 436
BspANI GGCC 2 cut(s) 116, 221
BspCNI CTCAG 1 cut(s) 340
BspEI TCCGGA 1 cut(s) 139
BspLI GGNNCC 4 cut(s) 108, 222, 236, 489
BspMAI CTGCAG 2 cut(s) 229, 559
BspMI ACCTGC 1 cut(s) 61
BspPI GGATC 3 cut(s) 14, 101, 114
BspT107I GGYRCC 1 cut(s) 487
BsrI ACTGG 3 cut(s) 64, 222, 447
BssECI CCNNGG 4 cut(s) 101, 102, 111, 177
BssMI GATC 4 cut(s) 6, 106, 423, 472
BssT1I CCWWGG 1 cut(s) 111
Bst2UI CCWGG 1 cut(s) 103
BstC8I GCNNGC 2 cut(s) 198, 438
BstDEI CTNAG 2 cut(s) 310, 348
BstF5I GGATG 1 cut(s) 404
BstKTI GATC 4 cut(s) 9, 109, 426, 475
BstMAI GTCTC 1 cut(s) 455
BstMBI GATC 4 cut(s) 6, 106, 423, 472
BstMWI GCNNNNNNNGC 4 cut(s) 218, 283, 335, 344
BstNI CCWGG 1 cut(s) 103
BstSCI CCNGG 1 cut(s) 101
BstSFI CTRYAG 2 cut(s) 225, 555
BstSLI GKGCMC 1 cut(s) 319
BstV1I GCAGC 3 cut(s) 295, 316, 541
BstX2I RGATCY 2 cut(s) 106, 472
BstYI RGATCY 2 cut(s) 106, 472
BsuRI GGCC 2 cut(s) 116, 221
BtsCI GGATG 1 cut(s) 404
BtsI GCAGTG 1 cut(s) 44
BtsIMutI CAGTG 2 cut(s) 44, 215
BveI ACCTGC 1 cut(s) 61
Cac8I GCNNGC 2 cut(s) 198, 438
Cfr13I GGNCC 3 cut(s) 161, 220, 245
CseI GACGC 1 cut(s) 327
CviAII CATG 4 cut(s) 32, 304, 535, 574
DdeI CTNAG 2 cut(s) 310, 348
DpnI GATC 4 cut(s) 8, 108, 425, 474
DpnII GATC 4 cut(s) 6, 106, 423, 472
DraIII CACNNNGTG 1 cut(s) 494
Eco130I CCWWGG 1 cut(s) 111
Eco47I GGWCC 2 cut(s) 161, 245
EcoRII CCWGG 1 cut(s) 101
EcoT14I CCWWGG 1 cut(s) 111
ErhI CCWWGG 1 cut(s) 111
FaeI CATG 4 cut(s) 35, 307, 538, 577
FaiI YATR 8 cut(s) 33, 132, 150, 159, 290, 305, 536, 575
FaqI GGGAC 1 cut(s) 24
FatI CATG 4 cut(s) 31, 303, 534, 573
FauI CCCGC 1 cut(s) 429
FbaI TGATCA 1 cut(s) 423
Fnu4HI GCNGC 4 cut(s) 213, 284, 330, 555
FokI GGATG 1 cut(s) 391
Fsp4HI GCNGC 4 cut(s) 213, 284, 330, 555
FspBI CTAG 1 cut(s) 165
GluI GCNGC 4 cut(s) 213, 284, 330, 555
HaeIII GGCC 2 cut(s) 116, 221
HapII CCGG 2 cut(s) 89, 140
HgaI GACGC 1 cut(s) 327
Hin1II CATG 4 cut(s) 35, 307, 538, 577
HpaII CCGG 2 cut(s) 89, 140
HphI GGTGA 1 cut(s) 433
Hpy166II GTNNAC 1 cut(s) 317
Hpy188I TCNGA 2 cut(s) 181, 455
Hpy188III TCNNGA 1 cut(s) 140
Hpy8I GTNNAC 1 cut(s) 317
Hpy99I CGWCG 1 cut(s) 343
HpyAV CCTTC 5 cut(s) 293, 337, 352, 454, 478
