Rorug06G0512500

Proline transporter 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0011559
Physical Location & Seq
Forward (+)
62694508 .. 62697674
3167 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0512500.1

Sequence Viewer

Length: 1806 bp
ATGCCCTCACAGAAACCCCGCCATTTTCTCTCTCTGGCCCTCCTCATCTTCTTCAAGCCAAACCCTAACCCTAACTTTAACTTTCGTATTCTCTCTCTTCGATCCTTCTCCGACCAATCATGGCTGTCCGTACCCGGAAACCCCCTAATCAAGTGGCCCTCGCTCTCACCGCCTCCATCTCTCCCTTCCCCTTCCCCTAACCCTAATTTTGACCCCAAATTTTCACAAAATGACTTGTCCGCAATCGCTAACCTGCTCGCCGACCCACCCGGTCCATCGCTTCGGACCGCATTGGACCGGGCCGGAATTGACCCGAGTCCGAGTCTGCTACAGGCTGTGTTCGACCACTTTGATTCGTCTCCCAAATTGCTGCACACGCTGTTTGTTTGGGCTGAGAAGCAGCCCGGGTTTCGATGCTCTGCGACGCTCTTCACCTCCATGGTCAATGTGCTTGCCAAGGCTAGGGACTTTGAGTCTGCGTGGTCTATGATTCTTGATCGAATCGGCGGAGATAAGGAGCCTGGGTTGGTTTCTGTAGATGCATTTGTCATCATGATCAGACGGTATGCTCGTGCAGGTCAACCCCAATCAGCAATTCGAGCTTTTGAATTTGCAAGTACCTTAGAGTCATTTCTCAATTCTGAATCAGAAATGAGTTTGTTTGGAATCTTACTGGATTCCCTTTGTAAGGAGGGTCTTGTGAGGGTGGCCTCGGAATATTTTGATGGGAAAAGAAAGTTGCATCGAGATTGGATTCCATCGGTTCGGGTTTATAATATACTGTTGAATGGATGGTTTCGAGCGAGGAAGCTGAAGAAGGCAGAGCGGCTTTGGGTGGAGATGAAGAGAGACAGCGTGAAACCTAGTGTCGTGACATATGGTACACTTGTAGAAGGATACTGTCGGATGCGTCGTCCTGAAATCTCAATGGAGTTGGTGGGTGAGATGAGGAGGGAAGGAATTGAACCAAATGCGATTGTGTTTAATCCAATAATTGATGCTTTGGGGGAAGCCGGGAGGTTCAAGGAAGCGTGGGGGATGATGGAGCGGTTTTCAGTTCTGGAATCAGGCCCCACTATTTCGACGTACAATTCTCTGGTAAAGGGCTATTGCAAGGCCGGAAATCTTGTAGAAGCTAGTAGGATTATTAAGATGATGATAAGCAGGGGTATTGTACCAACTCCAACAACCTATAACTATTTCTTTAGGTTCTTTTCAAAGTCTGGGAAAATTGAGGAAGGAATGAACCTTTATACCAAAATGATTGAATCTGGGTATACCCCAGATCGTCTTACTTTCCATCTTCTGTTGAAGATGTTATGTGAAGAGGGAAGATTGGATTTGGCTGTTCAAGTTAGCAAGGAAATGAGAACTAGGGGATGTGATATGGATTTAGCCACGAGTACCATGTTGATTCATTTGCTCTGCAAGATGAATAAATTTAAAGAAGCTTTGTCAGAGTTTGAGGACATGATATGCAGGGGTTTAGTTCCGCAGTATCTAACTTTTCAGAACATGTATGATGAACTAAGGAAACAAGGAATGACTGAAATGGCACGAAAGCTGTGCGCCCTGATGTCTTCTGTTCCTCATTCAACAAAGTTGCCGAATACATATGTCAAAGATAGAGATGAATCTCATGCAAGGAGAAAATCTATAATTCAGAAGGCTGAAGCAATGTCTAATGTGTTGAAAACTTGTTCAGATCCAAGAGAACTTGTTAAGCACAGAAGTTCGCCGGAGACTGTTGAATCAAGAGCAAACCAGTTAATAGAGAATATTAAGACAAAAGCTAACATTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

601

Amino Acids

68.2

Weight (kDa)

9.32

Isoelectric Point (pI)

