MD06G1173500.v1.1

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0007928
Physical Location & Seq
Reverse (-)
31332066 .. 31333682
1617 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1173500.v1.1.491

Sequence Viewer

Length: 582 bp
ATGAGGCTCATTGCGCTTGCTAGATCTTTTCGTGCGCGTCCTACACTCCCTGAAACAACTGGTCCTTTGCAGTTTCGTTGCTTTCAACCTGATTTTGTGCCCCGGGATCCCAGTGCCAAGCCAAAGAAGTACAAATATCCTGCATTCTATGATCCGTATGGCCCGAGACCTCCACCTTCAGATAAAATCGTTCAGCTTGCTGAGCGAATCGCAGCACTGGCCCCTGAAGAACGCCGCCAACTTGGTCCTACTCTTGCTGAAAGGCTAAGGCATCCGAAGCTGCAGCCCATATCAACAGAAGGCATGGACTTGGGTTCACAGGGAGGGGCAGCTGCTGGGTCAAAGGCTGAGGAAAAGAAGGTGGAGAAGACGGCTTTTGATGTGAAATTGGAGAAGTTCGATGCTGCTGCAAAAATCAAGGTGATCAAGGAAGTCAGGACTTTTACCAGTCTGGGATTGAAGGAGGCTAAAGATCTCGTCGAGAAGGTACCTTGTGTGCTTAAACAAGGGGTTACCAAAGAGGAAGCAAATGACATAATTGAGAAAATAAAGGCTGCTGGAGGAGTTGCTGTTATGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L12

Protein Analysis

194

Amino Acids

21.28

Weight (kDa)

9.52

Isoelectric Point (pI)

