RLG00000004266

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0007928
Physical Location & Seq
Forward (+)
57829356 .. 57830584
1229 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004266

Sequence Viewer

Length: 681 bp
ATGGTCATCCTCTCGTCTCTGCTCTCTTTCCCTCATTTCGCTCTGTTGGATTCGGCGGCTGTTATCCCCCTCATTTATAAACCCACGACGATAGAGTTCTCAACATTGGTCGAGCAGTTCAAATCTTTTCGTGCTCGTCCCACACTGCCTAAAGCAACCGGTCATTTGCAAGTTCGTGCGTTCCAACCCGATTTTGTTCCAAGGGATCCCAAGGCCAAGCCGATACGATACAAATATCCGGAAGTCTATAACCCCTATGGCCCTAGACCCCCACCCTCGGATAAAATTGTTCAGCTTGCTGAAAGAATTGCGGCTCTGGCCCCTGCAGAACGGTGTCAACTTGGTCCTGCACTCGTGGAGAAGCTAAAGCTTCCTCAGCTGCAGCCCATATCAACAGAAGGCATGGACTTAGGTCCACAGGGAGGGGCTGCTGGTGGGTCAAAGGCTGAGGAGAAGAAGGTTGAGAAAACTGCTTTTGATGTCAAATTGGAGAAGTTTGAATCGTCTGCAAAAATCAAGGTGATCAAGGAAGTGAGGGCTTTTACCAGTCTCGGATTGAAGGAGGCTAAAGAACTTGTTGAGAAGGCACCTTGTGTGCTTAAACAAGGGCTTACCAAAGAGGAAGCAAATGACATGATTGAGAAACTAAAGGCTGCTGGAGGAGTTGCTGTTATGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L12

Protein Analysis

227

Amino Acids

24.75

Weight (kDa)

9.27

Isoelectric Point (pI)