47.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 253 - 302 2.1e-14 PPR repeat family
PPR_3 PF13812 253 - 297 1.6e-06 Pentatricopeptide repeat domain
PPR_3 PF13812 277 - 337 8.9e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 286 - 317 3.1e-07 PPR repeat
PPR_2 PF13041 288 - 335 1.6e-09 PPR repeat family
PPR PF01535 291 - 321 9.9e-06 PPR repeat
PPR_2 PF13041 358 - 405 1.8e-10 PPR repeat family
PPR_1 PF12854 358 - 386 4.5e-08 PPR repeat
PPR_3 PF13812 360 - 404 5.4e-06 Pentatricopeptide repeat domain
PPR PF01535 361 - 391 2.9e-08 PPR repeat
PPR_2 PF13041 397 - 441 7.2e-08 PPR repeat family
PPR_2 PF13041 433 - 477 5.7e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 774
Acc36I ACCTGC 2 cut(s) 261, 566
AccBSI CCGCTC 2 cut(s) 826, 1048
AccI GTMKAC 1 cut(s) 1277
AciI CCGC 8 cut(s) 19, 170, 240, 288, 507, 826, 1048, 1493
AclWI GGATC 2 cut(s) 96, 1700
AcsI RAATTY 3 cut(s) 218, 608, 1439
AcuI CTGAAG 2 cut(s) 833, 1692
AfaI GTAC 6 cut(s) 132, 619, 883, 1088, 1176, 1405
AfiI CCNNNNNNNGG 2 cut(s) 462, 688
AflIII ACRYGT 1 cut(s) 1515
AhdI GACNNNNNGTC 1 cut(s) 472
AjnI CCWGG 1 cut(s) 520
AjuI GAANNNNNNNTTGG 2 cut(s) 579, 611
AluBI AGCT 6 cut(s) 602, 811, 1136, 1451, 1564, 1793
AluI AGCT 6 cut(s) 602, 811, 1136, 1451, 1564, 1793
Alw26I GTCTC 3 cut(s) 363, 843, 1736
AlwI GGATC 2 cut(s) 96, 1700
Ama87I CYCGRG 2 cut(s) 313, 404
AoxI GGCC 6 cut(s) 36, 155, 300, 708, 1069, 1116
ApeKI GCWGC 2 cut(s) 370, 400
ApoI RAATTY 3 cut(s) 218, 608, 1439
AspLEI GCGC 1 cut(s) 1571
AspS9I GGNCC 7 cut(s) 37, 156, 272, 285, 295, 300, 1070
AsuC2I CCSGG 6 cut(s) 135, 270, 299, 405, 406, 1015
AsuHPI GGTGA 3 cut(s) 159, 424, 953
AvaI CYCGRG 2 cut(s) 313, 404
AvaII GGWCC 3 cut(s) 272, 285, 295
BaeI ACNNNNGTAYC 2 cut(s) 873, 906
BauI CACGAG 2 cut(s) 570, 1399
BbsI GAAGAC 1 cut(s) 1572
BbvI GCAGC 2 cut(s) 357, 412
BccI CCATC 7 cut(s) 184, 283, 719, 766, 786, 1036, 1308
BcgI CGANNNNNNTGC 2 cut(s) 1548, 1582
BciT130I CCWGG 1 cut(s) 522
BciVI GTATCC 1 cut(s) 890
BclI TGATCA 1 cut(s) 555
BcnI CCSGG 6 cut(s) 135, 270, 299, 405, 406, 1015
BcoDI GTCTC 3 cut(s) 363, 843, 1736
BfaI CTAG 4 cut(s) 462, 864, 1137, 1374
BfmI CTRYAG 2 cut(s) 329, 534
BfuAI ACCTGC 2 cut(s) 261, 566
BfuI GTATCC 1 cut(s) 890
BisI GCNGC 3 cut(s) 371, 401, 827
