Rh7AG122900

Proline transporter 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0011559
Physical Location & Seq
Forward (+)
9643059 .. 9645168
2110 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG122900.1

Sequence Viewer

Length: 1356 bp
ATGGAAGGACTAGAAGGCAACACAAACAACAATGAAGCTGGTTTGAGCATTGAACAAGCTCGAACAAAGTGTGATCAAGATAATCATGAATATACACTCCCCTCAGCTCACGCTATTGGTCATGATTCATGGCAGCAGGTTGGGTTGATGATGGTGACTGGCTTCAATTGCGGTTACATATTGAGCTTTTCAAATCTGATGCTTGTGCCTTTGGGTTGGACATGGGGTATCATCTGCCTCGTTGTTGTTGGCTTTTATACCGCCTATGCTAACTGGCTTTTGGCTGCTTTTCACTTCATCAATGGCCAAAGATTCATCAGATACAGAGATATTATGGGCTATTTATATGGCAGAGAAATGTATTACATAACATGGGTTTTACAGTTCTCGACCCTACTTCTTGGAAATATGGGTTTCATTCTTCTAGGGGGTAGAGCACTCAAGGAGATTAACTCAGGACTCAGTGATTCTCCACTGCGGCTCCAATACTTTATTGTCATCACCGGAGTGACCTACTTCATATTCGCCTTTTTCATTCCAACAATGTCTAAAATGAGATTTTGGCTTGGACCCTCTACCATTGTCACTTTTGCTTACATTGTCATACTCTTGGTGGTACTAGTTAAAGATGGGAAAACAAAGACGACAGAGCGAAATTATGAAATCCATGGAAACAAAGCTGAGAGAGTGTTCAATGCCTTCAATGCAGTTTCAGCTATTATTGTCTGCAACACCGCAGGCTTGCTACTAGAAATACAGTCAACCCTGCAGAAGCCAGCAGTAACAAATATGAGGAAAGCTTTATATCTGCAGTATAGTGCAGGGCTCTTATTTTACTATGGAGTCAGCATAGTAGGATACTGGGCATATGGCGCAACCGTATCTGAATACCTTCCTGAAGAGCTAAGTGGTCCTAAATCGGTTAAGGTCCTCATAAACGCTGCCGTGTTTCTACAATCCATAGTCTCACAACATATGTTTGTTGCACCAATTCATGAGGCCCTTGATACCAAGTTCCTTAAACTTGATGAGAGCATGCACTGGAAATATAACTTCAAGCGGAGGTTCTACCTCCGAGCCCTTCTCTTCATGGGAAATACATTTGTGACAGCAGCTATTCCTTTCATGGGGGACTTTGTGAACTTATTTGGATCGTTTACACTCGTTCCTCTAACTTTTGTTTTTCCAAGCATGATTTTCATTAAGGTGAAGGGAAAGACAGCTAGACTGGAGAAAAATTTGTGGCATTGGTTCAACATTGTTATTTTTTCTCTGCTTGCGATTATAACTACGATTTCTGCAGTTCGTTCGATTGTCAACAATGTTAAGGAATACAATTTCTTTGCAGATACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

451

Amino Acids

51.25

Weight (kDa)

9.1

Isoelectric Point (pI)

