FvH4_5g06650

Proline transporter 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0011559
Physical Location & Seq
Reverse (-)
3934372 .. 3937108
2737 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g06650.t3

Sequence Viewer

Length: 1353 bp
ATGGAAGGACCAGAAGGTAACACAAACAACAATGAAGCTGGTTTAAGCATTGAACAAGCTCGAACAGAGTGTGATCAGGATCATGAATATGTACTCCCCTCAGCTCATACTGTTGGTCATGATTCATGGCAGCAAGTTGGGCTGATGCTGGTGACAGGCTTCAATTGCGGTTACATATTGAGCTTTTCTAATCTGATGCTGGTGCCTTTGGGTTGGACATGGGGTATCATCTGCCTTGTTGTAGTTGGCTTCTATACTGCCTATGCTAACTGGCTTTTGGCTGCTTTTCACTTCATCAATGGCCAAAGATTCATCAGATACAGAGATCTTATGGGCTATTTGTTTGGGAGAGAACTGTTTTACATTACATGGGTTTTCCAGTTCTTGACACTTCTTCTTGGAAATATGGGCTTCATTCTTCTAGGGGGTAGAGCACTCAAGGAGATTAACTCAGAACTGAGTGATTCTCCACTGAGGCTCCAATACTTTATTGTCATCACTGGAGTGACCTACTTCGTATTCGCGTTTTTCATTCCAACAATGTCTAAAATGAGATTTTGGCTTGGACCCTCTACCATTGTCACATTTGCTTATATTGTCATACTCCTGGTGGTACTAGTTAAAGATGGGAAAACTAAGACTACAAAGCGAGATTATGAAATCCATGGGAACAAAGCAGACAGAGTATTCAATGCCTTCAATGCAATTTCAGCTATTATTGTCTGCAACACCTCAGGCTTGCTACCAGAAATACAGTCAACTCTGCGGAAGCCGGCAGTCACAAACATGAGGAAAGGTTTATATCTGCAGTATAGTGCAGGGCTCTTATTTTACTATGGAGTCAGCATAGTAGGGTACTGGGCTTATGGTGCAACCGTATCTGAATACCTTCCTGAAGAGCTAAGTGGTCCTAAATCGGTTAAGGTCCTCATAAACACTGCTGTTTTTCTACAATCCATAGTCTCACAACATATGTTTGTTGCTCCAATTCATGAGGCCCTTGATACCAAGTTCCTCAAACTTGATGAGAGCATGCACTCGAAAAATAATATCAAGCGGAGATTCTACCTCCGAGCCCTTCTCTTTATGGGAAACACATTTGTGACAGCAGCTTTTCCTTTCATGGGGGATTTTGTGAACTTATTTGGATCGTTTACACTTGTTCCTCTAACTTTTGTCTTTCCAAGCATGATTTTCATAAAGGTAAAGGGAAAGAAGGCTAGACTGGAGAAAAATATGTGGCATTGGTTCAACATTGTTATTTTTTCTCTGCTTGCGATTGTAACTACAATTTCTGCAGTTCGTTTGATTGTCAACAATGTCAAGGAATACAATTTCTTTGCAGATACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

451

Amino Acids

51.14

Weight (kDa)

9.12

Isoelectric Point (pI)