BlsI GCNGC 3 cut(s) 372, 402, 828
Bme1390I CCNGG 7 cut(s) 135, 270, 299, 405, 406, 522, 1015
Bme18I GGWCC 3 cut(s) 272, 285, 295
BmeRI GACNNNNNGTC 1 cut(s) 472
BmeT110I CYCGRG 2 cut(s) 313, 404
BmgT120I GGNCC 7 cut(s) 37, 156, 272, 285, 295, 300, 1070
BmiI GGNNCC 2 cut(s) 519, 1072
BmrFI CCNGG 7 cut(s) 135, 270, 299, 405, 406, 522, 1015
BmsI GCATC 5 cut(s) 404, 529, 751, 897, 988
BoxI GACNNNNGTC 1 cut(s) 315
BpiI GAAGAC 1 cut(s) 1572
BpuMI CCSGG 6 cut(s) 135, 270, 299, 405, 406, 1015
BsaJI CCNNGG 5 cut(s) 404, 438, 456, 521, 711
BsaXI ACNNNNNCTCC 2 cut(s) 509, 539
Bsc4I CCNNNNNNNGG 2 cut(s) 462, 688
Bse1I ACTGG 2 cut(s) 678, 1765
Bse3DI GCAATG 1 cut(s) 1683
BseBI CCWGG 1 cut(s) 522
BseDI CCNNGG 5 cut(s) 404, 438, 456, 521, 711
BseGI GGATG 4 cut(s) 797, 912, 1044, 1385
BseLI CCNNNNNNNGG 2 cut(s) 462, 688
BseMI GCAATG 1 cut(s) 1683
BseMII CTCAG 1 cut(s) 384
BseNI ACTGG 2 cut(s) 678, 1765
BseRI GAGGAG 2 cut(s) 32, 964
BseXI GCAGC 2 cut(s) 357, 412
BsgI GTGCAG 2 cut(s) 356, 594
BshFI GGCC 6 cut(s) 38, 157, 302, 710, 1071, 1118
BsiHKCI CYCGRG 2 cut(s) 313, 404
BsiSI CCGG 8 cut(s) 135, 270, 298, 303, 405, 1014, 1119, 1739
BslFI GGGAC 1 cut(s) 479
BslI CCNNNNNNNGG 2 cut(s) 462, 688
BsmAI GTCTC 3 cut(s) 363, 843, 1736
BsmBI CGTCTC 1 cut(s) 363
BsmFI GGGAC 1 cut(s) 479
BsnI GGCC 6 cut(s) 38, 157, 302, 710, 1071, 1118
BsoBI CYCGRG 2 cut(s) 313, 404
Bsp143I GATC 5 cut(s) 101, 496, 555, 1285, 1705
Bsp19I CCATGG 1 cut(s) 438
BspACI CCGC 8 cut(s) 19, 170, 240, 288, 507, 826, 1048, 1493
BspANI GGCC 6 cut(s) 38, 157, 302, 710, 1071, 1118
BspCNI CTCAG 1 cut(s) 385
BspHI TCATGA 1 cut(s) 552
BspLI GGNNCC 2 cut(s) 519, 1072
BspMI ACCTGC 2 cut(s) 261, 566
BspPI GGATC 2 cut(s) 96, 1700
BspQI GCTCTTC 1 cut(s) 434
BsrBI CCGCTC 2 cut(s) 826, 1048
BsrDI GCAATG 1 cut(s) 1683
BsrI ACTGG 2 cut(s) 678, 1765
BssECI CCNNGG 5 cut(s) 404, 438, 456, 521, 711
BssMI GATC 5 cut(s) 101, 496, 555, 1285, 1705
BssNAI GTATAC 1 cut(s) 1278
BssSI CACGAG 2 cut(s) 570, 1399
BssT1I CCWWGG 2 cut(s) 438, 456
Bst1107I GTATAC 1 cut(s) 1278
Bst2BI CACGAG 2 cut(s) 570, 1399
Bst2UI CCWGG 1 cut(s) 522
Bst4CI ACNGT 4 cut(s) 564, 783, 902, 1747
Bst6I CTCTTC 4 cut(s) 102, 434, 839, 1320
BstC8I GCNNGC 2 cut(s) 258, 453
BstDEI CTNAG 3 cut(s) 393, 622, 1529