29.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 42 - 434 5.1e-59 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1286
Acc36I ACCTGC 1 cut(s) 127
AciI CCGC 5 cut(s) 171, 261, 478, 735, 1060
AclWI GGATC 1 cut(s) 1159
AcoI YGGCCR 1 cut(s) 304
AcsI RAATTY 1 cut(s) 1237
AcuI CTGAAG 1 cut(s) 918
AfaI GTAC 1 cut(s) 618
AfiI CCNNNNNNNGG 1 cut(s) 1127
AgsI TTSAA 7 cut(s) 53, 166, 192, 694, 703, 1057, 1255
AhlI ACTAGT 1 cut(s) 619
AleI CACNNNNGTG 1 cut(s) 506
Alw21I GWGCWC 1 cut(s) 439
Alw26I GTCTC 1 cut(s) 970
AlwI GGATC 1 cut(s) 1159
AoxI GGCC 2 cut(s) 304, 999
ApeKI GCWGC 4 cut(s) 133, 284, 941, 1112
ApoI RAATTY 1 cut(s) 1237
Asp700I GAANNNNTTC 1 cut(s) 891
AspLEI GCGC 1 cut(s) 875
AspS9I GGNCC 4 cut(s) 569, 911, 928, 1000
AsuHPI GGTGA 3 cut(s) 166, 493, 1219
AvaII GGWCC 3 cut(s) 569, 911, 928
BalI TGGCCA 1 cut(s) 306
BanII GRGCYC 2 cut(s) 828, 1081
Bbv12I GWGCWC 1 cut(s) 439
BbvCI CCTCAGC 1 cut(s) 103
BbvI GCAGC 4 cut(s) 145, 271, 928, 1124
BccI CCATC 2 cut(s) 145, 623
BceAI ACGGC 1 cut(s) 929
BcgI CGANNNNNNTGC 2 cut(s) 1290, 1324
BciVI GTATCC 1 cut(s) 851
BclI TGATCA 1 cut(s) 73
BcoDI GTCTC 1 cut(s) 970
BcuI ACTAGT 1 cut(s) 619
BfaI CTAG 5 cut(s) 11, 425, 620, 749, 1224
BfmI CTRYAG 3 cut(s) 767, 809, 1299
BfuAI ACCTGC 1 cut(s) 127
BfuI GTATCC 1 cut(s) 851
BisI GCNGC 5 cut(s) 134, 285, 479, 942, 1113
BlsI GCNGC 5 cut(s) 135, 286, 480, 943, 1114
Bme18I GGWCC 3 cut(s) 569, 911, 928
BmgT120I GGNCC 4 cut(s) 569, 911, 928, 1000
BmiI GGNNCC 2 cut(s) 482, 571
BmrI ACTGGG 1 cut(s) 871
BmsI GCATC 1 cut(s) 189
BmuI ACTGGG 1 cut(s) 871
BplI GAGNNNNNCTC 4 cut(s) 437, 469, 1068, 1100
BpmI CTGGAG 1 cut(s) 1250
Bpu10I CCTNAGC 1 cut(s) 103
BpuEI CTTGAG 1 cut(s) 425
BsaJI CCNNGG 1 cut(s) 667
BsaWI WCCGGW 1 cut(s) 503
BsaXI ACNNNNNCTCC 2 cut(s) 465, 495
Bsc4I CCNNNNNNNGG 1 cut(s) 1127
Bse1I ACTGG 5 cut(s) 163, 278, 866, 1046, 1233
BseDI CCNNGG 1 cut(s) 667
BseLI CCNNNNNNNGG 1 cut(s) 1127
BseMII CTCAG 4 cut(s) 117, 468, 475, 672
BseNI ACTGG 5 cut(s) 163, 278, 866, 1046, 1233
BseXI GCAGC 4 cut(s) 145, 271, 928, 1124
BsgI GTGCAG 1 cut(s) 840
BshFI GGCC 2 cut(s) 306, 1001
BsiHKAI GWGCWC 1 cut(s) 439
BsiSI CCGG 1 cut(s) 504
BslFI GGGAC 1 cut(s) 1145
BslI CCNNNNNNNGG 1 cut(s) 1127
BsmAI GTCTC 1 cut(s) 970
BsmFI GGGAC 1 cut(s) 1145
BsnI GGCC 2 cut(s) 306, 1001
Bsp1286I GDGCHC 3 cut(s) 439, 828, 1081
Bsp143I GATC 2 cut(s) 73, 1151
Bsp19I CCATGG 1 cut(s) 667
BspACI CCGC 5 cut(s) 171, 261, 478, 735, 1060
BspANI GGCC 2 cut(s) 306, 1001
BspCNI CTCAG 4 cut(s) 116, 467, 474, 673
BspHI TCATGA 3 cut(s) 85, 121, 994
BspLI GGNNCC 2 cut(s) 482, 571
BspMAI CTGCAG 3 cut(s) 771, 813, 1303
BspMI ACCTGC 1 cut(s) 127
BspPI GGATC 1 cut(s) 1159
BspQI GCTCTTC 1 cut(s) 894
BsrI ACTGG 5 cut(s) 163, 278, 866, 1046, 1233
BssECI CCNNGG 1 cut(s) 667