31.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 41 - 463 5.2e-55 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 202
AccII CGCG 1 cut(s) 524
AciI CCGC 3 cut(s) 168, 766, 1057
AclWI GGATC 2 cut(s) 87, 1156
AcoI YGGCCR 1 cut(s) 301
AcuI CTGAAG 1 cut(s) 915
AfaI GTAC 3 cut(s) 93, 615, 857
AfiI CCNNNNNNNGG 1 cut(s) 1124
AgsI TTSAA 5 cut(s) 53, 163, 691, 700, 1252
AhlI ACTAGT 1 cut(s) 616
AjnI CCWGG 1 cut(s) 606
AleI CACNNNNGTG 2 cut(s) 503, 1100
AluBI AGCT 7 cut(s) 38, 59, 104, 183, 713, 901, 1112
AluI AGCT 7 cut(s) 38, 59, 104, 183, 713, 901, 1112
Alw21I GWGCWC 1 cut(s) 436
Alw26I GTCTC 1 cut(s) 967
AlwI GGATC 2 cut(s) 87, 1156
AoxI GGCC 2 cut(s) 301, 996
ApeKI GCWGC 3 cut(s) 130, 281, 1109
Asp700I GAANNNNTTC 1 cut(s) 888
AspS9I GGNCC 5 cut(s) 8, 566, 908, 925, 997
AsuHPI GGTGA 1 cut(s) 163
AvaII GGWCC 4 cut(s) 8, 566, 908, 925
AxyI CCTNAGG 1 cut(s) 733
BalI TGGCCA 1 cut(s) 303
BanI GGYRCC 1 cut(s) 202
BanII GRGCYC 2 cut(s) 825, 1078
Bbv12I GWGCWC 1 cut(s) 436
BbvCI CCTCAGC 1 cut(s) 100
BbvI GCAGC 3 cut(s) 142, 268, 1121
BccI CCATC 1 cut(s) 620
BciT130I CCWGG 1 cut(s) 608
BclI TGATCA 1 cut(s) 73
BcoDI GTCTC 1 cut(s) 967
BcuI ACTAGT 1 cut(s) 616
BfaI CTAG 3 cut(s) 422, 617, 1221
BfmI CTRYAG 2 cut(s) 806, 1296
BglII AGATCT 1 cut(s) 325
BisI GCNGC 3 cut(s) 131, 282, 1110
BlsI GCNGC 3 cut(s) 132, 283, 1111
Bme1390I CCNGG 1 cut(s) 608
Bme18I GGWCC 4 cut(s) 8, 566, 908, 925
BmgT120I GGNCC 5 cut(s) 8, 566, 908, 925, 997
BmiI GGNNCC 3 cut(s) 204, 479, 568
BmrFI CCNGG 1 cut(s) 608
BmrI ACTGGG 1 cut(s) 868
BmsI GCATC 2 cut(s) 135, 186
BmuI ACTGGG 1 cut(s) 868
BplI GAGNNNNNCTC 6 cut(s) 434, 466, 451, 483, 1065, 1097
BpmI CTGGAG 2 cut(s) 522, 1247
Bpu10I CCTNAGC 1 cut(s) 100
BpuEI CTTGAG 1 cut(s) 422
BsaBI GATNNNNATC 1 cut(s) 78
BsaJI CCNNGG 1 cut(s) 664
BsaXI ACNNNNNCTCC 2 cut(s) 462, 492
Bsc4I CCNNNNNNNGG 1 cut(s) 1124
Bse118I RCCGGY 1 cut(s) 772
Bse1I ACTGG 5 cut(s) 275, 379, 505, 863, 1230
Bse21I CCTNAGG 1 cut(s) 733
Bse8I GATNNNNATC 1 cut(s) 78
BseBI CCWGG 1 cut(s) 608
BseDI CCNNGG 1 cut(s) 664
BseJI GATNNNNATC 1 cut(s) 78
BseLI CCNNNNNNNGG 1 cut(s) 1124
BseMII CTCAG 5 cut(s) 114, 449, 464, 465, 747
BseNI ACTGG 5 cut(s) 275, 379, 505, 863, 1230
