MD14G1180000.v1.1

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007928
Physical Location & Seq
Reverse (-)
27262801 .. 27264333
1533 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1180000.v1.1.491

Sequence Viewer

Length: 582 bp
ATGAGGCTCATTGCAGTTGCCAGATCTTTTCGTGCCCGTCCCACTCTCCCTGAAACAACTGGTCCTTTGCAGTTTCGTTGCTTCCAACCTGATTTTGTGCCCCGGGACCCCAATGCCAAGCCAAAAAAGTACAAATATCCGGTATTCTATGACCCGTACGGCCCTAGACCTCCACCTTCAGATAAAATCGTTCAGCTTGCCGAGCGAATTGCAGCACTGGCCACTGAAGAACGGCGCCAGCTTGGTCCTACTCTAGCTGAGAGGCTAAGGCATCCGAAGCTGCAACCAATATCGACAGAAGGCTTGGACTTGGGTTCACAGGGAGGGGCGGCTGGTGGTTCAAAGGCCGAGGAAAAGAAGGTGGAGAAAACGGCTTTTGATGTGAAATTGGAGAAGTTCGATGCCGCTGCAAAAATCAAGGTGATCAAGGAAGTGAGGACATTTACCAGTCTGGGATTGAAGGAGGCTAAAGATCTCGTCGAGAAGGTACCTTGTGTGCTTAAACAAGGGGTTACCAAAGAGGAAGCAAGTGACATAATTGAGAAAATAAAGACGGCTGGAGGAGTTGCCGTCATGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L12

Protein Analysis

194

Amino Acids

21.3

Weight (kDa)

9.52

Isoelectric Point (pI)