41.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L12 PF00542 126 - 189 3.2e-22 Ribosomal protein L7/L12 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 487
AccB1I GGYRCC 1 cut(s) 487
AccII CGCG 1 cut(s) 37
AciI CCGC 1 cut(s) 235
AclWI GGATC 3 cut(s) 101, 114, 146
AcuI CTGAAG 2 cut(s) 162, 246
AfaI GTAC 2 cut(s) 131, 489
AgsI TTSAA 2 cut(s) 86, 460
AluBI AGCT 3 cut(s) 196, 280, 332
AluI AGCT 3 cut(s) 196, 280, 332
Alw26I GTCTC 1 cut(s) 160
AlwI GGATC 3 cut(s) 101, 114, 146
AlwNI CAGNNNCTG 1 cut(s) 335
Ama87I CYCGRG 2 cut(s) 102, 163
AoxI GGCC 2 cut(s) 160, 219
ApeKI GCWGC 8 cut(s) 212, 280, 283, 329, 332, 404, 407, 554
Asp718I GGTACC 1 cut(s) 487
AspLEI GCGC 2 cut(s) 16, 37
AspS9I GGNCC 4 cut(s) 62, 161, 220, 245
AsuC2I CCSGG 2 cut(s) 103, 104
AsuHPI GGTGA 1 cut(s) 433
AvaI CYCGRG 2 cut(s) 102, 163
AvaII GGWCC 2 cut(s) 62, 245
BaeGI GKGCMC 1 cut(s) 102
BamHI GGATCC 1 cut(s) 106
BanI GGYRCC 1 cut(s) 487
BbsI GAAGAC 1 cut(s) 374
BbvCI CCTCAGC 1 cut(s) 348
BbvI GCAGC 8 cut(s) 224, 267, 295, 319, 341, 391, 394, 541
BceAI ACGGC 1 cut(s) 387
BcgI CGANNNNNNTGC 2 cut(s) 389, 423
BclI TGATCA 1 cut(s) 423
BcnI CCSGG 2 cut(s) 103, 104
BcoDI GTCTC 1 cut(s) 160
BfaI CTAG 1 cut(s) 21
BfmI CTRYAG 1 cut(s) 281
BglII AGATCT 2 cut(s) 23, 472
BisI GCNGC 9 cut(s) 213, 235, 281, 284, 330, 333, 405, 408, 555
BlpI GCTNAGC 1 cut(s) 201
BlsI GCNGC 9 cut(s) 214, 236, 282, 285, 331, 334, 406, 409, 556
Bme1390I CCNGG 2 cut(s) 103, 104
Bme18I GGWCC 2 cut(s) 62, 245
BmeT110I CYCGRG 2 cut(s) 102, 163
BmgT120I GGNCC 4 cut(s) 62, 161, 220, 245
BmiI GGNNCC 3 cut(s) 108, 222, 489
BmrFI CCNGG 2 cut(s) 103, 104
BmrI ACTGGG 1 cut(s) 105
BmsI GCATC 2 cut(s) 280, 391
BmuI ACTGGG 1 cut(s) 105
BpiI GAAGAC 1 cut(s) 374
BpmI CTGGAG 1 cut(s) 579
Bpu10I CCTNAGC 2 cut(s) 266, 348
Bpu1102I GCTNAGC 1 cut(s) 201
BpuMI CCSGG 2 cut(s) 103, 104
BsaI GGTCTC 1 cut(s) 160
BsaJI CCNNGG 2 cut(s) 101, 102
BsaXI ACNNNNNCTCC 1 cut(s) 555
Bse1I ACTGG 4 cut(s) 64, 111, 222, 447
Bse3DI GCAATG 1 cut(s) 9
BseDI CCNNGG 2 cut(s) 101, 102
BseGI GGATG 1 cut(s) 271
BseMI GCAATG 1 cut(s) 9
BseMII CTCAG 2 cut(s) 192, 339
BseNI ACTGG 4 cut(s) 64, 111, 222, 447
BseRI GAGGAG 1 cut(s) 576
BseSI GKGCMC 1 cut(s) 102
BseXI GCAGC 8 cut(s) 224, 267, 295, 319, 341, 391, 394, 541
BseYI CCCAGC 1 cut(s) 335
Bsh1236I CGCG 1 cut(s) 37
BshFI GGCC 2 cut(s) 162, 221
BshNI GGYRCC 1 cut(s) 487
BsiHKCI CYCGRG 2 cut(s) 102, 163
BsiSI CCGG 1 cut(s) 103
BsmAI GTCTC 1 cut(s) 160
BsmI GAATGC 1 cut(s) 143
BsnI GGCC 2 cut(s) 162, 221
Bso31I GGTCTC 1 cut(s) 160