31.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L12 PF00542 159 - 222 3.2e-22 Ribosomal protein L7/L12 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 78
AccB1I GGYRCC 1 cut(s) 586
AccIII TCCGGA 1 cut(s) 238
AciI CCGC 2 cut(s) 56, 311
AclWI GGATC 2 cut(s) 200, 213
AdeI CACNNNGTG 1 cut(s) 593
AfiI CCNNNNNNNGG 2 cut(s) 277, 422
AgeI ACCGGT 1 cut(s) 158
AgsI TTSAA 3 cut(s) 121, 500, 559
AluBI AGCT 4 cut(s) 295, 364, 370, 379
AluI AGCT 4 cut(s) 295, 364, 370, 379
Alw21I GWGCWC 1 cut(s) 136
Alw26I GTCTC 2 cut(s) 21, 554
AlwI GGATC 2 cut(s) 200, 213
Aor13HI TCCGGA 1 cut(s) 238
AoxI GGCC 3 cut(s) 213, 259, 318
ApeKI GCWGC 4 cut(s) 379, 382, 428, 653
AsiGI ACCGGT 1 cut(s) 158
AspS9I GGNCC 4 cut(s) 260, 319, 344, 413
AsuHPI GGTGA 1 cut(s) 532
AvaII GGWCC 2 cut(s) 344, 413
BamHI GGATCC 1 cut(s) 205
BanI GGYRCC 1 cut(s) 586
BauI CACGAG 1 cut(s) 353
Bbv12I GWGCWC 1 cut(s) 136
BbvCI CCTCAGC 2 cut(s) 375, 447
BbvI GCAGC 4 cut(s) 366, 394, 415, 640
BclI TGATCA 1 cut(s) 522
BcoDI GTCTC 2 cut(s) 21, 554
BfaI CTAG 1 cut(s) 264
BfmI CTRYAG 2 cut(s) 324, 380
BisI GCNGC 6 cut(s) 57, 312, 380, 383, 429, 654
BlsI GCNGC 6 cut(s) 58, 313, 381, 384, 430, 655
Bme18I GGWCC 2 cut(s) 344, 413
BmgT120I GGNCC 4 cut(s) 260, 319, 344, 413
BmiI GGNNCC 3 cut(s) 207, 321, 588
BoxI GACNNNNGTC 1 cut(s) 411
BpmI CTGGAG 1 cut(s) 678
Bpu10I CCTNAGC 2 cut(s) 375, 447
BsaJI CCNNGG 3 cut(s) 200, 210, 276
BsaWI WCCGGW 2 cut(s) 158, 238
BsaXI ACNNNNNCTCC 1 cut(s) 654
Bsc4I CCNNNNNNNGG 2 cut(s) 277, 422
Bse118I RCCGGY 1 cut(s) 158
Bse1I ACTGG 1 cut(s) 546
BseAI TCCGGA 1 cut(s) 238
BseDI CCNNGG 3 cut(s) 200, 210, 276
BseGI GGATG 1 cut(s) 6
BseLI CCNNNNNNNGG 2 cut(s) 277, 422
BseMII CTCAG 2 cut(s) 389, 438
BseNI ACTGG 1 cut(s) 546
BseRI GAGGAG 2 cut(s) 464, 675
BseXI GCAGC 4 cut(s) 366, 394, 415, 640
BsgI GTGCAG 1 cut(s) 333
BshFI GGCC 3 cut(s) 215, 261, 320
BshNI GGYRCC 1 cut(s) 586
BshTI ACCGGT 1 cut(s) 158
BsiHKAI GWGCWC 1 cut(s) 136
BsiSI CCGG 2 cut(s) 159, 239
BslFI GGGAC 1 cut(s) 123
BslI CCNNNNNNNGG 2 cut(s) 277, 422
BsmAI GTCTC 2 cut(s) 21, 554
BsmBI CGTCTC 1 cut(s) 21
BsmFI GGGAC 1 cut(s) 123
BsnI GGCC 3 cut(s) 215, 261, 320
Bsp1286I GDGCHC 1 cut(s) 136
Bsp13I TCCGGA 1 cut(s) 238
Bsp143I GATC 2 cut(s) 205, 522
BspACI CCGC 2 cut(s) 56, 311
BspANI GGCC 3 cut(s) 215, 261, 320
BspCNI CTCAG 2 cut(s) 388, 439
BspEI TCCGGA 1 cut(s) 238
BspLI GGNNCC 3 cut(s) 207, 321, 588
BspMAI CTGCAG 2 cut(s) 328, 384
BspPI GGATC 2 cut(s) 200, 213
BspT107I GGYRCC 1 cut(s) 586
BsrFI RCCGGY 1 cut(s) 158
BsrI ACTGG 1 cut(s) 546
BssAI RCCGGY 1 cut(s) 158
BssECI CCNNGG 3 cut(s) 200, 210, 276
BssMI GATC 2 cut(s) 205, 522
BssSI CACGAG 1 cut(s) 353
BssT1I CCWWGG 2 cut(s) 200, 210
Bst2BI CACGAG 1 cut(s) 353
Bst4CI ACNGT 1 cut(s) 333
BstC8I GCNNGC 1 cut(s) 297
BstDEI CTNAG 3 cut(s) 375, 409, 447
BstF5I GGATG 1 cut(s) 6
BstKTI GATC 2 cut(s) 208, 525
BstMAI GTCTC 2 cut(s) 21, 554
BstMBI GATC 2 cut(s) 205, 522
BstMWI GCNNNNNNNGC 2 cut(s) 317, 376
BstPAI GACNNNNGTC 1 cut(s) 411
BstSFI CTRYAG 2 cut(s) 324, 380
BstV1I GCAGC 4 cut(s) 366, 394, 415, 640
BstX2I RGATCY 1 cut(s) 205
BstYI RGATCY 1 cut(s) 205
BsuRI GGCC 3 cut(s) 215, 261, 320
BtsCI GGATG 1 cut(s) 6
BtsI GCAGTG 1 cut(s) 143