BseXI GCAGC 3 cut(s) 142, 268, 1121
BsgI GTGCAG 1 cut(s) 837
Bsh1236I CGCG 1 cut(s) 524
BshFI GGCC 2 cut(s) 303, 998
BshNI GGYRCC 1 cut(s) 202
BsiHKAI GWGCWC 1 cut(s) 436
BsiSI CCGG 1 cut(s) 773
BslI CCNNNNNNNGG 1 cut(s) 1124
BsmAI GTCTC 1 cut(s) 967
BsnI GGCC 2 cut(s) 303, 998
Bsp1286I GDGCHC 3 cut(s) 436, 825, 1078
Bsp143I GATC 4 cut(s) 73, 79, 325, 1148
Bsp19I CCATGG 1 cut(s) 664
BspACI CCGC 3 cut(s) 168, 766, 1057
BspANI GGCC 2 cut(s) 303, 998
BspCNI CTCAG 5 cut(s) 113, 450, 464, 465, 746
BspFNI CGCG 1 cut(s) 524
BspHI TCATGA 3 cut(s) 82, 118, 991
BspLI GGNNCC 3 cut(s) 204, 479, 568
BspMAI CTGCAG 2 cut(s) 810, 1300
BspPI GGATC 2 cut(s) 87, 1156
BspQI GCTCTTC 1 cut(s) 891
BspT107I GGYRCC 1 cut(s) 202
BsrFI RCCGGY 1 cut(s) 772
BsrI ACTGG 5 cut(s) 275, 379, 505, 863, 1230
BssAI RCCGGY 1 cut(s) 772
BssECI CCNNGG 1 cut(s) 664
BssMI GATC 4 cut(s) 73, 79, 325, 1148
BssT1I CCWWGG 1 cut(s) 664
Bst2UI CCWGG 1 cut(s) 608
Bst4CI ACNGT 4 cut(s) 112, 357, 756, 877
Bst6I CTCTTC 1 cut(s) 891
BstC8I GCNNGC 4 cut(s) 740, 774, 1034, 1275
BstDEI CTNAG 7 cut(s) 100, 451, 458, 473, 636, 733, 902
BstDSI CCRYGG 1 cut(s) 664
BstFNI CGCG 1 cut(s) 524
BstKTI GATC 4 cut(s) 76, 82, 328, 1151
BstMAI GTCTC 1 cut(s) 967
BstMBI GATC 4 cut(s) 73, 79, 325, 1148
BstMWI GCNNNNNNNGC 5 cut(s) 139, 165, 701, 710, 869
BstNI CCWGG 1 cut(s) 608
BstNSI RCATGY 1 cut(s) 1036
BstSCI CCNGG 1 cut(s) 606
BstSFI CTRYAG 2 cut(s) 806, 1296
BstUI CGCG 1 cut(s) 524
BstV1I GCAGC 3 cut(s) 142, 268, 1121
BstX2I RGATCY 1 cut(s) 325
BstYI RGATCY 1 cut(s) 325
Bsu36I CCTNAGG 1 cut(s) 733
BsuRI GGCC 2 cut(s) 303, 998
BtgI CCRYGG 1 cut(s) 664
BtsI GCAGTG 1 cut(s) 936
BtsIMutI CAGTG 3 cut(s) 470, 498, 936
Cac8I GCNNGC 4 cut(s) 740, 774, 1034, 1275
CciI TCATGA 3 cut(s) 82, 118, 991
Cfr10I RCCGGY 1 cut(s) 772
Cfr13I GGNCC 5 cut(s) 8, 566, 908, 925, 997
Csp6I GTAC 3 cut(s) 92, 614, 856
CviQI GTAC 3 cut(s) 92, 614, 856
DdeI CTNAG 7 cut(s) 100, 451, 458, 473, 636, 733, 902
DpnI GATC 4 cut(s) 75, 81, 327, 1150
DpnII GATC 4 cut(s) 73, 79, 325, 1148
EaeI YGGCCR 1 cut(s) 301
Eam1104I CTCTTC 1 cut(s) 891
EarI CTCTTC 1 cut(s) 891
Eco130I CCWWGG 1 cut(s) 664
Eco24I GRGCYC 2 cut(s) 825, 1078
Eco47I GGWCC 4 cut(s) 8, 566, 908, 925
Eco57I CTGAAG 1 cut(s) 915