BsoBI CYCGRG 2 cut(s) 102, 163
Bsp1286I GDGCHC 1 cut(s) 102
Bsp143I GATC 5 cut(s) 23, 106, 151, 423, 472
Bsp1720I GCTNAGC 1 cut(s) 201
BspACI CCGC 1 cut(s) 235
BspANI GGCC 2 cut(s) 162, 221
BspCNI CTCAG 2 cut(s) 193, 340
BspFNI CGCG 1 cut(s) 37
BspLI GGNNCC 3 cut(s) 108, 222, 489
BspMAI CTGCAG 1 cut(s) 285
BspPI GGATC 3 cut(s) 101, 114, 146
BspT107I GGYRCC 1 cut(s) 487
BspTNI GGTCTC 1 cut(s) 160
BsrDI GCAATG 1 cut(s) 9
BsrI ACTGG 4 cut(s) 64, 111, 222, 447
BssECI CCNNGG 2 cut(s) 101, 102
BssMI GATC 5 cut(s) 23, 106, 151, 423, 472
BstC8I GCNNGC 2 cut(s) 18, 198
BstDEI CTNAG 3 cut(s) 201, 266, 348
BstEII GGTNACC 1 cut(s) 511
BstF5I GGATG 1 cut(s) 271
BstFNI CGCG 1 cut(s) 37
BstHHI GCGC 2 cut(s) 16, 37
BstKTI GATC 5 cut(s) 26, 109, 154, 426, 475
BstMAI GTCTC 1 cut(s) 160
BstMBI GATC 5 cut(s) 23, 106, 151, 423, 472
BstMWI GCNNNNNNNGC 4 cut(s) 13, 202, 218, 277
BstPI GGTNACC 1 cut(s) 511
BstSCI CCNGG 2 cut(s) 101, 102
BstSFI CTRYAG 1 cut(s) 281
BstSLI GKGCMC 1 cut(s) 102
BstUI CGCG 1 cut(s) 37
BstV1I GCAGC 8 cut(s) 224, 267, 295, 319, 341, 391, 394, 541
BstV2I GAAGAC 1 cut(s) 374
BstX2I RGATCY 3 cut(s) 23, 106, 472
BstYI RGATCY 3 cut(s) 23, 106, 472
BsuRI GGCC 2 cut(s) 162, 221
BtsCI GGATG 1 cut(s) 271
BtsIMutI CAGTG 2 cut(s) 118, 215
Cac8I GCNNGC 2 cut(s) 18, 198
CaiI CAGNNNCTG 1 cut(s) 335
CfoI GCGC 2 cut(s) 16, 37
Cfr13I GGNCC 4 cut(s) 62, 161, 220, 245
Cfr9I CCCGGG 1 cut(s) 102
CseI GACGC 1 cut(s) 26
Csp6I GTAC 2 cut(s) 130, 488
CviAII CATG 1 cut(s) 304
CviQI GTAC 2 cut(s) 130, 488
DdeI CTNAG 3 cut(s) 201, 266, 348
DpnI GATC 5 cut(s) 25, 108, 153, 425, 474
DpnII GATC 5 cut(s) 23, 106, 151, 423, 472
Eco31I GGTCTC 1 cut(s) 160
Eco47I GGWCC 2 cut(s) 62, 245
Eco57I CTGAAG 2 cut(s) 162, 246
Eco88I CYCGRG 2 cut(s) 102, 163
Eco91I GGTNACC 1 cut(s) 511
EcoO65I GGTNACC 1 cut(s) 511
FaeI CATG 1 cut(s) 307
FaiI YATR 6 cut(s) 150, 159, 290, 305, 536, 575
FatI CATG 1 cut(s) 303
FbaI TGATCA 1 cut(s) 423
Fnu4HI GCNGC 9 cut(s) 213, 235, 281, 284, 330, 333, 405, 408, 555
FokI GGATG 1 cut(s) 258
Fsp4HI GCNGC 9 cut(s) 213, 235, 281, 284, 330, 333, 405, 408, 555
FspBI CTAG 1 cut(s) 21
GlaI GCGC 2 cut(s) 15, 36
GluI GCNGC 9 cut(s) 213, 235, 281, 284, 330, 333, 405, 408, 555
GsaI CCCAGC 1 cut(s) 339
GsuI CTGGAG 1 cut(s) 579
HaeIII GGCC 2 cut(s) 162, 221
HapII CCGG 1 cut(s) 103
HgaI GACGC 1 cut(s) 26
HhaI GCGC 2 cut(s) 16, 37
Hin1II CATG 1 cut(s) 307
Hin6I GCGC 2 cut(s) 14, 35
HinP1I GCGC 2 cut(s) 14, 35
HinfI GANTC 1 cut(s) 207
HpaII CCGG 1 cut(s) 103
HphI GGTGA 1 cut(s) 433