BstDSI CCRYGG 1 cut(s) 438
BstENI CCTNNNNNAGG 1 cut(s) 686
BstF5I GGATG 4 cut(s) 797, 912, 1044, 1385
BstHHI GCGC 1 cut(s) 1571
BstKTI GATC 5 cut(s) 104, 499, 558, 1288, 1708
BstMAI GTCTC 3 cut(s) 363, 843, 1736
BstMBI GATC 5 cut(s) 101, 496, 555, 1285, 1705
BstMWI GCNNNNNNNGC 3 cut(s) 169, 376, 599
BstNI CCWGG 1 cut(s) 522
BstNSI RCATGY 1 cut(s) 1519
BstPAI GACNNNNGTC 1 cut(s) 315
BstSCI CCNGG 7 cut(s) 133, 268, 297, 403, 404, 520, 1013
BstSFI CTRYAG 2 cut(s) 329, 534
BstV1I GCAGC 2 cut(s) 357, 412
BstV2I GAAGAC 1 cut(s) 1572
BstX2I RGATCY 1 cut(s) 1705
BstYI RGATCY 1 cut(s) 1705
BstZ17I GTATAC 1 cut(s) 1278
BsuI GTATCC 1 cut(s) 890
BsuRI GGCC 6 cut(s) 38, 157, 302, 710, 1071, 1118
BtgI CCRYGG 1 cut(s) 438
BtgZI GCGATG 1 cut(s) 261
BtsCI GGATG 4 cut(s) 797, 912, 1044, 1385
BveI ACCTGC 2 cut(s) 261, 566
Cac8I GCNNGC 2 cut(s) 258, 453
CciI TCATGA 1 cut(s) 552
CfoI GCGC 1 cut(s) 1571
Cfr13I GGNCC 7 cut(s) 37, 156, 272, 285, 295, 300, 1070
Cfr9I CCCGGG 1 cut(s) 404
CpoI CGGWCCG 1 cut(s) 285
CseI GACGC 2 cut(s) 433, 899
Csp6I GTAC 6 cut(s) 131, 618, 882, 1087, 1175, 1404
CspI CGGWCCG 1 cut(s) 285
CviAII CATG 7 cut(s) 120, 439, 553, 1408, 1471, 1516, 1640
CviQI GTAC 6 cut(s) 131, 618, 882, 1087, 1175, 1404
DdeI CTNAG 3 cut(s) 393, 622, 1529
DpnI GATC 5 cut(s) 103, 498, 557, 1287, 1707
DpnII GATC 5 cut(s) 101, 496, 555, 1285, 1705
DraI TTTAAA 1 cut(s) 1444
DriI GACNNNNNGTC 1 cut(s) 472
Eam1104I CTCTTC 4 cut(s) 102, 434, 839, 1320
Eam1105I GACNNNNNGTC 1 cut(s) 472
EarI CTCTTC 4 cut(s) 102, 434, 839, 1320
EciI GGCGGA 1 cut(s) 522
Eco130I CCWWGG 2 cut(s) 438, 456
Eco47I GGWCC 3 cut(s) 272, 285, 295
Eco57I CTGAAG 2 cut(s) 833, 1692
Eco88I CYCGRG 2 cut(s) 313, 404
EcoNI CCTNNNNNAGG 1 cut(s) 686
EcoO109I RGGNCCY 1 cut(s) 1070
EcoRII CCWGG 1 cut(s) 520
EcoT14I CCWWGG 2 cut(s) 438, 456
EcoT22I ATGCAT 1 cut(s) 544
ErhI CCWWGG 2 cut(s) 438, 456
Esp3I CGTCTC 1 cut(s) 363
FaeI CATG 7 cut(s) 123, 442, 556, 1411, 1474, 1519, 1643
FaqI GGGAC 1 cut(s) 479
FatI CATG 7 cut(s) 119, 438, 552, 1407, 1470, 1515, 1639
FauI CCCGC 1 cut(s) 26
FauNDI CATATG 2 cut(s) 877, 1615
FbaI TGATCA 1 cut(s) 555
FblI GTMKAC 1 cut(s) 1277
Fnu4HI GCNGC 3 cut(s) 371, 401, 827
FokI GGATG 4 cut(s) 804, 919, 1051, 1392