HpyCH4V TGCA 7 cut(s) 14, 70, 227, 251, 283, 317, 557
HpyF10VI GCNNNNNNNGC 4 cut(s) 218, 283, 335, 344
HpyF3I CTNAG 2 cut(s) 310, 348
Hsp92II CATG 4 cut(s) 35, 307, 538, 577
Kpn2I TCCGGA 1 cut(s) 139
Ksp22I TGATCA 1 cut(s) 423
Kzo9I GATC 4 cut(s) 6, 106, 423, 472
LmnI GCTCC 1 cut(s) 234
Lsp1109I GCAGC 3 cut(s) 295, 316, 541
LweI GCATC 2 cut(s) 270, 413
MaeI CTAG 1 cut(s) 165
MalI GATC 4 cut(s) 8, 108, 425, 474
MboI GATC 4 cut(s) 6, 106, 423, 472
MboII GAAGA 1 cut(s) 367
MflI RGATCY 2 cut(s) 106, 472
MhlI GDGCHC 2 cut(s) 319, 501
MluCI AATT 2 cut(s) 207, 545
MmeI TCCRAC 2 cut(s) 109, 369
MnlI CCTC 8 cut(s) 180, 187, 255, 317, 343, 457, 514, 554
MroI TCCGGA 1 cut(s) 139
MseI TTAA 1 cut(s) 548
MspI CCGG 2 cut(s) 89, 140
MspR9I CCNGG 1 cut(s) 103
MvaI CCWGG 1 cut(s) 103
MwoI GCNNNNNNNGC 4 cut(s) 218, 283, 335, 344
NdeII GATC 4 cut(s) 6, 106, 423, 472
NlaIII CATG 4 cut(s) 35, 307, 538, 577
NlaIV GGNNCC 4 cut(s) 108, 222, 236, 489
PasI CCCWGGG 1 cut(s) 102
PkrI GCNGC 4 cut(s) 214, 285, 331, 556
PsiI TTATAA 1 cut(s) 150
Psp6I CCWGG 1 cut(s) 101
PspGI CCWGG 1 cut(s) 101
PspN4I GGNNCC 4 cut(s) 108, 222, 236, 489
PspPI GGNCC 3 cut(s) 161, 220, 245
PstI CTGCAG 2 cut(s) 229, 559
PsuI RGATCY 2 cut(s) 106, 472
SaqAI TTAA 1 cut(s) 548
SatI GCNGC 4 cut(s) 213, 284, 330, 555
Sau3AI GATC 4 cut(s) 6, 106, 423, 472
Sau96I GGNCC 3 cut(s) 161, 220, 245
ScrFI CCNGG 1 cut(s) 103
SduI GDGCHC 2 cut(s) 319, 501
SetI ASST 7 cut(s) 75, 172, 198, 316, 363, 423, 493
SfaNI GCATC 2 cut(s) 270, 413
SfcI CTRYAG 2 cut(s) 225, 555
SinI GGWCC 2 cut(s) 161, 245
Sse9I AATT 2 cut(s) 207, 545
SsiI CCGC 4 cut(s) 212, 275, 408, 436
SspMI CTAG 1 cut(s) 165
StyD4I CCNGG 1 cut(s) 101
StyI CCWWGG 1 cut(s) 111
TaqI TCGA 1 cut(s) 341
TasI AATT 2 cut(s) 207, 545
TauI GCSGC 1 cut(s) 215
Tru1I TTAA 1 cut(s) 548
Tru9I TTAA 1 cut(s) 548
TscAI CASTG 2 cut(s) 51, 222
TseI GCWGC 3 cut(s) 283, 329, 554
TspDTI ATGAA 1 cut(s) 20
TspGWI ACGGA 1 cut(s) 247
TspRI CASTG 2 cut(s) 51, 222
VneI GTGCAC 1 cut(s) 315
VpaK11BI GGWCC 2 cut(s) 161, 245
XspI CTAG 1 cut(s) 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.