Hpy166II GTNNAC 1 cut(s) 317
Hpy188I TCNGA 2 cut(s) 181, 276
Hpy188III TCNNGA 2 cut(s) 436, 481
Hpy8I GTNNAC 1 cut(s) 317
Hpy99I CGWCG 1 cut(s) 482
HpyAV CCTTC 5 cut(s) 186, 293, 352, 454, 478
HpyCH4V TGCA 4 cut(s) 70, 143, 283, 410
HpyF10VI GCNNNNNNNGC 4 cut(s) 13, 202, 218, 277
HpyF3I CTNAG 3 cut(s) 201, 266, 348
Hsp92II CATG 1 cut(s) 307
HspAI GCGC 2 cut(s) 14, 35
KpnI GGTACC 1 cut(s) 491
Ksp22I TGATCA 1 cut(s) 423
Kzo9I GATC 5 cut(s) 23, 106, 151, 423, 472
Lsp1109I GCAGC 8 cut(s) 224, 267, 295, 319, 341, 391, 394, 541
LweI GCATC 2 cut(s) 280, 391
MaeI CTAG 1 cut(s) 21
MaeIII GTNAC 1 cut(s) 511
MalI GATC 5 cut(s) 25, 108, 153, 425, 474
MboI GATC 5 cut(s) 23, 106, 151, 423, 472
MboII GAAGA 2 cut(s) 239, 379
MflI RGATCY 3 cut(s) 23, 106, 472
MhlI GDGCHC 1 cut(s) 102
MluCI AATT 2 cut(s) 386, 537
MnlI CCTC 6 cut(s) 180, 317, 343, 457, 514, 554
MseI TTAA 1 cut(s) 501
MspA1I CMGCKG 1 cut(s) 332
MspI CCGG 1 cut(s) 103
MspR9I CCNGG 2 cut(s) 103, 104
Mva1269I GAATGC 1 cut(s) 143
MvnI CGCG 1 cut(s) 37
MwoI GCNNNNNNNGC 4 cut(s) 13, 202, 218, 277
NciI CCSGG 2 cut(s) 103, 104
NdeII GATC 5 cut(s) 23, 106, 151, 423, 472
NlaIII CATG 1 cut(s) 307
NlaIV GGNNCC 3 cut(s) 108, 222, 489
PctI GAATGC 1 cut(s) 143
PfeI GAWTC 1 cut(s) 207
PkrI GCNGC 9 cut(s) 214, 236, 282, 285, 331, 334, 406, 409, 556
PspEI GGTNACC 1 cut(s) 511
PspFI CCCAGC 1 cut(s) 335
PspN4I GGNNCC 3 cut(s) 108, 222, 489
PspPI GGNCC 4 cut(s) 62, 161, 220, 245
PstI CTGCAG 1 cut(s) 285
PstNI CAGNNNCTG 1 cut(s) 335
PsuI RGATCY 3 cut(s) 23, 106, 472
PvuII CAGCTG 1 cut(s) 332
RsaI GTAC 2 cut(s) 131, 489
RsaNI GTAC 2 cut(s) 130, 488
SaqAI TTAA 1 cut(s) 501
SatI GCNGC 9 cut(s) 213, 235, 281, 284, 330, 333, 405, 408, 555
Sau3AI GATC 5 cut(s) 23, 106, 151, 423, 472
Sau96I GGNCC 4 cut(s) 62, 161, 220, 245
ScrFI CCNGG 2 cut(s) 103, 104
SduI GDGCHC 1 cut(s) 102
SfaNI GCATC 2 cut(s) 280, 391
SfcI CTRYAG 1 cut(s) 281
SinI GGWCC 2 cut(s) 62, 245
SmaI CCCGGG 1 cut(s) 104
Sse9I AATT 2 cut(s) 386, 537
SsiI CCGC 1 cut(s) 235
SspMI CTAG 1 cut(s) 21
StyD4I CCNGG 2 cut(s) 101, 102
TaqI TCGA 2 cut(s) 399, 480
TasI AATT 2 cut(s) 386, 537
TatI WGTACW 1 cut(s) 129
TauI GCSGC 1 cut(s) 237
TfiI GAWTC 1 cut(s) 207
Tru1I TTAA 1 cut(s) 501
Tru9I TTAA 1 cut(s) 501
TscAI CASTG 2 cut(s) 118, 222
TseI GCWGC 8 cut(s) 212, 280, 283, 329, 332, 404, 407, 554
TspGWI ACGGA 1 cut(s) 144
TspMI CCCGGG 1 cut(s) 102
TspRI CASTG 2 cut(s) 118, 222
VpaK11BI GGWCC 2 cut(s) 62, 245
XmaI CCCGGG 1 cut(s) 102
XspI CTAG 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.