BtsIMutI CAGTG 1 cut(s) 143
Cac8I GCNNGC 1 cut(s) 297
Cfr10I RCCGGY 1 cut(s) 158
Cfr13I GGNCC 4 cut(s) 260, 319, 344, 413
CspAI ACCGGT 1 cut(s) 158
CviAII CATG 2 cut(s) 403, 634
DdeI CTNAG 3 cut(s) 375, 409, 447
DpnI GATC 2 cut(s) 207, 524
DpnII GATC 2 cut(s) 205, 522
DraIII CACNNNGTG 1 cut(s) 593
Eco130I CCWWGG 2 cut(s) 200, 210
Eco47I GGWCC 2 cut(s) 344, 413
EcoT14I CCWWGG 2 cut(s) 200, 210
ErhI CCWWGG 2 cut(s) 200, 210
Esp3I CGTCTC 1 cut(s) 21
FaeI CATG 2 cut(s) 406, 637
FaiI YATR 7 cut(s) 78, 249, 258, 389, 404, 635, 674
FaqI GGGAC 1 cut(s) 123
FatI CATG 2 cut(s) 402, 633
FbaI TGATCA 1 cut(s) 522
Fnu4HI GCNGC 6 cut(s) 57, 312, 380, 383, 429, 654
Fsp4HI GCNGC 6 cut(s) 57, 312, 380, 383, 429, 654
FspBI CTAG 1 cut(s) 264
GluI GCNGC 6 cut(s) 57, 312, 380, 383, 429, 654
GsuI CTGGAG 1 cut(s) 678
HaeIII GGCC 3 cut(s) 215, 261, 320
HapII CCGG 2 cut(s) 159, 239
Hin1II CATG 2 cut(s) 406, 637
HincII GTYRAC 1 cut(s) 338
HindII GTYRAC 1 cut(s) 338
HindIII AAGCTT 1 cut(s) 368
HinfI GANTC 2 cut(s) 50, 500
HpaII CCGG 2 cut(s) 159, 239
HphI GGTGA 1 cut(s) 532
Hpy166II GTNNAC 2 cut(s) 338, 416
Hpy188I TCNGA 2 cut(s) 280, 554
Hpy188III TCNNGA 1 cut(s) 239
Hpy8I GTNNAC 2 cut(s) 338, 416
Hpy99I CGWCG 1 cut(s) 91
HpyAV CCTTC 4 cut(s) 392, 451, 553, 577
HpyCH4III ACNGT 1 cut(s) 333
HpyCH4V TGCA 5 cut(s) 169, 326, 350, 382, 509
HpyF10VI GCNNNNNNNGC 2 cut(s) 317, 376
HpyF3I CTNAG 3 cut(s) 375, 409, 447
Hsp92II CATG 2 cut(s) 406, 637
Kpn2I TCCGGA 1 cut(s) 238
Ksp22I TGATCA 1 cut(s) 522
Kzo9I GATC 2 cut(s) 205, 522
LpnPI CCDG 9 cut(s) 172, 252, 302, 336, 360, 404, 417, 559, 642
Lsp1109I GCAGC 4 cut(s) 366, 394, 415, 640
MaeI CTAG 1 cut(s) 264
MalI GATC 2 cut(s) 207, 524
MboI GATC 2 cut(s) 205, 522
MboII GAAGA 1 cut(s) 466
MflI RGATCY 1 cut(s) 205
MhlI GDGCHC 1 cut(s) 136
MluCI AATT 3 cut(s) 285, 306, 485
MmeI TCCRAC 2 cut(s) 27, 208
MroI TCCGGA 1 cut(s) 238
MseI TTAA 1 cut(s) 600
MspA1I CMGCKG 1 cut(s) 379
MspI CCGG 2 cut(s) 159, 239
MwoI GCNNNNNNNGC 2 cut(s) 317, 376
NdeII GATC 2 cut(s) 205, 522
NlaIII CATG 2 cut(s) 406, 637
NlaIV GGNNCC 3 cut(s) 207, 321, 588
PfeI GAWTC 2 cut(s) 50, 500
PinAI ACCGGT 1 cut(s) 158
PkrI GCNGC 6 cut(s) 58, 313, 381, 384, 430, 655
PshAI GACNNNNGTC 1 cut(s) 411
PsiI TTATAA 1 cut(s) 78
PspN4I GGNNCC 3 cut(s) 207, 321, 588
PspPI GGNCC 4 cut(s) 260, 319, 344, 413
PstI CTGCAG 2 cut(s) 328, 384
PsuI RGATCY 1 cut(s) 205
PvuII CAGCTG 1 cut(s) 379
SaqAI TTAA 1 cut(s) 600
SatI GCNGC 6 cut(s) 57, 312, 380, 383, 429, 654
Sau3AI GATC 2 cut(s) 205, 522
Sau96I GGNCC 4 cut(s) 260, 319, 344, 413
SduI GDGCHC 1 cut(s) 136
SetI ASST 8 cut(s) 297, 366, 372, 381, 415, 462, 522, 592
SfcI CTRYAG 2 cut(s) 324, 380
SinI GGWCC 2 cut(s) 344, 413
Sse9I AATT 3 cut(s) 285, 306, 485
SsiI CCGC 2 cut(s) 56, 311
SspMI CTAG 1 cut(s) 264
StyI CCWWGG 2 cut(s) 200, 210
TaaI ACNGT 1 cut(s) 333
TaqI TCGA 1 cut(s) 111
TasI AATT 3 cut(s) 285, 306, 485
TauI GCSGC 2 cut(s) 59, 314
TfiI GAWTC 2 cut(s) 50, 500
Tru1I TTAA 1 cut(s) 600
Tru9I TTAA 1 cut(s) 600
TscAI CASTG 1 cut(s) 150
TseI GCWGC 4 cut(s) 379, 382, 428, 653
TspRI CASTG 1 cut(s) 150
VpaK11BI GGWCC 2 cut(s) 344, 413
XspI CTAG 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.