Eco81I CCTNAGG 1 cut(s) 733
EcoO109I RGGNCCY 2 cut(s) 925, 997
EcoRII CCWGG 1 cut(s) 606
EcoT14I CCWWGG 1 cut(s) 664
EcoT38I GRGCYC 2 cut(s) 825, 1078
ErhI CCWWGG 1 cut(s) 664
FauNDI CATATG 1 cut(s) 972
FbaI TGATCA 1 cut(s) 73
Fnu4HI GCNGC 3 cut(s) 131, 282, 1110
FriOI GRGCYC 2 cut(s) 825, 1078
Fsp4HI GCNGC 3 cut(s) 131, 282, 1110
FspBI CTAG 3 cut(s) 422, 617, 1221
GluI GCNGC 3 cut(s) 131, 282, 1110
GsuI CTGGAG 2 cut(s) 522, 1247
HaeIII GGCC 2 cut(s) 303, 998
HapII CCGG 1 cut(s) 773
HincII GTYRAC 2 cut(s) 759, 1315
HindII GTYRAC 2 cut(s) 759, 1315
HinfI GANTC 5 cut(s) 122, 309, 464, 840, 1062
HpaII CCGG 1 cut(s) 773
HphI GGTGA 1 cut(s) 163
Hpy166II GTNNAC 4 cut(s) 759, 1138, 1155, 1315
Hpy188I TCNGA 5 cut(s) 195, 317, 454, 883, 1073
Hpy188III TCNNGA 6 cut(s) 77, 83, 119, 385, 893, 992
Hpy8I GTNNAC 4 cut(s) 759, 1138, 1155, 1315
HpyAV CCTTC 5 cut(s) 8, 706, 899, 1088, 1210
HpyCH4III ACNGT 4 cut(s) 112, 357, 756, 877
HpyCH4V TGCA 8 cut(s) 704, 726, 808, 818, 872, 1036, 1298, 1343
HpyF10VI GCNNNNNNNGC 5 cut(s) 139, 165, 701, 710, 869
HpyF3I CTNAG 7 cut(s) 100, 451, 458, 473, 636, 733, 902
KroI GCCGGC 1 cut(s) 772
KroNI GCCGGC 1 cut(s) 774
Ksp22I TGATCA 1 cut(s) 73
Kzo9I GATC 4 cut(s) 73, 79, 325, 1148
LguI GCTCTTC 1 cut(s) 891
LmnI GCTCC 2 cut(s) 483, 988
Lsp1109I GCAGC 3 cut(s) 142, 268, 1121
LweI GCATC 2 cut(s) 135, 186
MaeI CTAG 3 cut(s) 422, 617, 1221
MaeIII GTNAC 8 cut(s) 17, 151, 170, 505, 580, 778, 1102, 1282
MalI GATC 4 cut(s) 75, 81, 327, 1150
MboI GATC 4 cut(s) 73, 79, 325, 1148
MboII GAAGA 3 cut(s) 386, 410, 908
MfeI CAATTG 1 cut(s) 163
MflI RGATCY 1 cut(s) 325
MhlI GDGCHC 3 cut(s) 436, 825, 1078
MlsI TGGCCA 1 cut(s) 303
MluCI AATT 5 cut(s) 163, 705, 987, 1290, 1333
MluNI TGGCCA 1 cut(s) 303
MlyI GAGTC 1 cut(s) 849
MmeI TCCRAC 2 cut(s) 194, 560
Mox20I TGGCCA 1 cut(s) 303
MroNI GCCGGC 1 cut(s) 772
MroXI GAANNNNTTC 1 cut(s) 888
MscI TGGCCA 1 cut(s) 303
MseI TTAA 4 cut(s) 44, 447, 621, 921
MslI CAYNNNNRTG 4 cut(s) 87, 503, 785, 1100
Msp20I TGGCCA 1 cut(s) 303
MspI CCGG 1 cut(s) 773
MspR9I CCNGG 1 cut(s) 608
MunI CAATTG 1 cut(s) 163
MvaI CCWGG 1 cut(s) 608
MvnI CGCG 1 cut(s) 524
MwoI GCNNNNNNNGC 5 cut(s) 139, 165, 701, 710, 869
NaeI GCCGGC 1 cut(s) 774