Fsp4HI GCNGC 3 cut(s) 371, 401, 827
FspBI CTAG 4 cut(s) 462, 864, 1137, 1374
GlaI GCGC 1 cut(s) 1570
GluI GCNGC 3 cut(s) 371, 401, 827
HaeIII GGCC 6 cut(s) 38, 157, 302, 710, 1071, 1118
HapII CCGG 8 cut(s) 135, 270, 298, 303, 405, 1014, 1119, 1739
HgaI GACGC 2 cut(s) 433, 899
HhaI GCGC 1 cut(s) 1571
Hin1II CATG 7 cut(s) 123, 442, 556, 1411, 1474, 1519, 1643
Hin6I GCGC 1 cut(s) 1569
HinP1I GCGC 1 cut(s) 1569
HincII GTYRAC 1 cut(s) 581
HindII GTYRAC 1 cut(s) 581
HindIII AAGCTT 1 cut(s) 1449
HpaII CCGG 8 cut(s) 135, 270, 298, 303, 405, 1014, 1119, 1739
HphI GGTGA 3 cut(s) 159, 424, 953
Hpy166II GTNNAC 3 cut(s) 581, 884, 1278
Hpy188III TCNNGA 7 cut(s) 494, 553, 746, 871, 917, 1061, 1755
Hpy8I GTNNAC 3 cut(s) 581, 884, 1278
Hpy99I CGWCG 3 cut(s) 427, 915, 1087
HpyAV CCTTC 8 cut(s) 115, 195, 201, 811, 887, 950, 1232, 1660
HpyCH4III ACNGT 4 cut(s) 564, 783, 902, 1747
HpyCH4IV ACGT 1 cut(s) 1085
HpyCH4V TGCA 9 cut(s) 373, 542, 575, 614, 742, 1113, 1428, 1479, 1643
HpyF10VI GCNNNNNNNGC 3 cut(s) 169, 376, 599
HpyF3I CTNAG 3 cut(s) 393, 622, 1529
HpySE526I ACGT 1 cut(s) 1085
Hsp92II CATG 7 cut(s) 123, 442, 556, 1411, 1474, 1519, 1643
HspAI GCGC 1 cut(s) 1569
Ksp22I TGATCA 1 cut(s) 555
Kzo9I GATC 5 cut(s) 101, 496, 555, 1285, 1705
LguI GCTCTTC 1 cut(s) 434
LmnI GCTCC 2 cut(s) 517, 1045
Lsp1109I GCAGC 2 cut(s) 357, 412
LweI GCATC 5 cut(s) 404, 529, 751, 897, 988
MaeI CTAG 4 cut(s) 462, 864, 1137, 1374
MaeII ACGT 1 cut(s) 1085
MaeIII GTNAC 1 cut(s) 871
MalI GATC 5 cut(s) 103, 498, 557, 1287, 1707
MbiI CCGCTC 2 cut(s) 826, 1048
MboI GATC 5 cut(s) 101, 496, 555, 1285, 1705
MflI RGATCY 1 cut(s) 1705
MlyI GAGTC 4 cut(s) 325, 331, 482, 635
MmeI TCCRAC 3 cut(s) 135, 884, 1208
Mph1103I ATGCAT 1 cut(s) 544
MseI TTAA 8 cut(s) 78, 984, 1149, 1443, 1722, 1769, 1782, 1800
MslI CAYNNNNRTG 1 cut(s) 437
MspI CCGG 8 cut(s) 135, 270, 298, 303, 405, 1014, 1119, 1739
MspR9I CCNGG 7 cut(s) 135, 270, 299, 405, 406, 522, 1015
MvaI CCWGG 1 cut(s) 522
MwoI GCNNNNNNNGC 3 cut(s) 169, 376, 599
NciI CCSGG 6 cut(s) 135, 270, 299, 405, 406, 1015
NcoI CCATGG 1 cut(s) 438
NdeI CATATG 2 cut(s) 877, 1615
NdeII GATC 5 cut(s) 101, 496, 555, 1285, 1705
NlaIII CATG 7 cut(s) 123, 442, 556, 1411, 1474, 1519, 1643