40.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L12 PF00542 126 - 189 8.4e-22 Ribosomal protein L7/L12 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 487
AccB1I GGYRCC 2 cut(s) 234, 487
AciI CCGC 2 cut(s) 329, 405
AcoI YGGCCR 1 cut(s) 219
AcuI CTGAAG 2 cut(s) 162, 246
AcyI GRCGYC 1 cut(s) 235
AfaI GTAC 3 cut(s) 131, 158, 489
AgsI TTSAA 2 cut(s) 342, 460
AluBI AGCT 4 cut(s) 196, 241, 257, 280
AluI AGCT 4 cut(s) 196, 241, 257, 280
Ama87I CYCGRG 1 cut(s) 102
AoxI GGCC 3 cut(s) 160, 219, 345
ApeKI GCWGC 3 cut(s) 212, 280, 407
Asp718I GGTACC 1 cut(s) 487
AspLEI GCGC 1 cut(s) 237
AspS9I GGNCC 4 cut(s) 62, 106, 161, 245
AsuC2I CCSGG 2 cut(s) 103, 104
AsuHPI GGTGA 1 cut(s) 433
AvaI CYCGRG 1 cut(s) 102
AvaII GGWCC 3 cut(s) 62, 106, 245
BaeGI GKGCMC 2 cut(s) 37, 102
BalI TGGCCA 1 cut(s) 221
BanI GGYRCC 2 cut(s) 234, 487
BbvI GCAGC 3 cut(s) 224, 267, 394
BceAI ACGGC 5 cut(s) 175, 248, 387, 554, 570
BcgI CGANNNNNNTGC 4 cut(s) 191, 225, 389, 423
BclI TGATCA 1 cut(s) 423
BcnI CCSGG 2 cut(s) 103, 104
BfaI CTAG 2 cut(s) 165, 254
BfoI RGCGCY 1 cut(s) 238
BglII AGATCT 2 cut(s) 23, 472
BisI GCNGC 5 cut(s) 213, 281, 330, 405, 408
BlsI GCNGC 5 cut(s) 214, 282, 331, 406, 409
Bme1390I CCNGG 2 cut(s) 103, 104
Bme18I GGWCC 3 cut(s) 62, 106, 245
BmeT110I CYCGRG 1 cut(s) 102
BmgT120I GGNCC 4 cut(s) 62, 106, 161, 245
BmiI GGNNCC 4 cut(s) 107, 108, 236, 489
BmrFI CCNGG 2 cut(s) 103, 104
BmsI GCATC 2 cut(s) 280, 391
BpmI CTGGAG 1 cut(s) 579
Bpu10I CCTNAGC 1 cut(s) 266
BpuMI CCSGG 2 cut(s) 103, 104
BsaHI GRCGYC 1 cut(s) 235
BsaJI CCNNGG 3 cut(s) 101, 102, 348
BsaWI WCCGGW 1 cut(s) 139
BsaXI ACNNNNNCTCC 1 cut(s) 555
Bse1I ACTGG 3 cut(s) 64, 222, 447
Bse3DI GCAATG 1 cut(s) 9
BseDI CCNNGG 3 cut(s) 101, 102, 348
BseGI GGATG 1 cut(s) 271
BseMI GCAATG 1 cut(s) 9
BseMII CTCAG 1 cut(s) 249
BseNI ACTGG 3 cut(s) 64, 222, 447
BseRI GAGGAG 1 cut(s) 576
BseSI GKGCMC 2 cut(s) 37, 102
BseXI GCAGC 3 cut(s) 224, 267, 394
BshFI GGCC 3 cut(s) 162, 221, 347
BshNI GGYRCC 2 cut(s) 234, 487
BsiHKCI CYCGRG 1 cut(s) 102
BsiSI CCGG 2 cut(s) 103, 140
BsiWI CGTACG 1 cut(s) 156
BslFI GGGAC 2 cut(s) 24, 119
BsmFI GGGAC 2 cut(s) 24, 119
BsnI GGCC 3 cut(s) 162, 221, 347
BsoBI CYCGRG 1 cut(s) 102
Bsp1286I GDGCHC 2 cut(s) 37, 102
Bsp143I GATC 3 cut(s) 23, 423, 472
BspACI CCGC 2 cut(s) 329, 405
BspANI GGCC 3 cut(s) 162, 221, 347
BspCNI CTCAG 1 cut(s) 250
BspLI GGNNCC 4 cut(s) 107, 108, 236, 489
BspT107I GGYRCC 2 cut(s) 234, 487
BsrDI GCAATG 1 cut(s) 9
BsrI ACTGG 3 cut(s) 64, 222, 447
BssECI CCNNGG 3 cut(s) 101, 102, 348
BssMI GATC 3 cut(s) 23, 423, 472
BssNI GRCGYC 1 cut(s) 235
BstACI GRCGYC 1 cut(s) 235
BstC8I GCNNGC 2 cut(s) 198, 239
BstDEI CTNAG 2 cut(s) 258, 266
BstEII GGTNACC 1 cut(s) 511
BstF5I GGATG 1 cut(s) 271
BstH2I RGCGCY 1 cut(s) 238
BstHHI GCGC 1 cut(s) 237
BstKTI GATC 3 cut(s) 26, 426, 475
BstMBI GATC 3 cut(s) 23, 423, 472
BstMWI GCNNNNNNNGC 3 cut(s) 202, 218, 277
BstPI GGTNACC 1 cut(s) 511
BstSCI CCNGG 2 cut(s) 101, 102
BstSLI GKGCMC 2 cut(s) 37, 102
BstV1I GCAGC 3 cut(s) 224, 267, 394
BstX2I RGATCY 2 cut(s) 23, 472
BstYI RGATCY 2 cut(s) 23, 472
BsuRI GGCC 3 cut(s) 162, 221, 347
BtsCI GGATG 1 cut(s) 271
BtsIMutI CAGTG 2 cut(s) 215, 222
Cac8I GCNNGC 2 cut(s) 198, 239
CfoI GCGC 1 cut(s) 237
Cfr13I GGNCC 4 cut(s) 62, 106, 161, 245
Cfr9I CCCGGG 1 cut(s) 102
Csp6I GTAC 3 cut(s) 130, 157, 488
CviAII CATG 1 cut(s) 574
CviQI GTAC 3 cut(s) 130, 157, 488
DdeI CTNAG 2 cut(s) 258, 266
DinI GGCGCC 1 cut(s) 236
DpnI GATC 3 cut(s) 25, 425, 474
DpnII GATC 3 cut(s) 23, 423, 472
EaeI YGGCCR 1 cut(s) 219
Eco47I GGWCC 3 cut(s) 62, 106, 245
Eco57I CTGAAG 2 cut(s) 162, 246