BssMI GATC 2 cut(s) 73, 1151
BssT1I CCWWGG 1 cut(s) 667
Bst4CI ACNGT 3 cut(s) 384, 759, 880
Bst6I CTCTTC 2 cut(s) 894, 1091
BstC8I GCNNGC 5 cut(s) 739, 743, 777, 1037, 1278
BstDEI CTNAG 5 cut(s) 103, 454, 461, 681, 905
BstDSI CCRYGG 1 cut(s) 667
BstHHI GCGC 1 cut(s) 875
BstKTI GATC 2 cut(s) 76, 1154
BstMAI GTCTC 1 cut(s) 970
BstMBI GATC 2 cut(s) 73, 1151
BstMWI GCNNNNNNNGC 4 cut(s) 168, 704, 713, 872
BstNSI RCATGY 1 cut(s) 1039
BstSFI CTRYAG 3 cut(s) 767, 809, 1299
BstV1I GCAGC 4 cut(s) 145, 271, 928, 1124
BsuI GTATCC 1 cut(s) 851
BsuRI GGCC 2 cut(s) 306, 1001
BtgI CCRYGG 1 cut(s) 667
BtsI GCAGTG 1 cut(s) 473
BtsIMutI CAGTG 3 cut(s) 469, 473, 1039
BveI ACCTGC 1 cut(s) 127
Cac8I GCNNGC 5 cut(s) 739, 743, 777, 1037, 1278
CciI TCATGA 3 cut(s) 85, 121, 994
CfoI GCGC 1 cut(s) 875
Cfr13I GGNCC 4 cut(s) 569, 911, 928, 1000
Csp6I GTAC 1 cut(s) 617
CviQI GTAC 1 cut(s) 617
DdeI CTNAG 5 cut(s) 103, 454, 461, 681, 905
DpnI GATC 2 cut(s) 75, 1153
DpnII GATC 2 cut(s) 73, 1151
EaeI YGGCCR 1 cut(s) 304
Eam1104I CTCTTC 2 cut(s) 894, 1091
EarI CTCTTC 2 cut(s) 894, 1091
Eco130I CCWWGG 1 cut(s) 667
Eco24I GRGCYC 2 cut(s) 828, 1081
Eco47I GGWCC 3 cut(s) 569, 911, 928
Eco57I CTGAAG 1 cut(s) 918
EcoO109I RGGNCCY 2 cut(s) 928, 1000
EcoT14I CCWWGG 1 cut(s) 667
EcoT38I GRGCYC 2 cut(s) 828, 1081
ErhI CCWWGG 1 cut(s) 667
FaqI GGGAC 1 cut(s) 1145
FauNDI CATATG 2 cut(s) 868, 975
FbaI TGATCA 1 cut(s) 73
Fnu4HI GCNGC 5 cut(s) 134, 285, 479, 942, 1113
FriOI GRGCYC 2 cut(s) 828, 1081
Fsp4HI GCNGC 5 cut(s) 134, 285, 479, 942, 1113
FspBI CTAG 5 cut(s) 11, 425, 620, 749, 1224
GlaI GCGC 1 cut(s) 874
GluI GCNGC 5 cut(s) 134, 285, 479, 942, 1113
GsuI CTGGAG 1 cut(s) 1250
HaeIII GGCC 2 cut(s) 306, 1001
HapII CCGG 1 cut(s) 504
HhaI GCGC 1 cut(s) 875
Hin6I GCGC 1 cut(s) 873
HinP1I GCGC 1 cut(s) 873
HincII GTYRAC 2 cut(s) 762, 1318
HindII GTYRAC 2 cut(s) 762, 1318
HindIII AAGCTT 1 cut(s) 798
HinfI GANTC 5 cut(s) 125, 312, 459, 467, 843
HpaII CCGG 1 cut(s) 504
HphI GGTGA 3 cut(s) 166, 493, 1219
Hpy166II GTNNAC 4 cut(s) 762, 1141, 1158, 1318
Hpy188I TCNGA 4 cut(s) 198, 320, 886, 1076
Hpy188III TCNNGA 7 cut(s) 77, 86, 122, 388, 456, 896, 995
Hpy8I GTNNAC 4 cut(s) 762, 1141, 1158, 1318
HpyAV CCTTC 5 cut(s) 8, 709, 902, 1091, 1204
HpyCH4III ACNGT 3 cut(s) 384, 759, 880
HpyCH4V TGCA 9 cut(s) 707, 729, 769, 811, 821, 986, 1039, 1301, 1346
HpyF10VI GCNNNNNNNGC 4 cut(s) 168, 704, 713, 872
HpyF3I CTNAG 5 cut(s) 103, 454, 461, 681, 905
HspAI GCGC 1 cut(s) 873
Ksp22I TGATCA 1 cut(s) 73
Kzo9I GATC 2 cut(s) 73, 1151
LguI GCTCTTC 1 cut(s) 894
LmnI GCTCC 1 cut(s) 486
Lsp1109I GCAGC 4 cut(s) 145, 271, 928, 1124
LweI GCATC 1 cut(s) 189
MaeI CTAG 5 cut(s) 11, 425, 620, 749, 1224
MaeIII GTNAC 6 cut(s) 154, 173, 508, 583, 781, 1105
MalI GATC 2 cut(s) 75, 1153