NcoI CCATGG 1 cut(s) 664
NdeI CATATG 1 cut(s) 972
NdeII GATC 4 cut(s) 73, 79, 325, 1148
NgoMIV GCCGGC 1 cut(s) 772
NlaIV GGNNCC 3 cut(s) 204, 479, 568
NmuCI GTSAC 5 cut(s) 151, 505, 580, 778, 1102
NspI RCATGY 1 cut(s) 1036
OliI CACNNNNGTG 2 cut(s) 503, 1100
PaeI GCATGC 1 cut(s) 1036
PagI TCATGA 3 cut(s) 82, 118, 991
PciSI GCTCTTC 1 cut(s) 891
PdiI GCCGGC 1 cut(s) 774
PdmI GAANNNNTTC 1 cut(s) 888
PfeI GAWTC 4 cut(s) 122, 309, 464, 1062
PkrI GCNGC 3 cut(s) 132, 283, 1111
PleI GAGTC 1 cut(s) 848
PpsI GAGTC 1 cut(s) 848
PpuMI RGGWCCY 1 cut(s) 925
Psp5II RGGWCCY 1 cut(s) 925
Psp6I CCWGG 1 cut(s) 606
PspGI CCWGG 1 cut(s) 606
PspN4I GGNNCC 3 cut(s) 204, 479, 568
PspPI GGNCC 5 cut(s) 8, 566, 908, 925, 997
PspPPI RGGWCCY 1 cut(s) 925
PstI CTGCAG 2 cut(s) 810, 1300
PsuI RGATCY 1 cut(s) 325
RsaI GTAC 3 cut(s) 93, 615, 857
RsaNI GTAC 3 cut(s) 92, 614, 856
RseI CAYNNNNRTG 4 cut(s) 87, 503, 785, 1100
SapI GCTCTTC 1 cut(s) 891
SaqAI TTAA 4 cut(s) 44, 447, 621, 921
SatI GCNGC 3 cut(s) 131, 282, 1110
Sau3AI GATC 4 cut(s) 73, 79, 325, 1148
Sau96I GGNCC 5 cut(s) 8, 566, 908, 925, 997
SchI GAGTC 1 cut(s) 849
ScrFI CCNGG 1 cut(s) 608
SduI GDGCHC 3 cut(s) 436, 825, 1078
SfaNI GCATC 2 cut(s) 135, 186
SfcI CTRYAG 2 cut(s) 806, 1296
SinI GGWCC 4 cut(s) 8, 566, 908, 925
SmiMI CAYNNNNRTG 4 cut(s) 87, 503, 785, 1100
SmlI CTYRAG 1 cut(s) 437
SmoI CTYRAG 1 cut(s) 437
SpeI ACTAGT 1 cut(s) 616
SphI GCATGC 1 cut(s) 1036
Sse9I AATT 5 cut(s) 163, 705, 987, 1290, 1333
SsiI CCGC 3 cut(s) 168, 766, 1057
SspMI CTAG 3 cut(s) 422, 617, 1221
StyD4I CCNGG 1 cut(s) 606
StyI CCWWGG 1 cut(s) 664
TaaI ACNGT 4 cut(s) 112, 357, 756, 877
TaqI TCGA 2 cut(s) 61, 1040
TasI AATT 5 cut(s) 163, 705, 987, 1290, 1333
TatI WGTACW 1 cut(s) 91
TfiI GAWTC 4 cut(s) 122, 309, 464, 1062
Tru1I TTAA 4 cut(s) 44, 447, 621, 921
Tru9I TTAA 4 cut(s) 44, 447, 621, 921
TscAI CASTG 3 cut(s) 477, 505, 943
TseFI GTSAC 5 cut(s) 151, 505, 580, 778, 1102
TseI GCWGC 3 cut(s) 130, 281, 1109
Tsp45I GTSAC 5 cut(s) 151, 505, 580, 778, 1102
TspRI CASTG 3 cut(s) 477, 505, 943
VpaK11BI GGWCC 4 cut(s) 8, 566, 908, 925
XceI RCATGY 1 cut(s) 1036
XmnI GAANNNNTTC 1 cut(s) 888
XspI CTAG 3 cut(s) 422, 617, 1221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.