NlaIV GGNNCC 2 cut(s) 519, 1072
NmuCI GTSAC 1 cut(s) 871
NsiI ATGCAT 1 cut(s) 544
NspI RCATGY 1 cut(s) 1519
PagI TCATGA 1 cut(s) 552
PciI ACATGT 1 cut(s) 1515
PciSI GCTCTTC 1 cut(s) 434
PcsI WCGNNNNNNNCGW 2 cut(s) 568, 910
PkrI GCNGC 3 cut(s) 372, 402, 828
PleI GAGTC 4 cut(s) 324, 330, 481, 634
PpsI GAGTC 4 cut(s) 324, 330, 481, 634
PscI ACATGT 1 cut(s) 1515
PshAI GACNNNNGTC 1 cut(s) 315
PsiI TTATAA 1 cut(s) 774
Psp6I CCWGG 1 cut(s) 520
PspGI CCWGG 1 cut(s) 520
PspN4I GGNNCC 2 cut(s) 519, 1072
PspPI GGNCC 7 cut(s) 37, 156, 272, 285, 295, 300, 1070
PsuI RGATCY 1 cut(s) 1705
RsaI GTAC 6 cut(s) 132, 619, 883, 1088, 1176, 1405
RsaNI GTAC 6 cut(s) 131, 618, 882, 1087, 1175, 1404
RseI CAYNNNNRTG 1 cut(s) 437
Rsr2I CGGWCCG 1 cut(s) 285
RsrII CGGWCCG 1 cut(s) 285
SapI GCTCTTC 1 cut(s) 434
SaqAI TTAA 8 cut(s) 78, 984, 1149, 1443, 1722, 1769, 1782, 1800
SatI GCNGC 3 cut(s) 371, 401, 827
Sau3AI GATC 5 cut(s) 101, 496, 555, 1285, 1705
Sau96I GGNCC 7 cut(s) 37, 156, 272, 285, 295, 300, 1070
SchI GAGTC 4 cut(s) 325, 331, 482, 635
ScrFI CCNGG 7 cut(s) 135, 270, 299, 405, 406, 522, 1015
SfaNI GCATC 5 cut(s) 404, 529, 751, 897, 988
SfcI CTRYAG 2 cut(s) 329, 534
SinI GGWCC 3 cut(s) 272, 285, 295
SmaI CCCGGG 1 cut(s) 406
SmiMI CAYNNNNRTG 1 cut(s) 437
SsiI CCGC 8 cut(s) 19, 170, 240, 288, 507, 826, 1048, 1493
SspI AATATT 2 cut(s) 719, 1780
SspMI CTAG 4 cut(s) 462, 864, 1137, 1374
StyD4I CCNGG 7 cut(s) 133, 268, 297, 403, 404, 520, 1013
StyI CCWWGG 2 cut(s) 438, 456
TaaI ACNGT 4 cut(s) 564, 783, 902, 1747
TaiI ACGT 1 cut(s) 1088
TaqI TCGA 8 cut(s) 100, 342, 412, 499, 598, 745, 799, 1082
TauI GCSGC 1 cut(s) 829
Tru1I TTAA 8 cut(s) 78, 984, 1149, 1443, 1722, 1769, 1782, 1800
Tru9I TTAA 8 cut(s) 78, 984, 1149, 1443, 1722, 1769, 1782, 1800
TseFI GTSAC 1 cut(s) 871
TseI GCWGC 2 cut(s) 370, 400
Tsp45I GTSAC 1 cut(s) 871
TspDTI ATGAA 6 cut(s) 857, 1259, 1406, 1448, 1539, 1647
TspGWI ACGGA 1 cut(s) 118
TspMI CCCGGG 1 cut(s) 404
VpaK11BI GGWCC 3 cut(s) 272, 285, 295
XagI CCTNNNNNAGG 1 cut(s) 686
XapI RAATTY 3 cut(s) 218, 608, 1439
XceI RCATGY 1 cut(s) 1519
XmaI CCCGGG 1 cut(s) 404
XmiI GTMKAC 1 cut(s) 1277
XspI CTAG 4 cut(s) 462, 864, 1137, 1374
Zsp2I ATGCAT 1 cut(s) 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.