MboI GATC 2 cut(s) 73, 1151
MboII GAAGA 3 cut(s) 413, 911, 1078
MfeI CAATTG 1 cut(s) 166
MhlI GDGCHC 3 cut(s) 439, 828, 1081
MlsI TGGCCA 1 cut(s) 306
MluCI AATT 5 cut(s) 166, 655, 990, 1237, 1336
MluNI TGGCCA 1 cut(s) 306
MlyI GAGTC 2 cut(s) 453, 852
MmeI TCCRAC 2 cut(s) 197, 563
MnlI CCTC 9 cut(s) 112, 248, 583, 786, 941, 991, 1056, 1082, 1179
Mox20I TGGCCA 1 cut(s) 306
MroXI GAANNNNTTC 1 cut(s) 891
MscI TGGCCA 1 cut(s) 306
MseI TTAA 6 cut(s) 450, 624, 924, 1020, 1203, 1326
MslI CAYNNNNRTG 2 cut(s) 506, 1205
Msp20I TGGCCA 1 cut(s) 306
MspI CCGG 1 cut(s) 504
MunI CAATTG 1 cut(s) 166
MwoI GCNNNNNNNGC 4 cut(s) 168, 704, 713, 872
NcoI CCATGG 1 cut(s) 667
NdeI CATATG 2 cut(s) 868, 975
NdeII GATC 2 cut(s) 73, 1151
NlaIV GGNNCC 2 cut(s) 482, 571
NmuCI GTSAC 4 cut(s) 154, 508, 583, 1105
NspI RCATGY 1 cut(s) 1039
OliI CACNNNNGTG 1 cut(s) 506
PaeI GCATGC 1 cut(s) 1039
PagI TCATGA 3 cut(s) 85, 121, 994
PciSI GCTCTTC 1 cut(s) 894
PdmI GAANNNNTTC 1 cut(s) 891
PfeI GAWTC 3 cut(s) 125, 312, 467
PkrI GCNGC 5 cut(s) 135, 286, 480, 943, 1114
PleI GAGTC 2 cut(s) 453, 851
PpsI GAGTC 2 cut(s) 453, 851
PpuMI RGGWCCY 1 cut(s) 928
PsiI TTATAA 1 cut(s) 1286
Psp5II RGGWCCY 1 cut(s) 928
PspN4I GGNNCC 2 cut(s) 482, 571
PspPI GGNCC 4 cut(s) 569, 911, 928, 1000
PspPPI RGGWCCY 1 cut(s) 928
PstI CTGCAG 3 cut(s) 771, 813, 1303
RsaI GTAC 1 cut(s) 618
RsaNI GTAC 1 cut(s) 617
RseI CAYNNNNRTG 2 cut(s) 506, 1205
SapI GCTCTTC 1 cut(s) 894
SaqAI TTAA 6 cut(s) 450, 624, 924, 1020, 1203, 1326
SatI GCNGC 5 cut(s) 134, 285, 479, 942, 1113
Sau3AI GATC 2 cut(s) 73, 1151
Sau96I GGNCC 4 cut(s) 569, 911, 928, 1000
SchI GAGTC 2 cut(s) 453, 852
SduI GDGCHC 3 cut(s) 439, 828, 1081
SfaNI GCATC 1 cut(s) 189
SfcI CTRYAG 3 cut(s) 767, 809, 1299
SinI GGWCC 3 cut(s) 569, 911, 928
SmiMI CAYNNNNRTG 2 cut(s) 506, 1205
SmlI CTYRAG 1 cut(s) 440
SmoI CTYRAG 1 cut(s) 440
SpeI ACTAGT 1 cut(s) 619
SphI GCATGC 1 cut(s) 1039
Sse9I AATT 5 cut(s) 166, 655, 990, 1237, 1336
SsiI CCGC 5 cut(s) 171, 261, 478, 735, 1060
SspMI CTAG 5 cut(s) 11, 425, 620, 749, 1224
StyI CCWWGG 1 cut(s) 667
TaaI ACNGT 3 cut(s) 384, 759, 880
TaqI TCGA 3 cut(s) 61, 389, 1310
TasI AATT 5 cut(s) 166, 655, 990, 1237, 1336
TauI GCSGC 1 cut(s) 481
TfiI GAWTC 3 cut(s) 125, 312, 467
Tru1I TTAA 6 cut(s) 450, 624, 924, 1020, 1203, 1326
Tru9I TTAA 6 cut(s) 450, 624, 924, 1020, 1203, 1326
TscAI CASTG 3 cut(s) 469, 480, 1046
TseFI GTSAC 4 cut(s) 154, 508, 583, 1105
TseI GCWGC 4 cut(s) 133, 284, 941, 1112
Tsp45I GTSAC 4 cut(s) 154, 508, 583, 1105
TspRI CASTG 3 cut(s) 469, 480, 1046
VpaK11BI GGWCC 3 cut(s) 569, 911, 928
XapI RAATTY 1 cut(s) 1237
XceI RCATGY 1 cut(s) 1039
XmnI GAANNNNTTC 1 cut(s) 891
XspI CTAG 5 cut(s) 11, 425, 620, 749, 1224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.