Eco88I CYCGRG 1 cut(s) 102
Eco91I GGTNACC 1 cut(s) 511
EcoO109I RGGNCCY 1 cut(s) 106
EcoO65I GGTNACC 1 cut(s) 511
EgeI GGCGCC 1 cut(s) 236
EheI GGCGCC 1 cut(s) 236
FaeI CATG 1 cut(s) 577
FaiI YATR 3 cut(s) 150, 536, 575
FaqI GGGAC 2 cut(s) 24, 119
FatI CATG 1 cut(s) 573
FbaI TGATCA 1 cut(s) 423
Fnu4HI GCNGC 5 cut(s) 213, 281, 330, 405, 408
FokI GGATG 1 cut(s) 258
Fsp4HI GCNGC 5 cut(s) 213, 281, 330, 405, 408
FspBI CTAG 2 cut(s) 165, 254
GlaI GCGC 1 cut(s) 236
GluI GCNGC 5 cut(s) 213, 281, 330, 405, 408
GsuI CTGGAG 1 cut(s) 579
HaeII RGCGCY 1 cut(s) 238
HaeIII GGCC 3 cut(s) 162, 221, 347
HapII CCGG 2 cut(s) 103, 140
HhaI GCGC 1 cut(s) 237
Hin1I GRCGYC 1 cut(s) 235
Hin1II CATG 1 cut(s) 577
Hin6I GCGC 1 cut(s) 235
HinP1I GCGC 1 cut(s) 235
HpaII CCGG 2 cut(s) 103, 140
HphI GGTGA 1 cut(s) 433
Hpy166II GTNNAC 1 cut(s) 317
Hpy188I TCNGA 2 cut(s) 181, 276
Hpy188III TCNNGA 1 cut(s) 481
Hpy8I GTNNAC 1 cut(s) 317
Hpy99I CGWCG 1 cut(s) 482
HpyAV CCTTC 5 cut(s) 186, 293, 352, 454, 478
HpyCH4V TGCA 5 cut(s) 14, 70, 212, 283, 410
HpyF10VI GCNNNNNNNGC 3 cut(s) 202, 218, 277
HpyF3I CTNAG 2 cut(s) 258, 266
Hsp92I GRCGYC 1 cut(s) 235
Hsp92II CATG 1 cut(s) 577
HspAI GCGC 1 cut(s) 235
KasI GGCGCC 1 cut(s) 234
KflI GGGWCCC 1 cut(s) 106
KpnI GGTACC 1 cut(s) 491
Ksp22I TGATCA 1 cut(s) 423
Kzo9I GATC 3 cut(s) 23, 423, 472
Lsp1109I GCAGC 3 cut(s) 224, 267, 394
LweI GCATC 2 cut(s) 280, 391
MaeI CTAG 2 cut(s) 165, 254
MaeIII GTNAC 2 cut(s) 511, 530
MalI GATC 3 cut(s) 25, 425, 474
MboI GATC 3 cut(s) 23, 423, 472
MboII GAAGA 1 cut(s) 239
MflI RGATCY 2 cut(s) 23, 472
MhlI GDGCHC 2 cut(s) 37, 102
MlsI TGGCCA 1 cut(s) 221
MluCI AATT 3 cut(s) 207, 386, 537
MluNI TGGCCA 1 cut(s) 221
Mly113I GGCGCC 1 cut(s) 235
MmeI TCCRAC 1 cut(s) 109
MnlI CCTC 8 cut(s) 180, 255, 317, 343, 429, 457, 514, 554
Mox20I TGGCCA 1 cut(s) 221
MscI TGGCCA 1 cut(s) 221
MseI TTAA 1 cut(s) 501
Msp20I TGGCCA 1 cut(s) 221
MspA1I CMGCKG 1 cut(s) 407
MspI CCGG 2 cut(s) 103, 140
MspR9I CCNGG 2 cut(s) 103, 104
MwoI GCNNNNNNNGC 3 cut(s) 202, 218, 277
NarI GGCGCC 1 cut(s) 235
NciI CCSGG 2 cut(s) 103, 104
NdeII GATC 3 cut(s) 23, 423, 472
NlaIII CATG 1 cut(s) 577
NlaIV GGNNCC 4 cut(s) 107, 108, 236, 489
NmeAIII GCCGAG 2 cut(s) 226, 373
NmuCI GTSAC 1 cut(s) 530
Pfl23II CGTACG 1 cut(s) 156
PkrI GCNGC 5 cut(s) 214, 282, 331, 406, 409
PluTI GGCGCC 1 cut(s) 238
PpuMI RGGWCCY 1 cut(s) 106
Psp5II RGGWCCY 1 cut(s) 106
PspEI GGTNACC 1 cut(s) 511
PspLI CGTACG 1 cut(s) 156
PspN4I GGNNCC 4 cut(s) 107, 108, 236, 489
PspPI GGNCC 4 cut(s) 62, 106, 161, 245
PspPPI RGGWCCY 1 cut(s) 106
PsuI RGATCY 2 cut(s) 23, 472
RsaI GTAC 3 cut(s) 131, 158, 489
RsaNI GTAC 3 cut(s) 130, 157, 488
SaqAI TTAA 1 cut(s) 501
SatI GCNGC 5 cut(s) 213, 281, 330, 405, 408
Sau3AI GATC 3 cut(s) 23, 423, 472
Sau96I GGNCC 4 cut(s) 62, 106, 161, 245
ScrFI CCNGG 2 cut(s) 103, 104
SduI GDGCHC 2 cut(s) 37, 102
SfaNI GCATC 2 cut(s) 280, 391
SfoI GGCGCC 1 cut(s) 236
SinI GGWCC 3 cut(s) 62, 106, 245
SmaI CCCGGG 1 cut(s) 104
Sse9I AATT 3 cut(s) 207, 386, 537
SsiI CCGC 2 cut(s) 329, 405
SspDI GGCGCC 1 cut(s) 234
SspMI CTAG 2 cut(s) 165, 254
StyD4I CCNGG 2 cut(s) 101, 102
TaqI TCGA 3 cut(s) 293, 399, 480
TasI AATT 3 cut(s) 207, 386, 537
TatI WGTACW 1 cut(s) 129
TauI GCSGC 2 cut(s) 332, 407
Tru1I TTAA 1 cut(s) 501
Tru9I TTAA 1 cut(s) 501
TscAI CASTG 2 cut(s) 222, 229
TseFI GTSAC 1 cut(s) 530
TseI GCWGC 3 cut(s) 212, 280, 407
Tsp45I GTSAC 1 cut(s) 530
TspMI CCCGGG 1 cut(s) 102
TspRI CASTG 2 cut(s) 222, 229
VpaK11BI GGWCC 3 cut(s) 62, 106, 245
XmaI CCCGGG 1 cut(s) 102
XspI CTAG 2 cut(s) 165, 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.